CN114532449A - Feed for increasing conjugated linoleic acid content in milk, feeding method and feed additive - Google Patents
Feed for increasing conjugated linoleic acid content in milk, feeding method and feed additive Download PDFInfo
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- CN114532449A CN114532449A CN202011333790.7A CN202011333790A CN114532449A CN 114532449 A CN114532449 A CN 114532449A CN 202011333790 A CN202011333790 A CN 202011333790A CN 114532449 A CN114532449 A CN 114532449A
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- oil
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- 235000013336 milk Nutrition 0.000 title claims abstract description 78
- 239000008267 milk Substances 0.000 title claims abstract description 78
- 210000004080 milk Anatomy 0.000 title claims abstract description 78
- OYHQOLUKZRVURQ-IXWMQOLASA-N linoleic acid Natural products CCCCC\C=C/C\C=C\CCCCCCCC(O)=O OYHQOLUKZRVURQ-IXWMQOLASA-N 0.000 title claims abstract description 41
- 238000000034 method Methods 0.000 title claims abstract description 37
- 229940108924 conjugated linoleic acid Drugs 0.000 title claims abstract description 34
- JBYXPOFIGCOSSB-GOJKSUSPSA-N 9-cis,11-trans-octadecadienoic acid Chemical compound CCCCCC\C=C\C=C/CCCCCCCC(O)=O JBYXPOFIGCOSSB-GOJKSUSPSA-N 0.000 title claims abstract description 33
- 239000003674 animal food additive Substances 0.000 title claims abstract description 33
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- 235000013365 dairy product Nutrition 0.000 claims abstract description 46
- 235000021388 linseed oil Nutrition 0.000 claims abstract description 42
- 239000000944 linseed oil Substances 0.000 claims abstract description 42
- 235000004426 flaxseed Nutrition 0.000 claims abstract description 40
- 244000025254 Cannabis sativa Species 0.000 claims abstract description 37
- 235000012766 Cannabis sativa ssp. sativa var. sativa Nutrition 0.000 claims abstract description 37
- 235000012765 Cannabis sativa ssp. sativa var. spontanea Nutrition 0.000 claims abstract description 37
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- 235000005607 chanvre indien Nutrition 0.000 claims abstract description 37
- 239000011487 hemp Substances 0.000 claims abstract description 37
- MJYQFWSXKFLTAY-OVEQLNGDSA-N (2r,3r)-2,3-bis[(4-hydroxy-3-methoxyphenyl)methyl]butane-1,4-diol;(2r,3r,4s,5s,6r)-6-(hydroxymethyl)oxane-2,3,4,5-tetrol Chemical compound OC[C@H]1O[C@@H](O)[C@H](O)[C@@H](O)[C@@H]1O.C1=C(O)C(OC)=CC(C[C@@H](CO)[C@H](CO)CC=2C=C(OC)C(O)=CC=2)=C1 MJYQFWSXKFLTAY-OVEQLNGDSA-N 0.000 claims abstract description 33
- 241000283690 Bos taurus Species 0.000 claims description 63
- 235000016709 nutrition Nutrition 0.000 claims description 51
- GVJHHUAWPYXKBD-UHFFFAOYSA-N (±)-α-Tocopherol Chemical compound OC1=C(C)C(C)=C2OC(CCCC(C)CCCC(C)CCCC(C)C)(C)CCC2=C1C GVJHHUAWPYXKBD-UHFFFAOYSA-N 0.000 claims description 22
- 239000000843 powder Substances 0.000 claims description 21
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- 239000004367 Lipase Substances 0.000 claims description 11
- BUGBHKTXTAQXES-UHFFFAOYSA-N Selenium Chemical compound [Se] BUGBHKTXTAQXES-UHFFFAOYSA-N 0.000 claims description 11
- 229930003427 Vitamin E Natural products 0.000 claims description 11
- WIGCFUFOHFEKBI-UHFFFAOYSA-N gamma-tocopherol Natural products CC(C)CCCC(C)CCCC(C)CCCC1CCC2C(C)C(O)C(C)C(C)C2O1 WIGCFUFOHFEKBI-UHFFFAOYSA-N 0.000 claims description 11
- 235000019421 lipase Nutrition 0.000 claims description 11
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- OYHQOLUKZRVURQ-HZJYTTRNSA-N Linoleic acid Chemical compound CCCCC\C=C/C\C=C/CCCCCCCC(O)=O OYHQOLUKZRVURQ-HZJYTTRNSA-N 0.000 claims description 8
- 238000000576 coating method Methods 0.000 claims description 8
- 235000020778 linoleic acid Nutrition 0.000 claims description 8
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- CONKBQPVFMXDOV-QHCPKHFHSA-N 6-[(5S)-5-[[4-[2-(2,3-dihydro-1H-inden-2-ylamino)pyrimidin-5-yl]piperazin-1-yl]methyl]-2-oxo-1,3-oxazolidin-3-yl]-3H-1,3-benzoxazol-2-one Chemical compound C1C(CC2=CC=CC=C12)NC1=NC=C(C=N1)N1CCN(CC1)C[C@H]1CN(C(O1)=O)C1=CC2=C(NC(O2)=O)C=C1 CONKBQPVFMXDOV-QHCPKHFHSA-N 0.000 description 4
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- WTFUTSCZYYCBAY-SXBRIOAWSA-N 6-[(E)-C-[[4-[2-(2,3-dihydro-1H-inden-2-ylamino)pyrimidin-5-yl]piperazin-1-yl]methyl]-N-hydroxycarbonimidoyl]-3H-1,3-benzoxazol-2-one Chemical compound C1C(CC2=CC=CC=C12)NC1=NC=C(C=N1)N1CCN(CC1)C/C(=N/O)/C1=CC2=C(NC(O2)=O)C=C1 WTFUTSCZYYCBAY-SXBRIOAWSA-N 0.000 description 3
- DFGKGUXTPFWHIX-UHFFFAOYSA-N 6-[2-[4-[2-(2,3-dihydro-1H-inden-2-ylamino)pyrimidin-5-yl]piperazin-1-yl]acetyl]-3H-1,3-benzoxazol-2-one Chemical compound C1C(CC2=CC=CC=C12)NC1=NC=C(C=N1)N1CCN(CC1)CC(=O)C1=CC2=C(NC(O2)=O)C=C1 DFGKGUXTPFWHIX-UHFFFAOYSA-N 0.000 description 3
- 102000015303 Fatty Acid Synthases Human genes 0.000 description 3
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- UFTFJSFQGQCHQW-UHFFFAOYSA-N triformin Chemical compound O=COCC(OC=O)COC=O UFTFJSFQGQCHQW-UHFFFAOYSA-N 0.000 description 3
- OHVLMTFVQDZYHP-UHFFFAOYSA-N 1-(2,4,6,7-tetrahydrotriazolo[4,5-c]pyridin-5-yl)-2-[4-[2-[[3-(trifluoromethoxy)phenyl]methylamino]pyrimidin-5-yl]piperazin-1-yl]ethanone Chemical compound N1N=NC=2CN(CCC=21)C(CN1CCN(CC1)C=1C=NC(=NC=1)NCC1=CC(=CC=C1)OC(F)(F)F)=O OHVLMTFVQDZYHP-UHFFFAOYSA-N 0.000 description 2
- HMUNWXXNJPVALC-UHFFFAOYSA-N 1-[4-[2-(2,3-dihydro-1H-inden-2-ylamino)pyrimidin-5-yl]piperazin-1-yl]-2-(2,4,6,7-tetrahydrotriazolo[4,5-c]pyridin-5-yl)ethanone Chemical compound C1C(CC2=CC=CC=C12)NC1=NC=C(C=N1)N1CCN(CC1)C(CN1CC2=C(CC1)NN=N2)=O HMUNWXXNJPVALC-UHFFFAOYSA-N 0.000 description 2
- VZSRBBMJRBPUNF-UHFFFAOYSA-N 2-(2,3-dihydro-1H-inden-2-ylamino)-N-[3-oxo-3-(2,4,6,7-tetrahydrotriazolo[4,5-c]pyridin-5-yl)propyl]pyrimidine-5-carboxamide Chemical compound C1C(CC2=CC=CC=C12)NC1=NC=C(C=N1)C(=O)NCCC(N1CC2=C(CC1)NN=N2)=O VZSRBBMJRBPUNF-UHFFFAOYSA-N 0.000 description 2
- LDXJRKWFNNFDSA-UHFFFAOYSA-N 2-(2,4,6,7-tetrahydrotriazolo[4,5-c]pyridin-5-yl)-1-[4-[2-[[3-(trifluoromethoxy)phenyl]methylamino]pyrimidin-5-yl]piperazin-1-yl]ethanone Chemical compound C1CN(CC2=NNN=C21)CC(=O)N3CCN(CC3)C4=CN=C(N=C4)NCC5=CC(=CC=C5)OC(F)(F)F LDXJRKWFNNFDSA-UHFFFAOYSA-N 0.000 description 2
- WZFUQSJFWNHZHM-UHFFFAOYSA-N 2-[4-[2-(2,3-dihydro-1H-inden-2-ylamino)pyrimidin-5-yl]piperazin-1-yl]-1-(2,4,6,7-tetrahydrotriazolo[4,5-c]pyridin-5-yl)ethanone Chemical compound C1C(CC2=CC=CC=C12)NC1=NC=C(C=N1)N1CCN(CC1)CC(=O)N1CC2=C(CC1)NN=N2 WZFUQSJFWNHZHM-UHFFFAOYSA-N 0.000 description 2
- 102000006587 Glutathione peroxidase Human genes 0.000 description 2
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- 102000014171 Milk Proteins Human genes 0.000 description 2
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- NIPNSKYNPDTRPC-UHFFFAOYSA-N N-[2-oxo-2-(2,4,6,7-tetrahydrotriazolo[4,5-c]pyridin-5-yl)ethyl]-2-[[3-(trifluoromethoxy)phenyl]methylamino]pyrimidine-5-carboxamide Chemical compound O=C(CNC(=O)C=1C=NC(=NC=1)NCC1=CC(=CC=C1)OC(F)(F)F)N1CC2=C(CC1)NN=N2 NIPNSKYNPDTRPC-UHFFFAOYSA-N 0.000 description 2
- AFCARXCZXQIEQB-UHFFFAOYSA-N N-[3-oxo-3-(2,4,6,7-tetrahydrotriazolo[4,5-c]pyridin-5-yl)propyl]-2-[[3-(trifluoromethoxy)phenyl]methylamino]pyrimidine-5-carboxamide Chemical compound O=C(CCNC(=O)C=1C=NC(=NC=1)NCC1=CC(=CC=C1)OC(F)(F)F)N1CC2=C(CC1)NN=N2 AFCARXCZXQIEQB-UHFFFAOYSA-N 0.000 description 2
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- YLZOPXRUQYQQID-UHFFFAOYSA-N 3-(2,4,6,7-tetrahydrotriazolo[4,5-c]pyridin-5-yl)-1-[4-[2-[[3-(trifluoromethoxy)phenyl]methylamino]pyrimidin-5-yl]piperazin-1-yl]propan-1-one Chemical compound N1N=NC=2CN(CCC=21)CCC(=O)N1CCN(CC1)C=1C=NC(=NC=1)NCC1=CC(=CC=C1)OC(F)(F)F YLZOPXRUQYQQID-UHFFFAOYSA-N 0.000 description 1
- DEXFNLNNUZKHNO-UHFFFAOYSA-N 6-[3-[4-[2-(2,3-dihydro-1H-inden-2-ylamino)pyrimidin-5-yl]piperidin-1-yl]-3-oxopropyl]-3H-1,3-benzoxazol-2-one Chemical compound C1C(CC2=CC=CC=C12)NC1=NC=C(C=N1)C1CCN(CC1)C(CCC1=CC2=C(NC(O2)=O)C=C1)=O DEXFNLNNUZKHNO-UHFFFAOYSA-N 0.000 description 1
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Classifications
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K50/00—Feeding-stuffs specially adapted for particular animals
- A23K50/10—Feeding-stuffs specially adapted for particular animals for ruminants
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K67/00—Rearing or breeding animals, not otherwise provided for; New or modified breeds of animals
- A01K67/02—Breeding vertebrates
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/30—Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K20/00—Accessory food factors for animal feeding-stuffs
- A23K20/10—Organic substances
- A23K20/158—Fatty acids; Fats; Products containing oils or fats
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K20/00—Accessory food factors for animal feeding-stuffs
- A23K20/10—Organic substances
- A23K20/174—Vitamins
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K20/00—Accessory food factors for animal feeding-stuffs
- A23K20/10—Organic substances
- A23K20/189—Enzymes
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K20/00—Accessory food factors for animal feeding-stuffs
- A23K20/20—Inorganic substances, e.g. oligoelements
- A23K20/30—Oligoelements
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
- Y02P60/00—Technologies relating to agriculture, livestock or agroalimentary industries
- Y02P60/80—Food processing, e.g. use of renewable energies or variable speed drives in handling, conveying or stacking
- Y02P60/87—Re-use of by-products of food processing for fodder production
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Polymers & Plastics (AREA)
- Animal Husbandry (AREA)
- Zoology (AREA)
- Engineering & Computer Science (AREA)
- Food Science & Technology (AREA)
- Environmental Sciences (AREA)
- Animal Behavior & Ethology (AREA)
- Biodiversity & Conservation Biology (AREA)
- Birds (AREA)
- Health & Medical Sciences (AREA)
- Biotechnology (AREA)
- Botany (AREA)
- Molecular Biology (AREA)
- Mycology (AREA)
- Physiology (AREA)
- Inorganic Chemistry (AREA)
- Feed For Specific Animals (AREA)
- Fodder In General (AREA)
Abstract
The invention provides a feed for improving the content of conjugated linoleic acid in milk, a feeding method and a feed additive. The feed of the invention is formed by adding one or more of the following vegetable oil and vegetable oil seeds into the basal ration of the dairy cow: linseed oil, grape seed oil; vegetable oil seeds: the feed comprises flaxseed, oil sunflower seed kernels and hemp seeds, wherein the addition amount of the flaxseed oil accounts for 2.0-5.5% of the total weight of the daily ration dry matter, the addition amount of the grape seed oil accounts for 0.5-5.5% of the total weight of the daily ration dry matter, the addition amount of the flaxseed accounts for 2-10% of the total weight of the daily ration dry matter, the addition amount of the oil sunflower seed kernels accounts for 0.25-2.5% of the total weight of the daily ration dry matter, and the addition amount of the hemp seeds accounts for 1-5% of the total weight of the daily ration dry matter. The feed of the invention can promote the anabolism of milk CLA, improve the content of the raw CLA in the raw milk, and reduce the influence on the health and the production performance of the dairy cow organism.
Description
Technical Field
The invention relates to the field of milk production, and particularly relates to a feed for increasing the content of conjugated linoleic acid in milk, a feeding method and a feed additive.
Background
Conjugated Linoleic Acid (CLA) refers to a mixture of a series of octadecadienoic acid isomers with different positions and spatial configurations containing conjugated double bonds. The conjugated linoleic acid has various important physiological functions and biological activities for promoting human health, comprises the functions of resisting atherosclerosis, tumors, oxidation, inhibiting fat accumulation, reducing body fat, preventing diabetes, regulating immunity and the like, and has positive effects on human health in many aspects. The natural conjugated linoleic acid with biological activity is mainly present in milk, meat and other products of ruminants such as cows, sheep and the like, but the natural content is not high.
One way of producing conjugated linoleic acid is the incomplete microbial hydrogenation of unsaturated fatty acids (PUFAs) in the diet in the rumen of ruminants. The CLA or linoleic acid-rich oil is added into the daily ration of the milk cow, so that the substrate amount of the synthesized CLA can be increased, and the CLA content in the milk is increased.
Disclosure of Invention
Technical problem to be solved
The inventor carries out deep research on the formula of daily ration of the dairy cow and improves the types, processing modes and addition of the feed, thereby increasing the CLA content in the milk through exogenous feed nutrition fortification and reducing the influence on the body health and the production performance of the dairy cow. Therefore, the invention aims to provide the feed for increasing the content of the conjugated linoleic acid in the milk, and the content of the raw CLA in the raw milk is increased by strengthening the nutrition of fat and trace elements in daily ration.
The invention also aims to provide a feeding method for increasing the content of conjugated linoleic acid in milk.
Another object of the present invention is to provide a feed additive capable of increasing the conjugated linoleic acid content in milk.
Technical scheme for solving problems
In order to achieve the above object, one aspect of the present invention provides a feed for increasing the content of conjugated linoleic acid in milk, which is prepared by adding one or more selected from the following vegetable oils and vegetable oil seeds to a basal diet of a cow,
vegetable oil: linseed oil, grape seed oil;
vegetable oil seeds: flaxseed, oil sunflower seed kernel, hemp seed,
the addition amount of the linseed oil accounts for 2.0-5.5% of the total weight of the dry matters of the daily ration, the addition amount of the grape seed oil accounts for 0.5-5.5% of the total weight of the dry matters of the daily ration, the addition amount of the linseed oil accounts for 2-10% of the total weight of the dry matters of the daily ration, the addition amount of the oil sunflower seed kernel accounts for 0.25-2.5% of the total weight of the dry matters of the daily ration, and the addition amount of the hemp seed accounts for 1-5% of the total weight of the dry matters of the daily ration.
In one example, the feed of the present invention is formed by adding two or three selected from the group consisting of the vegetable oil and the vegetable oil seed to the dairy cow basal diet, preferably by adding the linseed oil and the grape seed oil to the dairy cow basal diet, and more preferably by adding the grape seed oil, the linseed oil, and the oil sunflower seed kernel to the dairy cow basal diet.
In one example, the feed of the present invention is comprised of two or three of the vegetable oil seeds added to the dairy cow basal ration. For example, the dairy cow basic ration may be added with the flaxseed and the oil sunflower seed kernel, the dairy cow basic ration may be added with the flaxseed and the hemp seed, the dairy cow basic ration may be added with the oil sunflower seed kernel and the hemp seed, and the dairy cow basic ration may be added with the flaxseed, the oil sunflower seed kernel and the hemp seed.
According to the invention, a nutritional functional bag can be further added into the feed, wherein the nutritional functional bag comprises 15-50 parts by weight of lipase, 0-20 parts by weight of vitamin E and 0-75 parts by weight of organic selenium, and the addition amount of the nutritional functional bag accounts for 0.01-0.3% of the total weight of dry matters of the daily ration.
In the present invention, the vegetable oil is preferably microcapsule powder (i.e., linseed oil microcapsule powder and grape seed oil microcapsule powder) formed by coating processing, wherein the content of fat in the microcapsule powder is 55% or more by weight, and linoleic acid accounts for 60% or more of the total fatty acids.
In the present invention, the dairy cow basal ration may comprise corn silage, alfalfa, oat grass, flaked corn, corn meal, sugar beet particles, soybean meal, soybean hulls, whole cotton seeds, and concentrates.
The invention also provides a feeding method for improving the content of conjugated linoleic acid in milk, which is used for feeding the feed for the cows in the lactation period.
Another aspect of the present invention provides a feed additive comprising one or more selected from the group consisting of vegetable oils and vegetable oil seeds,
vegetable oil: linseed oil, grape seed oil;
vegetable oil seeds: flaxseed, oil sunflower seed kernel, hemp seed,
the addition amount of the linseed oil in the daily ration accounts for 2.0-5.5% of the total weight of the dry matters of the daily ration, the addition amount of the grape seed oil in the daily ration accounts for 0.5-5.5% of the total weight of the dry matters of the daily ration, the addition amount of the linseed oil in the daily ration accounts for 2-10% of the total weight of the dry matters of the daily ration, the addition amount of the oil sunflower seed kernel in the daily ration accounts for 0.25-2.5% of the total weight of the dry matters of the daily ration, and the addition amount of the hemp seed in the daily ration accounts for 1-5% of the total weight of the dry matters of the daily ration.
The feed additive of the invention can further comprise a nutritional function package, wherein the nutritional function package comprises 15-50 parts by weight of lipase, 0-20 parts by weight of vitamin E and 0-75 parts by weight of organic selenium,
the addition amount of the nutritional functional bag in the daily ration accounts for 0.01-0.3% of the total weight of the dry matters of the daily ration.
In the feed additive of the present invention, the vegetable oil is microcapsule powder (i.e., linseed oil microcapsule powder and grape seed oil microcapsule powder) formed by coating processing, and the content of fat in the microcapsule powder is 55% or more and linoleic acid accounts for 60% or more of total fatty acids on a weight basis.
Effects of the invention
According to the feed provided by the invention, fat and trace element nutrition in daily ration are enhanced, so that anabolism of milk CLA can be promoted, the content of raw CLA in raw milk is increased, and the influence on the body health and production performance of dairy cows can be reduced.
In addition, the feed additive provided by the invention can be used for remarkably improving the CLA content of milk through exogenous nutrition addition regulation and control and metabolic conversion of a milk cow organism, and compared with common milk, the CLA content can even reach more than 50mg/100ml of milk.
In addition, the feeding method can obtain the milk with high CLA content, and compared with the method of directly adding CLA into milk and milk products, the feeding method has better absorptivity and functionality, and is simpler.
Detailed Description
The present invention is not limited to these embodiments, and can be modified as appropriate within a range not departing from the gist of the present invention.
< feed >
One embodiment of the invention provides a feed for increasing the content of conjugated linoleic acid in milk, which is formed by adding one or more selected from the following vegetable oil and vegetable oil seeds into the basal ration of the milk cow,
vegetable oil: linseed oil, grape seed oil;
vegetable oil seeds: linseed, oil sunflower seed kernel and hemp seed.
The addition amount of the linseed oil accounts for 2.0-5.5%, preferably 2.0-4.0% of the total weight of the dry matters of the ration, the addition amount of the grape seed oil accounts for 0.5-5.5%, preferably 1.5-3.5% of the total weight of the dry matters of the ration, the addition amount of the linseed oil accounts for 2-10%, preferably 3.3-10% of the total weight of the dry matters of the ration, the addition amount of the oil sunflower seed kernel accounts for 0.25-2.5%, preferably 1-2.5% of the total weight of the dry matters of the ration, and the addition amount of the hemp seed accounts for 1-5%, preferably 2.5-5% of the total weight of the dry matters of the ration.
If the addition amount of the vegetable oil or the vegetable oil seeds is too small, the effect of improving the CLA content in the milk cannot be realized; if the addition amount is too high, the adverse effect on the body health and the production performance of the dairy cow is possibly generated, but the generation of CLA is inhibited, and the CLA content in the milk is reduced.
In the present invention, the vegetable oil is preferably microencapsulated powder formed by coating processing so as to control the release rate and time. The microcapsule powder has a fat content of 55% or more by weight, and linoleic acid accounts for 60% or more of the total fatty acids. The method for preparing the microcapsule powder is not particularly limited, and any coating method commonly used in the art may be used.
Preferably, the feed of the present invention is constituted by adding two or three selected from the group consisting of the vegetable oil and the vegetable oil seed to the basal diet of the dairy cow. From the viewpoint of increasing the CLA content and reducing adverse effects on the body health and productivity of cows, it is preferable to add grape seed oil to the basal ration of cows and further add at least one of the remaining vegetable oils or vegetable oil seeds, for example: combinations of grapeseed oil with linseed oil, combinations of grapeseed oil with linseed and oil sunflower seed kernels, and the like.
Preferably, in the feed of the present invention, two or three of the above-mentioned vegetable oil seeds are added to the basal ration of dairy cows. For example, the dairy cow basic ration may be added with the flaxseed and the oil sunflower seed kernel, the dairy cow basic ration may be added with the flaxseed and the hemp seed, the dairy cow basic ration may be added with the oil sunflower seed kernel and the hemp seed, and the dairy cow basic ration may be added with the flaxseed, the oil sunflower seed kernel and the hemp seed.
The feed can be further added with a nutritional function bag, wherein the nutritional function bag comprises 15-50 parts by weight of lipase, 0-20 parts by weight of vitamin E and 0-75 parts by weight of organic selenium, and the addition amount of the nutritional function bag accounts for 0.01-0.3% of the total weight of dry matters of the daily ration.
The feed disclosed by the invention is added with the vegetable oil and/or the vegetable oil seeds and the nutritional functional bag, the vegetable oil powder or the vegetable oil seeds and the lipase in the nutritional functional bag are optimally combined, and meanwhile, the vitamin E and/or the organic selenium are added, so that the CLA content in the milk can be further increased.
In the present invention, the dairy cow basal diet may be a basal diet formulation that is conventional in the art, and is not particularly limited, and may include, for example, corn silage, alfalfa, oat grass, flaked corn, corn flour, sugar beet granules, soybean meal, soybean hull, whole cottonseed, and concentrate, and the like.
< method of feeding >
Another embodiment of the present invention provides a feeding method for dairy cows, which is to feed the feed of the present invention to the dairy cows in the lactation period.
By adopting the feeding method, the CLA content in the milk can be improved, and compared with the method of directly adding CLA into the milk and milk products, the feeding method has better absorbability and functionality, and is simpler.
< feed additive >
Another embodiment of the present invention provides a feed additive comprising one or more selected from the group consisting of vegetable oils and vegetable oil seeds,
vegetable oil: linseed oil, grape seed oil;
vegetable oil seeds: flaxseed, oil sunflower seed kernel, hemp seed,
the addition amount of the linseed oil accounts for 2.0-5.5%, preferably 2.0-4.0% of the total weight of the dry matters of the ration, the addition amount of the grape seed oil accounts for 0.5-5.5%, preferably 1.5-3.5% of the total weight of the dry matters of the ration, the addition amount of the linseed oil accounts for 2-10%, preferably 3.3-10% of the total weight of the dry matters of the ration, the addition amount of the oil sunflower seed kernel accounts for 0.25-2.5%, preferably 1-2.5% of the total weight of the dry matters of the ration, and the addition amount of the hemp seed accounts for 1-5%, preferably 2.5-5% of the total weight of the dry matters of the ration.
By adding the feed additive into basic daily ration of the dairy cow, exogenous nutrition is added and regulated, the CLA content of the milk can be obviously improved through metabolic transformation of the dairy cow organism, and compared with common milk, the CLA content can even reach more than 50mg/100ml of milk.
The vegetable oil is preferably microcapsule powder obtained by coating, wherein the microcapsule powder has a fat content of 55 wt% or more and linoleic acid content of 60 wt% or more based on total fatty acids.
Preferably, the feed additive of the present invention contains two or three selected from the above-mentioned vegetable oils and the above-mentioned vegetable oil seeds. From the viewpoint of increasing the CLA content and reducing adverse effects on the physical health and productivity of cows, it is preferable to contain grapeseed oil, and further contain at least one of the remaining vegetable oils or vegetable oil seeds, for example: combinations of grapeseed oil with linseed oil, combinations of grapeseed oil with linseed and oil sunflower seed kernels, and the like.
Preferably, the feed additive of the present invention contains two or three of the above-mentioned vegetable oil seeds. For example, it may be linseed and oil sunflower seed kernel, it may be linseed and hemp seed, it may be oil sunflower seed kernel and hemp seed, it may be linseed, oil sunflower seed kernel and hemp seed.
The feed additive can further comprise a nutritional function package, wherein the nutritional function package comprises 15-50 parts by weight of lipase, 0-20 parts by weight of vitamin E and 0-75 parts by weight of organic selenium, and the addition amount of the nutritional function package in daily ration accounts for 0.01-0.3% of the total weight of dry matters of the daily ration.
By adding the nutritional functional bag, the plant oil powder or the plant oil seeds and the lipase in the nutritional functional bag are optimally combined, and simultaneously, the vitamin E and/or the organic selenium are added, so that the CLA content in the milk can be further improved.
The present invention will be described in detail below with reference to test examples, but the present invention is not limited to the following examples. The raw materials and equipment used in the following test examples were those conventionally available in the art, except for the specific ones, and "%" represents weight percent.
< milk cow basal diet >
The formula of the basal ration for dairy cows used in the following test examples is shown in Table 1, wherein alfalfa was imported from the United states, and the concentrate was obtained from 50% concentrated feed for dairy cows (trade name: Darett 7850) from Yuanhing feed company.
Table 1: dairy cow basic ration formula
< method of feeding >
The following feeding methods were used in the following test examples.
1. Test animal
Healthy lactating cows with lactation period of 90-120 days are fed to 5 cows in each group, the control group is fed with basal ration of cows, and the test group is fed with the composition of test material and basal ration of cows according to the group. The vegetable oil used in the test material was a microcapsule powder formed by coating treatment, and the microcapsule powder had a fat content of 55% or more and linoleic acid content of 60% or more of the total fatty acids. In addition, the vegetable oil seeds used in the test material were baked and parched.
2. Feeding management mode
Test herds were fed under the same nutrient feeding conditions, housed bedridden, fed three times a day (5:30, 13:30, 19:30) per TMR (total mixed ration) (5:30, 13:30, 19:30), allowed to drink water, milked side-by-side (5:00, 13:00, 19:00) 3 times a day, and morning, noon, evening milk samples were mixed at a weight ratio of 1:1: 1.
< method for collecting and detecting sample >
Milk samples were collected on days 0, 30 of the test. After the mixed milk samples of the morning, the evening and the evening of each cow are collected, the samples are respectively mixed into 1 part according to the components and stored at the temperature of minus 20 ℃ for detecting the contents of milk protein (detected according to NY/T2659-2014), milk fat (detected according to NY/T2659-2014) and CLA (detected according to NY/T1671-2008).
Blood samples were collected on days 0, 30 of the experiment. The acquisition method comprises the following steps: before morning feeding, 5mL of blood sample was collected from the tail vein into a vacuum tube coated with heparin sodium, and then centrifuged at 3000g for 10min using a centrifuge to obtain plasma, which was stored at-20 ℃ for detection of triglyceride (oxidase method), cholesterol (end point colorimetry), non-esterified fatty acids (microplate method), fatty acid synthase (ELISA method), total superoxide dismutase (hydroxylamine method), glutathione peroxidase (colorimetry), malondialdehyde (TBA method), and total antioxidant power (microplate method).
Test example 1: influence of added vegetable oil on CLA content in milk
< addition of 0.5% vegetable oil >
Test materials consisting of linseed oil and grape seed oil are prepared to serve as feed additives, the mass ratio of the linseed oil to the grape seed oil is 0:1 (test example 1-1), 2:1 (test example 1-2) and 1:0 (test example 1-3), the test materials are added into basic ration of the dairy cow, and the addition amount of the test materials accounts for 0.5% of the total weight of dry matter of the ration.
Feeding for 30 days according to the feeding method, counting the daily milk yield of each cow on the 0 th and 30 th days of the test, and taking the average value to be recorded in the table 1. The milk protein, milk fat and CLA contents in the milk of each test group, and the Triglyceride (TG), cholesterol (TC), non-esterified fatty acid (NEFA), Fatty Acid Synthase (FAS), total superoxide dismutase (T-SOD), glutathione peroxidase (GSH-PX), Malondialdehyde (MDA), and total antioxidant activity (T-AOC) indexes in serum were collected and measured according to the above sample collection and detection method. The results are shown in tables 2 and 3.
TABLE 2
As can be seen from Table 2, with respect to the CLA content in milk, the CLA content of the control group was substantially maintained even after feeding for 30 days, and the CLA content of test example 1-1 was increased by 9.81/100ml, whereas the CLA content of test example 1-2 was decreased by 0.12mg/100ml, and that of test example 1-3 was decreased by 0.04mg/100 ml.
TABLE 3
As can be seen from Table 3, the antioxidant index of blood and the related index of fat metabolism of the cow body have no significant abnormality by feeding for 30 days, which indicates that the addition of the vegetable oil has no invasive effect on the cow body after 30 days of rumen metabolism and body regulation.
< addition of 3% vegetable oil >
Preparing test materials consisting of linseed oil and grape seed oil as feed additives, wherein the mass ratio of the linseed oil to the grape seed oil is 0:1 (test examples 1-4), 2:1 (test examples 1-5) and 1:0 (test examples 1-6), and adding the test materials into basic ration of dairy cows, wherein the addition amount of the test materials accounts for 3% of the total weight of dry matters of the ration.
The CLA content in the milk of each test group was determined according to the sample collection and detection method after feeding for 30 days according to the feeding method, and the results are shown in table 4.
TABLE 4
As can be seen from Table 4, for the CLA content in milk, the CLA content in the control group was almost equal after feeding for 30 days, the CLA content in test examples 1-4 was increased by 36.27mg/100ml, the CLA content in test examples 1-5 was increased by 43.07mg/100ml, and the CLA content in test examples 1-6 was increased by 38.24mg/100 ml. In addition, in the test examples 1-4 to 1-6, the CLA content in the milk is higher than 50mg/100ml of milk after the milk is fed for 30 days.
< addition of 5.5% vegetable oil >
Test materials consisting of linseed oil and grape seed oil are prepared to serve as feed additives, the mass ratio of the linseed oil to the grape seed oil is 0:1 (test examples 1-7), the mass ratio of the linseed oil to the grape seed oil is 2:1 (test examples 1-8), and the mass ratio of the grape seed oil to serve as feed additives is 1:0 (test examples 1-9), and then the test materials are added into basic ration of dairy cows, wherein the addition amount of the test materials accounts for 5.5% of the total weight of dry matters of the daily ration.
The CLA content in the milk of each test group was determined according to the sample collection and detection method after feeding for 30 days according to the feeding method, and the results are shown in table 5.
TABLE 5
As can be seen from Table 5, the CLA content in milk was maintained at a substantially constant level in the control group after 30 days of feeding, and the CLA content in test examples 1 to 7 was increased by 24.07mg/100ml, the CLA content in test examples 1 to 8 was increased by 26.42mg/100ml, and the CLA content in test examples 1 to 9 was increased by 18.41mg/100 ml.
< addition of 7% vegetable oil >
Test materials consisting of linseed oil and grape seed oil are prepared to serve as feed additives, the mass ratio of the linseed oil to the grape seed oil is 0:1 (test examples 1-10), 2:1 (test examples 1-11) and 1:0 (test examples 1-12), and then the test materials are added into basic ration of dairy cows, wherein the addition amount of the test materials accounts for 7% of the total weight of dry matters of the ration.
The CLA content in the milk of each test group was determined according to the sample collection and detection method after feeding for 30 days according to the feeding method, and the results are shown in table 6.
TABLE 6
As can be seen from Table 6, the CLA content in milk was substantially the same in the control group after 30 days of feeding, but the CLA content in each test group showed a decreasing trend.
In the above test groups, test examples 1-1, 1-4 to 1-9 are examples of the present invention, and the rest are comparative examples.
Test example 2: influence of added vegetable oil seeds on CLA content in milk
< addition of 0.25% vegetable oilseeds >
The test feed consisting of flaxseed, hemp seed and oil sunflower seed kernel is prepared as a feed additive, the mass ratio of the flaxseed, hemp seed and oil sunflower seed kernel is 1:0:0 (test example 2-1), 0:1:0 (test example 2-2), 0:0:1 (test example 2-3), 1:1:1 (test example 2-4), 1:0:1 (test example 2-5), 0:1:1 (test example 2-6) and 1:1:0 (test example 2-7), and the feed additive is added into the basic daily ration of the dairy cow, and the addition amount of the test feed accounts for 0.25% of the total weight of the dry matter of the daily ration.
The CLA content in the milk of each test group was determined according to the sample collection and detection method after feeding for 30 days according to the feeding method, and the results are shown in table 7.
TABLE 7
As can be seen from Table 7, the CLA content in milk was maintained at a substantially constant level in the control group after 30 days of feeding, and the CLA content in test examples 2 to 3 was increased by 2.3mg/100ml, while the CLA content in the other test groups was decreased.
< addition of 1% vegetable oil seeds >
A test material consisting of flaxseed, hemp seed and oil sunflower seed kernel is prepared as a feed additive, the mass ratio of the flaxseed, the hemp seed and the oil sunflower seed kernel is 1:0:0 (test example 2-8), 0:1:0 (test example 2-9), 0:0:1 (test example 2-10), 1:1:1 (test example 2-11), 1:0:1 (test example 2-12), 0:1:1 (test example 2-13) and 1:1:0 (test example 2-14), and the test material is added into a basic ration of the dairy cow, wherein the addition amount of the test material accounts for 1% of the total weight of the dry matter of the basic ration.
The CLA content in the milk of each test group was determined according to the sample collection and detection method after feeding for 30 days according to the feeding method, and the results are shown in table 8.
TABLE 8
As can be seen from Table 8, for the CLA content in milk, the CLA content in the control group was substantially equal after 30 days of feeding, the CLA content in test examples 2 to 9 was increased by 13.96mg/100ml, the CLA content in test examples 2 to 10 was increased by 12.81mg/100ml, the CLA content in test examples 2 to 12 was increased by 7.32mg/100ml, the CLA content in test examples 2 to 13 was increased by 7.25mg/100ml, and the CLA content in the other test groups showed a decreasing trend.
< addition of 5% vegetable oil seeds >
Preparing a test material consisting of flaxseed, hemp seed and oil sunflower seed kernel as a feed additive, wherein the mass ratio of the flaxseed, the hemp seed and the oil sunflower seed kernel is 1:0:0 (test example 2-15), 0:1:0 (test example 2-16), 0:0:1 (test example 2-17), 1:1:1 (test example 2-18), 1:0:1 (test example 2-19), 0:1:1 (test example 2-20) and 1:1:0 (test example 2-21), and adding the test material into a basic ration of the dairy cow, wherein the addition amount of the test material accounts for 5% of the total weight of the dry matter of the basic ration.
The CLA content in the milk of each test group was determined according to the sample collection and detection method after feeding for 30 days according to the feeding method, and the results are shown in table 9.
TABLE 9
As can be seen from Table 9, with respect to the CLA content in milk, after 30 days of feeding, the CLA content of the control group was substantially maintained, the CLA content of test examples 2 to 15 was increased by 30.82mg/100ml, the CLA content of test examples 2 to 16 was increased by 25.92mg/100ml, the CLA content of test examples 2 to 17 was increased by 34.77mg/100ml, the CLA content of test examples 2 to 18 was increased by 31.39mg/100ml, the CLA content of test examples 2 to 19 was increased by 15.07mg/100ml, the CLA content of test examples 2 to 20 was increased by 14.54mg/100ml, and the CLA content of test examples 2 to 21 was increased by 30.58mg/100 ml.
< addition of 10% vegetable oil seeds >
The test feed consisting of flaxseed, hemp seed and oil sunflower seed kernel is prepared as a feed additive, the mass ratio of the flaxseed, hemp seed and oil sunflower seed kernel is 1:0:0 (experimental example 2-22), 0:1:0 (experimental example 2-23), 0:0:1 (experimental example 2-24), 1:1:1 (experimental example 2-25), 1:0:1 (experimental example 2-26), 0:1:1 (experimental example 2-27) and 1:1:0 (experimental example 2-28), and the feed additive is added into the basic daily ration of the dairy cow, and the addition amount of the test feed accounts for 10% of the total weight of the dry matter of the daily ration.
The CLA content in the milk of each test group was measured according to the sample collection and detection method after feeding for 30 days according to the feeding method, and the results are shown in table 10.
Watch 10
As can be seen from Table 10, with respect to the CLA content in milk, the CLA content of the control group was substantially maintained after 30 days of feeding, and the CLA content of test examples 2 to 22 was increased by 46.04mg/100ml, the CLA content of test examples 2 to 25 was increased by 51.16mg/100ml, the CLA content of test examples 2 to 26 was increased by 25.09mg/100ml, the CLA content of test examples 2 to 27 was increased by 24.43mg/100ml, and the CLA content of test examples 2 to 28 was increased by 46.04mg/100 ml. However, the CLA contents of test examples 2 to 23 and test examples 2 to 24 showed a tendency to decrease.
In the above test groups, test examples 2-3, 2-9 to 2-10, 2-12, 2-15 to 2-22, and 2-25 to 2-28 are examples of the present invention, and the rest are comparative examples.
Test example 3: influence of adding vegetable oil seed, vegetable oil and nutritional functional bag on milk CLA content
The test material consisting of flaxseed, grape seed oil, oil sunflower seed kernel and a nutritional functional bag is prepared and used as a feed additive, and the feed additive is added into basic ration of dairy cows according to the addition amount of the following test groups, wherein the nutritional functional bag comprises 50 parts by weight of lipase, 5 parts by weight of vitamin E and 45 parts by weight of organic selenium. The test components were as follows.
Test example 3-1: 3.3% of flaxseed, 1% of grape seed oil, 3.3% of oil sunflower seed kernel and 0.01% of nutritional functional bag
Test example 3-2: 3.3% of flaxseed, 1% of grape seed oil, 3.3% of oil sunflower seed kernel and 0.05% of nutritional functional bag
Test examples 3 to 3: 3.3% of flaxseed, 1% of grape seed oil, 3.3% of oil sunflower seed kernel and 0.1% of nutritional functional bag
Test examples 3 to 4: 3.3% of flaxseed, 1% of grape seed oil, 3.3% of oil sunflower seed kernel and 0.3% of nutritional functional bag
Test examples 3 to 5: 3.3% of flaxseed, 1% of grape seed oil, 3.3% of oil sunflower seed kernel and 0.5% of nutritional functional bag
The milk was fed for 30 days according to the above feeding method, and the CLA content in the milk of each test group was measured according to the above sample collection and detection method, and the results are shown in table 11.
TABLE 11
As can be seen from Table 11, with respect to the CLA content in milk, the CLA content of the control group remained substantially the same after 30 days of feeding, the CLA content of test example 3-1 was increased by 40.99mg/100ml, the CLA content of test example 3-2 was increased by 37.45mg/100ml, the CLA content of test example 3-3 was increased by 36.15mg/100ml, and the CLA content of test example 3-4 was increased by 40.16mg/100ml, but the CLA content of test example 3-5 showed a decrease.
In the above test groups, test examples 3-1 to 3-4 are examples of the present invention, and the rest are comparative examples.
Test example 4: influence of adding vegetable oil and nutritional functional bag on milk CLA content
The test material consisting of linseed oil, grape seed oil and a nutritional functional packet is prepared and used as a feed additive, and the test material is added into basic ration of the dairy cow according to the addition amount of the following test groups, wherein the nutritional functional packet comprises 15 parts by weight of lipase, 10 parts by weight of vitamin E and 75 parts by weight of organic selenium. The test components were as follows.
Test example 4-1: 2% of linseed oil, 1% of grape seed oil and 0.01% of nutritional functional bag
Test example 4-2: 2% of linseed oil, 1% of grape seed oil and 0.05% of nutritional functional bag
Test examples 4 to 3: 2% of linseed oil, 1% of grape seed oil and 0.1% of nutritional functional bag
Test examples 4 to 4: 2% of linseed oil, 1% of grape seed oil and 0.3% of nutritional functional bag
Test examples 4 to 5: 2% of linseed oil, 1% of grape seed oil and 0.5% of nutritional functional bag
The CLA content in the milk of each test group was determined according to the sample collection and detection method after feeding for 30 days according to the feeding method, and the results are shown in table 12.
TABLE 12
As can be seen from Table 12, with respect to the CLA content in milk, the CLA content in the control group was substantially maintained after 30 days of feeding, and the CLA content in test example 4-1 was increased by 45.97mg/100ml, the CLA content in test example 4-2 was increased by 47.41mg/100ml, the CLA content in test example 4-3 was increased by 45.89mg/100ml, and the CLA content in test example 4-4 was increased by 49.87mg/100ml, but the CLA content in test example 4-5 exhibited a decreasing tendency.
In the above test groups, test examples 4-1 to 4-4 are examples of the present invention, and the rest are comparative examples.
Test example 5: influence of adding vegetable oil seed and nutritional functional bag on milk CLA content
The test material consisting of flaxseed, hemp seed, oil sunflower seed kernel and a nutritional functional bag is prepared and used as a feed additive, and the test material is added into basic ration of dairy cows according to the addition amount of the following test groups, wherein the nutritional functional bag comprises 30 parts by weight of lipase, 20 parts by weight of vitamin E and 50 parts by weight of organic selenium. The test components were as follows.
Test example 5-1: 3.3% of flaxseed, 3.3% of hemp seed, 3.3% of oil sunflower seed kernel and 0.01% of nutritional functional bag
Test example 5-2: 3.3% of flaxseed, 3.3% of hemp seed, 3.3% of oil sunflower seed kernel and 0.05% of nutritional functional bag
Test examples 5 to 3: 3.3% of flaxseed, 3.3% of hemp seed, 3.3% of oil sunflower seed kernel and 0.1% of nutritional functional bag
Test examples 5 to 4: 3.3% of flaxseed, 3.3% of hemp seed, 3.3% of oil sunflower seed kernel and 0.3% of nutritional functional bag
Test examples 5 to 5: 3.3% of flaxseed, 3.3% of hemp seed, 3.3% of oil sunflower seed kernel and 0.5% of nutritional functional bag
The CLA content in the milk of each test group was measured according to the sample collection and detection method after feeding for 30 days according to the feeding method, and the results are shown in table 13. The feeding was continued, and on the 90 th day of the test, CLA content in the milk of each test group was collected and measured according to the above-mentioned sample collection and detection method, and the results are shown in Table 14. After 90 days of feeding, feeding was stopped, and a milk sample 30 days after stopping feeding was collected, and the CLA content in the milk of each test group was measured according to the above sample collection and detection methods, and the results are shown in table 15.
Watch 13
As can be seen from Table 13, with respect to the CLA content in milk, the CLA content of the control group remained substantially the same after 30 days of feeding, the CLA content of test example 5-1 was increased by 56.22mg/100ml, the CLA content of test example 5-2 was increased by 52.66mg/100ml, the CLA content of test example 5-3 was increased by 51.12mg/100ml, and the CLA content of test example 5-4 was increased by 55.06mg/100ml, but the CLA content of test example 5-5 showed a decrease.
TABLE 14
As can be seen from Table 14, with respect to the CLA content in milk, the CLA content of the control group was substantially maintained after 90 days of feeding, and the CLA content of test example 5-1 was increased by 76mg/100ml, the CLA content of test example 5-2 was increased by 72.45mg/100ml, the CLA content of test example 5-3 was increased by 71.33mg/100ml, and the CLA content of test example 5-4 was increased by 75.09mg/100ml, but the CLA content of test example 5-5 exhibited a decreasing tendency.
Watch 15
As can be seen from Table 15, after 30 days of stopping feeding, the CLA content of the control group was substantially maintained, and the CLA content of test example 5-1 was increased by 4.44mg/100ml, that of test example 5-2 by 3.67mg/100ml, that of test example 5-3 by 3.91mg/100ml and that of test example 5-4 by 3.28mg/100ml, but the CLA content of test example 5-5 exhibited a decreasing tendency.
In the above test groups, test examples 5-1 to 5-4 are examples of the present invention, and the rest are comparative examples.
The present invention has been described in further detail with reference to the foregoing embodiments, and it should be understood that the above embodiments are only illustrative of the present invention and are not intended to limit the present invention, and any modification, equivalent replacement, or improvement made within the spirit and principle of the present invention should be included in the scope of the present invention.
Claims (10)
1. A feed for increasing conjugated linoleic acid content in milk is prepared by adding one or more of the following vegetable oils and vegetable oil seeds into basal diet of milk cow,
vegetable oil: linseed oil, grape seed oil;
vegetable oil seeds: flaxseed, oil sunflower seed kernel, hemp seed,
the addition amount of the linseed oil accounts for 2.0-5.5% of the total weight of the dry matters of the daily ration, the addition amount of the grape seed oil accounts for 0.5-5.5% of the total weight of the dry matters of the daily ration, the addition amount of the linseed oil accounts for 2-10% of the total weight of the dry matters of the daily ration, the addition amount of the oil sunflower seed kernel accounts for 0.25-2.5% of the total weight of the dry matters of the daily ration, and the addition amount of the hemp seed accounts for 1-5% of the total weight of the dry matters of the daily ration.
2. The feed according to claim 1, wherein two or three selected from the group consisting of the vegetable oil and the vegetable oil seed are added to the dairy cow basal diet, preferably the flaxseed oil and the grapeseed oil are added to the dairy cow basal diet, and more preferably the grapeseed oil, the flaxseed oil and the oil sunflower seed kernel are added to the dairy cow basal diet.
3. The feed according to claim 1, which is constituted by adding two or three of the vegetable oil seeds to the basal ration of the dairy cow.
4. The feed according to any one of claims 1 to 3, further comprising a nutritional functional bag, wherein the nutritional functional bag comprises 15 to 50 parts by weight of lipase, 0 to 20 parts by weight of vitamin E, and 0 to 75 parts by weight of organic selenium,
the addition amount of the nutritional functional bag accounts for 0.01-0.3% of the total weight of the dry matters of the daily ration.
5. The feed according to claim 1 or 2, wherein the vegetable oil is a microencapsulated powder formed by coating processing, the microencapsulated powder has a fat content of 55% or more and linoleic acid content of 60% or more of the total fatty acids, on a weight basis.
6. The feed of claim 1, the dairy cow basal ration comprising corn silage, alfalfa, oat grass, flaked corn, corn meal, sugar beet pellets, soybean meal, soybean hulls, whole cotton seeds, and concentrates.
7. A feeding method for increasing the content of conjugated linoleic acid in milk, which is used for feeding the feed of any one of claims 1-6 to dairy cows in a lactation period.
8. A feed additive comprises one or more kinds selected from the group consisting of vegetable oil and vegetable oil seed,
vegetable oil: linseed oil, grape seed oil;
vegetable oil seeds: flaxseed, oil sunflower seed kernel, hemp seed,
the addition amount of the linseed oil in the daily ration accounts for 2.0-5.5% of the total weight of the dry matter of the daily ration, the addition amount of the grape seed oil in the daily ration accounts for 0.5-5.5% of the total weight of the dry matter of the daily ration, the addition amount of the linseed in the daily ration accounts for 2-10% of the total weight of the dry matter of the daily ration, the addition amount of the oil sunflower seed kernel in the daily ration accounts for 0.25-2.5% of the total weight of the dry matter of the daily ration, and the addition amount of the hemp seed in the daily ration accounts for 1-5% of the total weight of the dry matter of the daily ration.
9. The feed additive according to claim 8, further comprising a nutritional functional bag, wherein the nutritional functional bag comprises 15 to 50 parts by weight of lipase, 0 to 20 parts by weight of vitamin E, and 0 to 75 parts by weight of organic selenium,
the addition amount of the nutritional functional bag in the daily ration accounts for 0.01-0.3% of the total weight of the dry matters of the daily ration.
10. The feed additive according to claim 8, wherein the vegetable oil is a microcapsule powder formed by coating processing, the microcapsule powder has a fat content of 55% or more by weight, and linoleic acid is 60% or more of the total fatty acids.
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