CN114354554B - Preparation method and application of detection platform for full-time line biomarker - Google Patents

Abstract

The invention relates to the field of material preparation, detection and analysis, in particular to a preparation method and application of a detection platform for full-time line biomarkers, wherein the method comprises the following steps: preparing a super-hydrophilic-hydrophobic glass surface patterning template; preparing an oil-in-water emulsion; and synthesizing a surface patterning oil-water gel droplet array to obtain the detection platform for the full-time line biomarker. The beneficial effects of the invention are as follows: the detection platform takes the surface patterning oil-water gel as a substrate, has simple preparation process and good stability, and can prolong the application time of the substrate to 120min compared with the traditional detection method. Based on the principle that the surface patterning oil hydrogel controls water evaporation and water is supplied upwards, long-time moisture preservation of liquid drops can be realized.

Description

Preparation method and application of a detection platform for full-timeline biomarkers

Technical field

The invention is in the field of material preparation and detection analysis, and relates to a preparation method and application of a detection platform for full-timeline biomarkers.

Background technique

Accurate and sensitive identification of biomarkers is essential in various fields from prognosis, disease diagnosis to basic biological sciences. However, traditional non-absorbent chips face deficiencies in droplet hydration, resulting in incomplete reactions and detection interruptions. However, existing moisturizing strategies are not universal and will have an impact on the detection process. Therefore, it is of great significance to prepare a graphics chip with good water retention performance.

Plant leaves include a waxy coating and stomata that establish a homeostatic structure that retains water. The stomata of plants serve as communication channels between plants and the volatile external environment and play an important role in the effective use of water. The wax layer can prevent excessive water loss caused by excessive evaporation of water. Here, inspired by the water-retaining structure of leaves, we report a biosensor based on surface-patterned oil hydrogel materials with good water-retaining capabilities and can be used for full-timeline biosensing.

Contents of the invention

The invention discloses a preparation method and application of a detection platform for full-timeline biomarkers to solve any of the above and other potential problems of the prior art.

In order to solve the above problems, the technical solution of the present invention is: a preparation method for a detection platform for full-timeline biomarkers, which method specifically includes the following steps:

S1) Preparation of super hydrophilic-hydrophobic glass surface patterned template;

S2) prepare oil-in-water emulsion;

S3) Solidify the oil-in-water emulsion obtained in S2) on the surface patterned template obtained in S1) to synthesize a surface patterned oil hydrogel droplet array, thereby obtaining a detection platform for full-timeline biomarkers.

Further, the specific steps of S1) are:

S1.1) Select a template, and after pretreatment, modify the template surface to obtain a template with surface self-assembly into a hydrophobic layer.

S1.2) Then clamp the mask plate to S1.1) through the pattern mold to obtain the template surface, place it in a plasma cleaning machine and process it for 3-8 minutes to obtain a super hydrophilic-hydrophobic glass surface patterned template.

Further, the modification process in S1.1) is: in a vacuum environment, the temperature is 55-65°C, the modification time is 5.5-6.5 hours, the modification product: 1H, 1H, 2H, 2H-perfluorodecyltrimethyl Oxysilane.

Further, the specific process of S2) is:

S2.1) Prepare hydrogel prepolymer; weigh deionized water, hydroxyethyl acrylate, nanoclay and 2,2-diethoxyacetophenone, mix and stir for 1-1.5h to obtain oil gel Glue prepolymer;

S2.2) Prepare the oil gel prepolymer: weigh lauryl methacrylate, stearyl methacrylate, ethylene glycol dimethacrylate and 2,2-diethoxyacetophenone respectively. Mix and stir for 1-1.5h to obtain the oil gel prepolymer;

S2.3) Mix the oleogel prepolymer obtained in S2.2) and the hydrogel prepolymer obtained in S2.1) according to the mass ratio of 1:1.24, add it to the cell crusher, and process 1 at a power of 580W -3min to form an oil-in-water emulsion with oil droplets having a particle size of 2-10 μm.

Further, in S2.1): the mass percentage of each component of the hydrogel prepolymer is: deionized water: 81wt%-86wt%, hydroxyethyl acrylate: 10wt%-15wt%, nanoclay: 3wt %-4wt%,, 2,2-diethoxyacetophenone: 0.03wt%-0.05wt%.

Further, the mass percentage of each component of the oil gel prepolymer in S2.2) is: lauryl methacrylate: 45wt%-50wt%, stearyl methacrylate: 45wt%-50wt%, Ethylene glycol methacrylate: mass fraction 3wt%-4wt%, 2,2-diethoxyacetophenone: 0.7wt%-1wt%.

Further, the specific steps of S3) are:

S3.1) Pour the oil-in-water emulsion obtained in S2) into the super hydrophilic-hydrophobic glass surface patterned template obtained in S1);

S3.2) Irradiate under ultraviolet light with a wavelength of 365nm and a light power of 12mW cm ^-2 for 12-18 minutes to obtain a polymerized gel.

S3.3) Soak the gel in water to remove unreacted monomers and impurities, and fully swell to obtain a detection platform for full-timeline biomarkers.

A detection platform for full-timeline biomarkers prepared by the above method can be applied to the field of biomarker detection in various time periods.

A detection platform for full-timeline biomarkers, which is prepared by the above-mentioned preparation method.

The present invention also provides a detection method using the above-mentioned full-timeline biomarker detection platform. The detection method specifically includes the following:

First, mix the test solution and the mixed solution into 1×PBS buffer to obtain reaction droplets containing a certain concentration.

Add the reaction solution to the hydrophilic area of OH-gel, add water to the humidity of 20-60, record the fluorescence signal measurement every 30 minutes under a laser confocal scanning microscope, and place the droplets in the hydrogel area for reaction . Take photos and records every 20 minutes to obtain the relationship between the time and signal of the biomarker detection.

The beneficial effects of the present invention are: due to the adoption of the above technical solution, the surface patterned oil hydrogel obtained through the wetting transfer strategy of the present invention has accurately controllable hydrophobic oil gel areas and hydrophilic hydrogel areas. The oil gel area on the surface of the template is hydrophobic, which can reduce the evaporation of water inside the gel. The hydrogel area is hydrophilic, and water evaporates from the porous hydrogel area. This characteristic of directional evaporation of water can provide a high-humidity environment for droplets fixed in the hydrophilic area. At the same time, the supporting structure of the gel is an internal uniform oil-hydrogel structure, and the continuous hydrogel phase is a water-filled three-dimensional network structure that provides a source of moisture for moisturizing. The dispersed oleogel phase and the continuous hydrogel phase jointly form capillary channels, and capillary force promotes the upward transport of water to the surface hydrogel area to meet the long-term detection needs. This moisturizing strategy can be used for reaction droplets. Create a high-humidity environment to achieve long-term hydration without affecting biological reactions. Long-term hydration can be achieved through the self-water supply inside the gel and its ability to be connected to external water sources. As an open droplet reaction platform, this biosensing array can be applied to biomarker detection in a variety of time periods and combined with a variety of detection methods. Compared with traditional detection methods, the substrate application time can be extended to 120 minutes. .

Picture description:

Figure 1 is a schematic diagram of the surface patterned oil hydrogel of the present invention.

Figure 2 is a diagram showing the relationship between time and signal of biomarker detection provided by the present invention compared with the ordinary super infiltration chip (SHI/SHO).

Detailed ways:

The technical solution of the present invention will be further described below in conjunction with the accompanying drawings and specific embodiments.

As shown in Figure 1, the present invention is a method for preparing a detection platform for full-timeline biomarkers. The method specifically includes the following steps:

S1) Preparation of super hydrophilic-hydrophobic glass surface patterned template;

S2) prepare oil-in-water emulsion;

S3) Synthesize surface-patterned oil-hydrogel droplet arrays to obtain a detection platform for full-timeline biomarkers. The platform has the surface partitioning structure of hydrogels and oleogels, and also has a uniform support structure of oil-hydrogels. .

The specific steps of S1) are:

S1.1) Select a template, and after pretreatment, modify the template surface to obtain a template with surface self-assembly into a hydrophobic layer.

S1.2) Then clamp the mask plate to S1) through the pattern mold to obtain the template surface, place it in a plasma cleaning machine and process it for 3-8 minutes to obtain a super hydrophilic-hydrophobic glass surface patterned template.

The modification process in S1.1) is: in a vacuum environment, the temperature is 55-65°C, the modification time is 5.5-6.5 hours, the modification product: 1H, 1H, 2H, 2H-perfluorodecyltrimethoxy Silane.

The specific process of S2) is:

S2.1) Prepare hydrogel prepolymer; weigh deionized water, hydroxyethyl acrylate, nanoclay and 2,2-diethoxyacetophenone, mix and stir for 1-1.5h to obtain oil gel Glue prepolymer;

S2.2) Prepare the oil gel prepolymer: weigh lauryl methacrylate, stearyl methacrylate, ethylene glycol dimethacrylate and 2,2-diethoxyacetophenone respectively. Mix and stir for 1-1.5h to obtain the oil gel prepolymer;

S2.3) Mix the oleogel prepolymer obtained in S2.2) and the hydrogel prepolymer obtained in S2.1) according to the mass ratio of 1:1.24, add it to the cell crusher, and process 1 at a power of 580W -3min to form an oil-in-water emulsion with a particle size of 2-10 μm.

In said S2.1), the mass percentage of each component of the hydrogel prepolymer is: deionized water: 81wt%-86wt%, hydroxyethyl acrylate: 10wt%-15wt%, nanoclay: 3wt%- 4wt%, 2,2-diethoxyacetophenone: 0.03wt%-0.05wt%.

Further, the mass percentage of each component of the oil gel prepolymer in S2.2) is: lauryl methacrylate 45wt%-50wt%, stearyl methacrylate: 45wt%-50wt%, Ethylene glycol dimethacrylate: mass fraction 3wt%-4wt%, 2,2-diethoxyacetophenone: 0.7wt%-1wt%.

The nanoclay brand Laponite XLS, composition [Mg _5.34 Li _0.66 Si ₈ O ₂₀ (OH) ₄ ]Na _0.66 , 20-30nm diameter and 1nm thickness, MW=762.24.).

The specific steps of S3) are:

S3.1) Pour the oil-in-water emulsion obtained in S2) into the super hydrophilic-hydrophobic glass surface patterned template obtained in S1);

S3.2) Irradiate under ultraviolet light with a wavelength of 365nm and a light power of 12mW cm ^-2 for 12-18 minutes to obtain a gel that has completed polymerization.

S3.3) Soak the gel in water to remove unreacted monomers and impurities, and fully swell to obtain a detection platform for full-timeline biomarkers;

Surface patterned oleohydrogel has precisely controllable hydrophobic oleogel regions and hydrophilic hydrogel regions. The oil gel area on the surface of the template is hydrophobic, which can reduce the evaporation of water inside the gel. The hydrogel area is hydrophilic, and water evaporates from the porous hydrogel area. This characteristic of directional evaporation of water can provide a high-humidity environment for droplets fixed in the hydrophilic area. At the same time, the supporting structure of the gel is an internal uniform oil-hydrogel structure, and the continuous hydrogel phase is a water-filled three-dimensional network structure that provides a source of moisture for moisturizing. The dispersed oleogel phase and the continuous hydrogel phase jointly form capillary channels, and capillary force promotes the upward transport of water to the surface hydrogel area to meet the long-term detection needs. This moisturizing strategy can be used for reaction droplets. Create a high-humidity environment to achieve long-term hydration without affecting biological reactions. Long-term hydration can be achieved through the self-water supply inside the gel and its ability to be connected to external water sources.

A detection platform for full-timeline biomarkers prepared by the above method can be applied to the field of biomarker detection in various time periods.

Example 1

Select a 10cm*10cm glass as a template, clean its surface, and modify it in a vacuum environment of 1H,1H,2H,2H-perfluorodecyltrimethoxysilane at 60°C for 6h to make 1H,1H,2H,2H -Perfluorodecyltrimethoxysilane self-assembles into a hydrophobic layer on the glass surface, and then clamps the mask with 2mm diameter micropores on the glass surface through a dovetail clamp, places it in a plasma environment for 5 minutes, and then takes out the glass slices, and a super hydrophilic-hydrophobic glass surface patterned template was obtained. Configure the oil hydrogel prepolymer. The components of the hydrogel prepolymer are: 60g water, 8.55g hydroxyethyl acrylate, 2.4g nanoclay, and 30mg 2,2-diethoxyacetophenone. Stir for 4 hours. Prepare the oil gel prepolymer: 38.1g lauryl methacrylate, 38.1g stearyl methacrylate, 3g ethylene glycol dimethacrylate, 60mg 2,2-diethoxyacetophenone, stir 1h. The oleogel hydrogel was mixed at a mass ratio of 1:1.24 and processed under a cell disruptor for 1 min. Forms a uniform oil-in-water emulsion. Prepare the patterned glass surface obtained in step (1) into a grooved mold, pour the oil-hydrogel prepolymer into the mold, and irradiate it under 365nm UV for 15 minutes. Remove the polymerized gel. Soak the gel in water to remove unreacted monomers and impurities, and swell fully.

Configure Fe ³⁺ reaction droplets: Add 10 nM FeCl ₃ solution dropwise into 60 mM potassium thiocyanide solution. And place 10 μl droplets on the hydrogel area for reaction. Take photos every 1 minute and record the results (as shown in Figure 2).

Example 2

Select a 10cm×10cm glass as a template, clean its surface, and modify it in a vacuum environment of 1H,1H,2H,2H-perfluorodecyltrimethoxysilane at 60°C for 6h to make 1H,1H,2H,2H -Perfluorodecyltrimethoxysilane self-assembles into a hydrophobic layer on the glass surface, and then clamps the mask with 2mm diameter micropores on the glass surface through a dovetail clamp, places it in a plasma environment for 5 minutes, and then takes out the glass slices, and a super hydrophilic-hydrophobic glass surface patterned template was obtained. Configure the oil hydrogel prepolymer. The components of the hydrogel prepolymer are: 60g water, 8.55g hydroxyethyl acrylate, 2.4g nanoclay, and 30mg 2,2-diethoxyacetophenone. Stir for 4 hours. Prepare the oil gel prepolymer: 38.1g lauryl methacrylate, 38.1g stearyl methacrylate, 3g ethylene glycol dimethacrylate, 60mg 2,2-diethoxyacetophenone, stir 1h. The oleogel hydrogel was mixed at a mass ratio of 1:1.24 and processed under a cell disruptor for 1 min. Forms a uniform oil-in-water emulsion. Prepare the patterned glass surface obtained in step (1) into a grooved mold, pour the oil-hydrogel prepolymer into the mold, and irradiate it under 365nm UV for 15 minutes. Remove the polymerized gel. Soak the gel in water to remove unreacted monomers and impurities, and swell fully.

Configure the reaction droplets of vitamin B: add 10mM vitamin B solution dropwise into the 60mM diazotized p-aminobenzene sulfonic acid solution. And place 10 μl droplets on the hydrogel area for reaction. Take photos and record the results every 3 minutes (as shown in Figure 2).

Example 3

Select a 10cm×10cm glass as a template, clean its surface, and modify it in a vacuum environment of 1H,1H,2H,2H-perfluorodecyltrimethoxysilane at 60°C for 6h to make 1H,1H,2H,2H -Perfluorodecyltrimethoxysilane self-assembles into a hydrophobic layer on the glass surface, and then clamps the mask with 2mm diameter micropores on the glass surface through a dovetail clamp, places it in a plasma environment for 5 minutes, and then takes out the glass slices, and a super hydrophilic-hydrophobic glass surface patterned template was obtained. Configure oil hydrogel prepolymer. The hydrogel prepolymer composition list is: 60g water, 8.55g hydroxyethyl acrylate, 2.4g nanoclay, 30mg 2,2-diethoxyacetophenone and stir evenly for 4 hours. Configure oil gel prepolymer: 38.1g lauryl methacrylate, 38.1g stearyl methacrylate, 3g ethylene glycol dimethacrylate, 60mg 2,2-diethoxyacetophenone uniformly Stir for 1h. The oleogel hydrogel was mixed at a mass ratio of 1:1.24 and processed under a cell disruptor for 1 min. Forms a uniform oil-in-water emulsion. Prepare the patterned glass surface obtained in step (1) into a grooved mold, pour the oil-hydrogel prepolymer into the mold, and irradiate it under 365nm UV for 15 minutes. Remove the polymerized gel. Soak the gel in water to remove unreacted monomers and impurities, and swell fully.

Configure vitamin C reaction droplets: add 10mM vitamin C solution dropwise into 60mM ammonium molybdate aqueous solution. And place 10 μl droplets on the hydrogel area for reaction. Take photos every 5 minutes and record the results (as shown in Figure 2).

Example 4

Select a 10cm×10cm glass as a template, clean its surface, and modify it in a vacuum environment of 1H,1H,2H,2H-perfluorodecyltrimethoxysilane at 60°C for 6h to make 1H,1H,2H,2H -Perfluorodecyltrimethoxysilane self-assembles into a hydrophobic layer on the glass surface, and then clamps the mask with 2mm diameter micropores on the glass surface through a dovetail clamp, places it in a plasma environment for 5 minutes, and then takes out the glass slices, and a super hydrophilic-hydrophobic glass surface patterned template was obtained. Configure oil hydrogel prepolymer. The hydrogel prepolymer composition list is: 60g water, 8.55g hydroxyethyl acrylate, 2.4g nanoclay, 30mg 2,2-diethoxyacetophenone and stir evenly for 4 hours. Configure oil gel prepolymer: 38.1g lauryl methacrylate, 38.1g stearyl methacrylate, 3g ethylene glycol dimethacrylate, 60mg 2,2-diethoxyacetophenone uniformly Stir for 1h. The oleogel hydrogel was mixed at a mass ratio of 1:1.24 and processed under a cell disruptor for 1 min. Forms a uniform oil-in-water emulsion. Prepare the patterned glass surface obtained in step (1) into a grooved mold, pour the oil-hydrogel prepolymer into the mold, and irradiate it under 365nm UV for 15 minutes. Remove the polymerized gel. Soak the gel in water to remove unreacted monomers and impurities, and swell fully.

Configure the reaction droplets of PDGF-BB: When detecting PDGF-BB, mix 50nM PDGF-BB and 200nM H1, DNA1, and DNA2 solutions into 1×PBS buffer to a final volume of 0.1mL. Add 10 ul of the reaction solution to the hydrophilic area of OH-gel and react at 25°C and 50% humidity. Record fluorescence signal measurements every 30 minutes under a confocal scanning microscope. And place the droplets in the hydrogel area for reaction. Take photos every 20 minutes and record the results (as shown in Figure 2). The nucleic acid sequences used are as follows, and they were all synthesized by Sangon Bioengineering Co., Ltd. (Beijing).

Example 5

Select a 10cm×10cm glass as a template, clean its surface, and modify it in a vacuum environment of 1H,1H,2H,2H-perfluorodecyltrimethoxysilane at 60°C for 6h to make 1H,1H,2H,2H -Perfluorodecyltrimethoxysilane self-assembles into a hydrophobic layer on the glass surface, and then clamps the mask with 2mm diameter micropores on the glass surface through a dovetail clamp, places it in a plasma environment for 5 minutes, and then takes out the glass slices, and a super hydrophilic-hydrophobic glass surface patterned template was obtained. Configure oil hydrogel prepolymer. The hydrogel prepolymer composition list is: 60g water, 8.55g hydroxyethyl acrylate, 2.4g nanoclay, 30mg 2,2-diethoxyacetophenone and stir evenly for 4 hours. Configure oil gel prepolymer: 38.1g lauryl methacrylate, 38.1g stearyl methacrylate, 3g ethylene glycol dimethacrylate, 60mg 2,2-diethoxyacetophenone uniformly Stir for 1h. The oleogel hydrogel was mixed at a mass ratio of 1:1.24 and processed under a cell disruptor for 1 min. Forms a uniform oil-in-water emulsion. Prepare the patterned glass surface obtained in step (1) into a grooved mold, pour the oil-hydrogel prepolymer into the mold, and irradiate it under 365nm UV for 15 minutes. Remove the polymerized gel. Soak the gel in water to remove unreacted monomers and impurities, and swell fully.

Configure RABV reaction droplets: In 1×TBS buffer, mix 100 nM RABV and 400 nM HP-A, HP-B, and HP-C solutions to a final volume of 0.1 mL. Add 10ul of the reaction solution to the hydrophilic area of OH-gel, continue to add water, and react at 25°C and 50% humidity. Fluorescence signal measurements were recorded under a laser confocal scanning microscope at intervals of 30 minutes, and the results were shown in Figure 2. The nucleic acid sequences used are as follows, and they were all synthesized by Sangon Bioengineering Co., Ltd. (Beijing).

The above is a detailed introduction to the preparation method and application of a detection platform for full-timeline biomarkers provided in the embodiments of the present application. The description of the above embodiments is only used to help understand the method and the core idea of the present application; at the same time, for those of ordinary skill in the art, there will be changes in the specific implementation and application scope based on the ideas of the present application. In summary, the contents of this specification should not be construed as limiting this application.

For example, certain words are used in the description and claims to refer to specific components. Those skilled in the art will understand that hardware manufacturers may use different names to refer to the same component. This specification and the claims do not use differences in names as a means to distinguish components; rather, differences in functions of the components serve as a criterion for distinction. For example, the words "include" and "include" mentioned in the entire description and claims are open-ended terms, so they should be interpreted as "includes/includes but is not limited to." "Approximately" means that within an acceptable error range, those skilled in the art can solve the technical problem within a certain error range and basically achieve the technical effect. The following descriptions of the specification are preferred implementation modes for implementing the present application. However, the descriptions are for the purpose of illustrating the general principles of the present application and are not intended to limit the scope of the present application. The scope of protection of this application shall be determined by the appended claims.

It should also be noted that the terms "includes", "includes" or any other variation thereof are intended to cover a non-exclusive inclusion, such that a good or system that includes a list of elements includes not only those elements but also those not expressly listed other elements, or elements inherent to the product or system. Without further limitation, an element defined by the statement "comprises a..." does not exclude the presence of other identical elements in the goods or systems that include the stated element.

It should be understood that the term "and/or" used in this article is only an association relationship describing related objects, indicating that there can be three relationships, for example, A and/or B, which can mean: A alone exists, and A and A exist simultaneously. B, there are three situations of B alone. In addition, the character "/" in this article generally indicates that the related objects are an "or" relationship.

The above description shows and describes several preferred embodiments of the present application, but as mentioned above, it should be understood that the present application is not limited to the form disclosed herein, and should not be regarded as excluding other embodiments, but can be used in various Other combinations, modifications and environments, and can be modified through the above teachings or technology or knowledge in related fields within the scope of the application concept described herein. Any modifications and changes made by those skilled in the art that do not deviate from the spirit and scope of this application shall be within the protection scope of the appended claims of this application.

Claims (5)

1. A method for preparing a detection platform for full-timeline biomarkers, characterized in that the method specifically includes the following steps: S1) Preparation of superhydrophilic-hydrophobic surface patterned templates; The specific steps are: S1.1) Select a template, and after pretreatment, modify the surface of the template to obtain a template with a surface that self-assembles into a hydrophobic layer. The modification process is: in a vacuum environment, the temperature is 55-65°C, and the modification time is 5.5-6.5 Hours, modification: 1H,1H,2H,2H-perfluorodecyltrimethoxysilane; S1.2) Then clamp the mask plate to S1.1) through the pattern mold to obtain the template surface, place it in a plasma cleaning machine and process it for 3-8 minutes to obtain a super hydrophilic-hydrophobic glass surface patterned template; S2) prepare oil-in-water emulsion; The specific process is: S2.1) Prepare the hydrogel prepolymer; take deionized water, hydroxyethyl acrylate, nanoclay and 2,2-diethoxyacetophenone, mix and stir for 1-1.5h to obtain the oil gel prepolymer. liquid gathering; S2.2) Prepare the oil gel prepolymer: weigh lauryl methacrylate, stearyl methacrylate, ethylene glycol dimethacrylate and 2,2-diethoxyacetophenone respectively. Mix and stir for 1-1.5h to obtain the oil gel prepolymer; S2.3) Mix the oleogel prepolymer obtained in S2.2) and the hydrogel prepolymer obtained in S2.1) according to the mass ratio of 1:1.24, add it to the cell crusher, and process 1 at a power of 580W -3min to form an oil-in-water emulsion with a particle size of 2-10 μm; S3) Solidify the oil-in-water emulsion obtained in S2) on the surface patterned template obtained in S1) to synthesize a surface patterned oil hydrogel droplet array, thereby obtaining a detection platform for full-timeline biomarkers; The specific steps are: S3.1) Pour the oil-in-water emulsion obtained in S2) onto the super hydrophilic-hydrophobic surface patterned template obtained in S1); S3.2) Irradiate under ultraviolet light with a wavelength of 365nm and a light power of 12mW cm ^-2 for 12-18 minutes to obtain a gel that has completed polymerization. S3.3) Soak the gel in water to remove unreacted monomers and impurities, and fully swell to obtain a detection platform for full-timeline biomarkers. 2. The preparation method according to claim 1, characterized in that the mass percentage of each component of the hydrogel prepolymer in S2.1) is: deionized water: 81wt%-86wt%, acrylic acid hydroxyl Ethyl ester: 10wt%-15wt%, nanoclay: 3wt%-4wt%, 2,2-diethoxyacetophenone: 0.03wt%-0.05wt%. 3. The preparation method according to claim 1, characterized in that the mass percentage of each component of the oil gel prepolymer in S2.2) is: lauryl methacrylate 45wt%-50wt%, methyl Octadecyl acrylate: 45wt%-50wt%, ethylene glycol dimethacrylate: 3wt%-4wt%, 2,2-diethoxyacetophenone: 0.7wt%-1wt%. 4. A detection platform for full-timeline biomarkers prepared by the method according to any one of claims 1-3 and applied to the field of biomarker detection in various time periods. 5. A detection platform for full-timeline biomarkers, characterized in that the detection platform for full-timeline biomarkers is prepared by the preparation method described in any one of claims 1-3.