CN114602324B - A clip-on membrane replacement system for environmental DNA sampling - Google Patents

Abstract

The invention provides a clamping filter membrane replacement system that can be used for environmental DNA sampling. The filter membrane is assembled into a filter cake with a ring-shaped shell. The filter membrane replacement system includes: a magazine-type mechanism, a magazine-type mechanism There are several filter cakes to be sampled inside; the sampling area is used to receive the filter cakes to be sampled, and to collect water samples from the filter cakes to be sampled; the clamping device includes a clamp, and the clamp is used to The filter cake to be sampled is clamped to the sampling area for water sample collection, which avoids errors caused by manual replacement of the filter membrane due to different techniques, and the method of clamping the filter cake through the clamp greatly improves the accuracy of filter membrane replacement.

Description

A clip-on membrane replacement system for environmental DNA sampling

technical field

The invention belongs to the technical field of water sample collection, and in particular relates to a clamping filter membrane replacement system which can be used for environmental DNA sampling.

Background technique

With the advancement of the new global biodiversity governance strategy and the implementation of the United Nations Convention on Biological Diversity, the importance of biodiversity research has become increasingly prominent. With the development of biodiversity investigation and research, large-scale and high-frequency metagenomic analysis of environmental DNA and microorganisms in water bodies is usually required, which requires large-scale filtration of water samples and frequent replacement of filters. membrane. Different from ordinary filter replacement scenarios, in environmental DNA sampling or microbial sampling scenarios, special attention needs to be paid to avoid cross-contamination between samples and avoid introducing new pollution during the operation process, so as to ensure the independence of samples and the accuracy of test results sex. In the past, the simple and direct method of replacing the filter membrane was that after the water sample was filtered through the disc filter, the experimenter manually opened the filter head and took out the filter membrane containing the sample with clean tweezers, and then used the clean tweezers to pick up a new disc filter membrane Replace it, and finally reinstall the filter head manually. Whether it is applied to long-term continuous monitoring at a fixed location, or to follow the survey ship to sample multiple locations sequentially, it means large-scale and high-frequency replacement of the disc filter operation. The main problems and deficiencies in the prior art include: due to the complicated operation of the current method of manually replacing the disc filter membrane, different experimenters may introduce errors due to different operating methods. Moreover, this dismantling-film removal-replacement-reinstallation operation process consumes a lot of time and labor in vain, which is not conducive to high-frequency sampling. Thirdly, manual replacement of the filter membrane is also easy to introduce contamination, resulting in deviation of the sample survey results. Finally, manual replacement of the filter membrane is inseparable from human operation, which is not conducive to automatic sampling and filtration, nor is it conducive to standardized long-term continuous sampling or online continuous monitoring activities. Pneumatic adsorption replacement membrane solutions used in other fields easily lead to cross-contamination between membranes. The rotary disc membrane replacement scheme used in other fields can accommodate a small number of membranes, which cannot meet the application of large-scale and high-frequency disk membrane replacement scenarios.

That is to say, in terms of the replacement method of the filter membrane, in the prior art, manual replacement of the filter membrane has the disadvantages of complicated operation, different experimenters may introduce errors due to different operating methods, and the replacement efficiency is low.

It can be seen that for the replacement method of the filter membrane, how to increase the replacement speed, reduce the error during the replacement process, and then improve the replacement efficiency of the filter membrane is a technical problem urgently needed to be solved by those skilled in the art.

Contents of the invention

The present invention provides a clip-on membrane replacement system that can be used for environmental DNA sampling to at least solve the above technical problems:

In order to solve the above problems, the first aspect of the present invention provides a clip-on membrane replacement system that can be used for environmental DNA sampling, the filter membrane is assembled into a filter cake with an annular shell, the filter membrane The replacement system includes: a magazine-type mechanism, the inside of which is provided with a number of filter cakes to be sampled; a sampling area, which is used to receive the filter cakes to be sampled, and The filter cake is subjected to water sample collection; a clamping device, the clamping device includes a clamp, and the clamp is used to clamp the filter cake to be sampled to the sampling area.

In the first aspect, the sampling area includes a semicircular base, a top cover is provided on one side of the upper part of the base, and a cake holding space for receiving the filter cake is formed between the base and the top cover; The magazine-type mechanism includes a magazine barrel, in which several filter cakes to be sampled are stacked up and down, the magazine barrel is located on the other side of the upper part of the base, and the magazine barrel The upper part of the cartridge away from the base leaves a cake passage that can pass through a filter cake height; the clamping device also includes a first driving device, which is used to perform a rotating action, and the first driving device The device is set at the center of the base, and is connected with the clamp for driving the clamp to rotate around the center of the circle; wherein, the cake-holding space and the cake-passing channel are located at the center of the clamp around the When the first driving device executes the rotation action and makes the clamp reciprocate along the cake passing channel to the cake holding space, all the samples to be sampled in the channel will be moved one by one. The filter cake is clamped to the cake holding space for water sample collection.

In the first aspect, the clamp includes: a left claw finger, a right claw finger and a second driving device, the left claw finger and the right claw finger are arc-shaped, the left claw finger and the right claw finger One end of the finger is coupled with the second driving device through a gear set. When the second driving device is driven, the gear set drives the ends of the left claw finger and the right claw finger away from the coupling end to open and close together. The left claw finger and the right claw finger jointly form a clamping space adapted to the outer edge of the filter cake.

In the first aspect, the opposite sides of the left claw and the right claw are provided with anti-slip layers.

In the first aspect, the membrane replacement system includes: a detection device, the detection device is used to detect the position of the clamp and generate position information; a control device, the control device is connected to the detection device, and to obtain the position information; the control device is also connected to the first drive device and the second drive device; wherein, when the position information received by the control device is a preset position, the control device The first driving device executes the rotation action, and controls the second driving device to drive.

In the first aspect, the detection device includes a camera.

In the first aspect, the detection device includes an angle sensor, the angle sensor is arranged at the driving end of the first driving device, and when the first driving device performs a rotation action, it detects the angle of the first driving device. Whether the rotation angle reaches the preset rotation angle, if yes, it is determined that the first driving device rotates to the preset position.

In the first aspect, the membrane replacement system includes: a third driving device, the driving end of the third driving device is connected to the clamp, and the third driving device is used to perform a tangential rotation action, when the When the third driving device executes the tangential action, the clamp is rotated along the horizontal tangential direction.

In the first aspect, the first driving device, the second driving device and the third driving device each include one of the following motors: a stepping motor or a servo motor.

Beneficial effects: the present invention proposes a clip-type filter membrane replacement system that can be used for environmental DNA sampling, by setting a number of filter cakes in the magazine-type mechanism, and then performing the filter cake in the magazine-type mechanism through the clamping device Clamping, to be transported to the sampling area for water sample collection, the clamping device includes a clamp, and the mechanization of filter membrane replacement is realized through the clamping method, which solves the problem of manual filter membrane replacement in the prior art due to different methods. resulting in errors.

Description of drawings

In order to more clearly illustrate the embodiments of this specification or the technical solutions in the prior art, the following will briefly introduce the accompanying drawings required in the embodiments. Obviously, the accompanying drawings in the following description are only some of the present invention. Embodiments, for those of ordinary skill in the art, other drawings can also be obtained based on these drawings without any creative effort.

FIG. 1 is a structural diagram 1 of a clip-on membrane replacement system that can be used for environmental DNA sampling in Example 1 of the present invention;

Fig. 2 is a structural diagram 2 of a clip-on membrane replacement system that can be used for environmental DNA sampling in Example 1 of the present invention;

Fig. 3 is a top view of a clip-on membrane replacement system that can be used for environmental DNA sampling in Example 1 of the present invention;

Fig. 4 is a schematic diagram of the position change of the filter cake clamped by the clamp.

Explanation of reference signs:

1. Magazine mechanism;

101. Magazine barrel;

2. Base;

3. Fixtures;

301. Left claw finger;

302. Right claw finger;

4. Filter cake;

5. The first driving device;

6. The second driving device;

7. The fourth driving device;

8. Limit rod;

9. Top cover;

10. Spring;

11. Limiting piece;

12. Limit column;

13. Crank lever;

14. Water inlet pipe;

15. Outlet pipe;

16. Magazine through hole;

17. Shaft;

18. Fixed frame;

19, the fixing frame through hole.

Detailed ways

The technical solutions in the present invention will be clearly and completely described below in conjunction with the accompanying drawings. Apparently, the described embodiments are only some, not all, embodiments of the present invention. All other embodiments obtained by persons of ordinary skill in the art based on the embodiments of the present invention belong to the protection scope of the present invention.

Meanwhile, in the embodiments of the present specification, when a component is referred to as being “fixed” to another component, it may be directly on the other component or there may be an intervening component. When a component is said to be "connected" to another component, it may be directly connected to the other component or there may be intervening components at the same time. When a component is said to be "set on" another component, it may be set directly on the other component or there may be an intervening component at the same time. The terms "vertical", "horizontal", "left", "right" and similar expressions used in the embodiments of this specification are for the purpose of illustration only, and are not intended to limit the present invention.

Embodiment one:

As shown in Figures 1-4, the first embodiment provides a clip-on filter membrane replacement system that can be used for environmental DNA sampling. The filter membrane is assembled into a filter cake 4 with a ring-shaped shell, which is characterized in that , The filter membrane replacement system includes: a magazine mechanism 1, a sampling area, and a clamping device;

The interior of the magazine mechanism 1 is provided with a number of filter cakes 4 to be sampled;

The sampling area is used to receive the filter cake 4 to be sampled, and collect water samples from the filter cake 4 to be sampled;

The clamping device includes a clamp 3, which is used to clamp the filter cake 4 to be sampled to the sampling area for water sample collection.

In the technical solution of the first embodiment above, a number of filter cakes 4 are set in the magazine-type mechanism 1, and then the filter cakes 4 in the magazine-type mechanism 1 are clamped by the clamping device to be transported to the sampling area For water sample collection, the clamping device includes a clamp 3, which realizes the mechanization of filter membrane replacement through the clamping method of the clamp 3, and solves the error caused by manual filter membrane replacement in the prior art due to different techniques. The magazine mechanism 1 includes a magazine barrel 101 , and the magazine barrel 101 is fixed by a fixing frame 18 , and the side wall of the fixing frame 18 is provided with a fixing frame through hole 19 .

Further, for the membrane replacement system in the above-mentioned first embodiment, this first embodiment proposes an implementation method, which includes: the sampling area includes a semicircular base 2, and a top cover is provided on the upper side of the base 2 9. A cake holding space for receiving filter cake 4 is formed between the base 2 and the top cover 9; the magazine mechanism 1 includes a magazine barrel 101, and several filter cakes 4 to be sampled in the magazine barrel 101 are stacked up and down Set, the magazine barrel 101 is located on the other side of the base 2, and the magazine barrel 101 is reserved from the upper surface of the base 2 to pass through a cake passage at a height of a filter cake 4; the clamping device also includes a first driving device 5 , the first driving device 5 is used to perform the rotation action, the first driving device 5 is arranged at the center of the semicircular base 2, and is connected with the clamp 3, and is used to drive the clamp 3 to rotate around the center of the circle; wherein, the cake holding space and The cake passages are all located on the path where the clamp 3 rotates around the center of the circle; when the first driving device 5 executes the rotation action and makes the clamp 3 reciprocate along the cake passage to the cake holding space, the cakes to be sampled in the cake passage will be sampled one by one. The filter cake 4 is clamped to the cake holding space for water sample collection.

Specifically, for the clamp 3, the first embodiment proposes an implementation, which includes: the left claw 301, the right claw 302 and the second driving device 6, the left claw 301 and the right claw 302 All are arc-shaped, and one end of the left claw finger 301 and the right claw finger 302 is coupled with the second drive device 6 through a gear set. When the second drive device 6 is driven, the gear set drives the left claw finger 301 and the right claw finger 302 away from the coupling end. One end is opened and closed together, and the left claw finger 301 and the right claw finger 302 together form a cake holding space suitable for the outer edge of the filter cake 4; and the opening angle of the left claw finger 301 and the right claw finger 302 is greater than 90 degrees;

Further, based on the basis that the filter cake 4 is clamped by the clamp 3 in the first embodiment above, the first embodiment proposes a technical solution for detecting components to improve the accuracy of clamping. The technical solution includes: a detection device, The detection device is used to detect the position of the clamp 3 and generate position information; the control device is connected to the detection device for obtaining position information; the control device is also connected to the first drive device 5 and the second drive device 6; wherein, When the position information received by the control device is the preset position, the first driving device 5 is controlled to perform a rotation action, and the second driving device 6 is controlled to drive.

Furthermore, for the detection device, the first embodiment proposes an implementation manner, which includes: a camera, through which the position information of the fixture 3 is obtained.

Further, for the detection device, the first embodiment proposes another implementation, which includes: an angle sensor, the angle sensor is arranged at the driving end of the first driving device 5, when the first driving device 5 performs a rotation action , detect whether the rotation angle of the first driving device 5 reaches the preset rotation angle, if so, determine that the first driving device 5 rotates to the preset position;

Furthermore, for the detection device, the first embodiment also proposes an implementation, which includes combining a camera and an angle sensor to jointly detect the position accuracy of the fixture 3 .

For the filter membrane replacement system in the above-mentioned first embodiment, this first embodiment also proposes an implementation, which includes: a third driving device, the driving end of the third driving device is connected to the clamp 3, and the third driving The device is used to perform a tangential rotation, and when the third driving device performs a tangential movement, the gripper 3 is rotated in a horizontal tangential direction to turn over the filter membrane located in the filter cake 4 .

Specifically, for the first driving device 5, the second driving device 6 and the third driving device, as the first driving device 5, the second driving device 6 and the third driving device is an embodiment, this embodiment Example 1 proposes a solution, which includes: the first drive device 5, the second drive device 6 and the third drive device can be stepper motors or servo motors.

Further, this embodiment 1 also proposes an implementation, which includes: the filter membrane replacement system also includes: a spring 10, a limit piece 11 and a limit rod 8, and the limit rod 8 is passed through the top cover 9 to The base 2 and the top cover 9 can slide up and down along the limit rod 8, the spring 10 is sleeved on the limit rod 8, the limit piece 11 is arranged on the end of the limit rod 8 away from the base 2, and the spring 10 is limited to the limit Between the sheet 11 and the top cover 9, when the spring 10 is in the state of automatic extension, the top cover 9 is pressed on the base 2; the limit post 12, the stop post 12 is vertically arranged, and the top of the stop post 12 is a cone shape; a pair of crank lever 13, the crank lever 13 is made of elastic metal material, the crank lever 13 is a long rod structure, the two ends of the long rod structure are protrudingly provided with interference parts, and the middle part of the long rod structure is connected with the magazine type mechanism through the rotating shaft 17 1, the interference part at one end of the long rod structure is in contact with the through hole 16 of the magazine on the corresponding side and is in contact with the filter cake 4, and the interference part at the other end of the long rod structure is in contact with the upper part of the limit post 12; When the cover 9 is in the initial position of the initial rise, a pair of interference parts of the long rod structure corresponding to the filter cake 4 and the limit post 12 naturally contact, when the top cover 9 is displaced upward, the conical tapered surface of the limit post 12 pushes the long The interference part at the other end of the rod structure, the long rod structure rotates around the rotating shaft 17, so that the interference part at the other end of the long rod structure is in close contact with the filter cake 4 on the corresponding side; the connecting plate, one end of the connecting plate is fixedly connected with the top cover 9, connected The upper surface of the plate is used to carry the limit column 12; the fourth driving device 7, the driving end of the fourth driving device 7 is arranged on the lower surface of the connecting plate, and the fourth driving device 7 is used to perform the stretching action and the shrinking action; It should be noted that, when the filter membrane is clamped by the clamp 3, the fourth driving device 7 pushes the connecting plate to move upward, so that the connecting plate pushes the limit post 12, so that the limit post 12 applies force to the crank lever 13, so that The crank lever 13 clamps the filter cake 4 in the through hole 16 of the magazine, so that the filter cake 4 in the cake passing channel can be clamped smoothly. When the clamp 3 is separated from the cake passing channel, the fourth driving mechanism drives the connecting plate to reset, In order to make the filter cake 4 in the magazine barrel 101 smoothly fall into the cake passage. Wherein, the top cover is provided with a water inlet pipe 14 for introducing an external water source to be sampled, and the base is provided with a water outlet pipe 15 for discharging the collected water.

Finally, it should be noted that: the above-mentioned embodiments are only specific implementations of the present invention, to illustrate the technical solutions of the present invention, rather than to limit them, and the scope of protection of the present invention is not limited thereto. This example has described the present invention in detail, and those skilled in the art should understand that any person familiar with the technical field can still modify or modify the technical solutions described in the foregoing embodiments within the technical scope disclosed in the present invention. Changes can be easily thought of, or equivalent replacements can be made to some of the technical features; and these modifications, changes or replacements do not make the essence of the corresponding technical solutions depart from the scope of the technical solutions of the embodiments of the present invention. All should be covered within the protection scope of the present invention. Therefore, the protection scope of the present invention should be based on the protection scope of the claims.

Although the embodiment of the present invention has been disclosed as above, it is not limited to the use listed in the specification and implementation, it can be applied to various fields suitable for the present invention, and it can be easily understood by those skilled in the art Therefore, the invention is not limited to the specific details and examples shown and described herein without departing from the general concept defined by the claims and their equivalents.

Claims (8)

1. A clamping filter membrane replacement system for environmental DNA sampling, the filter membrane being assembled as a filter cake of unitary construction by an annular housing, the filter membrane replacement system comprising:

the cartridge magazine type mechanism is internally provided with a plurality of filter cakes to be sampled;

the sampling area is used for receiving the filter cake to be sampled and collecting a water sample of the filter cake to be sampled; the sampling area comprises a semicircular base, a top cover is arranged on one side of the upper part of the base, and a cake containing space for receiving the filter cake is formed between the base and the top cover;

the magazine type mechanism comprises a magazine barrel, a plurality of filter cakes to be sampled in the magazine barrel are arranged in a vertically stacked mode, the magazine barrel is positioned on the other side of the upper part of the base, and a cake passing channel capable of passing through the height of one filter cake is reserved between the magazine barrel and the upper part of the base;

the clamping device comprises a clamp which is used for clamping a filter cake to be sampled to the sampling area;

the clamping device further comprises a first driving device, wherein the first driving device is used for executing a rotating action, is arranged at the circle center of the base, is in driving connection with the clamp and is used for driving the clamp to rotate around the circle center;

wherein, the cake containing space and the cake passing channel are both positioned on a path of the clamp rotating around the circle center; when the first driving device executes a rotating action and enables the clamp to do reciprocating rotation along the cake passing channel to the cake containing space, the filter cakes to be sampled in the cake passing channel are clamped to the cake containing space one by one so as to collect water samples;

the filter membrane replacement system further comprises: the limiting column is vertically arranged, and the upper part of the limiting column is conical; the crank lever is made of elastic metal, the crank lever is of a long rod structure, two ends of the long rod structure are provided with interference parts in a protruding mode, the middle of the long rod structure is connected with the outer wall of the magazine type mechanism through a rotating shaft, one end interference part of the long rod structure is arranged in a magazine through hole on the corresponding side and is in contact with a filter cake, and the other end interference part of the long rod structure is in contact with the upper part of the limit post; when the top cover is in an initial position of initial rising, a pair of interference parts of the long rod structure naturally contact with the corresponding filter cakes and the limiting columns, and when the top cover is displaced upwards, the conical surface of the limiting columns pushes the interference parts of the other ends of the long rod structure, and the long rod structure rotates around the rotating shaft, so that the interference parts of the other ends of the long rod structure are in close contact with the filter cakes on the corresponding sides; one end of the connecting plate is fixedly connected with the top cover, and the upper surface of the connecting plate is used for bearing the limit column; and the driving end of the fourth driving device is arranged on the lower surface of the connecting plate, and the fourth driving device is used for executing the extending action and the contracting action.

2. The system for changing a filter membrane for environmental DNA sampling of claim 1, wherein said clamp comprises:

the device comprises a left claw finger, a right claw finger and a second driving device, wherein the left claw finger and the right claw finger are arc-shaped, one ends of the left claw finger and the right claw finger are coupled with the second driving device through a gear set, when the second driving device is driven, the gear set drives the left claw finger and the right claw finger to open and close together at one end far away from the coupling end, and the left claw finger and the right claw finger jointly form a clamping space which is suitable for the outer edge of a filter cake.

3. The system for changing a filter membrane for environmental DNA sampling of claim 2, wherein:

the left claw finger and the right claw finger are both provided with anti-slip layers on opposite sides.

4. The system of claim 2, wherein the filter exchange system comprises:

the detection device is used for detecting the position of the clamp and generating position information;

the control device is connected with the detection device and used for acquiring the position information; the control device is also connected with the first driving device and the second driving device;

when the position information received by the control device is a preset position, the first driving device is controlled to execute the rotation action, and the second driving device is controlled to be driven.

5. The system for changing a filter membrane for environmental DNA sampling of claim 4, wherein:

the detection device comprises a camera.

6. The system for changing a filter membrane for environmental DNA sampling of claim 4, wherein:

the detection device comprises an angle sensor, the angle sensor is arranged at the driving end of the first driving device, when the first driving device executes a rotation action, whether the rotation angle of the first driving device reaches a preset rotation angle is detected, and if yes, the first driving device is judged to rotate to a preset position.

7. The system of claim 2, wherein the filter exchange system comprises:

and the driving end of the third driving device is connected with the clamp, and the third driving device is used for executing tangential rotation, so that the clamp rotates along the horizontal tangential direction when the third driving device executes the tangential rotation.

8. The system of claim 7, wherein the first drive, the second drive, and the third drive each comprise one of the following motors:

a stepper motor or a servo motor.

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