CN114457136A - Fermentation liquid based on rose fermentation recombinant collagen and application thereof - Google Patents
Fermentation liquid based on rose fermentation recombinant collagen and application thereof Download PDFInfo
- Publication number
- CN114457136A CN114457136A CN202210101822.3A CN202210101822A CN114457136A CN 114457136 A CN114457136 A CN 114457136A CN 202210101822 A CN202210101822 A CN 202210101822A CN 114457136 A CN114457136 A CN 114457136A
- Authority
- CN
- China
- Prior art keywords
- fermentation
- rose
- collagen
- recombinant collagen
- application
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Images
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P21/00—Preparation of peptides or proteins
- C12P21/02—Preparation of peptides or proteins having a known sequence of two or more amino acids, e.g. glutathione
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/96—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution
- A61K8/97—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution from algae, fungi, lichens or plants; from derivatives thereof
- A61K8/9783—Angiosperms [Magnoliophyta]
- A61K8/9789—Magnoliopsida [dicotyledons]
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q19/00—Preparations for care of the skin
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q19/00—Preparations for care of the skin
- A61Q19/08—Anti-ageing preparations
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
- C07K14/78—Connective tissue peptides, e.g. collagen, elastin, laminin, fibronectin, vitronectin or cold insoluble globulin [CIG]
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
- C12N5/06—Animal cells or tissues; Human cells or tissues
- C12N5/0602—Vertebrate cells
- C12N5/0625—Epidermal cells, skin cells; Cells of the oral mucosa
- C12N5/0629—Keratinocytes; Whole skin
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
- C12N5/06—Animal cells or tissues; Human cells or tissues
- C12N5/0602—Vertebrate cells
- C12N5/0652—Cells of skeletal and connective tissues; Mesenchyme
- C12N5/0656—Adult fibroblasts
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2800/00—Properties of cosmetic compositions or active ingredients thereof or formulation aids used therein and process related aspects
- A61K2800/80—Process related aspects concerning the preparation of the cosmetic composition or the storage or application thereof
- A61K2800/85—Products or compounds obtained by fermentation, e.g. yoghurt, beer, wine
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2500/00—Specific components of cell culture medium
- C12N2500/70—Undefined extracts
- C12N2500/76—Undefined extracts from plants
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2533/00—Supports or coatings for cell culture, characterised by material
- C12N2533/50—Proteins
- C12N2533/54—Collagen; Gelatin
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- General Health & Medical Sciences (AREA)
- Zoology (AREA)
- Genetics & Genomics (AREA)
- Biomedical Technology (AREA)
- Wood Science & Technology (AREA)
- Biotechnology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Microbiology (AREA)
- Biochemistry (AREA)
- Dermatology (AREA)
- General Engineering & Computer Science (AREA)
- Animal Behavior & Ethology (AREA)
- Veterinary Medicine (AREA)
- Public Health (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Molecular Biology (AREA)
- Cell Biology (AREA)
- Birds (AREA)
- Epidemiology (AREA)
- Mycology (AREA)
- Toxicology (AREA)
- Gastroenterology & Hepatology (AREA)
- Biophysics (AREA)
- Medicinal Chemistry (AREA)
- Botany (AREA)
- Rheumatology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- General Chemical & Material Sciences (AREA)
- Gerontology & Geriatric Medicine (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
The invention discloses a rose fermentation recombinant collagen-based fermentation liquid, which comprises the following components in percentage by mass: 10 to 30 percent of rose pollen and 70 to 90 percent of BSM culture medium. The invention also discloses application of the fermentation liquid, including application in fermentation production of collagen, application in fermentation production of fermentation products for promoting cell proliferation and growth, application in fermentation production of fermentation products for repairing skin injury and resisting aging and the like.
Description
Technical Field
The invention belongs to the technical field of biology, and particularly relates to fermentation liquor based on rose fermentation recombinant collagen and application thereof.
Background
Fermentation is the process by which microorganisms live in aerobic or anaerobic conditions to produce the microbial cells themselves, or direct or secondary metabolites. Fermentation was the earliest of the origin of food spoilage and later utilized by humans to ferment a variety of flavors of foods. Modern fermentation technology has evolved into an engineering discipline and independent industry. The technology of directional fermentation with natural plants or food is continuously being developed. For example, the ferment food fermentation technology is a technology for fermenting fruits and vegetables compositely by using lactic acid bacteria, yeasts and the like to form liquid or solid food rich in lipase, amylase, protease, metabolites such as lactic acid and acetic acid. Plants are usually able to provide specific nutrients for bacterial growth during fermentation, e.g. inorganic salts, vitamins, and may also induce specific metabolites in microorganisms.
Roses, as a natural plant raw material, have been used by people for beauty and skin care since ancient times, contain volatile oil, namely rose essential oil, and also contain a plurality of non-volatile chemical components mainly comprising flavonoid components, and the secondary metabolites have various physiological activities and are often applied to cosmetics, food industries and medicines. The rose petals are rich in anthocyanin, flavonoid substances, volatile oil and fat, polyphenol substances, various vitamins, amino acids, trace elements, alkaloid and the like, and have high edible value and medicinal value. Roses have a long history in China, are medicinal and edible flowers in China, and are widely used for foods, wine soaking, tea making, beauty treatment, cosmetics, traditional Chinese medicines and the like. Products such as rose ferment fermentation, rose honey wine brewing and the like are widely accepted by the market.
Pichia pastoris is used as an engineering strain, and can directionally generate a specific metabolite after being genetically modified, and the recombinant human collagen is widely obtained in Pichia pastoris engineering bacteria. However, the fermentation of the existing engineering bacteria usually adopts expensive nutritional ingredients such as yeast powder and peptone, and the fermentation liquid after production has heavy smell and is difficult to use directly, and is usually removed after fermentation, thereby causing waste and environmental pollution.
The organic matter component in the rose is used as the nutrient needed by the growth of the recombinant humanized collagen engineering strain to realize biological fermentation, and the achievement provides important application value for technical innovation in the fields of beauty treatment, food or medicine. In the present invention, we named the fermentation broth product "rose collagen fermentation broth".
Disclosure of Invention
Aiming at the bottleneck problems, the invention uses natural plant raw materials as nutrient components of the culture medium to provide nutrient substances for the strains in the fermentation process, and can also produce certain special metabolites, and the fermented culture solution can be used as a final product, thereby realizing the maximization of economic benefits and being green and environment-friendly. The method specifically comprises the following steps: the rose extract is added into a culture medium system for fermentation of pichia pastoris, roses are innovatively used as one of nutrient components for fermentation of recombinant collagen engineering bacteria, and the components in the extract delay the aging of the engineering bacteria, so that the yeast engineering bacteria are promoted to grow, and a higher collagen expression level is obtained. The fermentation product shows excellent effects of resisting oxidation and promoting cell proliferation and growth, has good regulating effect on various skin anti-aging genes, and has positive promoting effect on the whole skin anti-aging. Therefore, the first object of the present invention is to provide a fermentation liquid based on rose fermented recombinant collagen. The second purpose of the invention is to provide the application of the fermentation liquor.
In order to achieve the purpose, the invention adopts the following technical scheme:
as a first aspect of the invention, a fermentation liquid based on rose fermented recombinant collagen comprises the following components in percentage by mass: 10 to 30 percent of rose pollen and 70 to 90 percent of BSM culture medium.
According to the invention, the fermentation liquid based on the rose fermented recombinant collagen consists of the following components in percentage by mass: 20% of rose pollen and 80% of BSM culture medium.
As a second aspect of the invention, the fermentation liquid based on rose fermented recombinant collagen is applied to the fermentation production of collagen.
As a third aspect of the invention, the rose fermentation recombinant collagen-based fermentation broth is used for producing a fermentation product with the function of promoting cell proliferation.
As a fourth aspect of the invention, the fermentation broth based on the rose fermented recombinant collagen is applied to the fermentation production of a fermentation product with the functions of repairing skin damage and resisting aging.
As a fifth aspect of the invention, the rose fermentation recombinant collagen-based fermentation broth is applied to fermentation production of a fermentation product with moisturizing gene expression promotion function.
According to the present invention, the moisturizing genes include the genes AQP-3, CD44, and HAS 1.
The invention has the beneficial effects that:
1. the fermentation liquor does not need to be further purified, and the fermentation product can be directly externally applied to the surface of the skin, so that the method is economical and environment-friendly, and has remarkable effects on repairing skin injury and resisting aging;
2. when the fermentation broth is used for fermentation, the fermentation product has the effect of promoting cell proliferation and growth;
3. the fermentation liquor can be used for improving the expression quantity of collagen.
Drawings
FIG. 1 shows the expression level of collagen in rose fermentation broth containing different concentrations of rose pollen for 30 hours.
FIG. 2 shows the expression level of collagen in a culture medium containing 20% rose pollen.
FIG. 3 shows the amount of collagen expression induced in the fermentor of example 3.
FIG. 4 shows the yeast density in the fermenter according to example 3.
FIG. 5 is a graph showing the effect of rose collagen on cell proliferation. Wherein,
*p<0.05；**P<0.01；***P<0.001；****P<0.0001；
note: indicates that the sample has significant difference in comparison with the blank treatment group under the effect of the concentration.
FIGS. 6-8 show the effect of rose collagen on the expression of AQP-3 gene, CD44 gene and HAS-1 gene, respectively. Wherein,
*p<0.05；**P<0.01；***P<0.001；
note: indicates that the sample has significant difference in comparison with the blank treatment group under the effect of the concentration.
Detailed Description
The present invention is further illustrated by the following examples. The following examples are intended to illustrate the invention only and are not intended to limit the scope of the invention. The experimental procedures, in which specific conditions are not specified, in the following examples are generally conducted under conventional conditions, or under conditions provided by the manufacturers.
The recombinant human type III collagen Pichia engineering strain (GS115RHC04), available from Meinjin Biotech, Inc., of the following examples.
Example 1 preparation of Rose fermentation broth
The flos Rosae Rugosae is selected from Shandong Pingyin rose planting production area, and dried flos Rosae Rugosae is crushed into powder by crusher. A Rose-Based fermentation broth (RMSM) was prepared as follows. The formula of the rose fermentation culture solution is shown in table 1 in percentage by mass. When in use, the nutrient components in the formula are mixed and sterilized, but the metal inorganic salt is sterilized separately and then is mixed aseptically at normal temperature.
TABLE 1 Rose fermentation culture solution formula
Example 2 Shake flask fermentation of Rose collagen
Selecting recombinant human III-type collagen Pichia pastoris engineering strain, inoculating into 25ml BMGY culture medium, shaking 250ml, shaking at 30 deg.C and 250rpm to OD600Centrifugation at 3000g for 5min at RT for 4, cell collection, supernatant removal, resuspension of cells to OD using RMSM medium of different concentrations in Table 1600Induction expression was performed at 1.0 to obtain a culture. Adding the above into a 1L shake flaskThe culture is covered with two layers of sterilized gauze and put into a shaking table for continuous growth. Every 24 hours, methanol was added to a final concentration of 0.5% to continue induction. At various time points, 1ml of medium was taken to a 1.5ml centrifuge tube. These samples were used to analyze expression levels and to determine the optimal time to collect cells after induction. Centrifuging at room temperature for 2-3min at maximum rotation speed of horizontal centrifuge. For secretory expression, the supernatant was transferred to a separate tube and the supernatant and cell pellet were stored at-80 ℃ until detection was initiated. The supernatant protein expression was analyzed by Coomassie blue staining and SDS-PAGE. Through analysis, the recombinant human collagen can be normally expressed in the rose medium, the expression level is increased along with the increase of the proportion of rose pollen, but the expression level begins to decrease after the expression level exceeds 30 percent, and the figure is shown in figure 1. May be caused by the unbalanced nutrition of the microorganisms due to the unbalanced proportion of the other inorganic salt components in the BSM. Therefore, we selected a culture medium with 20% rose pollen content and the expression level increased with time, fermented for 30 hours, and the yield reached about 2 g/L as shown by SDS-PAGE electrophoresis staining, as shown in FIG. 2.
The subsequent experiment uses 20% rose flower pollen content rose flower fermentation culture solution.
EXAMPLE 3 Large Scale fermenter fermentation of collagen
Inoculating the frozen recombinant human III type collagen Pichia pastoris engineering strain into a sterilized and cooled YPD culture medium, and putting the YPD culture medium into a shaking table for activation culture for 28-30 h to ensure that the OD of the strain is600The value is between 3 and 5. Inoculating the activated strain into a 10L glass fermentation tank for amplification culture for 6-8 h. Transferring the amplified seed solution to a stainless steel fermentation tank containing 150L of RMSM culture medium according to the volume ratio of 1:10 (seed solution: fermentation liquor) for culturing for 16-18 h, and transferring into a BSM inorganic salt culture medium in a control experiment. Supplementing a culture medium in a fermentation tank in a feeding mode, and continuously culturing for 3-4 h to enable the wet weight of the bacteria to reach 150-180 g/L. Gradually adding methanol, maintaining the concentration of the methanol at 0.5%, and performing induction culture for 21-24 h. And centrifuging the induced fermentation liquor at a high speed, separating thalli and fermentation supernatant, and collecting the fermentation supernatant. SDS-PAGE shows that the yield of the collagen in the fermentation tank is obviously improved, which is mainly caused by the increase of the density of thalliAnd (4) adding. The expression level in RMSM was 4 g/L and about 3 g/L in BSM medium. The rose medium obviously improves the expression amount of the collagen. The results are shown in FIG. 3. The OD600 of the cell density was measured by a spectrophotometer, and the results are shown in FIG. 4. The results show that the growth rate of RMSM is lower than BSM in the growth process of the thallus, but the thallus density of the two culture media is equivalent in the final stage of fermentation, so that RMSM inhibits the rapid growth of the thallus and slows down the senescence of the thallus.
Example 4 promotion of cell proliferation
The rose collagen fermentation liquid obtained in example 3 was lyophilized into powder in a lyophilizer, and the powder was quantified and then prepared into solutions of different concentrations. 3T3 cells with good growth state (purchased from China university of science and technology laboratory) are taken and laid on a 96-well plate for culture for 17h, a serum-free basic culture medium is replaced for culture for 7h, the old culture medium is discarded, rose collagen solutions with different concentrations are added, and after 48h, a CCK8 method is adopted to detect 0D450nm, so that the influence of the detected sample on the proliferation of the 3T3 cells is obtained. See fig. 5.
The result shows that the rose collagen fermentation solution can obviously promote cell proliferation, reaches the maximum value at 0.08mg/mL, and has the effect on the cell proliferation equivalent to that of 10% biological FBS. It is demonstrated that the rose collagen fermentation product may produce various substances for promoting cell growth in addition to collagen.
Example 5 Effect on expression of genes involved in cellular moisturization
The rose collagen fermentation broth obtained in example 3 was lyophilized into powder in a lyophilizer, and the powder was quantified and then prepared into solutions of different concentrations using the complete medium. And (3) paving the hacat cells in a 6-hole plate, culturing for 24h, replacing the cells with samples to be detected with different concentrations, extracting total RNA by TRIzon after 24h, and detecting the RNA purity and concentration by using NanoDrop. Subsequently, cDNA was synthesized and amplified by PCR, and the band of the desired gene was confirmed by agarose gel electrophoresis analysis. And finally, the cDNA is used for Real-Time PCR quantitative determination to obtain the influence of the rose collagen on HACAT cell moisturizing related gene expression.
In this example, three genes, aquaporin-3 (AQP-3), cell surface glycoprotein (CD44) and hyaluronic acid synthase 1(HAS 1), were measured, and the expression of the three genes was positively correlated with the skin moisturizing function, and the expression level thereof reflected the effect on the skin moisturizing function to a certain extent. When the sample concentration is 0.05mg/ml, the agent expression amounts of the HAcat cell moisturizing related genes AQP-3, CD44 and HAS1 are 94%, 104% and 221% of the blank group respectively; when the concentration is 0.5mg/ml, the agent-expressed amounts of the HAcat cell moisturizing related genes AQP-3, CD44 and HAS1 are 120%, 125% and 320% of the blank group respectively, and the result HAS promotion effect. The results are shown in fig. 6, 7 and 8.
The foregoing is merely an example of the embodiment of the present invention, and it should be noted that, for those skilled in the art, several modifications and variations can be made without departing from the technical principle of the present invention, and these modifications and variations should also be regarded as the protection scope of the present invention.
Claims (7)
1. A fermentation liquid based on rose fermentation recombinant collagen is characterized by comprising the following components in percentage by mass: 10 to 30 percent of rose pollen and 70 to 90 percent of BSM culture medium.
2. The fermentation broth according to claim 1, which consists of the following components in percentage by mass: 20% of rose pollen and 80% of BSM culture medium.
3. Use of a rose fermentation recombinant collagen-based fermentation broth according to claim 1 or 2 for the fermentative production of collagen.
4. Use of a rose fermentation recombinant collagen-based fermentation broth according to claim 1 or 2 for the fermentative production of a fermentation product having enhanced cell proliferation.
5. Use of a fermentation broth based on rose fermented recombinant collagen according to claim 1 or 2 in the fermentative production of a fermentation product with skin damage repair and anti-aging properties.
6. Use of a rose fermentation recombinant collagen-based fermentation broth as claimed in claim 1 or 2 for the fermentative production of a fermentation product having moisturizing gene expression promoting effect.
7. The use of claim 6, wherein said moisturizing gene comprises the genes AQP-3, CD44, and HAS 1.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202210101822.3A CN114457136A (en) | 2022-01-27 | 2022-01-27 | Fermentation liquid based on rose fermentation recombinant collagen and application thereof |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202210101822.3A CN114457136A (en) | 2022-01-27 | 2022-01-27 | Fermentation liquid based on rose fermentation recombinant collagen and application thereof |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN114457136A true CN114457136A (en) | 2022-05-10 |
Family
ID=81412405
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202210101822.3A Pending CN114457136A (en) | 2022-01-27 | 2022-01-27 | Fermentation liquid based on rose fermentation recombinant collagen and application thereof |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN114457136A (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113667708A (en) * | 2021-08-23 | 2021-11-19 | 山东林森生物制品股份有限公司 | Preparation method of plant intercellular substance |
Citations (19)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102443057A (en) * | 2011-10-26 | 2012-05-09 | 南京理工大学 | A kind of recombinant human collagen and preparation method thereof |
| AU2014200062A1 (en) * | 2010-11-12 | 2014-01-23 | Allergan, Inc. | Metabolized conditioned growth medium and methods of use |
| CN104643086A (en) * | 2015-03-11 | 2015-05-27 | 山东凤凰生物有限公司 | Preparation method of rose ferment |
| US20160022556A1 (en) * | 2013-04-15 | 2016-01-28 | National University Corporation University Of Toyoma | 1,5-anhydro-d-glucitol-containing collagen production accelerator |
| CN106256911A (en) * | 2016-07-22 | 2016-12-28 | 江苏江山聚源生物技术有限公司 | A kind of Pichia sp. fermentation medium being applicable to large-scale production recombination human source collagen protein |
| CN106520869A (en) * | 2016-12-07 | 2017-03-22 | 成都远睿生物技术有限公司 | Fermentation method and fermentation medium for recombinant human III type collagen engineering bacteria |
| CN107033238A (en) * | 2017-05-03 | 2017-08-11 | 成都远睿生物技术有限公司 | The purification process and preparation method of a kind of recombination human source type III collagen |
| CN109893487A (en) * | 2019-04-30 | 2019-06-18 | 上海浅娇生物科技有限公司 | A kind of rice rose fermentation magma and the preparation method and application thereof |
| CN110204608A (en) * | 2019-05-10 | 2019-09-06 | 美尔健（深圳）生物科技有限公司 | One primary yeast fermentation small molecule recombination fibronectin peptide and its preparation method and application |
| CN111671666A (en) * | 2020-06-23 | 2020-09-18 | 湖南美媛本草生物工程有限公司 | Preparation method and application of freeze-dried powder containing multi-effect restoration composition |
| CN111904908A (en) * | 2020-08-19 | 2020-11-10 | 上海怡宝化妆品集团有限公司 | Essence containing rose fermentation liquor and preparation method thereof |
| CN112336677A (en) * | 2020-11-23 | 2021-02-09 | 肖暾 | Collagen moisturizing sunscreen isolation cream and preparation method thereof |
| CN112552393A (en) * | 2020-12-31 | 2021-03-26 | 西安德诺海思医疗科技有限公司 | Recombinant human III-type collagen and pichia pastoris recombinant expression system thereof |
| CN113520905A (en) * | 2021-03-25 | 2021-10-22 | 甘肃天际生物科技有限公司 | Instant collagen microneedle and preparation method thereof |
| CN113789358A (en) * | 2021-08-28 | 2021-12-14 | 西安德诺海思医疗科技有限公司 | Fermentation process for improving production level of recombinant collagen |
| CN113813209A (en) * | 2021-10-26 | 2021-12-21 | 上海百雀羚日用化学有限公司 | Rose petal fermentation liquor and preparation method and application thereof |
| CN114404667A (en) * | 2021-12-07 | 2022-04-29 | 尚诚怡美(成都)生物科技有限公司 | Long-acting recombinant human-derived collagen implant and application thereof |
| CN115558612A (en) * | 2021-07-01 | 2023-01-03 | 华北制药集团新药研究开发有限责任公司 | Recombinant full-length III-type humanized collagen yeast engineering strain and construction method thereof |
| CN115844024A (en) * | 2022-10-12 | 2023-03-28 | 浙江诸暨聚源生物技术有限公司 | Application of recombinant humanized collagen in preparation of edible anti-aging moisturizing and whitening products |
-
2022
- 2022-01-27 CN CN202210101822.3A patent/CN114457136A/en active Pending
Patent Citations (19)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| AU2014200062A1 (en) * | 2010-11-12 | 2014-01-23 | Allergan, Inc. | Metabolized conditioned growth medium and methods of use |
| CN102443057A (en) * | 2011-10-26 | 2012-05-09 | 南京理工大学 | A kind of recombinant human collagen and preparation method thereof |
| US20160022556A1 (en) * | 2013-04-15 | 2016-01-28 | National University Corporation University Of Toyoma | 1,5-anhydro-d-glucitol-containing collagen production accelerator |
| CN104643086A (en) * | 2015-03-11 | 2015-05-27 | 山东凤凰生物有限公司 | Preparation method of rose ferment |
| CN106256911A (en) * | 2016-07-22 | 2016-12-28 | 江苏江山聚源生物技术有限公司 | A kind of Pichia sp. fermentation medium being applicable to large-scale production recombination human source collagen protein |
| CN106520869A (en) * | 2016-12-07 | 2017-03-22 | 成都远睿生物技术有限公司 | Fermentation method and fermentation medium for recombinant human III type collagen engineering bacteria |
| CN107033238A (en) * | 2017-05-03 | 2017-08-11 | 成都远睿生物技术有限公司 | The purification process and preparation method of a kind of recombination human source type III collagen |
| CN109893487A (en) * | 2019-04-30 | 2019-06-18 | 上海浅娇生物科技有限公司 | A kind of rice rose fermentation magma and the preparation method and application thereof |
| CN110204608A (en) * | 2019-05-10 | 2019-09-06 | 美尔健（深圳）生物科技有限公司 | One primary yeast fermentation small molecule recombination fibronectin peptide and its preparation method and application |
| CN111671666A (en) * | 2020-06-23 | 2020-09-18 | 湖南美媛本草生物工程有限公司 | Preparation method and application of freeze-dried powder containing multi-effect restoration composition |
| CN111904908A (en) * | 2020-08-19 | 2020-11-10 | 上海怡宝化妆品集团有限公司 | Essence containing rose fermentation liquor and preparation method thereof |
| CN112336677A (en) * | 2020-11-23 | 2021-02-09 | 肖暾 | Collagen moisturizing sunscreen isolation cream and preparation method thereof |
| CN112552393A (en) * | 2020-12-31 | 2021-03-26 | 西安德诺海思医疗科技有限公司 | Recombinant human III-type collagen and pichia pastoris recombinant expression system thereof |
| CN113520905A (en) * | 2021-03-25 | 2021-10-22 | 甘肃天际生物科技有限公司 | Instant collagen microneedle and preparation method thereof |
| CN115558612A (en) * | 2021-07-01 | 2023-01-03 | 华北制药集团新药研究开发有限责任公司 | Recombinant full-length III-type humanized collagen yeast engineering strain and construction method thereof |
| CN113789358A (en) * | 2021-08-28 | 2021-12-14 | 西安德诺海思医疗科技有限公司 | Fermentation process for improving production level of recombinant collagen |
| CN113813209A (en) * | 2021-10-26 | 2021-12-21 | 上海百雀羚日用化学有限公司 | Rose petal fermentation liquor and preparation method and application thereof |
| CN114404667A (en) * | 2021-12-07 | 2022-04-29 | 尚诚怡美(成都)生物科技有限公司 | Long-acting recombinant human-derived collagen implant and application thereof |
| CN115844024A (en) * | 2022-10-12 | 2023-03-28 | 浙江诸暨聚源生物技术有限公司 | Application of recombinant humanized collagen in preparation of edible anti-aging moisturizing and whitening products |
Non-Patent Citations (2)
| Title |
|---|
| TIANYI WANG等: "Production of recombinant collagen: state of the art and challenges", 《ENGINEERING BIOLOGY》, vol. 1, no. 1, pages 18 - 23, XP006075472, DOI: 10.1049/enb.2017.0003 * |
| 李伟娜等: "毕赤酵母高密度发酵产III型类人胶原蛋白及其胃粘膜修复功能", 《生物工程学报》, no. 4, pages 672 - 682 * |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113667708A (en) * | 2021-08-23 | 2021-11-19 | 山东林森生物制品股份有限公司 | Preparation method of plant intercellular substance |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN106047553B (en) | A kind of strawberry compound health-care fruit wine and its brewing method | |
| KR20220116000A (en) | Saccharopolispora and its application in reducing biogenic amines | |
| CN114606152B (en) | Bacillus bailii, microbial agent and application thereof | |
| CN112795493B (en) | Method for improving yield of fermented cordyceps sinensis mycelia | |
| CN106399423B (en) | A method of trehalose is prepared under the conditions of environment stress using beer waste yeast | |
| CN114457136A (en) | Fermentation liquid based on rose fermentation recombinant collagen and application thereof | |
| CN1317564A (en) | Process for brewing rice vinegar from fermented edible fungas and grains | |
| CN102268384A (en) | Saccharomyces cerevisiae strain and method for preparing blackberry fruit wine by using same | |
| CN113980858A (en) | Lactobacillus plantarum YL399 for producing high-activity tannase and application thereof in preparation of codonopsis pilosula fermented feed | |
| CN104745348B (en) | A kind of preparation method of hami melon flavor beer | |
| CN112391421A (en) | Culture method for improving oil content of saccharomyces cerevisiae by adjusting total salt content of saccharomyces cerevisiae fermentation liquor and high-oil-yield saccharomyces cerevisiae | |
| CN105670936B (en) | A kind of method of Trametes trogii bacterial strain and its application and Pu'er tea processing | |
| EP4349962A1 (en) | Strain hscy 2073, and isolation and screening therefor and use thereof in improving flavor and quality of vinegar | |
| CN113773977B (en) | Yeast strain with low ethanol yield and high aroma yield and application thereof | |
| Nouska et al. | Saccharomyces cerevisiae and Kefir Production using Waste Pomegranate Juice, Molasses, and Whey. | |
| CN114276937B (en) | Method for fermenting paecilomyces hepialid by using Chinese yam as carbon source | |
| RU2599435C1 (en) | Saccharomyces cerevisiae VKPM Y-4066 YEAST STRAIN-ETHYL ALCOHOL PRODUCER | |
| CN104651157B (en) | A kind of production method of yak whey low alcohol beverage | |
| CN109468300B (en) | A kind of preparation method of the culture medium of esterified red yeast rice | |
| CN107156668A (en) | A kind of thin mash fermented method of broad bean paste valve pot type | |
| CN101182443B (en) | Production method of polyporus umbellatus wine | |
| KR20220111148A (en) | Alcohol fermentation enhancer comprising hempseed and alcoholic beverage using the same | |
| CN101020881B (en) | Biological strawberry wine | |
| US20220411836A1 (en) | Methods for extracting anthocyanins to improve urinary health by using cranberries and plant-based lactobacillus which enhance female reproductive health and extracts of thereby | |
| CN114836332B (en) | Pichia kudriavzevii with high tolerance and low isoamyl alcohol yield and application thereof |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination |