CN114469766A - A cosmetic composition containing glucose electrolyte for moisturizing and improving skin barrier - Google Patents

Abstract

The invention provides a cosmetic composition containing a glucose electrolyte and capable of moisturizing and improving skin barriers, relates to the technical field of cosmetic compositions containing electrolytes, and provides a cosmetic composition containing sodium gluconate, potassium gluconate and calcium gluconate and capable of moisturizing and improving skin barriers. The electrolyte is different from the traditional electrolyte consisting of sodium chloride, potassium chloride and calcium chloride in that the solution of sodium gluconate, potassium gluconate and calcium gluconate has small conductivity and small influence on the stability of a cosmetic formula system, can effectively promote the synthesis of ceramide, improves the weakening and aging of a skin barrier caused by external stimulation, has very excellent effects of immediately increasing the skin moisturizing capability and the sustaining force, and can be used for improving the skin barrier and moisturizing the skin.

Description

A cosmetic composition for moisturizing and improving skin barrier containing glucose electrolyte

technical field

The present invention relates to the technical field of cosmetic compositions containing electrolytes, and in particular, provides cosmetic compositions for moisturizing and improving skin barrier including sodium gluconate, potassium gluconate and calcium gluconate.

Background technique

The skin is an organ that is in constant contact with the external environment of the body, and it first performs the function of a physical barrier. The skin is roughly divided into three layers: epidermis, dermis, and subcutaneous fat. The stratum corneum of the epidermis can inhibit the loss of water and electrolytes, thereby preventing skin dryness and providing an environment for normal skin biochemical metabolism. In addition, it also protects the body from humidity and temperature changes, ultraviolet rays, and other physical or chemical environmental stimuli.

The keratinocyte layer that constitutes the normal skin barrier will appear smooth, moist and healthy if the moisture content is maintained at about 10-30%. But if the moisture content of the stratum corneum falls below 10%, the skin becomes dry, cracked, rough, and looks old. If the skin does not maintain a certain amount of moisture and electrolytes, the acidity and osmolarity of the skin will be reduced, the skin will be dry, and it will become sensitive to small stimuli, resulting in symptoms such as itching, tingling, and inflammation. To improve such a damaged skin barrier, cosmetics can be used to supply the skin with lost oil and moisture. So far, research on cosmetic products that provide oil and moisture to the skin has been active, but research on cosmetic products including electrolytes and their components that provide immediate and rapid recovery when applied to the skin is still necessary.

If the balance of the skin's electrolytes is disrupted, the skin will continue to dry out even with more makeup on. When the osmotic pressure of cosmetics is higher than that of the skin, the water in the skin cells flows out due to the osmotic pressure, and the skin becomes dry. On the contrary, when the cosmetic osmotic pressure is lower than the skin osmotic pressure, the water will be excessively transferred into the cells, and the cells will swell and destroy due to excessive absorption of water. Therefore, similar to the electrolyte and osmotic pressure in the human body, when applied to the skin, it can help the skin to restore health immediately and quickly, maintain the lasting stability of the skin, and improve the skin barrier and moisturizing cosmetic composition research and development is necessary.

SUMMARY OF THE INVENTION

In order to solve the problem of dry skin surface, the present invention provides a cosmetic composition comprising sodium gluconate, potassium gluconate and calcium gluconate for moisturizing and improving skin barrier, so as to reduce the influence on the stability of the cosmetic formulation system.

The technical scheme of the present invention is: a cosmetic composition containing glucose electrolyte for moisturizing and improving skin barrier, the weight of sodium gluconate is 0.5-1.5% (w/v) of the total weight of the composition, and the weight of potassium gluconate is 0.01-0.1% (w/v) of the total weight of the composition, and the weight of calcium gluconate is 0.005-0.1% (w/v) of the total weight of the composition. The above-mentioned sodium gluconate, potassium gluconate and calcium gluconate are mixed in a weight ratio of 85-97:1-5:0.5-5.

As an optional embodiment of the present invention, the above-mentioned sodium gluconate, potassium gluconate and calcium gluconate are mixed in a weight ratio of 87-97:1.0-5:2-5.

As an optional embodiment of the present invention, the above-mentioned sodium gluconate, potassium gluconate and calcium gluconate are mixed in a weight ratio of 90-97:1.0-4:2-5.

As an optional embodiment of the present invention, the above-mentioned sodium gluconate, potassium gluconate and calcium gluconate are mixed in a weight ratio of 90-95:2.0-5:2-4.

As an optional embodiment of the present invention, the above-mentioned sodium gluconate, potassium gluconate and calcium gluconate are mixed according to the weight ratio of 87-95:2.0-4:2-4.

As an optional embodiment of the present invention, the above-mentioned sodium gluconate, potassium gluconate and calcium gluconate are mixed according to the weight ratio of 90-95:2.0-5:2-3.

As an optional embodiment of the present invention, the above-mentioned sodium gluconate, potassium gluconate and calcium gluconate are mixed according to the weight ratio of 92-95:2.0-4:2-3.

Description of drawings

Fig. 1 is the measurement result of CerS3 gene expression level;

Fig. 2 is the viscosity test comparison result of Example 2, the comparative example, and the blank control example.

Detailed ways

The present invention will be further described in detail below with reference to the accompanying drawings and embodiments. It should be understood that the specific embodiments described herein are only used to explain the present invention, but not to limit the present invention.

Example 1

A cosmetic composition for moisturizing and improving skin barrier containing glucose electrolyte, sodium gluconate, potassium gluconate and calcium gluconate are mixed in a weight ratio of 95:3:2. Dissolve 9.5 g of sodium gluconate, 3 g of potassium gluconate, and 2 g of calcium gluconate in 1 L of water, respectively.

In Example 1, sodium gluconate, potassium gluconate and calcium gluconate are included, which can effectively promote ceramide synthesis, improve skin barrier weakening and aging caused by external stimuli, and instantly increase skin moisturizing power and sustainability. , can be used to improve skin barrier and skin moisturizing.

Example 2

A cosmetic composition for moisturizing and improving skin barrier containing glucose electrolyte, sodium gluconate, potassium gluconate and calcium gluconate are mixed in a weight ratio of 95:3:2. In the comparative example, sodium chloride, potassium chloride, and calcium chloride were mixed in a weight ratio of 95:3:2. Dissolve 9.5 g of sodium gluconate, 3 g of potassium gluconate, and 2 g of calcium gluconate in 1 L of water, respectively. Dissolve 9.5g of sodium chloride, 3g of potassium chloride and 2g of calcium chloride in 1L of water respectively.

In embodiment 2, a certain proportion of sodium gluconate, potassium gluconate, calcium gluconate and the same proportion of sodium chloride, potassium chloride, calcium chloride are made into a solution, and added to the cosmetic products that are not resistant to ions, The viscosity of the product of the comparative example decreased significantly. Therefore, the composition comprising sodium gluconate, potassium gluconate, and calcium gluconate reduces the impact on the stability of the cosmetic formulation system compared to the composition of sodium chloride, potassium chloride, and calcium chloride.

Moisturizing and Barrier Improvement Efficacy Cell Experiment

When skin ages, ceramides, an important component responsible for 40% of the lipids in the skin's barrier, are significantly reduced, along with changes in hydration and the skin's barrier. In this experiment, in order to measure the effects of skin moisturizing and skin barrier improvement, human keratinocytes were used to measure the expression level of CerS3, an enzyme that synthesizes ceramides, at the mRNA level.

(1) Preparation of samples

The sample of 0.2 μm produced in the above Example 1 was filtered with a syringe filter. The cell culture medium was added to the cell culture medium to bring the above final concentration to 10% (v/v). Pure distilled water was added as a control group.

(2) Human keratinocyte culture

Keratinocytes, HaCaT cells, are grown in Dulbecco's modified Eagle's medium (DMEM), the most widely used medium for skin cell culture. Cells were cultured in 100 mm dishes, mixed with 10% fetal bovine serum (FBS) and 1% penicillin. The cultures containing the above samples were incubated for 24 hours in an incubator at 37°C, 5% CO2 concentration and 95% humidity.

(3) Experiment of skin moisturizing effect

RNA Mini Kit(Thermo Fisher Scientific)提取总RNA。基因的表达变化是用在聚合酶连锁反应(PCR)中扩大的DNA产物上结合荧光物质，持续检测荧光物质的qRT-PCR(quantitative real time PCR)进行测定的。为控制神经酰胺合成酶CerS3基因的表达量，通过SYBR green(Thermo Fisher Scientific)测定PCR产物发出的荧光值。在PCR tube中将0.1μM引物、50mM KCl、20mM Tris/HCl pH 8.4、0.8mM dNTP、0.5U Extaq DNA聚合酶、3mM MgCl2,1×SYBR green进行混合制成反应液。使用PCR仪器，在94℃下进行3分钟预变性(primary denaturation)后，在94℃下30秒，58℃下30秒，72℃下30秒共进行40次循环的变性(denaturation)、退火(annealing),聚合(polymerization)。每轮结束后测定荧光值。将CerS3因子的荧光值用GAPDH值进行标准化，将对照组进行100％换算后比较差异。实验中使用的引物序列见表1。The cells cultured in the above-mentioned (1) culture solution were recovered, washed with cooled phosphate buffered saline (PBS), and used

Total RNA was extracted with RNA Mini Kit (Thermo Fisher Scientific). Changes in gene expression are measured by qRT-PCR (quantitative real time PCR) that continuously detects fluorescent substances by binding fluorescent substances to DNA products amplified in a polymerase chain reaction (PCR). In order to control the expression level of the ceramide synthase CerS3 gene, the fluorescence value emitted by the PCR product was measured by SYBR green (Thermo Fisher Scientific). In a PCR tube, mix 0.1 μM primer, 50 mM KCl, 20 mM Tris/HCl pH 8.4, 0.8 mM dNTP, 0.5 U Extaq DNA polymerase, 3 mM MgCl 2 , and 1×SYBR green to prepare a reaction solution. Using a PCR instrument, after 3 minutes of primary denaturation at 94°C, 40 cycles of denaturation and annealing were performed at 94°C for 30 seconds, 58°C for 30 seconds, and 72°C for 30 seconds. annealing), polymerization (polymerization). Fluorescence values were measured after each round. The fluorescence value of the CerS3 factor was normalized with the GAPDH value, and the difference was compared after converting the control group to 100%. The primer sequences used in the experiments are shown in Table 1.

【Table 1】

Fig. 1 shows the measurement results of the expression level of CerS3 gene.

As shown in FIG. 1 , the expression level of the CerS3 gene in Example 1 was remarkably high. The cosmetic composition containing sodium gluconate, potassium gluconate and calcium gluconate can effectively promote the synthesis of ceramide and improve skin moisturizing and barrier function.

Reduced effects on the stability of cosmetic formulation systems (viscosity)

In Example 2, 9.5 g of sodium gluconate, 3 g of potassium gluconate, and 2 g of calcium gluconate were dissolved in 1 L of water, respectively, and named solution A. Dissolve 9.5g of sodium chloride, 3g of potassium chloride, and 2g of calcium chloride in 1L of water respectively, named solution B. The blank control group C was pure water. Three solutions of A, B, and C were used to dissolve 0.5% carbomer 941 (intolerant of ions) powder, neutralize it with 0.75% triethanolamine, and test its viscosity. The results are shown in Table 2.

【Table 2】

group Viscosity (cps) A 32000 B 18000 C 35000

As shown in Figure 2, in Example 2, the cosmetic composition comprising sodium gluconate, potassium gluconate and calcium gluconate has much less influence on the viscosity, which can effectively reduce the stability of the cosmetic formulation system. influences.

The above description describes the preferred embodiments of the present invention. As mentioned above, it should be understood that the present invention is not limited to the form disclosed herein, and should not be regarded as the exclusion of other embodiments, but can be used in various other combinations, modifications and environment, and can be modified within the scope of the inventive concept described herein, from the above teachings or from skill or knowledge in the relevant field. However, modifications and changes made by those skilled in the art do not depart from the spirit and scope of the present invention, and should all fall within the protection scope of the appended claims of the present invention.

Claims (8)

1. a cosmetic composition containing glucose electrolyte for moisturizing and improving skin barrier, is characterized in that: the weight of sodium gluconate is 0.5-1.5% (w/v) of the composition gross weight, and the weight of potassium gluconate is composed of The weight of calcium gluconate is 0.01-0.1% (w/v) of the total weight of the composition, and the weight of calcium gluconate is 0.005-0.1% (w/v) of the total weight of the composition. 2. a kind of cosmetic composition containing the moisturizing of glucose electrolyte and improving skin barrier according to claim 1, is characterized in that: above-mentioned sodium gluconate, potassium gluconate, calcium gluconate are according to 85-97:1- 5:0.5-5 weight ratio for mixing. 3. a kind of cosmetic composition containing glucose electrolyte for moisturizing and improving skin barrier according to claim 2, is characterized in that: above-mentioned sodium gluconate, potassium gluconate, calcium gluconate are according to 87-97:1.0- 5:2-5 weight ratio for mixing. 4. a kind of cosmetic composition containing glucose electrolyte for moisturizing and improving skin barrier according to claim 1, is characterized in that: above-mentioned sodium gluconate, potassium gluconate, calcium gluconate are according to 90-97:1.0- 4:2-5 weight ratio for mixing. 5. a kind of cosmetic composition containing glucose electrolyte for moisturizing and improving skin barrier according to claim 1, is characterized in that: above-mentioned sodium gluconate, potassium gluconate, calcium gluconate are according to 90-95:2.0- Mixed in a weight ratio of 5:2-4. 6. a kind of cosmetic composition containing glucose electrolyte for moisturizing and improving skin barrier according to claim 2, is characterized in that: above-mentioned sodium gluconate, potassium gluconate, calcium gluconate are according to 87-95:2.0- 4:2-4 weight ratio for mixing. 7. a kind of cosmetic composition containing glucose electrolyte for moisturizing and improving skin barrier according to claim 2, is characterized in that: above-mentioned sodium gluconate, potassium gluconate, calcium gluconate are according to 90-95:2.0- 5:2-3 weight ratio for mixing. 8. a kind of cosmetic composition containing glucose electrolyte for moisturizing and improving skin barrier according to claim 2, is characterized in that: above-mentioned sodium gluconate, potassium gluconate, calcium gluconate are according to 92-95:2.0- 4:2-3 weight ratio for mixing.

Images