CN114506907A - A kind of active oxygen/active nitrogen enhanced oxidized electrolyzed water and its preparation method and application - Google Patents

Abstract

The invention belongs to the field of novel environmental protection and bactericide preparation, in particular to an active oxygen/active nitrogen enhanced oxidized electrolyzed water and a preparation method and application thereof. The method comprises the following steps: in the ion-exchange membrane electrolytic cell, the electrolytic cell is divided into two parts, an anode area and a cathode area by a homogeneous ion exchange membrane; an equal volume of sodium chloride aqueous solution is added as an electrolyte in the anode area and the cathode area, and constant current electrolysis is performed. , and the oxidized electrolyzed water is obtained in the anode area; the prepared oxidized electrolyzed water is added to the activation chamber, and nitrogen and oxygen are used as carrier gases to generate low-temperature plasma by glow discharge and spray into the oxidized electrolyzed water. Relevant chemical reactions occur, thereby preparing active oxygen/active nitrogen-enhanced oxidized electrolyzed water high-efficiency bactericide rich in active chlorine, active oxygen and active nitrogen. Active oxygen/active nitrogen enhanced oxidized electrolyzed water has high sterilization efficiency, broad sterilization spectrum, wide application range, convenient use, and a new type of bactericide that is environmentally friendly.

Description

A kind of active oxygen/active nitrogen enhanced oxidized electrolyzed water and its preparation method and application

technical field

The invention belongs to the field of novel environmental protection and bactericide preparation, and more particularly relates to an active oxygen/active nitrogen enhanced oxidized electrolyzed water and a preparation method and application thereof.

Background technique

Foodborne diseases are widespread in all parts of the world, and their morbidity ranks at the forefront of the total incidence of various diseases. It is the most prominent health problem in the world, and is directly related to human life safety and health. According to statistics, more than 80% of bacterial infections are related to bacterial biofilms in food processing equipment. Bacterial biofilms are multicellular complex microbial communities with three-dimensional self-assembled extracellular polymeric structures (exopolysaccharides, proteins, and extracellular DNA, etc.). Biofilm cells are more resistant to fungicides than planktonic cells, so they are extremely difficult to eliminate. Therefore, it is of great strategic significance to research new food sterilization technologies with wide adaptability, high efficiency, broad spectrum, safety, no residue and strong applicability.

Oxidized electrolyzed water bactericide is an electrochemical sterilization technology that has been widely studied in recent years. The sterilization efficiency of oxidized electrolyzed water is better than that of common food fungicides (sodium hypochlorite, ozone, chlorine dioxide, etc.), which is harmless to human body and has less environmental residues, so it is a safe disinfectant. However, at present, the killing effect of oxidized electrolyzed water on bacterial biofilms in food processing equipment is much lower than that on bacterial planktonic cells. This is because bacterial biofilm is a complex microbial community, and the extracellular polymers secreted by microorganisms can protect the internal cells, making it difficult for the active chlorine sterilization factor in the oxidized electrolyzed water to penetrate into the interior of the cells and complete the sterilization.

In conclusion, food-borne pathogens that persist in food processing equipment mainly grow in the form of biofilms. Biofilm cells are highly resistant to fungicides, and new types of fungicides that are both efficient and environmentally friendly are still lacking.

SUMMARY OF THE INVENTION

The purpose of the present invention is to solve the above problems, by increasing the active oxygen and active nitrogen content of the oxidized electrolyzed water, thereby obtaining enhanced oxidized electrolyzed water with both a certain active chlorine content and a certain active oxygen/active nitrogen content. The active oxygen/active nitrogen-enhanced oxidized electrolyzed water has an efficient bactericidal effect on pathogenic microorganism membranes.

In order to achieve the above object, a first aspect of the present invention provides a preparation method of active oxygen/active nitrogen enhanced oxidized electrolyzed water, the preparation method comprising:

S1: Preparation of oxidized electrolyzed water: In the ion membrane electrolytic cell, the electrolytic cell is divided into two parts, the cathode area and the anode area by using a homogeneous ion exchange membrane; sodium chloride aqueous solution is added as the electrolyte in the cathode area and the anode area respectively, and the constant current In electrolysis, acidic oxidized electrolyzed water is obtained in the anode area, and alkaline reduction electrolyzed water is obtained in the cathode area;

S2: Preparation of active oxygen/active nitrogen-enhanced oxidized electrolyzed water: place the oxidized electrolyzed water prepared in step S1 in an activation chamber, and place the plasma generator spray head above or below the oxidized electrolyzed aqueous solution; The carrier gas of nitrogen and oxygen is the excitation gas source, and a low-temperature plasma is generated by glow discharge; it is sprayed into the oxidized electrolyzed water, and chemically reacts with the oxidized electrolyzed water to obtain a plasma rich in active chlorine, active oxygen and active nitrogen. The active oxygen/active nitrogen enhanced oxidized electrolyzed water.

According to the present invention, the obtained active oxygen/active nitrogen enhanced oxidized electrolyzed water has acidity, which can affect the normal physiological metabolism of cells and make them inactive. At the same time, the active oxygen/active nitrogen-enhanced oxidized electrolyzed water also has relatively high oxidizing properties, which can oxidatively damage cells. Reactive oxygen/active nitrogen enhanced oxidation electrolyzed water has active chlorine, active oxygen and active nitrogen, among which active oxygen/active nitrogen is conducive to oxidizing and destroying the extracellular polymer of bacterial biofilm, which can react with carbon-hydrogen bonds to make bacteria The cell wall becomes weak, which can destroy the intramolecular bonds of peptidoglycan, causing the cell wall to rupture, causing oxidative stress of the microbial cell membrane, changing the surface structure and chemical state of the bacteria, so that the active chlorine can smoothly penetrate into the cell interior, resulting in DNA decomposition, Protein disruption and damage to other internal components.

As a preferred solution, in step S1, the anode adopts a titanium-based iridium oxide composite electrode, and the cathode adopts a titanium plate electrode.

By controlling the distance between the cathode plate and the anode plate, the current size can be better adjusted. As a preferred solution, the distance between the cathode electrode plate and the anode electrode plate is 0.5-5 cm, preferably 1-3 cm.

As a preferred solution, in step S1, the homogeneous ion exchange membrane is a homogeneous cation exchange membrane or a homogeneous anion exchange membrane. A cation exchange membrane is preferably used.

If the concentration of sodium chloride is too low, a certain concentration of available chlorine cannot be produced by electrolysis; if the concentration of sodium chloride is too high, the residual chloride ion content in the solution after electrolysis is too high. By controlling the concentration of the sodium chloride aqueous solution, the influence of the sodium chloride concentration on the available chlorine content is avoided. As a preferred solution, in step S1, the concentration of the sodium chloride aqueous solution is 0.5-5 g/L, more preferably 1-3 g/L.

As a preferred solution, in step S1, the constant current density of the constant current electrolysis is 1-10 mA/cm ² , more preferably 2-7 mA/cm ² .

As a preferred solution, in step S1, the electrolysis time is 1-60 min, more preferably 10-30 min.

Under the condition that the constant current density of constant current electrolysis and the above-mentioned electrolysis time are satisfied, the content of available chlorine can be more effectively affected, and the available chlorine content of 10-200 mg/L can be generated.

According to the present invention, the acidic oxidized electrolyzed water obtained by the above method satisfies the redox potential value ≥1100mV.

According to the present invention, the acidic oxidized electrolyzed water obtained by the above method satisfies the effective chlorine content of 10-200 mg/L.

According to the present invention, the acidic oxidized electrolyzed water obtained by the above method satisfies the pH value≤3.00.

As a preferred solution, in step S2, the volume ratio of nitrogen and oxygen in the carrier gas is (2-4):1.

Plasma discharge can only be performed when the carrier gas pressure is within a certain range. If the carrier gas pressure is too low, plasma cannot be generated; if the carrier gas pressure is too high, the discharge efficiency will be affected, and a large amount of carrier gas cannot be ionized. As a preferred solution, in step S2, the pressure of the carrier gas is 0.1-0.3 MPa, more preferably 0.12-0.16 MPa.

The discharge current affects the plasma generation efficiency. When the discharge current is too low, sufficient plasma cannot be generated. The discharge current is too high and the energy consumption increases. As a preferred solution, in step S2, the current of the glow discharge is 2.00-4.00A, more preferably 3.00-3.50A.

The activation time will affect the content of reactive oxygen species and reactive nitrogen species. As a preferred solution, in step S2, the activation time is 0.5-60 min, more preferably 1-20 min.

As a preferred solution, in step S2, the activation volume is 50-200 mL.

As a preferred solution, in step S2, the low-temperature plasma activation methods can be two methods: water discharge and underwater discharge.

As a preferred solution, in step S2, the distance between the plasma jet probe and the liquid is 50-80 mm (water activation) or 10-20 mm (underwater activation).

The second aspect of the present invention provides active oxygen/active nitrogen enhanced oxidized electrolyzed water obtained by the above-mentioned preparation method of active oxygen/active nitrogen enhanced oxidized electrolyzed water.

According to the present invention, the pH value of the active oxygen/active nitrogen enhanced oxidized electrolyzed water obtained by the above method is less than or equal to 2.50. A decrease in pH is beneficial to increase the bactericidal activity. After activation, the pH value is further lowered.

According to the present invention, the active chlorine content of the active oxygen/active nitrogen enhanced oxidized electrolyzed water obtained by the above method is 10-200 mg/L.

According to the present invention, the active oxygen content of the active oxygen/active nitrogen enhanced oxidized electrolyzed water obtained by the above method is 10-200 mg/L.

According to the present invention, the active oxygen/active nitrogen enhanced oxidized electrolyzed water obtained by the above method has an active nitrogen content of 10-200 mg/L.

The third aspect of the present invention provides the application of the above-mentioned active oxygen/active nitrogen enhanced oxidized electrolyzed water in the preparation of a bactericide.

The technical scheme of the present invention has the following beneficial effects:

(1) The method of the present invention is used to prepare an active oxygen/active nitrogen-enhanced oxidized electrolyzed water high-efficiency bactericide rich in active chlorine, active oxygen and active nitrogen, which has high bactericidal efficiency for pathogenic microorganism membranes.

(2) The active oxygen/active nitrogen enhanced oxidized electrolyzed water of the present invention has the characteristics of safety, environmental protection and non-toxicity, does not cause environmental pollution problems, and has wide applicability.

Other features and advantages of the present invention will be described in detail in the detailed description that follows.

Detailed ways

Preferred embodiments of the present invention will be described in more detail below. While the preferred embodiments of the present invention are described below, it should be understood that the present invention may be embodied in various forms and should not be limited by the embodiments set forth herein. Rather, these embodiments are provided so that this disclosure will be thorough and complete, and will fully convey the scope of the present invention to those skilled in the art.

In the following examples and comparative examples, in the ionic membrane electrolyzer, the cation exchange membrane used was purchased from Hangzhou Lvhe Environmental Protection Technology Co., Ltd., the model is HoCEM Grion 0011T; the anode adopts titanium-based iridium oxide alloy electrode, and its preparation method See the patent title of a preparation method of a platinum-iridium oxide alloy electrode, and the patent number is Example 1 of the invention patent of ZL 201410452379.X, wherein the molar ratio of platinum-iridium is 1:1. The plasma generator adopts the low temperature plasma machine produced by Shenzhen Hongcheng Electronic Technology Co., Ltd.

In each embodiment of the present invention, the entire electrolysis reaction is performed dynamically.

Example 1

S1: Preparation of oxidized electrolyzed water: In the ion-exchange membrane electrolyzer, the ion-exchange membrane is used to divide the ion-exchange membrane into two parts, the cathode area and the anode area. The anode uses titanium-based iridium oxide alloy electrodes, and the cathode uses titanium plate electrodes. The distance between the cathode electrode plate and the anode electrode plate is 2 cm, and the effective area of the electrode is 75 cm ² . 400 mL of NaCl solution with a concentration of 1 g/L was added as the electrolyte in the cathode area and the anode area, respectively. During the electrolysis process, electrolysis was carried out at a current density of 4.5 mA/cm ² for 3 min, and oxidized electrolyzed water was obtained in the anode area. After testing, the pH value of the oxidized electrolyzed water was 2.95, the redox potential value was 1125mV, and the available chlorine was 18.06mg/L.

S2: Preparation of active oxygen/active nitrogen-enhanced oxidized electrolyzed water: place the oxidized electrolyzed water prepared in step S1 in an activation chamber, and place the plasma generator spray head above or below the oxidized electrolyzed aqueous solution; The carrier gas of nitrogen and oxygen is the excitation gas source, and a low-temperature plasma is generated by glow discharge; it is sprayed into the oxidized electrolyzed water, and chemically reacts with the oxidized electrolyzed water to obtain a plasma rich in active chlorine, active oxygen and active nitrogen. The active oxygen/active nitrogen enhanced oxidized electrolyzed water.

The activation conditions are: the gas source type is a 4:1 mixture of nitrogen and oxygen, the carrier gas pressure is about 0.13MPa, the discharge current is 3.04A, the activation volume is 50mL, and the distance between the plasma jet probe and the liquid is 80mm (on water). Activation time 3min. The pH value of the prepared active oxygen/active nitrogen enhanced oxidized electrolyzed water is 2.41; the redox potential value is 609mV; the hydrogen peroxide concentration is 47.66mg/L; the nitrate ion concentration is 71.38mg/L; the nitrite ion The concentration is 48.57mg/L.

Test Example 1: Bactericidal effect of active oxygen/active nitrogen enhanced oxidized electrolyzed water

Take the Bacillus subtilis biofilm stainless steel sheet cultured for more than 7 days and rinse it with PBS solution for 2 to 3 times. Put the stainless steel sheet into 10 mL of active oxygen/active nitrogen enhanced oxidized electrolyzed water for 10s. The stainless steel sheet was taken out and placed in a PE tube containing 10 mL of 0.5% sodium thiosulfate solution (neutralizer) for 10 min. The stainless steel sheet was taken and moved into 10 mL of sterile saline, and ultrasonicated for 15 min (100W, 25°C). After doing gradient dilution, select a suitable gradient for plate culture, and count after culturing at 37°C for 24 hours. In the control group, take the Bacillus subtilis biofilm stainless steel sheet cultured for more than 7 days, rinse with PBS solution for 2 to 3 times, put the stainless steel sheet into 10 mL of sterilized physiological saline, ultrasonicate for 15 min (100 W, 25 °C), and select after gradient dilution. Appropriate gradients were used for plate culture, and the cells were counted after culturing at 37°C for 24 hours. The log kill was calculated to be 1.76 log ₁₀ CFU.

Example 2

S1: Preparation of oxidized electrolyzed water: In the ion-exchange membrane electrolyzer, the ion-exchange membrane is used to divide the ion-exchange membrane into two parts, the cathode area and the anode area. The anode uses titanium-based iridium oxide alloy electrodes, and the cathode uses titanium plate electrodes. The distance between the cathode electrode plate and the anode electrode plate is 4 cm, and the effective area of the electrode is 75 cm ² . 400 mL of NaCl solution with a concentration of 2 g/L was added as the electrolyte in the cathode area and the anode area, respectively. During the electrolysis process, electrolysis was performed at a current density of 4.5 mA/cm ² for 15 min, and oxidized electrolyzed water was obtained in the anode area. After testing, the pH value of the oxidized electrolyzed water was 2.69, the redox potential value was 1171mV, and the available chlorine was 84.04mg/L.

S2: Preparation of active oxygen/active nitrogen-enhanced oxidized electrolyzed water: place the oxidized electrolyzed water prepared in step S1 in an activation chamber, and place the plasma generator spray head above or below the oxidized electrolyzed aqueous solution; The carrier gas of nitrogen and oxygen is the excitation gas source, and a low-temperature plasma is generated by glow discharge; it is sprayed into the oxidized electrolyzed water, and chemically reacts with the oxidized electrolyzed water to obtain a plasma rich in active chlorine, active oxygen and active nitrogen. The active oxygen/active nitrogen enhanced oxidized electrolyzed water.

The activation conditions are: the gas source type is compressed air, the carrier gas pressure is about 0.15MPa, the discharge current is 3.04A, the activation volume is 200mL, the distance between the plasma jet probe and the liquid is 80mm (on water), and the activation time is 10min. The pH value of the prepared active oxygen/active nitrogen enhanced oxidized electrolyzed water is 2.22; the redox potential value is 653mV; the hydrogen peroxide concentration is 22.39mg/L; the nitrate ion concentration is 53.93mg/L; the nitrite ion The concentration is 13.76mg/L.

Test Example 2: Bactericidal effect of active oxygen/active nitrogen enhanced oxidized electrolyzed water

The sterilization process was the same as in Example 1, and the calculated log kill value was expressed as 1.50 log ₁₀ CFU.

Example 3

S1: Preparation of oxidized electrolyzed water: In the ion-exchange membrane electrolyzer, the ion-exchange membrane is used to divide the ion-exchange membrane into two parts, the cathode area and the anode area. The anode uses titanium-based iridium oxide alloy electrodes, and the cathode uses titanium plate electrodes. The distance between the cathode electrode plate and the anode electrode plate is 2 cm, and the effective area of the electrode is 75 cm ² . 400 mL of NaCl solution with a concentration of 5 g/L was added as the electrolyte in the cathode area and the anode area, respectively. During the electrolysis process, electrolysis was carried out at a current density of 7.0 mA/cm ² for 30 min, and oxidized electrolyzed water was obtained in the anode area. After testing, the pH value of the oxidized electrolyzed water was 2.33, the redox potential value was 1192mV, and the available chlorine was 145.83mg/L.

S2: Preparation of active oxygen/active nitrogen-enhanced oxidized electrolyzed water: place the oxidized electrolyzed water prepared in step S1 in an activation chamber, and place the plasma generator spray head above or below the oxidized electrolyzed aqueous solution; The carrier gas of nitrogen and oxygen is the excitation gas source, and a low-temperature plasma is generated by glow discharge; it is sprayed into the oxidized electrolyzed water, and chemically reacts with the oxidized electrolyzed water to obtain a plasma rich in active chlorine, active oxygen and active nitrogen. The active oxygen/active nitrogen enhanced oxidized electrolyzed water.

The activation conditions are: the gas source type is a 2:1 mixture of nitrogen and oxygen, the carrier gas pressure is about 0.20MPa, the current is 3.50A, the activation volume is 200mL, the distance between the plasma jet probe and the liquid is 50mm (on water), and the activation Time 20min. The pH value of the prepared active oxygen/active nitrogen enhanced oxidized electrolyzed water is 2.41; the redox potential value is 637mV; the hydrogen peroxide concentration is 116.16mg/L; the nitrate ion concentration is 140.79mg/L; The concentration is 134.93mg/L.

Test Example 3: Bactericidal effect of active oxygen/active nitrogen enhanced oxidized electrolyzed water

The sterilization process was the same as in Example 1, and the calculated log kill value was expressed as 2.87 log ₁₀ CFU.

Example 4

S1: Preparation of oxidized electrolyzed water: In the ion-exchange membrane electrolyzer, the ion-exchange membrane is used to divide the ion-exchange membrane into two parts, the cathode area and the anode area. The anode uses titanium-based iridium oxide alloy electrodes, and the cathode uses titanium plate electrodes. The distance between the cathode electrode plate and the anode electrode plate is 2 cm, and the effective area of the electrode is 75 cm ² . 400 mL of NaCl solution with a concentration of 1 g/L was added as the electrolyte in the cathode area and the anode area, respectively. During the electrolysis process, electrolysis was carried out at a current density of 4.5 mA/cm ² for 8 min, and oxidized electrolyzed water was obtained in the anode area. After testing, the pH value of the oxidized electrolyzed water is 2.83, the redox potential value is 1152mV, and the available chlorine is 45.83mg/L.

S2: Preparation of active oxygen/active nitrogen-enhanced oxidized electrolyzed water: place the oxidized electrolyzed water prepared in step S1 in an activation chamber, and place the plasma generator spray head above or below the oxidized electrolyzed aqueous solution; The carrier gas of nitrogen and oxygen is the excitation gas source, and a low-temperature plasma is generated by glow discharge; it is sprayed into the oxidized electrolyzed water, and chemically reacts with the oxidized electrolyzed water to obtain a plasma rich in active chlorine, active oxygen and active nitrogen. The active oxygen/active nitrogen enhanced oxidized electrolyzed water.

The activation conditions are: the gas source type is a 4:1 mixture of nitrogen and oxygen, the carrier gas pressure is about 0.13MPa, the current is 3.04A, the activation volume is 200mL, and the distance between the plasma jet probe and the liquid is 20mm (under water). Activation time 3min. The pH value of the prepared active oxygen/active nitrogen enhanced oxidized electrolyzed water is 2.41; the redox potential value is 637mV; the hydrogen peroxide concentration is 16.16mg/L; the nitrate ion concentration is 40.79mg/L; The concentration is 34.93mg/L.

Test Example 4: Bactericidal effect of active oxygen/active nitrogen enhanced oxidized electrolyzed water

The sterilization process was the same as in Example 1, and the calculated log kill value was expressed as 1.48 log ₁₀ CFU.

Example 5

S1: Preparation of oxidized electrolyzed water: In the ion-exchange membrane electrolyzer, the ion-exchange membrane is used to divide the ion-exchange membrane into two parts, the cathode area and the anode area. The anode uses titanium-based iridium oxide alloy electrodes, and the cathode uses titanium plate electrodes. The distance between the cathode electrode plate and the anode electrode plate is 2 cm, and the effective area of the electrode is 75 cm ² . 400 mL of NaCl solution with a concentration of 1 g/L was added as the electrolyte in the cathode area and the anode area, respectively. During the electrolysis process, electrolysis was carried out at a current density of 4.5 mA/cm ² for 3 min, and oxidized electrolyzed water was obtained in the anode area. After testing, the pH value of the oxidized electrolyzed water was 2.95, the redox potential value was 1125mV, and the available chlorine was 18.06mg/L.

S2: Preparation of active oxygen/active nitrogen-enhanced oxidized electrolyzed water: place the oxidized electrolyzed water prepared in step S1 in an activation chamber, and place the plasma generator spray head above or below the oxidized electrolyzed aqueous solution; The carrier gas of nitrogen and oxygen is the excitation gas source, and a low-temperature plasma is generated by glow discharge; it is sprayed into the oxidized electrolyzed water, and chemically reacts with the oxidized electrolyzed water to obtain a plasma rich in active chlorine, active oxygen and active nitrogen. The active oxygen/active nitrogen enhanced oxidized electrolyzed water.

The activation conditions are: the gas source type is a 4:1 mixture of nitrogen and oxygen, the carrier gas pressure is about 0.13MPa, the discharge current is 3.04A, the activation volume is 50mL, and the distance between the plasma jet probe and the liquid is 80mm (on water). Activation time 3min. The pH value of the prepared active oxygen/active nitrogen enhanced oxidized electrolyzed water is 2.41; the redox potential value is 609mV; the hydrogen peroxide concentration is 47.66mg/L; the nitrate ion concentration is 71.38mg/L; the nitrite ion The concentration is 48.57mg/L.

Test Example 5: Bactericidal effect of active oxygen/active nitrogen enhanced oxidized electrolyzed water

Take 1 ml of Bacillus subtilis bacterial suspension with a colony concentration of 10 ⁵ CFU/mL, add 9 mL of active oxygen/active nitrogen enhanced oxidized electrolyzed water, and sterilize for 10 s. Then, 1 mL of the above mixed solution was pipetted, and 9 mL of 0.5% sodium thiosulfate solution was added for neutralization for 10 min. Take 0.1mL for plate counting culture; culture at 36±1℃ for 24h. The log kill was calculated to be 2.03 log ₁₀ CFU.

Comparative Example 1

In the ion-exchange membrane electrolyzer, the ion-exchange membrane is used to divide the ion-exchange membrane into two parts: the cathode area and the anode area. The anode adopts titanium-based iridium oxide alloy electrode, the cathode adopts titanium plate electrode, and the difference between cathode plate and anode plate is used. The spacing is 2 cm, and the electrode effective area is 75 cm ² . 400 mL of NaCl solution with a concentration of 1 g/L was added to the cathode area and the anode area as the electrolyte. During the electrolysis process, electrolysis was performed at a current density of 4.5 mA/cm ² for 5 min, and oxidized electrolyzed water was obtained in the anode area. After testing, the pH value of the oxidized electrolyzed water was 2.93, the redox potential value was 1129mV, and the available chlorine was 19.61mg/L.

Test Example 6: Bactericidal effect of oxidized electrolyzed water

The sterilization process was the same as that of Example 1, and the calculated bacteriostatic rate was 38.44%, which was expressed as 0.21 log ₁₀ CFU by the log kill value.

Various embodiments of the present invention have been described above, and the foregoing descriptions are exemplary, not exhaustive, and not limiting of the disclosed embodiments. Numerous modifications and variations will be apparent to those of ordinary skill in the art without departing from the scope and spirit of the described embodiments.

Claims (10)

1. A method for preparing active oxygen/active nitrogen enhanced oxidized electrolyzed water is characterized by comprising the following steps:

s1: preparation of oxidized electrolyzed water: in an ion exchange membrane electrolytic cell, a homogeneous ion exchange membrane is adopted to divide the electrolytic cell into a cathode area and an anode area; respectively adding sodium chloride aqueous solution as electrolyte in a cathode area and an anode area, and performing constant current electrolysis to obtain acidic oxidation electrolyzed water in the anode area and alkaline reduction electrolyzed water in the cathode area;

s2: preparing active oxygen/active nitrogen enhanced oxidation electrolyzed water: placing the oxidized electrolyzed water prepared in the step S1 in an activation chamber, and placing a plasma generator spray head above or below the oxidized electrolyzed water solution; taking a carrier gas containing nitrogen and oxygen as an excitation gas source, and generating low-temperature plasma in a glow discharge mode; and the active oxygen/active nitrogen enhanced oxidized electrolyzed water rich in active chlorine, active oxygen and active nitrogen is obtained by injecting the active oxygen/active nitrogen enhanced oxidized electrolyzed water into oxidized electrolyzed water and carrying out chemical reaction with the oxidized electrolyzed water.

2. The method of producing activated oxygen/activated nitrogen enhanced oxygenated electrolyzed water as claimed in claim 1, wherein in step S1,

the anode adopts a titanium-based iridium oxide composite electrode, and the cathode adopts a titanium plate electrode;

the distance between the cathode plate and the anode plate is 0.5-5 cm;

the homogeneous phase ion exchange membrane is a homogeneous phase cation exchange membrane or a homogeneous phase anion exchange membrane.

3. The method for producing activated oxygen/activated nitrogen enhanced oxidized electrolyzed water according to claim 1, wherein in step S1, the concentration of the aqueous sodium chloride solution is 0.5 to 5 g/L.

4. The method for producing activated oxygen/activated nitrogen enhanced oxidized electrolyzed water according to claim 1, wherein in step S1, the constant current density of constant current electrolysis is 1 to 10mA/cm²The electrolysis time is 1-60 min.

5. The method for producing activated oxygen/activated nitrogen enhanced oxidized electrolyzed water according to claim 1, wherein in step S1, the obtained acidic oxidized electrolyzed water satisfies:

the oxidation-reduction potential value is more than or equal to 1100 mV;

the content of available chlorine is 10-200 mg/L;

the pH value is less than or equal to 3.00.

6. The method for producing activated oxygen/activated nitrogen enhanced oxidized electrolyzed water according to claim 1, wherein in step S2,

the volume ratio of nitrogen to oxygen in the carrier gas is (2-4): 1;

the pressure of the carrier gas is 0.1-0.3 MPa.

7. The method for producing activated oxygen/activated nitrogen enhanced oxidized electrolyzed water according to claim 1, wherein in step S2,

the current of glow discharge is 2.00-4.00A;

the activation time is 0.5-60 min.

8. The active oxygen/active nitrogen-enhanced oxidized electrolyzed water obtained by the method for producing active oxygen/active nitrogen-enhanced oxidized electrolyzed water according to any one of claims 1 to 7.

9. The active oxygen/active nitrogen enhanced oxygenated electrolyzed water of claim 8,

the pH value of the active oxygen/active nitrogen enhanced oxidation electrolyzed water is less than or equal to 2.50;

the active chlorine content of the active oxygen/active nitrogen enhanced oxidized electrolyzed water is 10-200 mg/L;

the active oxygen content of the active oxygen/active nitrogen enhanced oxidized electrolyzed water is 10-200 mg/L;

the active nitrogen content of the active oxygen/active nitrogen enhanced oxidized electrolyzed water is 10-200 mg/L.

10. Use of the active oxygen/active nitrogen enhanced oxidized electrolyzed water according to claim 8 or 9 for producing a bactericide.