CN114573754A - Humic acid type mulberry seed coating agent and preparation method and use method thereof - Google Patents

Abstract

The invention discloses a humic acid type mulberry seed coating agent and a preparation method thereof, wherein a sodium alginate aqueous solution and a potassium humate solution are mixed, a cross-linking agent and an initiator are added, and polymerization reaction is initiated by ultraviolet lamp light irradiation to obtain a viscous liquid, namely the humic acid type mulberry seed coating agent; the humic acid type mulberry seed coating agent disclosed by the invention coats mulberry seeds in an artificial seed coating mode: sterilizing and cleaning mulberry seeds; the pretreated seeds and the humic acid type mulberry seed coating agent are mixed according to the mass ratio (20-40): 1, coating, taking out the seeds after 4 hours, and naturally drying; the humic acid type mulberry seed coating agent adopts an acrylamide/sodium alginate double-network hydrogel structure, has higher water retention performance, is beneficial to seed development and drug slow release, is beneficial to absorption of water and nutrient substances in soil by seeds, improves the germination rate and the fresh weight of the seeds, and has the advantages of simple operation and low input cost.

Description

A kind of humic acid type mulberry seed coating agent and preparation method and use method thereof

technical field

The invention belongs to the technical field of seed coating, and in particular relates to a humic acid type mulberry seed coating agent and a preparation method and use method thereof.

Background technique

Mulberry seeds are small fatty particles, which are prone to deterioration and shorten lifespan in the presence of external unfavorable factors such as high temperature, humidity, mold parasites and insect erosion. Therefore, how to improve the germination rate and vitality of mulberry seeds is a problem that needs to be solved in mulberry planting.

Seed coating is to wrap the seed coating agent with insecticide, sterilization, nutrition supply, growth regulation and other ingredients on the surface of the seeds, so that a smooth and firm film is formed outside the seeds. With the gradual development of the embryo in the seed and the continuous growth of the seedling, the seed coating agent will slowly release various active ingredients contained in the seed coating, which will be gradually absorbed into the body by the seedling, so as to prevent and control diseases and insect pests in the seedling stage, promote growth and development, and improve crop yield. the goal of.

The seed coating technology has the advantages of convenient operation and good sterilization and insecticidal effect. The use of seed coating agents to treat seeds not only saves time and labor, but also conforms to the national policy of reducing chemical fertilizers and pesticides. It also plays an important role in increasing production and income. However, the existing seed coating agents have certain limitations, such as: the components of the coating agent are easy to poison the seeds, the water retention performance is poor, the slow release of the drug is slow, and the cost of the coating agent is high. Therefore, it is necessary to develop a mulberry seed coating agent with high water retention performance, fast drug release, and increased production and income.

SUMMARY OF THE INVENTION

In view of the deficiencies in the prior art, the object of the present invention is to provide a humic acid-type mulberry seed coating agent and its preparation method and use method, which have high water retention performance and fast drug release, and are used to improve the germination rate of mulberry seeds and Vitality, increase production and income.

In order to achieve the above object, the present invention adopts the following technical solutions to realize:

A preparation method of a humic acid type mulberry seed coating agent, in parts by weight, comprising the following steps:

Step 1: Add 1-3 parts of sodium alginate powder to 100-300 parts of deionized water, stir at room temperature for 4-5 hours to fully swell the sodium alginate; then continue to stir in a constant temperature water bath at 50-60°C for 0.5-1 hour , so that the sodium alginate is fully dissolved, and finally a transparent, uniform and stable sodium alginate aqueous solution is prepared for use;

Step 2: Dissolving 1-10 parts of potassium humate in 100-200 parts of deionized water to obtain potassium humate solution;

Step 3, taking 5-10 parts of sodium alginate aqueous solution, keeping the temperature in a constant temperature water bath at 50-60 ° C for 10-15 min, then adding 15-20 parts of potassium humate solution, stirring for 20-25 min to obtain a mixed solution;

Step 4: Pass nitrogen for 10-15 minutes into the mixed solution obtained in step 3, and add 10-14 parts of acrylamide monomer and 6-10 parts of acrylic monomer with a neutralization degree of 70% and continue stirring for 10-15 minutes;

Step 5. After the solution is uniform and stable, continue to pass nitrogen for 10-15 minutes to deoxygenate, add 6-8 parts of PEG-400, 0.1-0.2 parts of glycerol, and 0.4-0.6 parts of cross-linking agent, stir for about 2-5 minutes, and finally add 0.1～0.2 part of initiator, at 50～70℃, pass nitrogen for 30～35min to obtain a mixed system;

Step 6, under the condition of 50-55 ℃ heat preservation, irradiate the mixed system with ultraviolet light to initiate a polymerization reaction, and react for two hours to obtain a viscous liquid, which is the humic acid type mulberry seed coating agent.

The present invention also has the following technical features:

Preferably, the crosslinking agent is MBA, epichlorohydrin or acrylamide.

Preferably, the initiator is potassium persulfate, ammonium persulfate or myrcylamine peroxide.

Preferably, the irradiation wavelength of the ultraviolet lamp is 250-350 nm.

The present invention also protects a humic acid type mulberry seed coating agent prepared by the above method.

The present invention also protects a method for using the above-mentioned humic acid type mulberry seed coating agent, which adopts an artificial seed coating method to coat the mulberry seeds.

Preferably, the following steps are included:

Step 1: Disinfect, wash and pre-treat the mulberry seeds;

Step 2: The pretreated seeds and the humic acid type mulberry seed coating agent are coated according to the mass ratio (20-40): 1, and the seeds are taken out after 4 hours and dried naturally.

Further, the disinfection method is to immerse and disinfect the mulberry seeds in HgCl ₂ solution for 15-20 minutes.

Further, the concentration of the HgCl ₂ solution is 0.08-0.1%.

Preferably, the cleaning method is to rinse with distilled water.

Compared with the prior art, the present invention has the following technical effects:

The humic acid type mulberry seed coating agent of the present invention adopts acrylamide/sodium alginate double network hydrogel structure, the water content in this double network hydrogel exceeds 90%, and its breaking energy is very high , can reach more than 9000J/m ² , and can stretch it more than 20 times its body length, so its mechanical properties are very superior; in addition, its water absorption is moderate, with a more stable swelling rate, so it has higher water retention performance, which is beneficial to seed development and drug release;

When the humic acid type mulberry seed coating agent is prepared by the invention, the natural product potassium humate is used as the basic raw material; the humic acid has acidic groups and basic groups inside, and sodium alginate contains a lot of -COO-, These -COO- will combine with H+ in the acidic solution to generate -COOH. With the gradual increase of the pH of the solution, the ionization degree of -COOH will increase, which will greatly improve the hydrophilicity of sodium alginate. , thereby strengthening the water retention effect of humic acid, which is conducive to the absorption of water and nutrients in the soil by seeds, and improves the germination rate of seeds and the fresh weight of seedlings;

The humic acid type mulberry seed coating agent and using method of the present invention have the advantages of simple operation, low input cost, fast film forming speed, can be mixed with various pesticides and micro-fertilizers, can also improve the vitality of mulberry seeds, promote germination, and is environmentally friendly. Features.

Description of drawings

Fig. 1 is the preparation flow chart of the present invention.

Detailed ways

The specific content of the present invention will be further explained in detail below in conjunction with the embodiments.

Example 1

1) Add 1 part of sodium alginate powder to 100 parts of deionized water, stir at room temperature for 4 hours to fully swell the sodium alginate; then continue to stir in a constant temperature water bath at 50°C for 0.5 hours to fully dissolve the sodium alginate, and the final preparation is made. Obtain a transparent, uniform and stable aqueous solution of sodium alginate for use;

2) 1 part of potassium humate is dissolved in 100 parts of deionized water to obtain potassium humate solution for subsequent use;

3) get 5 parts of sodium alginate aqueous solution, keep warm under 50 ℃ of constant temperature water bath conditions for 10min, then add 15 parts of potassium humate solution, stir to obtain mixed solution after 20min;

4) Pass nitrogen gas into the mixed solution obtained in step 3) for 10 minutes, and add 10 parts of acrylamide monomers and 6 parts of acrylic acid monomers with a neutralization degree of 70% and continue to stir for 10 minutes;

5) After the solution is uniform and stable, continue to pass nitrogen for 10 minutes, after deoxygenation, add 6 parts of PEG-400, 0.1 part of glycerol, 0.4 part of MBA, stir for about 2 minutes, and finally add 0.1 part of potassium persulfate, at 50 ℃, pass through for 30 minutes nitrogen to obtain a mixed system;

6) Irradiate the mixed system in the beaker with light with a wavelength of 250nm ultraviolet lamp to initiate a polymerization reaction, and the whole process is kept at 50°C and reacted for two hours to obtain a viscous liquid, which is a humic acid type mulberry seed coating agent;

7) First, disinfect the mulberry seeds in a HgCl solution with a concentration of 0.08% for ₁₅ min, take out the seeds and rinse them with distilled water. Remove the seeds and let them dry naturally.

Example 2

1) Add 3 parts of sodium alginate powder to 300 parts of deionized water, stir at room temperature for 5 hours to fully swell the sodium alginate; then continue to stir in a constant temperature water bath at 60°C for 1 hour to fully dissolve the sodium alginate, and finally obtain Transparent, uniform and stable sodium alginate aqueous solution, for use;

2) 10 parts of potassium humates are dissolved in 200 parts of deionized water to obtain potassium humates solution, for subsequent use;

3) get 10 parts of sodium alginate aqueous solution, keep warm under 60 ℃ of constant temperature water bath conditions for 15min, add 20 parts of potassium humate solution to the beaker subsequently, obtain mixed solution after stirring for 25min;

4) Pass nitrogen gas into the mixed solution obtained in step 3) for 15 minutes, and add 14 parts of acrylamide monomers and 10 parts of acrylic acid monomers with a neutralization degree of 70% and continue to stir for 15 minutes;

5) After the solution is uniform and stable, continue to pass nitrogen for 15 minutes. After deoxygenation, add 8 parts of PEG-400, 0.2 parts of glycerol, and 0.6 parts of epichlorohydrin, stir for about 5 minutes, and finally add 0.2 parts of ammonium persulfate. , pass 35min nitrogen to obtain a mixed system;

6) Irradiate the mixed system in the beaker with light with a wavelength of 350nm ultraviolet lamp to initiate a polymerization reaction, and the whole process is kept warm and reacted under the condition of 55 ℃ for two hours to obtain a viscous liquid, which is a humic acid type mulberry seed coating agent;

7) First, disinfect the mulberry seeds in a HgCl solution with a concentration of 0.1% for ₂₀ min, take out the seeds and rinse them with distilled water. Remove the seeds and let them dry naturally.

Example 3

1) Add 1 part of sodium alginate powder to 300 parts of deionized water, stir at room temperature for 5 hours to fully swell the sodium alginate; then continue stirring for 1 hour in a 50°C constant temperature water bath to fully dissolve the sodium alginate, and finally obtain Transparent, uniform and stable aqueous solution of sodium alginate, ready for use;

2) 1 part of potassium humate is dissolved in 100 parts of deionized water to obtain potassium humate solution for subsequent use;

3) get 10 parts of sodium alginate aqueous solution, keep warm under 50 ℃ of constant temperature water bath conditions for 15min, then add 20 parts of potassium humate solution to the beaker, and obtain mixed solution after stirring for 20min;

4) Pass 10 minutes of nitrogen into the mixed solution obtained in step 3), and add 14 parts of acrylamide monomers and 6 parts of acrylic acid monomers with a neutralization degree of 70% and continue to stir for 15 minutes;

5) After the solution is uniform and stable, continue to pass nitrogen for 10 minutes, and after deoxygenation, add 8 parts of PEG-400, 0.2 parts of glycerol, 0.4 parts of acrylamide, stir for about 2 minutes, and finally add 0.1 part of myrcenamine peroxide, and at 50 ° C , pass 35min nitrogen to obtain a mixed system;

6) Irradiate the mixed system in the beaker with light with a wavelength of 350nm ultraviolet lamp to initiate a polymerization reaction, and the whole process is kept warm and reacted under the condition of 55 ℃ for two hours to obtain a viscous liquid, which is a humic acid type mulberry seed coating agent;

7) First, disinfect the mulberry seeds in a HgCl solution with a concentration of 0.1% for ₂₀ min, take out the seeds and rinse them with distilled water. Remove the seeds and let them dry naturally.

Example 4

1) Add 3 parts of sodium alginate powder to 200 parts of deionized water, stir at room temperature for 4 hours to fully swell the sodium alginate; Obtain a transparent, uniform and stable aqueous solution of sodium alginate for use;

2) 2 parts of potassium humates are dissolved in 150 parts of deionized water to obtain potassium humate solution, for subsequent use;

3) get 6 parts of sodium alginate aqueous solution, keep warm under 55 ℃ of constant temperature water bath conditions for 15min, then add 18 parts of potassium humate solution to the beaker, and obtain mixed solution after stirring for 20min;

4) Pass nitrogen gas into the mixed solution obtained in step 3) for 10 minutes, and add 10 parts of acrylamide monomers and 8 parts of acrylic acid monomers with a neutralization degree of 70% and continue to stir for 10 minutes;

5) After the solution is uniform and stable, continue to pass nitrogen for 10 minutes, after deoxygenation, add 7 parts of PEG-400, 0.1 part of glycerol, 0.5 part of MBA, stir for about 3 minutes, and finally add 0.1 part of potassium persulfate, at 60 ℃, pass for 30 minutes nitrogen to obtain a mixed system;

6) Irradiate the mixed system in the beaker with light with a wavelength of 300nm ultraviolet lamp to initiate a polymerization reaction, and the whole process is kept at a temperature of 50 ° C and reacted for two hours to obtain a viscous liquid, which is a humic acid type mulberry seed coating agent;

7) First, the mulberry seeds were sterilized in the HgCl solution with a concentration of 0.08% for ₁₅ min, and the seeds were taken out and rinsed with distilled water. Remove the seeds and let them dry naturally.

Example 5

1) Add 2 parts of sodium alginate powder to 150 parts of deionized water, and stir at room temperature for 5 hours to fully swell the sodium alginate; Transparent, uniform and stable sodium alginate aqueous solution, for use;

2) 5 parts of potassium humates are dissolved in 180 parts of deionized water to obtain potassium humates solution, for subsequent use;

3) get 6 parts of sodium alginate aqueous solution, keep warm under 55 ℃ of constant temperature water bath conditions for 10min, then add 18 parts of potassium humate solution to the beaker, and obtain mixed solution after stirring for 25min;

4) Pass nitrogen for 10 minutes into the mixed solution obtained in step 3), and add 12 parts of acrylamide monomers and 8 parts of acrylic acid monomers with a neutralization degree of 70% and continue to stir for 10 minutes;

5) After the solution is uniform and stable, continue to pass nitrogen for 10 minutes, and after deoxygenation, add 7 parts of PEG-400, 0.1 part of glycerol, 0.4 part of epichlorohydrin, stir for about 2 minutes, and finally add 0.1 part of ammonium persulfate, at 50 ° C , pass 30min nitrogen to obtain a mixed system;

6) Irradiate the mixing system in the beaker with light with a wavelength of 280nm ultraviolet lamp to initiate a polymerization reaction, and the whole process is kept at a temperature of 50 ° C and reacts for two hours to obtain a viscous liquid, which is a humic acid type mulberry seed coating agent;

7) First, disinfect the mulberry seeds in a HgCl solution with a concentration of 0.1% for ₁₅ min, take out the seeds and rinse them with distilled water. Remove the seeds and let them dry naturally.

Example 6

1) Add 3 parts of sodium alginate powder to 250 parts of deionized water, and stir at room temperature for 4.5 hours to fully swell the sodium alginate; A transparent, uniform and stable sodium alginate aqueous solution was prepared, which was used for later use;

2) 4 parts of potassium humates are dissolved in 150 parts of deionized water to obtain potassium humates solution, for subsequent use;

3) get 8 parts of sodium alginate aqueous solution, keep warm under 50 ℃ of constant temperature water bath conditions for 12min, then add 15 parts of potassium humate solution to the beaker, and obtain mixed solution after stirring for 22min;

4) Pass nitrogen gas into the mixed solution obtained in step 3) for 13 minutes, and add 10 parts of acrylamide monomers and 8 parts of acrylic monomers with a neutralization degree of 70% and continue stirring for 12 minutes;

5) After the solution is uniform and stable, continue to pass nitrogen for 13 minutes. After deoxygenation, add 7 parts of PEG-400, 0.15 parts of glycerol, and 0.5 parts of acrylamide, and stir for about 2 minutes. Finally, add 0.15 parts of myrcenamine peroxide, at 60 ° C. , pass 32min nitrogen to obtain a mixed system;

6) Irradiate the mixing system in the beaker with light with a wavelength of 300nm ultraviolet lamp to initiate a polymerization reaction, and the whole process is kept at a temperature of 52°C for two hours to obtain a viscous liquid, which is a humic acid type mulberry seed coating agent;

7) First, the mulberry seeds _were disinfected in a HgCl solution with a concentration of 0.09% for 18 min, and the seeds were taken out and rinsed with distilled water. Remove the seeds and let them dry naturally.

式中GI表示发芽指数、Gt表示逐日发芽数、Dt表示相应发芽天数；活力指数＝平均根长×发芽指数。使用量按本专利实施例1说明使用，未经过处理的桑树种子按常规进行施肥培育，具体实验结果如下表所示：In order to characterize the effect of a humic acid-type mulberry seed coating agent, it was determined according to the specified technology of the national standard GB/T19177-2003 "mulberry seed and seedling inspection procedures". The seeds treated with the humic acid type mulberry seed coating agent of the present invention are the experimental group, and the untreated mulberry seeds are the control group. After culturing for 20 days, count the germination number of seeds every day, and sample and test the germination rate, germination potential, germination index and vigor index of mulberry seeds treated with the humic acid type mulberry seed coating agent of the present invention. The formula is as follows

In the formula, GI represents the germination index, Gt represents the daily germination number, and Dt represents the corresponding germination days; vigor index=average root length×germination index. The usage is described in Example 1 of this patent and used, and the untreated mulberry seeds are routinely fertilized and cultivated, and the specific experimental results are shown in the following table:

The effect of surface humic acid type mulberry seed coating on the vigor of mulberry seeds

sample Germination rate/% Germination potential/% Germination index Vitality Index test group 82.26 73.74 28.62 73.10 control group 72.53 67.50 27.80 54.87

It can be seen from the table that the germination rate, germination potential, germination index and vigor index of mulberry seeds treated with humic acid-type mulberry seed coating agent were improved. Therefore, it is explained that the humic acid type mulberry seed coating agent of the present invention can promote the development of seeds, stimulate the growth and development of the root system of crops, and finally achieve the purpose of increasing production and income.

The present invention has been described in detail with reference to the above-mentioned embodiments, those of ordinary skill in the art should understand that: the specific embodiments of the present invention can still be modified or equivalently replaced, and any modification or equivalent replacement that does not depart from the spirit and scope of the present invention, its shall be covered by the scope of this claim.

Claims (10)

1. a preparation method of humic acid type mulberry seed coating agent, is characterized in that, in parts by weight, comprises the following steps: Step 1: Add 1-3 parts of sodium alginate powder to 100-300 parts of deionized water, stir at room temperature for 4-5 hours to fully swell the sodium alginate; then continue stirring for 0.5-1 hour in a constant temperature water bath at 50-60°C , so that the sodium alginate is fully dissolved, and finally a transparent, uniform and stable sodium alginate aqueous solution is prepared for use; Step 2: Dissolving 1-10 parts of potassium humate in 100-200 parts of deionized water to obtain potassium humate solution; Step 3, taking 5-10 parts of sodium alginate aqueous solution, keeping the temperature in a constant temperature water bath at 50-60 ° C for 10-15 min, then adding 15-20 parts of potassium humate solution, stirring for 20-25 min to obtain a mixed solution; Step 4: Pass nitrogen for 10-15 min into the mixed solution obtained in step 3, and add 10-14 parts of acrylamide monomer and 6-10 parts of acrylic monomer with a neutralization degree of 70% and continue stirring for 10-15 min; Step 5. After the solution is uniform and stable, continue to pass nitrogen for 10-15 minutes to deoxygenate, add 6-8 parts of PEG-400, 0.1-0.2 parts of glycerol, and 0.4-0.6 parts of cross-linking agent, stir for about 2-5 minutes, and finally add 0.1～0.2 part of initiator, at 50～70℃, pass nitrogen for 30～35min to obtain a mixed system; Step 6, under the condition of 50-55 ℃ heat preservation, irradiate the mixed system with ultraviolet light to initiate a polymerization reaction, and react for two hours to obtain a viscous liquid, which is the humic acid type mulberry seed coating agent. 2. the preparation method of humic acid type mulberry seed seed coating agent as claimed in claim 1, is characterized in that, described crosslinking agent is MBA, epichlorohydrin or acrylamide. 3. the preparation method of humic acid type mulberry seed seed coating agent as claimed in claim 1, is characterized in that, described initiator is potassium persulfate, ammonium persulfate or myrcylamine peroxide. 4 . The preparation method of the humic acid type mulberry seed coating agent according to claim 1 , wherein the irradiation wavelength of the ultraviolet lamp is 250-350 nm. 5 . 5. A humic acid type mulberry seed seed coating agent prepared by the method according to any one of claims 1-4. 6. a using method of humic acid type mulberry seed seed coating agent as claimed in claim 5, is characterized in that, adopts artificial seed coating mode to coat mulberry seed. 7. the using method of humic acid type mulberry seed seed coating agent as claimed in claim 6, is characterized in that, comprises the following steps: Step 1: Disinfect, wash and pre-treat the mulberry seeds; Step 2: The pretreated seeds and the humic acid type mulberry seed coating agent are coated according to the mass ratio (20-40): 1, and the seeds are taken out after 4 hours and dried naturally. 8. the using method of humic acid type mulberry seed seed coating agent as claimed in claim 7, is characterized in that, described disinfection method is to immerse mulberry seed in HgCl solution for ₁₅ ～20min. 9 . The method for using the humic acid type mulberry seed coating agent according to claim 8 , wherein the concentration of the HgCl ₂ solution is 0.08-0.1%. 10 . 10. The using method of the humic acid type mulberry seed coating agent as claimed in claim 7, wherein the cleaning method is to rinse with distilled water.

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