CN114354592B - Detection device and detection method for rapid detection of residual pesticide - Google Patents

Abstract

The invention provides a detection device for rapid detection of residual pesticides and a detection method thereof. time, and the solution is stirred by the stirring motor, which can make the solution more evenly stirred, eliminating the need for manual stirring, and improving work efficiency. After the detection solution is configured, there is no need to manually pour the detection solution into the cuvette, and then Put them into the sample detection tank one by one and close the cover. This not only wastes a lot of time, but also cannot ensure that the detection solution is put into the sample detection tank in time, resulting in certain inaccuracies in the detection data, and each time The time required for each detection solution to be put into the sample detection tank is different. When the detection solution is tested on the machine, the detection time for each detection solution is different, which results in inaccurate detection data and affects the experimental results.

Description

A detection device for rapid detection of residual pesticides and its detection method

Technical field

The invention relates to the technical field of agricultural product detection equipment, and in particular to a detection device for rapid detection of residual pesticides and a detection method thereof.

Background technique

Pesticides play a vital role in preventing and controlling pests and diseases, weeding, and regulating crop growth, thereby promoting stable and high yields of crops. Especially in the production process of vegetables, fruits and other agricultural products, organophosphorus and carbamate pesticides are widely used. These two types of pesticides have been used in agriculture for a long time and are highly toxic to humans and animals. With the improvement of people's living standards, pesticide residues in the environment and the toxicity of agricultural products containing pesticide residues have received widespread attention.

Existing rapid detection devices for pesticide residues have certain shortcomings. They require many instruments, are cumbersome in steps, and are inconvenient to use.

Contents of the invention

In view of the above-mentioned deficiencies in the prior art, the present invention provides a detection device for rapid detection of residual pesticides and a detection method thereof. for

To achieve the above-mentioned object of the invention, the technical solutions adopted by the present invention are:

A detection device for rapid detection of residual pesticides, including: a housing, a support frame is provided on the upper side of the housing, a reaction box is provided on the support frame, and a stirring motor is provided at the bottom of the reaction box , a stirring rod is provided inside the reaction box, and the stirring rod is connected to the rotating shaft of the stirring motor. A filter screen is provided inside the reaction box, and the filter screen is located above the stirring rod. A solenoid valve is provided on one side of the bottom of the reaction box, a connecting pipe is provided below the solenoid valve, a control panel is provided on the upper side of the housing away from the support frame, and a display screen is provided on the control panel. , a detection placement slot is provided above the housing, the detection placement slot is located between the support frame and the control panel, a sample detection slot is provided at the bottom of the detection placement slot, and the detection placement slot is A cover plate is provided on one side, and a drip nozzle is provided on the cover plate. One end of the drip nozzle is connected to an end of the communication tube away from the reaction box. An electronic scale is provided above the housing. A laser is provided inside the housing, a photoelectric receiving tube is provided inside the housing, a power transmission module is provided on one side of the interior of the housing, and a side of the housing away from the power transmission module A main control module is provided on one side of the housing, a signal conditioning module is provided on one side of the housing close to the main control module, and a support base is provided below the housing.

As an improvement, a cuvette is provided inside the sample detection tank.

As an improvement, the laser and the photoelectric receiving tube are arranged on both sides of the sample detection slot correspondingly.

As an improvement, after the cover is closed, the drip nozzle is located directly above the sample detection slot.

As an improvement, the display screen is a touch screen.

As an improvement, the drip tip and the cover plate can be detached.

As an improvement, the communication pipe is a hose.

As an improvement, the reaction box is made of glass material, and the reaction box and the support frame are detachable.

A detection method using a detection device for rapid detection of residual pesticides is characterized in that it includes the following steps:

Step 1. Plug in the power and turn on a detection device for rapid detection of residual pesticides. A screen will appear on the display. Follow the prompts on the screen to start self-test, which will take about 1 minute. After the self-test is completed, it enters the standby test state.

Step 2. Prepare detection reagents:

1. Buffer: Add 1 packet of buffer to 500 mL of distilled or purified water, stir and dissolve to make a phosphate buffer, pH 7.6, and store at room temperature.

2. Chromogenic reagent: Take 1 bottle of chromogenic reagent and add 25mL of buffer to dissolve it. Take 100μL before use and store it in a refrigerator at 4°C.

3. Substrate: Take 1 bottle of substrate and add 12.5 mL of distilled or purified water to dissolve it. When using, take 100 μL and store it in a refrigerator at 4°C; or take the bottom of 1 bottle

Add 2.5 mL of distilled or purified water to dissolve the solution. Take 20 μL before use and store in a 4°C refrigerator.

4. Cholinesterase: The enzyme preparation does not need to be prepared and can be used directly. When using, take 100 μL and store it in the refrigerator at 4°C.

Note: Reagent manufacturers are different, please refer to the instructions when preparing.

Step 3: Take 2g of fruit and vegetable samples (4g for tubers), cut leafy vegetables into about 25px square pieces, take cross-section samples or skin of tubers, put them into a conical flask, add 10mL buffer, and shake for 1 to 2 minutes , pour out the extraction liquid, let it stand for 2 minutes, and wait for testing. If the extraction solution is turbid or has too many impurities, it can be filtered and then tested again.

Step 3: First perform a control test. Add 2.5 mL of buffer solution to the reaction bottle, then add 100 μL of enzyme solution and chromogenic reagent, mix well, let stand for 10 minutes, add 100 μL or 20 μL of substrate, shake well, and immediately pour into the mixture. In the color dish, put it into the sample detection tank in time, close the cover, adjust the test interface for the required detection parameters through the display screen on the control panel, touch the control button on the display screen, a quick test method for residual pesticides The detection device of the test starts to perform the comparison. After 3 minutes, the comparison is completed. The control sample is taken out and the interface of the display remains unchanged. Then perform sample testing. First, put the cuvette into the sample testing tank, close the cover, put 2.5ml of the extraction solution into the reaction box, then add 100μL of enzyme solution and chromogenic reagent respectively, and mix through the motor. After allowing the reaction to stand for 10 minutes, add 100 μL or 20 μL of substrate, mix well, and immediately open the solenoid valve. The solution will enter the cuvette through the connecting tube, the drip nozzle, and the test will be carried out through the touch screen. After the test is completed, the screen will display Absorbance increment ΔΑt) and inhibition rate. Data is saved automatically.

The beneficial effects of the present invention are:

The detection device for rapid detection of residual pesticides is equipped with an electronic scale to facilitate sample measurement, and also effectively prevents the loss of the electronic scale. The detection device for rapid detection of residual pesticides is provided with multiple reaction boxes, and the reaction boxes are There is a stirring motor at the bottom, multiple reaction boxes can be tested at the same time, saving a lot of time, and the solution is stirred by the stirring motor, which can make the solution stir more evenly, eliminating manual stirring, improving work efficiency, and detecting solution configuration After completion, there is no need to manually pour the detection solution into the cuvette, and then put it into the sample detection tank one by one, and then close the cover. This not only wastes a lot of time, but also cannot ensure that the detection solution is timely. Put it into the sample detection tank, resulting in certain inaccuracies in the detection data, and the time when each detection solution is put into the sample detection tank is different. When the detection solution is tested on the machine, the detection time of each detection solution is different, so This leads to inaccurate detection data and affects the experimental results.

Description of the drawings

Figure 1 is a schematic structural diagram of the present invention;

Figure 2 is a schematic diagram of the internal structure of the present invention;

Figure 3 is an enlarged structural view of point A in Figure 1.

Reference symbol comparison table:

1. Shell; 2. Support frame; 3. Reaction box; 4. Stirring rod; 5. Filter; 6. Stirring motor; 7. Solenoid valve; 8. Connecting tube; 9. Cover plate; 10. Drip tip; 11. Control panel; 12. Display screen; 13. Sample detection tank; 14. Electronic scale; 15. Laser; 16. Photoelectric receiving tube; 17. Power transmission module; 18. Signal conditioning module; 19. Main control module; 20. Support base.

Detailed ways

In order to make the content of the present invention easier to understand clearly, the technical solutions in the embodiments of the present invention will be clearly and completely described below in conjunction with the drawings in the embodiments of the present invention. Identical components are designated with the same reference numerals. It should be noted that the words "front", "back", "left", "right", "upper" and "lower" used in the following description refer to the directions in the drawings, and the words "inside" and "outside" ” refers to the direction toward or away from the geometric center of a specific part, respectively.

Example 1

As shown in Figures 1 and 2, a detection device for rapid detection of residual pesticides includes: a housing 1. A support frame 2 is provided on the upper side of the housing 1. A reaction box 3 is provided on the support frame 2. A stirring motor 6 is provided at the bottom of the reaction box 3. There is a stirring motor 6 inside the reaction box 3. Stirring rod 4, wherein the stirring rod 4 is connected to the rotating shaft of the stirring motor 6, a filter 5 is provided inside the reaction box 3, the filter 5 is located above the stirring rod 4, the reaction box 3 A solenoid valve 7 is provided on one side of the bottom of the box 3, and a communication pipe 8 is provided below the solenoid valve 7. The communication pipe 8 is a hose, and the upper side of the housing 1 is away from the support frame 2. A control panel 11 is provided. A display screen 12 is provided on the control panel 11. A detection and placement slot 21 is provided above the housing 1. The detection and placement slot 21 is located between the support frame 2 and the control panel 11. In between, a sample detection tank 13 is provided at the bottom of the detection placement tank 21, wherein a cuvette is provided inside the sample detection tank 13, and a cover 9 is provided on one side of the detection placement tank 21. The cover The plate 9 is provided with a drip nozzle 10. One end of the drip nozzle 10 is connected to an end of the connecting pipe 8 away from the reaction box 3. An electronic scale 14 is provided above the housing 1. The housing 1 A laser 15 is provided inside, a photoelectric receiving tube 16 is provided inside the housing 1, a power transmission module 17 is provided on one side of the housing 1, and the inside of the housing 1 is far away from the power transformer. A main control module 19 is provided on one side of the transmission module 17 , a signal conditioning module 18 is provided on a side of the housing 1 close to the main control module 19 , and a support base 20 is provided below the housing 1 .

Referring to Figure 2, the laser 15 and the photoelectric receiving tube 16 are correspondingly arranged on both sides of the sample detection tank 13. After the cover 9 is closed, the drip nozzle 10 is located at the side of the sample detection tank 13. Directly above.

It should be noted that the display screen 12 is a touch screen, which facilitates touch control through the display screen 12, realizes human-machine interface interaction, and improves the overall practicality. The drip nozzle 10 and the cover plate 9 can be detached. After the sample detection is completed, the drip nozzle 10 can be detached from the cover plate 9 to facilitate cleaning and replacement of the drip nozzle 10 .

The reaction box 3 is made of glass material. The reaction box 3 and the support frame 2 can be disassembled. After the sample detection is completed, the reaction box 3 needs to be removed from the support frame 2 and the reaction box 3 needs to be cleaned. , to prevent mutual contamination of samples caused by the next test.

Example 2

A detection method using a detection device for rapid detection of residual pesticides is characterized in that it includes the following steps:

Step 1. Plug in the power and turn on a detection device for rapid detection of residual pesticides. A screen will appear on the display. Follow the prompts on the screen to start self-test, which will take about 1 minute. After the self-test is completed, it enters the standby test state.

Step 2. Prepare detection reagents:

1. Buffer: Add 1 packet of buffer to 500 mL of distilled or purified water, stir and dissolve to make a phosphate buffer with pH 7.6, and store at room temperature.

2. Chromogenic reagent: Take 1 bottle of chromogenic reagent and add 25mL of buffer to dissolve it. Take 100μL before use and store it in a refrigerator at 4°C.

3. Substrate: Take 1 bottle of substrate and add 12.5 mL of distilled or purified water to dissolve it. When using, take 100 μL and store it in a refrigerator at 4°C; or take 1 bottle of substrate and add 2.5 mL of distilled or purified water to dissolve it. When using, take 20 μL and store 4 ℃ refrigerator.

4. Cholinesterase: The enzyme preparation does not need to be prepared and can be used directly. When using, take 100 μL and store it in the refrigerator at 4°C.

Note: Reagent manufacturers are different, please refer to the instructions when preparing.

Step 3: Take 2g apple sample, cut the apple into small pieces, put it into an Erlenmeyer flask, add 10mL buffer, shake for 1 to 2 minutes, pour out the extract, and let it stand for 2 minutes before testing. If the extraction solution is turbid or has too many impurities, it can be filtered and then tested again.

Step 4: First perform a control test. Add 2.5 mL of buffer solution to the reaction bottle, then add 100 μL of enzyme solution and chromogenic reagent, mix well, let stand for 10 minutes, add 100 μL or 20 μL of substrate, shake well, and immediately pour into the mixture. In the color dish, put it into the sample detection tank in time, close the cover, adjust the test interface for the required detection parameters through the display screen on the control panel, touch the control button on the display screen, a quick test method for residual pesticides The detection device of the test starts to perform the comparison. After 3 minutes, the comparison is completed. The control sample is taken out and the interface of the display remains unchanged. Then perform sample testing. First, put the cuvette into the sample testing tank, close the cover, put 2.5ml of the extraction solution into the reaction box, then add 100μL of enzyme solution and chromogenic reagent respectively, and mix through the motor. After allowing the reaction to stand for 10 minutes, add 100 μL or 20 μL of substrate, mix well, and immediately open the solenoid valve. The solution will enter the cuvette through the connecting tube, the drip nozzle, and the test will be carried out through the touch screen. After the test is completed, the screen will display Absorbance increment ΔΑt) and inhibition rate. Data is saved automatically.

Example 3

A detection method using a detection device for rapid detection of residual pesticides is characterized in that it includes the following steps:

Step 1. Plug in the power and turn on a detection device for rapid detection of residual pesticides. A screen will appear on the display. Follow the prompts on the screen to start self-test, which will take about 1 minute. After the self-test is completed, it enters the standby test state.

Step 2. Prepare detection reagents:

1. Buffer: Take 1 packet of buffer and add 500mL of distilled or purified water, stir and dissolve to make phosphate buffer.

pH7.6, store at room temperature.

2. Chromogenic reagent: Take 1 bottle of chromogenic reagent and add 25mL of buffer to dissolve it. Take 100μL before use and store it in a refrigerator at 4°C.

3. Substrate: Take 1 bottle of substrate and add 12.5 mL of distilled or purified water to dissolve it. When using, take 100 μL and store it in a refrigerator at 4°C; or take 1 bottle of substrate and add 2.5 mL of distilled or purified water to dissolve it. When using, take 20 μL and store 4 ℃ refrigerator.

4. Cholinesterase: The enzyme preparation does not need to be prepared and can be used directly. When using, take 100 μL and store it in the refrigerator at 4°C.

Note: Reagent manufacturers are different, please refer to the instructions when preparing.

Step 3: Take 2g banana sample, cut the banana into small pieces, put it into an Erlenmeyer flask, add 10mL buffer solution, shake for 1 to 2 minutes, pour out and extract.

liquid, let it sit for 2 minutes, and wait for testing. If the extraction solution is turbid or has too many impurities, it can be filtered and then tested again.

Step 4: First perform a control test. Add 2.5 mL of buffer solution to the reaction bottle, then add 100 μL of enzyme solution and chromogenic reagent, mix well, let stand for 10 minutes, add 100 μL or 20 μL of substrate, shake well, and immediately pour into the mixture. In the color dish, put it into the sample detection tank in time, close the cover, adjust the test interface for the required detection parameters through the display screen on the control panel, touch the control button on the display screen, a quick test method for residual pesticides The detection device of the test starts to perform the comparison. After 3 minutes, the comparison is completed. The control sample is taken out and the interface of the display remains unchanged. Then perform sample testing. First, put the cuvette into the sample testing tank, close the cover, put 2.5ml of the extraction solution into the reaction box, then add 100μL of enzyme solution and chromogenic reagent respectively, and mix through the motor. After allowing the reaction to stand for 10 minutes, add 100 μL or 20 μL of substrate, mix well, and immediately open the solenoid valve. The solution will enter the cuvette through the connecting tube, the drip nozzle, and the test will be carried out through the touch screen. After the test is completed, the screen will display Absorbance increment ΔΑt) and inhibition rate. Data is saved automatically.

Example 4

A detection method using a detection device for rapid detection of residual pesticides is characterized in that it includes the following steps:

Step 1. Plug in the power and turn on a detection device for rapid detection of residual pesticides. A screen will appear on the display. Follow the prompts on the screen to start self-test, which will take about 1 minute. After the self-test is completed, it enters the standby test state.

Step 2. Prepare detection reagents:

1. Buffer: Add 1 packet of buffer to 500 mL of distilled or purified water, stir and dissolve to make a phosphate buffer with pH 7.6, and store at room temperature.

2. Chromogenic reagent: Take 1 bottle of chromogenic reagent and add 25mL of buffer to dissolve it. Take 100μL before use and store it in a refrigerator at 4°C.

3. Substrate: Take 1 bottle of substrate and add 12.5 mL of distilled or purified water to dissolve it. When using, take 100 μL and store it in a refrigerator at 4°C; or take 1 bottle of substrate and add 2.5 mL of distilled or purified water to dissolve it. When using, take 20 μL and store 4 ℃ refrigerator.

4. Cholinesterase: The enzyme preparation does not need to be prepared and can be used directly. When using, take 100 μL and store it in the refrigerator at 4°C.

Note: Reagent manufacturers are different, please refer to the instructions when preparing.

Step 3: Take 2g of lettuce sample, cut the leafy vegetable into about 25px square pieces, put it into an Erlenmeyer flask, add 10mL of buffer solution, shake for 1 to 2 minutes, pour out the extract, and let it stand for 2 minutes before testing. If the extraction solution is turbid or has too many impurities, it can be filtered and then tested again.

Step 4: First perform a control test. Add 2.5 mL of buffer solution to the reaction bottle, then add 100 μL of enzyme solution and chromogenic reagent, mix well, let stand for 10 minutes, add 100 μL or 20 μL of substrate, shake well, and immediately pour into the mixture. In the color dish, put it into the sample detection tank in time, close the cover, adjust the test interface for the required detection parameters through the display screen on the control panel, touch the control button on the display screen, a quick test method for residual pesticides The detection device of the test starts to perform the comparison. After 3 minutes, the comparison is completed. The control sample is taken out and the interface of the display remains unchanged. Then perform sample testing. First, put the cuvette into the sample testing tank, close the cover, put 2.5ml of the extraction solution into the reaction box, then add 100μL of enzyme solution and chromogenic reagent respectively, and mix through the motor. After allowing the reaction to stand for 10 minutes, add 100 μL or 20 μL of substrate, mix well, and immediately open the solenoid valve. The solution will enter the cuvette through the connecting tube, the drip nozzle, and the test will be carried out through the touch screen. After the test is completed, the screen will display Absorbance increment (ΔΑt) and inhibition rate. Data is saved automatically.

The above are only preferred embodiments of the present invention patent and are not intended to limit the present invention patent. Any modifications, equivalent substitutions and improvements made within the spirit and principles of the present invention patent shall be included in Within the protection scope of the patent of this invention.

Claims (9)

1. A detection device for rapid detection of residual pesticides, characterized in that it includes: a housing (1), a support frame (2) is provided on the upper side of the housing (1), and the support frame (2) 2) is provided with a reaction box (3), a stirring motor (6) is provided at the bottom of the reaction box (3), a stirring rod (4) is provided inside the reaction box (3), and the stirring rod (4) 4) Connected to the rotating shaft of the stirring motor (6), a filter (5) is provided inside the reaction box (3), and the filter (5) is located above the stirring rod (4), so A solenoid valve (7) is provided on one side of the bottom of the reaction box (3), a connecting pipe (8) is provided below the solenoid valve (7), and the upper part of the housing (1) is away from the support frame ( 2) is provided with a control panel (11) on one side, a display screen (12) is provided on the control panel (11), and a detection placement slot (21) is provided above the housing (1). The placement slot (21) is located between the support frame (2) and the control panel (11). A sample detection slot (13) is provided at the bottom of the detection placement slot (21). The detection placement slot (21) ) is provided with a cover plate (9) on one side, and a drip nozzle (10) is provided on the cover plate (9). One end of the drip nozzle (10) and the communication tube (8) are away from the reaction box (3) is connected to one end of the housing (1), an electronic scale (14) is provided above the housing (1), a laser (15) is provided inside the housing (1), and a laser (15) is provided inside the housing (1). There is a photoelectric receiving tube (16), and a power transmission module (17) is provided on one side of the housing (1). A side of the housing (1) away from the power transmission module (17) A main control module (19) is provided on the side of the housing (1). A signal conditioning module (18) is provided on the side of the housing (1) close to the main control module (19). A support is provided below the housing (1). Seat(20). 2. A detection device for rapid detection of residual pesticides according to claim 1, characterized in that a cuvette is provided inside the sample detection tank (13). 3. A detection device for rapid detection of residual pesticides according to claim 2, characterized in that the laser (15) and the photoelectric receiving tube (16) are correspondingly arranged in the sample detection tank ( 13) on both sides. 4. A detection device for rapid detection of residual pesticides according to claim 1, characterized in that after the cover (9) is closed, the drip nozzle (10) is located in the sample detection tank (13) ) directly above. 5. A detection device for rapid detection of residual pesticides according to claim 1, characterized in that the display screen (12) is a touch screen. 6. A detection device for rapid detection of residual pesticides according to claim 1, characterized in that the drip nozzle (10) and the cover plate (9) are detachable. 7. A detection device for rapid detection of residual pesticides according to claim 1, characterized in that the connecting pipe (8) is a hose. 8. A detection device for rapid detection of residual pesticides according to claim 1, characterized in that the reaction box (3) is made of glass material, and the reaction box (3) and the support frame (2) Can be disassembled. 9. A detection method using a detection device for rapid detection of residual pesticides according to any one of claims 1 to 8, characterized in that it includes the following steps: Step 1. Plug in the power and turn on a detection device for rapid detection of residual pesticides. A screen will appear on the display. Follow the prompts on the screen to start self-test. It will take about 1 minute. After the self-test is completed, it will enter the standby detection state; Step 2. Prepare detection reagents: 1. Buffer: Add 1 packet of buffer to 500 mL of distilled or purified water, stir and dissolve to make a phosphate buffer with a pH of 7.6, and store it at room temperature; 2. Chromogen: Take 1 bottle of chromogen and add 25mL of buffer to dissolve it. Take 100μL before use and store it in the refrigerator at 4°C; 3. Substrate: Take 1 bottle of substrate and add 12.5 mL of distilled or purified water to dissolve it. When using, take 100 μL and store it in a refrigerator at 4°C; or take 1 bottle of substrate and add 2.5 mL of distilled or purified water to dissolve it. When using, take 20 μL and store 4 ℃ refrigerator storage; 4. Cholinesterase: The enzyme preparation does not need to be prepared and can be used directly. When using, take 100 μL and store it in a refrigerator at 4°C; Step 3: Take 2g of fruit and vegetable samples, 4g of tubers, cut leafy vegetables into about 25px square pieces, take cross-section samples of tubers or their skin, put them into a conical flask, add 10mL buffer, and shake for 1 to 2 minutes. Pour out the extraction solution and let it sit for 2 minutes before testing. If the extraction solution is turbid or has too many impurities, filter it and then test again; Step 4: First perform a control test. Add 2.5 mL of buffer solution to the reaction bottle, then add 100 μL of enzyme solution and chromogenic reagent, mix well, let stand for 10 minutes, add 100 μL or 20 μL of substrate, shake well, and immediately pour into the mixture. In the color dish, put it into the sample detection tank in time, close the cover, adjust the test interface for the required detection parameters through the display screen on the control panel, touch the control button on the display screen, a quick test method for residual pesticides The detection device starts to perform comparison. After 3 minutes, the comparison is completed. Take out the control sample, keep the display interface unchanged, and then perform sample detection. First, put the cuvette into the sample detection tank, close the cover, and Put 2.5ml of the extraction solution into the reaction box, then add 100μL of enzyme solution and chromogenic reagent respectively, mix through the motor, let stand for 10 minutes, add 100μL or 20μL of substrate, mix and immediately open the solenoid valve, the solution will pass through The connecting tube enters the cuvette through the drip tip, and is detected through the touch screen. After the detection is completed, the screen displays the absorbance increment ΔΑt and the inhibition rate, and the data is automatically saved.