CN114574381B - Antagonistic strain JYC 314 and its application - Google Patents

Abstract

The invention relates to antagonistic strains JYC 314 and applications thereof, belonging to the technical field of plant disease biological control. The antagonistic strains JYC 314 provided by the invention can significantly inhibit the growth of various Streptomyces scab and Phytophthora infestans, and can effectively prevent potato scab and It can provide new biocontrol resources for the prevention and control of potato scab and late blight.

Description

Antagonistic strain JYC 314 and its application

technical field

The invention belongs to the technical field of plant disease biological control, specifically, the invention belongs to the technical field of plant disease biological control, and specifically relates to an antagonistic strain JYC 314 that inhibits the growth of Streptomyces scabtus and Phytophthora infestans.

Background technique

Potato scab is a typical soil-borne disease caused by the infection of Streptomyces.spp. Streptomyces only infect the potato epidermis, not deep into the potato flesh. The decline in appearance quality has seriously affected the development of the potato industry, especially the seed potato industry. At present, scholars at home and abroad have reported many methods for the prevention and treatment of potato scab, mainly focusing on the selection of disease-resistant varieties, water control, soil pH adjustment, crop rotation and application of green manure, chemical control and biological control. According to the literature, 26 species of potato scab pathogens have been identified so far, including S.scabies, S.acidiscabies, and S.turigidiscabies. According to relevant data reports, the antagonistic strains that have a certain inhibitory effect on the pathogenic bacteria of potato scab mainly include: Bacillus simplex, Bacillus velesi, Bacillus carinii, Bacillus amyloliquefaciens, Paecilomyces bollworm, Trichoderma aculeatus , Candida citri, etc., but not all antagonistic strains have inhibitory effect on all Streptomyces scabies. For potato scab bio-control resources, bio-control resources are relatively scarce, mainly Bacillus, single bacteriostatic spectrum, no obvious bacteriostatic effect, in addition, there are many kinds of scab pathogens and their composition is complex, which makes the prevention and treatment of potato scab more difficult. Here comes the challenge. Therefore, it is necessary to screen more biocontrol bacteria with good inhibitory effect on various Streptomyces scabies.

Potato late blight is a devastating disease caused by Oomycetes Phytophthora infestans (Mont.) deBary. Late blight can infect the whole potato plant, and leaves, petioles, stems and tubers can be harmed. At present, chemical control still occupies an important position in the control measures of potato late blight at home and abroad. During the epidemic period of late blight, chemical control is the main measure to control the epidemic of potato late blight at present. The spraying of metalaxyl, dimethomorph, downylofenac, etc. combined with the use of early warning system is used to guide potato late blight. The prevention and control of the disease can have a good effect of disease prevention and yield increase. Although chemical control is an effective means of preventing potato diseases and one of the most widely used measures, the frequent use of chemicals for a long time can easily pollute the environment, resulting in excessive pesticide residues in agricultural products; at the same time, it will also cause pathogens to develop resistance or even resistance to chemicals. Medicinal properties, so that the control effect of late blight is reduced, or even completely ineffective.

The use of biological control of plant diseases is to use the antagonism of beneficial microorganisms originally existing in nature to target pathogenic microorganisms to control the harm of pathogenic microorganisms. It has less environmental risk and is an environmentally friendly control technology. With people's attention to food safety and ecological safety, biological control has become one of the important strategies to realize the sustainable development of modern agriculture, and the use of beneficial microorganisms to control plant diseases has broad prospects.

SUMMARY OF THE INVENTION

The invention aims to provide an antagonistic strain that can significantly inhibit the growth of various Streptomyces scab and Phytophthora infestans, and can effectively prevent and treat potato scab and late blight.

To achieve the above object, the present invention is achieved through the following technical solutions:

The invention provides an antagonistic strain JYC 314 (Pseudomonas aeruginosa Pseudomonasaeruginosa), which is currently preserved in the China General Microbiological Culture Collection Center (CGMCC), and the address is Beichen West Road, Chaoyang District, Beijing. No. 1, No. 3, Institute of Microbiology, Chinese Academy of Sciences, the deposit number is CGMCC No. 24047, and the deposit date is December 06, 2021.

The invention provides the application of the antagonistic strain JYC 314 in preventing and treating plant diseases; the plant diseases are late blight or scab of potato.

The invention provides the application of the antagonistic strain JYC 314 in the preparation of plant disease or pathogenic bacteria inhibitor; the pathogenic bacteria is Streptomyces scab or Phytophthora infestans; the plant disease is late blight or scab of potato.

The present invention also provides a pathogen inhibitor, comprising the antagonistic strain JYC314 and/or the bacterial liquid of the antagonistic strain JYC314.

Beneficial effects of the present invention:

In the invention, the antagonistic strains are sampled and isolated from the rhizosphere soil of healthy plants in the fields with severe potato scab disease, and the antagonistic strains are screened and verified by the flat plate confrontation method and the paper disc method. The obtained antagonistic strains were identified from the perspective of this study, aiming to provide new biocontrol resources for the prevention and treatment of potato scab and late blight. The antagonistic strain JYC 314 can significantly inhibit the growth of a variety of Streptomyces scab and Phytophthora infestans, and can effectively prevent and treat potato scab and late blight.

Description of drawings

Fig. 1 is the inhibitory effect of bacterial strain JYC 314 on Streptomyces scabtus-plate confrontation test;

Fig. 2 is the inhibitory effect of bacterial strain JYC 314 on potato late blight-plate confrontation test;

Figure 3 is a phylogenetic tree constructed by strain JYC 314 based on the 16SrDNA sequence;

Fig. 4 is the inhibitory effect of bacterial strain JYC 314 bacterial suspension on a variety of potato scab Streptomyces-disc method;

Fig. 5 is the inhibitory effect of bacterial strain JYC 314 on the growth of various Phytophthora infestans—mycelium weight method;

Figure 6 is the morphological identification of strain JYC 314;

Figure 7 is the physiological and biochemical assay of strain JYC 314 - glycolysis test; a: control; b: antagonistic strain JYC 314;

Figure 8 is the physiological and biochemical assay of strain JYC 314-hydrolysis test of macromolecular substances; a: control; b: antagonistic strain JYC 314;

Figure 9 is a physiological and biochemical assay of strain JYC 314-IMViC test; a: control; b: antagonistic strain JYC 314.

Detailed ways

In order to make the objectives, technical solutions and beneficial effects of the present invention clearer, the preferred embodiments of the present invention will be described in detail below to facilitate the understanding of the skilled person.

Example 1: Isolation and purification of potato scab antagonistic strain JYC 314

1. Soil sample collection

The rhizosphere soil of healthy plants was collected from the field of potato scab disease in Yunnan, the collection depth was 10-20cm, sealed with a sterile ziplock bag, brought back to the laboratory, and stored in a refrigerator at 4°C for the isolation of antagonistic strains .

2. Test target pathogenic microorganisms

3 strains of Streptomyces scabies (S.scabies, S.acidiscabies, S.turigidiscabies) and 3 strains of P.infestans, the strain number and race type are SNK-Q9 (1.2.3.4.5.6 .7.8.9.10.11), DQ-1 (1.2.3.4.5.10), ML-S13-418 (1.2.4.5.6.10), the above strains were all provided by the Potato Disease Laboratory, School of Plant Protection, Yunnan Agricultural University.

3. Test medium

The culture of Streptomyces scabies was carried out using Gao's No. 1 medium or oat agar medium, and the bacterial culture was carried out using beef extract peptone medium. Phytophthora infestans was cultured in rye tomato medium. The specific medium formula is as follows:

Oat agar medium (OMA): 20g oat flakes, 20g agar strips, 1000ml water, pH7.2-7.4;

Beef extract peptone medium (NA): 3g beef extract, 10g peptone, 1000ml water, 20g agar strip, pH7.4-7.6;

Rye tomato medium: 70g rye, 150m tomato juice, 850ml water, 1.2g calcium carbonate, 17g agar strips, natural pH;

4. Isolation and purification of antagonistic strain JYC 314

The strains were isolated by heat-treated soil dilution and isolation method, single colony strains were picked and streaked, purified 3 times in a row, mixed with 70% glycerol at 1:1, and stored in a -80°C refrigerator for later use.

5. Preparation of OMA medium: after weighing each substance according to the above-mentioned medium formula, put the agar strips and oat flakes into 400ml of water and soak them for 30min, use a juicer to break the oat flakes and heat and boil them together with the agar strips. After the medium is cooled, the pH of the medium is adjusted to 7.2-7.4 by 1% sodium hydroxide solution, and then packed into two 1000ml conical flasks, each 500ml, sealed and placed in an autoclave for sterilization at 121°C for 30min , when the air pressure drops to 0Mpa, sterilize it again, pour the medium into a 90mm culture plate, and cool it on the ultra-clean workbench to dry it for later use.

6. Preparation of Streptomyces bacteria suspension: Draw the Streptomyces strain bacteria suspension preserved in the laboratory on the surface of the OMA medium plate, spread it evenly with a sterile metal applicator, seal it with a parafilm and place it upside down in a microbial incubator. Incubate in the dark at 28°C for 7-10 days. After the surface of the medium is covered with Streptomyces scabies, add 2ml of sterile water to the petri dish on the ultra-clean workbench, and use a sterile spatula to scrape the Streptomyces from the surface of the medium , the pipette gun sucks the liquid in the dish and flushes the surface of the medium for 2-3 times, and transfers the liquid in the dish to a 5ml sterile centrifuge tube for later use.

7. Screening of antagonistic strains

The antagonistic effect of strain JYC 314 on Streptomyces scabbies was determined by plate confrontation culture method. The specific operation method is as follows: inoculate the purified strain JYC 314 on an oat agar medium plate coated with Streptomyces scabies bacterial suspension, seal it with a parafilm and place it in a 28°C incubator for 7 days of dark cultivation, so as to inoculate only Streptomyces scabies The OMA plate of the bacterial suspension is the control. When the culture time expires, the strains with better bacteriostatic effect are screened according to whether the medium plate has a bacteriostatic zone and the size of the bacteriostatic zone; The three species of Streptomyces scabies could grow normally in oat agar medium and covered the entire surface of the medium without a zone of inhibition; while the surface of the medium inoculated with the JYC 314 strain all showed a zone of inhibition, indicating that the strain JYC 314 All can inhibit the growth of S. scabies S. turigidiscabies, S. acidiscabies, S. scabies, and have inhibitory effects on three kinds of S. scabies (Figure 1).

The antagonism of strain JYC 314 against Phytophthora infestans was determined by the plate confrontation culture method, and the rye tomato medium plate filled with Phytophthora infestans mycelium and the separate rye tomato medium were respectively grown with a sterile puncher. Punch holes on the plate, invert the fungus cake containing Phytophthora infestans into the small hole in the center of the rye medium plate, and pick the bacteria of the strain to be tested to the rye tomato containing Phytophthora infestans fungus cake. On the medium plate, it was placed in a microbial incubator at 20°C for dark cultivation for 7-10 days. At the end of the cultivation, the growth diameter of the colony was measured by the cross-cross measurement method. The results of the plate confrontation culture showed that the Phytophthora infestans in the control medium could grow normally and cover the entire rye tomato medium plate, and the colony growth diameter was 56.19 mm. The growth diameters of the colonies were 14.27 mm and 0 mm, respectively, which were significantly different from those of the control (P<0.05), indicating that the strain JYC 314 had a significant inhibitory effect on the growth of Phytophthora infestans (Table 1, Figure 2).

Table 1 Inhibitory effect of strain JYC 314 on P. infestans

Note: The lowercase letters in the table indicate the significance of the difference at the 0.05 level

In summary, according to the results of the confrontation culture on the plate, a strain with inhibitory effect on the growth of Streptomyces scabbies and Phytophthora infestans was obtained, which was named JYC 314.

Example 2: Molecular biological identification of antagonistic strain JYC 314

1. Extraction of bacterial genomic DNA

The single colony of the strain JYC314 prepared in Example 1 and purified and cultivated was inoculated on the beef extract peptone culture plate, and after culturing in the dark at 28°C for 120 hours, the bacterial genome DNA extraction kit of Shanghai Sangon EZUP column was used to extract the strain of JYC314. Genomic DNA, the specific steps are as follows:

(1) Take 0.5ml-1ml of overnight cultured bacterial liquid, add it to a 1.5ml centrifuge tube, centrifuge at 8000rpm at room temperature for 1min, discard the supernatant, and remove the bacteria. Add 100 microliters of BufferDigestion and 80 microliters of 20 mg/ml lysozyme solution, and water bath at 37°C for 30 min. After the water bath is completed, add 20 microliters of Proteinase K solution, and shake to mix well. In a water bath at 56 °C for 30 min, the cells were completely lysed. After the water bath, add 20 microliters of 10 mg/ml RNaseA solution, leave it at room temperature for 2-5min,

(2) Add 200 microliters of BufferBD, fully invert and mix, and water bath at 70°C for 10 minutes.

(3) Add 200 microliters of absolute ethanol, fully invert and mix.

(4) Put the adsorption column into the collection tube, add all the solution and translucent fibrous suspension into the adsorption column with a pipette, let stand for 2 min, centrifuge at 12000 rpm for 1 min at room temperature, and pour out the waste liquid in the collection tube.

(5) put the adsorption column into the collection tube, add 500 microliters of PW Solution, centrifuge at 10000rpm for 30s and pour off the filtrate;

(6) put the adsorption column into the collection tube, add 500 microliters of Wash Solution, and centrifuge at 10000rpm for 30s to pour off the filtrate;

(7) put the adsorption column into the collection tube, add 500 microliters of PW Solution, centrifuge at 12000rpm for 2min and pour off the filtrate;

(8) Take out the adsorption column, put it into a new 1.5 ml centrifuge tube, add 50-100 microliters of CE Buffer, let it stand for 3 minutes, centrifuge at 12000 rpm for 2 minutes, and collect the DNA solution. The extracted DNA was stored at -20°C.

2. Amplification of 16S rDNA sequences

The 16S rDNA of strain JYC 314 was amplified using the universal primers 27F (5'-AGTTTGATCMTGGCTCAG-3') and 1492R (5'-GGTTACCTTGTTACGACTT-3') using the genomic DNA of strain JYC 314 as a template. PCR amplification conditions were 30 cycles of pre-denaturation at 94°C/4min, denaturation at 94°C/45s, annealing at 55°C/45s, extension at 72°C/1min, and final extension at 72°C/10min. The PCR reaction system is as follows:

Table 2 PCR reaction system

The PCR amplification product was detected by agarose gel electrophoresis. The results showed that a bright band was obtained, and the amplification product of about 1500bp was obtained. After the electrophoresis band was recovered, the above-mentioned primers were used to sequence the above-mentioned amplified gene fragments. The work was completed by Shanghai Sangon Bioengineering Co., Ltd. The nucleotide sequence length of the 16SrDNA of the strain was measured to be 1429bp, and the sequencing results of the 16SrDNA gene were as follows:

ATGGCTCAGATTGAACGCTGGCGGCAGGCCTAACACATGCAAGTCGAGCGGATGAAGGGAGCTTGCTCCTGGATTCAGCGGCGGACG GGTGAGTAATGCCTAGGAATCTGCCTGGTAGTGGGGGATAACGTCCGGAAACGGGCGCTAATACCGCATACGTCCTGAGGGAGAAAGTGGG GGATCTTCGGACCTCACGCTATCAGATGAGCCTAGGTCGGATTAGCTAGTTGGTGGGGTAAAGGCCTACCAAGGCGACGATCCGTAACTGG TCTGAGAGGATGATCAGTCACACTGGAACTGAGACACGGTCCAGACTCCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGGCGAAAG CCTGATCCAGCCATGCCGCGTGTGTGAAGAAGGTCTTCGGATTGTAAAGCACTTTAAGTTGGGAGGAAGGGCAGTAAGTTAATACCTTGCT GTTTTGACGTTACCAACAGAATAAGCACCGGCTAACTTCGTGCCAGCAGCCGCGGTAATACGAAGGGTGCAAGCGTTAATCGGAATTACTG GGCGTAAAGCGCGCGTAGGTGGTTCAGCAAGTTGGATGTGAAATCCCCGGGCTCAACCTGGGAACTGCATCCAAAACTACTGAGCTAGAGT ACGGTAGAGGGTGGTGGAATTTCCTGTGTAGCGGTGAAATGCGTAGATATAGGAAGGAACACCAGTGGCGAAGGCGACCACCTGGACTGAT ACTGACACTGAGGTGCGAAAGCGTGGGGAGCAAACAGGATTAGATACCCTGGTAGTCCACGCCGTAAACGATGTCGACTAGCCGTTGGGAT CCTTGAGATCTTAGTGGCGCAGCTAACGCGATAAGTCGACCGCCTGGGGAGTACGGCCGCAAGGTTAAAACTCAAATGAATTGACGGGGGC CCGCACAAGCGGTGGAGCATGTGGTTTAATTCGAAGCAACGCGAAGAACCTTACCTGGCCTTGACATGCTGAGAACTTTCCAGA GATGGAT TGGTGCCTTCGGGAACTCAGACACAGGTGCTGCATGGCTGTCGTCAGCTCGTGTCGTGAGATGTTGGGTTAAGTCCCGTAACGAGCGCAAC CCTTGTCCTTAGTTACCAGCACCTCGGGTGGGCACTCTAAGGAGACTGCCGGTGACAAACCGGAGGAAGGTGGGGATGACGTCAAGTCATC ATGGCCCTTACGGCCAGGGCTACACACGTGCTACAATGGTCGGTACAAAGGGTTGCCAAGCCGCGAGGTGGAGCTAATCCCATAAAACCGATCGTAGTCCGGATCGCAGTCTGCAACTCGACTGCGTGAAGTCGGAATCGCTAGTAATCGTGAATCAGAATGTCACGGTGAATACGTTCCCG GGCCTTGTACACACCGCCCGTCACACCATGGGAGTGGGTTGCTCCAGAAGTAGCTAGTCTAACCGCAA

The sequencing results were entered into the National Center for Biotechnology Information (NCBI) database, and the BLAST software was used to compare the homology with other 16S rDNA sequences in GenBank. The 16S rDNA sequences of these strains were completely matched. Select the sequence that is similar to that of strain JYC 314, and use the phylogenetic analysis software MEGA7.0 to construct a phylogenetic tree by Neighbor-Joining Method (Figure 3). Pseudomonas aeruginosa strain S-04 MT626658.1) is in the same phylogeny, with the closest evolutionary relationship and 99.31% homology. It is preliminarily judged that the strain JYC 314 is Pseudomonas aeruginosa (Pseudomonas aeruginosa).

Example 3: Effect of antagonistic strain JYC 314 bacterial suspension on the growth of Streptomyces scabtus

1. Materials and methods

Test material:

Test strains: S.scabies, S.acidiscabies, S.turigidiscabies;

Other materials: 18mm qualitative round filter paper, metal coating rod, 90mm petri dish, parafilm, pipette gun, centrifuge tube, sterile water, juicer, metal spatula, metal tweezers, digital vernier caliper;

experiment method:

The preparation methods of OMA medium and Streptomyces suspension are the same as in Example 1.

Preparation of the antagonistic strain bacterial suspension: Pick the purified antagonistic strain with a sterile pipette tip and put it in a 5ml sterile centrifuge tube containing 1ml sterile water, and use a shaker to vortex for 30S before use.

The Streptomyces scabii inhibition test was carried out by the paper disc method: suck 100 μl of Streptomyces scabies suspension on the OMA medium plate, spread it evenly with a sterile metal coating rod, and place sterile tweezers on the sterile filter paper and place it on the culture medium. In the center of the plate, gently press the filter paper along the periphery to make it close to the surface of the medium. Pipette aspirate 50 μl of the bacterial suspension of the strain to be tested and slowly release it on the filter paper. It was placed in a microbial incubator, and cultivated in the dark at 28°C for 7-10 days, inoculated with sterile water on the OMA medium plate coated with Streptomyces scabies bacterial suspension and placed a filter paper sheet as a control. The diameter of the zone of inhibition was determined by the cross-measurement method.

2. Results and Analysis

The inhibition zone method was used to test the inhibitory activity of strain JYC 314 against three S. turigidiscabies, S. acidiscabies and S. scabies. The results showed that the test results showed that both control 1 and control 2 could cover the surface of the medium, and there was no inhibition zone. A bacteriostatic zone appeared on the surface of the medium to which the strain JYC 314 to be tested was added, and the diameters of the bacteriostatic zone were 27.75 mm, 27.50 mm, and 38.66 mm, and were significantly different from the control (0 mm) (p< 0.05), indicating that strain JYC 314 can significantly inhibit the growth of scab pathogens S. scabies, S. turigidiscabies, S. acidiscabies, and has the most obvious inhibitory effect on S. scabies. (Figure 4)

Table 3 Effects of strain JYC 314 on the growth of potato scab pathogens

Note: The lowercase letters in the table indicate the significance of the difference at the 0.05 level

Example 4: Effects of antagonistic strain JYC 314 on the growth of various Phytophthora infestans

1. Materials and methods

Tested Phytophthora infestans strain number and physiological race type: SNK-Q9 (1.2.3.4.5.6.7.8.9.10.11), DQ-1 (1.2.3.4.5.10), ML-S13-418 (1.2. 4.5.6.10).

Other materials: rake bamboo sticks, 5mm hole punch, 90mm petri dish, parafilm, 1/10,000 scale.

experiment method:

The preparation method of rye tomato culture medium is the same as that in Example 1.

The antagonistic effect of strain JYC 314 against Phytophthora infestans was determined by plate confrontation culture. The specific operation method is as follows: use a sterile puncher to punch holes on a rye tomato medium plate covered with Phytophthora infestans mycelium and a separate rye tomato medium plate, and make holes containing Phytophthora infestans mycelium. The bacteria cake was inverted into the small hole in the center of the rye culture medium plate, and the bacteria of the strain to be tested were picked onto the rye tomato culture medium plate containing the Phytophthora infestans cake, and placed in a 20°C microbial incubator. Dark culture for 7-10 days. At the end of the culture, the Phytophthora infestans mycelium in the petri dish was peeled off from the surface of the rye tomato medium by using a rake-type bamboo stick. as a measure of mycelial growth.

2. Results and Analysis

The hyphae weights of ML-S13-418, DQ-1, SNK-Q93 Phytophthora infestans under the stress of strain JYC 314 were determined. The results showed that the three strains in the control culture could grow normally, and the mycelial weights were 0.0169g, 0.0488g, and 0.0618g, respectively. In the medium inoculated with strain JYC 314, ML-S13-418, DQ-1, SNK The mycelial weight of -Q9 was significantly lower than that of the control (p<0.05), indicating that under the stress of strain JYC 314, the mycelial growth was significantly inhibited.

Table 4 Effects of strain JYC314 on mycelial growth of P. infestans

Note: The lowercase letters in the table indicate the significance of the difference at the 0.05 level

Example 5: Morphological identification of antagonistic strain JYC 314

The isolated and purified single colony of JYC 314 strain was streaked and cultured on beef extract peptone medium for 48 hours. The morphological characteristics of the colony were observed, and the cell wall type of the bacteria was determined by Gram staining. The morphological observation results showed that the strain JYC 314 grew well on the beef extract peptone medium, and the colonies were milky white or grayish white, round, smooth and moist, with a raised center and irregular edges. 5.0 μm, Gram positive, spore-forming, aerobic or facultative anaerobic growth. The surrounding of the colony was pale green, and the green color deepened with the extension of culture time, and later turned purple or brown (Fig. 6).

Example 6: Physiological and biochemical assay results of antagonistic strain JYC 314

See "Common Bacterial System Identification Manual" for sugar fermentation, gelatin liquefaction test, hydrogen sulfide production test, starch hydrolysis test, methyl red test, VP test, indole production test, milk litmus test, citric acid test. Determination of physiological and biochemical indicators such as salt utilization test and urease production test. Physiological and biochemical assay results show that strain JYC 314 can decompose glucose to produce acid, produce gelatinase to decompose gelatin, utilize citrate, and produce H ₂ S gas. The result of milk litmus test is positive; strain JYC 314 does not degrade lactose and does not hydrolyze Starch did not produce urease to decompose urea, and the results of methyl red test, indole production test and VP test were all negative (see Table 5, Figures 7-9).

Table 5 Physiological and biochemical characteristics of antagonistic strain JYC314

Note: "+" in the table indicates a positive result; "-" indicates a negative result.

To sum up, according to the SrDNA sequence analysis, morphological characteristics, physiological and biochemical characteristics of the strain JYC 31416, combined with the "Common Bacterial System Identification Manual", it is judged that the strain JYC 314 belongs to Pseudomonas aeruginosa, and this strain is resistant to a variety of potato Streptomyces scab and Phytophthora infestans have significant inhibitory effects, which can effectively prevent potato scab and late blight.

Finally, it should be noted that the above preferred embodiments are only used to illustrate the technical solutions of the present invention and not to limit them. Although the present invention has been described in detail through the above preferred embodiments, those skilled in the art should Various changes may be made in details without departing from the scope of the invention as defined by the claims.

SEQUENCE LISTING

<110> Yunnan Agricultural University

<120> Antagonistic strain JYC314 and its application

<130> 2021

<160> 1

<170> PatentIn version 3.5

<210> 1

<211> 1429

<212> DNA

<213> Artificial sequences

<400> 1

atggctcaga ttgaacgctg gcggcaggcc taacacatgc aagtcgagcg gatgaaggga 60

gcttgctcct ggattcagcg gcggacgggt gagtaatgcc taggaatctg cctggtagtg 120

ggggataacg tccggaaacg ggcgctaata ccgcatacgt cctgagggag aaagtggggg 180

atcttcggac ctcacgctat cagatgagcc taggtcggat tagctagttg gtggggtaaa 240

ggcctaccaa ggcgacgatc cgtaactggt ctgagaggat gatcagtcac actggaactg 300

agacacggtc cagactccta cgggaggcag cagtggggaa tattggacaa tgggcgaaag 360

cctgatccag ccatgccgcg tgtgtgaaga aggtcttcgg attgtaaagc actttaagtt 420

gggaggaagg gcagtaagtt aataccttgc tgttttgacg ttaccaacag aataagcacc 480

ggctaacttc gtgccagcag ccgcggtaat acgaagggtg caagcgttaa tcggaattac 540

tgggcgtaaa gcgcgcgtag gtggttcagc aagttggatg tgaaatcccc gggctcaacc 600

tgggaactgc atccaaaact actgagctag agtacggtag agggtggtgg aatttcctgt 660

gtagcggtga aatgcgtaga tataggaagg aacaccagtg gcgaaggcga ccacctggac 720

tgatactgac actgaggtgc gaaagcgtgg ggagcaaaca ggattagata ccctggtagt 780

ccacgccgta aacgatgtcg actagccgtt gggatccttg agatcttagt ggcgcagcta 840

acgcgataag tcgaccgcct ggggagtacg gccgcaaggt taaaactcaa atgaattgac 900

gggggcccgc acaagcggtg gagcatgtgg tttaattcga agcaacgcga agaaccttac 960

ctggccttga catgctgaga actttccaga gatggattgg tgccttcggg aactcagaca 1020

caggtgctgc atggctgtcg tcagctcgtg tcgtgagatg ttgggttaag tcccgtaacg 1080

agcgcaaccc ttgtccttag ttaccagcac ctcgggtggg cactctaagg agactgccgg 1140

tgacaaaccg gaggaaggtg gggatgacgt caagtcatca tggcccttac ggccagggct 1200

acacacgtgc tacaatggtc ggtacaaagg gttgccaagc cgcgaggtgg agctaatccc 1260

ataaaaccga tcgtagtccg gatcgcagtc tgcaactcga ctgcgtgaag tcggaatcgc 1320

tagtaatcgt gaatcagaat gtcacggtga atacgttccc gggccttgta cacaccgccc 1380

gtcacaccat gggagtgggt tgctccagaa gtagctagtc taaccgcaa 1429

Claims (4)

1. Antagonistic strain Pseudomonas aeruginosa (Pseudomonas aeruginosa) JYC314, characterized by: the preservation number is CGMCC NO. 24047.

2. The antagonistic strain Pseudomonas aeruginosa (Pseudomonas aeruginosa) JYC314 as claimed in claim 1 for use in the prevention and treatment of potato late blight or potato scab.

3. The use of the antagonistic strain Pseudomonas aeruginosa (Pseudomonas aeruginosa) JYC314 as claimed in claim 1 for the preparation of a pathogen inhibitor, wherein: the pathogenic bacteria are Streptomyces scabies or Phytophthora infestans.

4. A pathogen inhibitor characterized by: a bacterial suspension comprising the antagonistic strain Pseudomonas aeruginosa (Pseudomonas aeruginosa) JYC314 or the antagonistic strain Pseudomonas aeruginosa (Pseudomonas aeruginosa) JYC314 of claim 1.

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