CN114634535A - Separation and purification method of anoectochilus formosanus glycoside and application of anoectochilus formosanus glycoside in soothing cosmetics - Google Patents
Separation and purification method of anoectochilus formosanus glycoside and application of anoectochilus formosanus glycoside in soothing cosmetics Download PDFInfo
- Publication number
- CN114634535A CN114634535A CN202111561937.2A CN202111561937A CN114634535A CN 114634535 A CN114634535 A CN 114634535A CN 202111561937 A CN202111561937 A CN 202111561937A CN 114634535 A CN114634535 A CN 114634535A
- Authority
- CN
- China
- Prior art keywords
- glycoside
- anoectochilus
- extract
- filtrate
- anoectochilus formosanus
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 150000002338 glycosides Chemical class 0.000 title claims abstract description 40
- 229930182470 glycoside Natural products 0.000 title claims abstract description 39
- 238000000034 method Methods 0.000 title claims abstract description 33
- 239000002537 cosmetic Substances 0.000 title claims abstract description 14
- 238000000746 purification Methods 0.000 title claims abstract description 10
- 238000000926 separation method Methods 0.000 title claims abstract description 10
- 241000719836 Anoectochilus formosanus Species 0.000 title claims description 33
- 239000000284 extract Substances 0.000 claims abstract description 32
- 230000000694 effects Effects 0.000 claims abstract description 14
- 239000000706 filtrate Substances 0.000 claims description 32
- XEKOWRVHYACXOJ-UHFFFAOYSA-N Ethyl acetate Chemical compound CCOC(C)=O XEKOWRVHYACXOJ-UHFFFAOYSA-N 0.000 claims description 21
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 claims description 19
- 238000001914 filtration Methods 0.000 claims description 18
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 15
- NWUYHJFMYQTDRP-UHFFFAOYSA-N 1,2-bis(ethenyl)benzene;1-ethenyl-2-ethylbenzene;styrene Chemical compound C=CC1=CC=CC=C1.CCC1=CC=CC=C1C=C.C=CC1=CC=CC=C1C=C NWUYHJFMYQTDRP-UHFFFAOYSA-N 0.000 claims description 14
- 238000004537 pulping Methods 0.000 claims description 13
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 claims description 12
- 239000003456 ion exchange resin Substances 0.000 claims description 12
- 229920003303 ion-exchange polymer Polymers 0.000 claims description 12
- 239000007788 liquid Substances 0.000 claims description 12
- 238000002156 mixing Methods 0.000 claims description 10
- 239000006228 supernatant Substances 0.000 claims description 10
- 239000012466 permeate Substances 0.000 claims description 9
- 241000719837 Anoectochilus Species 0.000 claims description 8
- 238000000605 extraction Methods 0.000 claims description 8
- VYPSYNLAJGMNEJ-UHFFFAOYSA-N Silicium dioxide Chemical compound O=[Si]=O VYPSYNLAJGMNEJ-UHFFFAOYSA-N 0.000 claims description 7
- 239000000203 mixture Substances 0.000 claims description 5
- 239000002002 slurry Substances 0.000 claims description 5
- 238000005406 washing Methods 0.000 claims description 5
- 239000012535 impurity Substances 0.000 claims description 4
- 238000001035 drying Methods 0.000 claims description 3
- 239000003729 cation exchange resin Substances 0.000 claims description 2
- 229930185474 acteoside Natural products 0.000 claims 4
- FBSKJMQYURKNSU-ZLSOWSIRSA-N acteoside Chemical compound O[C@@H]1[C@H](O)[C@@H](O)[C@H](C)O[C@H]1O[C@H]1[C@H](OC(=O)\C=C\C=2C=C(O)C(O)=CC=2)[C@@H](CO)O[C@@H](OCCC=2C=C(O)C(O)=CC=2)[C@@H]1O FBSKJMQYURKNSU-ZLSOWSIRSA-N 0.000 claims 4
- FBSKJMQYURKNSU-UKQWSTALSA-N acteoside I Natural products C[C@@H]1O[C@H](O[C@@H]2[C@@H](O)[C@H](OCCc3ccc(O)c(O)c3)O[C@H](CO)[C@H]2OC(=O)C=Cc4ccc(O)c(O)c4)[C@H](O)[C@H](O)[C@H]1O FBSKJMQYURKNSU-UKQWSTALSA-N 0.000 claims 4
- QFRYQWYZSQDFOS-UHFFFAOYSA-N verbascoside Natural products CC1OC(COC2C(O)C(COC3OC(C(O)C(O)C3O)C(=O)O)OC(Oc4cc(O)cc5OC(=CC(=O)c45)c6ccc(O)c(O)c6)C2O)C(O)C(O)C1O QFRYQWYZSQDFOS-UHFFFAOYSA-N 0.000 claims 4
- 241000934230 Anoectochilus roxburghii Species 0.000 abstract description 29
- 210000004027 cell Anatomy 0.000 abstract description 11
- YKPUWZUDDOIDPM-SOFGYWHQSA-N capsaicin Chemical compound COC1=CC(CNC(=O)CCCC\C=C\C(C)C)=CC=C1O YKPUWZUDDOIDPM-SOFGYWHQSA-N 0.000 abstract description 10
- 102000003566 TRPV1 Human genes 0.000 abstract description 9
- 101150016206 Trpv1 gene Proteins 0.000 abstract description 9
- BHPQYMZQTOCNFJ-UHFFFAOYSA-N Calcium cation Chemical compound [Ca+2] BHPQYMZQTOCNFJ-UHFFFAOYSA-N 0.000 abstract description 6
- 229910001424 calcium ion Inorganic materials 0.000 abstract description 6
- 230000004913 activation Effects 0.000 abstract description 5
- 229960002504 capsaicin Drugs 0.000 abstract description 5
- 235000017663 capsaicin Nutrition 0.000 abstract description 5
- 238000002360 preparation method Methods 0.000 abstract description 5
- 230000002757 inflammatory effect Effects 0.000 abstract description 4
- 230000002401 inhibitory effect Effects 0.000 abstract description 4
- 210000003292 kidney cell Anatomy 0.000 abstract description 3
- 230000000638 stimulation Effects 0.000 abstract description 3
- 230000000977 initiatory effect Effects 0.000 abstract 1
- 230000037307 sensitive skin Effects 0.000 description 8
- 239000000243 solution Substances 0.000 description 8
- 206010061218 Inflammation Diseases 0.000 description 7
- 230000008569 process Effects 0.000 description 7
- 239000000523 sample Substances 0.000 description 7
- 210000003491 skin Anatomy 0.000 description 7
- PEDCQBHIVMGVHV-UHFFFAOYSA-N Glycerine Chemical compound OCC(O)CO PEDCQBHIVMGVHV-UHFFFAOYSA-N 0.000 description 6
- 230000004054 inflammatory process Effects 0.000 description 6
- 239000003814 drug Substances 0.000 description 5
- 239000000049 pigment Substances 0.000 description 5
- PUPZLCDOIYMWBV-UHFFFAOYSA-N (+/-)-1,3-Butanediol Chemical compound CC(O)CCO PUPZLCDOIYMWBV-UHFFFAOYSA-N 0.000 description 4
- TXFPEBPIARQUIG-UHFFFAOYSA-N 4'-hydroxyacetophenone Chemical compound CC(=O)C1=CC=C(O)C=C1 TXFPEBPIARQUIG-UHFFFAOYSA-N 0.000 description 4
- 241000196324 Embryophyta Species 0.000 description 4
- 235000019441 ethanol Nutrition 0.000 description 4
- 230000004941 influx Effects 0.000 description 4
- 210000002540 macrophage Anatomy 0.000 description 4
- 208000024891 symptom Diseases 0.000 description 4
- WEVYAHXRMPXWCK-UHFFFAOYSA-N Acetonitrile Chemical compound CC#N WEVYAHXRMPXWCK-UHFFFAOYSA-N 0.000 description 3
- 206010015150 Erythema Diseases 0.000 description 3
- OKKJLVBELUTLKV-UHFFFAOYSA-N Methanol Chemical compound OC OKKJLVBELUTLKV-UHFFFAOYSA-N 0.000 description 3
- 208000003251 Pruritus Diseases 0.000 description 3
- 239000002253 acid Substances 0.000 description 3
- 239000007853 buffer solution Substances 0.000 description 3
- 239000003795 chemical substances by application Substances 0.000 description 3
- 238000001514 detection method Methods 0.000 description 3
- 229930003944 flavone Natural products 0.000 description 3
- 235000011949 flavones Nutrition 0.000 description 3
- 239000002994 raw material Substances 0.000 description 3
- 238000011160 research Methods 0.000 description 3
- HEDRZPFGACZZDS-UHFFFAOYSA-N Chloroform Chemical compound ClC(Cl)Cl HEDRZPFGACZZDS-UHFFFAOYSA-N 0.000 description 2
- OMFXVFTZEKFJBZ-UHFFFAOYSA-N Corticosterone Natural products O=C1CCC2(C)C3C(O)CC(C)(C(CC4)C(=O)CO)C4C3CCC2=C1 OMFXVFTZEKFJBZ-UHFFFAOYSA-N 0.000 description 2
- 206010020751 Hypersensitivity Diseases 0.000 description 2
- XUMBMVFBXHLACL-UHFFFAOYSA-N Melanin Chemical compound O=C1C(=O)C(C2=CNC3=C(C(C(=O)C4=C32)=O)C)=C2C4=CNC2=C1C XUMBMVFBXHLACL-UHFFFAOYSA-N 0.000 description 2
- 241000699670 Mus sp. Species 0.000 description 2
- MWUXSHHQAYIFBG-UHFFFAOYSA-N Nitric oxide Chemical compound O=[N] MWUXSHHQAYIFBG-UHFFFAOYSA-N 0.000 description 2
- 241000233855 Orchidaceae Species 0.000 description 2
- NBIIXXVUZAFLBC-UHFFFAOYSA-N Phosphoric acid Chemical compound OP(O)(O)=O NBIIXXVUZAFLBC-UHFFFAOYSA-N 0.000 description 2
- GAMYVSCDDLXAQW-AOIWZFSPSA-N Thermopsosid Natural products O(C)c1c(O)ccc(C=2Oc3c(c(O)cc(O[C@H]4[C@H](O)[C@@H](O)[C@H](O)[C@H](CO)O4)c3)C(=O)C=2)c1 GAMYVSCDDLXAQW-AOIWZFSPSA-N 0.000 description 2
- 208000026935 allergic disease Diseases 0.000 description 2
- 230000003110 anti-inflammatory effect Effects 0.000 description 2
- 235000019437 butane-1,3-diol Nutrition 0.000 description 2
- 238000004140 cleaning Methods 0.000 description 2
- OMFXVFTZEKFJBZ-HJTSIMOOSA-N corticosterone Chemical compound O=C1CC[C@]2(C)[C@H]3[C@@H](O)C[C@](C)([C@H](CC4)C(=O)CO)[C@@H]4[C@@H]3CCC2=C1 OMFXVFTZEKFJBZ-HJTSIMOOSA-N 0.000 description 2
- 238000012258 culturing Methods 0.000 description 2
- 229940079593 drug Drugs 0.000 description 2
- 238000010828 elution Methods 0.000 description 2
- 231100000321 erythema Toxicity 0.000 description 2
- 150000002212 flavone derivatives Chemical class 0.000 description 2
- 235000013305 food Nutrition 0.000 description 2
- JYGXADMDTFJGBT-VWUMJDOOSA-N hydrocortisone Chemical compound O=C1CC[C@]2(C)[C@H]3[C@@H](O)C[C@](C)([C@@](CC4)(O)C(=O)CO)[C@@H]4[C@@H]3CCC2=C1 JYGXADMDTFJGBT-VWUMJDOOSA-N 0.000 description 2
- 230000028993 immune response Effects 0.000 description 2
- 238000011534 incubation Methods 0.000 description 2
- 239000004615 ingredient Substances 0.000 description 2
- 230000005764 inhibitory process Effects 0.000 description 2
- 230000007803 itching Effects 0.000 description 2
- 210000002510 keratinocyte Anatomy 0.000 description 2
- 238000002386 leaching Methods 0.000 description 2
- 210000004185 liver Anatomy 0.000 description 2
- 239000000463 material Substances 0.000 description 2
- 230000004048 modification Effects 0.000 description 2
- 238000012986 modification Methods 0.000 description 2
- 210000005036 nerve Anatomy 0.000 description 2
- 125000002801 octanoyl group Chemical group C(CCCCCCC)(=O)* 0.000 description 2
- 230000001575 pathological effect Effects 0.000 description 2
- 239000003755 preservative agent Substances 0.000 description 2
- 102000005962 receptors Human genes 0.000 description 2
- 108020003175 receptors Proteins 0.000 description 2
- 230000001105 regulatory effect Effects 0.000 description 2
- 150000003839 salts Chemical class 0.000 description 2
- 239000000741 silica gel Substances 0.000 description 2
- 229910002027 silica gel Inorganic materials 0.000 description 2
- 239000007787 solid Substances 0.000 description 2
- 239000002904 solvent Substances 0.000 description 2
- 230000035882 stress Effects 0.000 description 2
- 210000001519 tissue Anatomy 0.000 description 2
- 230000001960 triggered effect Effects 0.000 description 2
- VHBFFQKBGNRLFZ-UHFFFAOYSA-N vitamin p Natural products O1C2=CC=CC=C2C(=O)C=C1C1=CC=CC=C1 VHBFFQKBGNRLFZ-UHFFFAOYSA-N 0.000 description 2
- KIUKXJAPPMFGSW-YXBJCWEESA-N (2s,4s,5r,6s)-6-[(2s,3r,5s,6r)-3-acetamido-2-[(3s,4r,5r,6r)-6-[(3r,4r,5s,6r)-3-acetamido-2,5-dihydroxy-6-(hydroxymethyl)oxan-4-yl]oxy-2-carboxy-4,5-dihydroxyoxan-3-yl]oxy-5-hydroxy-6-(hydroxymethyl)oxan-4-yl]oxy-3,4,5-trihydroxyoxane-2-carboxylic acid Chemical compound CC(=O)N[C@H]1C(O)O[C@H](CO)[C@@H](O)[C@@H]1O[C@H]1[C@H](O)[C@@H](O)[C@H](O[C@H]2[C@@H](C(O[C@@H]3[C@@H]([C@@H](O)C(O)[C@H](O3)C(O)=O)O)[C@H](O)[C@@H](CO)O2)NC(C)=O)C(C(O)=O)O1 KIUKXJAPPMFGSW-YXBJCWEESA-N 0.000 description 1
- MQEPWBMWFIVRPS-ZGSHZZHUSA-N (3R)-5-Oxotetrahydro-3-furanyl beta-D-glucopyranoside Chemical compound O[C@@H]1[C@@H](O)[C@H](O)[C@@H](CO)O[C@H]1O[C@@H]1CC(=O)OC1 MQEPWBMWFIVRPS-ZGSHZZHUSA-N 0.000 description 1
- FUFLCEKSBBHCMO-UHFFFAOYSA-N 11-dehydrocorticosterone Natural products O=C1CCC2(C)C3C(=O)CC(C)(C(CC4)C(=O)CO)C4C3CCC2=C1 FUFLCEKSBBHCMO-UHFFFAOYSA-N 0.000 description 1
- HIXDQWDOVZUNNA-UHFFFAOYSA-N 2-(3,4-dimethoxyphenyl)-5-hydroxy-7-methoxychromen-4-one Chemical compound C=1C(OC)=CC(O)=C(C(C=2)=O)C=1OC=2C1=CC=C(OC)C(OC)=C1 HIXDQWDOVZUNNA-UHFFFAOYSA-N 0.000 description 1
- UXVMQQNJUSDDNG-UHFFFAOYSA-L Calcium chloride Chemical compound [Cl-].[Cl-].[Ca+2] UXVMQQNJUSDDNG-UHFFFAOYSA-L 0.000 description 1
- 244000247747 Coptis groenlandica Species 0.000 description 1
- 235000002991 Coptis groenlandica Nutrition 0.000 description 1
- MFYSYFVPBJMHGN-ZPOLXVRWSA-N Cortisone Chemical compound O=C1CC[C@]2(C)[C@H]3C(=O)C[C@](C)([C@@](CC4)(O)C(=O)CO)[C@@H]4[C@@H]3CCC2=C1 MFYSYFVPBJMHGN-ZPOLXVRWSA-N 0.000 description 1
- MFYSYFVPBJMHGN-UHFFFAOYSA-N Cortisone Natural products O=C1CCC2(C)C3C(=O)CC(C)(C(CC4)(O)C(=O)CO)C4C3CCC2=C1 MFYSYFVPBJMHGN-UHFFFAOYSA-N 0.000 description 1
- 201000004624 Dermatitis Diseases 0.000 description 1
- 239000006144 Dulbecco’s modified Eagle's medium Substances 0.000 description 1
- 102000004190 Enzymes Human genes 0.000 description 1
- 108090000790 Enzymes Proteins 0.000 description 1
- 201000001431 Hyperuricemia Diseases 0.000 description 1
- 240000002853 Nelumbo nucifera Species 0.000 description 1
- 235000006508 Nelumbo nucifera Nutrition 0.000 description 1
- 235000006510 Nelumbo pentapetala Nutrition 0.000 description 1
- 208000007920 Neurogenic Inflammation Diseases 0.000 description 1
- 206010040844 Skin exfoliation Diseases 0.000 description 1
- 229920002385 Sodium hyaluronate Polymers 0.000 description 1
- 210000001744 T-lymphocyte Anatomy 0.000 description 1
- 206010043189 Telangiectasia Diseases 0.000 description 1
- 102000003425 Tyrosinase Human genes 0.000 description 1
- 108060008724 Tyrosinase Proteins 0.000 description 1
- 102100033220 Xanthine oxidase Human genes 0.000 description 1
- 108010093894 Xanthine oxidase Proteins 0.000 description 1
- 230000009471 action Effects 0.000 description 1
- 239000004480 active ingredient Substances 0.000 description 1
- 239000011149 active material Substances 0.000 description 1
- 230000032683 aging Effects 0.000 description 1
- 230000007815 allergy Effects 0.000 description 1
- 229910000147 aluminium phosphate Inorganic materials 0.000 description 1
- 230000003712 anti-aging effect Effects 0.000 description 1
- 230000003266 anti-allergic effect Effects 0.000 description 1
- 230000003078 antioxidant effect Effects 0.000 description 1
- 210000003719 b-lymphocyte Anatomy 0.000 description 1
- 238000010923 batch production Methods 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 230000015572 biosynthetic process Effects 0.000 description 1
- 210000004369 blood Anatomy 0.000 description 1
- 239000008280 blood Substances 0.000 description 1
- 239000001110 calcium chloride Substances 0.000 description 1
- 235000011148 calcium chloride Nutrition 0.000 description 1
- 229910001628 calcium chloride Inorganic materials 0.000 description 1
- 230000001914 calming effect Effects 0.000 description 1
- 150000001720 carbohydrates Chemical class 0.000 description 1
- 238000006243 chemical reaction Methods 0.000 description 1
- 238000004587 chromatography analysis Methods 0.000 description 1
- 239000013068 control sample Substances 0.000 description 1
- 238000001816 cooling Methods 0.000 description 1
- 229960004544 cortisone Drugs 0.000 description 1
- 230000035618 desquamation Effects 0.000 description 1
- 238000007865 diluting Methods 0.000 description 1
- 239000003596 drug target Substances 0.000 description 1
- 239000000469 ethanolic extract Substances 0.000 description 1
- RTZKZFJDLAIYFH-UHFFFAOYSA-N ether Substances CCOCC RTZKZFJDLAIYFH-UHFFFAOYSA-N 0.000 description 1
- 150000002213 flavones Chemical class 0.000 description 1
- 239000000499 gel Substances 0.000 description 1
- 239000003862 glucocorticoid Substances 0.000 description 1
- 150000004676 glycans Chemical class 0.000 description 1
- MQEPWBMWFIVRPS-UHFFFAOYSA-N goodyeroside A Natural products OC1C(O)C(O)C(CO)OC1OC1CC(=O)OC1 MQEPWBMWFIVRPS-UHFFFAOYSA-N 0.000 description 1
- 235000013402 health food Nutrition 0.000 description 1
- 238000004128 high performance liquid chromatography Methods 0.000 description 1
- 210000003630 histaminocyte Anatomy 0.000 description 1
- 229960000890 hydrocortisone Drugs 0.000 description 1
- 238000003384 imaging method Methods 0.000 description 1
- 230000001900 immune effect Effects 0.000 description 1
- 230000008105 immune reaction Effects 0.000 description 1
- 210000000987 immune system Anatomy 0.000 description 1
- 230000036039 immunity Effects 0.000 description 1
- 208000027866 inflammatory disease Diseases 0.000 description 1
- 239000007924 injection Substances 0.000 description 1
- 238000002347 injection Methods 0.000 description 1
- 229910052500 inorganic mineral Inorganic materials 0.000 description 1
- 238000002955 isolation Methods 0.000 description 1
- 150000002596 lactones Chemical group 0.000 description 1
- 235000013372 meat Nutrition 0.000 description 1
- 230000007246 mechanism Effects 0.000 description 1
- 230000003340 mental effect Effects 0.000 description 1
- 238000003808 methanol extraction Methods 0.000 description 1
- 244000000010 microbial pathogen Species 0.000 description 1
- 239000012982 microporous membrane Substances 0.000 description 1
- 239000011707 mineral Substances 0.000 description 1
- 235000010755 mineral Nutrition 0.000 description 1
- 239000011259 mixed solution Substances 0.000 description 1
- 210000001640 nerve ending Anatomy 0.000 description 1
- 208000035824 paresthesia Diseases 0.000 description 1
- 239000003208 petroleum Substances 0.000 description 1
- 239000002504 physiological saline solution Substances 0.000 description 1
- 230000009894 physiological stress Effects 0.000 description 1
- 229920001282 polysaccharide Polymers 0.000 description 1
- 239000005017 polysaccharide Substances 0.000 description 1
- 230000002335 preservative effect Effects 0.000 description 1
- 102000004169 proteins and genes Human genes 0.000 description 1
- 108090000623 proteins and genes Proteins 0.000 description 1
- YQUVCSBJEUQKSH-UHFFFAOYSA-N protochatechuic acid Natural products OC(=O)C1=CC=C(O)C(O)=C1 YQUVCSBJEUQKSH-UHFFFAOYSA-N 0.000 description 1
- 230000008439 repair process Effects 0.000 description 1
- 230000004044 response Effects 0.000 description 1
- 238000013341 scale-up Methods 0.000 description 1
- 238000012216 screening Methods 0.000 description 1
- 230000028327 secretion Effects 0.000 description 1
- 230000035945 sensitivity Effects 0.000 description 1
- 210000001044 sensory neuron Anatomy 0.000 description 1
- 210000004911 serous fluid Anatomy 0.000 description 1
- 210000002966 serum Anatomy 0.000 description 1
- 230000009759 skin aging Effects 0.000 description 1
- 230000008591 skin barrier function Effects 0.000 description 1
- 229940010747 sodium hyaluronate Drugs 0.000 description 1
- YWIVKILSMZOHHF-QJZPQSOGSA-N sodium;(2s,3s,4s,5r,6r)-6-[(2s,3r,4r,5s,6r)-3-acetamido-2-[(2s,3s,4r,5r,6r)-6-[(2r,3r,4r,5s,6r)-3-acetamido-2,5-dihydroxy-6-(hydroxymethyl)oxan-4-yl]oxy-2-carboxy-4,5-dihydroxyoxan-3-yl]oxy-5-hydroxy-6-(hydroxymethyl)oxan-4-yl]oxy-3,4,5-trihydroxyoxane-2- Chemical compound [Na+].CC(=O)N[C@H]1[C@H](O)O[C@H](CO)[C@@H](O)[C@@H]1O[C@H]1[C@H](O)[C@@H](O)[C@H](O[C@H]2[C@@H]([C@@H](O[C@H]3[C@@H]([C@@H](O)[C@H](O)[C@H](O3)C(O)=O)O)[C@H](O)[C@@H](CO)O2)NC(C)=O)[C@@H](C(O)=O)O1 YWIVKILSMZOHHF-QJZPQSOGSA-N 0.000 description 1
- 238000003756 stirring Methods 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
- 235000019605 sweet taste sensations Nutrition 0.000 description 1
- 238000003786 synthesis reaction Methods 0.000 description 1
- 208000009056 telangiectasis Diseases 0.000 description 1
- 238000012360 testing method Methods 0.000 description 1
- 239000002562 thickening agent Substances 0.000 description 1
- 231100000331 toxic Toxicity 0.000 description 1
- 230000002588 toxic effect Effects 0.000 description 1
- 230000009466 transformation Effects 0.000 description 1
- 230000001052 transient effect Effects 0.000 description 1
- WKOLLVMJNQIZCI-UHFFFAOYSA-N vanillic acid Chemical compound COC1=CC(C(O)=O)=CC=C1O WKOLLVMJNQIZCI-UHFFFAOYSA-N 0.000 description 1
- TUUBOHWZSQXCSW-UHFFFAOYSA-N vanillic acid Natural products COC1=CC(O)=CC(C(O)=O)=C1 TUUBOHWZSQXCSW-UHFFFAOYSA-N 0.000 description 1
- 238000003809 water extraction Methods 0.000 description 1
- 230000002087 whitening effect Effects 0.000 description 1
- 239000012224 working solution Substances 0.000 description 1
Images
Classifications
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
- C07H1/00—Processes for the preparation of sugar derivatives
- C07H1/06—Separation; Purification
- C07H1/08—Separation; Purification from natural products
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/30—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds
- A61K8/60—Sugars; Derivatives thereof
- A61K8/602—Glycosides, e.g. rutin
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P29/00—Non-central analgesic, antipyretic or antiinflammatory agents, e.g. antirheumatic agents; Non-steroidal antiinflammatory drugs [NSAID]
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q19/00—Preparations for care of the skin
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
- C07H17/00—Compounds containing heterocyclic radicals directly attached to hetero atoms of saccharide radicals
- C07H17/04—Heterocyclic radicals containing only oxygen as ring hetero atoms
Landscapes
- Health & Medical Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Organic Chemistry (AREA)
- General Health & Medical Sciences (AREA)
- Animal Behavior & Ethology (AREA)
- Public Health (AREA)
- Veterinary Medicine (AREA)
- Biotechnology (AREA)
- Genetics & Genomics (AREA)
- Engineering & Computer Science (AREA)
- Biochemistry (AREA)
- Molecular Biology (AREA)
- Rheumatology (AREA)
- Birds (AREA)
- Dermatology (AREA)
- Pain & Pain Management (AREA)
- Epidemiology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- General Chemical & Material Sciences (AREA)
- Medicinal Chemistry (AREA)
- Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
- Pharmacology & Pharmacy (AREA)
- Medicines Containing Plant Substances (AREA)
- Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
Abstract
A separation and purification method of Anoectochilus roxburghii glycoside and its application in soothing cosmetics are provided, wherein an Anoectochilus roxburghii extract capable of inhibiting NO release is screened from Anoectochilus roxburghii by adopting RAW264.7 cell model, the main component is Anoectochilus roxburghii glycoside, and the preparation method of the extract is designed and optimized; meanwhile, the extract can inhibit the activation of TRPV1 on human embryonic kidney cells caused by capsaicin stimulation, reduce the calcium ion inflow of cells caused by inflammatory mediators, avoid the initiation of corresponding physiological changes and the like, and further prove that the extract has the effect of relieving.
Description
Technical Field
The invention belongs to the technical field of extraction, separation, transformation and application of natural plant active ingredients, and particularly relates to a preparation method and application of an anoectochilus formosanus extract with a relieving effect.
Background
Anoectochilus roxburghii is a common name of Anoectochilus roxburghii, is a plant of Orchidaceae (Orchidaceae) Kaliptae (Anoectochilus), and more than 17 plants of the genus are currently found in China. Anoectochilus roxburghii is produced in Fujian province and is a genuine herb in Fujian province.
According to records in Xinhua Bencao gang Yao, the whole herb of Anoectochilus roxburghii can be used as a medicine, has sweet taste and mild nature, and has the effects of cooling blood, calming liver, and clearing heat and detoxicating.
At present, anoectochilus roxburghii has been approved as food by the national food and drug administration, so that the anoectochilus roxburghii has a wide market in the fields of food and health food. However, the application and research of anoectochilus formosanus in the field of cosmetics are relatively few. Chinese patent CN2017106813818 adopts water extraction, alcohol extraction and other methods to obtain anoectochilus roxburghii extract, and the extract can inhibit melanin and can be used as a raw material for whitening cosmetics. CN2016107666883 proposes a separation and purification process of herba Anoectochili Roxburghii flavone, and indicates that the flavone has antioxidant activity and tyrosinase activity inhibiting effect.
Sensitive skin (sensitive skin) refers to a high-response state of skin under physiological or pathological conditions, which may show subjective symptoms such as burning, stabbing pain, itching and tightness, with or without objective signs such as erythema, telangiectasia and desquamation. In recent years, as people attach more and more importance to the appearance, the cleaning and care of the skin of the people are intentionally enhanced, but the excessive cleaning of the skin and the improper use of cosmetics easily induce the sensitive skin, and the incidence rate of the sensitive skin tends to increase. In addition, physiological stress such as overtime and mental stress can increase secretion of corticosterone, and the corticosterone is converted into cortisol (endogenous glucocorticoid) by enzymes in skin tissues, and the cortisone can reduce skin inflammation in a short period of time, but also reduce skin immunity and repair capacity, so that sensitive skin is developed. [i]
The domestic and foreign researches have considered that after the sensitive skin is generated and the skin barrier is damaged, the nerve endings are stimulated by the outside, the transient receptor potential vanillic acid subtype 1(TRPV1) distributed in the sensory nerve endings, keratinocytes and mast cells expresses some receptors capable of sensing temperature, pain and the like on the surfaces of the keratinocytes, such as TRPs. For example, TRPV1 and TRPV1 trigger nerve reactions in two ways, on one hand, electrical signals are transmitted to nerve centers to generate symptoms such as tingling and itching, and on the other hand, calcium ion influx triggered by TRPV1 activation can trigger neurogenic inflammation, and finally, symptoms such as skin redness and erythema are caused. [ ii ] therefore, TRPV1 can be used for evaluating a cosmetic raw material having soothing efficacy.
In addition, sensitive skin is also an inflammatory reaction in nature, and NO plays a key regulatory role in inflammation and immune response. In the immune system, the synthesis of NO is an important mark for macrophage activation and is an important step for macrophage to generate pathological and immune effects, human body macrophages can generate NO under the condition of inflammatory diseases, inhibit or kill pathogenic microorganisms, and meanwhile, inflammatory immune reaction can be carried out under the participation of B lymphocytes and T lymphocytes, so that a sequence of physiological symptoms are triggered. Therefore, inhibition of NO release is commonly used to screen and evaluate anti-inflammatory and anti-allergic active materials.
The content of the anoectochilus formosanus glycoside in the anoectochilus formosanus is high (up to 25 percent), and the anoectochilus formosanus glycoside is easy to dissolve in water and is very convenient to extract. However, Anoectochilus roxburghii also contains flavones, pigments, inorganic salts, plant polysaccharides and the like, wherein the pigments and the inorganic salts influence the use of the Anoectochilus roxburghii in cosmetics. In addition, the roxburgh anoectochilus terminal bud glycoside has a fragile lactone ring structure and a fragile C-O glycoside structure, and can be easily hydrolyzed at high temperature or under the conditions of strong acid and strong base, so that the extraction and purification process parameters of the roxburgh anoectochilus terminal bud glycoside are limited to a certain extent.
Chinese patent 2015105110163 adopts petroleum ether extraction, methanol extraction, D101 crude separation, silica gel column subdivision, D101 purification and other processes to obtain the Anoectochilus roxburghii glycoside with purity of more than 95%, and the process is complex and uses methanol, chloroform and other toxic solvents.
Document [ iii ] discloses a method for separating off Anoectochilus roxburghii glycoside by using a combination of ODS and silica gel column, but this method is difficult to scale up and expensive in process cost.
Disclosure of Invention
Aiming at the problems, the invention provides a separation and purification method of anoectochilus formosanus glycoside and application of the anoectochilus formosanus glycoside in soothing cosmetics.
In order to realize the purpose of the invention, the invention adopts the following technical scheme:
a separation and purification method of anoectochilus formosanus glycoside comprises the following process steps:
(1) pulping: putting fresh anoectochilus formosanus and water into a pulping machine according to a proportion and pulping into slurry;
(2) coarse filtration: conveying the feed liquid to a bag filter for filtering, and collecting filtrate; washing the filter residue with water, and collecting the filtrate; mixing the two filtrates;
(3) fine filtering: fine filtering the filtrate with diatomite filter, and collecting fine filtrate;
(4) purifying: after the fine filtrate is pressurized by a pump, impurities in the feed liquid are removed through an activated carbon column and an ion exchange resin column in sequence, and the permeate is collected;
(5) concentration: concentrating the permeate at low temperature in vacuum to obtain extract;
(6) and (3) extraction: mixing the extract with an extractant, standing, and collecting supernatant;
(7) concentrating and drying: concentrating the supernatant under reduced pressure to obtain the anoectochilus formosanus glycoside.
Further, the ion exchange resin used is a cation exchange resin.
Further, the extracting agent is one or a mixture of ethyl acetate and absolute ethyl alcohol.
Further, the ion exchange resin used was LX-S1860 ion exchange resin.
Further, the extractant is a mixture of ethyl acetate and absolute ethyl alcohol, and the ratio of the absolute ethyl alcohol to the ethyl acetate is 2: 8.
The roxburgh anoectochilus terminal bud glycoside is added into the cosmetics with the relieving effect, and the addition amount is 0.01% -20%. The preferred addition amount is 2% to 5%.
By adopting the technical scheme, the invention has the following main innovation points and beneficial effects:
(1) by adopting the process, the extraction rate of the anoectochilus formosanus glycoside is more than 90%, the purity is more than 75%, and impurities such as pigment and the like are avoided due to the existing process. Anoectochilus formosanus glycoside is hydrophilic micromolecule, and has high solubility in water or absolute ethyl alcohol. According to the property, in the process, activated carbon is used for removing part of pigments and most of fat-soluble components, mineral substances and water-soluble pigments are removed through an ion exchange resin column, and finally, anhydrous ethanol is used for removing water-soluble saccharides and proteins.
(2) The process is simple, the equipment investment is low, and the method is suitable for batch production.
(3) The extract (Anoectochilus roxburghii glycoside) has soothing effect by activity screening and testing. NO plays a key regulatory role in inflammation and immune response, and the extract can inhibit NO release from macrophages stimulated by LPS, thereby inhibiting the progress of inflammatory reaction. Meanwhile, the extract can inhibit the activation of TRPV1 on human embryonic kidney cells caused by capsaicin stimulation, reduce the calcium ion influx of cells caused by inflammatory mediators, avoid the triggering of corresponding physiological changes (such as pain) and the like, and reduce the sensitivity of skin itch or pain sense.
Description of the drawings:
FIG. 1 shows a control sample of Anoectochilus roxburghii (leaf source organism, purity is more than or equal to 95%);
FIG. 2 is Anoectochilus roxburghii extract (example 1, purity 78%);
FIG. 3 is a screen of anti-inflammatory activity in Anoectochilus roxburghii (1. mu.g/mL);
FIG. 4 shows that Anoectochilus roxburghii extract (Anoectochilus roxburghii glycoside) can inhibit NO release;
FIG. 5 shows that Anoectochilus roxburghii extract (Anoectochilus roxburghii glycoside) can inhibit the influx of calcium ions into cells caused by capsaicin.
Detailed Description
The present invention is further illustrated by the accompanying fig. 1-5 and the detailed description, which describe only some embodiments of the invention and are not intended to limit the scope of the invention. Other embodiments, which can be made by persons skilled in the art without any inventive contribution, fall within the scope of protection of the present invention.
A separation and purification method of anoectochilus formosanus glycoside comprises the following process steps:
(1) pulping: putting fresh anoectochilus formosanus and water into a pulping machine according to a proportion and pulping into slurry;
(2) coarse filtration: conveying the feed liquid to a bag filter for filtering, and collecting filtrate; washing the filter residue with water, and collecting the filtrate; mixing the two filtrates;
(3) fine filtering: fine filtering the filtrate with diatomite filter, and collecting fine filtrate;
(4) purifying: after the fine filtrate is pressurized by a pump, impurities in the feed liquid are removed through an activated carbon column and an ion exchange resin column in sequence, and the permeate is collected;
(5) concentration: concentrating the permeate at low temperature in vacuum to obtain extract;
(6) and (3) extraction: mixing the extract with an extractant, standing, and collecting supernatant;
(7) concentrating and drying: concentrating the supernatant under reduced pressure to obtain the anoectochilus formosanus glycoside.
The following is further detailed in several groups of specific examples:
example 1:
(1) taking 1kg of fresh Anoectochilus roxburghii (Anoectochilus roxburghii glycoside content is 3.02%) and 3kg of water according to a certain proportion, and pulping into serous fluid in a pulping machine.
(2) Pouring the slurry into a bag filter, and collecting a crude filtrate; washing filter residues with 1-2 kg of pure water, and collecting a crude filtrate; the two crude filtrates were combined.
(3) Fine filtering the filtrate with diatomite filter, and collecting the fine filtrate.
(4) After the fine filtrate is pressurized by a pump, the fine filtrate sequentially passes through an activated carbon column and an LX-S1860 ion exchange resin column, and the permeate is collected.
(5) Concentrating the permeate at 50 deg.C and-0.095 MPa to obtain 0.104kg extract.
(6) And (3) fully mixing the extract with 500mL of absolute ethyl alcohol, and pouring out supernatant liquid, namely the extract liquid.
(7) Concentrating the extractive solution at 50 deg.C under-0.095 MPa to obtain light yellow solid 35.4g, which has a content of 78.4% and a total yield of 91.7% by HPLC.
Example 2:
(1) taking 10kg of fresh Anoectochilus roxburghii (Anoectochilus roxburghii glycoside content is 3.37%) and 25kg of water according to a certain proportion, and pulping into pulp in a pulping machine.
(2) Pouring the slurry into a bag filter, and collecting a crude filtrate; washing filter residues with 3-5 kg of pure water, and collecting a crude filtrate; the two crude filtrates were combined.
(3) Fine filtering the filtrate with diatomite filter, and collecting the fine filtrate.
(4) After the fine filtration liquid is pressurized by a pump, the fine filtration liquid sequentially passes through an activated carbon column and an LX-S1860 ion exchange resin column, and the permeation liquid is collected.
(5) The permeate was concentrated at 50 deg.C and-0.095 MPa to obtain 1.22kg extract.
(6) Mixing the extract with 3L of mixed solution (ethanol: ethyl acetate is 8:2), and pouring out supernatant to obtain extract; extracting the bottom extract once again; the two extracts were combined.
(7) Concentrating the extractive solution at 50 deg.C under-0.095 MPa to obtain white flake solid 372g with Anoectochilus formosanus glycoside content of 81.5% and Anoectochilus formosanus glycoside total yield of 92.7%.
Example 3:
a preparation method of essence with a relieving effect,
(1) the ingredients are as follows: water, glycerol, 1, 3-butanediol, anoectochilus formosanus glycoside, sodium hyaluronate, p-hydroxyacetophenone and caprylyl hydroximic acid. (except for the Anoectochilus formosanus glycoside, other raw materials can be purchased from the market.)
(2) The mixture ratio is as follows:
| ingredients | Proportioning | Remarks for note |
| Pure water | Balance of | Solvent(s) |
| Glycerol | 10.0％ | Moisture-retaining agent |
| 1, 3-butanediol | 5.0％ | Moisture-retaining agent |
| Anoectochilus formosanus glycoside | 2.5％ | Functional component |
| Hyaluronic acid sodium salt | 0.5％ | Thickening agent |
| P-hydroxyacetophenone | 0.3％ | Preservative |
| Octanoyl hydroximic acid | 0.1％ | Preservative agent |
(3) The preparation method comprises the following steps: mixing the above materials, and stirring to dissolve.
To demonstrate the effectiveness of the invention, supplementary explanations were made:
1. determination of Anoectochilus formosanus glycoside content
The method comprises the following steps:
crushing a gold thread lotus sample by using a meat grinder, putting 1-2 g of the sample into a beaker, adding 50% ethanol, leaching at 60 ℃, and filtering; leaching the filter residue for 2 times; mixing the filtrates, placing in a 250mL volumetric flask, diluting to constant volume with 50% ethanol, shaking, collecting a little solution, filtering with microporous membrane, placing the filtrate in a sample bottle, and analyzing the sample with an instrument.
The equipment configuration: chromatography column (Inertsil ODS-3, 5 μ M, 4.6X 250mm), detector (SPD-M20A), binary gradient pump (LC-16), column incubator (Essentia CTO-16).
Chromatographic conditions are as follows: the mobile phase A is acetonitrile, and the mobile phase B is 0.2% phosphoric acid water solution; the flow rate is 1.0mL/min, and the elution method is gradient elution (0-5min: 0% A; 5-10min: 0% A → 10% A); the column temperature is 30 ℃, the detection wavelength is 200nm, and the sample injection amount is 5 mu L.
As a result: FIGS. 1 to 2
2. Soothing effect
The method comprises the following steps: inoculating RAW264.7 cells into a 48-well plate according to the density of 2 x 105 cells/well, culturing overnight for 14 +/-2 h at 37 ℃ in a 5% CO2 incubator, discarding the culture solution, adding 190 mu L of DMEM culture solution (without serum), 5 mu L of LPS working solution and 5 mu L of sample to be detected (or PBS buffer solution) into each well, incubating and culturing for 24h at 37 ℃ and 5% CO2, sucking the supernatant, and determining the NO content according to the instruction of a nitric oxide detection kit.
As a result: FIGS. 3 to 4
3. Relieving efficacy
The method comprises the following steps: HEK293 cells are inoculated in a 48-well plate according to the density of 5 multiplied by 104 cells/well, cultured overnight for 14 +/-2 h at 37 ℃ in a 5% CO2 incubator, the culture solution is discarded, 180 mu L of physiological saline (containing 2mM CaCl2) +10 mu L of sample to be detected (or PBS buffer solution) is added into each well, incubation culture is carried out for 1h, 10 mu L of capsaicin solution is added, incubation culture is carried out for 30min, the supernatant is discarded, the cells are washed for 3 times by the PBS buffer solution, the operation is carried out according to the instruction of the tissue calcium ion concentration fluorescence quantitative detection kit, and the fluorescence intensity is measured in a chemiluminescence gel imaging system.
As a result: FIG. 5
The invention screens anoectochilus formosanus extract (mainly containing anoectochilus formosanus glycoside) capable of inhibiting NO release from anoectochilus formosanus by adopting a RAW264.7 cell model, and designs and optimizes the preparation method of the extract. Meanwhile, the extract can inhibit the activation of TRPV1 on human embryonic kidney cells caused by capsaicin stimulation, reduce the calcium ion influx of cells caused by inflammatory mediators, avoid causing corresponding physiological changes (such as pain) and the like, and further prove that the extract has the effect of relieving.
While the foregoing description shows and describes the preferred embodiments of the present invention, it is to be understood that the invention is not limited to the forms disclosed herein, but is not to be construed as excluding other embodiments and is capable of use in various other combinations, modifications, and environments and is capable of changes within the scope of the inventive concept as described herein, commensurate with the above teachings, or the skill or knowledge of the relevant art. And that modifications and variations may be effected by those skilled in the art without departing from the spirit and scope of the invention as defined by the appended claims.
Reference documents:
[1]effect of Anoectochilus roxburghii extract on xanthine oxidase activity and hyperuricemia mice]Strait pharmaceuticals, 2017,29(07):12-14.
[1]Study of ethanol extracts of Zhenzzhi Yun, Chenqianwei, Jiantao, Chenwanqiu, Houjie, Tangchunling and Anoectochilus formosanus on anti-aging effect of naturally aging mice [ J]The Chinese medicinal materials 2020,43(05):1200-1204.
[1]Research progress of liver protection and action mechanism of Anoectochilus roxburghii (J)]Modern Chinese traditional medicine 1-13.
[1]Chen Y,Lyga J.Brain-Skin Connection:Stress,Inflammation and Skin Aging[J].Inflammation&Allergy- Drug Targets(Formerly Current Drug Targets-Inflammation&Allergy),2014,13(3).
[1]Inhibition of TRPV1 for the treatment of sensitive skin[J].Experimental Dermatology,2010,19(11):980-986.
[1]Du X M,Sun N Y,Tamura T,et al.Higher yielding isolation of kinsenoside in Anoectochilus and its antihyperliposis effect.[J].Biological&Pharmaceutical Bulletin,2001,24(1):65-69。
Claims (7)
1. A separation and purification method of anoectochilus formosanus glycoside is characterized in that: comprises the following process steps:
(1) pulping: putting fresh anoectochilus formosanus and water into a pulping machine according to a proportion and pulping into slurry;
(2) coarse filtration: conveying the feed liquid to a bag filter for filtering, and collecting filtrate; washing the filter residue with water, and collecting the filtrate; mixing the two filtrates;
(3) fine filtering: fine filtering the filtrate with diatomite filter, and collecting fine filtrate;
(4) purifying: after the fine filtrate is pressurized by a pump, impurities in the feed liquid are removed through an activated carbon column and an ion exchange resin column in sequence, and the permeate liquid is collected;
(5) concentration: concentrating the permeate at low temperature in vacuum to obtain extract;
(6) and (3) extraction: mixing the extract with an extractant, standing, and collecting supernatant;
(7) concentrating and drying: concentrating the supernatant under reduced pressure to obtain the anoectochilus formosanus glycoside.
2. The method for separating and purifying acteoside as claimed in claim 1, wherein the method comprises the steps of: the ion exchange resin used is a cation exchange resin.
3. The method for separating and purifying acteoside as claimed in claim 1, wherein the method comprises the steps of: the extractant is one or a mixture of ethyl acetate and absolute ethyl alcohol.
4. The method for separating and purifying acteoside as claimed in claim 1, wherein the method comprises the steps of: the ion exchange resin used was LX-S1860 ion exchange resin.
5. The method for separating and purifying acteoside as claimed in claim 1, wherein the method comprises the following steps: the extractant is a mixture of ethyl acetate and absolute ethyl alcohol, and the ratio of the absolute ethyl alcohol to the ethyl acetate is 2: 8.
6. Use of the roxburgh anoectochilus terminal bud glycoside in a soothing cosmetic, characterized in that the roxburgh anoectochilus terminal bud glycoside extracted according to claim 1, 2, 3, 4 or 5 is added to a soothing cosmetic in an amount of 0.01% to 20%.
7. The use of an anoectochilus formosanus glycoside in a soothing cosmetic as claimed in claim 6, wherein: the roxburgh anoectochilus terminal bud glycoside is added into the cosmetics with the relieving effect, and the addition amount is 2% -5%.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202111561937.2A CN114634535B (en) | 2021-12-20 | 2021-12-20 | Separation and purification method of anoectochilus formosanus glycoside and application of anoectochilus formosanus glycoside in soothing cosmetics |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202111561937.2A CN114634535B (en) | 2021-12-20 | 2021-12-20 | Separation and purification method of anoectochilus formosanus glycoside and application of anoectochilus formosanus glycoside in soothing cosmetics |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN114634535A true CN114634535A (en) | 2022-06-17 |
| CN114634535B CN114634535B (en) | 2024-08-23 |
Family
ID=81946443
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202111561937.2A Active CN114634535B (en) | 2021-12-20 | 2021-12-20 | Separation and purification method of anoectochilus formosanus glycoside and application of anoectochilus formosanus glycoside in soothing cosmetics |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN114634535B (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN116898780A (en) * | 2023-08-31 | 2023-10-20 | 广州远想生物科技股份有限公司 | Anoectochilus roxburghii extract and preparation process and application thereof |
Citations (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5994533A (en) * | 1997-08-11 | 1999-11-30 | Council Of Scientific & Industrial Research | Process for the recovery of tartaric acid and other products from tamarind pulp |
| US20090306199A1 (en) * | 2008-03-19 | 2009-12-10 | Wen-Chuan Lin | Method for inhibiting activation of macrophages, inhibiting formation of osteoclasts, inhibiting function of osteoclasts, and/or activating osteoblasts |
| KR20100102439A (en) * | 2009-03-11 | 2010-09-24 | 주식회사 한국화장품제조 | A cosmetic composition comprising an extract of tissue cultured anoectochilus formosanus and a preparation method of the extract of tissue cultured anoectochilus formosanus |
| CN105037462A (en) * | 2015-08-19 | 2015-11-11 | 福建金草生物集团股份有限公司 | Extraction method of kinsenoside |
| CN109394777A (en) * | 2018-12-12 | 2019-03-01 | 上海交通大学医学院附属第九人民医院 | Application of the anoectochilus roxburghii glycosides in osteoarthritis treatment drug |
| CN112587618A (en) * | 2020-12-30 | 2021-04-02 | 联和东浩(厦门)药业有限公司 | Method for separating anoectochilus formosanus total alkaloids from anoectochilus formosanus extract |
| CN112870132A (en) * | 2021-04-07 | 2021-06-01 | 福建宸润生物科技有限公司 | Freckle-removing and whitening mask liquid and mask containing same |
| CN112898357A (en) * | 2021-01-29 | 2021-06-04 | 上海中医药大学 | Diterpene glycoside novel compound in trollius chinensis bunge and separation and purification method and application thereof |
-
2021
- 2021-12-20 CN CN202111561937.2A patent/CN114634535B/en active Active
Patent Citations (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5994533A (en) * | 1997-08-11 | 1999-11-30 | Council Of Scientific & Industrial Research | Process for the recovery of tartaric acid and other products from tamarind pulp |
| US20090306199A1 (en) * | 2008-03-19 | 2009-12-10 | Wen-Chuan Lin | Method for inhibiting activation of macrophages, inhibiting formation of osteoclasts, inhibiting function of osteoclasts, and/or activating osteoblasts |
| KR20100102439A (en) * | 2009-03-11 | 2010-09-24 | 주식회사 한국화장품제조 | A cosmetic composition comprising an extract of tissue cultured anoectochilus formosanus and a preparation method of the extract of tissue cultured anoectochilus formosanus |
| CN105037462A (en) * | 2015-08-19 | 2015-11-11 | 福建金草生物集团股份有限公司 | Extraction method of kinsenoside |
| CN109394777A (en) * | 2018-12-12 | 2019-03-01 | 上海交通大学医学院附属第九人民医院 | Application of the anoectochilus roxburghii glycosides in osteoarthritis treatment drug |
| CN112587618A (en) * | 2020-12-30 | 2021-04-02 | 联和东浩(厦门)药业有限公司 | Method for separating anoectochilus formosanus total alkaloids from anoectochilus formosanus extract |
| CN112898357A (en) * | 2021-01-29 | 2021-06-04 | 上海中医药大学 | Diterpene glycoside novel compound in trollius chinensis bunge and separation and purification method and application thereof |
| CN112870132A (en) * | 2021-04-07 | 2021-06-01 | 福建宸润生物科技有限公司 | Freckle-removing and whitening mask liquid and mask containing same |
Non-Patent Citations (1)
| Title |
|---|
| 张锦文等: ""高效液相色谱法测定金线莲中金线莲苷的含量"", 《中国医院药学杂志》, vol. 31, no. 4, pages 261 - 263 * |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN116898780A (en) * | 2023-08-31 | 2023-10-20 | 广州远想生物科技股份有限公司 | Anoectochilus roxburghii extract and preparation process and application thereof |
| CN116898780B (en) * | 2023-08-31 | 2024-12-10 | 广州远想生物科技股份有限公司 | Aromatic thread vine extract and its preparation process and application |
Also Published As
| Publication number | Publication date |
|---|---|
| CN114634535B (en) | 2024-08-23 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN101463061B (en) | Ginseng saponin Rg1 and Rb1 in pseudo-ginseng and preparation of total saponin thereof | |
| CN102526315B (en) | Preparation method of extracts of effective fractions of lychee seeds | |
| CN102816066A (en) | Method for extracting chlorogenic acid and hyperoside from lonicera japonica leaves | |
| CN101508711B (en) | Method for separating and purifying flavonoid glycoside monomers from Mimosa pudica | |
| CN101229335A (en) | Method for Enzymatically Preparing Smilax Smilax Total Saponins Extract | |
| CN106977559A (en) | A kind of method of separating-purifying punicalagins and gallic acid simultaneously from granatum | |
| CN114634535B (en) | Separation and purification method of anoectochilus formosanus glycoside and application of anoectochilus formosanus glycoside in soothing cosmetics | |
| CN101623309A (en) | Technology for extracting Chinese actinidia root antitumor active site | |
| CN101830881A (en) | Enzyme-induced method for efficiently extracting toxifolin from larch processing leftover | |
| CN105294793B (en) | The separation method of aurantiin in aizoon stonecrop | |
| CN105030914B (en) | Application of the Ligustrum robust Folum Ilicis extract in alpha-glucosidase restrainer | |
| CN110467643A (en) | Cerebronic method and cerebronic purposes are extracted from Gynura procumbens (Lour.) Merr | |
| CN107188912A (en) | A kind of NHDC obtained from Oxytropis Species | |
| CN103739660B (en) | A kind of compound, its extracting method, its application preparing antitumor drug and antitumor drug of preparation thereof | |
| CN109180471A (en) | Water cape jasmine monoterpenes compound crocusatinN and jasminosideB preparation method and application | |
| CN114146124A (en) | Application of Polymethoxyflavonoids of Citrus Citrus in the Preparation of Antioxidants | |
| CN104059123B (en) | The antitumor drug of a kind of Sasanguasaponin compound, its preparation method, application and preparation thereof | |
| CN103833818B (en) | The antitumor drug of a kind of Sasanguasaponin compound, its preparation method, application and preparation thereof | |
| CN102631386B (en) | Bupleurum antipyretic and analgesic preparation and technology for preparing same | |
| CN103113196B (en) | Glechoma longituba phenol, and preparation method and application thereof | |
| KR101416674B1 (en) | Ginseng prosapogenin high concentration containing American ginseng preparation using sonication and process for thereof | |
| CN113425647A (en) | Preparation method and application of blumea balsamifera dreg total flavone extract | |
| CN112794923A (en) | Ligusticum chuanxiong polysaccharide and its preparation method, identification method and application | |
| CN105106357A (en) | Method for preparing extract with alpha-glucosidase restraining effect | |
| CN105030837B (en) | A kind of method of extracting and developing hippocampus trimaculatus Leacs liposoluble constituent |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant |