CN114569661B - Preparation method of traditional Chinese medicine composition and traditional Chinese medicine composition - Google Patents
Preparation method of traditional Chinese medicine composition and traditional Chinese medicine composition Download PDFInfo
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- CN114569661B CN114569661B CN202011380238.3A CN202011380238A CN114569661B CN 114569661 B CN114569661 B CN 114569661B CN 202011380238 A CN202011380238 A CN 202011380238A CN 114569661 B CN114569661 B CN 114569661B
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Abstract
The invention provides a preparation method of a traditional Chinese medicine composition and the traditional Chinese medicine composition thereof, wherein moutan bark, polygonum multiflorum, phellodendron bark, red paeony root and borneol are crushed into fine powder; soaking fructus Lycii, fructus Ligustri Lucidi, herba Equiseti hiemalis, saviae Miltiorrhizae radix, carthami flos, and flos Eriocauli in water, decocting, filtering, concentrating the filtrate under reduced pressure, precipitating with ethanol, standing, and collecting supernatant as first extractive solution; reflux extracting herba Epimedii, semen Cuscutae, fructus Leonuri, semen Cassiae, corni fructus, rhizoma Ligustici Chuanxiong, rehmanniae radix, and caulis Spatholobi with ethanol, and collecting the second extractive solution; mixing the first extract and the second extract, concentrating the mixed extract, mixing the concentrated thick paste and the fine powder, and granulating to obtain the traditional Chinese medicine composition; the preparation method solves the problems that the preparation in the prior art is uneven in color and luster, and borneol is easy to adsorb on the surfaces of filling equipment and capsule shells by improving the prior art, improves the drug effect of the product in the improvement process, and has stable preparation, obvious curative effect, small toxic and side effects and good market application value.
Description
Technical Field
The invention relates to the field of traditional Chinese medicines. In particular, the invention relates to a preparation method of a traditional Chinese medicine composition and the traditional Chinese medicine composition.
Background
The Chinese medicinal preparation, namely the wolfberry gelatin capsule, prepared from medlar, glossy privet fruit, polygonum multiflorum, borneol and the like for treating eye diseases, myopia, visual fatigue has the effects of tonifying liver and kidney, promoting blood circulation and improving eyesight, and is used for treating symptoms such as eye soreness, orbital pain and the like caused by liver-kidney yin deficiency of teenagers. Borneol is synthesized by chemical method from camphor or pine node oil, or extracted from blumea balsamifera leaf of Compositae, or crystal of borneol resin processed product of borneol. Is a common traditional Chinese medicine, and has the effects of inducing resuscitation, refreshing mind, clearing heat and relieving pain. It is often used for treating febrile diseases such as unconsciousness, convulsion, apoplexy, phlegm syncope, qi stagnation syncope, middle-jiao and malignant coma, chest stuffiness and pain, conjunctival congestion, aphtha, sore throat, and ear canal purulence. It is formulated into pill, powder, tablet, capsule, etc. The borneol has volatility, is easy to sublimate, is ignited to generate dense smoke and has flame with light. Is soluble in ethanol, chloroform, gasoline or diethyl ether, and is hardly soluble in water. Modern researches have shown that borneol has the effects of opening biological barriers of organisms, promoting permeation, relieving pain and inflammation, and relieving pain and dryness.
In the invention patent with the application number of CN20041038251.5, a traditional Chinese medicine for treating eye diseases and myopia and eliminating asthenopia symptoms and a preparation method thereof are disclosed; the invention patent with the application number of CN201610947029.X discloses a preparation method of a Chinese medicine composition of wolfberry; in the invention patent with the application number of CN200710017619.3, a preparation method of a traditional Chinese medicine preparation for treating eye diseases, myopia and asthenopia is disclosed; a prescription composition of QIMING Capsule and its preparation method are disclosed in national pharmaceutical Standard of national food and drug administration. In the prior art, the production process of the wolfberry gelatin capsules is the same, and the borneol is ground into fine powder and is mixed with other medicine fine powder in the recipe, so that the addition mode of the borneol often has the problems that tan particles and powder are white substances, uneven color and luster, insufficient aesthetic degree, and the borneol is easily adsorbed on filling equipment and the surfaces of the capsule shells in the process of filling the capsule shells, and meanwhile, the outer-layer borneol is easily volatilized in the drying process, so that the loss of active ingredients is caused. Meanwhile, other medicinal materials are uniformly decocted, so that the effective components in the traditional Chinese medicine formula cannot be extracted, and the defect of unsatisfactory curative effect of the traditional Chinese medicine formula in the practical application process is overcome.
Therefore, the preparation process of the Qiming capsule still needs to be researched at present.
Disclosure of Invention
The present invention aims to solve at least one of the technical problems existing in the prior art to at least some extent. Therefore, the invention provides a preparation method of a traditional Chinese medicine composition and the traditional Chinese medicine composition, which improves the drug effect of the product, has stable preparation, obvious curative effect and small toxic and side effect through improving the prior art, solves the problems that the color and luster are uneven in the prior art and the borneol is easy to be adsorbed on the surfaces of filling equipment and capsule shells in the improvement process, and has good market application value.
In one aspect of the invention, the invention provides a method for preparing a traditional Chinese medicine composition. According to an embodiment of the present invention, the Chinese medicinal composition is prepared from cortex moutan, polygoni Multiflori radix, cortex Phellodendri, radix Paeoniae Rubra, borneolum Syntheticum, fructus Lycii, fructus Ligustri Lucidi, herba Equiseti hiemalis, saviae Miltiorrhizae radix, carthami flos, flos Eriocauli, herba Epimedii, semen Cuscutae, fructus Leonuri, semen Cassiae, corni fructus, rhizoma Ligustici Chuanxiong, rehmanniae radix and caulis Spatholobi, and the method comprises: pulverizing cortex moutan, polygoni Multiflori radix, cortex Phellodendri, radix Paeoniae Rubra, and Borneolum Syntheticum into fine powder; performing first extraction treatment on the medlar, the glossy privet fruit, the scouring rush, the red sage root, the safflower and the pipewort, and collecting a first extract; performing second extraction treatment on herba epimedii, semen cuscutae, motherwort fruit, semen cassiae, dogwood, ligusticum wallichii, rehmannia root and caulis spatholobi, and collecting a second extract; mixing the first extract and the second extract, and concentrating the mixed extract to obtain thick paste. Mixing the soft extract and the fine powder, and granulating to obtain the traditional Chinese medicine composition.
According to an embodiment of the present invention, the pulverizing the cortex moutan, the polygonum multiflorum, the phellodendron, the red paeony root and the borneol into fine powder comprises: pulverizing cortex moutan, polygoni Multiflori radix, cortex Phellodendri, and radix Paeoniae Rubra into fine powder, sieving, and mixing to obtain first fine powder; pulverizing Borneolum Syntheticum into fine powder, and sieving to obtain second fine powder.
According to the embodiment of the invention, the medlar, the glossy privet fruit, the scouring rush, the red sage root, the safflower and the pipewort are soaked in water, decocted, filtered, the filtrate is decompressed and concentrated, then ethanol is added for precipitation, and the mixture is stood, and the supernatant is collected as a first extract; reflux extracting herba Epimedii, semen Cuscutae, fructus Leonuri, semen Cassiae, corni fructus, rhizoma Ligustici Chuanxiong, rehmanniae radix, and caulis Spatholobi with ethanol, and collecting the second extractive solution.
According to an embodiment of the present invention, before the obtaining of the first fine powder and the obtaining of the second fine powder, the obtaining of the Chinese medicinal composition further comprises: mixing the soft extract with the first fine powder, granulating, drying, sieving with 80 mesh sieve, and sieving to obtain fine powder component and residual granule coarse powder component; incrementally mixing the fine powder component with the second fine powder in equal amount, and uniformly mixing with the residual granular coarse powder component; and (3) transferring the uniformly mixed granular powder into a closed container, standing, spraying ethanol on the surface after standing, uniformly mixing, and airing to obtain the traditional Chinese medicine composition. Therefore, the prepared traditional Chinese medicine composition has uniform color, and the borneol is not easy to adsorb on the surfaces of filling equipment and capsule shells, and the outer-layer borneol is not easy to volatilize in the drying process, so that the component loss is effectively reduced.
According to an embodiment of the invention, the drying conditions are 60-65 ℃ for 2-3 hours, the standing time is 30-40 minutes, and the sprayed alcohol concentration is 95% v/v ethanol.
According to an embodiment of the present invention, the fine powder component and the second fine powder are as follows (0.8 to 1): (1-1.2) and mixing in equal increments.
According to an embodiment of the present invention, the obtaining the first extract includes: decocting fructus Lycii, fructus Ligustri Lucidi, herba Equiseti hiemalis, saviae Miltiorrhizae radix, carthami flos, and flos Eriocauli in water for 3 times, adding water 8-10 times of the weight of the raw materials for the first time, soaking for 30-40 min, adding water 6-8 times of the weight of the raw materials for the second and third times, mixing decoctions, and filtering decoction; concentrating the filtrate under reduced pressure to obtain a thick paste with a relative density of 1.15-1.2 at 60deg.C, adding 95% v/v ethanol to make the ethanol content reach 79% -81% v/v, stirring for 30-40 min, and standing for 12-14 hr to obtain first extractive solution.
According to the embodiment of the invention, the time for each decoction is 1.0-1.2h, and the temperature for each decoction is 95-100 ℃.
According to an embodiment of the present invention, the obtaining the second extract includes: reflux-extracting herba Epimedii, semen Cuscutae, fructus Leonuri, semen Cassiae, corni fructus, rhizoma Ligustici Chuanxiong, rehmanniae radix, and caulis Spatholobi with 75-80% v/v ethanol twice, reflux-extracting with 75-80% v/v ethanol 6-7 times of the weight of the first material, and reflux-extracting with 5-6 times of 75-80% v/v ethanol to obtain the second extractive solution.
According to an embodiment of the invention, the extraction time per reflux is 1.0-1.2 hours, and the extraction temperature at the time of reflux is 75-85 ℃.
According to an embodiment of the present invention, the mixing the first extract and the second extract, and concentrating the mixed extract to obtain a thick paste includes: mixing the first and second extractive solutions, recovering ethanol, and concentrating the mixed extractive solution under reduced pressure to obtain soft extract with relative density of 1.05-1.15 at 60deg.C.
In still another aspect of the present invention, the present invention provides a Chinese medicinal composition prepared by the preparation method, the Chinese medicinal composition comprising: 80-100 parts of medlar, 50-70 parts of moutan bark, 50-70 parts of fleece-flower root, 50-70 parts of phellodendron bark, 50-70 parts of red paeony root, 50-70 parts of glossy privet fruit, 50-70 parts of epimedium herb, 50-70 parts of scouring rush herb, 50-70 parts of red sage root, 50-70 parts of safflower, 50-70 parts of semen cuscutae, 50-70 parts of motherwort fruit, 50-70 parts of dogwood, 50-70 parts of pipewort, 50-70 parts of semen cassiae, 50-70 parts of szechuan lovage rhizome, 50-70 parts of rehmannia root, 50-70 parts of suberect spatholobus stem and 16-20 parts of borneol.
According to an embodiment of the present invention, the Chinese medicinal composition further comprises: pharmaceutically acceptable auxiliary materials.
Additional aspects and advantages of the invention will be set forth in part in the description which follows, and in part will be obvious from the description, or may be learned by practice of the invention.
Detailed Description
Embodiments of the present invention are described in detail below. The following examples are illustrative only and are not to be construed as limiting the invention.
It should be noted that the terms "first," "second," and "second" are used for descriptive purposes only and are not to be construed as indicating or implying a relative importance or implying a number of technical features being indicated. Thus, a feature defining "a first" or "a second" may explicitly or implicitly include one or more such feature. Further, in the description of the present invention, unless otherwise indicated, the meaning of "a plurality" is two or more.
The invention provides a preparation method of a traditional Chinese medicine composition and the composition thereof, and the preparation method and the composition are respectively described in detail below.
Process for the preparation of a composition
In one aspect of the invention, the invention provides a method for preparing a traditional Chinese medicine composition. According to an embodiment of the present invention, the Chinese medicinal composition is prepared from cortex moutan, polygoni Multiflori radix, cortex Phellodendri, radix Paeoniae Rubra, borneolum Syntheticum, fructus Lycii, fructus Ligustri Lucidi, herba Equiseti hiemalis, saviae Miltiorrhizae radix, carthami flos, flos Eriocauli, herba Epimedii, semen Cuscutae, fructus Leonuri, semen Cassiae, corni fructus, rhizoma Ligustici Chuanxiong, rehmanniae radix and caulis Spatholobi, and the method comprises:
s1, pulverizing cortex moutan, radix Polygoni Multiflori, cortex Phellodendri, radix Paeoniae Rubra, and Borneolum Syntheticum into fine powder;
s2, performing first extraction treatment on the medlar, the glossy privet fruit, the scouring rush, the red sage root, the safflower and the pipewort to obtain a first extract;
performing a second extraction treatment on the epimedium, the semen cuscutae, the motherwort fruit, the semen cassiae, the dogwood, the ligusticum wallichii, the rehmannia root and the caulis spatholobi to obtain a second extract;
mixing the first extract and the second extract, and concentrating the mixed extract to obtain thick paste.
S3, mixing the thick paste and the fine powder, and granulating to obtain the traditional Chinese medicine composition.
According to an embodiment of the present invention, the S1 includes: pulverizing cortex moutan, polygoni Multiflori radix, cortex Phellodendri, and radix Paeoniae Rubra into fine powder, sieving with 100 mesh sieve, and mixing to obtain first fine powder; pulverizing Borneolum Syntheticum into fine powder, and sieving with 100 mesh sieve to obtain second fine powder.
According to an embodiment of the present invention, the S2 includes: soaking fructus Lycii, fructus Ligustri Lucidi, herba Equiseti hiemalis, saviae Miltiorrhizae radix, carthami flos, and flos Eriocauli in water, decocting, filtering, concentrating the filtrate under reduced pressure, precipitating with ethanol, standing, and collecting supernatant as first extractive solution; reflux extracting herba Epimedii, semen Cuscutae, fructus Leonuri, semen Cassiae, corni fructus, rhizoma Ligustici Chuanxiong, rehmanniae radix, and caulis Spatholobi with ethanol, and collecting the second extractive solution.
Eight medicinal materials of epimedium herb, semen cuscutae, motherwort fruit, cassia seed, dogwood, szechuan lovage rhizome, rehmannia root and suberect spatholobus stem have poor water extraction effect and less extractum due to the characteristics of the medicinal materials. The extraction time of the main ingredients of the medicinal materials is different from that of other water decoction materials, and the main ingredients of the medicinal materials need to be extracted repeatedly, such as mixed decoction of other medicinal materials, the time is short, the required ingredients cannot be extracted, and the time is long, so that the other medicinal materials in the prescription are boiled, the filtering is difficult, and the normal production is influenced. The eight medicinal materials contain the components of saccharides, sterols, fatty oil, catalpol, glycosides and other compounds, the extraction rate of the compounds in 80% ethanol is highest, the ethanol extraction time is short, and the solvent is easy to recover. Therefore, the method provided by the embodiment of the invention can fully extract the effective components in the medicinal materials, and the effective components can cooperate with each other to play a role, so that the quality of the medicine is improved.
According to an embodiment of the present invention, the S3 includes: mixing the soft extract with the first fine powder, granulating, drying, sieving with 80 mesh sieve, and sieving to obtain fine powder component and residual granule coarse powder component; because the particle size of the second fine powder is greatly different from the particle size of the prepared thick paste and the particle size of the mixed particles of the first fine powder, and the amount ratio is also greatly different, and the mixture is difficult to be uniformly mixed, part of fine powder in the particles is screened out as a diluent of the second fine powder, and the mixture is easy to be uniformly mixed because the particle size of the second fine powder and the particle size of the mixed particles are not greatly different, and the fine powder component and the second fine powder are gradually mixed in equal amount and are uniformly mixed with the rest of coarse powder components; the evenly mixed granular powder is transferred to a closed container for standing, and after standing, the borneol can fully permeate into capillary holes in the granules, so that the adsorption force between the borneol and the granules is enhanced, and the volatilization loss of the borneol can be inhibited. And (3) spraying ethanol on the surface of the borneol after standing, uniformly mixing and airing to obtain the traditional Chinese medicine composition. Therefore, the prepared traditional Chinese medicine composition has uniform color, and the borneol is not easy to adsorb on the surfaces of filling equipment and capsule shells, and the outer-layer borneol is not easy to volatilize in the drying process, so that the component loss is effectively reduced.
According to an embodiment of the invention, the drying conditions are 60-65 ℃ for 2-3 hours, the standing time is 30-40 minutes, and the sprayed alcohol concentration is 95% v/v ethanol.
According to an embodiment of the present invention, the fine powder component and the second fine powder are as follows (0.8 to 1): (1-1.2) and mixing in equal increments.
According to an embodiment of the present invention, the obtaining the first extract includes: decocting fructus Lycii, fructus Ligustri Lucidi, herba Equiseti hiemalis, saviae Miltiorrhizae radix, carthami flos, and flos Eriocauli in water for 3 times, adding water 8-10 times of the weight of the raw materials for the first time, soaking for 30-40 min, adding water 6-8 times of the weight of the raw materials for the second and third times, mixing decoctions, and filtering decoction; concentrating the filtrate under reduced pressure to obtain a thick paste with a relative density of 1.15-1.2 at 60deg.C, adding 95% v/v ethanol to make the ethanol content reach 79% -81% v/v, stirring for 30-40 min, and standing for 12-14 hr to obtain first extractive solution.
According to the embodiment of the invention, the time for each decoction is 1.0-1.2h, and the temperature for each decoction is 95-100 ℃.
According to an embodiment of the present invention, the obtaining the second extract includes: reflux-extracting herba Epimedii, semen Cuscutae, fructus Leonuri, semen Cassiae, corni fructus, rhizoma Ligustici Chuanxiong, rehmanniae radix, and caulis Spatholobi with 75-80% v/v ethanol twice, reflux-extracting with 75-80% v/v ethanol 6-7 times of the weight of the first material, and reflux-extracting with 5-6 times of 80% v/v ethanol to obtain the second extractive solution.
According to an embodiment of the invention, the extraction time per reflux is 1.0-1.2 hours, and the extraction temperature at the time of reflux is 75-85 ℃.
According to an embodiment of the present invention, the mixing the first extract and the second extract, and concentrating the mixed extract to obtain a thick paste includes: mixing the first and second extractive solutions, recovering ethanol, and concentrating the mixed extractive solution under reduced pressure to obtain soft extract with relative density of 1.05-1.15 at 60deg.C.
Medicament
In yet another aspect of the invention, the invention provides a pharmaceutical composition. According to an embodiment of the present invention, the pharmaceutical composition is prepared by the preparation method, and the Chinese medicinal composition comprises: 80-100 parts of medlar, 50-70 parts of moutan bark, 50-70 parts of fleece-flower root, 50-70 parts of phellodendron bark, 50-70 parts of red paeony root, 50-70 parts of glossy privet fruit, 50-70 parts of epimedium herb, 50-70 parts of scouring rush herb, 50-70 parts of red sage root, 50-70 parts of safflower, 50-70 parts of semen cuscutae, 50-70 parts of motherwort fruit, 50-70 parts of dogwood, 50-70 parts of pipewort, 50-70 parts of semen cassiae, 50-70 parts of szechuan lovage rhizome, 50-70 parts of rehmannia root, 50-70 parts of suberect spatholobus stem and 16-20 parts of borneol.
According to an embodiment of the present invention, the pharmaceutical composition further comprises: pharmaceutically acceptable auxiliary materials. Therefore, the compound is helpful for better playing the drug effect and improving the flavor, taste and stability.
The scheme of the present invention will be explained below with reference to examples. It will be appreciated by those skilled in the art that the following examples are illustrative of the present invention and should not be construed as limiting the scope of the invention. The examples are not to be construed as limiting the specific techniques or conditions described in the literature in this field or as per the specifications of the product. The reagents or apparatus used were conventional products commercially available without the manufacturer's attention.
Example 1A method for preparing a Chinese medicinal composition
Weighing 90g of medlar, 60g of tree peony bark, 60g of tuber fleeceflower root, 60g of amur corktree bark, 60g of red paeony root, 60g of glossy privet fruit, 60g of epimedium herb, 60g of common scouring rush herb, 60g of red sage root, 60g of safflower, 60g of Chinese dodder seed, 60g of motherwort fruit, 60g of common macrocarpium fruit, 60g of pipewort herb, 60g of cassia seed, 60g of szechuan lovage rhizome, 60g of rehmannia root, 60g of suberect spatholobus stem and 18g of borneol;
pulverizing cortex moutan, polygoni Multiflori radix, cortex Phellodendri, radix Paeoniae Rubra, and Borneolum Syntheticum into fine powder, and sieving with 100 mesh sieve;
decocting fructus Lycii, fructus Ligustri Lucidi, herba Equiseti hiemalis, saviae Miltiorrhizae radix, carthami flos, and flos Eriocauli in water for three times, wherein the first time is 10 times of water, soaking for 30min, decocting for 1 hr, and the second and third times are 8 times of water, respectively, decocting for 1 hr; mixing decoctions, filtering, concentrating the filtrate under reduced pressure to obtain soft extract with relative density of 1.15 at 60deg.C, adding 95% ethanol to 80%, stirring for 30min, and standing for 12 hr to obtain first extractive solution;
reflux-extracting herba Epimedii, semen Cuscutae, fructus Leonuri, semen Cassiae, corni fructus, rhizoma Ligustici Chuanxiong, rehmanniae radix, and caulis Spatholobi with 80% ethanol twice, wherein the first time is 6 times of the weight of the raw materials, reflux-extracting for 1 hr, and the second time is 5 times of the weight of the raw materials, reflux-extracting for 1 hr to obtain second extractive solution; mixing the first extract and the second extract, recovering ethanol, and concentrating the obtained mixture under reduced pressure to obtain soft extract with relative density of 1.05 at 60deg.C;
mixing the soft extract with the fine powder, granulating, drying at 60deg.C for 2 hr, and grading.
Example 2A method for preparing a Chinese medicinal composition
Weighing 90g of medlar, 60g of tree peony bark, 60g of tuber fleeceflower root, 60g of amur corktree bark, 60g of red paeony root, 60g of glossy privet fruit, 60g of epimedium herb, 60g of common scouring rush herb, 60g of red sage root, 60g of safflower, 60g of Chinese dodder seed, 60g of motherwort fruit, 60g of common macrocarpium fruit, 60g of pipewort herb, 60g of cassia seed, 60g of szechuan lovage rhizome, 60g of rehmannia root, 60g of suberect spatholobus stem and 18g of borneol;
pulverizing cortex moutan, polygoni Multiflori radix, cortex Phellodendri, and radix Paeoniae Rubra into fine powder, sieving with 100 mesh sieve, and mixing to obtain first fine powder; pulverizing Borneolum Syntheticum into fine powder, and sieving with 100 mesh sieve to obtain second fine powder;
decocting fructus Lycii, fructus Ligustri Lucidi, herba Equiseti hiemalis, saviae Miltiorrhizae radix, carthami flos, and flos Eriocauli in water for three times, wherein the first time is 10 times of water, soaking for 30min, decocting for 1 hr, and the second and third times are 8 times of water, respectively, decocting for 1 hr; mixing decoctions, filtering, concentrating the filtrate under reduced pressure to obtain soft extract with relative density of 1.15 at 60deg.C, adding 92% ethanol to 80%, stirring for 30min, and standing for 12 hr to obtain first extractive solution;
reflux-extracting herba Epimedii, semen Cuscutae, fructus Leonuri, semen Cassiae, corni fructus, rhizoma Ligustici Chuanxiong, rehmanniae radix, and caulis Spatholobi with 80% ethanol twice, wherein the first time is 6 times of the weight of the raw materials, reflux-extracting for 1 hr, and the second time is 5 times of the weight of the raw materials, reflux-extracting for 1 hr to obtain second extractive solution; mixing the first extract and the second extract, recovering ethanol, and concentrating the obtained mixture under reduced pressure to obtain soft extract with relative density of 1.05 at 60deg.C;
mixing the soft extract with the first fine powder, granulating, drying at 65deg.C for 3 hr, grading, adding the second fine powder, and mixing.
Example 3A method for preparing a Chinese medicinal composition
Weighing 90g of medlar, 60g of tree peony bark, 60g of tuber fleeceflower root, 60g of amur corktree bark, 60g of red paeony root, 60g of glossy privet fruit, 60g of epimedium herb, 60g of common scouring rush herb, 60g of red sage root, 60g of safflower, 60g of Chinese dodder seed, 60g of motherwort fruit, 60g of common macrocarpium fruit, 60g of pipewort herb, 60g of cassia seed, 60g of szechuan lovage rhizome, 60g of rehmannia root, 60g of suberect spatholobus stem and 18g of borneol;
pulverizing cortex moutan, polygoni Multiflori radix, cortex Phellodendri, and radix Paeoniae Rubra into fine powder, sieving with 100 mesh sieve, and mixing to obtain first fine powder; pulverizing Borneolum Syntheticum into fine powder, and sieving with 100 mesh sieve to obtain second fine powder;
decocting fructus Lycii, fructus Ligustri Lucidi, herba Equiseti hiemalis, saviae Miltiorrhizae radix, carthami flos, and flos Eriocauli in water for three times, wherein the first time is 10 times of water, soaking for 30min, decocting for 1 hr, and the second and third times are 8 times of water, respectively, decocting for 1 hr; mixing decoctions, filtering, concentrating the filtrate under reduced pressure to obtain soft extract with relative density of 1.15 at 60deg.C, adding 95% ethanol to 80%, stirring for 30min, and standing for 12 hr to obtain first extractive solution;
reflux-extracting herba Epimedii, semen Cuscutae, fructus Leonuri, semen Cassiae, corni fructus, rhizoma Ligustici Chuanxiong, rehmanniae radix, and caulis Spatholobi with 80% ethanol twice, wherein the first time is 6 times of the weight of the raw materials, reflux-extracting for 1 hr, and the second time is 5 times of the weight of the raw materials, reflux-extracting for 1 hr to obtain second extractive solution; mixing the first extract and the second extract, recovering ethanol, and concentrating the obtained mixture under reduced pressure to obtain soft extract with relative density of 1.05 at 60deg.C;
mixing the soft extract with the first fine powder, granulating, drying at 60deg.C for 2 hr, sieving with 80 mesh sieve, and sieving to obtain fine powder component and residual granule coarse powder component; mixing the fine powder component with the second fine powder in an equal and incremental way according to the proportion of 1:1.2, and uniformly mixing with the rest granule coarse powder component; and (3) transferring the uniformly mixed granular powder into a closed container, and sealing for 30 minutes, spraying 95% ethanol on the surface of the sealed component, uniformly mixing, and airing to obtain the traditional Chinese medicine composition.
Example 4A method for preparing a Chinese medicinal composition
Weighing 80g of medlar, 50g of tree peony bark, 50g of tuber fleeceflower root, 50g of amur corktree bark, 50g of red paeony root, 50g of glossy privet fruit, 50g of epimedium herb, 50g of scouring rush herb, 50g of red sage root, 50g of safflower, 50g of Chinese dodder seed, 50g of motherwort fruit, 50g of common macrocarpium fruit, 50g of pipewort herb, 50g of cassia seed, 50g of szechuan lovage rhizome, 50g of rehmannia root, 50g of suberect spatholobus stem and 16g of borneol.
Pulverizing cortex moutan, polygoni Multiflori radix, cortex Phellodendri, and radix Paeoniae Rubra into fine powder, sieving with 100 mesh sieve, and mixing to obtain first fine powder; pulverizing Borneolum Syntheticum into fine powder, and sieving with 100 mesh sieve to obtain second fine powder;
decocting fructus Lycii, fructus Ligustri Lucidi, herba Equiseti hiemalis, saviae Miltiorrhizae radix, carthami flos, and flos Eriocauli in water for three times, wherein the first time is soaking in 8 times of water, decocting for 1.2 hr, and the second and third times are each decocting in 6 times of water for 1.2 hr; mixing decoctions, filtering, concentrating the filtrate under reduced pressure to obtain soft extract with relative density of 1.2 at 60deg.C, adding 95% ethanol to make the ethanol content reach 79%, stirring for 40 min, and standing for 13 hr to obtain first extractive solution;
reflux-extracting herba Epimedii, semen Cuscutae, fructus Leonuri, semen Cassiae, corni fructus, rhizoma Ligustici Chuanxiong, rehmanniae radix, and caulis Spatholobi with 80% ethanol twice, wherein the first time is 6.5 times of the weight of the raw materials, reflux-extracting for 1.1 hr, and the second time is 5 times of the weight of the raw materials, reflux-extracting for 1 hr to obtain second extractive solution; mixing the first extract and the second extract, recovering ethanol, and concentrating the obtained mixture under reduced pressure to obtain soft extract with relative density of 1.05 at 60deg.C;
mixing the soft extract with the first fine powder, granulating, drying at 60deg.C for 2 hr, sieving with 80 mesh sieve, and sieving to obtain fine powder component and residual granule coarse powder component; mixing the fine powder component with the second fine powder according to the ratio of 0.8:1, mixing the mixture in an equal and incremental way, sieving the mixture, and uniformly mixing the mixture with the rest coarse powder components; and (3) transferring the uniformly mixed granular powder into a closed container, and sealing for 35 minutes, spraying 95% ethanol on the surface of the sealed component, uniformly mixing, and airing to obtain the traditional Chinese medicine composition.
Example 5A method for preparing a Chinese medicinal composition
Weighing 100g of medlar, 70g of tree peony bark, 70g of tuber fleeceflower root, 70g of amur corktree bark, 70g of red paeony root, 70g of glossy privet fruit, 70g of epimedium herb, 70g of common scouring rush herb, 70g of red sage root, 70g of safflower, 70g of Chinese dodder seed, 70g of motherwort fruit, 70g of common macrocarpium fruit, 70g of pipewort herb, 70g of cassia seed, 70g of szechuan lovage rhizome, 70g of rehmannia root, 70g of suberect spatholobus stem and 20g of borneol;
pulverizing cortex moutan, polygoni Multiflori radix, cortex Phellodendri, and radix Paeoniae Rubra into fine powder, sieving with 100 mesh sieve, and mixing to obtain first fine powder; pulverizing Borneolum Syntheticum into fine powder, and sieving with 100 mesh sieve to obtain second fine powder;
decocting fructus Lycii, fructus Ligustri Lucidi, herba Equiseti hiemalis, saviae Miltiorrhizae radix, carthami flos, and flos Eriocauli in water for three times, wherein the first time is 9 times of water, soaking for 35 min, decocting for 1.1 hr, and the second and third times are each 7 times of water, decocting for 1.1 hr; mixing decoctions, filtering, concentrating the filtrate under reduced pressure to obtain soft extract with relative density of 1.15 at 60deg.C, adding 95% ethanol to make the ethanol content reach 81%, stirring for 40 min, and standing for 14 hr to obtain first extractive solution;
reflux-extracting herba Epimedii, semen Cuscutae, fructus Leonuri, semen Cassiae, corni fructus, rhizoma Ligustici Chuanxiong, rehmanniae radix, and caulis Spatholobi with 80% ethanol twice, wherein the first time is 7 times of the weight of the raw materials, reflux-extracting for 1.2 hr, and the second time is 6 times of the weight of the raw materials, reflux-extracting for 1.2 hr to obtain second extractive solution; mixing the first extract and the second extract, recovering ethanol, and concentrating the obtained mixture under reduced pressure to obtain soft extract with relative density of 1.15 at 60deg.C;
mixing the soft extract with the first fine powder, granulating, drying at 60deg.C for 2 hr, sieving with 80 mesh sieve, and sieving to obtain fine powder component and residual granule coarse powder component; mixing the fine powder component with a second fine powder according to a ratio of 1:1, mixing the mixture in an equal and incremental way, sieving the mixture, and uniformly mixing the mixture with the rest coarse powder components; and (3) transferring the uniformly mixed granular powder into a closed container, and sealing for 40 minutes, spraying 95% ethanol on the surface of the sealed component, uniformly mixing, and airing to obtain the traditional Chinese medicine composition.
Comparative example 1 one-time mixing of Borneolum Syntheticum, decocting the medicinal materials with water
A composition prepared according to the method of example 1, specification of the patent application CN 20041038251.5.
Weighing 90g of medlar, 60g of tree peony bark, 60g of tuber fleeceflower root, 60g of amur corktree bark, 60g of red paeony root, 60g of glossy privet fruit, 60g of epimedium herb, 60g of common scouring rush herb, 60g of red sage root, 60g of safflower, 60g of Chinese dodder seed, 60g of motherwort fruit, 60g of common macrocarpium fruit, 60g of pipewort herb, 60g of cassia seed, 60g of szechuan lovage rhizome, 60g of rehmannia root, 60g of suberect spatholobus stem and 18g of borneol;
grinding Borneolum Syntheticum in a mortar, and sieving with 80 mesh sieve;
mixing cortex moutan, radix Polygoni Multiflori Preparata, cortex Phellodendri, radix Paeoniae Rubra, and pulverizing into 80-120 mesh fine powder;
decocting the other fourteen medicines in water for three times, wherein the first time is 8 times of water, soaking for 30 minutes, decocting for 1 hour, the second and third times are 6 times of water, decocting for 1 hour, mixing decoctions, and filtering;
concentrating the filtrate under reduced pressure to obtain soft extract with relative density of 1.20 at 60deg.C, adding the above fine powder prepared in step S3, mixing, granulating, oven drying at 60deg.C, and grading; adding the fine powder prepared in the step S2, and uniformly mixing to obtain the final product.
Comparative example 2 one-time mixing of Borneolum Syntheticum, reflux extraction of medicinal materials with alcohol
Weighing 90g of medlar, 60g of tree peony bark, 60g of tuber fleeceflower root, 60g of amur corktree bark, 60g of red paeony root, 60g of glossy privet fruit, 60g of epimedium herb, 60g of common scouring rush herb, 60g of red sage root, 60g of safflower, 60g of Chinese dodder seed, 60g of motherwort fruit, 60g of common macrocarpium fruit, 60g of pipewort herb, 60g of cassia seed, 60g of szechuan lovage rhizome, 60g of rehmannia root, 60g of suberect spatholobus stem and 18g of borneol;
pulverizing cortex moutan, polygoni Multiflori radix, cortex Phellodendri, and radix Paeoniae Rubra into fine powder, sieving with 100 mesh sieve, and mixing to obtain first fine powder; pulverizing Borneolum Syntheticum into fine powder, and sieving with 100 mesh sieve to obtain second fine powder;
reflux-extracting the rest fourteen materials with 80% ethanol twice, wherein the first time is 6 times of the weight of the materials, reflux-extracting for 1 hr, the second time is 5 times of the weight of the materials, reflux-extracting for 1 hr, mixing the reflux liquids, recovering ethanol, and concentrating the obtained reflux liquid under reduced pressure to obtain soft extract with relative density of 1.05 at 60deg.C;
mixing the soft extract with the first fine powder, granulating, drying at 60deg.C for 2 hr, grading, adding the second fine powder, and mixing.
Comparative example 3 mixing Borneolum Syntheticum in portions, decocting the herbs in water and extracting
Weighing 90g of medlar, 60g of tree peony bark, 60g of tuber fleeceflower root, 60g of amur corktree bark, 60g of red paeony root, 60g of glossy privet fruit, 60g of epimedium herb, 60g of common scouring rush herb, 60g of red sage root, 60g of safflower, 60g of Chinese dodder seed, 60g of motherwort fruit, 60g of common macrocarpium fruit, 60g of pipewort herb, 60g of cassia seed, 60g of szechuan lovage rhizome, 60g of rehmannia root, 60g of suberect spatholobus stem and 18g of borneol;
pulverizing cortex moutan, polygoni Multiflori radix, cortex Phellodendri, and radix Paeoniae Rubra into fine powder, sieving with 100 mesh sieve, and mixing to obtain first fine powder; pulverizing Borneolum Syntheticum into fine powder, and sieving with 100 mesh sieve to obtain second fine powder;
decocting the other fourteen medicines in water for three times, wherein the first time is 10 times of water, soaking for 30 minutes, decocting for 1 hour, the second and third times are 8 times of water, decocting for 1 hour, mixing decoctions, and filtering; concentrating the filtrate under reduced pressure to obtain soft extract with relative density of 1.20 and temperature of 60deg.C;
mixing the soft extract with the first fine powder, granulating, drying at 60deg.C for 2 hr, sieving with 80 mesh sieve, and sieving to obtain fine powder component and residual granule coarse powder component; mixing the fine powder component in the sieved particles with the second fine powder in an equal amount and increasing the ratio of 1:1.2, and uniformly mixing with the coarse powder component of the residual particles; and (3) transferring the uniformly mixed granular powder into a closed container, and sealing for 30 minutes, spraying 95% ethanol on the surface of the sealed component, uniformly mixing, and airing to obtain the traditional Chinese medicine composition.
Comparative example 4 borneol is mixed in several times, and the medicinal material is extracted by reflux with alcohol
Weighing 90g of medlar, 60g of tree peony bark, 60g of tuber fleeceflower root, 60g of amur corktree bark, 60g of red paeony root, 60g of glossy privet fruit, 60g of epimedium herb, 60g of common scouring rush herb, 60g of red sage root, 60g of safflower, 60g of Chinese dodder seed, 60g of motherwort fruit, 60g of common macrocarpium fruit, 60g of pipewort herb, 60g of cassia seed, 60g of szechuan lovage rhizome, 60g of rehmannia root, 60g of suberect spatholobus stem and 18g of borneol;
pulverizing cortex moutan, polygoni Multiflori radix, cortex Phellodendri, and radix Paeoniae Rubra into fine powder, sieving with 100 mesh sieve, and mixing to obtain first fine powder; pulverizing Borneolum Syntheticum into fine powder, and sieving with 100 mesh sieve to obtain second fine powder;
reflux-extracting the rest fourteen materials with 80% ethanol twice, wherein the first time is 6 times of the weight of the materials, reflux-extracting for 1 hr, the second time is 5 times of the weight of the materials, reflux-extracting for 1 hr, mixing the reflux liquids, recovering ethanol, and concentrating the obtained reflux liquid under reduced pressure to obtain soft extract with relative density of 1.05 at 60deg.C;
mixing the soft extract with the first fine powder, granulating, drying at 60deg.C for 2 hr, sieving with 80 mesh sieve, and sieving to obtain fine powder component and residual granule coarse powder component; mixing the fine powder component with the second fine powder in an equal and incremental way according to the proportion of 1:1.2, and uniformly mixing with the rest granule coarse powder component; and (3) transferring the uniformly mixed granular powder into a closed container, and sealing for 30 minutes, spraying 95% ethanol on the surface of the sealed component, uniformly mixing, and airing to obtain the traditional Chinese medicine composition.
Functional test
1. Preparation of experimental medicine
1. Preparation of raw materials:
a Chinese medicinal composition is prepared from fructus Lycii 90g, cortex moutan 60g, polygoni Multiflori radix 60g, cortex Phellodendri 60g, radix Paeoniae Rubra 60g, fructus Ligustri Lucidi 60g, herba Epimedii 60g, herba Equiseti hiemalis 60g, saviae Miltiorrhizae radix 60g, carthami flos 60g, semen Cuscutae 60g, fructus Leonuri 60g, corni fructus 60g, flos Eriocauli 60g, semen Cassiae 60g, rhizoma Ligustici Chuanxiong 60g, rehmanniae radix 60g, caulis Spatholobi 60g and Borneolum 18g; corresponding pharmaceutical formulations were prepared according to the methods of example 1, example 2, example 3, comparative example 1, comparative example 2, comparative example 3, comparative example 4, respectively, of the present invention. Wherein:
group I is: prepared according to the method of example 1 of the specification.
Group II is: prepared according to the method of example 2 of the specification.
Group III is: prepared according to the method of example 3 of the present specification.
The group A is as follows: a composition prepared according to the method of comparative example 1.
The group B is as follows: a composition prepared according to the method of comparative example 2.
Group C is: a composition prepared according to the method of comparative example 3.
The group D is: a composition prepared according to the method of comparative example 4.
2. Test procedure and test results
2.1, experimental purposes:
pharmacological experiments of the group I, the group II, the group III and the group A, B, C, D for resisting ciliary muscle spasm, promoting conjunctival microcirculation, improving auricle microcirculation, inhibiting capillary permeability and the like are studied, and the pharmacological effects of the group I, the group II, the group III and the group A, B, C, D are compared to observe.
2.2, test method:
influence of groups I, II, III and A, B, C, D on rabbit isolated ciliary muscle;
influence of group I, group II, group III and group A, B, C, D on rabbit microcirculation;
effects of groups I, II, III and A, B, C, D on capillary permeability.
2.3 effects on rabbit isolated ciliary muscle:
6 rabbits were selected, the eyes were dissected immediately after brain death by wooden cradling, the ciliary muscle was gently removed and placed in a DC-001 type isolated organ tester with Taiwan fluid, and ventilation was maintained at 37 ℃. One end is tied on the lifting hook, and the other end is arranged on the transducer hook. After recording a normal contraction curve, drugs were sequentially added to the table fluid and the amplitude of ciliary muscle contraction was recorded. Adding 1×10 -4 g/ml acetylcholine 0.05ml, and the contraction amplitude and tension immediately increased. When the shrinkage reaches the peak value, the concentration is added to be 2.0x10 -2 group I drug 0.2ml g/ml, observed ciliary muscle contraction amplitude and tension, and the same method was used to continue 6 groups of experiments, each group added at a concentration of 2.0X10 -2 g/ml A, B, C, D group drug 0.2ml, ciliary muscle contraction amplitude and tension were observed and the results are detailed in Table 1.
TABLE 1 Effect of acetylcholine induced ciliary muscle spasms
Compared with the acetylcholine group △ P is less than 0.05; ratio to group I a P is less than 0.05; group II ratio b P is less than 0.05; group III ratio c As can be seen from the results of Table 1, the addition concentration was 2.0X10 -2 After 0.2ml of g/ml of group I, group II, group III and A, B, C, D, the amplitude and tension of ciliary muscle contraction were significantly reduced, and the group I, group II, group III and A, B, C, D were significantly reduced compared with the acetylcholine groupStatistical differences were noted (P < 0.05), and differences between groups I, II, III and the other test groups were all statistically significant (P < 0.05). Therefore, the medicines in the groups I, II, III and A, B, C, D have obvious antagonism on the ciliary muscle spasmodic contraction caused by the acetylcholine, and the antagonism of the medicines in the groups I, II and III is stronger than that of the medicines in the group A, B, C, D, wherein the group III can obtain the optimal pharmacological effect.
2.4 influence on rabbit microcirculation:
2.4.1 effects on rabbit ocular conjunctival microcirculation: rabbit eye conjunctiva microvascular fluorescence assay was used. 48 rabbits are taken, the weight of the rabbits is 2.8-3.0kg, and the rabbits are randomly divided into 8 groups. The drugs of the control group (normal saline), the group I, the group II, the group III and the group A, B, C, D are respectively administrated by being infused into the stomach, 1 time a day for 7 days. 1h after the last administration, ear 1V10% sodium fluorescein physiological saline solution 100mg/kg, and record rabbit eye conjunctiva fluorescence time, the results are shown in Table 2.
TABLE 2 influence on rabbit eye conjunctival microcirculation
| Grouping | Dosage of | Number of animals | Eye conjunctiva fluorescence appearance time(s) |
| Control group | 1ml/kg | 6 | 14.05±0.35 abc |
| Group I | 2.4g/kg | 6 | 10.48±0.48 △ |
| Group II | 2.4g/kg | 6 | 10.43±0.42 △ |
| Group III | 2.4g/kg | 6 | 10.36±0.44 △ |
| Group A | 2.4g/kg | 6 | 13.02±0.48 △abc |
| Group B | 2.4g/kg | 6 | 12.15±0.87 △abc |
| Group C | 2.4g/kg | 6 | 12.87±0.52 △abc |
| Group D | 2.4g/kg | 6 | 12.82±1.14 △abc |
Compared with the control group △ P is less than 0.05; ratio to group I a P is less than 0.05; group II ratio b P is less than 0.05; group III ratio c P＜0.05
As can be seen from the results in Table 2, the groups I, II, III and A, B, C, D have significant statistical differences (P < 0.05) compared with the control group, and the differences among the groups I, II, III and the other test groups have statistical significance (P < 0.05); therefore, the medicines in groups I, II, III and A, B, C, D all have the effect of promoting the conjunctiva microcirculation of the rabbit eyes, and the groups I, II and III have stronger effect than the group A, B, C, D, wherein the group III can obtain the optimal pharmacological effect.
2.4.2 effects on mouse auricle microcirculation:
80 mice were randomly divided into 8 groups. 10' after administration by gavage at the dose of Table 3, 10ug/kg of ip epinephrine. Then, 0.05ml/l of 1% pentobarbital sodium solution and 0g ip were used for anesthesia, the mice were fixed on the rat plates in a prone position, 1 plastic cap was placed under the auricle, the auricle was placed in a horizontal position, and the dorsal fuzz was cut off and observed under an epilight source with a microscope for 30 minutes after administration, and the effect of the drug on microcirculation disturbance caused by epinephrine was observed. The results are detailed in Table 3.
TABLE 3 influence on the caliber of auricle vessels
Compared with the control group △ P is less than 0.05; ratio to group I a P is less than 0.05; group II ratio b P is less than 0.05; group III ratio c P＜0.05
As can be seen from the results in Table 3, the groups I, II, III and A, B, C, D have significant statistical differences (P < 0.05) compared with the control group, and the differences among the groups I, II, III and the other test groups have statistical significance (P < 0.05); therefore, the medicines in groups I, II, III and A, B, C, D can be used for resisting microcirculation disturbance caused by epinephrine, obviously increasing the diameter of micro-motion and veins and improving microcirculation, and the medicines in groups I, II and III have stronger effect than those in group A, B, C, D, wherein the group III can obtain the optimal pharmacological effect.
2.5 Effect on capillary permeability
80 mice were randomly divided into 8 groups. The doses are given as in table 4, 1 time daily for 7 consecutive days. After 1h of final administration, 10ml/kg of 1% Evans orchid and 10ml/kg of ip0.5% acetic acid were injected into the tail vein. After 30min, the mice were sacrificed, 5ml of distilled water was injected into the abdominal cavity to wash the abdominal cavity, and the abdominal cavity wash solution was collected and colorimetrized at 590nm to determine the absorbance. The data were t-checked and are presented in Table 4.
TABLE 4 influence on the permeability of capillaries in the abdominal cavity
Compared with the control group △ P is less than 0.05; ratio to group I a P is less than 0.05; group II ratio b P is less than 0.05; group III ratio c P＜0.05
As can be seen from the results in Table 4, the groups I, II, III and A, B, C, D have significant statistical differences (P < 0.05) compared with the control group, and the differences among the groups I, II, III and the other test groups have statistical significance (P < 0.05); therefore, the medicines in groups I, II, III and A, B, C, D have obvious inhibition effect on the increase of the capillary permeability of the abdominal cavity caused by acetic acid, and the inhibition effect of groups I, II and III is stronger than that of group A, B, C, D, wherein the group III can obtain the optimal pharmacological effect.
In the description of the present specification, a description referring to terms "one embodiment," "some embodiments," "examples," "specific examples," or "some examples," etc., means that a particular feature, structure, material, or characteristic described in connection with the embodiment or example is included in at least one embodiment or example of the present invention. In this specification, schematic representations of the above terms are not necessarily directed to the same embodiment or example. Furthermore, the particular features, structures, materials, or characteristics described may be combined in any suitable manner in any one or more embodiments or examples. Furthermore, the different embodiments or examples described in this specification and the features of the different embodiments or examples may be combined and combined by those skilled in the art without contradiction.
While embodiments of the present invention have been shown and described above, it will be understood that the above embodiments are illustrative and not to be construed as limiting the invention, and that variations, modifications, alternatives and variations may be made to the above embodiments by one of ordinary skill in the art within the scope of the invention.
Claims (2)
1. The preparation method of the traditional Chinese medicine composition comprises 60g of tree peony bark, 60g of tuber fleeceflower root, 60g of amur corktree bark, 60g of red paeony root, 18g of borneol, 90g of medlar, 60g of glossy privet fruit, 60g of common scouring rush herb, 60g of red sage root, 60g of safflower, 60g of pipewort herb, 60g of epimedium herb, 60g of Chinese dodder seed, 60g of motherwort fruit, 60g of cassia seed, 60g of common macrocarpium fruit, 60g of szechuan lovage rhizome, 60g of rehmannia root and 60g of suberect spatholobus stem, and is characterized by comprising the following steps:
s1, pulverizing cortex moutan, polygonum multiflorum, phellodendron bark and red paeony root into fine powder, sieving with a 100-mesh sieve, and uniformly mixing to obtain first fine powder; pulverizing Borneolum Syntheticum into fine powder, and sieving with 100 mesh sieve to obtain second fine powder;
s2, decocting the medlar, the glossy privet fruit, the scouring rush, the red sage root, the safflower and the pipewort in water for 3 times, wherein the water with the weight of 8-10 times of the weight of the raw materials is added for soaking for 30-40 minutes in the first time, and the water with the weight of 6-8 times of the weight of the raw materials is added for the second time and the third time respectively, and filtering the decoction after merging the decoctions;
concentrating the filtrate under reduced pressure to obtain a thick paste with a relative density of 1.15-1.2 at 60 ℃, adding 95% v/v ethanol into the thick paste to ensure that the ethanol content reaches 79% -81% v/v, stirring for 30-40 min, and standing for 12-14 hours to obtain a first extract;
the time for each decoction is 1.0-1.2h, and the temperature for each decoction is 95-100deg.C;
reflux extracting herba Epimedii, semen Cuscutae, fructus Leonuri, semen Cassiae, corni fructus, rhizoma Ligustici Chuanxiong, rehmanniae radix, and caulis Spatholobi with 75-80% v/v ethanol twice, reflux extracting with 75-80% v/v ethanol 6-7 times of the weight of the first material, reflux extracting with 5-6 times of 75-80% v/v ethanol to obtain second extractive solution;
the extraction time of each reflux is 1.0-1.2h, and the extraction temperature during the reflux is 75-85 ℃;
mixing the first extractive solution and the second extractive solution, recovering ethanol, concentrating the obtained mixed extractive solution under reduced pressure to obtain soft extract with relative density of 1.05-1.15 at 60deg.C;
s3, mixing the thick paste with the first fine powder, granulating, drying, sieving, and sieving out the fine powder component and the residual granule coarse powder component in the granules;
incrementally mixing the fine powder component with the second fine powder in equal amount, and uniformly mixing with the residual granular coarse powder component;
transferring the uniformly mixed granular powder into a closed container, standing, spraying ethanol on the surface after standing, uniformly mixing, and airing to obtain the traditional Chinese medicine composition;
the drying condition is that the drying is carried out for 2h to 3h at the temperature of 60 ℃ to 65 ℃, the standing time is 30 minutes to 40 minutes, and the concentration of the sprayed alcohol is 95 percent v/v ethanol;
the fine powder component and the second fine powder are mixed according to the following proportion of (0.8-1): (1-1.2) in an equal incremental mixing.
2. A Chinese medicinal composition prepared according to the preparation method of claim 1.
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