CN114469843A

CN114469843A - Composition with soothing and repairing effects and preparation method and application thereof

Info

Publication number: CN114469843A
Application number: CN202210190014.9A
Authority: CN
Inventors: 孔秋婵; 夏高辉; 毛善巧; 陈庆生
Original assignee: Guangzhou Huanya Cosmetic Science and Technology Co Ltd
Current assignee: Guangzhou Huanya Cosmetic Science and Technology Co Ltd
Priority date: 2022-02-28
Filing date: 2022-02-28
Publication date: 2022-05-13

Abstract

The invention belongs to the technical field of cosmetics, and discloses a composition with a soothing and repairing effect, and a preparation method and application thereof. The composition comprises the following components: synbiotics, a stimulus-inhibiting component, a sebum-mimicking component, and a damage-repairing component; the synbiotic is at least two of trehalose, beta-glucan, lactobacillus fermentation product or schizosaccharomyces fermentation product lysate; the irritation inhibiting component is at least one of hydroxyphenylpropionamide benzoic acid, bisabolol or ginger root extract; the damage repairing component is at least one of tetrahydropyrimidine carboxylic acid, Inonotus obliquus extract or madecassoside. The composition has good skin-soothing and repairing effects, can balance the microecology of the skin, utilizes microbial competition to kill harmful bacteria, effectively relieves acne and inflammation, increases the moisture content in the horny layer, strengthens the skin barrier and enhances the skin tolerance. The preparation method of the composition is simple and easy for industrial production.

Description

Composition with soothing and repairing effects and preparation method and application thereof

Technical Field

The invention belongs to the technical field of cosmetics, and relates to a composition with a soothing and repairing effect, and a preparation method and application thereof.

Background

Sensitive skin is a sub-healthy state of the skin and is mainly characterized in that the tolerance threshold of the skin is reduced, and the skin is easily stimulated by various internal and external factors to have uncomfortable feelings such as pruritus, burning, irritation, dryness or tightness, and occasionally accompanied by erythema, desquamation and the like. Factors responsible for skin sensitivity mainly include:

(1) drying and water shortage: after the moisture on the surface layer of the skin is lost, the skin can have the problems of roughness, pruritus, chapping, desquamation and the like; if the skin is lacked for a long time, the skin is cracked, so that the barrier is damaged, and the health of the skin is influenced;

(2) external pollution: the stimulation of external adverse environments such as ultraviolet rays, automobile exhaust, dust in air, microorganisms and the like easily causes pigment precipitation, water-oil imbalance and sensitive stimulation reaction in a short time; the collagen in the skin is easily excessively lost, the cells are damaged, the immunity of the skin is weakened, and the aging process of the skin is accelerated;

(3) poor skin care: the use of excessively cleaning skin and disinfecting products easily influences the balance of flora on the surface of the skin, excessive exfoliating easily causes damage to skin barriers, and long-term irregular use of hormone medicines causes dependence dermatitis;

(4) physiological reasons are as follows: the self-metabolism is reduced, the body immunity is reduced, the skin tolerance is reduced, skin cells are easy to be stimulated by the outside, and the phenomena of redness, fever, pruritus, stabbing pain, erythra and the like are generated.

In conclusion, the reason for skin sensitivity is the result of the interaction of various factors, and a soothing and repairing product which merely moisturizes or repairs the skin barrier cannot improve the skin sensitivity.

Therefore, there is a need to provide a cosmetic product having soothing and repairing effects on the above sensitive skin.

Disclosure of Invention

The present invention is directed to solving at least one of the problems of the prior art described above. Therefore, the composition with the effect of relieving and repairing sensitive skin is applied to cosmetics and has the effect of relieving and repairing the sensitive skin.

In a first aspect of the invention, there is provided a composition having soothing and repairing effects; the composition comprises the following components: synbiotics, a stimulus-inhibiting component, a sebum-mimicking component, and a damage-repairing component.

The synbiotic is at least two of trehalose, beta-glucan, a lactobacillus fermentation product or a split yeast fermentation product lysate.

The irritation inhibiting component is at least one of hydroxyphenylpropionamide benzoic acid, bisabolol or ginger root extract.

The sebum bionic component is at least one of ceramide, sterol derivative, phospholipid or vegetable oil.

The damage repairing component is at least one of tetrahydro-methyl pyrimidine carboxylic acid, Inonotus obliquus extract or madecassoside. The synbiotics are beneficial components for regulating the microecology of skin and consist of prebiotics and prebiotics. The prebiotics can promote the metabolism and proliferation of skin beneficial bacteria, and the prebiotics are probiotic metabolites or soluble factors released after thalli die and dissolve. Trehalose is not only a prebiotic, but also has strong moisturizing effect and physiological effects in various aspects such as sun protection, ultraviolet protection and the like, and can improve the environmental adaptability of skin in dry and cold environments.

The beta-glucan disclosed by the invention is used as a prebiotic, and also has the effects of improving the moisture-retaining capacity of the skin, resisting allergy and inflammation, improving the capacity of resisting external stimulation of the skin and having a certain anti-aging effect.

The lactobacillus fermentation product is a probiotic component prepared by using a mimicry technology, can preserve the complete cell wall structure of bacteria, regulate the microbial flora of skin, enhance the barrier function of the skin, relieve the skin, promote the skin to be healthy and balanced, and achieve the effect of recovering the self-defense capability of the skin; the lysate of the yeast fermentation product is a probiotic component obtained by inactivating and decomposing bifidobacterium, contains various beneficial skin care micromolecule components such as vitamin B group, mineral substances, amino acids and the like, has the activity of resisting immunosuppression, and can maintain the normal and stable state of skin.

The irritation inhibiting component can inhibit erythema, edema and pruritus caused by anaphylaxis, and achieves the effect of relieving. The hydroxyphenylpropionamide benzoic acid can inhibit histamine release of mast cells, prevent substance P released by nerve endings from being combined with NK-1 receptors of the mast cells and inhibit anaphylactic reaction; bisabolol is an inhibitor of IL-1 α, and ginger root extract is a PGE2 inhibitor, which can improve sensitivity causing erythema, edema, heat and pain, and enhance skin stability.

The sebum bionic component is a component which is soft, moist and protective in the simulated sebum membrane, so as to prevent skin from being dried and cracked, form a natural barrier of the skin and protect the health of the skin. The sterol derivative can maintain the softness, moisture and luster of skin, has mild permeability to skin, can promote the synthesis of collagen, and can delay skin aging; ceramide is the main part of intercellular matrix, has strong ability of associating water molecules, can enhance adhesion between keratinocytes, improve skin dryness, reduce desquamation of skin, and maintain skin barrier; the phospholipid is an important component of a cell membrane and is one of important components of a skin lipid membrane, the phospholipid can enhance the moisture retention capacity of the skin and promote the absorption of the skin to active components, and meanwhile, the phospholipid also has an emulsifying function and contributes to the stability of a system; the vegetable oil and fat not only can preserve moisture and moisten, but also the unsaturated fatty acid in the vegetable oil and fat has the functions of resisting oxidation, enhancing the skin immunity and preventing skin inflammation.

The damaged repairing component of the invention provides nutrients for basal cells of the skin, promotes the division of the basal cells of the skin, accelerates the formation of new cutin and finally can achieve the effects of repairing and thickening the cutin. The content of superoxide dismutase of the Inonotus obliquus is the first in the mushroom community, has strong anti-inflammatory and antioxidant components, and can repair cell injury caused by long-term skin inflammation; madecassoside is the major active triterpene component in centella asiatica and is used for treating wound repair and scar management; the tetrahydro-methyl pyrimidine carboxylic acid can stabilize the structure of enzyme protein in organisms, has a stable protection effect on cells in adverse environments, can protect neutrophil granulocytes, has a good anti-inflammatory effect, and can provide good moisturizing and sun-blocking functions.

According to some embodiments of the invention, the synbiotic comprises a prebiotic and a prebiotic.

According to some embodiments of the invention, the prebiotic in the synbiotic is a lactobacillus fermentation product and/or a saccharomyces bifidus fermentation product lysate.

According to some embodiments of the invention, the prebiotic in the synbiotic is trehalose and/or beta-glucan.

According to some embodiments of the invention, the weight ratio of prebiotics to prebiotics in the synbiotic is (1-5): 1.

According to some embodiments of the invention, the synbiotic is trehalose, beta-glucan, lactobacillus fermentation product, and saccharomyces bifidus fermentation product lysate.

According to some embodiments of the invention, the synbiotic is trehalose, beta-glucan, and a schizosaccharomyces fermentate lysate.

According to some embodiments of the invention, the synbiotic is trehalose, beta-glucan, and a lactobacillus fermentation product.

According to some embodiments of the invention, the synbiotic is trehalose and a lactobacillus fermentation product.

According to some embodiments of the invention, the synbiotic is β -glucan and a lactobacillus fermentation product.

According to some embodiments of the invention, the synbiotic is trehalose and a saccharomyces bifidus fermentation product lysate.

According to some embodiments of the invention, the synbiotic is a beta-glucan and a yeast schizosaccharomyces fermentate lysate.

According to some embodiments of the invention, the ceramide is at least one of ceramide NP, ceramide AP, or ceramide EOP.

According to some embodiments of the invention, the ceramide is ceramide NP.

According to some embodiments of the invention, the ceramide is ceramide NP, ceramide AP and ceramide EOP.

According to some embodiments of the invention, the sebum-mimicking component further comprises a sterol derivative, a phospholipid and a vegetable oil.

According to some embodiments of the invention, the sterol derivative is at least one of phytosterols, cholesterol, soyasterol, phytosterol/octyldodecanol lauroyl glutamate or cholesterol/lanosterol mixed ester of C10-30 acids.

According to some embodiments of the invention, the sterol derivatives include phytosterol/octyldodecanol lauroyl glutamate and cholesterol/lanosterol mixed ester of C10-30 acids.

According to some embodiments of the invention, the sterol derivatives include phytosterols, cholesterol, glyceollins, and mixed cholesterol/lanosterol C10-30 acid esters.

According to some embodiments of the invention, the irritation-inhibiting component is hydroxyphenylpropionamide benzoic acid.

According to some embodiments of the invention, the irritation-inhibiting component is bisabolol and ginger root extract.

According to some embodiments of the present invention, the weight ratio of bisabolol to ginger root extract in the irritation-inhibiting composition is (9-10): 1.

According to some embodiments of the present invention, the weight ratio of bisabolol to ginger root extract in the irritation-inhibiting component is (9.2-9.9): 1.

According to some embodiments of the invention, the damage repair component is a tetrahydromethyl pyrimidine carboxylic acid.

According to some embodiments of the invention, the damage-repairing component is inonotus obliquus extract and madecassoside.

According to some embodiments of the invention, the composition comprises the following components in parts by weight: 8-30 parts of synbiotic, 2-15 parts of stimulation inhibition component, 45-90 parts of sebum bionic component and 8-25 parts of damaged repair component.

According to some embodiments of the invention, the composition comprises the following components in parts by weight: 10-20 parts of synbiotics, 5-10 parts of stimulation inhibition components, 50-90 parts of sebum bionic components and 10-20 parts of damaged repair components.

According to some embodiments of the invention, the composition further comprises the following components: thickener, stearic acid and pH regulator.

According to some embodiments of the invention, the composition has a pH of 5.5 to 6.5.

According to some embodiments of the invention, the pH adjusting agent is aminomethyl propanol.

According to some embodiments of the invention, the thickener is EMT-10 and/or carbomer.

According to some embodiments of the invention, the thickener is EMT-10 or carbomer.

According to some embodiments of the invention, the composition further comprises a polyol, the polyol being 1, 2-pentanediol.

According to some embodiments of the invention, the composition comprises the following components in parts by weight: : 10-20 parts of synbiotic, 5-10 parts of stimulation inhibition component, 50-90 parts of sebum bionic component, 10-20 parts of damage repair component, 1-3 parts of thickening agent and 2-11 parts of stearic acid.

According to some embodiments of the invention, the composition comprises the following components in parts by weight: 10-20 parts of synbiotic, 5-10 parts of irritation inhibiting component, 50-90 parts of sebum bionic component, 10-20 parts of damage repairing component, 1-3 parts of thickening agent, 15-30 parts of polyhydric alcohol and 2-11 parts of stearic acid.

A second aspect of the invention provides a process for the preparation of the above composition.

The preparation method of the composition comprises the following steps: mixing the components to obtain the composition.

According to some embodiments of the invention, the method of preparing the composition comprises the steps of:

(1) mixing deionized water, thickener and trehalose, stirring, and heating to 85-90 deg.C; adding lysolecithin before emulsification to obtain a mixture A;

(2) mixing C10-30 acid cholesterol/lanosterol mixed ester, ceramide NP and stearic acid, stirring, and heating to 85-90 deg.C; adding sunflower seed oil and phytosterol/octyldodecanol lauroyl glutamate before emulsification to obtain a mixture B;

(3) slowly pumping the mixture B into the mixture A, and homogenizing and emulsifying at a high speed;

(4) pre-dissolving hydroxyphenylpropionamide benzoic acid in 1, 2-pentanediol, and cooling to 40-45 ℃; adding the mixture and the rest components (excluding aminomethyl propanol) into the mixture obtained in the step (3) and stirring and mixing;

(5) adding aminomethyl propanol, and adjusting pH of the mixture to 5.5-6.5.

(1) mixing deionized water and a thickening agent, stirring uniformly, and heating to 85-90 ℃; adding hydrogenated lecithin before emulsification to obtain a mixture A;

(2) mixing phytosterol, cholesterol, C10-30 acid cholesterol/lanosterol mixed ester, ceramide NP, ceramide AP, ceramide EOP and stearic acid, stirring, and heating to 85-90 deg.C; before emulsification, adding olive oil and shea butter to obtain a mixture B;

(4) adding the rest components (excluding aminomethyl propanol), stirring and mixing;

(5) adding aminomethyl propanol, and adjusting pH of the mixture to 5.5-6.5.

(1) mixing deionized water and a thickening agent, stirring uniformly, and heating to 85-90 ℃; adding lysolecithin before emulsification to obtain a mixture A;

(2) mixing phytosterol/octyldodecanol lauroyl glutamate, C10-30 cholesterol/lanosterol mixed ester, ceramide NP and stearic acid, stirring, and heating to 85-90 deg.C; adding shea butter resin before emulsification to obtain a mixture B;

(4) pre-dissolving hydroxyphenylpropionamide benzoic acid in 1, 2-pentanediol, and cooling to 40-45 ℃; then adding the mixture and the rest components (excluding aminomethyl propanol) into the mixture obtained in the step (3) to be stirred and mixed;

(5) adding aminomethyl propanol, and adjusting pH of the mixture to 5.5-6.5.

A third aspect of the invention provides the use of the above composition in the preparation of a cosmetic product.

According to some embodiments of the invention, the cosmetic is a soothing and repairing cosmetic.

According to some embodiments of the invention, the cosmetic is a cream, a serum, or a lotion.

According to some embodiments of the invention, 20% to 50% of the composition is added to the cosmetic product.

In a fourth aspect, the present invention provides a cosmetic product comprising the composition described above.

According to some embodiments of the invention, the cosmetic comprises 20% -50% of the composition.

According to some embodiments of the invention, the cosmetic is at least one of a cream, a serum, and/or a lotion.

According to some embodiments of the invention, the cosmetic is a cream comprising the following components: acrylamide dimethyl ammonium taurate/VP copolymer, glycerol, xanthan gum, sodium hyaluronate, 1, 3-propylene glycol, glycerol stearate, PEG-100 stearate, squalane, cyclopentadimethylsiloxane, 1, 2-hexanediol, p-hydroxyacetophenone, and combinations thereof.

According to some embodiments of the invention, the cosmetic comprises the following components: the composition comprises a phase A component, a phase B component, a phase C component and a phase D component, wherein the phase A component comprises acrylamide dimethyl ammonium taurate/VP copolymer, glycerol, xanthan gum, sodium hyaluronate and 1, 3-propylene glycol; the phase B component comprises glycerol stearate, PEG-100 stearate, squalane and cyclopentadimethylsiloxane; the phase C component comprises 1, 2-hexanediol and p-hydroxyacetophenone; the phase D component includes the compositions described above.

According to some embodiments of the present invention, the phase a component comprises, in parts by weight, 0.3 to 1 part of acrylamide dimethyl taurate/VP copolymer, 6 to 10 parts of glycerin, 0.05 to 0.2 part of xanthan gum, 0.05 to 0.2 part of sodium hyaluronate, 4 to 10 parts of 1, 3-propanediol; the phase B component comprises 0.5-2 parts of glycerol stearate, 0.5-2 parts of PEG-100 stearate, 3-8 parts of squalane and 4-8 parts of cyclopentasiloxane; the phase C component comprises 0.5-2 parts of 1, 2-hexanediol and 0.2-1 part of p-hydroxyacetophenone; the phase D component comprises 30 to 50 parts of the above composition.

According to some embodiments of the present invention, the phase a component comprises, in parts by weight, 0.5 parts of acrylamide dimethyl taurate/VP copolymer, 8 parts of glycerin, 0.1 parts of xanthan gum, 0.1 parts of sodium hyaluronate, 7 parts of 1, 3-propanediol; the phase B component comprises 1 part of glycerol stearate, 1 part of PEG-100 stearate, 5 parts of squalane and 6 parts of cyclopentylpentadimethyl siloxane; the phase C component comprises 1 part of 1, 2-hexanediol and 0.5 part of p-hydroxyacetophenone; the phase D component comprises 40 parts of the above composition.

The fifth aspect of the present invention provides a method for producing the above cosmetic.

According to some embodiments of the present invention, the method of preparing the cosmetic comprises the steps of:

(1) mixing the phase A components, and heating to dissolve to obtain a phase A mixture;

(2) mixing the phase B components, and heating to dissolve to obtain a phase B mixture;

(3) slowly adding the phase A mixture into the phase B mixture, homogenizing and cooling;

(4) cooling to 60-65 deg.C, adding phase C component, and stirring;

(5) cooling to below 40 deg.C, adding phase D component, and stirring.

(1) mixing the phase A components, and heating to 80-85 deg.C to dissolve to obtain phase A mixture;

(2) mixing the phase B components, and heating to 80-85 deg.C to dissolve to obtain phase B mixture;

(4) cooling to 60-65 deg.C, adding phase C component, and stirring;

(5) and (3) cooling to below 40 ℃, adding the phase D component, and uniformly stirring to obtain the cosmetic.

Therefore, the beneficial effects of the invention include:

1. the composition has good effects of relieving and repairing skin through the synergistic effect of synbiotic, an irritation inhibiting component, a sebum bionic component and a damaged repairing component, can balance skin micro-ecology, utilizes microbial competition to kill harmful bacteria, effectively relieves acne and inflammation, increases the moisture content in horny layer, reduces moisture loss, solves skin discomfort caused by dryness, quickly relieves skin problems of redness, swelling, itching, heating and the like caused by external irritation, is suitable for long-term use, strengthens skin barriers and enhances skin tolerance.

2. The preparation method of the composition is simple and easy for industrial production.

Detailed Description

In order to make the objects, technical solutions and advantages of the present invention more apparent, the technical solutions in the embodiments of the present invention will be clearly and completely described below.

The present invention will be further illustrated with reference to the following examples. The following examples are merely preferred embodiments of the present invention and should not be construed as limiting the scope of the invention as claimed.

The raw materials used in the following examples and a list of their suppliers are shown in table 1.

TABLE 1 list of materials and suppliers used in the examples

Example 1

A composition with soothing and repairing effects has a formula shown in Table 2. The preparation method of the composition comprises the following steps:

(5) adding aminomethyl propanol, and adjusting pH of the mixture to 5.5-6.5.

A soothing and repairing facial cream comprises, by weight, a phase A component (29.8 parts of deionized water, 0.5 parts of AVC (acrylamide dimethyl taurate/VP copolymer), 8 parts of glycerol, 0.1 part of xanthan gum, 0.1 part of sodium hyaluronate and 7 parts of 1, 3-propylene glycol), a phase B component (1 part of glycerol stearate, 1 part of PEG-100 stearate, 5 parts of squalane and 6 parts of cyclopentadecyldimethylsiloxane), a phase C component (1 part of 1, 2-hexanediol, 0.5 part of p-hydroxyacetophenone) and a phase D component (40 parts of the composition of the embodiment).

The preparation method of the face cream comprises the following steps:

(1) mixing the phase A components at 80-85 deg.C until they are dissolved to obtain phase A mixture;

(2) mixing the phase B components at 80-85 deg.C until dissolved;

(3) slowly adding the mixture obtained in the step (1), homogenizing for 15-30min, and cooling;

(4) cooling to 60-65 deg.C, adding phase C component, and stirring to disperse uniformly;

(5) cooling to below 40 deg.C, adding phase D component, stirring to disperse uniformly, filtering with 200 mesh sieve, and discharging.

Example 2

A composition with soothing and repairing effects has a formula shown in Table 2.

The preparation method of the composition comprises the following steps:

(5) adding aminomethyl propanol, and adjusting pH of the mixture to 5.5-6.5.

A cream with soothing and rejuvenating effects, which differs from the cream provided in example 1 in that phase D is 40 parts of the composition provided in this example. The preparation method of the face cream is the same as that of example 1.

Example 3

The preparation method of the composition comprises the following steps:

(5) adding aminomethyl propanol, and adjusting pH of the mixture to 5.5-6.5.

A soothing and rejuvenating facial cream having the composition as distinguished from the cream provided in example 1 in that phase D is 40 parts of the composition provided in this example. The preparation method of the face cream is the same as that of example 1.

Example 4

A composition with soothing and repairing effects has a formula shown in Table 2. The composition was prepared as in example 1.

Example 5

The composition was prepared as in example 2.

A soothing and rejuvenating facial cream having the composition as distinguished from the cream provided in example 1 in that phase D is 40 parts of the composition provided in this example.

The preparation method of the face cream is the same as that of example 1.

TABLE 2 Components and corresponding parts by weight of the compositions of examples 1-6

Example 6

The composition was prepared as in example 3.

Comparative example 1

A composition with soothing and repairing effects is shown in Table 3.

The composition was prepared as in example 1.

A cream having soothing and rejuvenating effects, the composition of which differs from the cream provided in example 1 in that phase D is 40 parts of the composition provided in this comparative example. The preparation method of the face cream is the same as that of example 1.

Table 3 respective components and parts by weight of the compositions provided in respective proportions

Comparative example 2

A composition with soothing and repairing effects is shown in Table 3. The composition was prepared as in example 1.

Comparative example 3

Comparative example 4

A composition with soothing and repairing effects has a formula shown in Table 3. The composition was prepared as in example 1.

Comparative example 5

Comparative example 6

Comparative example 7

Comparative example 8

Comparative example 9

A cream with soothing and repairing effects, the components of which are different from those of the cream provided in example 1, in that phase D is 40 parts of the composition provided in the comparative example. The preparation method of the face cream is the same as that of example 1.

Comparative example 10

Comparative example 11

Comparative example 12

Comparative example 13

Comparative example 14

Performance testing

1. Skin micro-ecological balance test

The experimental principle is as follows: experiments are carried out to compare and test the absorption and utilization rates of the skin flora to the specific plant protoplasm and the common glucose, thereby researching the consumption process of the specific probiotic nutrients by the skin flora.

The experimental method comprises the following steps: a part of the flora on the skin surface was taken and transplanted into each of the groups of examples 1-6 (using the compositions of examples 1-6 as experimental media), comparative examples 1-14 (using the compositions of comparative examples 1-14 as comparative media), and a control (using a control medium of commercially available glucose), and the amount of the glucose/plant puree nutrient remaining in each group, i.e., the consumption level, was measured after 48 hours.

The consumed water balance criteria are shown in table 5.

TABLE 5 Water consumption balance Standard

Consumption level (%)	Consumption state
		0-20	Without consumption
20-40	Poor degree of consumption
		40-60	Moderate degree of consumption
60-100	Up to total consumption

The results of the experiment are shown in Table 6.

Table 6 skin micro-ecological balance test results

According to tables 5-6, the probiotics and the pathogenic bacteria in the control group have higher absorption and utilization rate to the common glucose; by single factor outcome analysis: the synbiotics have the greatest effect on skin micro-ecology, the absorption and utilization rate of the synbiotics by probiotics is higher than that of pathogenic bacteria, wherein the lactobacillus fermentation product has the strongest inhibition effect on the pathogenic bacteria, the schizosaccharomyces fermentation product lysate has a certain inhibition effect, and the trehalose and beta-glucan have stronger promotion effect on the probiotics; the damaged repairing component has a certain effect on the skin micro-ecology; the absorption and utilization rate of the pathogenic bacteria on the sebum bionic components is slightly higher than that of the probiotics; the irritation inhibiting component has no effect on skin microecology. The embodiment group has higher absorption and utilization rate of nutrient components in the obtained composition through reasonable compounding of the components, has a synergistic effect, and is superior to a single component or a combination mode of any two or three components. The composition with the soothing and repairing effects provided by the invention can be utilized by probiotics on the surface of skin, can selectively protect the probiotics without being obviously metabolized by pathogenic bacteria, and is used as a biological inhibitor relative to the pathogenic bacteria.

2. Microbial competitive growth assay

The experimental principle is as follows: the effect and effect of specific compositions on the production of the flora in the presence of both probiotic and pathogenic bacteria was tested.

The experimental method comprises the following steps: the compositions of examples 1 to 4, the compositions of comparative examples 1 to 14 and the test medium (tryptone USP) without the added composition (control) were prepared at a concentration of 0.5%, respectively. The bacterial groups (mixed bacterial groups of Micrococcus clarkii and Staphylococcus aureus) are respectively cultured in each experimental medium, the number of Micrococcus clarkii after 0h, 6h and 24h culture (T0, T6 and T24) and the change rate of Micrococcus clarkii before and after 24h culture (T24/T0), the number of Staphylococcus aureus after 0h, 6h and 24h culture (T0, T6 and T24) and the change rate of Staphylococcus aureus before and after 24h culture (T24/T0) of each group are calculated. The test results are shown in table 7.

TABLE 7 microbial Competition test results

According to table 7, the compositions of examples 1 to 6 and comparative examples 1 to 14 all exhibited different degrees of promotion or inhibition of probiotics or pathogenic bacteria after 24h of cultivation. According to the single-factor experimental comparative examples 11-14, the composition has the most remarkable effects of promoting probiotics and inhibiting pathogenic bacteria, and shows that the nutrients in the composition can promote the growth of the probiotics, the growth of the pathogenic bacteria can not be promoted, but the number of the pathogenic bacteria is remarkably reduced due to lack of food and nutrition, so that the probiotics are selectively utilized by the probiotics, and the pathogenic bacteria are inhibited, wherein the lactobacillus fermentation product has the strongest effect of inhibiting the pathogenic bacteria; the stimulus-inhibiting ingredient has no effect on both probiotics and pathogenic bacteria; the damaged repairing components have certain promotion on probiotics and inhibition on pathogenic bacteria, but the effect is weak; the sebum bionic component has certain function of promoting the growth of pathogenic bacteria. Compared with comparative examples 1-14, the probiotic bacteria of example 1 are increased in number and the pathogenic bacteria are reduced in number, which shows that the components in the example are matched with each other, have synergistic effect and are better than a single component or a combination mode of any two or three components.

3. Test of moisturizing Effect

The experimental method comprises the following steps: 25 skin sensitive subjects aged 19-36 years were selected, the cream products of examples 1-6 and comparative examples 1-14 were used once a day at night, and the moisture content of the face was tested 20min after the subjects cleaned their face on days 0, 7 and 14 at 20 ± 2 ℃ and humidity (50 ± 10)% to evaluate the skin rejuvenation efficacy of the product. Each parameter was measured 3 times in succession and averaged to reduce measurement error.

Judging indexes: as shown in table 8.

Table 8 evaluation index: rate of change of moisture content

Dosage forms	Not reaching the standard	Reach the standard	Is excellent in
				Aqueous preparation	≤20％	20-40％	≥40％

The test results are shown in table 9. Wherein D is₀Facial water content measured on day 0; d₇Facial water content measured on day 7; d₁₄Facial water content measured on day 14.

TABLE 9 rate of change of moisture content

As can be seen from the data in Table 9, the creams of examples 1-6 and comparative examples 1-14 all had different degrees of effects on the moisture content of the skin compared to the control group; according to the single-factor experimental comparative examples 11-14, the bionic sebum component has the largest influence on the moisture content of the skin, and the moisture retention effect is the best; synbiotic and damaged repair ingredients have a certain effect on skin moisture; the stimulus suppressing component has no effect. In the bionic sebum component, the higher the ceramide and C10-30 acid cholesterol/lanosterol mixed ester is, the stronger the water holding capacity of the product is. Compared with comparative examples 1-14, example 1 has a better moisturizing effect, which shows that the components in the examples are matched with each other, have a synergistic effect, and are better than a single component or a combination mode of any two or three components.

4. Test of fading effect

The purpose of the experiment is as follows: an acute skin barrier injury model is established by a tape continuous pasting method, and the Erythema Index (EI) of the area is tested to judge whether the product has the function of repairing the skin barrier.

The test method comprises the following steps: selecting 25 skin sensitive subjects with age of 19-36 years, respectively using the cream products of examples 1-6 and comparative examples 1-14, selecting and marking a piece of skin with the size of 2cm multiplied by 2cm at the equivalent position of the two forearms of the subject, randomly dividing the two test areas into a natural repair area and a sample intervention area after establishing an acute skin barrier damage model, and selecting an undamaged area with the equivalent size at the adjacent position of the natural repair area as a blank control area. The natural repair area and the blank control area do not use products, and the sample intervention area uses corresponding products. And (3) testing the red pigment conditions of the natural restoration area, the sample intervention area and the blank control area within 30min by using a melanin index and erythema index measuring instrument.

And (3) judging standard: and judging the state of the barrier repair process for recovering the damaged skin according to the erythema index, wherein the higher the repair rate is, the better the red fading effect of the product is.

The results of the red fading test are shown in table 10.

TABLE 10 results of the discoloration test

According to table 10, the creams of examples 1 to 6 have a remarkable immediate repairing effect on damaged skin, showing red fading and soothing in a short time, compared to comparative examples 1 to 14 and natural repair zones. From the test results of the single-factor experimental comparative examples 11-14, the irritation-inhibiting component has obvious effects of fading red and relieving, the sebum-simulating component has certain effects of fading red and relieving, the synbiotic and the damaged repairing component have no instant repairing effect, and part of the comparative example cream has the effect of relieving skin fading instead, so that adverse effects appear. The components in the embodiment are matched with each other, have synergistic effect, are superior to a single component or a combination mode of any two or three components, and the red fading effect of the hydroxyphenyl propionamide benzoic acid is superior to that of the bisabolol.

5. Evaluation test for lactic acid stimulation efficacy on face

This test is used to evaluate the skin barrier repair efficacy of the product.

The experimental method comprises the following steps: selecting 40 skin sensitive subjects, age 19-36 years, performing test at nasolabial sulcus of the subjects, dripping 50uL of 10% lactic acid solution on a single-layer filter paper with diameter of 0.8cm, respectively inquiring the subjects for stabbing pain at 3min, 4min and 5min, grading according to 4-point method (0 is no stabbing pain, 1 is mild stabbing pain, 2 is moderate stabbing pain and 3 is severe stabbing pain), and recording the grading of the volunteers as the lactic acid stimulation grading (D)₀). The product application was carried out as required 2 times a day (examples 1-6 and comparative examples 1-14 creams were used, respectively) for 28 consecutive days and for the 14 th day of the test (D)₁₄) And day 28 (D)₂₈) And (6) performing return visit.

The results of the experiment are shown in Table 11.

TABLE 11 lactic acid test results

According to table 11, the creams of examples 1 to 6 and comparative examples 1 to 14 have certain repairing effect on skin barrier, can strengthen skin, enhance skin resistance and relieve response to external stimulation, and the effect of damaged repairing components on enhancing skin barrier is most obvious from single-factor experimental comparative examples 11 to 14; the synbiotic and sebum bionic components have a certain repairing effect in 14 days; although the irritation-suppressing component has immediate effects of fading red and soothing, the long-acting repairing effect is not good. The components in the embodiment are mutually matched, have synergistic action and are superior to a single component or a combination mode of any two or three components, and the combination of the inonotus obliquus extract and the madecassoside is superior to the repairing effect of the tetrahydro-methylpyrimidine carboxylic acid.

Therefore, the reasonable combination of the synbiotic, the stimulation and inhibition component, the sebum bionic component and the damaged repair component has good relieving and repairing effects, can improve the skin problem from multiple dimensions, can balance the skin micro-ecology, does not use a bactericide but uses a microorganism competition mode to kill harmful bacteria, and can effectively relieve acne and inflammation; can increase the water content of the horny layer, reduce the loss of external water of the horny layer of the skin and solve the skin discomfort caused by dryness; skin problems such as redness, swelling, itching, fever and the like caused by external stimulation can be quickly relieved without using hormone components; has effects of strengthening skin barrier and enhancing skin tolerance after long-term use. The combination can be reasonably adjusted, and the problem of various sensitive muscles can be solved in a targeted manner.

Finally, it should be noted that the above embodiment modes are only used for illustrating the technical solutions of the present application and are not limited, and although the present application is described in detail with reference to the above preferred embodiments, it should be understood by those skilled in the art that modifications or equivalent substitutions can be made on the technical solutions of the present application without departing from the spirit and scope of the technical solutions of the present application.

Claims

1. A composition comprising the following components: synbiotics, a stimulus-inhibiting component, a sebum-mimicking component, and a damage-repairing component;

the synbiotic is at least two of trehalose, beta-glucan, a lactobacillus fermentation product or a schizosaccharomyces fermentation product lysate;

the irritation-inhibiting component is at least one of hydroxyphenylpropionamidobenzoic acid, bisabolol or ginger root extract;

the damage repairing component is at least one of tetrahydro-methyl pyrimidine carboxylic acid, Inonotus obliquus extract or madecassoside.

2. The composition of claim 1,

the sebum bionic component is at least one of ceramide, sterol derivative, phospholipid or vegetable oil;

the ceramide is at least one of ceramide NP, ceramide AP or ceramide EOP.

3. The composition of claim 1,

the sterol derivative is at least one of phytosterol, cholesterol, soyasterol, phytosterol/octyldodecanol lauroyl glutamate or C10-30 acid cholesterol/lanosterol mixed ester;

the phospholipid is at least one of lecithin, hydrogenated lecithin or lysolecithin;

the vegetable oil is at least one of olive fruit oil, sea buckthorn fruit oil, shea butter, corn germ oil, linseed oil or sunflower seed oil.

4. The composition according to claim 1, characterized by comprising the following components in parts by weight:

8-30 parts of synbiotic, 2-15 parts of stimulation inhibition component, 45-90 parts of sebum bionic component and 8-25 parts of damaged repair component.

5. The composition of claim 1, wherein the composition has a pH of 5.5 to 6.5.

6. A process for the preparation of a composition according to any one of claims 1 to 5, characterized in that it comprises the following steps:

mixing the components to obtain the composition.

7. Use of a composition according to any one of claims 1 to 5 for the preparation of a cosmetic product.

8. A cosmetic comprising the composition according to any one of claims 1 to 5.

9. The cosmetic according to claim 8, characterized in that it comprises the following components: the composition comprises a phase A component, a phase B component, a phase C component and a phase D component, wherein the phase A component comprises acrylamide dimethyl ammonium taurate/VP copolymer, glycerol, xanthan gum, sodium hyaluronate and 1, 3-propylene glycol; the phase B component comprises glycerol stearate, PEG-100 stearate, squalane and cyclopentadimethylsiloxane; the phase C component comprises 1, 2-hexanediol and p-hydroxyacetophenone; the phase D component comprises the composition.

10. A method for preparing a cosmetic product according to claim 9, comprising the steps of:

mixing the phase A components, and heating to dissolve to obtain a phase A mixture;

mixing the phase B components, and heating to dissolve to obtain a phase B mixture;

slowly adding the phase A mixture into the phase B mixture, homogenizing and cooling;

cooling to 60-65 deg.C, adding phase C component, and stirring;

and (3) cooling to below 40 ℃, adding the phase D component, and uniformly stirring to obtain the cosmetic.