CN114469801B

CN114469801B - Agilawood shampoo for regulating scalp microecological balance and preparation method thereof

Info

Publication number: CN114469801B
Application number: CN202210059888.0A
Authority: CN
Inventors: 白发平; 陈斌; 钱雪; 黄晓德; 王志康; 陈木红
Original assignee: Nanjing Shangyuantang Chenxiang Biotechnology Co ltd
Current assignee: Nanjing Shangyuantang Chenxiang Biotechnology Co ltd
Priority date: 2022-01-19
Filing date: 2022-01-19
Publication date: 2023-06-06
Anticipated expiration: 2042-01-19
Also published as: CN114469801A

Abstract

The invention discloses agilawood shampoo with a scalp microecological balance adjusting function and a preparation method thereof. The invention cleans and mixes agilawood, liquorice, ginseng, xanthium, achyranthes and drynaria; adding water 1-50 times of the mixed raw materials, pulverizing and grinding on a water mill, standing for layering, and separating oil layer to obtain extract; adding appropriate amount of ethanol into the above prepared extractive solution, standing, filtering, and recovering ethanol from filtrate to obtain refined solution; freezing and concentrating the refined liquid to obtain concentrated liquid, preparing the traditional Chinese medicine extract, obtaining plant extract, and adding proper shampoo raw materials to prepare the shampoo. The shampoo has the functions of removing dandruff, removing oil, relieving itching and the like by utilizing the functions of antioxidation, antibiosis, anti-inflammatory and the like of Chinese medicaments such as agilawood, liquorice and the like and good fragrance, and adding ginseng, cocklebur grass, achyranthes root, drynaria rhizome and the like into the shampoo formula, and meanwhile, experiments prove that the shampoo has a good effect of balancing scalp micro-ecology.

Description

Agilawood shampoo for regulating scalp microecological balance and preparation method thereof

Technical Field

The invention belongs to the field of shampoo, and particularly relates to agilawood shampoo for regulating scalp microecological balance and a preparation method thereof.

Background

The agalloch eaglewood is high-value wood containing resin, namely radix aquilariae sinensis Aquilaria sinensis (Lour.) belonging to the daphne family, has pungent and bitter taste and slight warmth, has the effects of promoting qi circulation and relieving pain, warming and stopping vomiting, and relieving asthma, is used for treating symptoms such as chest and abdomen distress and pain, gastric cold and vomiting and singultus, kidney deficiency and dyspnea, has the medical care effect, is a medicine capable of dispersing qi and tranquillizing, is qi sinking and coagulating, is not dispersed for a long time, is refreshing and can reduce blood fat and calm the liver, promote spleen and stomach, regulate chest and lung, has the functions of calming mind, ventilating pulse and the like, and is mainly distributed in the provinces of Guangdong, guangxi, hainan, yunnan, taiwan and the like in China. The Chinese herbal medicine is firstly carried in Liang Daitao Honggang Jing Yi Bie Lu (Ming Yi Bie Lu), listed as the top grade, and is a precious medicinal material and a traditional natural spice.

Researches show that the agilawood mainly contains chemical components such as sesquiterpenes, 2- (2-phenethyl) chromones, aromatic and the like, and pharmacological effects show that the agilawood has therapeutic effects on diseases related to digestive system, cardiovascular system, respiratory system and central nervous system, and has activities such as oxidation resistance, bacteriostasis, pain relieving, sedation, anti-inflammatory and anticancer and the like. The agilawood has the functions of sterilizing, diminishing inflammation and inhibiting tinea capitis, can effectively inhibit the ecdysis of hair epidermal cells, protects the color, has good stability, can exert efficacy for a long time, and has the effect of removing dandruff. Beta-caryophyllene in agilawood has inhibitory activity on the growth of 6 human pathogenic bacteria, 2 fungi (bacillus cereus, bacillus subtilis, staphylococcus aureus, escherichia coli, klebsiella pneumoniae, pseudomonas aeruginosa and rhizopus). The foundation is provided for the application of agilawood in shampoo.

The scalp is a unique ecological place compared with the skin at other parts of human body, and has a density of about 10 ³ ～10 ⁵ CFU/mm ² The microbial cells of (2) includeBacteria, fungi and viruses, which are mainly present on the scalp surface and in the skin appendages such as hair follicles, sebaceous glands, and the complex ecosystem composed of these microorganisms and hosts is called scalp microecology. The major bacterial flora on the scalp surface is propionibacterium acnes and staphylococcus epidermidis, and the major fungal microorganisms are malassezia restricta and malassezia globosa. The damage of the microbial balance state of the scalp can change the physiological environment of the scalp and damage the barrier function of the scalp, when the barrier function of the scalp is damaged, on one hand, external stimulus more easily permeates into the stratum corneum, and inflammatory reaction and abnormal desquamation of the scalp are caused; on the other hand, changes in scalp pH, secretion of antibacterial peptide, etc. caused by damage of the barrier may cause changes in scalp microorganism composition and microorganism activity, and finally appear as occurrence or deterioration of scalp sensitivity phenomenon. In recent years, in addition to scalp microorganisms, many scholars gradually transfer their eyes to the barrier function of the scalp stratum corneum, that is, the levels of scalp structural lipids, related proteins, natural moisturizing factors and the like are reduced due to external stimulus or self factors, so that the scalp hydration is further weakened, the TEWL value is increased, desquamation is abnormal and the like.

In the past, people's consciousness and behavior often only pay attention to hair care, so that scalp care is ignored; it is unknown that the scalp problems such as itching, dandruff, oil production, peculiar smell, erythema, alopecia, aging and the like can be caused by factors such as bad work and rest habits, external pressure, atmospheric pollution, surfactants, other chemical raw materials and the like. The health of the scalp directly affects the state of the hair, and the cuticle of the scalp is thinner than the face and the sebaceous glands are more abundant, so the scalp care requirement is higher. The scalp care product needs to have mild and clean, effective anti-drop, effective anti-dandruff, anti-aging and other functions on scalp, so as to protect scalp microecological environment, repair scalp barrier function, enhance scalp resistance and other aspects, maintain scalp normal metabolism function, and promote hair healthy growth.

CN110585283a discloses a traditional Chinese medicine formula for regulating scalp microecological balance and a preparation method thereof, wherein the shampoo is composed of Chinese angelica, biota orientalis, kuh-seng, magnolia officinalis and China rose, and the components are pure natural substances, do not have any chemical additives, are safe to use and have no toxic or side effects. However, the extraction process is complex, and large-scale production is difficult to realize. Moreover, in the effect evaluation, the evaluation conditions for the shampoo performance are too general, visual safety evaluation such as crowd or volunteer test is lacked, and the safety of the shampoo to the scalp of a human body is difficult to ensure.

Disclosure of Invention

The invention aims to overcome the defects of the prior art and provides agilawood shampoo for regulating scalp microecological balance and a preparation method thereof.

In order to achieve the purpose of the invention, the technical scheme adopted by the invention is as follows:

the preparation method of the agilawood shampoo for regulating scalp microecological balance is characterized by comprising the following steps of:

dispersing the phase A component in cold water completely, heating to 70-85 ℃, and stirring uniformly; sequentially adding the phase B components, and uniformly stirring; cooling to below 60 ℃, and adding phase C; adding the phase D component, and regulating the pH value; cooling to below 45 ℃, sequentially adding the E phase component and the F phase component, and uniformly stirring to obtain a product;

the phase A comprises water, polyquaternium-10 and hydroxypropyl guar gum trimethyl ammonium chloride; the phase B comprises disodium EDTA, cocamidopropyl betaine, sodium laureth sulfate, cocoyl monoethanolamine, cetostearyl alcohol, ethylene glycol distearate and allantoin; the C phase comprises an acrylic copolymer; the D phase comprises citric acid/sodium citrate buffer; the E phase comprises PEG-14M, methyl chloroisothiazolinone, essence, polydimethylsiloxane alcohol and polydimethylsiloxane; the phase F comprises traditional Chinese medicine extract;

the traditional Chinese medicine extract is prepared by the following method: cleaning lignum Aquilariae Resinatum, glycyrrhrizae radix, ginseng radix, herba Xanthii, achyranthis radix and rhizoma Drynariae, and mixing; adding water into the mixed raw materials, pulverizing and grinding on a water mill, standing for layering, and separating an oil layer to obtain an extracting solution; adding appropriate amount of ethanol into the above prepared extractive solution, standing, filtering, and recovering ethanol from filtrate to obtain refined solution; and (3) freezing and concentrating the refined liquid to obtain the product.

As a preferred scheme, the preparation method of the shampoo for regulating scalp microecology balance comprises the steps of completely dispersing the phase A component in cold water, heating to 80 ℃, and uniformly stirring; sequentially adding the phase B components, and uniformly stirring; cooling to below 60 ℃, and adding phase C; adding the phase D component, and adjusting the pH value to 7.0; cooling to 45 ℃, sequentially adding the components of the E phase and the F phase, and uniformly stirring to obtain the product.

In the preferred scheme, the shampoo preparation method for regulating scalp microecological balance comprises the steps of agilawood, liquorice, ginseng, cocklebur grass, achyranthes root and drynaria rhizome in a mass ratio of 2:4:3:3:2:4.

As a preferred scheme, the preparation method of the shampoo for regulating scalp microecological balance is characterized in that the mixed raw material medicines are added with 1-50 times of water and crushed and ground on a water mill until the raw material medicines are completely crushed.

Preferably, the method for preparing the shampoo for regulating the micro-ecological balance of the scalp is characterized in that ethanol is added into the prepared extracting solution, so that the ethanol content of the solvent is 65-75%, and the shampoo is kept stand for 24 hours and filtered. Adding rotary evaporation to recover ethanol at 55-60 deg.c until no smell of ethanol. The freezing temperature of the freeze concentration is-6 to-2 ℃, and the discharging temperature is-5 to-3 ℃.

Compared with the prior art, the application has the following beneficial effects:

the invention provides agilawood shampoo for regulating scalp microecological balance and a preparation method thereof, and the agilawood shampoo has the advantages that several traditional Chinese medicines with obvious efficacy and mild efficacy, such as agilawood, liquorice, ginseng, cocklebur grass, achyranthes root, drynaria rhizome and the like are screened, so that the effects of regulating scalp microecological balance, reconstructing scalp flora balance, enhancing scalp stratum corneum barrier function are achieved, and the effects of effectively relieving itching and removing dandruff, effectively inhibiting malassezia furfur, propionibacterium acnes and balancing grease and promoting scalp ecological maintenance and stabilization are achieved.

Detailed Description

For the purpose of making the objects, technical solutions and advantages of the embodiments of the present invention more apparent, the embodiments of the present invention will be described below.

Example 1

A preparation method of agilawood shampoo for regulating scalp microecological balance comprises the following steps:

(1) Extracting traditional Chinese medicine: cleaning lignum Aquilariae Resinatum, glycyrrhrizae radix, ginseng radix, herba Xanthii, achyranthis radix and rhizoma Drynariae at mass ratio of 2:4:3:3:2:4, and mixing; adding water 30 times of the mixed raw materials, pulverizing and grinding on a water mill until pulverizing, standing for layering, and separating oil layer to obtain extract; adding proper amount of ethanol into the prepared extracting solution to ensure that the ethanol content of the solvent is 65-75%, standing for 24 hours, and filtering; recovering ethanol by rotary evaporation at 55-60 ℃ until no ethanol smell exists, thus obtaining refined liquid; freezing and concentrating the refined solution at the freezing temperature of-6 to-2 ℃ and the discharging temperature of-5 to-3 ℃ to obtain concentrated solution, and preparing the traditional Chinese medicine extract.

(2) Dispersing the phase A component in cold water completely, heating to 80 ℃ and stirring uniformly according to the mass percentage of the following table 1; sequentially adding the phase B components, and uniformly stirring; cooling to below 60 ℃, and adding phase C; adding the phase D component, and adjusting the pH to 7.0; cooling to 45 ℃, sequentially adding the E phase component and the F phase component, and uniformly stirring to obtain the product.

Table 1 mass percent composition of each raw material

Comparative example 1

The comparative example provides a shampoo preparation method for adjusting scalp microecological balance, which is different in that the plant extract does not contain agilawood, and the other conditions are exactly the same as in example 1.

Comparative example 2

The comparative example provides a preparation method of agilawood shampoo for regulating scalp microecological balance, which is different in that the plant extract does not contain liquorice, and other conditions are exactly the same as in example 1.

Comparative example 3

The comparative example provides a preparation method of agilawood shampoo for regulating scalp microecological balance, which is different in that the plant extract does not contain ginseng, and other conditions are exactly the same as in example 1.

Comparative example 4

The comparative example provides a preparation method of agilawood shampoo for regulating scalp microecological balance, which is different in that the plant extract does not contain xanthium herb, and other conditions are exactly the same as in example 1.

Comparative example 5

The comparative example provides a preparation method of agilawood shampoo for regulating scalp microecological balance, which is different in that achyranthes root is not contained in plant extracts, and other conditions are exactly the same as in example 1.

Comparative example 6

The comparative example provides a preparation method of agilawood shampoo for regulating scalp microecological balance, which is different in that the plant extract does not contain drynaria rhizome, and other conditions are exactly the same as in example 1.

Comparative example 7

The comparative example provides a preparation method of agilawood shampoo for regulating scalp microecological balance, which is different in that no ethanol precipitation is added to the plant extract, and other conditions are exactly the same as in example 1.

Comparative example 8

The comparative example provides a preparation method of agilawood shampoo for regulating scalp microecological balance, which is different in that plant extracts are not subjected to freeze concentration, and other conditions are exactly the same as in example 1.

Example 2 in vitro bacteriostasis test

1. Testing strains: malassezia furfur, propionibacterium acnes

2. Pouring solid culture medium into culture dish by filter paper sheet method, and pouring bacteria with concentration of 10 into the culture medium after solidification ⁵ ～l0 ⁶ CFU/mL malassezia furfur and Propionibacterium acnes hydroponic cultureAnd (5) nourishing. 3 filter paper sheets were placed equidistantly on the medium, and 3 replicates were run for each sample using normal saline as a normal growth control. After culturing in an incubator at 30℃for 2 days, the diameter of the inhibition zone of each of the agilawood shampoos of example 1 and comparative examples 1 to 8 was measured with a ruler, and the average value was taken.

3. The experimental results are shown in table 2 below:

TABLE 2 antibacterial test results

As can be seen from the table, the agilawood shampoo for regulating scalp microecological balance prepared by the invention effectively inhibits the growth of malassezia furfur and propionibacterium acnes, has remarkable effect, can ensure that the scalp is clean, and does not cause the problems of dandruff and itching. And the overall effect is more than that of comparative examples 1 to 7. Illustrates the scientific synergistic antibacterial effect of the traditional Chinese medicine raw materials.

Example 3 measurement of scalp surface fat

1. The purpose of the experiment is as follows: whether the composition can reach sebum balance by adjusting scalp microecology balance is judged by measuring scalp surface fat, and scalp oil discharge phenomenon is reduced.

2. The experimental method comprises the following steps: the shampoo of example 1 and comparative examples 1-8 was used for two consecutive weeks, once every three days, to recruit 90 volunteers with significant head oil. The temperature of the laboratory was controlled at 20℃and the air humidity was controlled at 40%, the subject did not engage in vigorous exercise for 3 hours prior to sebum determination, no obvious sweating, and sitting still in the laboratory for 20min. Scalp surface lipid amounts were measured at 72h after shampooing at week 2, respectively. The test part takes 6 points, is about 3cm away from the two sides of the midline of the scalp, and is longitudinally and respectively positioned at the position 3cm away from the anterior hairline, the parietal tuberosity and the occipital tuberosity. The test method comprises removing hair to expose scalp, measuring 0.1mm thick and 64mm thick with SEBUMETER SM810 ² The tape was read after 30s pressing at the measurement site. The test results are shown in the following table:

TABLE 3 results of improved scalp surface lipid level test

The experimental data show that the agilawood shampoo for regulating scalp microecological balance can inhibit scalp grease and reduce scalp oil discharge.

Example 4 human body patch test

1. Test article: shampoo of example 1 of the present invention, shampoo of comparative examples 1 to 8;

2. the subject: 90 persons, wherein the ages of men and women are 20-50 years, and the male and female persons meet the volunteer selection standard of the subjects;

3. the test method comprises the following steps: placing a test object into a proper patch tester by adopting a closed test method, applying the test object to the curved side of the arm of a subject by using a hypoallergenic adhesive tape, and uniformly applying the test object to the skin by using palm light pressure for 24 hours; the blank group is a subject without using the product;

4. observation results: the test pieces were removed after 24 hours, and skin reactions were observed after 0.5 hours, 24 hours, and 48 hours, respectively, and the reaction results were recorded.

The adverse reaction grade of the skin is as follows:

5. the results were counted as follows:

the experimental data show that the agilawood shampoo for regulating scalp microecological balance has no toxic effect on skin, and the prepared shampoo is good in safety and easy to use.

Claims

1. The preparation method of the agilawood shampoo for regulating scalp microecological balance is characterized by comprising the following steps of:

(1) Extracting traditional Chinese medicine: cleaning lignum Aquilariae Resinatum, glycyrrhrizae radix, ginseng radix, herba Xanthii, achyranthis radix and rhizoma Drynariae at mass ratio of 2:4:3:3:2:4, and mixing; adding water 30 times of the mixed raw materials, pulverizing and grinding on a water mill until pulverizing, standing for layering, and separating oil layer to obtain extract; adding a proper amount of ethanol into the prepared extracting solution, enabling the alcohol content of a solvent to be 65-75%, standing for 24 hours, and filtering; recovering ethanol by rotary evaporation at 55-60 ℃ until no ethanol smell exists, and obtaining refined liquid; freezing and concentrating the refined solution at the freezing temperature of-6 to-2 ℃ and the discharging temperature of-5 to-3 ℃ to obtain concentrated solution, and preparing a traditional Chinese medicine extract;

(2) According to the mass percentage of the table, firstly dispersing the phase A component in cold water completely, heating to 80 ℃ and stirring uniformly; sequentially adding the phase B components, and uniformly stirring; cooling to below 60 ℃, and adding phase C; adding the phase D component, and adjusting the pH to 7.0; cooling to 45 ℃, sequentially adding the components of the E phase and the F phase, and uniformly stirring to obtain the composite material;

。

2. the application of the agilawood shampoo prepared by the preparation method according to claim 1 in preparing an external preparation for regulating sebum balance.