CN114324262B - A fluorescence detection optical path system and spectroscopic structure - Google Patents
A fluorescence detection optical path system and spectroscopic structure Download PDFInfo
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- CN114324262B CN114324262B CN202011047168.XA CN202011047168A CN114324262B CN 114324262 B CN114324262 B CN 114324262B CN 202011047168 A CN202011047168 A CN 202011047168A CN 114324262 B CN114324262 B CN 114324262B
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- 230000003287 optical effect Effects 0.000 title claims abstract description 36
- 238000001917 fluorescence detection Methods 0.000 title claims abstract description 30
- 230000005284 excitation Effects 0.000 claims abstract description 29
- 239000003153 chemical reaction reagent Substances 0.000 claims abstract description 25
- 238000001514 detection method Methods 0.000 abstract description 15
- 238000010586 diagram Methods 0.000 description 2
- 238000000034 method Methods 0.000 description 2
- 230000000149 penetrating effect Effects 0.000 description 2
- 230000007547 defect Effects 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 238000001914 filtration Methods 0.000 description 1
- 230000004907 flux Effects 0.000 description 1
- 239000011521 glass Substances 0.000 description 1
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- 238000003753 real-time PCR Methods 0.000 description 1
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Abstract
本发明公开了一种该荧光检测光路系统及分光结构，该荧光检测光路系统包括光源、准直镜片、光源滤光片、棱镜片组、二向色镜、荧光相机。其中，准直镜片设于光源的后级光路中；光源滤光片设于准直镜片的后级光路中；棱镜片组设于光源滤光片的后级光路中；二向色镜设于棱镜片组的后级光路中；荧光相机设于样本试剂的上方。本发明的分光结构包括多个棱镜片组。本发明的荧光检测光路系统能够提高样本试剂的检测效率，相应的，该分光结构能够将激发光分成多束光强相等的光束，满足大通量样本试剂的检测需求。
The present invention discloses a fluorescence detection optical path system and a spectroscopic structure, wherein the fluorescence detection optical path system includes a light source, a collimating lens, a light source filter, a prism lens group, a dichroic mirror, and a fluorescence camera. The collimating lens is arranged in the post-stage optical path of the light source; the light source filter is arranged in the post-stage optical path of the collimating lens; the prism lens group is arranged in the post-stage optical path of the light source filter; the dichroic mirror is arranged in the post-stage optical path of the prism lens group; and the fluorescence camera is arranged above the sample reagent. The spectroscopic structure of the present invention includes a plurality of prism lens groups. The fluorescence detection optical path system of the present invention can improve the detection efficiency of the sample reagent, and accordingly, the spectroscopic structure can divide the excitation light into a plurality of light beams with equal light intensity, so as to meet the detection requirements of large-throughput sample reagents.
Description
Technical Field
The invention relates to the technical field of detection, in particular to a fluorescence detection light path system and a light splitting structure.
Background
In a conventional fluorescence detection device, a detection head is usually used for corresponding to one sample reagent, and then scanning is performed to detect all sample reagents. The detection principle is that in the fluorescence detection process, excitation light emitted by a light source is collimated and filtered, and then irradiates the sample reagent through a dichroic mirror, so that the sample reagent is excited to generate fluorescence, and a fluorescence signal is captured by a fluorescence camera, so that the detection of the sample reagent is realized.
In the current environment, as the population of the world increases, the detection demand is increasing, and the conventional fluorescence detection device (for example, fluorescence quantitative PCR) can only detect one sample reagent at a time, which results in a decrease in detection efficiency. Therefore, the conventional fluorescence detection device has a low detection speed for a large flux of sample reagent.
Disclosure of Invention
In order to solve the defects in the prior art, the invention aims to provide a fluorescence detection light path system and a light splitting structure, wherein the fluorescence detection light path system can improve the detection efficiency of a sample reagent, and correspondingly, the light splitting structure can split excitation light into a plurality of light beams with equal light intensity, so that the detection requirement of a large-flux sample reagent is met.
The technical scheme adopted by the invention for solving the technical problems is that the fluorescent detection light path system comprises:
A light source;
The collimating lens is arranged in a rear-stage light path of the light source and is used for collimating light beams emitted by the light source;
The light source filter is arranged in a rear-stage light path of the collimating lens and used for filtering the collimated light beam to form excitation light;
The prism sheet group is arranged in a rear-stage light path of the light source filter and used for splitting the excitation light so that the excitation light is divided into a plurality of light beams with equal light intensity through the prism sheet group;
a dichroic mirror provided in a rear-stage optical path of the prism sheet group so that the split excitation light is directed to the sample reagent;
The fluorescence camera is arranged above the sample reagent, so that fluorescence generated by excitation of the sample reagent by the excited light is shot by the fluorescence camera;
The prism sheet group comprises a plurality of prism sheets, one side surface of each prism sheet is a light receiving surface, the other side surface of each prism sheet is a light splitting surface, and the light receiving surfaces of the prism sheets are perpendicular to the optical axis of the light beam.
Based on the technical scheme, when the sample reagent is detected, the light beams emitted by the light source are collimated by the collimating lens and filtered by the light source filter to form excitation light, the excitation light is split by the prism lens group, so that the excitation light is split into a plurality of light beams with equal light intensity, and the plurality of light beams can respectively detect different sample reagents, thereby meeting the detection requirement on the large-flux sample reagent and improving the detection efficiency. Meanwhile, the prism sheet adopted by the invention is provided with the light receiving surface and the light splitting surface, and when the prism sheet is used, the light receiving surface and the optical axis of the light beam are vertically distributed, so that the light beam can be totally reflected in the prism sheet, and the light intensity loss of the light beam is reduced.
The present invention may also have the following additional technical features according to the fluorescence detection optical path system of the present invention.
Further, according to the fluorescence detection light path system of the present invention, the light receiving surface of the prism sheet is a plane, and the light splitting surface of the prism sheet has regular triangle convex strips distributed in an array, and by the arrangement of the plane and the regular triangle convex strips, the light beam can be split into two light beams with equal light intensity when penetrating the prism sheet.
Further, according to the fluorescence detection optical path system of the present invention, a mirror is further provided in a front-stage optical path of the dichroic mirror, and the light beam having passed through the prism sheet component light is reflected to a mirror surface of the dichroic mirror by the mirror.
Further, according to the fluorescence detection light path system of the present invention, the prism sheet group includes three prism sheets, and three prism sheets are arranged in a regular triangle shape therebetween;
In the three prism sheets, the light-splitting surface of one prism sheet faces the light-receiving surfaces of the other two prism sheets.
Further, according to the fluorescence detection light path system of the present invention, the prism sheet group includes six prism sheets, wherein three prism sheets constitute a first light-splitting assembly, and the other three prism sheets constitute a second light-splitting assembly provided in a rear light path of the first light-splitting assembly.
Further, according to the fluorescence detection light path system of the present invention, in the second light splitting assembly, two prism sheets have the same length, and the length of the remaining prism sheet is adapted to the length of the coverage surface of the light beam split by the first light splitting assembly, so that the light beam split by the first light splitting assembly is totally reflected to the second light splitting assembly.
Based on the technical scheme of the fluorescence detection light path system, the invention also provides a light splitting structure, which comprises:
a prism sheet set provided with at least one set;
The prism sheet group comprises a plurality of prism sheets, one side surface of each prism sheet is a light receiving surface, the other side surface of each prism sheet is a light splitting surface, and the light receiving surfaces of the prism sheets are perpendicular to the optical axis of the light beam.
Optionally, the prism sheet group includes three prism sheets.
Optionally, the light receiving surface of the prism sheet is a plane, and the light splitting surface of the prism sheet has regular triangle protrusions distributed in an array.
Drawings
Fig. 1 is a schematic view of the structure of a prism sheet of the present invention;
fig. 2 is a side view of the prism sheet of the present invention;
FIG. 3 is a spectroscopic schematic of the prism sheet of the present invention;
fig. 4 is a schematic structural view of a prism sheet set of embodiment 1 of the present invention;
fig. 5 is a schematic structural view of a second spectroscopic assembly of embodiment 2 of the present invention;
FIG. 6 is a schematic diagram of an optical path system according to embodiment 1 of the present invention;
fig. 7 is a schematic diagram of an optical path system according to embodiment 2 of the present invention.
Detailed Description
The application is described in further detail below with reference to the drawings and examples. It is to be understood that the specific embodiments described herein are merely illustrative of the application and are not limiting of the application. It should be noted that, for convenience of description, only the portions related to the application are shown in the drawings.
It should be noted that, without conflict, the embodiments of the present application and features of the embodiments may be combined with each other. The application will be described in detail below with reference to the drawings in connection with embodiments.
In one aspect of the present invention, a fluorescence detection light path system is provided. The fluorescence detection light path system comprises a light source, a collimating lens, a light source filter 200, a prism sheet set, a dichroic mirror 300 and a fluorescence camera 400. The light source filter 200 is arranged in a rear-stage light path of the light source and filters the collimated light beams to form excitation light, the prism sheet group is arranged in the rear-stage light path of the light source filter and splits the excitation light into a plurality of light beams with equal light intensity, the dichroic mirror 300 is arranged in the rear-stage light path of the prism sheet group and guides the split excitation light to the sample reagent, and the fluorescent camera 400 is arranged above the sample reagent and enables fluorescence generated by excitation light excitation of the sample reagent 500 to be shot through the fluorescent camera 400.
Fig. 1 is a schematic structural view of a prism sheet of the present invention, and fig. 2 is a side view of the prism sheet of the present invention.
Referring to fig. 1 and 2, in the fluorescence detection light path system of the present invention, a prism sheet set includes a plurality of prism sheets 100, one side surface of the prism sheet 100 is a light receiving surface, and the other side surface is a light splitting surface. In the fluorescence detection light path system of the present invention, the light receiving surface of the prism sheet 100 is perpendicular to the optical axis of the light beam, so that the incident light can be ensured to enter the prism sheet 100 perpendicularly.
As shown in fig. 2, the light receiving surface of the prism sheet 100 is a plane 101, while the light splitting surface of the prism sheet 100 has regular triangle-shaped protrusions 102 distributed in an array, the protrusions 102 are distributed along the width direction of the prism sheet 100, and the root portions of every two adjacent protrusions 102 are connected together, so that no gap exists between the root portions of the protrusions 102.
In the fluorescence detection optical path system of the present invention, since the convex strips 102 of the prism sheet 100 are regular triangles, the top corners of the convex strips 102 are 60 °, in which case, when the light beam enters the prism sheet 100 from the light receiving surface, the light beam is directed in the direction of the convex strips 102 after completely penetrating the plane 101. Referring to fig. 3, since the angle between the edge of the ridge 102 and the plane of the prism sheet 100 is 60 °, the light beam is totally reflected after encountering the edge of the ridge 102, and is reflected toward the other edge of the ridge 102, and the reflected light beam is perpendicular to the other edge of the ridge 102, so that the light beam directly passes through the other edge of the ridge 102 completely. Since the convex bar 102 has two edges, the light beam passing through the prism sheet 100 is divided into two equal light beams by the convex bar 102, and the included angle between the two light beams is 120 °.
The prism sheet 100 of the present invention may be made of optical plastic or glass, which is molded by a molding process.
In the fluorescence detection optical path system of the present invention, a mirror 600 is further provided in the front-stage optical path of the dichroic mirror 300, and the light beam having passed through the prism sheet component light is reflected to the mirror surface of the dichroic mirror 300 by the mirror 600.
Based on the fluorescence detection light path system, the invention also provides a light splitting structure which can split a light beam into a plurality of light beams with equal light intensity.
The spectroscopic structure of the present invention includes:
The prism sheet group is provided with at least one group;
The prism sheet set includes a plurality of prism sheets 100, one side surface of the prism sheet 100 is a light receiving surface, the other side surface is a light splitting surface, and the light receiving surface of the prism sheet 100 is perpendicular to the optical axis of the light beam. Referring to fig. 1 and 2, the light receiving surface of the prism sheet 100 is a plane 101, and the light splitting surface of the prism sheet 100 has regular triangle convex strips 102 distributed in an array.
The invention will now be described with reference to specific examples, which are intended to be illustrative only and not limiting in any way.
Example 1
In the present embodiment, the excitation light is set to be divided into 4 beams. Therefore, in the present embodiment, the prism sheet group includes three prism sheets 100, the specifications of the three prism sheets 100 are identical, and the three prism sheets 100 are connected end to end, so that the three prism sheets 100 are arranged in a regular triangle shape, and the arrangement of the three prism sheets refers to the arrangement mode of fig. 4.
In the three prism sheets 100, the light-splitting surface of one prism sheet 100 faces the light-receiving surfaces of the other two prism sheets, that is, the light-splitting surface of one prism sheet 100 is in contact with the light-receiving surfaces of the other two prism sheets 100, and the light-receiving surfaces of the one prism sheet 100 and the light-splitting surfaces of the other two prism sheets 100 are both disposed outward.
According to the arrangement of the prism sheets 100 of the present embodiment, as shown in fig. 6, after the excitation light passes through the prism sheet 100 with the light receiving surface facing outward, the excitation light is split into two light beams with an included angle of 120 °, the two light beams are respectively directed to the other two prism sheets 100, the two light beams respectively pass through the other two prism sheets 100 and are split into two light beams again, and the total light intensity of the 4 light beams is uniform. Since one of the two light beams finally split is parallel to the excitation light, the light beam is directed to the dichroic mirror 300, and the other light beam has an included angle of 120 ° with the excitation light, so that the light beam is reflected by the mirror 600 to be parallel to the excitation light, and is directed to the dichroic mirror 300. Since the mirror surface of the dichroic mirror 300 is 45 ° distributed to the excitation light, the 4 light beams are directed to different positions of the dichroic mirror 300, and the dichroic mirror 300 reflects the 4 light beams to 4 different sample reagents for detection.
Example 2
In the present embodiment, the excitation light is set to be divided into 16 beams. Therefore, this embodiment may further add a set of prism sheet groups on the basis of embodiment 1, that is, in this embodiment, the prism sheet groups used include six prism sheets 100, where three prism sheets 100 form a first light splitting assembly, that is, the prism sheet group in embodiment 1, and the other three prism sheets form a second light splitting assembly, and the second light splitting assembly is disposed in a subsequent optical path of the first light splitting assembly.
In this embodiment, in the second beam splitter, as shown in fig. 5, the lengths of two prism sheets 100 are identical, and the lengths of the two prism sheets 100 are also identical to those of the prism sheet 100 in the first beam splitter, and the length of the remaining prism sheet is adapted to the length of the coverage of the beam split by the first beam splitter, so that the beam split by the first beam splitter is totally reflected to the second beam splitter.
As shown in fig. 7, the 4 light beams split by the first beam splitter are split by the second beam splitter, respectively, so that the light beams are split into 16 light beams with equal light intensity.
In this embodiment, the light splitting principles of the first light splitting component and the second light splitting component are identical to those of the prism sheet set in embodiment 1, and are not described herein.
The above description is only illustrative of the preferred embodiments of the present application and of the principles of the technology employed. It will be appreciated by persons skilled in the art that the scope of the application referred to in the present application is not limited to the specific combinations of the technical features described above, but also covers other technical features formed by any combination of the technical features described above or their equivalents without departing from the inventive concept. Such as the above-mentioned features and the technical features disclosed in the present application (but not limited to) having similar functions are replaced with each other.
Other technical features besides those described in the specification are known to those skilled in the art, and are not described herein in detail to highlight the innovative features of the present invention.
Claims (5)
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| CN202011047168.XA CN114324262B (en) | 2020-09-27 | 2020-09-27 | A fluorescence detection optical path system and spectroscopic structure |
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| CN202011047168.XA CN114324262B (en) | 2020-09-27 | 2020-09-27 | A fluorescence detection optical path system and spectroscopic structure |
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| US7387891B2 (en) * | 1999-05-17 | 2008-06-17 | Applera Corporation | Optical instrument including excitation source |
| JP6377887B2 (en) * | 2012-02-17 | 2018-08-22 | 学校法人慶應義塾 | Liquid crystal display |
| CN104297910A (en) * | 2014-09-03 | 2015-01-21 | 长春理工大学 | Multi-wavelength composite beam splitting and receiving device |
| CN211554567U (en) * | 2019-12-30 | 2020-09-22 | 昆山龙腾光电股份有限公司 | Prism lens and backlight module with same |
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