CN114367144B - In-situ multichannel water enrichment filtering and fixing device and method - Google Patents
In-situ multichannel water enrichment filtering and fixing device and method Download PDFInfo
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- CN114367144B CN114367144B CN202210083218.2A CN202210083218A CN114367144B CN 114367144 B CN114367144 B CN 114367144B CN 202210083218 A CN202210083218 A CN 202210083218A CN 114367144 B CN114367144 B CN 114367144B
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- 238000001914 filtration Methods 0.000 title claims abstract description 99
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 title claims abstract description 71
- 238000011065 in-situ storage Methods 0.000 title claims abstract description 27
- 238000000034 method Methods 0.000 title claims abstract description 15
- 230000002572 peristaltic effect Effects 0.000 claims abstract description 101
- 239000007788 liquid Substances 0.000 claims abstract description 81
- 238000005070 sampling Methods 0.000 claims abstract description 10
- 239000012528 membrane Substances 0.000 claims abstract description 9
- 239000012535 impurity Substances 0.000 claims description 18
- 238000006243 chemical reaction Methods 0.000 claims description 12
- 238000004140 cleaning Methods 0.000 claims description 10
- 238000004891 communication Methods 0.000 claims description 9
- 238000003860 storage Methods 0.000 claims description 5
- 244000005700 microbiome Species 0.000 abstract description 9
- 238000002347 injection Methods 0.000 abstract description 4
- 239000007924 injection Substances 0.000 abstract description 4
- 230000000694 effects Effects 0.000 abstract description 2
- 238000007789 sealing Methods 0.000 abstract 1
- 238000011160 research Methods 0.000 description 4
- 230000008878 coupling Effects 0.000 description 3
- 238000010168 coupling process Methods 0.000 description 3
- 238000005859 coupling reaction Methods 0.000 description 3
- 238000010586 diagram Methods 0.000 description 2
- 239000000834 fixative Substances 0.000 description 2
- 239000013535 sea water Substances 0.000 description 2
- 229910001220 stainless steel Inorganic materials 0.000 description 2
- 239000010935 stainless steel Substances 0.000 description 2
- 239000007900 aqueous suspension Substances 0.000 description 1
- 230000003139 buffering effect Effects 0.000 description 1
- 238000005260 corrosion Methods 0.000 description 1
- 230000007797 corrosion Effects 0.000 description 1
- 238000013461 design Methods 0.000 description 1
- 239000012530 fluid Substances 0.000 description 1
- 238000005374 membrane filtration Methods 0.000 description 1
- 230000008092 positive effect Effects 0.000 description 1
- 238000012827 research and development Methods 0.000 description 1
- 238000000967 suction filtration Methods 0.000 description 1
Classifications
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01D—SEPARATION
- B01D36/00—Filter circuits or combinations of filters with other separating devices
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01D—SEPARATION
- B01D35/00—Filtering devices having features not specifically covered by groups B01D24/00 - B01D33/00, or for applications not specifically covered by groups B01D24/00 - B01D33/00; Auxiliary devices for filtration; Filter housing constructions
- B01D35/02—Filters adapted for location in special places, e.g. pipe-lines, pumps, stop-cocks
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F1/00—Treatment of water, waste water, or sewage
- C02F1/001—Processes for the treatment of water whereby the filtration technique is of importance
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F1/00—Treatment of water, waste water, or sewage
- C02F1/44—Treatment of water, waste water, or sewage by dialysis, osmosis or reverse osmosis
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/02—Devices for withdrawing samples
- G01N1/10—Devices for withdrawing samples in the liquid or fluent state
- G01N1/14—Suction devices, e.g. pumps; Ejector devices
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
- G01N1/40—Concentrating samples
- G01N1/4077—Concentrating samples by other techniques involving separation of suspended solids
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F2103/00—Nature of the water, waste water, sewage or sludge to be treated
- C02F2103/08—Seawater, e.g. for desalination
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/02—Devices for withdrawing samples
- G01N1/10—Devices for withdrawing samples in the liquid or fluent state
- G01N2001/1031—Sampling from special places
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
- G01N1/40—Concentrating samples
- G01N1/4077—Concentrating samples by other techniques involving separation of suspended solids
- G01N2001/4088—Concentrating samples by other techniques involving separation of suspended solids filtration
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- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Hydrology & Water Resources (AREA)
- General Physics & Mathematics (AREA)
- Pathology (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Physics & Mathematics (AREA)
- Immunology (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Environmental & Geological Engineering (AREA)
- Water Supply & Treatment (AREA)
- Organic Chemistry (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Separation Using Semi-Permeable Membranes (AREA)
Abstract
The invention belongs to the field of deep sea in-situ water enrichment filtration and fixation, in particular to an in-situ multichannel water enrichment filtration and fixation device and method. The invention meets the enrichment and filtration requirements of water microorganisms, and uses a peristaltic pump multi-pump head mode and a multi-channel rotary valve body to complete high-flux filtration and various base liquid injection of the water; the invention has the advantages of multiple sampling channels, good fixing effect, flexible and stable work, capability of quickly and effectively acquiring the filtering membrane sample and in-situ fixing, and the connectivity and the sealing performance of the filtering membrane sample are finished by using a single valve body, so that a large number of external structures and additional valve bodies are reduced, and the filtering membrane sample has great practicability.
Description
Technical Field
The invention belongs to the field of deep sea in-situ water enrichment filtration and fixation, and particularly relates to an in-situ multichannel water enrichment filtration and fixation device and method.
Background
The high-flux, rapid and accurate enrichment and filtration of the water body filtration sample is a primary condition for developing the research of marine water body microorganisms, so that the research and development of related equipment are developed by the main marine research institutions in China. The existing underwater equipment comprises direct-taking type water body equipment, in-situ enrichment filtration type sampling equipment, in-situ enrichment fixed type equipment and the like, and is various in variety, and comprises a fidelity pressure-maintaining sampler, a deep-sea microorganism uncapped-type culture cabin, a deep-sea microorganism in-situ enrichment device, an eight-channel deep-sea in-situ microorganism culture device, a grid-plate type microorganism culture device, a large-volume water sample suction filtration sampling system of Mclane company, a deep-sea hot liquid nozzle microorganism filtration sampling device, a multistage membrane filtration pressure-maintaining sampler, an in-situ microorganism filtration fixing device developed by deep-sea science and engineering research institute of China academy of sciences, a water suspension in-situ time-sharing subsampling and filtration system (CN 113069807A) and the like.
In summary, the enrichment filtration system is more involved, but the number of the enrichment filtration and fixing systems in the deep water area is very small, the injection of the multi-channel valve and the fixing liquid is involved, the common use method is that the multi-channel solenoid valve is used for completing the fluid on-off of the multi-channel, the injection of the fixing liquid is completed by using an additional electric pump, but the injection is not completed by using the same pump and the rotary valve.
Disclosure of Invention
Aiming at the characteristics of a peristaltic pump and a rotary valve body, the invention aims to provide an in-situ multichannel water enrichment filtering and fixing device and method.
The aim of the invention is realized by the following technical scheme:
The invention relates to a structure of an enrichment filtering and fixing device, which comprises a peristaltic pump, a multi-channel rotary valve and an enrichment filtering component, wherein the peristaltic pump is provided with at least two peristaltic pump channels, an inlet of one peristaltic pump channel is connected with an impurity filter through an external input channel, an inlet of the other peristaltic pump channel is connected with a fixing liquid, an input channel is arranged between the multi-channel rotary valve and the peristaltic pump, one end of the input channel is communicated with a rotary valve input port of the multi-channel rotary valve, the other end of the input channel is divided into branch channels with the same quantity as the peristaltic pump channels, and each branch channel is connected with an outlet of one peristaltic pump channel; the multi-channel rotary valve is provided with a plurality of rotary valve output ports, each rotary valve output port rotates along with the multi-channel rotary valve, one end of each rotary valve output port is respectively communicated with the rotary valve input port in the rotating process, the other end of each rotary valve output port is respectively connected with a filtering channel, and each filtering channel is provided with an enrichment filtering component; the enrichment filter assembly comprises a filter channel, a filter and a buffer tank, wherein one end of the filter channel is connected with a rotary valve output port on the multi-channel rotary valve, and the other end of the filter channel is sequentially connected with the filter and the buffer tank.
Wherein: the peristaltic pump and the multi-channel rotary valve are respectively connected with a control and power supply system, and the control and power supply system respectively controls and supplies power to the peristaltic pump and the multi-channel rotary valve to finish forward and reverse conversion of the multi-channel rotary valve and forward and reverse movement of the peristaltic pump.
Each branch channel is a parallel channel, and each branch channel is provided with a one-way valve which can only feed liquid to the multi-channel rotary valve.
Each enrichment filter assembly is further provided with a vertical micro water meter and a one-way valve, the liquid inlet end of the buffer tank is connected with the liquid outlet end of the filter, and the liquid outlet end of the buffer tank is sequentially connected with the vertical micro water meter and the one-way valve.
The invention relates to an enrichment filtration and fixing device, which comprises a peristaltic pump, a multi-channel rotary valve, two-position three-way valves and enrichment filtration components, wherein an output channel is arranged between the multi-channel rotary valve and the peristaltic pump, one end of the output channel is communicated with a rotary valve output port of the multi-channel rotary valve, the other end of the output channel is communicated with a peristaltic pump inlet of the peristaltic pump, the multi-channel rotary valve is provided with a plurality of rotary valve input ports, each rotary valve input port rotates along with the multi-channel rotary valve, one end of each rotary valve input port is respectively communicated with a rotary valve output port in the rotation process, the other end of one rotary valve input port is connected with a fixing liquid, the other ends of other rotary valve input ports are respectively connected with filtration channels, and each filtration channel is provided with an enrichment filtration component; the enrichment filtering component comprises an impurity filter, a filter input channel, a buffer tank, a filter, a tee joint and a filter output channel, wherein one end of the filter output channel is connected with the other end of the rotary valve input port, the other end of the filter output channel is connected with one interface of the tee joint, a second interface of the tee joint is sequentially connected with the filter and the buffer tank, the impurity filter is connected with the buffer tank through the filter input channel, and a third interface of the tee joint is connected with a drainage pipeline; the peristaltic pump outlet of the peristaltic pump is connected with the two-position three-way valve inlet of the two-position three-way valve, the normally open outlet of the two-position three-way valve is connected with a water outlet through a pipeline, the normally closed outlet of the two-position three-way valve is connected with the liquid inlet end of a multi-channel connector, the multi-channel connector is provided with connectors the same as the enrichment filter assembly in number, and each connector is connected to a filter input channel in the enrichment filter assembly through a fixed liquid channel.
The peristaltic pump, the multi-channel rotary valve and the two-position three-way valve are respectively connected with a control and power supply system, and the control and power supply system respectively controls and supplies power to the peristaltic pump, the multi-channel rotary valve and the two-position three-way valve to finish forward and reverse conversion of the multi-channel rotary valve, forward and reverse movement of the peristaltic pump and conversion of a normally-open outlet and a normally-closed outlet of the two-position three-way valve.
The filter output channel between one interface of the tee joint and the rotary valve input port is provided with a vertical micro water meter, the filter input channel between the impurity filter and the buffer box is provided with a one-way valve which can only feed liquid into the buffer box, and the fixed liquid channel is connected between the one-way valve and the buffer box.
The multi-channel rotary valve comprises a multi-channel valve core, wherein a plurality of rotary valve output ports are uniformly arranged on the multi-channel valve core of the multi-channel rotary valve along the circumferential direction or a plurality of rotary valve input ports are uniformly arranged on the multi-channel valve core along the circumferential direction, the multi-channel rotary valve is provided with a rotary valve input port or a rotary valve output port, the multi-channel valve core is connected with the output end of an underwater motor through an underwater coupling, the underwater motor drives the multi-channel valve core to rotate and perform conversion of each channel on the multi-channel rotary valve, and a multi-channel rotary valve communication port of the multi-channel rotary valve is connected with a control and power supply system through a multi-channel rotary valve control cable, and the multi-channel rotary valve communication port is a control and power supply port of the underwater motor.
The invention relates to an in-situ multichannel water enrichment filtering and fixing method, which adopts the in-situ multichannel water enrichment filtering and fixing device and comprises the following steps:
Step one, integrally disassembling and cleaning the shore base end; the whole device is disassembled and then cleaned;
Step two, assembling after cleaning, and filling the fixing liquid in an initial state;
Thirdly, lowering the device to the required water depth by utilizing a ship mooring rope, and carrying out enrichment filtration and fixing work; the multi-channel rotary valve is used for communicating the rotary valve input port with a rotary valve output port, and opening a peristaltic pump channel connected with the impurity filter on the peristaltic pump, so that external water sequentially passes through the impurity filter, the external input channel, the peristaltic pump, a branch channel, the input channel and the multi-channel rotary valve to enter a filtering channel, and then passes through the filter and the buffer tank on the filtering channel to be diffused, and when the water flows through the filter, the external water is enriched and filtered on the filter;
Step four, reversely opening another peristaltic pump channel connected with the fixing liquid on the peristaltic pump, so that the fixing liquid sequentially passes through the peristaltic pump, the other branch channel, the input channel and the multi-channel rotary valve to enter the same filtering channel, and then passes through the filter and the buffer tank on the filtering channel to be diffused, and when the fixing liquid flows through the filter, the fixing liquid is filtered onto the filter, and the buffer tank realizes micro-storage of the fixing liquid;
Fifthly, the multi-channel rotary valve communicates the rotary valve input port with the next rotary valve output port to realize the filtration of other filtering channels, and the third and fourth steps are repeated;
Step six, sampling and film taking work of the shore base end; recovering to the shore base end, disassembling each filter, taking out the filtering membrane, and completing the whole operation.
The invention relates to another in-situ multichannel water enrichment filtering and fixing method, which adopts the other in-situ multichannel water enrichment filtering and fixing device and comprises the following steps:
Step one, integrally disassembling and cleaning the shore base end; the whole device is disassembled and then cleaned;
Step two, assembling after cleaning, and filling the fixing liquid in an initial state;
Thirdly, lowering the device to the required water depth by utilizing a ship mooring rope, and carrying out enrichment filtration and fixing work; the multi-channel rotary valve is used for communicating a rotary valve input port connected with the enrichment filtering component with a rotary valve output port, starting the peristaltic pump, opening a normally open outlet of a two-position three-way valve, working the enrichment filtering component connected with the rotary valve input port communicated with the rotary valve output port, and enabling external water bodies to sequentially pass through an impurity filter, a filter input channel, a buffer tank, a filter, a tee joint, a filter output channel, a multi-channel rotary valve, an output channel, the peristaltic pump, the normally open outlet of the two-position three-way valve and a water outlet, or/and enabling the external water bodies to be discharged through a third interface of the tee joint after passing through the filter and a drainage pipeline, wherein the external water bodies are enriched and filtered to the upper surface of the filter when flowing through the filter;
The multichannel rotary valve is used for communicating a rotary valve input port connected with fixed liquid with a rotary valve output port, the peristaltic pump is started, a two-position three-way valve normally-closed outlet of the two-position three-way valve is opened, the fixed liquid sequentially passes through the multichannel rotary valve, an output channel, the peristaltic pump, the two-position three-way valve normally-closed outlet, a multichannel connector, a fixed liquid channel, a buffer tank and a filter for enriching external water bodies and is discharged from a three-way third connector through a drainage pipeline, when the fixed liquid flows through the filter for enriching the external water bodies, the fixed liquid is filtered to the upper surface of the filter, and the buffer tank realizes micro storage of the fixed liquid;
fifthly, the multi-channel rotary valve communicates the rotary valve output port with the next rotary valve input port to realize the filtration of other filtering channels, and the third and fourth steps are repeated;
Step six, sampling and film taking work of the shore base end; recovering to the shore base end, disassembling each filter, taking out the filtering membrane, and completing the whole operation.
The invention has the advantages and positive effects that:
1. The invention has the advantages of multiple sampling channels, good fixing effect, flexible and stable work, and can rapidly and effectively acquire the filter membrane sample and perform in-situ fixing.
2. The invention has compact structure and simple design, and the connectivity is finished by using the one-way valve body, thereby reducing a large number of external structures and additional valve bodies.
Drawings
FIG. 1 is a schematic view of a first embodiment of the enrichment filtration and immobilization apparatus of the present invention;
FIG. 2 is a schematic diagram of the multi-channel rotary valve of FIG. 1;
FIG. 3 is a schematic diagram of a second embodiment of the enrichment filtration and immobilization apparatus of the present invention;
Wherein: 1 is a check valve a,2 is a vertical micro water meter, 3 is a buffer tank, 4 is a filter, 5 is a filter channel, 6 is a rotary valve output, 7 is a rotary valve input, 8 is an input channel, 9 is a check valve B,10 is a check valve C,11 is an external input channel, 12 is a peristaltic pump channel a,13 is a peristaltic pump channel B,14 is a peristaltic pump, 15 is a stationary liquid, 16 is a multichannel rotary valve, 17 is a peristaltic pump control cable, 18 is a multichannel rotary valve control cable, 19 is a control and power supply system, 20 is a peristaltic pump channel a outlet, 21 is a peristaltic pump channel a inlet, 22 is a peristaltic pump channel B inlet, 23 is a peristaltic pump channel B outlet, 24 is an impurity filter, 25 is a multichannel rotary valve communication port, 26 is an underwater motor, 27 is an underwater coupler, 28 is a multichannel valve core, 29 is a filter input channel, 30 is a check valve D,31 is a tee, 32 is a filter output channel, 33 is an output channel, 34 is a peristaltic pump inlet, 35 is a peristaltic pump outlet, 36 is a multichannel connector, 37 is a stationary liquid channel, 38 is a two-position three-way valve, 39 is a two-position three-way valve, a two-position normally open outlet, and a three-way valve, a46 is a normally open, and a valve is a valve, and a 42 is a valve.
Detailed Description
The invention is described in further detail below with reference to the accompanying drawings.
Example 1
As shown in fig. 1 and 2, the enrichment filtering and fixing device of the embodiment includes a peristaltic pump 14, a multi-channel rotary valve 16, an enrichment filtering component and a control and power system 19, the peristaltic pump 14 has at least two peristaltic pump channels, wherein the inlet of one peristaltic pump channel is connected with an impurity filter 24 through an external input channel 11, the inlet of the other peristaltic pump channel is connected with a fixing liquid 15, if more peristaltic pump channels are available for communicating with other injectable water bodies, an input channel 8 is arranged between the multi-channel rotary valve 16 and the peristaltic pump 14, one end of the input channel 8 is communicated with a rotary valve input 7 of the multi-channel rotary valve 16, the other end of the input channel 8 is divided into branch channels with the same number as the peristaltic pump channels, and each branch channel is connected with an outlet of one peristaltic pump channel; each branch channel is a parallel channel, each branch channel is provided with a one-way valve which can only feed liquid to the multi-channel rotary valve 16, and the flow of each one-way valve is converted. The embodiment is provided with two peristaltic pump channels, namely a peristaltic pump channel A12 and a peristaltic pump channel B13, wherein an inlet 21 of the peristaltic pump channel A12 is connected with an impurity filter 24 through an external input channel 11, an inlet 22 of the peristaltic pump channel B13 is connected with a fixed liquid 15, and the other end of the input channel 8 is divided into two branch channels which are respectively connected with an outlet 20 of the peristaltic pump channel A12 and an outlet 23 of the peristaltic pump channel B13; the branch channels of the peristaltic pump channel B outlet 23 and the input channel 8 are provided with a one-way valve B9 which can only feed liquid to the multi-channel rotary valve 16, and the branch channels of the peristaltic pump channel A outlet 20 and the input channel 8 are provided with a one-way valve C10 which can only feed liquid to the multi-channel rotary valve 16.
The multi-channel rotary valve 16 is provided with a plurality of rotary valve output ports 6, each rotary valve output port 6 rotates along with the multi-channel rotary valve 16, one end of each rotary valve output port 6 is respectively communicated with the rotary valve input port 7 in the rotation process, the other end of each rotary valve output port 6 is respectively connected with a filtering channel 5, and each filtering channel 5 is provided with an enrichment filtering component.
The enrichment filter assembly of the embodiment comprises a filter channel 5, a filter 4, a buffer tank 3, a vertical micro water meter 2 and a one-way valve A1, wherein one end of the filter channel 5 is connected with a rotary valve output port 6 on a multi-channel rotary valve 16, the other end of the filter channel is connected with a liquid inlet end of the filter 4, a liquid outlet end of the filter 4 is connected with a liquid inlet end of the buffer tank 3, and the liquid outlet end of the buffer tank 3 is sequentially connected with the vertical micro water meter 2 and the one-way valve A1; the buffer tank is used for filtering and buffering the fixing liquid, and then the fixing liquid is diffused through the one-way valve A1, and the fixing liquid in the buffer tank 3 is diffused first, so that the fixing liquid in the filter 4 lasts for a longer time. The vertical micro water meter 2 can measure the water flow filtered by the filter 4 in each filtering channel 5.
The multi-channel valve core 28 of the multi-channel rotary valve 16 of this embodiment is uniformly provided with a plurality of rotary valve output ports 6 along the circumferential direction, the multi-channel rotary valve 16 has a rotary valve input port 7, the multi-channel valve core 28 is connected with the output end of the underwater motor 26 through the underwater coupling 27, the underwater motor 26 drives the multi-channel valve core 28 to rotate, the conversion of each channel on the multi-channel rotary valve 16 is performed, the multi-channel rotary valve communication port 25 of the multi-channel rotary valve 16 is connected with the control and power supply system 19 through the multi-channel rotary valve control cable 18, and the multi-channel rotary valve communication port 25 is the control and power supply port of the underwater motor 26. The peristaltic pump 14 is connected with a control and power supply system 19 through a peristaltic pump control cable 17, and the control and power supply system 19 controls and supplies power to the peristaltic pump 14 and the multi-channel rotary valve 16 respectively to finish forward and reverse conversion of the multi-channel rotary valve 16 and forward and reverse movement of the peristaltic pump 14.
In the embodiment, the opening pressure of each one-way valve is 0.1MPa, and the reverse pressure is 1MPa; all the pipelines of the filtering channel 5 are manufactured by company saint-gobain (holy Gobi) in France; filter 4 is a stainless steel flat filter (142 mm or 90 mm) of milleipore; the fixative is an RNAlater liquid. The control and power system 19 of this embodiment is a prior art and will not be described in detail herein.
The in-situ multichannel water enrichment filtering and fixing method comprises the following steps:
Step one, integrally disassembling and cleaning the shore base end; the whole device is disassembled and then cleaned;
Step two, assembling after cleaning, and filling RNAlater liquid into the fixing liquid 15 in an initial state;
Thirdly, lowering the device to the required water depth by utilizing a ship mooring rope, and carrying out enrichment filtration and fixing work; the control and power supply system 19 controls the multi-channel rotary valve 16 to communicate the rotary valve input port 7 with one rotary valve output port 6, and opens the peristaltic pump channel A12 on the peristaltic pump 14, so that the external water body sequentially passes through the impurity filter 24, the external input channel 11, the peristaltic pump channel A inlet 21, the peristaltic pump channel A outlet 20, the one-way valve C10, the input channel 8, the rotary valve input port 7 and the rotary valve output port 6, then enters one filtering channel 5, and then passes through the filter 4, the buffer tank 3, the vertical micro water meter 2 and the one-way valve A1 on the filtering channel 5 to be diffused, and when the external water body flows through the filter 4, the external water body is enriched and filtered onto the filter 4;
Step four, a peristaltic pump channel B13 connected with the fixed liquid 15 on the peristaltic pump 14 is reversely opened, so that the fixed liquid 15 sequentially passes through an inlet 22 of the peristaltic pump channel B, an outlet 23 of the peristaltic pump channel B, a one-way valve B9, an input channel 8, a rotary valve input port 7 and a rotary valve output port 6 and then enters the same filtering channel 5, and then passes through a filter 4, a buffer tank 3, a vertical micro water meter 2 and a one-way valve A1 on the filtering channel 5 to be diffused, and when the fixed liquid 15 flows through the filter 4, the fixed liquid 15 is filtered onto the filter 4, and the buffer tank 3 realizes micro storage of the fixed liquid 15; that is, the fixing liquid passing through the filter 4 enters the buffer tank 3, and when the fixing liquid is diffused outwards, the fixing liquid in the buffer tank 3 is diffused first, so that the fixing liquid in the filter 4 lasts for a longer time;
Step five, the control and power supply system 19 controls the multi-channel rotary valve 16 to communicate the rotary valve input port 7 with the next rotary valve output port 6, so that the filtration of other filtration channels 5 is realized, and the step three and the step four are repeated;
Step six, sampling and film taking work of the shore base end; recovering to the shore base end, disassembling each filter 4, taking out the filtering membrane, and completing the whole operation.
Example two
As shown in fig. 2 and 3, the enrichment filtering and fixing device of this embodiment includes a peristaltic pump 14, a multi-channel rotary valve 16, a two-position three-way valve 42, an enrichment filtering component, and a control and power system 19, wherein an output channel 33 is disposed between the multi-channel rotary valve 16 and the peristaltic pump 14, one end of the output channel 33 is communicated with a rotary valve output port 6 of the multi-channel rotary valve 16, the other end of the output channel 33 is communicated with a peristaltic pump inlet 34 of the peristaltic pump 14, the multi-channel rotary valve 16 has a plurality of rotary valve input ports 7, each rotary valve input port 7 rotates along with the multi-channel rotary valve 16, one end of each rotary valve input port 7 is respectively communicated with a rotary valve output port 6 during rotation, the other end of one rotary valve input port 7 is connected with a fixing liquid 15, the other ends of the other rotary valve input ports 7 are respectively connected with filtering channels, and each filtering channel is provided with an enrichment filtering component.
The enrichment filtering component of the embodiment comprises an impurity filter 24, a one-way valve A1, a filter input channel 29, a buffer tank 3, a filter 4, a tee joint 31, a vertical micro water meter 2, a filter output channel 32 and a one-way valve D30, wherein one end of the filter output channel 32 is connected with the other end of the rotary valve input port 7, the other end of the filter output channel 32 is connected with one interface of the tee joint 31, a second interface of the tee joint 31 is connected with a liquid outlet end of the filter 4, a liquid inlet end of the filter 4 is connected with a liquid outlet end of the buffer tank 3, the impurity filter 24 is connected with the liquid inlet end of the buffer tank 3 through the filter input channel 29, and the filter input channel 29 is provided with the one-way valve A1 which can only feed liquid into the buffer tank 3; the third joint of the tee joint 31 is connected with a drainage pipeline, and a one-way valve D30 which can only drain water to the outside is arranged on the drainage pipeline.
The peristaltic pump outlet 35 of the peristaltic pump 14 of this embodiment is connected with the two-position three-way valve inlet 39 of the two-position three-way valve 42, the two-position three-way valve normally open outlet 41 of the two-position three-way valve 42 is connected with the water outlet 46 through a pipeline, the two-position three-way valve normally closed outlet 40 of the two-position three-way valve 42 is connected with the liquid inlet end of the multi-channel connector 36, the multi-channel connector 36 has connectors the same number as the enrichment filter components, and each connector is connected to the filter input channel 29 in one enrichment filter component through the fixed liquid channel 37 and is positioned between the one-way valve A1 and the buffer tank 3.
The multi-channel valve core 28 of the multi-channel rotary valve 16 of this embodiment is uniformly provided with a plurality of rotary valve input ports 7 along the circumferential direction, the multi-channel rotary valve 16 has a rotary valve output port 6, the multi-channel valve core 28 is connected with the output end of the underwater motor 26 through the underwater coupling 27, the underwater motor 26 drives the multi-channel valve core 28 to rotate, the conversion of each channel on the multi-channel rotary valve 16 is performed, the multi-channel rotary valve communication port 25 of the multi-channel rotary valve 16 is connected with the control and power supply system 19 through the multi-channel rotary valve control cable 18, and the multi-channel rotary valve communication port 25 is the control and power supply port of the underwater motor 26. Peristaltic pump 14 is connected with control and power supply system 19 through peristaltic pump control cable 17, and two-position three-way valve 42 is connected with control and power supply system 19 through two-position three-way valve control cable 38, and control and power supply system 19 controls and supplies power to peristaltic pump 14, multichannel rotary valve 16 and two-position three-way valve 42 respectively, accomplishes the forward and reverse conversion of multichannel rotary valve 16, the forward and reverse movement of peristaltic pump 14 and the conversion of the normally open outlet and normally closed outlet of two-position three-way valve 42.
In the embodiment, the opening pressure of each one-way valve is 0.1MPa, and the reverse pressure is 1MPa; all the pipelines of the filtering channel 5 are manufactured by company saint-gobain (holy Gobi) in France; filter 4 is a stainless steel flat filter (142 mm or 90 mm) of milleipore; the fixative is an RNAlater liquid. The control and power system 19 of this embodiment is a prior art and will not be described in detail herein.
The in-situ multichannel water enrichment filtering and fixing method comprises the following steps:
Step one, integrally disassembling and cleaning the shore base end; the whole device is disassembled and then cleaned;
Step two, assembling after cleaning, and filling RNAlater liquid into the fixing liquid 15 in an initial state;
thirdly, lowering the device to the required water depth by utilizing a ship mooring rope, and carrying out enrichment filtration and fixing work; the control and power supply system 19 controls the multi-channel rotary valve 16 to communicate a rotary valve input 7 connected with the enrichment filtering component with a rotary valve output 6, and starts the peristaltic pump 14, a two-position three-way valve normally open outlet 41 of a two-position three-way valve 42 is opened, the enrichment filtering component connected with the rotary valve input 7 communicated with the rotary valve output 6 works, external water sequentially passes through the impurity filter 24, the one-way valve A1, the filter input channel 29, the buffer tank 3, the filter 4, the tee joint 31, the filter output channel 32, the rotary valve input 7, the rotary valve output 6, the output channel 33, the peristaltic pump inlet 34, the peristaltic pump outlet 35, a two-position three-way valve inlet 39, a two-position three-way valve normally open outlet 41 and a water outlet 46, or/and external water is discharged from a third interface of the tee joint 31 through a one-way valve D and is enriched and filtered onto the filter 4 when flowing through the filter 4;
Step four, the multi-channel rotary valve 16 communicates the rotary valve input port 7 connected with the fixed liquid 15 with the rotary valve output port 6, the peristaltic pump 14 is started, the two-position three-way valve normally-closed outlet 40 of the two-position three-way valve 42 is opened, the fixed liquid 15 sequentially passes through the rotary valve input port 7, the rotary valve output port 6, the output channel 33, the peristaltic pump inlet 34, the peristaltic pump outlet 35, the two-position three-way valve inlet 39, the two-position three-way valve normally-closed outlet 40, the multi-channel joint 36, the fixed liquid channel 37, the buffer tank 3 and the filter 4 enriching the external water body, and is discharged from the drainage pipeline through the third interface of the three-way valve 31 and the one-way valve D30, when the fixed liquid 15 flows through the filter 4 enriching the external water body, the fixed liquid 15 is filtered onto the filter 4, and the buffer tank 3 realizes the micro-storage of the fixed liquid 15;
Step five, the control and power supply system 19 controls the multi-channel rotary valve 16 to communicate the rotary valve output port 6 with the next rotary valve input port 7, so that the filtration of other filtration channels is realized, and the step three and the step four are repeated;
Step six, sampling and film taking work of the shore base end; recovering to the shore base end, disassembling each filter 4, taking out the filtering membrane, and completing the whole operation.
The invention has compact structure, strong corrosion resistance and multiple enrichment and filtration channels, and can be widely applied to ocean deep sea water body samples needing strict fixation. The invention meets the requirements of high-flux filtration and fixation of deep-sea water, and the acquisition of the original data of DNA and RNA, and can be used for deep-sea microorganism research.
Claims (5)
1. An in-situ multichannel water enrichment filtering and fixing device is characterized in that: the multi-channel rotary valve comprises a peristaltic pump (14), a multi-channel rotary valve (16), a two-position three-way valve (42) and an enrichment filter assembly, wherein an output channel (33) is arranged between the multi-channel rotary valve (16) and the peristaltic pump (14), one end of the output channel (33) is communicated with a rotary valve output port (6) of the multi-channel rotary valve (16), the other end of the output channel (33) is communicated with a peristaltic pump inlet (34) of the peristaltic pump (14), the multi-channel rotary valve (16) is provided with a plurality of rotary valve input ports (7), each rotary valve input port (7) rotates along with the multi-channel rotary valve (16), one end of each rotary valve input port (7) is respectively communicated with the rotary valve output port (6) in the rotation process, the other end of one rotary valve input port (7) is connected with a fixed liquid (15), the other ends of other rotary valve input ports (7) are respectively connected with filter channels, and each filter assembly is arranged on each filter channel; the enrichment filtering component comprises an impurity filter (24), a filter input channel (29), a buffer tank (3), a filter (4), a tee joint (31) and a filter output channel (32), wherein one end of the filter output channel (32) is connected with the other end of the rotary valve input port (7), the other end of the filter output channel (32) is connected with one interface of the tee joint (31), a second interface of the tee joint (31) is sequentially connected with the filter (4) and the buffer tank (3), the impurity filter (24) is connected with the buffer tank (3) through the filter input channel (29), and a third interface of the tee joint (31) is connected with a drainage pipeline; peristaltic pump export (35) of peristaltic pump (14) are connected with two-position three-way valve entry (39) of two-position three-way valve (42), two-position three-way valve normally open export (41) of two-position three-way valve (42) have been connected outlet (46) through the pipeline, two-position three-way valve normally closed export (40) of two-position three-way valve (42) link to each other with multichannel joint (36)'s feed liquor end, multichannel joint (36) have the same joint with enrichment filtration subassembly quantity, and every connects to filter input channel (29) in an enrichment filtration subassembly through stationary liquid passageway (37).
2. The in situ multichannel water body enrichment filtration and fixation device according to claim 1, wherein: the peristaltic pump (14), the multi-channel rotary valve (16) and the two-position three-way valve (42) are respectively connected with the control and power supply system (19), and the control and power supply system (19) respectively controls and supplies power to the peristaltic pump (14), the multi-channel rotary valve (16) and the two-position three-way valve (42) to finish forward and reverse conversion of the multi-channel rotary valve (16), forward and reverse movement of the peristaltic pump (14) and conversion of a normally open outlet and a normally closed outlet of the two-position three-way valve (42).
3. The in situ multichannel water body enrichment filtration and fixation device according to claim 1, wherein: the vertical micro water meter (2) is arranged on a filter output channel (32) between one interface of the tee joint (31) and the rotary valve input port (7), a one-way valve capable of only feeding liquid into the buffer tank (3) is arranged on a filter input channel (29) between the impurity filter (24) and the buffer tank (3), and the fixed liquid channel (37) is connected between the one-way valve and the buffer tank (3).
4. The in situ multichannel water body enrichment filtration and fixation device according to claim 1, wherein: the multi-channel rotary valve is characterized in that a plurality of rotary valve input ports (7) are uniformly arranged on a multi-channel valve core (28) of the multi-channel rotary valve (16) along the circumferential direction, the multi-channel rotary valve (16) is provided with a rotary valve output port (6), the multi-channel valve core (28) is connected with the output end of an underwater motor (26) through an underwater coupler (27), the underwater motor (26) drives the multi-channel valve core (28) to rotate and perform conversion of each channel on the multi-channel rotary valve (16), a multi-channel rotary valve communication port (25) of the multi-channel rotary valve (16) is connected with a control and power supply system (19) through a multi-channel rotary valve control cable (18), and the multi-channel rotary valve communication port (25) is a control and power supply port of the underwater motor (26).
5. An in-situ multichannel water enrichment filtering and fixing method is characterized in that: an in situ multichannel water body enrichment filtration and fixation device as claimed in any of claims 1 to 4, comprising the steps of
Step one, integrally disassembling and cleaning the shore base end; the whole device is disassembled and then cleaned;
Step two, assembling after cleaning, and loading the fixing liquid (15) in an initial state;
Thirdly, lowering the device to the required water depth by utilizing a ship mooring rope, and carrying out enrichment filtration and fixing work; the multi-channel rotary valve (16) is used for communicating a rotary valve input port (7) connected with the enrichment filtering component with a rotary valve output port (6), the peristaltic pump (14) is started, a two-position three-way valve normally-open outlet (41) of the two-position three-way valve (42) is opened, the enrichment filtering component connected with the rotary valve input port (7) communicated with the rotary valve output port (6) works, external water sequentially passes through an impurity filter (24), a filter input channel (29), a buffer tank (3), a filter (4), a tee joint (31), a filter output channel (32), the multi-channel rotary valve (16), an output channel (33), the peristaltic pump (14), the two-position three-way valve normally-open outlet (41) and a water outlet (46), or/and external water is/are discharged through a third interface of a tee joint (31) after passing through the filter (4), and when the external water flows through the filter (4), the external water is filtered onto the filter (4);
Step four, the multi-channel rotary valve (16) is used for communicating a rotary valve input port (7) connected with the fixed liquid (15) with a rotary valve output port (6), the peristaltic pump (14) is started, a two-position three-way valve normally-closed outlet (40) of the two-position three-way valve (42) is opened, the fixed liquid (15) sequentially passes through the multi-channel rotary valve (16), an output channel (33), the peristaltic pump (14), the two-position three-way valve normally-closed outlet (40), a multi-channel connector (36), a fixed liquid channel (37), a buffer tank (3) and a filter (4) enriching external water are discharged through a third interface of a tee joint (31), when the filter (4) enriching external water flows through, the fixed liquid (15) is filtered to the upper surface of the filter (4), and the buffer tank (3) realizes micro storage of the fixed liquid (15);
fifthly, the multi-channel rotary valve (16) communicates the rotary valve output port (6) with the next rotary valve input port (7) to realize the filtration of other filtering channels, and the third and fourth steps are repeated;
Step six, sampling and film taking work of the shore base end; recovering to the shore base end, disassembling each filter (4), taking out the filtering membrane, and completing the whole operation.
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| CN115753310B (en) * | 2022-10-28 | 2023-09-05 | 广东工业大学 | System and method for collecting, filtering, fixing and culturing deep sea pressure-maintaining and heat-preserving microorganisms |
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