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CN114577731A - A test tube composition detection device and method - Google Patents

A test tube composition detection device and method Download PDF

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CN114577731A
CN114577731A CN202210136790.0A CN202210136790A CN114577731A CN 114577731 A CN114577731 A CN 114577731A CN 202210136790 A CN202210136790 A CN 202210136790A CN 114577731 A CN114577731 A CN 114577731A
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blood
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CN114577731B (en
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江胜标
黄振有
李运奇
赖鹏飞
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Shenzhen Aikang Medtech Co Ltd
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    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
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    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
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Abstract

The invention discloses a test tube component detection device and a test tube component detection method. According to the scheme, the sample signal values are obtained by utilizing the ultra-strong penetrating power of laser, the signal values of laser penetrating blood in different position states are obtained by utilizing the rotating device, the processor is matched with the signal threshold value range conditions under each blood quality classification of the preset signal threshold value group through the first signal value, the second signal value and the third signal value in different position states, the quality condition of a target sample is analyzed according to the matching result, the bar code pasting on the surface of the sample test tube is fully considered, the signal values in different position states are obtained by utilizing the rotating device based on the bar code pasting state, the blood quality condition is comprehensively judged by utilizing the signal values in different position states, and the blood quality identification accuracy is high.

Description

一种试管成分检测装置及方法A test tube composition detection device and method

技术领域technical field

本发明涉及医疗器械技术领域,特别是一种试管成分检测装置及方法。The invention relates to the technical field of medical devices, in particular to a test tube component detection device and method.

背景技术Background technique

医学临床检验过程包括分析前、分析中、分析后3个阶段,分析前误差占实验室总误差的70%左右,因此,确保样本质量合格是检验结果准确可靠的前提。The medical clinical inspection process includes three stages: before analysis, during analysis, and after analysis. The error before analysis accounts for about 70% of the total laboratory error. Therefore, ensuring that the sample quality is qualified is the premise of accurate and reliable inspection results.

目前,实验室主要是通过检验人员在前台接收样本时或人工上仪器检测前检查样本是否使用正确的采血管、样本量是否过多或过少、血清或血浆占比是否过多、样本是否离心、样本是否溶血黄疸脂血、是否有凝块或纤维丝等,通过人工来评估样本质量是否合格,这对于工作人员而言工作量较大,且人工检测效率较低,出错率也较高,为此,有一部分实验室引入了连接有样本前处理系统的流水线系统,自动实现样本的识别、离心、开盖等操作,一定程度上缩减了人工干预环节,其中,对于样本质量的识别,现有技术中通常采用摄像机拍照识别的方式,通过将拍摄的样本照片与预置的标准样本照片进行比对,进而分析样本质量情况,但是,对于该方案,由于样本试管所贴条码的粘贴位置可能存在一定的个体或批量差异,因此极大地影响了血液质量的识别。At present, the laboratory mainly checks whether the sample uses the correct blood collection tube, whether the sample volume is too much or too little, whether the proportion of serum or plasma is too much, and whether the sample is centrifuged when receiving the sample at the front desk or before manual testing. , Whether the sample is hemolytic, icteric, fatty, clot or fibrous, etc., the quality of the sample is evaluated manually, which is a large workload for the staff, and the manual detection efficiency is low and the error rate is high. To this end, some laboratories have introduced an assembly line system connected with a sample preprocessing system to automatically realize operations such as sample identification, centrifugation, and cap opening, reducing manual intervention to a certain extent. Among them, the identification of sample quality is now In the prior art, the method of camera photo identification is usually adopted, and the sample quality is analyzed by comparing the captured sample photo with the preset standard sample photo. There are certain individual or batch differences, thus greatly affecting the identification of blood quality.

发明内容SUMMARY OF THE INVENTION

本发明为了克服现有技术存在的不足,提供一种试管成分检测装置及方法。In order to overcome the shortcomings of the prior art, the present invention provides a test tube component detection device and method.

本发明通过以下技术方案来实现:The present invention realizes through the following technical solutions:

一种试管成分检测装置,其特征在于,包括旋转装置、激光束发射器、激光束接收器和处理器,所述激光束发射器与所述激光束接收器相对设置,所述激光束接收器包括光电二极管和信号放大器;A test tube composition detection device, characterized in that it includes a rotating device, a laser beam transmitter, a laser beam receiver and a processor, wherein the laser beam transmitter is arranged opposite to the laser beam receiver, and the laser beam receiver is arranged opposite to the laser beam receiver. Including photodiodes and signal amplifiers;

所述旋转装置用于承载样本试管并对样本试管进行旋转;The rotating device is used to carry the sample test tube and rotate the sample test tube;

所述激光束发射器用于发射透射血液样本的激光束;the laser beam transmitter is used for emitting a laser beam that transmits the blood sample;

所述激光束接收器用于接收穿透所述血液样本的激光束,并根据接收的激光生成信号值;the laser beam receiver is used for receiving a laser beam penetrating the blood sample, and generating a signal value according to the received laser light;

所述处理器用于根据所述信号值分析判断血液质量分级。The processor is configured to analyze and judge the blood quality classification according to the signal value.

进一步的,其特征在于,所述旋转装置设置于所述激光束发射器和所述激光束接收器之间以用于将承载的样本试管在所述激光束发射器的激光发射区域和所述激光束接收器的激光接收区域之间进行旋转。Further, it is characterized in that, the rotating device is arranged between the laser beam transmitter and the laser beam receiver, so as to place the carried sample test tube between the laser emission area of the laser beam transmitter and the laser beam receiver. The laser beam receiver is rotated between the laser receiving areas.

进一步的,其特征在于,所述处理器包括数据库系统和分析系统;Further, it is characterized in that the processor includes a database system and an analysis system;

所述数据库系统用于存储不同质量分级的血液的信号阈值组;The database system is used to store signal threshold groups of blood of different quality grades;

所述分析系统用于分析血液质量分级。The analysis system is used to analyze blood quality grades.

进一步的,所述数据库系统的搭建方法,包括:Further, the construction method of the database system includes:

设定血液质量分级数量和分级类型;Set the number and type of blood quality grading;

根据血液质量分级情况,预先选取N个已知血液质量分级的样本,并获取所述N个已知血液质量分级的样本的信号值组,其中,N为不小于血液质量分级数量的自然数,且所述N个已知血液质量分级的样本应覆盖各设定的血液质量分级;According to the blood quality grading situation, N samples of known blood quality grading are preselected, and a signal value group of the N samples of known blood quality grading is obtained, where N is a natural number not less than the number of blood quality grading, and The N samples of known blood quality grades should cover each set blood quality grade;

根据所获取的N个已知血液质量分级的样本的信号值组,设定不同血液质量分级状态下的信号阈值组。According to the acquired signal value groups of N samples with known blood quality gradings, signal threshold value groups under different blood quality grading states are set.

进一步的,所述样本试管上粘贴有条码,所述条码与样本试管之间留有一缝隙,所述信号值组包括第一信号值、第二信号值和第三信号值,所述N 个已知血液质量分级的样本的信号值组的获取方法,包括:Further, a barcode is pasted on the sample test tube, and a gap is left between the barcode and the sample test tube, and the signal value group includes a first signal value, a second signal value and a third signal value, and the N have been The method for obtaining the signal value group of the sample with known blood quality grading includes:

所述旋转装置带动粘贴有条码的样本试管进行旋转;The rotating device drives the sample test tube pasted with the barcode to rotate;

获取激光束从一侧条码发射透射血液样本,并从另一侧条码射出时的第一信号值;Obtain the first signal value when the laser beam emits the transmitted blood sample from the barcode on one side and shoots out from the barcode on the other side;

获取激光束从一侧条码发射透射血液样本,并从缝隙射出时的第二信号值;Obtain the second signal value when the laser beam transmits the blood sample from one side of the barcode and exits from the slit;

获取激光束从缝隙发射透射血液样本,并从另一侧条码射出时的第三信号值。Obtain the third signal value when the laser beam is emitted from the slit to transmit the blood sample and emitted from the barcode on the other side.

进一步的,所述第一信号值小于所述第二信号值,所述第二信号值小于所述第三信号值。Further, the first signal value is smaller than the second signal value, and the second signal value is smaller than the third signal value.

进一步的,所述血液质量分级包括正常、轻度质量异常和重度质量异常,所述信号阈值组包括第一正常信号阈值、第二正常信号阈值、第三正常信号阈值、第一轻度信号阈值、第二轻度信号阈值、第三轻度信号阈值、第一重度信号阈值、第二重度信号阈值和第三重度信号阈值,所述信号阈值组的获取方法,包括:Further, the blood quality classification includes normal, mild abnormality and severe abnormality, and the signal threshold group includes a first normal signal threshold, a second normal signal threshold, a third normal signal threshold, and a first mild signal threshold. , the second mild signal threshold, the third mild signal threshold, the first severe signal threshold, the second severe signal threshold and the third severe signal threshold, the method for obtaining the signal threshold group includes:

设定正常血液的已知样本数量为n1,设定轻度质量异常的已知样本数量为n2,设定重度质量异常的已知样本数量为n3,其中,n1+n2+n3=N,且n1、 n2和n3均为不小于1的自然数;The number of known samples of normal blood is set to n 1 , the number of known samples of mild abnormal quality is set to n 2 , and the number of known samples of severe abnormal quality is set to n 3 , where n 1 +n 2 + n 3 =N, and n 1 , n 2 and n 3 are all natural numbers not less than 1;

按照前述方法分别获取并计算n1个正常血液的已知样本的第一信号值、第二信号值和第三信号值的平均值,设定n1个正常血液的已知样本的第一信号值的平均值为第一正常信号阈值,设定n1个正常血液的已知样本的第二信号值的平均值为第二正常信号阈值,设定n1个正常血液的已知样本的第三信号值的平均值为第三正常信号阈值;Obtain and calculate the average value of the first signal value, the second signal value and the third signal value of n 1 known samples of normal blood according to the aforementioned method, and set the first signal of n 1 known samples of normal blood The average value of the values is the first normal signal threshold, and the average value of the second signal values of n 1 known samples of normal blood is set as the second normal signal threshold, and the second normal signal threshold of n 1 known samples of normal blood is set. The average value of the three signal values is the third normal signal threshold;

按照前述方法分别获取并计算n2个轻度质量异常的已知样本的第一信号值、第二信号值和第三信号值的平均值,设定n2个轻度质量异常的已知样本的第一信号值的平均值为第一轻度信号阈值,设定n2个轻度质量异常的已知样本的第二信号值的平均值为第二轻度信号阈值,设定n2个轻度质量异常的已知样本的第三信号值的平均值为第三轻度信号阈值;Acquire and calculate the average value of the first signal value, the second signal value and the third signal value of n 2 known samples with mild abnormal quality respectively according to the aforementioned method, and set n 2 known samples with mild abnormal quality The average value of the first signal values is the first light signal threshold, and the average value of the second signal values of n 2 known samples with mild abnormality is set as the second light signal threshold, and n 2 are set The average value of the third signal values of the known samples with mild quality abnormality is the third mild signal threshold;

按照前述方法分别获取并计算n3个重度质量异常的已知样本的第一信号值、第二信号值和第三信号值的平均值,设定n3个重度质量异常的已知样本的第一信号值的平均值为第一重度信号阈值,设定n3个重度质量异常的已知样本的第二信号值的平均值为第二重度信号阈值,设定n3个重度质量异常的已知样本的第三信号值的平均值为第三重度信号阈值。Acquire and calculate the average value of the first signal value, the second signal value and the third signal value of n 3 known samples with severe abnormal quality respectively according to the aforementioned method, and set the first signal value of n 3 known samples with severe abnormal quality The average value of a signal value is the first severe signal threshold, the average value of the second signal values of n 3 known samples with severe abnormal quality is set as the second severe signal threshold, and the n 3 known samples with severe abnormal quality are set as the second severe signal threshold. The average value of the third signal values of the known samples is the third severe signal threshold.

进一步的,设定所述第一正常信号阈值、第二正常信号阈值、第三正常信号阈值、第一轻度信号阈值、第二轻度信号阈值、第三轻度信号阈值、第一重度信号阈值、第二重度信号阈值和第三重度信号阈值分别为N1、N2、N3、M1、M2、M3、H1、H2和H3,其中,600≤N1≤700,1500≤N2≤1800,2500≤ N3≤6000,500≤M1≤650,800≤M2≤1300,1200≤M3≤5500,500≤H1≤600, 650≤H2≤750,700≤H3≤1200。Further, set the first normal signal threshold, the second normal signal threshold, the third normal signal threshold, the first mild signal threshold, the second mild signal threshold, the third mild signal threshold, and the first severe signal The threshold, the second severe signal threshold and the third severe signal threshold are respectively N 1 , N 2 , N 3 , M 1 , M 2 , M 3 , H 1 , H 2 and H 3 , where 600≤N 1 ≤ 700, 1500≤N 2 ≤1800, 2500≤N 3 ≤6000, 500≤M 1 ≤650, 800≤M 2 ≤1300, 1200≤M 3 ≤5500, 500≤H 1 ≤600, 650≤H 2 ≤750 , 700≤H 3 ≤1200.

进一步的,所述分析系统分析血液质量分级的方法包括:Further, the method for analyzing the blood quality classification by the analysis system includes:

设定目标样本的第一信号值、第二信号值和第三信号值分别为P1、P2 和P3;Set the first signal value, the second signal value and the third signal value of the target sample as P1, P2 and P3 respectively;

当600≤P1≤700,且1500≤P2≤1800,且2500≤P3≤6000时,所述目标样本为正常样本;When 600≤P 1 ≤700, and 1500≤P 2 ≤1800, and 2500≤P 3 ≤6000, the target sample is a normal sample;

当500≤P1≤650,且800≤P2≤1300,且1200≤P3≤5500,所述目标样本为轻度质量异常的样本;When 500≤P 1 ≤650, and 800≤P 2 ≤1300, and 1200≤P 3 ≤5500, the target sample is a sample with mild abnormal quality;

当500≤P1≤600,且650≤P2≤750,且700≤P3≤1200,所述目标样本为重度质量异常的样本。When 500≤P 1 ≤600, and 650≤P 2 ≤750, and 700≤P 3 ≤1200, the target sample is a sample with severe abnormal quality.

一种试管成分检测方法,具体包括:A test tube component detection method, specifically comprising:

将装载有目标样本的样本试管放置于所述旋转装置中;placing the sample tube loaded with the target sample in the rotating device;

所述旋转装置驱动对样本试管进行旋转;The rotating device drives the sample test tube to rotate;

所述激光束发射器发射透射血液样本的激光束;the laser beam transmitter emits a laser beam that transmits the blood sample;

所述激光束接收器接收穿透所述血液样本的激光束,并根据接收的激光生成第一信号值、第二信号值和第三信号值;the laser beam receiver receives a laser beam penetrating the blood sample, and generates a first signal value, a second signal value and a third signal value according to the received laser light;

所述激光束接收器将所生成的第一信号值、第二信号值和第三信号值反馈至处理器;the laser beam receiver feeds back the generated first signal value, second signal value and third signal value to the processor;

所述处理器将所接收到的目标样本的第一信号值、第二信号值和第三信号值与所述信号阈值组的各血液质量分级下的信号阈值范围条件进行匹配;The processor matches the received first signal value, second signal value and third signal value of the target sample with signal threshold range conditions under each blood quality classification of the signal threshold group;

所述处理器根据匹配结果分析目标样本的质量情况。The processor analyzes the quality of the target sample according to the matching result.

结合本发明的结构特点,与现有技术相比,本发明所提供的一种试管成分检测装置及方法,试管成分检测装置包括旋转装置、激光束发射器、激光束接收器和处理器,所述激光束发射器与所述激光束接收器相对设置,所述旋转装置用于承载样本试管并对样本试管进行旋转,所述激光束发射器用于发射透射血液样本的激光束,所述激光束接收器用于接收穿透所述血液样本的激光束,并根据接收的激光生成信号值,所述处理器用于根据所述信号值分析判断血液质量分级。该方案利用激光的超强穿透力获取样本信号值,并利用旋转装置来获取不同位置状态下激光穿透血液的信号值,处理器通过不同位置状态下的第一信号值、第二信号值和第三信号值与预设的信号阈值组的各血液质量分级下的信号阈值范围条件进行匹配并根据匹配结果分析目标样本的质量情况,对于该方案,考虑了样本试管表面的条码粘贴,并基于条码粘贴状态同时利用旋转装置来获取激光从一侧条码进另一侧条码射出、从一侧条码进从缝隙射出以及从缝隙进从另一侧条码射出等不同位置状态下的信号值,利用不同位置状态下的信号值进行综合判定血液质量情况,血液质量识别准确度高,有效的解决了现有技术中由于样本试管所贴条码的粘贴位置可能存在一定的个体或批量差异导致的影响血液质量的识别准确度的问题。Combined with the structural features of the present invention, compared with the prior art, the present invention provides a test tube composition detection device and method. The test tube composition detection device includes a rotating device, a laser beam transmitter, a laser beam receiver and a processor, so The laser beam transmitter is arranged opposite to the laser beam receiver, the rotating device is used to carry the sample test tube and rotate the sample test tube, the laser beam transmitter is used to emit a laser beam that transmits the blood sample, the laser beam The receiver is used for receiving the laser beam penetrating the blood sample, and generating a signal value according to the received laser light, and the processor is used for analyzing and determining the blood quality classification according to the signal value. The scheme uses the super penetrating power of the laser to obtain the signal value of the sample, and uses the rotating device to obtain the signal value of the laser penetrating the blood in different positions. and the third signal value are matched with the signal threshold range conditions under each blood quality classification of the preset signal threshold group, and the quality of the target sample is analyzed according to the matching result. For this scheme, the barcode on the surface of the sample test tube is considered, and Based on the bar code pasting state, the rotating device is used to obtain the signal values of the laser from one side of the bar code to the other side of the bar code, from one side of the bar code to the gap, and from the gap to the other side of the bar code. The signal values in different positions are used to comprehensively determine the blood quality, and the blood quality recognition accuracy is high, which effectively solves the problem in the prior art, which may be caused by individual or batch differences in the pasting position of the barcode attached to the sample test tube. The problem of quality identification accuracy.

附图说明Description of drawings

为了更清楚地说明本申请实施例或现有技术中的技术方案,下面将对实施例中所需要使用的附图作简单地介绍,显而易见地,下面描述中的附图仅仅是本发明中记载的一些实施例,对于本领域普通技术人员来讲,还可以根据这些附图获得其他的附图。In order to more clearly illustrate the embodiments of the present application or the technical solutions in the prior art, the accompanying drawings required in the embodiments will be briefly introduced below. Obviously, the accompanying drawings in the following description are only described in the present invention. For some of the embodiments, those of ordinary skill in the art can also obtain other drawings according to these drawings.

图1为本发明所述的一种试管成分检测装置的示意图。FIG. 1 is a schematic diagram of a test tube component detection device according to the present invention.

其中,1-旋转装置,2-样本试管,3-条码,4-缝隙。Among them, 1-rotating device, 2-sample test tube, 3-barcode, 4-slot.

具体实施方式Detailed ways

为使本发明的目的、技术方案和优点更加清楚,下面将结合附图对本发明的实施方式作进一步地描述。显然,所描述的实施例仅仅是本发明一部分实施例,而不是全部的实施例。基于本发明中的实施例,本领域普通技术人员在没有做出创造性劳动前提下所获得的所有其他实施例,都属于本发明保护的范围。In order to make the objectives, technical solutions and advantages of the present invention clearer, the embodiments of the present invention will be further described below with reference to the accompanying drawings. Obviously, the described embodiments are only some, but not all, embodiments of the present invention. Based on the embodiments of the present invention, all other embodiments obtained by those of ordinary skill in the art without creative efforts shall fall within the protection scope of the present invention.

需要说明,本发明实施例中所有方向性指示(如上、下、左、右、前、后等)仅用于解释在某一特定姿态下各部件之间的相对位置关系、运动情况等,如果该特定姿态发生改变时,则该方向性指示也相应随之改变。It should be noted that all the directional indications (such as up, down, left, right, front, back, etc.) in the embodiments of the present invention are only used to explain the relative positional relationship, movement situation, etc. When the specific posture changes, the directional indication also changes accordingly.

如图1所示,一种试管成分检测装置,包括旋转装置1、激光束发射器、激光束接收器和处理器,所述激光束发射器与所述激光束接收器相对设置,所述激光束接收器包括光电二极管和信号放大器;As shown in FIG. 1, a test tube composition detection device includes a rotating device 1, a laser beam transmitter, a laser beam receiver and a processor, the laser beam transmitter and the laser beam receiver are arranged opposite to the laser beam receiver, and the laser beam The beam receiver includes a photodiode and a signal amplifier;

所述旋转装置1设置于所述激光束发射器和所述激光束接收器之间以用于将承载的样本试管2在所述激光束发射器的激光发射区域和所述激光束接收器的激光接收区域之间进行360°旋转;需要说明的是,图1所示仅为示意图,不代表旋转装置的实际结构特征,只要具备旋转功能的机构装置均落入本发明的保护范围;The rotating device 1 is arranged between the laser beam transmitter and the laser beam receiver for placing the carried sample test tube 2 in the laser emission area of the laser beam transmitter and the laser beam receiver. 360° rotation is performed between the laser receiving areas; it should be noted that the diagram shown in FIG. 1 is only a schematic diagram, and does not represent the actual structural characteristics of the rotating device, as long as the mechanism device with the rotating function falls within the protection scope of the present invention;

所述激光束发射器用于发射透射血液样本的激光束;需要说明的是,所述激光发射器可以为多个激光发射装置形成的激光束发射器,也可以为单束激光经过光路分路装置分散成多束激光形成的激光束发射器,均落入本发明的保护范围;The laser beam transmitter is used to emit a laser beam that transmits the blood sample; it should be noted that the laser beam transmitter can be a laser beam transmitter formed by multiple laser emitting devices, or can be a single laser beam passing through an optical path splitting device The laser beam emitters formed by dispersing into multiple laser beams all fall into the protection scope of the present invention;

所述激光束接收器用于接收穿透所述血液样本的激光束,并根据接收的激光生成信号值;需要说明的是,所述信号值可以为电流值、电压值、波长值等,均落入本发明的保护范围。The laser beam receiver is used to receive the laser beam penetrating the blood sample, and generate a signal value according to the received laser light; it should be noted that the signal value can be a current value, a voltage value, a wavelength value, etc. into the protection scope of the present invention.

所述处理器用于根据所述信号值分析判断血液质量分级。The processor is configured to analyze and judge the blood quality classification according to the signal value.

在一实施例中,所述处理器包括数据库系统和分析系统,所述数据库系统用于存储不同质量分级的血液的信号阈值组,所述分析系统用于分析血液质量分级,其中,所述数据库系统的搭建方法,包括:In one embodiment, the processor includes a database system for storing signal threshold sets of blood of different quality grades, and an analysis system for analyzing the blood quality grades, wherein the database The system construction method, including:

设定血液质量分级数量和分级类型,在该实施例中,所述血液质量分级包括3个分级,分别为正常、轻度质量异常和重度质量异常,其中,轻度质量异常和重度质量异常分别为轻度脂血和重度脂血的血液样本;The blood quality classification quantity and classification type are set. In this embodiment, the blood quality classification includes 3 classifications, which are normal, mildly abnormal, and severely abnormal. Blood samples for mild lipidemia and severe lipidemia;

根据血液质量分级情况,预先选取12个已知血液质量分级的样本,其中,这12个已知血液质量分级的样本覆盖了所设定的3个不同分级的血液质量,在该实施例中,所述12个已知血液质量分级的样本中有3个正常样本,4个轻度脂血的样本,以及5个重度脂血的样本,装载样本的样本试管2上粘贴有条码3,条码用于储存样本信息,比如检测项目信息、患者信息等等,所述条码3 与样本试管2之间留有一缝隙4,如图1所示,通过以下S1~S4的方法获取所述 12个已知血液质量分级的样本的信号值组,其中,所述信号值组包括第一信号值、第二信号值和第三信号值:According to the blood quality grading situation, 12 samples of known blood quality grading are pre-selected, wherein these 12 samples of known blood quality grading cover the blood quality of 3 different gradings set. In this embodiment, Among the 12 samples with known blood quality grades, there are 3 normal samples, 4 samples with mild lipemia, and 5 samples with severe lipemia. The sample tube 2 that loads the samples has barcode 3 pasted, and the barcode is used for For storing sample information, such as test item information, patient information, etc., there is a gap 4 between the barcode 3 and the sample test tube 2, as shown in FIG. A set of signal values for a blood quality graded sample, wherein the set of signal values includes a first signal value, a second signal value, and a third signal value:

S1:所述旋转装置1带动粘贴有条码3的样本试管2进行360°旋转;S1: the rotating device 1 drives the sample test tube 2 pasted with the barcode 3 to rotate 360°;

S2:所述激光束发射器发射透射血液样本的激光束,在旋转装置带动样本试管旋转过程中,所述激光束接收器接收从一侧条码发射透射血液样本并从另一侧条码射出时的穿透所述血液样本的激光束并转换成数字的信号值作为第一信号值;S2: The laser beam transmitter emits a laser beam that transmits the blood sample. During the rotation of the sample test tube driven by the rotating device, the laser beam receiver receives the transmitted blood sample from the barcode on one side and shoots out from the barcode on the other side. A laser beam that penetrates the blood sample and is converted into a digital signal value as a first signal value;

S3:所述激光束发射器发射透射血液样本的激光束,在旋转装置带动样本试管旋转过程中,所述激光束接收器接收从一侧条码发射透射血液样本并从缝隙4射出时的穿透所述血液样本的激光束并转换成数字的信号值作为第二信号值;S3: The laser beam transmitter emits a laser beam that transmits the blood sample. During the rotation of the sample test tube driven by the rotating device, the laser beam receiver receives the penetrating blood sample transmitted from the bar code on one side and emitted from the slit 4. The laser beam of the blood sample is converted into a digital signal value as the second signal value;

S4:所述激光束发射器发射透射血液样本的激光束,在旋转装置带动样本试管旋转过程中,所述激光束接收器接收从缝隙发射透射血液样本并从另一侧条码射出时的穿透所述血液样本的激光束并转换成数字的信号值作为第三信号值;S4: The laser beam transmitter emits a laser beam that transmits the blood sample. During the rotation of the sample test tube driven by the rotating device, the laser beam receiver receives the transmitted blood sample from the slit and shoots out from the barcode on the other side. The laser beam of the blood sample is converted into a digital signal value as a third signal value;

其中,通过实验研究,所述第一信号值小于所述第二信号值,所述第二信号值小于所述第三信号值。Wherein, through experimental research, the first signal value is smaller than the second signal value, and the second signal value is smaller than the third signal value.

根据上述所获取的12个已知血液质量分级的样本的信号值组,通过以下S5~S8的方法设定各血液质量分级状态下的信号阈值组,所述信号阈值组包括第一正常信号阈值、第二正常信号阈值、第三正常信号阈值、第一轻度信号阈值、第二轻度信号阈值、第三轻度信号阈值、第一重度信号阈值、第二重度信号阈值和第三重度信号阈值:According to the obtained signal value groups of 12 samples with known blood quality grading, the following methods S5 to S8 are used to set the signal threshold value group in each blood quality grading state, and the signal threshold value group includes the first normal signal threshold value , Second Normal Signal Threshold, Third Normal Signal Threshold, First Light Signal Threshold, Second Light Signal Threshold, Third Light Signal Threshold, First Severe Signal Threshold, Second Severe Signal Threshold, and Third Severe Signal Threshold Signal Threshold:

S5:正常血液的已知样本数量为3,轻度脂血的已知样本数量为4,重度脂血的已知样本数量为5;S5: The number of known samples of normal blood is 3, the number of known samples of mild lipidemia is 4, and the number of known samples of severe lipidemia is 5;

S6:按照前述S1~S4的方法分别获取并计算3个正常血液的已知样本的第一信号值、第二信号值和第三信号值的平均值,将所述3个正常血液的已知样本的第一信号值的平均值为第一正常信号阈值N1,将所述3个正常血液的已知样本的第二信号值的平均值为第二正常信号阈值N2,将所述3个正常血液的已知样本的第三信号值的平均值为第三正常信号阈值N3S6: Acquire and calculate the average value of the first signal value, the second signal value, and the third signal value of the three known samples of normal blood according to the methods of S1 to S4, respectively, and calculate the known value of the three normal blood samples. The average value of the first signal values of the samples is the first normal signal threshold value N 1 , the average value of the second signal values of the three known samples of normal blood is the second normal signal threshold value N 2 , and the 3 The average value of the third signal values of the known samples of normal blood is the third normal signal threshold N 3 ;

S7:按照前述S1~S4的方法分别获取并计算4个轻度质量异常的已知样本的第一信号值、第二信号值和第三信号值的平均值,将所述4个轻度脂血的已知样本的第一信号值的平均值为第一轻度信号阈值M1,将所述4个轻度脂血的已知样本的第二信号值的平均值为第二轻度信号阈值M2,将所述4个轻度脂血的已知样本的第三信号值的平均值为第三轻度信号阈值M3S7: Acquire and calculate the average value of the first signal value, the second signal value, and the third signal value of the four known samples with mildly abnormal quality respectively according to the methods of S1 to S4, The average value of the first signal values of the known samples of blood is the first mild signal threshold M 1 , and the average value of the second signal values of the 4 known samples of mild lipidemia is the second mild signal threshold value M 2 , the average value of the third signal values of the 4 known samples of mild lipidemia is the third mild signal threshold value M 3 ;

S8:按照前述S1~S4的方法分别获取并计算5个重度脂血的已知样本的第一信号值、第二信号值和第三信号值的平均值,将所述5个重度脂血的已知样本的第一信号值的平均值为第一重度信号阈值H1,将所述5个重度脂血的已知样本的第二信号值的平均值为第二重度信号阈值H2,将所述5个重度脂血的已知样本的第三信号值的平均值为第三重度信号阈值H3S8: Acquire and calculate the average value of the first signal value, the second signal value and the third signal value of the five known samples of severe lipidemia according to the methods of S1 to S4, respectively, The average value of the first signal values of the known samples is the first severe signal threshold H 1 , the average value of the second signal values of the five known samples of severe lipidemia is the second severe signal threshold H 2 , and the The average value of the third signal values of the five known samples of severe lipidemia is the third severe signal threshold H 3 ;

在一实施例中,600≤N1≤700,1500≤N2≤1800,2500≤N3≤6000, 500≤M1≤650,800≤M2≤1300,1200≤M3≤5500,500≤H1≤600,650≤H2≤750,700≤H3≤1200。In an embodiment, 600≤N 1 ≤700, 1500≤N 2 ≤1800, 2500≤N 3 ≤6000, 500≤M 1 ≤650, 800≤M 2 ≤1300, 1200≤M 3 ≤5500, 500≤ H 1 ≤ 600, 650 ≤ H 2 ≤ 750, 700 ≤ H 3 ≤ 1200.

在一实施例中,所述分析系统分析血液质量分级的方法包括:In one embodiment, the method for analyzing blood quality grading by the analysis system includes:

设定目标样本的第一信号值、第二信号值和第三信号值分别为P1、P2 和P3;Set the first signal value, the second signal value and the third signal value of the target sample as P1, P2 and P3 respectively;

当600≤P1≤700,且1500≤P2≤1800,且2500≤P3≤6000时,所述目标样本为正常样本;When 600≤P 1 ≤700, and 1500≤P 2 ≤1800, and 2500≤P 3 ≤6000, the target sample is a normal sample;

当500≤P1≤650,且800≤P2≤1300,且1200≤P3≤5500,所述目标样本为轻度脂血的样本;When 500≤P 1 ≤650, and 800≤P 2 ≤1300, and 1200≤P 3 ≤5500, the target sample is a sample with mild lipidemia;

当500≤P1≤600,且650≤P2≤750,且700≤P3≤1200,所述目标样本为重度脂血的样本。When 500≦P 1 ≦600, and 650≦P 2 ≦750, and 700≦P 3 ≦1200, the target sample is a sample with severe lipidemia.

以下为9个目标样本的分析结果,其中,如前述实施例所述,第一信号值为所述激光束接收器接收从一侧条码发射透射血液样本并从另一侧条码射出时的穿透所述血液样本的激光束并转换成数字的信号值,第二信号值为所述激光束接收器接收从一侧条码发射透射血液样本并从缝隙4射出时的穿透所述血液样本的激光束并转换成数字的信号值,第三信号值为所述激光束接收器接收从缝隙发射透射血液样本并从另一侧条码射出时的穿透所述血液样本的激光束并转换成数字的信号值:The following are the analysis results of 9 target samples, wherein, as described in the previous embodiment, the first signal value is the penetration of the laser beam receiver when the transmitted blood sample is transmitted from one side bar code and emitted from the other side bar code. The laser beam of the blood sample is converted into a digital signal value, and the second signal value is the laser beam that penetrates the blood sample when the laser beam receiver receives the laser beam that transmits the blood sample from one side barcode and shoots out from the slit 4 The third signal value is a signal value that penetrates the blood sample when the laser beam receiver receives the transmitted blood sample from the slit and emits from the barcode on the other side and converts it into a digital signal value. Signal value:

Figure RE-GDA0003623245350000091
Figure RE-GDA0003623245350000091

本发明涉及的一种试管成分检测方法,具体包括:A test tube component detection method involved in the present invention specifically includes:

将装载有目标样本的样本试管2放置于所述旋转装置1中;Place the sample test tube 2 loaded with the target sample in the rotating device 1;

所述旋转装置驱动对样本试管进行360°旋转;The rotating device drives the sample test tube to rotate 360°;

所述激光束发射器发射透射血液样本的激光束;the laser beam transmitter emits a laser beam that transmits the blood sample;

所述激光束接收器接收穿透所述血液样本的激光束,并根据接收的激光生成第一信号值、第二信号值和第三信号值;the laser beam receiver receives a laser beam penetrating the blood sample, and generates a first signal value, a second signal value and a third signal value according to the received laser light;

所述激光束接收器将所生成的第一信号值、第二信号值和第三信号值反馈至处理器;the laser beam receiver feeds back the generated first signal value, second signal value and third signal value to the processor;

所述处理器将所接收到的目标样本的第一信号值、第二信号值和第三信号值与所述信号阈值组的各血液质量分级下的信号阈值范围条件进行匹配,所述处理器根据匹配结果分析目标样本的质量情况,即目标样本的第一信号值、第二信号值和第三信号值分别同时满足第一正常信号阈值N1、第二正常信号阈值N2、第三正常信号阈值N3的信号值范围时才判定为正常血液样本,目标样本的第一信号值、第二信号值和第三信号值分别同时满足第一轻度信号阈值M1、第二轻度信号阈值M2、第三轻度信号阈值M3的信号值范围时才判定为轻度脂血的血液样本,目标样本的第一信号值、第二信号值和第三信号值分别同时满足第一重度信号阈值H1、第二重度信号阈值H2、第三重度信号阈值H3的信号值范围时才判定为重度脂血的血液样本。The processor matches the received first signal value, second signal value and third signal value of the target sample with the signal threshold range conditions under each blood quality classification of the signal threshold group, the processor Analyze the quality of the target sample according to the matching result, that is, the first signal value, the second signal value and the third signal value of the target sample satisfy the first normal signal threshold N 1 , the second normal signal threshold N 2 , the third normal signal threshold N 2 , and the third normal signal value simultaneously. A normal blood sample is determined only when the signal value range of the signal threshold N3 is within the range of the signal value of the signal threshold N3, and the first signal value, the second signal value and the third signal value of the target sample satisfy the first light signal threshold M 1 and the second light signal respectively. A blood sample with mild lipemia is determined only when the signal value ranges of the threshold M 2 and the third mild signal threshold M 3 are within the range of the threshold value M 2 and the third mild signal threshold value M 3 , and the first signal value, the second signal value and the third signal value of the target sample satisfy the first signal value, the second signal value and the third signal value respectively. A blood sample with severe lipidemia is determined only when the signal value ranges of the severe signal threshold H 1 , the second severe signal threshold H 2 , and the third severe signal threshold H 3 are.

本发明所述的试管成分检测装置及方法,通过旋转装置对样本试管进行旋转,使激光束发射器能够从多个位置状态穿透血液样本,激光束接收器对不同位置状态接收到的激光束转换成不同的数字信号值,包括所述激光束接收器接收从一侧条码发射透射血液样本并从另一侧条码射出时的穿透所述血液样本的激光束并转换成数字信号的第一信号值,所述激光束接收器接收从一侧条码发射透射血液样本并从缝隙4射出时的穿透所述血液样本的激光束并转换成数字信号的第二信号值,所述激光束接收器接收从缝隙发射透射血液样本并从另一侧条码射出时的穿透所述血液样本的激光束并转换成数字信号的第三信号值,并将第一信号值、第二信号值和第三信号值反馈至处理器,处理器将第一信号值、第二信号值和第三信号值与第一正常信号阈值N1、第二正常信号阈值 N2、第三正常信号阈值N3、第一轻度信号阈值M1、第二轻度信号阈值M2、第三轻度信号阈值M3、第一重度信号阈值H1、第二重度信号阈值H2、第三重度信号阈值H3的信号值范围进行匹配判断,只有当第一信号值、第二信号值和第三信号值分别均落入某一级别的各三个信号阈值范围时才判定血液质量结果,该方案,一方面,解决了现有技术中由于样本试管所贴条码的粘贴位置可能存在一定的个体或批量差异导致的影响血液质量的识别准确度的问题,因为该方案中对目标样本信号值得获取有三个位置状态,充分考虑了条码的粘贴情况,不受条码粘贴的影响,另一方面,通过对三个位置状态获取的三个信号值进行综合判定,需同时满足对应级别血液质量的三个信号阈值范围条件方才判断目标样本落入相应级别的血液质量范畴,因此,该方法对血液质量的识别准确度高,错误率低。In the test tube component detection device and method of the present invention, the sample test tube is rotated by the rotating device, so that the laser beam transmitter can penetrate the blood sample from multiple positions, and the laser beam receiver can detect the laser beam received in different positions. Converting into different digital signal values, including the laser beam receiver receiving a first laser beam penetrating the blood sample and converting into a digital signal when the blood sample is transmitted from the barcode on one side and emitted from the barcode on the other side Signal value, the laser beam receiver receives the second signal value of the laser beam that penetrates the blood sample when it is emitted from one side of the barcode and transmits the blood sample and exits from the slit 4 and is converted into a digital signal, the laser beam receives The receiver receives the laser beam that penetrates the blood sample when it transmits the blood sample from the slit and exits from the barcode on the other side and converts it into a third signal value of a digital signal, and converts the first signal value, the second signal value and the third signal value. The three signal values are fed back to the processor, and the processor compares the first signal value, the second signal value and the third signal value with the first normal signal threshold N1, the second normal signal threshold N2, the third normal signal threshold N3, the first normal signal threshold N1, the first normal signal threshold The signal value ranges of the severe signal threshold M1, the second mild signal threshold M2, the third mild signal threshold M3, the first severe signal threshold H1, the second severe signal threshold H2, and the third severe signal threshold H3 are matched and judged, The blood quality result is determined only when the first signal value, the second signal value and the third signal value all fall within each of the three signal threshold ranges of a certain level. This solution, on the one hand, solves the problem in the prior art because There may be some individual or batch differences in the sticking position of the barcode attached to the sample test tube, which may affect the recognition accuracy of blood quality, because in this scheme, there are three position states for the acquisition of the target sample signal, which fully considers the sticking of the barcode. , which is not affected by barcode pasting. On the other hand, by comprehensively judging the three signal values obtained from the three position states, it is necessary to meet the three signal threshold range conditions of the corresponding level of blood quality at the same time to determine that the target sample falls into the corresponding level. Therefore, the method has high recognition accuracy and low error rate for blood quality.

申请人声明,以上所述实施例仅表达了本发明的基本原理、主要特征和优点。本行业的技术人员应该了解,本发明不受上述实施例的限制,上述实施例和说明书中描述的只是本发明的原理,对于本行业的普通技术人员来说,在不脱离本发明构思和范围的前提下,还可以做出各种变化和改进,这些变化和改进都落入要求保护的本发明范围内。The applicant declares that the above-described embodiments merely express the basic principles, main features and advantages of the present invention. Those skilled in the art should understand that the present invention is not limited by the above-mentioned embodiments, and the above-mentioned embodiments and descriptions describe only the principles of the present invention. On the premise of the invention, various changes and improvements can also be made, and these changes and improvements all fall within the scope of the claimed invention.

本发明并不限于上述实施方式,凡采用与本发明相似结构及其方法来实现本发明目的的所有实施方式均在本发明保护范围之内。The present invention is not limited to the above-mentioned embodiments, and all embodiments adopting structures and methods similar to those of the present invention to achieve the purpose of the present invention are within the protection scope of the present invention.

Claims (10)

1. A test tube component detection device is characterized by comprising a rotating device, a laser beam transmitter, a laser beam receiver and a processor, wherein the laser beam transmitter is arranged opposite to the laser beam receiver, and the laser beam receiver comprises a photodiode and a signal amplifier;
the rotating device is used for bearing the sample test tube and rotating the sample test tube;
the laser beam emitter is used for emitting a laser beam which is transmitted through the blood sample;
the laser beam receiver is used for receiving the laser beam penetrating the blood sample and generating a signal value according to the received laser;
the processor is used for analyzing and judging the blood quality grading according to the signal value.
2. A cuvette composition detection apparatus according to claim 1, wherein the rotation means is provided between the laser beam emitter and the laser beam receiver for rotating the loaded sample cuvette between a laser emitting area of the laser beam emitter and a laser receiving area of the laser beam receiver.
3. A cuvette composition detection apparatus according to claim 1, wherein the processor comprises a database system for storing sets of signal thresholds for different quality grades of blood and an analysis system; the analysis system is used for analyzing blood quality grading.
4. A cuvette composition detection apparatus according to claim 3, wherein the database system construction method comprises:
setting the grading number and the grading type of the blood quality;
according to the blood quality grading situation, N samples with known blood quality grading are selected in advance, and signal value groups of the N samples with known blood quality grading are obtained, wherein N is a natural number not less than the blood quality grading quantity, and the N samples with known blood quality grading cover each set blood quality grading;
and setting signal threshold values under different blood quality grading states according to the acquired signal value sets of the N samples with known blood quality grading.
5. The test tube component detecting apparatus according to claim 4, wherein a bar code is attached to the sample test tube, a gap is left between the bar code and the sample test tube, the signal value group includes a first signal value, a second signal value, and a third signal value, and the method for acquiring the signal value group of the N samples with known blood quality classifications includes:
the rotating device drives the sample test tube adhered with the bar code to rotate;
acquiring a first signal value when a laser beam is emitted from a bar code on one side and penetrates a blood sample and is emitted from a bar code on the other side;
acquiring a second signal value when the laser beam is emitted from the bar code on one side and penetrates through the blood sample and is emitted from the gap;
and acquiring a third signal value when the laser beam is emitted from the slit to transmit the blood sample and is emitted from the bar code on the other side.
6. A cuvette composition detection apparatus according to claim 5, wherein the first signal value is smaller than the second signal value, and the second signal value is smaller than the third signal value.
7. The test tube component detection device according to claim 6, wherein the blood quality classification includes normal, mild quality abnormality and severe quality abnormality, the signal threshold set includes a first normal signal threshold, a second normal signal threshold, a third normal signal threshold, a first mild signal threshold, a second mild signal threshold, a third mild signal threshold, a first severe signal threshold, a second severe signal threshold and a third severe signal threshold, and the method for acquiring the signal threshold set includes:
set the known number of samples of normal blood to n1The known number of samples of mild qualitative abnormality is set to n2Setting the known number of samples with severe quality abnormality as n3Wherein n is1+n2+n3N is equal to N, and N1、n2And n3All are natural numbers not less than 1;
respectively acquiring and calculating n according to the method1The mean value of the first signal value, the second signal value and the third signal value of a known sample of normal blood is set to n1Setting n as the first normal signal threshold value as the average value of the first signal values of the known samples of normal blood1The average value of the second signal values of the known samples of the normal blood is a second normal signal threshold value, and n is set1The average of the third signal values of the known samples of normal blood is the third normal signal threshold;
respectively obtaining and calculating n according to the method2Average value of the first signal value, the second signal value and the third signal value of the known sample with mild quality abnormality, and n is set2The average value of the first signal values of the known samples with the slight quality abnormality is a first slight signal threshold value, and n is set2The average value of the second signal values of the known samples with the slight quality abnormality is a second lightness signal threshold value, and n is set2Of mild qualitative abnormalityThe average of the third signal values of the known samples is the third mild signal threshold;
respectively obtaining and calculating n according to the method3Setting n as the average value of the first signal value, the second signal value and the third signal value of the known samples with serious quality abnormality3The average value of the first signal values of the known samples with the serious quality abnormity is a first serious signal threshold value, and n is set3The average value of the second signal values of the known samples with the serious quality abnormity is a second gravity signal threshold value, and n is set3The average of the third signal values of the known samples of severe quality anomalies is the third chrominance signal threshold.
8. The cuvette composition detection apparatus according to claim 7, wherein the first normal signal threshold, the second normal signal threshold, the third normal signal threshold, the first light signal threshold, the second light signal threshold, the third light signal threshold, the first heavy signal threshold, the second heavy signal threshold, and the third heavy signal threshold are set to be N, respectively1、N2、N3、M1、M2、M3、H1、H2And H3Wherein, N is more than or equal to 6001≤700,1500≤N2≤1800,2500≤N3≤6000,500≤M1≤650,800≤M2≤1300,1200≤M3≤5500,500≤H1≤600,650≤H2≤750,700≤H3≤1200。
9. The cuvette composition testing device according to claim 8, wherein the method of analyzing the blood quality grading by the analyzing system comprises:
setting the first signal value, the second signal value and the third signal value of the target sample to be P1, P2 and P3 respectively;
when 600 is less than or equal to P1Not more than 700 and not more than 15002P is not less than 1800 and not more than 25003When the sample is not more than 6000, the target sample is a normal sample;
when P is more than or equal to 5001650 or less and 800 or lessP2Not more than 1300 percent and not more than 1200 and not more than P35500 or less, the target sample is a sample with slight quality abnormality;
when P is more than or equal to 5001Not more than 600, and not more than 650 and not more than P2Not more than 750, and not less than 700P31200 or less, the target sample is a sample with severe quality abnormality.
10. A test tube component detection method using the test tube component detection apparatus according to claim 9, comprising:
placing a sample tube loaded with a target sample in the rotating device;
the rotating device drives the sample test tube to rotate;
the laser beam emitter emits a laser beam that transmits the blood sample;
the laser beam receiver receives a laser beam penetrating the blood sample and generates a first signal value, a second signal value and a third signal value according to the received laser;
the laser beam receiver feeding back the generated first, second and third signal values to a processor;
the processor matching the received first, second and third signal values of the target sample to signal threshold range conditions at each blood quality classification of the set of signal thresholds;
and the processor analyzes the quality condition of the target sample according to the matching result.
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