CN114667891A - Novel edible mushroom cultivation bottle and use method thereof - Google Patents
Novel edible mushroom cultivation bottle and use method thereof Download PDFInfo
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- CN114667891A CN114667891A CN202210259644.7A CN202210259644A CN114667891A CN 114667891 A CN114667891 A CN 114667891A CN 202210259644 A CN202210259644 A CN 202210259644A CN 114667891 A CN114667891 A CN 114667891A
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G18/00—Cultivation of mushrooms
- A01G18/60—Cultivation rooms; Equipment therefor
- A01G18/64—Cultivation containers; Lids therefor
- A01G18/68—Cultivation bottles
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G18/00—Cultivation of mushrooms
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- Environmental Sciences (AREA)
- Mushroom Cultivation (AREA)
Abstract
The invention provides a novel edible fungus cultivation bottle and a using method thereof, and relates to the technical field of fungus cultivation. The novel edible fungus cultivation bottle comprises a cultivation bottle body, wherein a partition plate is fixedly connected to the middle lower portion of the inner side wall of the cultivation bottle body, grid holes which are uniformly arranged in a grid shape are formed in the partition plate, and a fixed cover ring is fixedly connected to an output port end of the top of the cultivation bottle body. The perforated grid structure is designed at the position 1cm away from the bottom of the cultivation bottle, the cultivation material is separated from the bottom of the bottle, accumulated water cannot directly soak the cultivation material, the accumulated water is reserved at the bottom of the mixed cultivation material and cannot flow into the bottom of the bottle, the phenomenon that hypha growth is influenced due to the accumulated water at the bottom caused by extrusion, sterilization and inoculation is avoided, meanwhile, redundant water at the bottom of the bottle can be slowly evaporated and absorbed by the cultivation material in the growth process of flammulina velutipes, and therefore water absorption of the roots of the body of the flammulina velutipes in the later growth stage is guaranteed, compactness and whiteness of the roots are improved, and unit yield and quality of products are improved.
Description
Technical Field
The invention relates to the technical field of fungus cultivation, in particular to a novel edible fungus cultivation bottle and a using method thereof.
Background
In recent years, the yield of the flammulina velutipes is doubled, the industry competition is intensified day by day, and the structure of the cultivation bottle needs to be further optimized in order to improve the quality and the unit yield of the flammulina velutipes. The water content is one of the most important nutrients in the growth process of the flammulina velutipes, and before strains are inoculated into the culture medium, the physiological requirements of the flammulina velutipes can be met only when the water content of the culture medium reaches 60-70%. The existing cultivation bottles are generally designed to be hollow, no other structure is arranged in the bottles, and cultivation materials are directly contacted with the bottle walls and the bottle walls after being filled into the cultivation bottles.
The existing cultivation bottle is filled with cultivation materials, water in the cultivation bottle migrates due to extrusion of punching equipment, the water content of the cultivation materials at the upper part, the middle part and the lower part is inconsistent, particularly, the water content at the bottom is usually higher than that at the middle upper part, water may enter the cultivation bottle in a high-temperature sterilization process, so that the water content at the bottom of the cultivation bottle is too high, hypha growth is influenced, if the water content at the bottom of the cultivation bottle is too high, accumulated water appears at the bottom, pollution is easy to cause, and hypha growth is influenced.
Disclosure of Invention
Aiming at the defects of the prior art, the invention provides a novel edible fungus cultivation bottle and a using method thereof, and solves the problem that the water content of the upper part, the middle part and the lower part of the existing edible fungus cultivation bottle is inconsistent during cultivation.
In order to achieve the purpose, the invention is realized by the following technical scheme: novel edible mushroom cultivation bottle, including the blake bottle body, lower part fixed connection divider disc in the blake bottle body inside wall, the inside latticed net hole of arranging that is provided with of divider disc, the fixed cover ring of blake bottle body top delivery outlet fixed connection, fixed cover ring top delivery outlet is provided with sealed lid, the aperture of latticed hole is 1.5 millimeters, the divider disc is fixed in the inside diapire top 1 centimetre department of blake bottle body apart.
Preferably, the use method of the novel edible fungus cultivation bottle is characterized in that: the method comprises the following steps:
s1, stirring raw materials
Placing planting and cultivating raw materials of edible fungi such as needle mushrooms into a culture medium, correspondingly adding water into the culture medium, and stirring and mixing at a rotating speed of 20-30 revolutions per minute to form a mixed cultivation material;
s2, bottling
After being stirred uniformly, the mixed cultivation material is filled into a culture bottle body and is carried by a separation disc;
S3, high-temperature sterilization
Sterilizing the culture bottle body carrying the mixed culture material at high temperature, and heating the culture bottle body for 1-1.5 hours at 80-95 ℃;
s4 inoculation
Punching two groups of cultivation holes with the diameter of 1-1.5 cm on the top of the mixed cultivation material in the cultivation bottle body, and injecting the flammulina velutipes strain liquid into the two groups of cultivation holes for a minute to finish cultivation;
s5, cultivation
Placing the culture flask body subjected to cultivation in an environment with the temperature of 5-25 ℃ for standing cultivation;
s6 mycelium stimulation
And when the hyphae in the culture bottle body grow to 3-5 cm, removing the inoculation blocks on the surface of the culture bottle body and the hyphae aged on the surface layer by using a sterilized mycelium scratching hook, finishing and leveling, and allowing the hyphae to naturally grow.
Preferably, in the step of S1, in the step of stirring the raw materials, water is continuously added during the stirring process, so that the water content of the culture medium is 60% to 70%.
Preferably, in the step of S2 bottling, the mixed cultivation material is supported by the separating tray and is kept at a distance of 1 cm from the cultivation bottle body.
Preferably, in the step S4, a part of the flammulina velutipes strain liquid is left on the top surface of the mixed cultivation material, and a part of the flammulina velutipes strain liquid flows into the bottom of the mixed cultivation material along the cultivation holes.
Preferably, in the step S3, in the sterilization process, if water enters, the water flows down along the mesh holes, and is filtered to the bottom of the culture flask body through the mesh, so that the culture material is not directly soaked by the accumulated water.
Preferably, in the step S4, during inoculation, hyphae and bacteria liquid are blocked by the grids due to the small diameter of the grid holes, and remain at the bottom of the mixed cultivation material, so as not to flow into the bottom of the bottle.
Preferably, in the step S6, the cleaning end of the mycelium stimulation hook needs to be bent to form a 90-degree angle.
The invention provides a novel edible mushroom cultivation bottle and a using method thereof. The method has the following beneficial effects:
according to the cultivation method, the cultivation material is separated from the bottle bottom by optimizing the design of the cultivation bottle, if water enters the cultivation bottle, the water flows down along the grid holes and is filtered to the bottom of the cultivation bottle body through the grid, accumulated water cannot directly soak the cultivation material, and in the inoculation process, as the diameters of the grid holes are smaller, hyphae and bacterial liquid are blocked by the grid and are retained at the bottom of the mixed cultivation material and cannot flow into the bottle bottom, the phenomenon that the hyphae grow due to the accumulated water at the bottom caused by extrusion, sterilization and inoculation is avoided, meanwhile, excessive moisture at the bottle bottom can be slowly evaporated and absorbed by the cultivation material in the growth process of the flammulina velutipes, so that the moisture absorption at the root of the body in the later growth stage is ensured, the compactness and whiteness of the root are improved, and the yield per unit and quality of products are improved.
Drawings
FIG. 1 is a front view of a cultivation flask according to the present invention;
FIG. 2 is an isometric view of a flask according to the present invention;
FIG. 3 is a schematic view of the upper and lower equiangular axes of the flask according to the present invention;
FIG. 4 is a schematic top view of the divider disk of the present invention.
Wherein, 1, culturing a bottle body; 2. a divider disk; 3. mesh holes; 4. fixing the cover ring; 5. and (7) sealing the cover.
Detailed Description
The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.
Example (b):
as shown in fig. 1-4, the embodiment of the present invention provides a novel edible mushroom cultivation bottle, which includes a cultivation bottle body 1, a separation disc 2 is fixedly connected to a middle lower portion of an inner side wall of the cultivation bottle body 1, grid holes 3 are uniformly arranged in the separation disc 2, a fixed cover ring 4 is fixedly connected to an output port at the top of the cultivation bottle body 1, a sealing cover 5 is arranged at an output port at the top of the fixed cover ring 4, before the cultivation bottle body 1 is used for cultivation, the sealing cover 5 can be sealed on the fixed cover ring 4, so that the inside of the cultivation bottle body 1 can be kept sealed, the aperture of the grid hole 3 is 1.5 mm, and the separation disc 2 is fixed at a position 1 cm above a bottom wall of the inside of the cultivation bottle body 1.
The novel edible fungus cultivation bottle using method comprises the following using steps:
s1, stirring the raw materials
Placing planting and cultivating raw materials of edible fungi such as flammulina velutipes into a culture medium, correspondingly adding water into the culture medium, stirring and mixing at a rotating speed of 20-30 revolutions per minute, and continuously adding water during stirring to enable the culture medium to reach a water content of 60-70% to form a mixed cultivating material;
s2, bottling
After being uniformly stirred, the mixed cultivation material is filled into the culture bottle body 1 and is carried by the separation disc 2, and the mixed cultivation material is kept at an interval of 1 cm with the culture bottle body 1 under the carrying of the separation disc 2;
s3, high-temperature sterilization
Sterilizing the culture bottle body 1 bearing the mixed culture material at high temperature, heating the culture bottle body 1 at 80-95 ℃ for 1-1.5 hours, wherein in the sterilization process, if water enters, the water flows down along the grid holes 3 and is filtered to the bottom of the culture bottle body 1 through the grid, and the culture material cannot be directly soaked in accumulated water;
s4, inoculation
Punching two groups of cultivation holes with the diameter of 1-1.5 cm on the top of the mixed cultivation material in the cultivation bottle body 1, injecting needle mushroom strain liquid into the two groups of cultivation holes in minutes, wherein one part of the needle mushroom strain liquid is left on the top surface of the mixed cultivation material, and the other part of the needle mushroom strain liquid flows into the bottom of the mixed cultivation material along the cultivation holes to finish cultivation, and in the inoculation process, as the diameters of the grid holes 3 are smaller, hypha and bacterial liquid are blocked by grids and are kept at the bottom of the mixed cultivation material and cannot flow into the bottle bottom;
S5, cultivation and cultivation
Placing the culture flask body 1 subjected to cultivation in an environment with the temperature of 5-25 ℃ for standing cultivation;
s6 mycelium stimulation
When hypha in the culture bottle body 1 grows to 3-5 cm in height, a disinfected mycelium stimulation hook is used, the cleaning end of the mycelium stimulation hook needs to be bent to a 90-degree angle, inoculation blocks on the surface of the culture bottle body 1 and the hypha aged on the surface layer are scraped off, the inoculation blocks and the hypha are finished to be flat, and the hypha naturally grows.
The experiment of the invention proves that:
3 the cultivation bottle of the invention and the ordinary cultivation bottle are filled with the same formulation, the cultivation material with the water content of 68.16% 1115g, the water content of the cultivation material at the lower part after bottling, and the water content at the upper part and the lower part of the sterilized, mycelium stimulation and collected waste material are as follows, and it can be seen that the water content of the bottle of the invention is not obviously changed because the water content at the bottom is slightly increased due to the punching and extrusion of the bottling after bottling. The water content of the upper part of the sterilized cultivation material has no obvious difference, but the water content of the lower part of the cultivation material is obviously higher and slightly accumulated in the lower part of a common bottle. From the index of the collected waste, the water under the grids can be slowly evaporated and absorbed by the cultivation material, so that the water content of the collected waste is higher than that of the common cultivation bottle, and the water supply of the needle mushrooms in the later growth stage is guaranteed.
Fruiting experiment data table:
and (4) conclusion: from the data, the same strains and formulas can ensure that the whiteness of the root of the product is better after the cultivation bottle disclosed by the invention is used, the root yellowing caused by water shortage is reduced, and the overall activity of the mushroom body is better.
Although embodiments of the present invention have been shown and described, it will be appreciated by those skilled in the art that changes, modifications, substitutions and alterations can be made in these embodiments without departing from the principles and spirit of the invention, the scope of which is defined in the appended claims and their equivalents.
Claims (8)
1. Novel edible mushroom cultivation bottle, including blake bottle body (1), its characterized in that: lower part fixed connection divider disc (2) in blake bottle body (1) inside wall, the inside latticed net hole (3) of arranging that is provided with of divider disc (2), blake bottle body (1) top delivery outlet fixed connection fixed cover ring (4), fixed cover ring (4) top delivery outlet is provided with sealed lid (5), the aperture of latticed hole (3) is 1.5 millimeters, 1 centimetre department above the diapire of inside of blake bottle body (1) is fixed in divider disc (2).
2. The use method of the novel edible mushroom cultivation bottle according to claim 1, characterized in that: the method comprises the following steps:
S1, stirring raw materials
Placing planting and cultivating raw materials of edible fungi such as needle mushrooms into a culture medium, correspondingly adding water into the culture medium, and stirring and mixing at a rotating speed of 20-30 revolutions per minute to form a mixed cultivation material;
s2, bottling
After being stirred uniformly, the mixed cultivation material is put into a culture flask body (1) and is carried by a separation disc (2);
s3, high-temperature sterilization
Sterilizing the culture bottle body (1) carrying the mixed culture material at high temperature, and heating the culture bottle body (1) for 1-1.5 hours at 80-95 ℃;
s4 inoculation
Punching two groups of cultivation holes with the diameter of 1-1.5 cm on the top of the mixed cultivation material in the cultivation bottle body (1), and injecting the flammulina velutipes strain liquid into the two groups of cultivation holes for minutes to finish cultivation;
s5, cultivation
Placing the culture flask body (1) which is subjected to cultivation in an environment with the temperature of 5-25 ℃ for standing cultivation;
s6 mycelium stimulation
When hyphae in the culture bottle body (1) grow to 3-5 cm, removing the inoculation blocks on the surface of the culture bottle body (1) and the hyphae aged on the surface layer by using a sterilized mycelium scratching hook, finishing and leveling, and allowing the hyphae to naturally grow.
3. The use method of the novel edible mushroom cultivation bottle according to claim 2, characterized in that: in the step of stirring the raw materials of S1, water is continuously added in the stirring process to ensure that the water content of the culture medium is 60-70%.
4. The use method of the novel edible fungus cultivation bottle as claimed in claim 2, wherein: in the step S2 of bottling, the mixed cultivation material is kept at an interval of 1 cm with the cultivation bottle body (1) under the load of the separation disc (2).
5. The use method of the novel edible fungus cultivation bottle as claimed in claim 2, wherein: in the step S4, a part of the flammulina velutipes strain liquid remains on the top surface of the mixed cultivation material, and a part of the flammulina velutipes strain liquid flows into the bottom of the mixed cultivation material along the cultivation holes.
6. The use method of the novel edible fungus cultivation bottle as claimed in claim 2, wherein: and in the step S3, in the sterilization process, if water enters, the water flows down along the grid holes (3) and is filtered to the bottom of the culture bottle body (1) through the grid, and the accumulated water cannot directly soak the culture material.
7. The use method of the novel edible fungus cultivation bottle as claimed in claim 2, wherein: in the step S4, in the inoculation process, hypha and bacterial liquid are blocked by the grids due to the small diameter of the grid holes (3) and are retained at the bottom of the mixed cultivation material and cannot flow into the bottle bottom.
8. The use method of the novel edible fungus cultivation bottle as claimed in claim 2, wherein: in the step S6, the cleaning end of the mycelium stimulation hook needs to be bent to form an angle of 90 degrees.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202210259644.7A CN114667891A (en) | 2022-03-16 | 2022-03-16 | Novel edible mushroom cultivation bottle and use method thereof |
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| Application Number | Priority Date | Filing Date | Title |
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| CN202210259644.7A CN114667891A (en) | 2022-03-16 | 2022-03-16 | Novel edible mushroom cultivation bottle and use method thereof |
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| CN114667891A true CN114667891A (en) | 2022-06-28 |
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| Application Number | Title | Priority Date | Filing Date |
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| CN202210259644.7A Pending CN114667891A (en) | 2022-03-16 | 2022-03-16 | Novel edible mushroom cultivation bottle and use method thereof |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN118525710A (en) * | 2024-05-20 | 2024-08-23 | 重庆华绿生物有限公司 | Multifunctional needle mushroom cultivation bottle |
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN118525710A (en) * | 2024-05-20 | 2024-08-23 | 重庆华绿生物有限公司 | Multifunctional needle mushroom cultivation bottle |
| CN118525710B (en) * | 2024-05-20 | 2025-04-04 | 重庆华绿生物有限公司 | A multifunctional Flammulina velutipes cultivation bottle |
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Application publication date: 20220628 |