CN114453146B - Centrifugal device for gene detection of self-separation solution - Google Patents
Centrifugal device for gene detection of self-separation solution Download PDFInfo
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- CN114453146B CN114453146B CN202210380343.XA CN202210380343A CN114453146B CN 114453146 B CN114453146 B CN 114453146B CN 202210380343 A CN202210380343 A CN 202210380343A CN 114453146 B CN114453146 B CN 114453146B
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- 238000000926 separation method Methods 0.000 title claims abstract description 33
- 108090000623 proteins and genes Proteins 0.000 title claims abstract description 27
- 238000001514 detection method Methods 0.000 title claims abstract description 21
- 239000007788 liquid Substances 0.000 claims abstract description 169
- 238000012360 testing method Methods 0.000 claims abstract description 123
- 230000005540 biological transmission Effects 0.000 claims description 29
- 229920001971 elastomer Polymers 0.000 claims description 10
- 239000005060 rubber Substances 0.000 claims description 10
- 238000007789 sealing Methods 0.000 claims description 9
- 239000000284 extract Substances 0.000 claims description 7
- 238000000429 assembly Methods 0.000 claims description 4
- 230000000712 assembly Effects 0.000 claims description 4
- 238000004887 air purification Methods 0.000 claims 1
- 239000000243 solution Substances 0.000 abstract description 60
- 239000011550 stock solution Substances 0.000 abstract description 13
- 238000000605 extraction Methods 0.000 abstract description 7
- 238000000034 method Methods 0.000 description 19
- 238000005119 centrifugation Methods 0.000 description 14
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- 239000000463 material Substances 0.000 description 2
- 230000010355 oscillation Effects 0.000 description 2
- 230000001502 supplementing effect Effects 0.000 description 2
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- 241000973497 Siphonognathus argyrophanes Species 0.000 description 1
- 230000004075 alteration Effects 0.000 description 1
- 101150036080 at gene Proteins 0.000 description 1
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B04—CENTRIFUGAL APPARATUS OR MACHINES FOR CARRYING-OUT PHYSICAL OR CHEMICAL PROCESSES
- B04B—CENTRIFUGES
- B04B5/00—Other centrifuges
- B04B5/04—Radial chamber apparatus for separating predominantly liquid mixtures, e.g. butyrometers
- B04B5/0407—Radial chamber apparatus for separating predominantly liquid mixtures, e.g. butyrometers for liquids contained in receptacles
- B04B5/0414—Radial chamber apparatus for separating predominantly liquid mixtures, e.g. butyrometers for liquids contained in receptacles comprising test tubes
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B04—CENTRIFUGAL APPARATUS OR MACHINES FOR CARRYING-OUT PHYSICAL OR CHEMICAL PROCESSES
- B04B—CENTRIFUGES
- B04B11/00—Feeding, charging, or discharging bowls
- B04B11/04—Periodical feeding or discharging; Control arrangements therefor
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B04—CENTRIFUGAL APPARATUS OR MACHINES FOR CARRYING-OUT PHYSICAL OR CHEMICAL PROCESSES
- B04B—CENTRIFUGES
- B04B13/00—Control arrangements specially designed for centrifuges; Programme control of centrifuges
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B04—CENTRIFUGAL APPARATUS OR MACHINES FOR CARRYING-OUT PHYSICAL OR CHEMICAL PROCESSES
- B04B—CENTRIFUGES
- B04B7/00—Elements of centrifuges
- B04B7/02—Casings; Lids
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- Centrifugal Separators (AREA)
- Automatic Analysis And Handling Materials Therefor (AREA)
- Sampling And Sample Adjustment (AREA)
Abstract
The invention belongs to the technical field of gene detection, and discloses a centrifugal device for gene detection of self-separation solution, which comprises a machine body, wherein the machine body comprises a working box, one side of the working box is connected with a supporting seat, one end in the working box is provided with a centrifugal cavity, an opening of the centrifugal cavity is positioned at one end far away from the supporting seat, one end of the working box far away from the supporting seat is rotatably provided with a top cover, the top cover can seal the opening of the centrifugal cavity, a plurality of connecting components are arranged in the centrifugal cavity, and each connecting component comprises a fixed block; draw the different degree of depth of solution in the test tube through the liquid outlet port, and then the operating condition in control air cavity and stock solution chamber, when the liquid outlet port was located the shallow solution in the test tube, realized the shallow liquid extraction in the direct stock solution chamber and drain pipe to stock solution intracavity negative pressure through the air cavity.
Description
Technical Field
The invention belongs to the technical field of gene detection, and particularly relates to a centrifugal device for gene detection, which is used for self-separating a solution.
Background
In the process of gene detection, genes in cells need to be extracted and separated first, in the separation process, in order to ensure the separation efficiency, a centrifuge is generally used for separating different substances in a solution, and in the process of extraction and separation, the solution in a test tube needs to be extracted for many times and a new reaction solution is added, so that the required genes can be obtained for experimental adjustment.
Patent application No. CN202020245106.9 discloses a gene detection centrifugation device for self-separation solution, which solves the problem of oscillation in the centrifugation process of a centrifuge, but still needs to separate and extract the solution in a test tube in the centrifuge for many times, so that the efficiency of gene extraction is slow.
Meanwhile, in the process of biological experiments, when the solution in the test tube is extracted and separated, only the solution with a fixed depth can be extracted and separated, but in the process of experiments, because the components of the added reaction reagents are different, the positions of genes in the cells in the test tube after centrifugation are also different, and timely adjustment cannot be made, so that the solution in the test tube still needs to be artificially extracted.
Disclosure of Invention
The invention aims to solve the problems, and provides a centrifugal device for gene detection of self-separation solution, which has the advantages of quickly separating the solution at different depths in a test tube and quickly adding liquid without taking out the test tube.
In order to achieve the purpose, the invention provides the following technical scheme: a centrifugal device for gene detection of self-separation solution comprises a machine body, wherein the machine body comprises a working box, one side of the working box is connected with a supporting seat, one end in the working box is provided with a centrifugal cavity, an opening of the centrifugal cavity is positioned at one end far away from the supporting seat, one end of the working box far away from the supporting seat is rotatably provided with a top cover, and the top cover can seal the opening of the centrifugal cavity;
A plurality of connecting assemblies are arranged in the centrifugal cavity, each connecting assembly comprises a fixed block, the fixed block is fixedly connected to the centrifugal cavity, an expansion plate is arranged on one side of the fixed block in a sliding mode, and a rotating plate is arranged at one end, far away from the fixed block, of the expansion plate in a rotating mode;
a pipeline assembly is connected between the working box and the supporting seat, one end of the pipeline assembly, which is close to the working box, is connected to the connecting assembly, and the pipeline assembly comprises a liquid outlet pipe for separating solution in the test tube, a liquid inlet pipe for allowing the solution to enter the test tube and a gas pipe for assisting liquid separation;
a liquid storage cavity and an air cavity are formed in the supporting seat, one ends of the liquid outlet pipe and the gas pipe, which are far away from the connecting assembly, are respectively communicated with the liquid storage cavity and the air cavity, a communicating cavity is communicated between the air cavity and the liquid storage cavity, a valve controller is fixedly arranged in the communicating cavity, and pressure sensors are fixedly arranged at two ends of the valve controller;
a horizontal rotor for placing a test tube is rotationally arranged in the centrifugal cavity, and the horizontal rotor does not contact with the wall of the centrifugal cavity when rotating;
a telescopic rod is fixedly arranged between the supporting seat and the working box;
when extracting the solution of different aspect in the test tube through controlling the drain pipe, air cavity and stock solution chamber are in different operating condition, and when the drain pipe was located test tube shallow layer solution, the stock solution chamber was direct to be drawed the separation to test tube solution, and when the drain pipe was located the deep solution of test tube, the negative pressure through air cavity pressurization and stock solution chamber realized the liquid rapid segregation to the test tube in.
Compared with the prior art, the invention has the following beneficial effects:
1. the different degree of depth of solution is drawed in the test tube to port through the liquid outlet, and then the operating condition in control air cavity and stock solution chamber, when the liquid outlet port is located the shallow solution of test tube, bleed and realize the negative pressure to stock solution intracavity through the air cavity, directly extract the shallow liquid in the test tube through stock solution chamber and drain pipe, when the port of liquid outlet is located test tube bottom solution, draw the solution in the test tube through air cavity and stock solution chamber this moment, make liquid draw the in-process of separation can be accurate separate the solution of the different degree of depth.
2. After the solution in the test tube is separated, the reagent of the secondary reaction is directly added into the test tube through the liquid inlet pipe, and then the secondary centrifugal reaction is directly carried out under the condition that the test tube is not taken out, so that the time for taking the test tube is reduced, and the centrifugal rate is improved.
3. Cover through the sealed with test tube port and set up three sealed rubber hole, when making things convenient for liquid outlet, inlet and gas transmission mouth to pass through, thereby not influencing the test tube at the sealed centrifugation of centrifugal intracavity, open sealed lid in order to avoid the staff at the in-process mistake of centrifuge work simultaneously, and then set up the telescopic link between work box and supporting seat and be used for reminding the staff.
Drawings
FIG. 1 is a schematic view of the overall apparatus of the present invention;
FIG. 2 is a schematic view of the structure inside the centrifugal chamber according to the present invention;
FIG. 3 is a schematic view of the structure in the other direction in FIG. 2;
FIG. 4 is a schematic view of the arrangement of the telescopic rods according to the present invention;
FIG. 5 is an enlarged view of A in FIG. 2;
FIG. 6 is a schematic cross-sectional view of the support base of the present invention;
FIG. 7 is an enlarged view of B in FIG. 6;
fig. 8 is a schematic view of a connection structure between the pipe assembly and the support base.
Description of the drawings: 1. a body; 11. a supporting seat; 1101. a liquid storage cavity; 1102. an air cavity; 12. a work box; 1201. a centrifugal chamber; 1202. a horizontal rotor; 1203. a power shaft; 13. a top cover; 2. a conduit assembly; 21. a liquid outlet pipe; 2101. a liquid outlet; 22. a liquid inlet pipe; 2201. a liquid inlet; 23. a gas delivery pipe; 2301. a gas transmission port; 3. a connecting assembly; 31. a fixed block; 32. a retractable plate; 33. a rotating plate; 4. a telescopic rod; 5. a communicating cavity; 51. a valve controller.
Detailed Description
The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.
As shown in fig. 1-8, a centrifugal device for gene detection of self-separation solution comprises a machine body 1, the machine body 1 includes a working box 12 for placing a centrifugal sample, a supporting seat 11 is connected to one side of the working box 12, the working box 12 is supported by the supporting seat 11, so that the vibration frequency of the working box 12 during working does not affect the centrifugal operation, a centrifugal cavity 1201 is formed at one end of the working box 12, an opening of the centrifugal cavity 1201 is located at one end far from the supporting seat 11, a top cover 13 is rotatably arranged at one end of the working box 12 far from the supporting seat 11, the top cover 13 can seal the opening of the centrifugal cavity 1201, when a test tube to be centrifuged is placed in the centrifugal cavity 1201, the centrifugal cavity 1201 is sealed by turning over the top cover 13, so that the gas pressure in the centrifugal cavity 1201 is stable during the high-speed rotation centrifugation of liquid, and the centrifuged test tube is not affected by impurities, the safe operation of centrifugation is ensured.
Be equipped with a plurality of coupling assembling 3 that are used for pipeline assembly exit position to retrain in the centrifugal cavity 1201, coupling assembling 3 includes fixed block 31, fixed block 31 fixed connection is on centrifugal cavity 1201, one side slip that fixed block 31 kept away from centrifugal cavity 1201 chamber wall junction is equipped with expansion plate 32, the flexible degree of expansion plate 32 is controlled by the electric conductance rail, be equipped with on the expansion plate 32 and supply rotor plate 33 pivoted recess, the one end rotation that fixed block 31 was kept away from to expansion plate 32 is equipped with rotor plate 33, the rotation power of rotor plate 33 is provided by rotating the motor, rotating motor fixed connection is on expansion plate 32, make liquid outlet 2101 through the rotation of rotor plate 33 simultaneously, inlet 2201 and gas transmission port 2301 rotate to the test tube mouth upside, make things convenient for the liquid outlet 2101, inlet 2201 and gas transmission port 2301 carry out work.
A pipeline component 2 is connected between the working box 12 and the supporting seat 11, one end of the pipeline component 2 close to the working box 12 is connected on the connecting component 3, the pipeline component 2 comprises a liquid outlet pipe 21 for separating solution in a test tube, a liquid inlet pipe 22 for leading the solution into the test tube and a gas pipe 23 for assisting liquid separation, the working box 12 is connected with the supporting seat 11 through the pipeline component 2, and then the solution in the working box 12 is conveniently led into the supporting seat 11 through the pipeline component 2, and simultaneously, in order to avoid the pipeline component 2 from being broken when the working box 12 and the supporting seat 11 generate relative displacement and influencing the normal transportation of the solution, the pipeline materials of the liquid outlet pipe 21, the liquid inlet pipe 22 and the gas pipe 23 are provided with rubber elastic pipes, after the working box 12 generates displacement, the liquid outlet pipe 21, the liquid inlet pipe 22 and the gas pipe 23 generate extension, so that the two ends of the pipeline component 2 are always connected on the working box 12 and the supporting seat 11, it should be noted that the displacement between the work box 12 and the support base 11 is smaller than the extension of the duct assembly 2.
A liquid storage cavity 1101 and an air cavity 1102 are arranged in the supporting seat 11, liquid separated from the test tube in the test tube is placed through the liquid storage cavity 1101, air to be discharged into the test tube is compressed and stored through the air cavity 1102, one ends, far away from the connecting assembly 3, of the liquid outlet pipe 21 and the air inlet pipe 23 are respectively communicated with the liquid storage cavity 1101 and the air cavity 1102, air in the air cavity 1102 is led into the test tube through the air inlet pipe 23, meanwhile, the air pressure at the upper end in the test tube is increased through the input air, liquid at the bottom of the test tube flows into the liquid storage cavity 1101 through the liquid outlet pipe 21 to be placed, meanwhile, in order to improve the separation rate and the separation diversity of the solution in the test tube, pressure difference exists between the pressure in the liquid storage cavity 1101 and the pressure in the test tube, the moving rate of the solution in the test tube into the liquid storage cavity 1101 is improved, a communication cavity 5 is further communicated between the air cavity 1102 and the liquid storage cavity 1101, wherein the position of the communication cavity 5 in the liquid storage cavity 1101 does not influence the placement of the liquid storage cavity 1101, the communicating cavity 5 is internally and fixedly provided with a valve controller 51, the liquid storage cavity 1101 is separated from the air cavity 1102 through the valve controller 51, so that the two cavities are independent individuals respectively when in work, meanwhile, the two ends of the valve controller 51 are fixedly provided with pressure sensors, the pressure in the liquid storage cavity 1101 is detected through the pressure sensor arranged on one side of the liquid storage cavity 1101, the pressure difference value is enough to enable the liquid to completely enter the liquid storage cavity 1101 through the liquid outlet pipe 21 when the liquid is sucked by the liquid storage cavity 1101, meanwhile, the pressure in the air cavity 1102 is detected through the pressure sensor arranged in the air cavity 1102, when the pressure in the air cavity 1102 is low, the gas content in the air cavity 1102 is low, the requirement for supplementing into a test tube is not met, and then the data is uploaded to the control terminal through the pressure sensor, so that the control terminal controls the air extractor to timely supplement the gas in the air cavity 1102, and through the gas pressure of both sides, the switch of control valve controller 51, when needing stock solution chamber 1101 to absorb the liquid in the test tube alone, control valve controller 51 opens for the gas in stock solution chamber 1101 is extracted to the air exhaust in air cavity 1102, when pressure sensor in stock solution chamber 1101 detects the pressure in the intracavity and can extract the test tube solution, control valve controller 51 is closed.
In the centrifugal experiment, when the separation solution on the upper layer of the test tube needs to be extracted, the liquid outlet pipe 21 extends into the upper layer solution in the test tube, at the moment, the gas conveying pipe 23 does not pressurize the interior of the test tube, and the liquid on the upper layer is adsorbed out of the test tube through the liquid outlet pipe 21 by using the pressure difference in the liquid storage cavity 1101; when the separation solution of test tube lower floor is extracted as required, make drain pipe 21 stretch into in the solution of test tube bottom, gas-supply pipe 23 is located the cavity department of test tube upside this moment, thereby utilize the pressurization of gas-supply pipe 23 in to the test tube, make liquid receive decurrent oppression, utilize the negative pressure between liquid storage chamber 1101 and the drain pipe 21 simultaneously, the realization is to the liquid extraction of test tube downside, improve the separation rate of liquid, it is required to explain, when drain pipe 21 and gas-supply pipe 23 do not work, the valve between drain pipe 21 and liquid storage chamber 1101 and gas-supply pipe 23 and air cavity 1102 is the closed state, after the port of drain pipe 21 and gas-supply pipe 23 reachd the operating position, control flap opens, make drain pipe 21 and gas-supply pipe 23 begin work.
Meanwhile, a control terminal is connected to the outer end of the machine body 1, so that the control terminal controls the operation of the valve, the on-off of the valve controller 51, the deflection of the rotating plate 33 and the like.
In order to facilitate the separation and extraction of the solution in the test tube and accelerate the rate of the solution separation, a horizontal rotor 1202 for placing the test tube is rotatably arranged in the centrifugal cavity 1201, and meanwhile, the horizontal rotor 1202 does not contact with the wall of the centrifugal cavity 1201 when rotating, so that the rotation stability of the horizontal rotor 1202 is ensured in the working process of the horizontal rotor 1202, and large-amplitude oscillation cannot occur.
In this embodiment, the number of ports for placing test tubes on the horizontal rotor 1202 is six, and the number of the connecting assemblies 3 is the same as that of the horizontal rotor 1202, and is also six, and the positions of the connecting assemblies 3 are located on the upper side of the horizontal rotor 1202.
A power shaft 1203 is rotatably arranged on the inner wall of one side of the centrifugal cavity 1201 close to the supporting seat 11, the rotation of the power shaft 1203 is provided by a servo motor, the servo motor is fixedly connected in the box body of the working box 12, a through hole is arranged in the horizontal rotor 1202, a clamping component which can be connected with the power shaft 1203 is fixedly arranged in the through hole of the horizontal rotor 1202, in this embodiment, the clamping component is preferably a rotating chuck, i.e., by relative sliding between the horizontal rotor 1202 and the power shaft 1203, the spin chuck is unscrewed to interfere with the power shaft 1203, and in order to avoid loosening of the spin chuck during operation of the power shaft 1203, and the direction of the rotating chuck screwing out is the same as the rotating direction of the power shaft 1203, the through hole of the horizontal rotor 1202 can be installed on the power shaft 1203, meanwhile, in order to facilitate the clamping of the power shaft 1203 by the clamping assembly, a groove capable of being clamped is formed in the power shaft 1203.
Fixed telescopic link 4 that is equipped with between supporting seat 11 and the work box 12, supporting seat 11 supports with ground contact, the end face contaction of work box 12 and supporting seat 11, after work box 12 prepares work and accomplishes, through telescopic link 4 with work box 12 lifting, avoid work box 12 to be opened by the staff mistake when centrifugal work goes on, cause the condition of centrifugation failure to take place, simultaneously through the interval between direct observation work box 12 and the supporting seat 11, whether the centrifugal condition is accomplished to work box 12 that just can audio-visually observes.
In order to separate the solution in the test tube conveniently, the extension length of the pipeline assembly 2 is reduced, a liquid outlet 2101, a liquid inlet 2201 and a gas transmission port 2301 are respectively and fixedly arranged on the rotating plate 33, one ends of the liquid outlet 21, the liquid inlet 22 and the gas transmission port 23, which are far away from the supporting seat 11, penetrate through the box body of the working box 12 and are respectively communicated with the liquid outlet 2101, the liquid inlet 2201 and the gas transmission port 2301, the liquid outlet, the liquid inlet 2201 and the gas transmission port 2301 are respectively equivalent to the extension ends of the liquid outlet 21, the liquid inlet 22 and the gas transmission port 23, and the liquid outlet 2101, the liquid inlet 2201 and the gas transmission port 2301 can extend into the test tube on the horizontal rotor 1202 to work after being turned.
Meanwhile, in order to avoid that the lengths of the liquid outlet 2101, the liquid inlet 2201 and the gas transmission port 2301 in the centrifugal cavity 1201 are too long and influence the normal turning of the rotating plate 33, and further, the ends of the liquid outlet 2101, the liquid inlet 2201 and the gas transmission port 2301 far away from the rotating plate 33 are movable telescopic ends, the movable telescopic ends can drive the liquid outlet 2101, the liquid inlet 2201 and the gas transmission port 2301 to extend, and meanwhile, the communication condition of the liquid outlet 2101, the liquid inlet 2201 and the gas transmission port 2301 cannot be influenced in the extension process of the movable telescopic ends, in the embodiment, the telescopic mode of the movable telescopic ends is preferably the telescopic mode of an electric guide rail, so that the separated solution in the test tube cannot be influenced in the extension process, and meanwhile, in order to enable the liquid outlet 2101, the liquid inlet 2201 and the gas transmission port 2301 to work in the test tube more conveniently, the extension end of the liquid outlet 2101 can extend into the bottom of the test tube to the longest extent, and the extension end of the liquid inlet 2201 can extend into the middle part to the longest extent into the middle part of the test tube, the extended end of the air transfer port 2301 is longest to be able to reach the upper part of the test tube.
Because the liquid is centrifuged in the test tube, in order to avoid splashing of the liquid, the test tube needs to be kept in a sealed state, and the rotating plate 33 cannot directly extend the liquid outlet 2101, the liquid inlet 2201 and the gas transmission port 2301 into the test tube after rotation is completed, and three holes are formed in a sealing plug used on the test tube, wherein the three holes correspond to the liquid outlet 2101, the liquid inlet 2201 and the gas transmission port 2301 respectively, and the sealing form of the holes is formed by mutual collision of a plurality of flexible rubbers, when an object extends into the test tube, the object firstly collides on the flexible rubbers, after the collision force is increased, the object extends into the test tube through the rubbers, and simultaneously after the object is moved out of the sealing plug, the plurality of flexible rubbers recover the mutually collided state, the tube cavity of the test tube is kept in a sealed state, and when the rotating plate 33 drives the liquid outlet, the liquid inlet 2201 and the gas transmission port 2301 to rotate to the upper end of the test tube, stretch into three mouth through the flexible end of activity and carry out work to the test tube in, can not influence the leakproofness of test tube in centrifugal process simultaneously.
In-process at gene detection, the solution centrifugation needs to add multiple solution and separates the material of difference on to the cell, so at the in-process that the gene drawed, need add multiunit reagent respectively and carry out the multiunit centrifugation, and then for the time that reduces the test tube of taking after the centrifugation is accomplished, the one end that sets up feed liquor pipe 22 and keep away from work box 12 passes the outer end intercommunication of supporting seat 11 with supporting seat 11, make the staff directly get into to the test tube inside through feed liquor pipe 22 and inlet 2201, the fixed sealing plug that is equipped with is served to the intercommunication at feed liquor pipe 22 simultaneously, avoid feed liquor pipe 22 to have impurity to get into when not using, and then after influence reagent passes through feed liquor pipe 22 and gets into the test tube, influence centrifugal result.
In order to conveniently supplement the gas in the gas cavity 1102, furthermore, the side wall of the gas cavity 1102 is provided with a vent hole, an open-close plate is arranged in the vent hole, when the gas in the gas cavity 1102 is in a sufficient state, the open-close plate is in a closed state, so that the gas cavity 1102 is in an open state, the gas in the gas cavity 1102 is conveniently supplemented, when a pressure sensor in the gas cavity 1102 detects that the pressure in the gas cavity 1102 is low, data is uploaded to a control terminal, so that the control terminal controls the open-close plate to be opened, the gas in the gas cavity 1102 is stored, and when the gas is directly sucked into the gas cavity 1102, impurities in the gas are easily adsorbed into the gas inlet cavity 1102, so that when the gas is discharged through the gas pipe 23, the impurities are easily introduced into a test tube, and a gas purifying device is arranged on one side, close to the vent hole, of the gas cavity 1102, impurities in the gas are filtered so that the gas stored in the gas chamber 1102 is purified.
Initially, the top cover 13 seals the centrifugal cavity 1201, the telescopic rod 4 is in a contracted state, the valve controller 51 is in a closed state, the liquid outlet 2101, the liquid inlet 2201 and the gas delivery port 2301 do not rotate on the rotating plate 33 and do not change in length, the horizontal rotor 1202 and the power shaft 1203 are in a separated state, and the telescopic plate 32 is accommodated in the fixed block 31.
During operation, the top cover 13 is turned over to open the centrifugal cavity 1201, the horizontal rotor 1202 is placed on the power shaft 1203, the power shaft 1203 is maintained not to rotate, the horizontal rotor 1202 and the power shaft 1203 rotate relatively, the clamping component on the horizontal rotor 1202 is unfolded to realize position fixing with the power shaft 1203, the stability of the horizontal rotor 1202 in the centrifugal process is ensured, a test tube with a solution stored therein is placed in the placing cavity of the horizontal rotor 1202, the top cover 13 is reset to realize resealing in the centrifugal cavity 1201, meanwhile, the telescopic rod 4 is operated on the premise that the connection between the horizontal rotor 1202 and the power shaft 1203 is stable, namely after the horizontal rotor 1202 and the power shaft 1203 are stably connected, the control terminal can send a control command to operate the telescopic rod 4, if the connection is not stable, the top cover 13 needs to be opened again to adjust the constraint position of the horizontal rotor 1202, after connecting stably, regulate and control the extension of telescopic link 4 through control terminal, and then drive work box 12 and rise, simultaneously at the in-process that work box 12 riseed, drive pipeline assembly 2 and take place the elastic extension, rise to the maximum height back along with telescopic link 4 when work box 12, telescopic link 4 stops the extension, and then make servo motor begin to work through control terminal, it rotates to drive power shaft 1203, thereby it begins to rotate to drive horizontal rotor 1202, the realization is separated the solution composition in the test tube of placing on horizontal rotor 1202.
After the centrifugation is finished, when the horizontal rotor 1202 stops rotating, the port of the placing cavity corresponds to the position of the connecting assembly 3, the extension length of the expansion plate 32 on the fixed block 31 is regulated and controlled through the electric guide rail, the expansion plate 32 is contracted in the fixed block 31 through the expansion plate 32, so that test tubes of different types cannot collide with the expansion plate 32 when rotating on the horizontal rotor 1202, the expansion plate 32 drives the rotation plate 33 to move, the rotation plate 33 is further moved to the position that the liquid outlet 2101, the liquid inlet 2201 and the gas transmission port 2301 are just positioned at the upper end of the test tube port after being turned over, meanwhile, when the test tubes are centrifuged in the centrifugation cavity 1201, in order to avoid the sputtering of solution in the test tubes, the test tubes need to be always kept in a sealed state, and therefore, through the three rubber sealing ports arranged on the sealing covers at the upper ends of the test tubes, after the rotation of the rotation plate 33 is finished, the liquid outlet 2101, the liquid inlet 2201 and the gas transmission port 2301 are elongated, because the three rubber sealing ports on the test tubes correspond to the positions of the three ports, and then along with liquid outlet 2101 and gas transmission mouth 2301's extension, inlet 2201 is located sealed end position unchangeable, passes sealed rubber mouth on the test tube and enters into test tube cavity inside, and gas transmission mouth 2301 is located the cavity department in the test tube simultaneously, owing to in the gene detection process, the cell that needs to draw distributes in different positions in the solution of difference, needs draw respectively according to the solution of actual demand condition to the different degree of depth:
When the separation solution on the upper layer of the test tube needs to be extracted, namely, the liquid storage cavity 1101 needs to absorb the liquid in the test tube independently, so that the liquid outlet 2101 extends into the upper layer solution in the test tube, at the moment, the elongation degree of the liquid outlet 2101 and the data of the air pressure sensor positioned in the liquid storage cavity 1101 are uploaded to the control terminal, so that the valve controller 51 is controlled to be opened through the control terminal, the air suction device in the air cavity 1102 extracts the gas in the liquid storage cavity 1101, when the pressure sensor in the liquid storage cavity 1101 detects that the pressure in the cavity can extract the test tube solution, the valve controller 51 is controlled to be closed, meanwhile, the gas transmission port 2301 does not pressurize the interior of the test tube, and the liquid positioned on the upper layer is adsorbed out of the test tube through the liquid outlet tube 21 by utilizing the pressure difference between the liquid storage cavity 1101 and the test tube; when the separated solution on the lower layer of the test tube needs to be extracted, the liquid outlet tube 21 extends into the solution at the bottom of the test tube, the gas pipe 23 is located in the cavity on the upper side of the test tube, the gas pipe 23 is used for pressurizing the inside of the test tube, liquid is under downward pressure, the valve controller 51 between the liquid storage cavity 1101 and the gas cavity 1102 is in an open state at the moment, gas in the liquid storage cavity 1101 enters the gas cavity 1102 through the communicating cavity 5 for storage, negative pressure is formed between the liquid storage cavity 1101 and the test tube, the solution on the bottom layer of the test tube is drained, further, along with gas input of the gas conveying port 2301, liquid on the bottom layer of the test tube enters the liquid outlet tube 21, further along with flowing of the liquid in the liquid outlet tube 21, the liquid is fed back to the valve controller 51 through the control terminal, the valve controller 51 is reduced along with shortening of the distance from the liquid to the liquid storage cavity 1101, after the liquid flows into the liquid storage cavity 1101, the valve controller 51 is completely closed, meanwhile, the air cavity 1102 continuously pressurizes the inside of the test tube through the air pipe 23, so that the solution in the test tube is separated, the pressure in the liquid storage cavity 1101 is detected through the pressure sensor arranged on one side of the liquid storage cavity 1101, the pressure difference value is convenient to enable the liquid to completely enter the liquid storage cavity 1101 through the liquid outlet pipe 21 when the liquid storage cavity 1101 sucks the liquid, the pressure in the air cavity 1102 is detected through the pressure sensor arranged in the air cavity 1102, when the pressure in the air cavity 1102 is low, the content of the gas in the air cavity 1102 is low, the requirement of supplementing the gas into the test tube is not met, then the data is uploaded to the control terminal through the pressure sensor, so that the control terminal controls the air suction device to timely supplement the gas in the air cavity 1102, and controls the opening and closing of the valve controller 51 through the gas pressures on the two sides to realize the liquid extraction on the lower side of the test tube, the separation rate of the liquid is increased.
After solution separation in the test tube, make liquid outlet 2101 and gas transmission port 2301 shift out in the test tube, simultaneous control inlet 2201 extension, stretch into to the inside of the test tube, and then the staff opens the sealing plug that is located on supporting seat 11, and then pour into liquid to the inside of test tube through the open end of feed liquor pipe 22, install the drawing liquid pump on inlet 2201 simultaneously, make the liquid in the feed liquor pipe 22 can get into to the test tube completely, and then extract inlet 2201 and make the test tube be in encapsulated situation again, and then the secondary centrifugation, accomplish until the gene extraction, reduce the time that consumes in gene centrifugation process.
It should be noted that, in this document, relational terms such as first and second, and the like are used solely to distinguish one entity or action from another entity or action without necessarily requiring or implying any actual such relationship or order between such entities or actions. Also, the terms "comprises," "comprising," or any other variation thereof, are intended to cover a non-exclusive inclusion, such that a process, method, article, or apparatus that comprises a list of elements does not include only those elements but may include other elements not expressly listed or inherent to such process, method, article, or apparatus.
Although embodiments of the present invention have been shown and described, it will be appreciated by those skilled in the art that various changes, modifications, substitutions and alterations can be made in these embodiments without departing from the principles and spirit of the invention, the scope of which is defined in the appended claims and their equivalents.
Claims (8)
1. A centrifugal device for gene detection of self-separation solution comprises a machine body, and is characterized in that: the machine body comprises a working box, one side of the working box is connected with a supporting seat, one end in the working box is provided with a centrifugal cavity, an opening of the centrifugal cavity is positioned at one end far away from the supporting seat, one end of the working box far away from the supporting seat is rotatably provided with a top cover, and the top cover can seal the opening of the centrifugal cavity;
a plurality of connecting assemblies are arranged in the centrifugal cavity, each connecting assembly comprises a fixed block, the fixed block is fixedly connected to the centrifugal cavity, an expansion plate is arranged on one side of the fixed block in a sliding mode, and a rotating plate is arranged at one end, far away from the fixed block, of the expansion plate in a rotating mode;
a pipeline assembly is connected between the working box and the supporting seat, one end of the pipeline assembly, which is close to the working box, is connected to the connecting assembly, and the pipeline assembly comprises a liquid outlet pipe for separating solution in the test tube, a liquid inlet pipe for allowing the solution to enter the test tube and a gas conveying pipe for assisting liquid separation;
A liquid storage cavity and an air cavity are formed in the supporting seat, one ends, far away from the connecting assembly, of the liquid outlet pipe and the gas conveying pipe are respectively communicated with the liquid storage cavity and the air cavity, a communicating cavity is formed between the air cavity and the liquid storage cavity in a communicating mode, a valve controller is fixedly arranged in the communicating cavity, and pressure sensors are fixedly arranged at two ends of the valve controller;
a horizontal rotor for placing a test tube is rotationally arranged in the centrifugal cavity, and the horizontal rotor is not in contact with the wall of the centrifugal cavity when rotating;
a telescopic rod is fixedly arranged between the supporting seat and the working box;
when the liquid outlet pipe is controlled to extract solutions of different layers in a test tube, the air cavity and the liquid storage cavity are in different working states, when the liquid outlet pipe is located in the shallow solution of the test tube, the liquid storage cavity directly extracts and separates the solution of the test tube, and when the liquid outlet pipe is located in the deep solution of the test tube, the liquid in the test tube is rapidly separated by pressurizing the air cavity and negative pressure of the liquid storage cavity.
2. The centrifugal apparatus for gene detection from a separation solution according to claim 1, characterized in that: the rotating plate is fixedly provided with a liquid outlet, a liquid inlet and a gas transmission port respectively, one end of the liquid outlet, the liquid inlet and the gas transmission pipe, which is far away from the supporting seat, penetrates through the working box body and is communicated with the liquid outlet, the liquid inlet and the gas transmission port respectively, and the liquid outlet, the liquid inlet and the gas transmission port can stretch into the test tube on the horizontal rotor after being overturned.
3. The centrifugal apparatus for gene detection from a separation solution according to claim 2, characterized in that: the liquid outlet, the liquid inlet and one end of the gas transmission port, which is far away from the rotating plate, are movable telescopic ends.
4. The centrifugal apparatus for gene detection from a separation solution according to claim 1, characterized in that: the liquid outlet pipe, the liquid inlet pipe and the gas conveying pipe are made of rubber elastic pipes.
5. The centrifugal apparatus for gene detection from a separation solution according to claim 1, characterized in that: the centrifugal rotor is characterized in that a power shaft is rotatably arranged on the inner wall of one side, close to the supporting seat, of the centrifugal cavity, a through hole penetrates through the horizontal rotor, and the through hole of the horizontal rotor can be arranged on the power shaft.
6. The centrifugal apparatus for gene detection from a separation solution according to claim 5, characterized in that: and a clamping component which can be connected with the power shaft is fixedly arranged in the through hole of the horizontal rotor.
7. The centrifugal apparatus for gene detection from a separation solution according to claim 1, characterized in that: one end of the liquid inlet pipe, which is far away from the working box, penetrates through the supporting seat and is communicated with the outer end of the supporting seat, and a sealing plug is fixedly arranged on the communicating end of the liquid inlet pipe.
8. The centrifugal apparatus for gene detection from a separation solution according to claim 1, characterized in that: the side wall of the air cavity is provided with an air vent, an opening plate is arranged in the air vent, and one side of the air cavity close to the air vent is provided with an air purification device.
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| CN119076240B (en) * | 2024-11-07 | 2025-02-07 | 浙江丽水凯达环保设备有限公司 | Automatic layering loading device for centrifugal machine |
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