CN114509535B - Method for detecting maturity of ossein in bone gelatin production - Google Patents
Method for detecting maturity of ossein in bone gelatin production Download PDFInfo
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- CN114509535B CN114509535B CN202111620863.5A CN202111620863A CN114509535B CN 114509535 B CN114509535 B CN 114509535B CN 202111620863 A CN202111620863 A CN 202111620863A CN 114509535 B CN114509535 B CN 114509535B
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- 238000000034 method Methods 0.000 title claims abstract description 53
- 210000000988 bone and bone Anatomy 0.000 title claims abstract description 49
- 108010010803 Gelatin Proteins 0.000 title claims abstract description 37
- 239000008273 gelatin Substances 0.000 title claims abstract description 37
- 229920000159 gelatin Polymers 0.000 title claims abstract description 37
- 235000019322 gelatine Nutrition 0.000 title claims abstract description 37
- 235000011852 gelatine desserts Nutrition 0.000 title claims abstract description 37
- 238000004519 manufacturing process Methods 0.000 title claims abstract description 18
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 52
- 238000005406 washing Methods 0.000 claims abstract description 28
- 238000005554 pickling Methods 0.000 claims abstract description 27
- 238000001514 detection method Methods 0.000 claims abstract description 21
- 239000000499 gel Substances 0.000 claims abstract description 20
- 239000007788 liquid Substances 0.000 claims abstract description 16
- 229910052500 inorganic mineral Inorganic materials 0.000 claims description 13
- 239000011707 mineral Substances 0.000 claims description 13
- 239000002253 acid Substances 0.000 claims description 12
- 239000008399 tap water Substances 0.000 claims description 8
- 235000020679 tap water Nutrition 0.000 claims description 8
- 238000010438 heat treatment Methods 0.000 claims description 7
- 238000003809 water extraction Methods 0.000 claims description 7
- 238000003556 assay Methods 0.000 claims 2
- 238000000605 extraction Methods 0.000 abstract description 4
- 239000008213 purified water Substances 0.000 description 5
- 238000007598 dipping method Methods 0.000 description 3
- 238000002474 experimental method Methods 0.000 description 2
- 230000035800 maturation Effects 0.000 description 2
- 238000012986 modification Methods 0.000 description 2
- 230000004048 modification Effects 0.000 description 2
- 239000002994 raw material Substances 0.000 description 2
- OYPRJOBELJOOCE-UHFFFAOYSA-N Calcium Chemical compound [Ca] OYPRJOBELJOOCE-UHFFFAOYSA-N 0.000 description 1
- 239000011575 calcium Substances 0.000 description 1
- 229910052791 calcium Inorganic materials 0.000 description 1
- 239000003153 chemical reaction reagent Substances 0.000 description 1
- 238000007796 conventional method Methods 0.000 description 1
- 230000002950 deficient Effects 0.000 description 1
- 238000005238 degreasing Methods 0.000 description 1
- 239000003292 glue Substances 0.000 description 1
- 230000003472 neutralizing effect Effects 0.000 description 1
- 239000002245 particle Substances 0.000 description 1
- 229920002120 photoresistant polymer Polymers 0.000 description 1
Classifications
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
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- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Food Science & Technology (AREA)
- Medicinal Chemistry (AREA)
- Physics & Mathematics (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- General Physics & Mathematics (AREA)
- Immunology (AREA)
- Pathology (AREA)
- Investigating Or Analysing Biological Materials (AREA)
Abstract
The invention belongs to the technical field of bone gelatin production, and particularly discloses a method for detecting the maturity of bone gelatin in an pickling process in bone gelatin production. The detection method comprises the steps of firstly extracting a bone element sample from an pickling process, and washing the bone element sample; then carrying out water gel extraction treatment on the bone element sample after water washing, and detecting the gel content of the gel-containing liquid obtained by the water gel extraction treatment; judging the maturity of the ossein according to the gel content detection result. The detection method provided by the invention can accurately judge the maturity of the ossein in the pickling process, and provides a reliable basis for obtaining the qualified ossein with uniform maturity.
Description
Technical Field
The invention belongs to the technical field of bone gelatin production, and particularly relates to a method for detecting the maturity of bone gelatin in an pickling process in bone gelatin production.
Background
The existing treatment method for the bone raw material for gelatin mainly comprises the procedures of crushing bones, degreasing, sorting, pickling, liming, neutralizing, washing and the like. Wherein the pickling process is a process of converting bone particles into desired ossein. The maturity of ossein during pickling is directly related to the yield and quality of gelatin.
At present, the end point of the maturation of the bone grains is judged by using traditional manual experience detection, namely eye observation and hand grasping, and is judged by hand feeling. The existing detection method is greatly influenced by environment and personal experience, often causes that bone mineral is ripe differently in the pickling stage, and leads to different periods of the ash dipping working procedure of the produced bone gelatin and great fluctuation of product quality. Therefore, detection of bone mineral maturity is critical to enterprise sales and market competition.
Based on the method, a scientific method for detecting the maturity of the ossein is developed, and the method has important significance for improving the yield and quality of gelatin.
Disclosure of Invention
The invention mainly solves the technical problem of providing a method for detecting the maturity of bone mineral in the production of bone gelatin, which can accurately judge the maturity of bone mineral in the pickling process, provides a guarantee for obtaining qualified bone mineral, ensures that the qualified bone mineral is detected to enter the next process, can ensure the stability of the production period of the bone gelatin, the stability of the product quality and the stability of the yield, and provides a powerful guarantee for the stable production of enterprises.
In order to solve the technical problems, the invention provides a method for detecting the maturity of ossein in the production of bone gelatin, which is to detect the ossein in the pickling process, and comprises the following steps:
extracting a bone element sample from an pickling process, and performing water washing treatment on the bone element sample;
carrying out water extraction treatment on the bone element sample after water washing, and detecting the gel content of the gel-containing liquid obtained by the water extraction treatment;
judging the maturity of the ossein according to the gel content detection result.
As a preferred embodiment of the invention, the detection result of the content of the gelatin is the mass percent of the gelatin in the gelatin-containing liquid, and the content is marked as a; the step of judging the maturity of the ossein according to the detection result of the gel content comprises the following steps:
a is less than 2.0 percent, and the ossein sample is immature ossein and needs further pickling treatment;
a is more than or equal to 2.0% and less than or equal to 2.2%, and the ossein sample is mature ossein;
a > 2.2%, the ossein sample is mature excess ossein.
As a preferred embodiment of the present invention, the water-based photoresist stripping treatment comprises: and (3) placing the water-washed ossein sample into water, and performing heating treatment.
Preferably, the mass of the ossein sample after washing is 200g; and/or the number of the groups of groups,
the volume of water was 200ml and the water temperature was 55 ℃. Preferably, the water is purified water.
Preferably, the treatment temperature of the heating treatment is 55 ℃ and the treatment time is 4 hours.
As a preferred embodiment of the present invention, the water washing treatment comprises: and rinsing the ossein sample by using washing water until the difference value between the pH value of the rinsing liquid and the pH value of the washing water is less than or equal to 0.2.
Preferably, the washing water is tap water, and the water temperature is 20-25 ℃.
Further preferably, the water washing treatment includes: and (3) placing the ossein sample into a barrel, allowing water to enter from the bottom of the barrel, overflowing from the upper end of the barrel, rinsing until the difference between the pH value of rinsing liquid in the barrel and the pH value of tap water is less than or equal to 0.2, and finishing rinsing.
Preferably, the flow rate of water is 0.4 to 0.6L/min.
Preferably, the ossein sample after rinsing is placed in a barrel for glue extraction treatment; preferably, the standing time is 40 to 70 minutes.
As a preferred embodiment of the present invention, the ossein sample is extracted from a fresh acid tank of an pickling process.
The pickling process is a continuous pickling process commonly used in the existing pickling process, and comprises a plurality of acid tanks which are sequentially connected in series, such as a new acid tank, a primary new acid tank, a secondary new acid tank and the like, wherein the pickling process adopts a countercurrent dipping principle until the calcium water tank is at the tail, dilute acid is contacted with new aggregate, and the high-concentration new acid in the new acid tank is contacted with the bone mineral which is almost completely demineralized. Therefore, the ossein in the new acid tank is mature first, and can be detected only in general, and if the detection is qualified, the pickling ossein can flow into the next process, namely the liming process.
The invention also provides application of the detection method in detecting the maturity of ossein.
The detection method provided by the invention can accurately judge the maturity of the ossein in the pickling process, provides a reliable basis for obtaining the ossein with uniform maturity and qualified ossein, and the ossein which is detected to be qualified enters the liming process again. The method provided by the invention can be used for detecting the bone gelatin, so that the stability of the production period of the bone gelatin, the stability of the product quality and the stability of the yield can be ensured, and a powerful guarantee is provided for the stable production of enterprises.
Detailed Description
The technical scheme of the present invention will be clearly and completely described in connection with the specific embodiments. It will be appreciated by those skilled in the art that the embodiments described below are merely illustrative of the present invention and should not be construed as limiting the scope of the present invention. All other embodiments, e.g., modified and substituted embodiments, which can be made by one skilled in the art without the exercise of inventive faculty, are intended to be within the scope of the invention.
The experimental methods used in the following examples are all conventional methods unless otherwise specified; the raw materials, reagents, instruments and the like used are commercially available unless otherwise specified.
The invention provides a method for detecting the maturity of ossein in bone gelatin production, which comprises the following steps:
extracting a bone element sample from an pickling process, and performing water washing treatment on the bone element sample;
carrying out water extraction treatment on the bone element sample after water washing, and detecting the gel content of the gel-containing liquid obtained by the water extraction treatment;
judging the maturity of the ossein according to the gel content detection result.
The ossein sample is extracted from a new acid tank in the pickling process.
And the detection result of the content of the gelatin is the mass percentage content of the gelatin in the gelatin-containing liquid, and is marked as a. The step of judging the maturity of the ossein according to the detection result of the gel content comprises the following steps:
a is less than 2.0 percent, and the ossein sample is immature ossein and needs further pickling treatment;
a is more than or equal to 2.0% and less than or equal to 2.2%, and the ossein sample is mature ossein;
a > 2.2%, the ossein sample is mature excess ossein.
Wherein, the water extraction treatment includes: and (3) placing the water-washed ossein sample into purified water, and performing heating treatment.
Wherein the mass of the ossein sample after water washing is 200g; the volume of the purified water was 200ml and the water temperature was 55 ℃.
The treatment temperature of the heating treatment is 55 ℃, and the treatment time is 4 hours.
The water washing treatment of the ossein sample comprises: and rinsing the ossein sample by using washing water until the difference value between the pH value of the rinsing liquid and the pH value of the washing water is less than or equal to 0.2.
Preferably, the washing water is tap water, and the water temperature is 20-25 ℃.
More specifically, the water washing treatment includes: and (3) placing the ossein sample into a barrel, allowing water to enter from the bottom of the barrel, overflowing from the upper end of the barrel, rinsing until the difference between the pH value of rinsing liquid in the barrel and the pH value of tap water is less than or equal to 0.2, and finishing rinsing.
Wherein the flow rate of water is 0.4-0.6L/min.
Preferably, the ossein sample after rinsing is placed in a barrel for water gel extraction treatment. The standing time is 40-70 min.
Example 1
The embodiment provides a method for detecting the maturity of ossein in bone gelatin production, which comprises the following steps of:
(1) Taking 1kg of representative ossein samples from a new acid tank in pickling production at multiple points;
(2) Putting 1kg of bone mineral sample into a plastic barrel, feeding tap water at 20-25 ℃ from the bottom of the plastic barrel by a water inlet hose, overflowing from the upper end of the barrel, controlling the water flow speed to be 0.5L/min, continuously rinsing for 4 hours, enabling the pH value of rinsing liquid in the barrel to be equivalent to that of tap water, enabling the difference value to be not more than 0.2, and prolonging the rinsing time until the pH value meets the pH requirement if the difference value is not more than 0.2; then standing for 1h;
(3) 200g of a representative bone mineral sample is taken from the bone mineral sample after water washing, the bone mineral sample is put into a beaker, 200ml of purified water with the temperature of 55 ℃ is then measured and put into the beaker, and then the purified water and the beaker are put into a water bath with the temperature of 55 ℃ together for heating treatment, so that gelatin in the bone mineral sample is extracted;
detecting the gelatin content of the liquid in the beaker by using a detector every 0.5h, wherein the total detection time is 4h, and marking the gelatin content of the liquid in the beaker as a in percentage by mass when the last point is detected.
If a is less than 2.0%, the ossein sample is a defective ossein, and pickling is also required.
If a is between 2.0 and 2.2 percent, the ossein sample is mature ossein, and the process can enter a liming process.
If a is greater than 2.2%, this indicates that the maturation of the ossein sample is too old, which can affect the yield and quality of gelatin.
Bone gelatin production was performed using samples of ossein with a less than 2.0%, a between 2.0-2.2% and a greater than 2.2%, respectively, with performance indices shown in table 1 below.
TABLE 1
As shown in the above table, when a is less than 2.0%, the final produced gelatin is poor in quality and low in selling price. When the gel content a is more than 2.2%, the produced gelatin has high unit consumption, low yield and high cost. Gelatin is produced by adopting ossein with the gel content a of 2.0-2.2%, so that the bone consumption is low and the product quality is good.
From the above, the detection method provided by the invention can accurately judge the maturity of the ossein in the pickling process, provides a reliable basis for obtaining the ossein with uniform maturity and qualified ossein, and can effectively reduce bone consumption, improve the quality of products, and provide powerful guarantee for the stability of production cycle, the stability of product quality and the stability of yield after the qualified ossein is detected to enter the ash dipping process.
While the invention has been described in detail in the foregoing general description, embodiments and experiments, it will be apparent to those skilled in the art that modifications and improvements can be made thereto. Accordingly, such modifications or improvements may be made without departing from the spirit of the invention and are intended to be within the scope of the invention as claimed.
Claims (13)
1. The method for detecting the maturity of the bone mineral in the production of the bone gelatin is characterized by comprising the following steps of:
extracting a bone element sample from an pickling process, and performing water washing treatment on the bone element sample;
carrying out water extraction treatment on the bone element sample after water washing, and detecting the gel content of the gel-containing liquid obtained by the water extraction treatment;
judging the maturity of the ossein according to the gel content detection result.
2. The detection method according to claim 1, wherein the detection result of the content of gelatin is the mass percentage content of gelatin in the gelatin-containing liquid, denoted as a; the step of judging the maturity of the ossein according to the detection result of the gel content comprises the following steps:
a is less than 2.0 percent, and the ossein sample is immature ossein;
a is more than or equal to 2.0% and less than or equal to 2.2%, and the ossein sample is mature ossein;
a > 2.2%, the ossein sample is mature excess ossein.
3. The method according to claim 1, wherein the water-stripping treatment comprises: and (3) placing the water-washed ossein sample into water, and performing heating treatment.
4. The method according to claim 3, wherein the mass of the ossein sample after washing is 200g; and/or the number of the groups of groups,
the volume of water was 200ml and the water temperature was 55 ℃.
5. The method according to claim 3, wherein the heat treatment is performed at a treatment temperature of 55℃for a treatment time of 4 hours.
6. The method according to claim 1, wherein the water-washing treatment comprises: and rinsing the ossein sample by using washing water until the difference value between the pH value of the rinsing liquid and the pH value of the washing water is less than or equal to 0.2.
7. The method according to claim 6, wherein the washing water is tap water and the water temperature is 20-25 ℃.
8. The method according to claim 7, wherein the water-washing treatment comprises: and (3) placing the ossein sample into a barrel, allowing water to enter from the bottom of the barrel, overflowing from the upper end of the barrel, rinsing until the difference between the pH value of rinsing liquid in the barrel and the pH value of tap water is less than or equal to 0.2, and finishing rinsing.
9. The method according to claim 8, wherein the flow rate of water is 0.4 to 0.6L/min.
10. The method according to claim 8, wherein the ossein sample after rinsing is left in a tub for water-stripping treatment.
11. The method according to claim 10, wherein the standing time is 40 to 70 minutes.
12. The method according to any one of claims 1 to 11, wherein the ossein sample is extracted from a fresh acid tank of an pickling process.
13. Use of the assay of any one of claims 1 to 12 in the assay of ossein maturity.
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| CN202111620863.5A CN114509535B (en) | 2021-12-27 | 2021-12-27 | Method for detecting maturity of ossein in bone gelatin production |
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Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102329843A (en) * | 2011-08-05 | 2012-01-25 | 蚌埠丰原明胶有限公司 | Enzyme method for preparing gelatin |
| CN102732592A (en) * | 2012-07-13 | 2012-10-17 | 江南大学 | Method for preparing freshwater fish bone gelatin by enzyme process |
| WO2016145977A1 (en) * | 2015-03-14 | 2016-09-22 | 宁夏鑫浩源生物科技股份有限公司 | Enzymatic gelatin preparation process |
| CN112608686A (en) * | 2020-12-04 | 2021-04-06 | 安徽丰原明胶有限公司 | Liming method for preparing bone gelatin and application |
Family Cites Families (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20020098222A1 (en) * | 1997-03-13 | 2002-07-25 | John F. Wironen | Bone paste |
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- 2021-12-27 CN CN202111620863.5A patent/CN114509535B/en active Active
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102329843A (en) * | 2011-08-05 | 2012-01-25 | 蚌埠丰原明胶有限公司 | Enzyme method for preparing gelatin |
| CN102732592A (en) * | 2012-07-13 | 2012-10-17 | 江南大学 | Method for preparing freshwater fish bone gelatin by enzyme process |
| WO2016145977A1 (en) * | 2015-03-14 | 2016-09-22 | 宁夏鑫浩源生物科技股份有限公司 | Enzymatic gelatin preparation process |
| CN112608686A (en) * | 2020-12-04 | 2021-04-06 | 安徽丰原明胶有限公司 | Liming method for preparing bone gelatin and application |
Non-Patent Citations (1)
| Title |
|---|
| 用副产酸浸渍骨料的效果;高世理;《明胶科学与技术》;第18卷(第3期);第1页 * |
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