CN114558629B - Microfluidic type thrombus elasticity analysis and detection kit - Google Patents
Microfluidic type thrombus elasticity analysis and detection kit Download PDFInfo
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- 238000001514 detection method Methods 0.000 title claims abstract description 78
- 208000007536 Thrombosis Diseases 0.000 title claims abstract description 32
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- 239000003153 chemical reaction reagent Substances 0.000 claims abstract description 189
- 238000002156 mixing Methods 0.000 claims abstract description 125
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- 239000007788 liquid Substances 0.000 claims abstract description 34
- 210000004369 blood Anatomy 0.000 claims abstract description 30
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- B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
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Abstract
The invention discloses a microfluidic type thrombus elasticity analysis and detection kit, which solves the technical problems of complex structure, large volume and high production cost of a thrombus elastography instrument in the prior art. The invention comprises a reagent box main body and a reagent box sealing plate which is packaged on the reagent box main body, wherein the reagent box main body is provided with a sample reagent mixing bin, an overflow bin connected with the sample reagent mixing bin and a liquid drop motion detection flow channel connected with the overflow bin, a freeze-dried reagent ball is arranged in the sample reagent mixing bin, and a sample filling opening which is communicated with the sample reagent mixing bin and is used for filling whole blood samples into the sample reagent mixing bin is arranged on the reagent box sealing plate. The microfluidic kit has the advantages of simple structure, scientific and reasonable design and convenient use, can make the structure of the thromboelastography instrument simpler, can effectively reduce the volume of the thromboelastography instrument, reduces the manufacturing cost of the thromboelastography instrument, and can forcefully push the thromboelastography instrument to develop in the miniaturized and integrated directions.
Description
Technical Field
The invention belongs to the technical field of medical equipment, and particularly relates to a microfluidic type thrombus elasticity analysis and detection kit.
Background
Thromboelastography (thromboela-stogram, TEG) is an index reflecting blood coagulation dynamics (including the rate of fibrin formation, the dissolution state and the firmness of coagulation, elasticity), and thus factors affecting thromboelastography are mainly: the aggregation state of erythrocytes, the rigidity of erythrocytes, the speed of blood coagulation, the activity of fibrinolytic system, and the like.
Thromboelastography (thrombelastogram, TEG) is a special figure that is depicted by a thromboelastometer. The main components of the elastometer; automatically adjusting a stainless steel blood cup with constant temperature (37 ℃) and a small stainless steel cylinder and a sensor which can be connected with the cylinder, wherein the small stainless steel cylinder is inserted into the cup. The blood cup is arranged on the reaction tank which can rotate back and forth at an angle of 4 degrees 45', and the middle of the cup wall and the cylinder is used for containing blood. When the blood sample is in liquid state, the back and forth rotation of the cup can not drive the cylinder, the signal reflected to the tracing paper by the sensor is a straight line, when the blood starts to solidify, resistance is generated between the cup and the cylinder due to the adhesiveness of fibrin, the rotation of the cup drives the cylinder to move simultaneously, the resistance is also continuously increased along with the increase of fibrin, the movement of the cup drives the cylinder also changes along with the increase of fibrin, the cylinder moves to cut magnetic force lines to generate current, the current is converted into a digital signal, and the signal is depicted on the tracing paper by the sensor to form a special thromboelastography.
The invention discloses a thromboelastography analyzer for monitoring the whole dynamic processes of platelet aggregation, coagulation, fibrinolysis and the like, which is invented in 1948. The physical properties of a thromboelastography to monitor blood clots are based on the following principles: a custom made cylindrical cup containing blood was rotated at an angle of 4 deg. 45' for 10 seconds per revolution. The movement of the blood sample is monitored by a needle suspended by a spiral wire and immersed in the blood sample. After the fibrin platelet complex adheres the cup and needle together, the rotational force generated by the rotation of the cup can be transferred to the needle in the blood sample. The strength of the fibrin-platelet complex can affect the magnitude of the needle movement so that a strong blood clot can synchronize the needle movement with the cup movement. Thus, the magnitude of the needle movement is directly related to the strength of the formed blood clot. When the blood clot is retracted or dissolved, the coupling of the needle to the blood clot is released and the movement of the cup is no longer transmitted to the needle.
The traditional thromboelastography instrument adopts torsion deformation of suspension wires to drive a metal induction sheet to measure the change of shear stress with time in the dynamic coagulation process. The design is mature in technology and high in reliability, and a certain experience is accumulated after a plurality of years of clinical use; but the measurement accuracy is lower, and the error is larger when multiple channels are detected simultaneously; the suspension wires in the core component belong to sensitive and vulnerable devices, and have higher requirements on design, materials, processing technology, assembly and the like. The traditional thrombelastogram instrument is complex in structure, huge in size and high in cost.
Therefore, the microfluidic type thrombus elasticity analysis and detection kit is designed, and the technical defects of complex instrument, huge volume and high cost of a customer service thrombus elastography instrument are used as technical problems to be solved urgently by the skilled person.
Disclosure of Invention
The invention aims to solve the technical problems that: provides a microfluidic type thrombus elastic force analysis and detection kit for solving at least part of the technical problems.
In order to achieve the above purpose, the technical scheme adopted by the invention is as follows:
The microfluidic type thrombus elastic force analysis detection kit comprises a kit main body and a kit sealing plate which is packaged on the kit main body, wherein a sample reagent mixing bin, an overflow bin connected with the sample reagent mixing bin and a droplet motion detection flow channel connected with the overflow bin are arranged on the kit main body, freeze-drying reagent balls are arranged in the sample reagent mixing bin, and a sample filling port which is communicated with the sample reagent mixing bin and is used for filling whole blood samples into the sample reagent mixing bin is arranged on the kit sealing plate.
Further, a low-speed sealing port communicated with the sample reagent mixing bin is arranged on the reagent box main body, a high-speed conduction flow passage is communicated between the overflow bin and the low-speed sealing port, and the overflow bin is sequentially connected out of the sample reagent mixing bin through the low-speed sealing port and the high-speed conduction flow passage.
Further, two ends of the liquid drop movement detection flow channel are respectively connected with an air ventilation flow channel (1-11), and the air ventilation flow channels (1-11) are communicated with the high-speed conduction flow channel.
Further, the sample reagent mixing bin is communicated with a sample filling port runner, and the sample filling port runner is positioned right below the sample filling port and is communicated with the sample filling port runner.
Further, a sample adding air hole communicated with the sample reagent mixing bin is arranged on the reagent box sealing plate.
Further, the sample reagent mixing bin is communicated with a sample adding air hole runner, and the sample reagent mixing bin is positioned right below the sample adding air hole and is communicated with the sample adding air hole runner.
Further, a sample guiding triangular rib is arranged in the sample reagent mixing bin, and a sample filling port runner and a sample filling air hole runner are symmetrically arranged at two sides of the sample guiding triangular rib.
Further, an overflow flow passage is arranged on the reagent box main body, and two ends of the overflow flow passage are respectively communicated with the overflow bin and the liquid drop movement detection flow passage.
Further, the overflow flow passage is connected to the middle of the liquid drop movement detection flow passage.
Further, the kit main body and the kit sealing plate are formed by injection molding of transparent plastic, and are sealed by ultrasonic welding, and the kit main body packaged with the kit sealing plate is in a sector shape with an arc of 90 degrees.
Compared with the prior art, the invention has the following beneficial effects:
the microfluidic kit has the advantages of simple structure, scientific and reasonable design and convenient use, can make the structure of the thromboelastography instrument simpler, can effectively reduce the volume of the thromboelastography instrument, reduces the manufacturing cost of the thromboelastography instrument, and can forcefully push the thromboelastography instrument to develop in the miniaturized and integrated directions.
Drawings
FIG. 1 is an explosion schematic diagram of a microfluidic thromboelastography detection kit of the present invention.
FIG. 2 is a schematic diagram of the main structure of the kit of the present invention.
FIG. 3 is a schematic diagram showing the appearance of the kit of the present invention.
FIG. 4 is a schematic diagram showing the combined detection states of four kits according to the present invention.
Wherein, the names corresponding to the reference numerals are:
1. A kit body; 2. freeze-drying the reagent balls; 3. a reagent box sealing plate; 4. hole matching; 5. a microfluidic type thrombus elastic force analysis and detection kit 5;3-1, a sample filling port; 3-2, sample adding ventilation holes; 1-1, a sample filling port runner; 1-2, uniformly mixing sample reagents in a bin; 1-3, a sample adding ventilation hole runner; 1-4, sample guiding triangular ribs; 1-5, a low-speed sealing port; 1-6, high-speed conduction flow channels; 1-7, an overflow bin; 1-8, overflow flow channel; 1-9, a liquid drop movement detection flow channel; 1-10, liquid drops; 1-11, a ventilation runner; 1-12, a ventilation channel and a ventilation port.
Detailed Description
The present invention will be described in further detail with reference to the accompanying drawings, in order to make the objects, technical solutions and advantages of the present invention more apparent. It will be apparent that the described embodiments are only some, but not all, embodiments of the invention. All other embodiments, which can be made by those skilled in the art based on the embodiments of the invention without making any inventive effort, are intended to be within the scope of the invention.
1-4, The microfluidic type thrombus elastic analysis and detection kit 5 provided by the invention comprises a kit main body 1 and a kit sealing plate 3 packaged on the kit main body 1, wherein a sample reagent mixing bin 1-2, an overflow bin 1-7 connected with the sample reagent mixing bin 1-2 and a droplet motion detection flow channel 1-9 connected with the overflow bin 1-7 are arranged on the kit main body 1, a freeze-dried reagent ball 2 is arranged in the sample reagent mixing bin 1-2, and a sample filling port 3-1 communicated with the sample reagent mixing bin 1-2 and used for filling whole blood samples into the sample reagent mixing bin 1-2 is arranged on the kit sealing plate 3.
The microfluidic kit has the advantages of simple structure, scientific and reasonable design and convenient use, can make the structure of the thromboelastography instrument simpler, can effectively reduce the volume of the thromboelastography instrument, reduces the manufacturing cost of the thromboelastography instrument, and can forcefully push the thromboelastography instrument to develop in the miniaturized and integrated directions.
Embodiment 2, as shown in fig. 1-4, the microfluidic type thrombus elastic analysis detection kit 5 provided by the invention comprises a kit main body 1 and a kit sealing plate 3 packaged on the kit main body 1, wherein a sample reagent mixing bin 1-2, an overflow bin 1-7 connected with the sample reagent mixing bin 1-2 and a droplet motion detection flow channel 1-9 connected with the overflow bin 1-7 are arranged on the kit main body 1, a freeze-dried reagent ball 2 is arranged in the sample reagent mixing bin 1-2, and a sample filling port 3-1 communicated with the sample reagent mixing bin 1-2 and used for filling whole blood samples into the sample reagent mixing bin 1-2 is arranged on the kit sealing plate 3. The reagent kit main body 1 is provided with a low-speed sealing port 1-5 communicated with a sample reagent mixing bin 1-2, a high-speed conduction flow passage 1-6 is communicated between an overflow bin 1-7 and the low-speed sealing port 1-5, and the overflow bin 1-7 is sequentially connected out of the sample reagent mixing bin 1-2 through the low-speed sealing port 1-5 and the high-speed conduction flow passage 1-6.
In this embodiment 2, on the basis of embodiment 1, a more preferable connection structure between the sample reagent mixing bin 1-2 and the overflow bin 1-7 is provided, specifically: the reagent kit main body 1 is provided with a low-speed sealing port 1-5 communicated with a sample reagent mixing bin 1-2, a high-speed conduction flow passage 1-6 is communicated between an overflow bin 1-7 and the low-speed sealing port 1-5, and the overflow bin 1-7 is sequentially connected out of the sample reagent mixing bin 1-2 through the low-speed sealing port 1-5 and the high-speed conduction flow passage 1-6. The sample reagent mixing bin 1-2 is connected with the overflow bin 1-7 sequentially through the low-speed sealing port 1-5 and the high-speed conduction flow channel 1-6, when the reagent box main body 1 rotates at a low speed, the mixture in the sample reagent mixing bin 1-2 cannot break through the low-speed sealing port 1-5 and enter the high-speed conduction flow channel 1-6 and the overflow bin 1-7, so that the mixture in the sample reagent mixing bin 1-2 can be uniformly mixed under the low-speed rotation, after uniform mixing, the rotation speed of the reagent box main body 1 is improved, the mixture in the sample reagent mixing bin 1-2 can break through the low-speed sealing port 1-5 under the high-speed rotation, and sequentially enters the overflow bin 1-7 through the low-speed sealing port 1-5 and the high-speed conduction flow channel 1-6.
Embodiment 3, as shown in fig. 1-4, the microfluidic type thrombus elastic analysis detection kit 5 provided by the invention comprises a kit main body 1 and a kit sealing plate 3 packaged on the kit main body 1, wherein a sample reagent mixing bin 1-2, an overflow bin 1-7 connected with the sample reagent mixing bin 1-2 and a droplet motion detection flow channel 1-9 connected with the overflow bin 1-7 are arranged on the kit main body 1, a freeze-dried reagent ball 2 is arranged in the sample reagent mixing bin 1-2, and a sample filling port 3-1 communicated with the sample reagent mixing bin 1-2 and used for filling whole blood samples into the sample reagent mixing bin 1-2 is arranged on the kit sealing plate 3. The reagent kit main body 1 is provided with a low-speed sealing port 1-5 communicated with a sample reagent mixing bin 1-2, a high-speed conduction flow passage 1-6 is communicated between an overflow bin 1-7 and the low-speed sealing port 1-5, and the overflow bin 1-7 is sequentially connected out of the sample reagent mixing bin 1-2 through the low-speed sealing port 1-5 and the high-speed conduction flow passage 1-6. Two ends of the liquid drop movement detection flow channel 1-9 are respectively connected with an air ventilation flow channel 1-11, and the air ventilation flow channel 1-11 is communicated with the high-speed conduction flow channel 1-6.
This embodiment 3 gives a more preferable connection structure between the droplet motion detection flow passages 1 to 9 and the high-speed conduction flow passages 1 to 6 on the basis of embodiment 2, specifically: two ends of the liquid drop movement detection flow channel 1-9 are respectively connected with an air ventilation flow channel 1-11, and the air ventilation flow channel 1-11 is communicated with the high-speed conduction flow channel 1-6. The two ends of the designed droplet motion detection flow channel 1-9 are respectively communicated with the high-speed conduction flow channel 1-6 through a ventilation flow channel 1-11, so that the droplets 1-10 formed in the droplet motion detection flow channel 1-9 can run along the droplet motion detection flow channel 1-9 when the reagent kit main body 1 rotates and runs, and the detection is ensured to be carried out smoothly. The structure design is simple, reliable and exquisite, and the movement detection of the liquid drops 1-10 in the liquid drop movement detection flow channels 1-9 is smooth and controllable.
Embodiment 4, as shown in fig. 1-4, the microfluidic type thrombus elastic analysis detection kit 5 provided by the invention comprises a kit main body 1 and a kit sealing plate 3 packaged on the kit main body 1, wherein a sample reagent mixing bin 1-2, an overflow bin 1-7 connected with the sample reagent mixing bin 1-2 and a droplet motion detection flow channel 1-9 connected with the overflow bin 1-7 are arranged on the kit main body 1, a freeze-dried reagent ball 2 is arranged in the sample reagent mixing bin 1-2, and a sample filling port 3-1 communicated with the sample reagent mixing bin 1-2 and used for filling whole blood samples into the sample reagent mixing bin 1-2 is arranged on the kit sealing plate 3. The sample reagent mixing bin 1-2 is communicated with a sample filling port runner 1-1, and the sample filling port runner 1-1 is positioned right below the sample filling port 3-1 and is communicated with the sample filling port runner 1-1.
In this embodiment 4, on the basis of embodiment 1, a more preferable connection structure between the sample reagent mixing chamber 1-2 and the sample filling port 3-1 is provided, specifically: the sample reagent mixing bin 1-2 is communicated with a sample filling port runner 1-1, and the sample filling port runner 1-1 is positioned right below the sample filling port 3-1 and is communicated with the sample filling port runner 1-1. A sample filling port runner 1-1 arranged right below the sample filling port 3-1 is arranged between the sample reagent mixing bin 1-2 and the sample filling port 3-1, and samples can smoothly enter the sample reagent mixing bin 1-2 along the sample filling port runner 1-1 after being filled through the sample filling port 3-1. The structural design is simple, reliable and exquisite, and a sample filled through the sample filling port 3-1 can smoothly enter the sample reagent mixing bin 1-2.
Embodiment 5, as shown in fig. 1-4, the microfluidic type thrombus elastic analysis detection kit 5 provided by the invention comprises a kit main body 1 and a kit sealing plate 3 packaged on the kit main body 1, wherein a sample reagent mixing bin 1-2, an overflow bin 1-7 connected with the sample reagent mixing bin 1-2 and a droplet motion detection flow channel 1-9 connected with the overflow bin 1-7 are arranged on the kit main body 1, a freeze-dried reagent ball 2 is arranged in the sample reagent mixing bin 1-2, and a sample filling port 3-1 communicated with the sample reagent mixing bin 1-2 and used for filling whole blood samples into the sample reagent mixing bin 1-2 is arranged on the kit sealing plate 3. The sample reagent mixing bin 1-2 is communicated with a sample filling port runner 1-1, and the sample filling port runner 1-1 is positioned right below the sample filling port 3-1 and is communicated with the sample filling port runner 1-1. The reagent box sealing plate 3 is provided with a sample adding vent hole 3-2 communicated with the sample reagent mixing bin 1-2.
In this embodiment 5, a more preferable structure of the cartridge sealing plate 3 is given on the basis of embodiment 4, specifically: the reagent box sealing plate 3 is provided with a sample adding vent hole 3-2 communicated with the sample reagent mixing bin 1-2. The sample adding air holes 3-2 are communicated with the outside atmosphere, so that samples can be more conveniently filled into the sample reagent mixing bin 1-2 through the sample filling opening 3-1 and the sample filling opening runner 1-1, the structural design is simple, reliable and exquisite, and the sample adding air holes 3-2 play a role in exhausting when the samples are filled into the sample reagent mixing bin 1-2.
Embodiment 6, as shown in fig. 1-4, the microfluidic type thrombus elastic analysis detection kit 5 provided by the invention comprises a kit main body 1 and a kit sealing plate 3 packaged on the kit main body 1, wherein a sample reagent mixing bin 1-2, an overflow bin 1-7 connected with the sample reagent mixing bin 1-2 and a droplet motion detection flow channel 1-9 connected with the overflow bin 1-7 are arranged on the kit main body 1, a freeze-dried reagent ball 2 is arranged in the sample reagent mixing bin 1-2, and a sample filling port 3-1 communicated with the sample reagent mixing bin 1-2 and used for filling whole blood samples into the sample reagent mixing bin 1-2 is arranged on the kit sealing plate 3. The sample reagent mixing bin 1-2 is communicated with a sample filling port runner 1-1, and the sample filling port runner 1-1 is positioned right below the sample filling port 3-1 and is communicated with the sample filling port runner 1-1. The reagent box sealing plate 3 is provided with a sample adding vent hole 3-2 communicated with the sample reagent mixing bin 1-2. The sample reagent mixing bin 1-2 is communicated with a sample adding air vent runner 1-3, and the sample reagent mixing bin 1-2 is positioned right below the sample adding air vent 3-2 and is communicated with the sample adding air vent runner 1-3.
In this embodiment 6, on the basis of embodiment 5, a more preferable connection structure between the sample adding vent hole 3-2 and the sample reagent mixing bin 1-2 is provided, specifically: the sample reagent mixing bin 1-2 is communicated with a sample adding air vent runner 1-3, and the sample reagent mixing bin 1-2 is positioned right below the sample adding air vent 3-2 and is communicated with the sample adding air vent runner 1-3. The smooth filling of the sample into the sample reagent mixing bin 1-2 is ensured, and meanwhile, the direct contact between the inside of the sample reagent mixing bin 1-2 and the outside atmosphere can be effectively avoided.
Embodiment 7, as shown in fig. 1-4, the microfluidic type thrombus elastic analysis detection kit 5 provided by the invention comprises a kit main body 1 and a kit sealing plate 3 packaged on the kit main body 1, wherein a sample reagent mixing bin 1-2, an overflow bin 1-7 connected with the sample reagent mixing bin 1-2 and a droplet motion detection flow channel 1-9 connected with the overflow bin 1-7 are arranged on the kit main body 1, a freeze-dried reagent ball 2 is arranged in the sample reagent mixing bin 1-2, and a sample filling port 3-1 communicated with the sample reagent mixing bin 1-2 and used for filling whole blood samples into the sample reagent mixing bin 1-2 is arranged on the kit sealing plate 3. The sample reagent mixing bin 1-2 is communicated with a sample filling port runner 1-1, and the sample filling port runner 1-1 is positioned right below the sample filling port 3-1 and is communicated with the sample filling port runner 1-1. The reagent box sealing plate 3 is provided with a sample adding vent hole 3-2 communicated with the sample reagent mixing bin 1-2. The sample reagent mixing bin 1-2 is communicated with a sample adding air vent runner 1-3, and the sample reagent mixing bin 1-2 is positioned right below the sample adding air vent 3-2 and is communicated with the sample adding air vent runner 1-3. The sample reagent mixing bin 1-2 is internally provided with a sample guiding triangular rib 1-4, and the sample filling port runner 1-1 and the sample filling vent runner 1-3 are symmetrically arranged at two sides of the sample guiding triangular rib 1-4.
In this embodiment 7, on the basis of embodiment 6, a more preferable structure of the inside of the sample reagent mixing bin 1-2 is given, specifically: the sample reagent mixing bin 1-2 is internally provided with a sample guiding triangular rib 1-4, and the sample filling port runner 1-1 and the sample filling vent runner 1-3 are symmetrically arranged at two sides of the sample guiding triangular rib 1-4. Through the separation effect of the sample guiding triangular ribs 1-4, the normal ventilation effect of the sample adding ventilation hole flow channel 1-3 can be ensured, and the sample just added into the sample reagent mixing bin 1-2 is prevented from entering the sample adding ventilation hole flow channel 1-3 to form ventilation blockage.
Embodiment 8, as shown in fig. 1-4, the microfluidic type thrombus elastic analysis detection kit 5 provided by the invention comprises a kit main body 1 and a kit sealing plate 3 packaged on the kit main body 1, wherein a sample reagent mixing bin 1-2, an overflow bin 1-7 connected with the sample reagent mixing bin 1-2 and a droplet motion detection flow channel 1-9 connected with the overflow bin 1-7 are arranged on the kit main body 1, a freeze-dried reagent ball 2 is arranged in the sample reagent mixing bin 1-2, and a sample filling port 3-1 communicated with the sample reagent mixing bin 1-2 and used for filling whole blood samples into the sample reagent mixing bin 1-2 is arranged on the kit sealing plate 3. The reagent box main body 1 is provided with an overflow flow passage 1-8, and two ends of the overflow flow passage 1-8 are respectively communicated with an overflow bin 1-7 and a liquid drop movement detection flow passage 1-9.
In this embodiment 8, on the basis of embodiment 1, a more preferable connection structure between the overflow tanks 1 to 7 and the droplet motion detection flow paths 1 to 9 is provided, specifically: the reagent box main body 1 is provided with an overflow flow passage 1-8, and two ends of the overflow flow passage 1-8 are respectively communicated with an overflow bin 1-7 and a liquid drop movement detection flow passage 1-9. The mixture entering the overflow bin 1-7 from the sample reagent mixing bin 1-2 can enter the liquid drop motion detection flow channel 1-9 along the overflow flow channel 1-8 to form a section of liquid drop 1-10, so that the detection is ensured to be carried out smoothly. The structural design is simple, reliable and exquisite.
Embodiment 9, as shown in fig. 1-4, the microfluidic type thrombus elastic analysis detection kit 5 provided by the invention comprises a kit main body 1 and a kit sealing plate 3 packaged on the kit main body 1, wherein a sample reagent mixing bin 1-2, an overflow bin 1-7 connected with the sample reagent mixing bin 1-2 and a droplet motion detection flow channel 1-9 connected with the overflow bin 1-7 are arranged on the kit main body 1, a freeze-dried reagent ball 2 is arranged in the sample reagent mixing bin 1-2, and a sample filling port 3-1 communicated with the sample reagent mixing bin 1-2 and used for filling whole blood samples into the sample reagent mixing bin 1-2 is arranged on the kit sealing plate 3. The reagent box main body 1 is provided with an overflow flow passage 1-8, and two ends of the overflow flow passage 1-8 are respectively communicated with an overflow bin 1-7 and a liquid drop movement detection flow passage 1-9. The overflow channels 1-8 are connected to the middle of the liquid drop movement detection channels 1-9.
This embodiment 9 gives a more preferable connection structure between the overflow channels 1 to 8 and the droplet motion detection channels 1 to 9 on the basis of embodiment 8, specifically: the overflow channels 1-8 are connected to the middle of the liquid drop movement detection channels 1-9. By the design, the initial position of the liquid drop 1-10 formed in the liquid drop movement detection flow channel 1-9 from the overflow flow channel 1-8 can be effectively ensured to be positioned in the middle of the liquid drop movement detection flow channel 1-9, and the liquid drop is prevented from being thrown out from the liquid drop movement detection flow channel 1-9 in the rotation detection process. The structural design is simple, reliable and exquisite.
Embodiment 10, as shown in fig. 1-4, the microfluidic type thrombus elastic analysis detection kit 5 provided by the invention comprises a kit main body 1 and a kit sealing plate 3 packaged on the kit main body 1, wherein a sample reagent mixing bin 1-2, an overflow bin 1-7 connected with the sample reagent mixing bin 1-2 and a droplet motion detection flow channel 1-9 connected with the overflow bin 1-7 are arranged on the kit main body 1, a freeze-dried reagent ball 2 is arranged in the sample reagent mixing bin 1-2, and a sample filling port 3-1 communicated with the sample reagent mixing bin 1-2 and used for filling whole blood samples into the sample reagent mixing bin 1-2 is arranged on the kit sealing plate 3. The reagent box main body 1 and the reagent box sealing plate 3 are formed by injection molding of transparent plastic, and the reagent box main body 1 which is sealed with the reagent box sealing plate 3 by ultrasonic welding is in a sector shape with an arc of 90 degrees.
In this embodiment 10, on the basis of embodiment 1, a more preferable structure and a connection structure between the cartridge body 1 and the cartridge sealing plate 3 are given, specifically: the reagent box main body 1 and the reagent box sealing plate 3 are formed by injection molding of transparent plastic, and the reagent box main body 1 which is sealed with the reagent box sealing plate 3 by ultrasonic welding is in a sector shape with an arc of 90 degrees. The two are sealed by ultrasonic welding, so that the sealing performance is good, the transparent plastic injection molding is adopted, the manufacturing is simple and convenient, and the sealing device is suitable for industrial mass production. The main body 1 and the sealing plate 3 are designed to be a sector with an arc of 90 deg., so that four cartridges can be detected at a time, as shown in fig. 4. Of course, other degrees of fan shape may be designed, such as 45 °, 60 °, 120 °, 180 °, etc. Thus, the method can be suitable for simultaneous detection of kits with different numbers. The main body 1 and the sealing plate 3 are also provided with a fitting hole 4 for fitting to a thromboelastography machine.
The invention mainly comprises a kit main body 1, freeze-dried reagent balls 2 and a kit sealing plate 3. The kit main body 1 and the kit cover plate 3 are made of transparent materials by injection molding, such as PMMA, freeze-dried reagent balls 2 of different items are put into a sample reagent mixing bin 1-2 in the kit main body 1 during production, then the kit cover plate 3 is covered, and the sealing of a flow channel and a bin body in the kit main body 1 is realized by means of ultrasonic welding or bonding and the like.
When the full blood sample filling device is used, a quantitative full blood sample is filled through the sample filling port 3-1, the sample filling air holes 3-2 are used for communicating with the atmosphere, the full blood sample is conveniently filled, then the full blood sample is put into the reagent position of the detection instrument, the four reagent boxes or the reagent boxes and the balancing weight reach the equilibrium state shown in the figure 4, and the detection instrument controls the reagent boxes according to a set program, so that the states and the state duration time of rotation, rest, speed change, forward and reverse rotation, heating and the like are controlled. Finally, signal detection is realized through a detection component, a detection result is obtained through analysis and treatment, and finally, the kit is taken out and put into a medical waste garbage can.
The whole blood sample flows as follows: firstly, the kit rotates at a low speed, under the action of centrifugal force, the whole blood sample in the sample reagent mixing bin 1-2 is mixed and dissolved with the freeze-dried reagent balls 2, and then the kit rotates in a direction-changing way at a low speed, so that the whole blood sample and the reagent are fully mixed. Due to the low rotation speed, the mixed liquid of the whole blood sample and the freeze-dried reagent ball 2 can not break through the low-speed sealing ports 1-5, and the gas is sealed inside the kit. In the second step, the high-speed rotating kit breaks through the low-speed sealing port 1-5 due to the increase of the centrifugal force applied to the mixed liquid, enters the high-speed conducting channel 1-6, fills the overflow bin 1-7, overflows a large amount of mixed liquid through the overflow channel 1-8, and finally flows into the liquid drop motion detection channel 1-9 to form a segment of liquid drop 1-10 as shown in fig. 2. At this time, due to the action of centrifugal force, the reagent mixed solution in the kit is far away from the sample reagent mixing bin 1-2, so that the reagent mixed solution is prevented from leaking out of the sample adding air holes 3-2 through the sample adding air hole flow channels 1-3. Thirdly, the reagent box rotates positively, the reagent box is slowly accelerated to a very high speed, then the reagent box is suddenly stopped, the inertia of the liquid drops 1-10 is utilized to move, and then the reagent box rotates slowly to detect the positions of the liquid drops. Fourth, the reagent box is reversed, the reagent box is slowly accelerated to a very high speed, then suddenly stopped, the inertia of the liquid drops 1-10 is utilized to move, and then the reagent box is slowly rotated to detect the positions of the liquid drops. Fifth, repeating the third and fourth steps a plurality of times. And finally, obtaining a plurality of position data of the liquid drops which move back and forth, making an image according to the data, and obtaining a result through analysis and calculation.
Microfluidic technology refers to technology for manipulating micro-fluids using micro-channels and various microstructures, and is an emerging interdisciplinary discipline involving chemical, fluid physics, microelectronics, new materials, biology, and biomedical engineering. Because of the characteristics of miniaturization, integration and the like, the microfluidic device is commonly called a microfluidic chip or a lab-on-a-chip, and a small microfluidic chip is equivalent to integrating basic operations of sample preparation, reaction, separation, detection and the like in biological, chemical and medical analysis processes together, and is matched with an instrument to automatically complete the whole analysis process. The microfluidic chip generally has the advantages of low sample consumption, high detection speed, simple and convenient operation, multifunctional integration, small volume, portability and the like, so that the microfluidic chip has great potential of simplifying diagnosis flow, reducing medical cost and improving medical efficiency.
Finally, it should be noted that: the above embodiments are merely preferred embodiments of the present invention for illustrating the technical solution of the present invention, but not limiting the scope of the present invention; although the invention has been described in detail with reference to the foregoing embodiments, it will be understood by those of ordinary skill in the art that: the technical scheme described in the foregoing embodiments can be modified or some or all of the technical features thereof can be replaced by equivalents; such modifications and substitutions do not depart from the spirit of the corresponding technical solutions; that is, even though the main design concept and spirit of the present invention is modified or finished in an insubstantial manner, the technical problem solved by the present invention is still consistent with the present invention, and all the technical problems are included in the protection scope of the present invention; in addition, the technical scheme of the invention is directly or indirectly applied to other related technical fields, and the technical scheme is included in the scope of the invention.
Claims (8)
1. The microfluidic type thrombus elastic analysis detection kit is characterized by comprising a kit main body (1) and a kit sealing plate (3) which is packaged on the kit main body (1), wherein a sample reagent mixing bin (1-2), an overflow bin (1-7) which is connected with the sample reagent mixing bin (1-2) and a droplet motion detection flow channel (1-9) which is connected with the overflow bin (1-7) are arranged on the kit main body (1), a freeze-dried reagent ball (2) is arranged in the sample reagent mixing bin (1-2), and a sample injection port (3-1) which is communicated with the sample reagent mixing bin (1-2) and is used for injecting a whole blood sample into the sample reagent mixing bin (1-2) is arranged on the kit sealing plate (3); the reagent kit comprises a reagent kit main body (1), a sample reagent mixing bin (1-2), a low-speed sealing port (1-5) communicated with the sample reagent mixing bin (1-2), a high-speed conduction flow channel (1-6) communicated between an overflow bin (1-7) and the low-speed sealing port (1-5), and the overflow bin (1-7) sequentially connected with the sample reagent mixing bin (1-2) through the low-speed sealing port (1-5) and the high-speed conduction flow channel (1-6);
The reagent box main body (1) is provided with an overflow flow passage (1-8), and two ends of the overflow flow passage (1-8) are respectively communicated with the overflow bin (1-7) and the liquid drop movement detection flow passage (1-9).
2. The microfluidic type thrombus elasticity analysis and detection kit according to claim 1, wherein two ends of the droplet motion detection flow channel (1-9) are respectively connected with an air permeable flow channel (1-11), and the air permeable flow channel (1-11) is communicated with the high-speed conduction flow channel (1-6).
3. The microfluidic type thrombus elastic analysis and detection kit according to claim 1, wherein the sample reagent mixing bin (1-2) is communicated with a sample filling port runner (1-1), and the sample filling port runner (1-1) is positioned right below the sample filling port (3-1) and is communicated with the sample filling port runner.
4. A microfluidic type thrombus elastic analysis and detection kit according to claim 3, wherein the kit sealing plate (3) is provided with a sample feeding vent hole (3-2) communicated with the sample reagent mixing bin (1-2).
5. The microfluidic type thrombus elastic force analysis and detection kit according to claim 4, wherein the sample reagent mixing bin (1-2) is communicated with the sample adding vent hole runner (1-3), and the sample reagent mixing bin (1-2) is positioned under the sample adding vent hole (3-2) and is communicated with the sample adding vent hole runner (1-3).
6. The microfluidic type thrombus elastic force analysis and detection kit according to claim 5, wherein a sample guiding triangular rib (1-4) is arranged in the sample reagent mixing bin (1-2), and the sample filling port runner (1-1) and the sample filling vent runner (1-3) are symmetrically distributed on two sides of the sample guiding triangular rib (1-4).
7. The microfluidic thromboelastography assay kit according to claim 1, wherein the overflow channel (1-8) is connected to the middle of the droplet motion detection channel (1-9).
8. The microfluidic type thrombus elastic analysis and detection kit according to claim 1, wherein the kit main body (1) and the kit sealing plate (3) are formed by injection molding of transparent plastics, and the kit main body (1) with the kit sealing plate (3) sealed by ultrasonic welding is in a sector shape with an arc of 90 degrees.
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| CN117368454B (en) * | 2023-12-08 | 2024-04-23 | 普迈德（北京）科技有限公司 | Thromboelastography rapid activated blood coagulation reagent card, preparation method and application |
| CN117783570B (en) * | 2024-02-27 | 2024-05-31 | 烟台艾德康生物科技有限公司 | Detection mechanism and full-automatic thromboelastography appearance |
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