CN114594208A - Real-time detection device for virus spread to epidemic disease in air environment - Google Patents
Real-time detection device for virus spread to epidemic disease in air environment Download PDFInfo
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- CN114594208A CN114594208A CN202210201049.8A CN202210201049A CN114594208A CN 114594208 A CN114594208 A CN 114594208A CN 202210201049 A CN202210201049 A CN 202210201049A CN 114594208 A CN114594208 A CN 114594208A
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- 241000700605 Viruses Species 0.000 title claims abstract description 48
- 238000011897 real-time detection Methods 0.000 title claims abstract description 7
- 201000010099 disease Diseases 0.000 title abstract description 9
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 title abstract description 9
- 239000003570 air Substances 0.000 claims abstract description 56
- 239000000427 antigen Substances 0.000 claims abstract description 36
- 102000036639 antigens Human genes 0.000 claims abstract description 36
- 108091007433 antigens Proteins 0.000 claims abstract description 36
- 230000008859 change Effects 0.000 claims abstract description 13
- 239000012080 ambient air Substances 0.000 claims abstract description 3
- 238000001514 detection method Methods 0.000 abstract description 21
- 238000004891 communication Methods 0.000 abstract description 10
- 230000002093 peripheral effect Effects 0.000 abstract description 6
- 230000000644 propagated effect Effects 0.000 abstract 1
- 239000000523 sample Substances 0.000 description 44
- 239000007788 liquid Substances 0.000 description 9
- 230000003612 virological effect Effects 0.000 description 8
- 230000009471 action Effects 0.000 description 7
- 238000000034 method Methods 0.000 description 7
- 150000007523 nucleic acids Chemical class 0.000 description 7
- 102000039446 nucleic acids Human genes 0.000 description 7
- 108020004707 nucleic acids Proteins 0.000 description 7
- 238000005336 cracking Methods 0.000 description 5
- 239000011159 matrix material Substances 0.000 description 4
- 238000007789 sealing Methods 0.000 description 4
- 230000009089 cytolysis Effects 0.000 description 3
- 230000008105 immune reaction Effects 0.000 description 3
- 239000007791 liquid phase Substances 0.000 description 3
- VYPSYNLAJGMNEJ-UHFFFAOYSA-N Silicium dioxide Chemical compound O=[Si]=O VYPSYNLAJGMNEJ-UHFFFAOYSA-N 0.000 description 2
- 208000015181 infectious disease Diseases 0.000 description 2
- 239000006166 lysate Substances 0.000 description 2
- 239000000463 material Substances 0.000 description 2
- 238000005070 sampling Methods 0.000 description 2
- 238000012216 screening Methods 0.000 description 2
- 239000000741 silica gel Substances 0.000 description 2
- 229910002027 silica gel Inorganic materials 0.000 description 2
- 241000711573 Coronaviridae Species 0.000 description 1
- 102000004190 Enzymes Human genes 0.000 description 1
- 108090000790 Enzymes Proteins 0.000 description 1
- 206010061218 Inflammation Diseases 0.000 description 1
- 206010035664 Pneumonia Diseases 0.000 description 1
- 230000002159 abnormal effect Effects 0.000 description 1
- 239000012190 activator Substances 0.000 description 1
- 238000004140 cleaning Methods 0.000 description 1
- 230000034994 death Effects 0.000 description 1
- 230000007812 deficiency Effects 0.000 description 1
- 238000010586 diagram Methods 0.000 description 1
- 239000002270 dispersing agent Substances 0.000 description 1
- 238000001962 electrophoresis Methods 0.000 description 1
- 230000036046 immunoreaction Effects 0.000 description 1
- 230000004054 inflammatory process Effects 0.000 description 1
- 239000012071 phase Substances 0.000 description 1
- 230000002265 prevention Effects 0.000 description 1
- 230000008569 process Effects 0.000 description 1
- 230000009467 reduction Effects 0.000 description 1
- 239000012488 sample solution Substances 0.000 description 1
- 239000000758 substrate Substances 0.000 description 1
- 239000013589 supplement Substances 0.000 description 1
- 230000001960 triggered effect Effects 0.000 description 1
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/0004—Gaseous mixtures, e.g. polluted air
- G01N33/0009—General constructional details of gas analysers, e.g. portable test equipment
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/569—Immunoassay; Biospecific binding assay; Materials therefor for microorganisms, e.g. protozoa, bacteria, viruses
- G01N33/56983—Viruses
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
- G01N35/00584—Control arrangements for automatic analysers
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- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Immunology (AREA)
- General Physics & Mathematics (AREA)
- Pathology (AREA)
- Physics & Mathematics (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Medicinal Chemistry (AREA)
- Hematology (AREA)
- Urology & Nephrology (AREA)
- Virology (AREA)
- Food Science & Technology (AREA)
- Biomedical Technology (AREA)
- Molecular Biology (AREA)
- Tropical Medicine & Parasitology (AREA)
- Cell Biology (AREA)
- Microbiology (AREA)
- Biotechnology (AREA)
- Combustion & Propulsion (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Abstract
The invention provides a real-time detection device for viruses propagated by epidemic diseases in an air environment, which comprises an ambient air sampler, a disposable air sample cracker, a disposable virus antigen reactor, a virus antigen reactor physical state change detector, an MCU (microprogrammed control Unit) and a peripheral circuit for device control, a communication circuit for communication, a video device for real-time portrait acquisition and the like. And when the virus detection value reaches a detection alarm threshold value, an alarm is started. The device can be respectively arranged in places where people flow or people are closed, so that the searching and controlling timeliness of epidemic virus carrying people is greatly improved.
Description
Technical Field
The invention relates to the field of epidemiological epidemic prevention detection and control caused by viruses, in particular to a real-time detection device for viruses spread to epidemic diseases in an air environment.
Background
The epidemic disease caused by virus is a common epidemic disease, particularly the epidemic pneumonia or other inflammations caused by the novel coronavirus which is troubling people in daily life at present cause considerable serious cases or death cases, and further cause panic or inconvenience in life of people. At present, the generally effective method adopted in China and the world is to carry out the examination and detection of all people aiming at the characteristic nucleic acid of the virus in a large scale, or carry out the nucleic acid detection and examine the possible contact personnel after the infected personnel have diseases, thereby effectively controlling the infection route at least in China. However, this method has a significant disadvantage of a delay in case detection. When nucleic acids are detected positive, the virus carrier may have been present in a multiple environment and cause other infections. In particular, if a person who does not perform nucleic acid screening as required is present, the nucleic acid screening method is not effective. As a supplement to the deficiency of the nucleic acid detection method, once the method is implemented, the method can be deployed and controlled in places with dense people flow or frequent exits, such as markets, airports, stations, offices, even cell entrances and the like, and can also be used as a large-scale nucleic acid detection pre-detection method.
The invention aims to fill the blank, thereby providing another effective method for human fighting against epidemic diseases caused by viruses.
Disclosure of Invention
The invention aims to provide a real-time detection device for viruses spread by epidemic diseases in an air environment.
The technical scheme adopted by the invention for solving the technical problem is as follows:
1. the invention relates to a real-time detection device for virus spread by convection in air environment, which is characterized by comprising an ambient air sampler, a disposable air sample cracker, a disposable virus antigen reactor, a virus antigen reactor physical state change detector, a micromotor for air sampling, an MCU (micro control unit) and a peripheral circuit for device control, a communication circuit for communication, a video device for real-time portrait acquisition and the like.
2. The air sampler as claimed in claim 1 comprises an air inlet in contact with air, an internal air channel for adjusting the intensity of air flow, a micro-motor and a fan for extracting an air sample, a one-way valve for controlling the internal pressure of the sampler, and a sample air storage container, wherein the sample air storage container is connected with the air sample cracker through the one-way valve. When the air storage container reaches the set pressure, the inlet one-way valve of the air storage container is closed, the outlet one-way valve is opened at the same time, and the air sample in the air storage container is injected into the air sample cracker.
3. The disposable air sample cracker of claim 1, in which one end of the air sampler is connected via a one-way valve inside the device and the other end of the air sampler is connected via a one-way valve inside the device to the disposable viral antigen reactor. The disposable air sample cracker contains air sample virus antigen detection cracking liquid which is loaded in advance, and carries out cracking reaction on the air sample under set conditions. When the cracking reaction is completed, the one-way valve in the device is opened, and the cracked air sample is fed into the disposable viral antigen reactor under the action of gas pressure.
4. The disposable viral antigen reactor of claim 1 wherein one end is connected to the disposable air sample cracker through a one-way valve in the device and is connected to the antigen reactor physical status sensing probe by non-direct contact. The virus antigen reactor contains virus antigen preset on medium carrier capable of changing physical state, after the cracked air sample lysate is fed into the disposable virus antigen reactor, the air sample and virus antigen are made to produce immune reaction, and because the immune reaction is specific reaction to virus, no false alarm will occur when the air sample contains no detected virus. When the air sample contains the detected virus, the generated antigen immune reaction can cause the physical state change of the antigen carrier medium (the physical state change comprises but is not limited to the color and the resistance of the antigen medium carrier in the virus antigen reactor, and the like), the change can be received by the physical state change detector sensing probe of the virus antigen reactor which is not directly contacted with the virus antigen reactor and is transmitted to the central control MCU (microprocessor) by the probe, and when the change reaches a set threshold value, the MCU (microprocessor) is triggered to send out detection warning and other processing actions.
5. An MCU (microprocessor) and peripheral circuitry controlled by the apparatus of claim 1, wherein the following functions are included but not limited to:
opening and closing each one-way valve according to the program setting;
pushing the disposable air sample cracker and the disposable viral antigen reactor to automatically replace a new cracker and a new reactor according to the program setting;
receiving sensing probe data and judging according to a program;
self-cleaning the internal pipeline according to program control;
sending a sample detection virus detection warning and virus concentration data set according to a program to a control system center;
sending the using state data of the disposable cracker and the disposable reactor to a control system center and providing warning needed to be supplemented;
providing real-time video information when the virus detection exceeds a threshold value to a deployment control system center;
providing the abnormal information of the detection device to a control system center;
6. the communication circuit of claim 1, further comprising a communication device integrated with the peripheral circuit, the communication device being capable of communicating with the administration system center via wired or wireless communication.
7. The portrait acquisition system of claim 1, comprising a 24-hour video capture recording function within range of the detection device and a programmed time period during which a virus detection exceeds a threshold. And acquiring the portrait within a time period set by a program when the threshold value is reached.
FIG. 1 is a schematic diagram of a detection apparatus, which is illustrated as follows:
1 is air sampler, 2 is disposable air sample cracker, 3 is disposable virus antigen reactor, 4 is virus antigen reactor physical state change detector, 5 is control MCU and peripheral circuit, 6 is communication module, 7 is video module, 8 to 10 are controllable one-way valve, 11 is outside air
Detailed Description
1. Referring to the drawings, when external air 11 is sucked into the air sampler 1 through a micro-motor and a fan in the air sampler 1, the process is controlled by a check valve 1 controlled by a micro-controller unit (microprocessor) and opens a check valve 8 at set time intervals, and the sucked air is subjected to speed reduction regulation in an internal air duct after an internal fan in the air sampler 1 and is sent to an air sample reservoir in the air sampler 1 for temporary storage. The sample reservoir contains a solution of pre-filled activators, dispersants, etc. for subsequent sample lysis. When the system reaches the set sampling time, the controlled one-way valve 9 is opened and the air sample solution is pushed into figure 2 (disposable air sample cracker) using the internal pressure.
2. Two ends of a 2 (disposable air sample cracker) in the attached figure comprise sealing plugs which are made of flexible materials such as silica gel and fall in a single direction, and when the 2 is added into the device, the sealing plugs are pushed into the disposable air sample cracker under the action of the top heads preset in the front end pipeline and the rear end pipeline, so that the inlet and the outlet of the liquid phase flow of the sample cracker are opened. The disposable air sample cracker is preset with relevant enzyme system and other supplementary liquid for cracking virus sample, and if necessary, the temperature and vibration of the sample cracker are controlled based on the cracking condition. After completion of the lysis reaction within the set lysis time, the controlled one-way valve 10 is opened and the sample lysate is injected 3 (disposable viral antigen reactor) by internal air pressure.
3. In the attached figure 3 (disposable viral antigen reactor) the liquid phase inlet end contains a one-way falling sealing plug made of flexible materials such as silica gel, when 3 is added into the device, the sealing plug is pushed to fall into the disposable viral antigen reactor under the action of a top head preset in a front end pipeline, so that the liquid phase inlet of the reactor is opened. The disposable virus antigen reactor 3 comprises a preset sample liquid container, a sample liquid flowing substrate and other devices. The sample liquid is driven by capillary action, electrophoresis action or mobile phase driving action, etc. to flow from one end of the matrix to the other end of the matrix according to a set flow rate on the flowing matrix. The sample liquid flowing matrix is provided with a relevant antigen reaction area of virus to be detected in advance at a certain position, the antibody of the virus to be detected is fixed on the reaction area in advance, after the sample liquid flows to the antigen reaction area, if the sample liquid contains the antigen of specific virus, the sample liquid and the antibody in the antigen reaction area are subjected to immunoreaction, and the reaction can change the conductivity or the color of the antigen reaction area. Whereas if no antigen of a specific virus is contained, the conductivity or color change of the reaction region is within a controlled range. The result of the change in the electric conductivity or color in the antigen reaction region after the completion of the reaction is measured by 4 (reactor physical state change sensor) in the drawing to judge whether or not the virus to be detected is contained in the air sample.
4. The 2 (disposable air sample cracker) and the 3 (disposable viral antigen reactor) are both designed to be disposable, and after each detection is completed, the internal pipeline of the device is automatically replaced and cleaned through a replacement system arranged in the device.
5. Fig. 5 in the drawing is a device control system, and its main components are an MCU (microprocessor) for control and peripheral circuits.
6. Fig. 6 shows a built-in communication module of the device, which is used for communicating with a social virus control center in a wired or wireless manner, including the operation state of the device and the occurrence of virus detection warning.
7. Fig. 7 in the accompanying drawings is a built-in video module of the device, which is used for video acquisition of the device site before and after a virus detection warning occurs, and provides help for effectively finding out virus carrying personnel.
Claims (1)
1. The invention relates to a real-time detection device for viruses spread by convection in an air environment, which is characterized by comprising an ambient air sampler, a disposable air sample cracker, a disposable virus antigen reactor and a virus antigen reactor physical state change detector.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202210201049.8A CN114594208A (en) | 2022-03-07 | 2022-03-07 | Real-time detection device for virus spread to epidemic disease in air environment |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202210201049.8A CN114594208A (en) | 2022-03-07 | 2022-03-07 | Real-time detection device for virus spread to epidemic disease in air environment |
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| Publication Number | Publication Date |
|---|---|
| CN114594208A true CN114594208A (en) | 2022-06-07 |
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202210201049.8A Pending CN114594208A (en) | 2022-03-07 | 2022-03-07 | Real-time detection device for virus spread to epidemic disease in air environment |
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-
2022
- 2022-03-07 CN CN202210201049.8A patent/CN114594208A/en active Pending
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