CN114514922A - Isolated heart normal atmospheric temperature mechanical perfusion device - Google Patents

Abstract

The invention relates to a normal-temperature mechanical perfusion device for an isolated heart. The invention comprises an organ storage box, a blood storage tank, a membrane oxygenator and a heating water tank, wherein the blood storage tank is provided with two blood inlet and storage tank interfaces and one blood outlet and storage tank interface, the organ storage box is provided with a right chamber drainage tube, a left chamber drainage tube and an aorta root perfusion tube, the organ storage box is respectively connected with the two blood inlet and storage tank interfaces through the right chamber drainage tube and the left chamber drainage tube, the membrane oxygenator is provided with a membrane lung oxygen interface, the membrane lung blood-storing device comprises a membrane lung water outlet interface, a membrane lung water inlet interface, a membrane lung bleeding interface and a membrane lung blood inlet interface, wherein the membrane lung blood inlet interface is connected with a blood outlet storage tank interface, the membrane lung bleeding interface is connected with an organ preservation box through an aorta root perfusion tube, a heating water tank water return connector and a heating water tank water outlet connector are arranged on a heating water tank, the heating water tank water outlet connector is connected with the membrane lung water inlet interface, and the heating water tank water return connector is connected with the membrane lung water outlet interface. The invention continuously supplies oxygen and nutrition to the heart in a perfusion mode, so that the heart can maintain normal physiological functions and prolong the in vitro preservation time.

Description

A normal temperature mechanical perfusion device for isolated hearts

technical field

The invention relates to the field of medical devices, in particular to an isolated heart normal temperature mechanical perfusion device.

Background technique

Heart transplantation is an effective method for the treatment of end-stage heart disease. At present, the donor hearts are preserved in vitro by the method of organ preservation liquid cold preservation, but the effective storage period is only 6 hours, which greatly limits the development of heart transplantation. Normal-temperature mechanical perfusion of the heart refers to perfusing the isolated heart with normal-temperature oxygenated blood by mechanical perfusion to simulate the in vivo environment, thereby realizing the normal physiological function of the heart in the in vitro environment. This organ preservation method is expected to greatly improve the in vitro preservation time of the heart, shorten the cold ischemia time and reduce postoperative complications of transplantation. At present, there is no effective mechanical perfusion device for the heart at normal temperature.

SUMMARY OF THE INVENTION

In order to solve the above-mentioned technical problems existing in the background art, the purpose of the present invention is to develop an isolated heart normal temperature mechanical perfusion device, so as to realize the normal beating of the heart and maintain other physiological functions in an isolated environment. The heart is continuously supplied with oxygen and nutrients by perfusion, so that it can maintain normal physiological function and prolong its in vitro storage time; various biochemical indicators of the heart can be monitored in real time to evaluate whether it is suitable for heart transplantation.

The technical solution of the present invention is as follows: the present invention is an isolated heart normal temperature mechanical perfusion device, which is special in that the perfusion device includes an organ preservation box, a blood storage tank, a membrane oxygenator and a heating water tank, and the storage The blood tank is provided with two inlet blood storage tank interfaces and one outlet blood storage tank interface. The organ preservation box is provided with a right ventricular drainage tube, a left ventricular drainage tube and a perfusion tube at the root of the aorta, and the organ preservation box passes through the right ventricular drainage tube. The membrane oxygenator is provided with a membrane lung oxygen interface, a membrane lung water outlet, a membrane lung water inlet, a membrane lung hemorrhage interface, and a membrane lung blood inlet interface. The membrane lung blood inlet interface is connected with the blood storage tank interface, the membrane lung hemorrhage interface is connected with the organ preservation box through the aortic root perfusion tube, the heating water tank is provided with a heating water tank return water connector and a heating water tank outlet connector, and the heating water tank outlet connector is connected to the organ preservation box. The membrane lung water inlet interface is connected, and the heating water tank return water connector is connected with the membrane lung water outlet interface.

Further, the heating water tank is also provided with a water outlet for the heating water tank and a water adding port for the heating water tank, a heating rod, a temperature-sensitive probe and a submersible pump are arranged in the heating water tank, and a water outlet of a pump head is arranged on the submersible pump, and the submersible pump passes through the pump head. The water outlet is connected with the water outlet joint of the heating water tank.

Further, the membrane lung water outlet interface and the membrane lung water inlet interface are respectively arranged on both sides of the bottom of the membrane oxygenator, the membrane lung hemorrhage interface is arranged at the bottom center of the membrane oxygenator, and the membrane lung blood inlet interface is arranged on the membrane oxygenator. On the upper side and the other side above the membrane oxygenator, there is a membrane lung oxygen interface.

Further, a peristaltic pump is arranged between the membrane lung blood inlet interface and the blood storage tank outlet interface.

Further, a flow monitoring device is provided on the aortic root perfusion tube.

Further, a pressure monitoring device is provided on the aortic root perfusion tube.

Further, the perfusion device further includes a perfusion housing, the organ preservation box is arranged above the perfusion housing, the blood storage tank and the membrane oxygenator are respectively arranged on the sides of the perfusion housing, and the heating water tank is arranged in the perfusion housing.

Further, the blood storage tank is arranged on the perfusion housing through the blood tank fixing device, and the membrane oxygenator is arranged on the perfusion housing through the membrane lung fixing device.

Further, the perfusion shell is provided with a heating water tank water outlet interface and a heating water tank return water interface, the heating water tank water outlet connector is connected to the membrane lung water inlet interface through the heating water tank water outlet interface, and the heating water tank return water connector is connected to the membrane lung through the heating water tank return water interface. Water outlet connection.

Further, the perfusion device also includes a control platform, the control platform includes a control display screen and a central processing unit, the control display screen is connected with the central processing unit, and the central processing unit is respectively connected with the temperature-sensitive probe, the submersible pump, the heating rod, the flow monitoring device and the pressure. The monitoring device is connected, the control display screen is arranged above the perfusion casing, the central processing unit is connected with a power switch, and the power switch is arranged below the perfusion casing.

The isolated heart normal temperature mechanical perfusion device provided by the present invention realizes the normal beating of the heart and maintains other physiological functions in an isolated environment. The heart is continuously supplied with oxygen and nutrients by perfusion, so that it can maintain normal physiological function and prolong its in vitro storage time; various biochemical indicators of the heart can be monitored in real time to evaluate whether it is suitable for heart transplantation. It has the following advantages:

1. The present invention adopts peristaltic pump pulsatile perfusion, which can provide a perfusion method that approximates the physiology of coronary artery.

2. The present invention is equipped with flow and pressure sensors, which can monitor the flow and perfusion pressure at the aortic end in real time, and display it in real-time feedback on the control panel; at the same time, when the flow or pressure exceeds the threshold, an alarm can be issued. The flow sensor can monitor temperature and bubble alarm at the same time.

3. The membrane oxygenator can be connected in parallel with a blood perfusion device to remove inflammatory factors in the circulatory system, thereby improving the survival rate after heart transplantation; in addition, an ultrafiltration device can also be connected in parallel, when the perfusate hematocrit is too low or sodium When the potassium ion concentration is too high, ultrafiltration can be used to increase the hemoglobin concentration or reduce the sodium and potassium ion concentration to maintain the stability of the internal environment in the circulatory system.

Description of drawings

Fig. 1 is the structural representation of the present invention;

Fig. 2 is the right side view of the present invention;

Fig. 3 is the rear side view of the present invention;

Fig. 4 is the heating water tank schematic diagram of the present invention;

FIG. 5 is a schematic diagram of the application of the present invention.

The reference numerals are explained as follows:

1. Left pulmonary artery; 2. Right ventricular drainage; 3. Left superior pulmonary vein; 4. Left atrium; 5. Left inferior pulmonary vein; 6. Left ventricular drainage; 7. Left ventricle; 8. Inferior vena cava; 10. Right lower pulmonary vein; 11. Right upper pulmonary vein; 12. Right pulmonary artery; 13. Superior vena cava; 14. Aortic root perfusion tube; 15. Aorta; 16. Organ storage box; 17. Peristaltic pump; 18. Storage Blood tank interface; 19. Blood tank fixing device; 20. Blood storage tank; 21. Outlet blood storage tank interface; 22. Membrane lung oxygen interface; 23. Heating water tank outlet interface; 24. Heating water tank return water interface; 25. Membrane Lung water outlet; 26, Membrane lung water inlet; 27, Membrane oxygenator; 28, Membrane lung fixation device; 29, Membrane lung hemorrhage; 30, Membrane lung blood inlet; 31, Flow monitoring device; 32, Pressure Monitoring device; 33. Control display screen; 34. Power switch; 35. Heated water tank return water connector; 36. Heated water tank outlet connector; 37. Heated water tank; 38. Temperature sensitive probe; 39. Pump head water outlet; Pump; 41. Water outlet for heating water tank; 42. Heating rod; 43. Water inlet for heating water tank.

Detailed ways

Below in conjunction with the accompanying drawings and specific embodiments, the overall scheme of the present invention is described in further detail:

1, 2, 3, and 4, the structure of the specific embodiment of the present invention includes an organ preservation box 16, a blood storage tank 20, a membrane oxygenator 27 and a heating water tank 37. The membrane oxygenator 27 is the membrane of the membrane lung Oxygenator. The blood storage tank 20 is provided with two inlet blood storage tank interfaces 18 and one outlet blood storage tank interface 21, and the organ preservation box 16 is provided with a right ventricular drainage tube 2, a left ventricular drainage tube 6 and aortic root perfusion tube 14, The organ preservation box 16 is respectively connected with the two inlet blood storage tank ports 18 through the right ventricular drainage tube 2 and the left ventricular drainage tube 6, and the membrane oxygenator 27 is provided with a membrane lung oxygen interface 22, a membrane lung water outlet interface 25, a membrane oxygen outlet The lung water inlet port 26, the membrane lung hemorrhage port 29 and the membrane lung blood inlet port 30, the membrane lung water outlet port 25 and the membrane lung water inlet port 26 are respectively arranged on both sides of the bottom of the membrane oxygenator 27, and the membrane lung hemorrhage port 29 is arranged on the membrane In the center of the bottom of the membrane oxygenator 27, the membrane lung blood inlet interface 30 is arranged on one side above the membrane oxygenator 27, and the membrane lung oxygen interface 22 is arranged on the other side above the membrane oxygenator 27. The heating water tank 37 is provided with a heating water tank return water joint 35 and a heating water tank water outlet joint 36, wherein the membrane lung blood inlet interface 30 is connected with the blood storage tank interface 21, and the membrane lung hemorrhage interface 29 is connected to the organ through the aortic root perfusion tube 14. The storage tank 16 is connected, the water outlet connector 36 of the heating water tank is connected to the water inlet port 26 of the membrane lung, and the return water connector 35 of the heating water tank is connected 25 to the water outlet port of the membrane lung. A peristaltic pump 17 is provided between the membrane lung blood inlet port 30 and the blood storage tank outlet port 21 . The aortic root perfusion tube 14 is provided with a flow monitoring device 31 and a pressure monitoring device 32 .

The heating water tank 37 is also provided with a heating water tank water discharge port 41 and a heating water tank water filling port 43 respectively. The heating water tank 37 is provided with a heating rod 42, a temperature sensitive probe 38 and a submersible pump 40, and the submersible pump 40 is provided with a pump head water outlet 39. , the submersible pump 40 is connected to the water outlet joint 36 of the heating water tank through the water outlet 39 of the pump head.

In this embodiment, the present invention also includes a perfusion housing, the organ preservation box 16 is arranged above the perfusion housing, the blood storage tank 20 and the membrane oxygenator 27 are respectively arranged on the side of the perfusion housing, and the heating water tank 37 is arranged on the perfusion housing. inside the casing. The blood storage tank 20 is arranged on the perfusion housing through the blood tank fixing device 19 , and the membrane oxygenator 27 is arranged on the perfusion housing through the membrane lung fixing device 28 . The perfusion shell is also provided with a heating water tank outlet port 23 and a heating water tank return water port 24. The heating water tank outlet port 36 is connected to the membrane lung water inlet port 26 through the heating water tank water outlet port 23, and the heating water tank return water connector 35 returns water through the heating water tank. The interface 24 is connected to the membrane lung water outlet interface 25 .

The present invention also includes a control platform, the control platform includes a control display screen 33 and a central processing unit, the control display screen 33 is connected with the central processing unit, and the central processing unit is respectively connected with the temperature-sensitive probe 38, the submersible pump 40, the heating rod 42, and the flow monitoring device. 31 is connected to the pressure monitoring device 32, the control display screen 33 is arranged above the perfusion housing, the central processing unit is connected to a power switch 34, and the power switch 34 is arranged below the perfusion housing.

Referring to Figure 5, the perfusion preservation solution used in the present invention is mainly prepared from diluted red blood cells and colloid, which can provide oxygen and energy to the isolated heart; the red blood cells used are of the same blood type as the perfused heart. The remaining components of the perfusate include: albumin, calcium chloride, sodium bicarbonate, magnesium sulfate, potassium chloride, sodium glycerophosphate, sodium chloride, glucose, sodium heparin, antibiotics, compound amino acids, multivitamins, and insulin. In the preparation process, the above-mentioned components and the concentrated red blood cells of the same blood type of the isolated heart are diluted to an appropriate ratio, and the physiological osmotic pressure is maintained.

The specific usage mode of the present invention is as follows:

1) Heart acquisition and intubation;

The bag was sutured at the root of the aorta, the large blood vessels were cut off after cold perfusion cardiac arrest, and the inferior vena cava 8, superior vena cava 13, right inferior pulmonary vein 10, right superior pulmonary vein 11, left superior pulmonary vein 3 and right pulmonary artery 12 were ligated respectively. Pulmonary artery 1 or main pulmonary artery is inserted into right ventricular drainage tube 2 to right ventricle 9; left ventricular drainage tube 6 is inserted from left inferior pulmonary vein 5 to left ventricle 7, namely left atrium 4; aortic root perfusion tube 14 is inserted from aorta 15 and ligated fixed.

2) Organ perfusion

Using the perfusion tube connection, the perfusate is sequentially connected to the blood storage tank interface 18 through the right ventricular drainage tube 2 and the left ventricular drainage tube 6 in the organ preservation box 16 into the blood storage tank 20, and through the outlet of the blood storage tank interface 21, the peristaltic pump. 17 and the membrane lung blood inlet port 30 enter the membrane oxygenator 27 for temperature change and oxygenation, wherein the membrane lung water inlet port 26 is connected to the heated water tank outlet connector 36 through the heating water tank outlet port 23, and the membrane lung water outlet port 25 is heated by The water tank return interface 24 and the heating water tank return water joint 35 ensure that the membrane lung is connected to the heating water tank, and the hot water on both sides is responsible for heating the perfusion fluid in the center of the membrane oxygenator 27 to achieve its temperature-changing function; at the same time, the membrane lung The oxygen interface 22 is connected with an external oxygen source to ensure the oxygen supply of the membrane oxygenator 27 . The perfusate after oxygenation and temperature change flows out of the membrane lung from the membrane lung hemorrhage interface 29 through the flow monitoring device 31 and the pressure monitoring device connection 32 and the aortic root perfusion tube 14 in the organ preservation box 16. Infuse 15. The pressure and flow data monitored by the system can be displayed on the control display screen 33 in real time, and the rotational speed of the peristaltic pump 17 can be adjusted to adjust the system flow. When the arterial pressure or flow exceeds the threshold, the system will alarm.

The membrane oxygenator 27 can be connected in parallel with a blood perfusion device to remove inflammatory factors in the circulatory system, thereby improving the survival rate after heart transplantation; in addition, an ultrafiltration device can also be connected in parallel, when the perfusion fluid hematocrit is too low or sodium potassium When the ion concentration is too high, ultrafiltration can be used to increase the concentration of hemoglobin or reduce the concentration of sodium and potassium ions to maintain the stability of the internal environment in the circulatory system.

The heating water tank 37 can be filled with water through the heating water tank water filling port 43 , or can be discharged from the heating water tank water discharge port 41 . After the equipment is turned on, the temperature of the water tank is set on the control display screen 33, the water in the heating water tank 37 is heated by the heating rod 42, and the temperature of the liquid in the heating water tank 37 is monitored by the temperature sensitive probe 38. The control display screen 33 starts the submersible pump 40, and the heated water can be connected to the heated water tank outlet 23 through the pump head water outlet 39 and the heated water tank outlet joint 36, and the perfusion fluid is subjected to temperature change treatment through the membrane oxygenator 27, and the heated water passes through The membrane lung water outlet port 25 , the heating water tank return water port 24 and the heating water tank return water joint 35 return to the heating water tank 37 to complete the self-circulation of the water in the heating water tank 37 .

The content of the present invention and the technical content not specifically described in the above embodiments are the same as the prior art.

The above are only specific embodiments disclosed in the present invention, but the protection scope disclosed in the present invention is not limited thereto, and the protection scope disclosed in the present invention should be subject to the protection scope of the claims.

Claims (10)

1. An isolated heart normal temperature mechanical perfusion device, characterized in that: the perfusion device comprises an organ preservation box, a blood storage tank, a membrane oxygenator and a heating water tank, and the blood storage tank is provided with two storage tanks. A blood tank interface and an outlet blood storage tank interface, the organ preservation box is provided with a right ventricular drainage tube, a left ventricular drainage tube and an aortic root perfusion tube, and the organ preservation box is provided with a right ventricular drainage tube and a left ventricular drainage tube They are respectively connected with two inlet blood storage tank interfaces, and the membrane oxygenator is provided with a membrane lung oxygen interface, a membrane lung water outlet interface, a membrane lung water inlet interface, a membrane lung hemorrhage interface and a membrane lung blood inlet interface. The pulmonary blood inlet interface is connected with the outlet blood storage tank interface, the membrane pulmonary hemorrhage interface is connected with the organ preservation box through the aortic root perfusion tube, the heating water tank is provided with a heating water tank return water joint and a heating water tank water outlet joint, the The water outlet joint of the heating water tank is connected with the water inlet interface of the membrane lung, and the return water joint of the heating water tank is connected with the water outlet interface of the membrane lung. 2. The isolated heart normal temperature mechanical perfusion device according to claim 1, characterized in that: the heating water tank is also provided with a heating water tank water discharge port and a heating water tank water filling port, and the heating water tank is provided with a heating rod, A temperature-sensitive probe and a submersible pump, the submersible pump is provided with a pump head water outlet, and the submersible pump is connected with the heating water tank outlet joint through the pump head water outlet. 3. The isolated heart normal temperature mechanical perfusion device according to claim 2, wherein the membrane lung water outlet interface and the membrane lung water inlet interface are respectively arranged on both sides of the bottom of the membrane oxygenator, and the membrane lung hemorrhage interface It is arranged at the bottom center of the membrane oxygenator, the membrane lung blood inlet interface is arranged on one side above the membrane oxygenator, and the membrane lung oxygen interface is arranged on the other side above the membrane oxygenator. 4 . The isolated heart normal temperature mechanical perfusion device according to claim 3 , wherein a peristaltic pump is provided between the membrane lung blood inlet interface and the blood storage tank outlet interface. 5 . 5 . The isolated heart normal temperature mechanical perfusion device according to claim 4 , wherein the aortic root perfusion tube is provided with a flow monitoring device. 6 . 6 . The isolated heart normal temperature mechanical perfusion device according to claim 5 , wherein a pressure monitoring device is provided on the aortic root perfusion tube. 7 . 7 . The isolated heart normal temperature mechanical perfusion device according to claim 6 , wherein the perfusion device further comprises a perfusion housing, the organ preservation box is arranged above the perfusion housing, the blood storage tank and the membrane The type oxygenators are respectively arranged on the sides of the perfusion casing, and the heating water tank is arranged in the perfusion casing. 8 . The isolated heart normal temperature mechanical perfusion device according to claim 7 , wherein the blood storage tank is arranged on the perfusion housing through a blood tank fixing device, and the membrane oxygenator is arranged through a membrane lung fixing device. 9 . Set on the perfusion housing. 9 . The isolated heart normal temperature mechanical perfusion device according to claim 8 , wherein the perfusion shell is provided with a heating water tank water outlet interface and a heating water tank return water interface, and the heating water tank water outlet joint discharges water through the heating water tank. 10 . The interface is connected with the water inlet interface of the membrane lung, and the water return joint of the heating water tank is connected with the water outlet interface of the membrane lung through the water return interface of the heating water tank. 10 . The isolated heart normal temperature mechanical perfusion device according to claim 9 , wherein the perfusion device further comprises a control platform, the control platform includes a control display screen and a central processing unit, and the control display screen is connected with the central processing unit. 11 . The processor is connected, the central processing unit is respectively connected with the temperature-sensitive probe, submersible pump, heating rod, flow monitoring device and pressure monitoring device, the control display screen is arranged above the perfusion housing, and the central processing unit is connected to a power supply A switch, the power switch is arranged below the perfusion housing.

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