CN114252324B - An easy-to-use porous brain slice incubation device - Google Patents

Abstract

The invention relates to a conveniently-used porous brain slice incubation device, which comprises an incubation device body; an incubation chamber and an oxygen passage are arranged in the incubation device body, the oxygen passage is communicated with the incubation chamber, and the oxygen passage is connected with an oxygen supply pipeline; the incubation chamber is internally provided with an incubation plate which is movably arranged inside the incubation chamber; the incubation plate is provided with at least one incubation hole, and an incubation net for bearing brain slices/tissue slices is arranged below the incubation hole; the incubation chamber is internally provided with a separator which divides the incubation chamber into different spaces; a temperature control device is arranged outside the incubation device body, and the temperature control device still uses the principle of a water bath method; the temperature can be well kept by incubation under the water bath condition, and the temperature is controlled and heated uniformly; placing the brain slice/tissue slice in the incubation hole in such a way, providing oxygen for the brain slice/tissue slice by using the oxygen supply pipeline and the oxygen passage, and incubating; the isolation piece is arranged, so that the waste of incubation liquid during incubation of a single brain slice can be effectively avoided.

Description

An easy-to-use porous brain slice incubation device

technical field

The invention belongs to the technical field of medical devices, and in particular relates to a convenient-to-use porous brain slice incubation device.

Background technique

Brain slice is a commonly used specimen in neuroscience research. The method is to take the brain tissue or spinal cord completely out of the animal body and cut it into slices of 200-500 μm in artificial cerebrospinal fluid. These thin slices retain the activity and function of part of the neural tissue, and have the advantage of retaining relatively complete neural circuits and extracellular environment compared with discrete or cultured cells; compared with animals in vivo, the influencing factors are relatively simple and more predictable. In the preparation of brain slices, multiple brain slices are usually cut for the brain region of interest, and then the brain slices are placed in artificial cerebrospinal fluid for incubation. The common method is to place all brain slices in one incubation bucket. Incubate. However, the overall shape of each brain region is irregular, and there will be transition changes in different directions. Therefore, distinguishing which segment of the whole brain region the excised brain slice is located in in the experiment can help to understand the functional and morphological differences of different segments of the brain region, which is of great significance to the overall understanding of the brain region of interest. It is more sensitive to the surrounding culture environment, and a larger disturbance will affect the activity of the brain slices. Keeping the relative independence of the incubation environment of each brain slice helps to reduce the disturbance caused by selecting one brain slice to other brain slices.

At present, the commonly used brain slice incubation buckets are mostly placed together to incubate all the cut brain slices. This incubation method has the following defects: 1. All brain slices are mixed together. When one of the brain slices is selected, it is easy to disturb other brain slices next to it. On the one hand, this disturbance will disrupt the placement of the original brain slices. On the other hand, this disturbance will stimulate the neurons and glial cells in the brain slice, causing unnecessary activation of neurons, affecting the neuronal Activity and function; 2. At present, the incubation temperature of brain slices is mostly carried out under two conditions of 34 degrees and room temperature, but at present, the temperature is mostly maintained under water bath conditions. When the temperature is controlled by the water bath method, once the water level is lower than the incubator, the incubator will tilt, which will affect the normal incubation of the brain slices; and if a new incubation solution is added, the temperature will fluctuate, affecting the brain slices. / The activity of the tissue slices will affect the experimental results; 3. The existing brain slice incubation structures are all devices used for the incubation of multiple brain slices. When multiple brain slices are placed together, they are easily stacked and affect the activity; 4. When incubating a single brain slice/tissue slice in the incubator, the incubation solution is wasted.

Aiming at the above problems, the present invention provides a convenient-to-use porous brain slice incubation device, which effectively ensures a suitable incubation environment, and also solves the problem that multiple incubations can be incubated together, but a single incubation can effectively save incubation liquid.

SUMMARY OF THE INVENTION

In order to overcome the problems raised in the background art, the present invention provides a convenient-to-use porous brain slice incubation device.

An easy-to-use porous brain slice incubation device, comprising an incubation device body; an incubation chamber and an oxygen passageway are arranged in the incubation device body, the oxygen passageway is communicated with the incubation chamber, and the oxygen passageway is connected to an oxygen supply pipeline; the incubation chamber An incubation plate is arranged inside, and the incubation plate is movable inside the incubation chamber; the incubation plate is provided with at least one incubation hole, and an incubation net for carrying brain slices/tissue slices is arranged below the incubation hole; an isolation piece is arranged in the incubation chamber, The separator divides the interior of the incubation chamber into different spaces; a temperature control device is arranged outside the incubation device body, and the temperature control device still uses the principle of the water bath method; because the incubation under the condition of a water bath can maintain the temperature well, and control the temperature It is heated evenly to ensure a suitable temperature environment; in this way, the brain slice/tissue slice is placed in the incubation hole, and the oxygen supply pipeline and oxygen passage are used to supply oxygen to the brain slice/tissue slice and incubate; Effectively avoid wasting the incubation solution when incubating a single brain slice.

Further, a cover plate is arranged on the upper end of the incubation device body, and the cover plate covers the incubation device body to ensure the sealing of the incubation chamber and the oxygen passage.

Further, the first end of the oxygen passage is arranged at the lower end of the incubation chamber, the second end of the oxygen passage is arranged at the left end or the right end of the incubation chamber, and the oxygen passage and the incubation chamber form a closed-loop mouth shape; this setting can drive the incubation Fluid flows, but the oxygen circuit is not on the incubation plate, preventing blow-off force on brain/tissue pieces.

Further, the incubation net is made of nylon silk to ensure normal incubation of the brain slices/tissue slices; the incubation plate is made of acrylic.

Further, a stop bar is arranged on the inner side wall of the incubation chamber, and the incubation plate is placed on the upper end of the stop bar; the stop bar is used to fix the position of the incubation plate in the incubation chamber to ensure the stability of the incubation plate. The stop bar is at a distance from the upper edge of the incubation chamber.

Further, two or more incubation holes are provided, and a single brain slice/tissue slice is placed in a single incubation hole, and are taken separately when taking them. The setting of several incubation holes effectively solves the problems of mixing brain slices/tissue slices together, easily overlapping, and poor feeding. disturbance problem.

Further, the separator comprises a partition plate 1 located in the incubation chamber, a partition plate 2 located in the oxygen passage, and a support plate arranged on the inner wall of the oxygen passage; the partition plate 1 and the partition plate 2 are perpendicular to each other, and the partition plate The first partitions the incubation chamber, the second partition partitions the oxygen passage, and the incubation solution is placed in the chamber where the brain slices/tissue slices are placed; the incubation plate is provided with a through hole for accommodating the first partition; when in use, the second partition is placed On the upper end of the annular plate, the first partition plate is located in the incubation chamber, the through hole is aligned with the first partition plate, the incubation plate moves downward, and the first partition plate partitions the incubation plate.

Further, a partition plate 3 is arranged in the area where the brain slices/tissue slices are not planned to be incubated to cover the incubation holes of the brain slices/tissue slices that are not planned to be incubated.

Further, a temperature sensing sheet is set in the oxygen passage and/or the incubation chamber to monitor the temperature of the incubation solution in real time; avoiding the problem that only the temperature of the water bath can be monitored when the temperature is controlled by the water bath method, which is the most suitable for brain slices/tissue slices. Incubation provides more precise temperature conditions.

Further, the temperature control device includes a first area for accommodating the incubation device body and the water bath, and a second area located below the first area, the first area and the second area are separated by a partition baffle; in the second area The inside of the area is provided with an adjustment control for adjusting the water level of the water bath in the first area; this setting with the water level adjustment of the water bath can ensure that after the brain slices/tissue slices are continuously taken, the incubating fluid in the incubation chamber decreases, If the incubation requirements cannot be met, at this time, directly increase the water level of the existing water bath to meet the water bath requirements, and avoid adding a new incubation solution to the incubation chamber. On the one hand, the temperature environment will change, which is not conducive to brain slices/ Incubation of tissue slices, on the other hand, will disturb the position of the placed brain slices/tissue slices, and at the same time, it is also to avoid the situation that the water level of the water bath is lower than the incubation solution in the incubation chamber and the incubator is tilted, which will affect the Normal incubation of brain slices/tissue slices.

Further, the adjustment control includes: a moving plate located in the second area, a support frame supporting the moving plate, and an operation part arranged on the moving plate and the support frame; connecting plates are arranged at the lower ends of both sides of the moving plate, and the support frame is provided with The connecting plate extends into and moves the connecting groove; the supporting frame is fixed in the second area; the operation part controls the connecting plate and the moving plate to move relative to the supporting frame, and the water bath moves the upper part of the plate in the first area and the second area . A heating wire is set at the top of the moving plate to ensure the temperature of the water bath.

Further, slot holes are arranged up and down the separating baffle, so that the upper space of the moving plate in the second area is connected with the first area; when the water level of the water bath liquid drops, the moving plate moves, and the water bath liquid passes through the slot holes. Move to ensure the water level line; when the water level of the water bath is too high, the moving plate moves down, and the water bath moves up through the slot hole.

Further, a connecting block 1 is set above the separating baffle, and a matching connecting block 2 is also set at the lower end of the incubation device body; the connecting block 1 and the connecting block 2 are used to stably fix the incubation device body in the first area to avoid The device body is given an upward movement. The first connecting block is arranged in an inverted L-shape or T-shape or other shapes that can play a blocking role above, and the second connecting block matches the shape of the first connecting block.

Further, the operating part includes a connecting shaft 1 arranged between the support frames on both sides, a connecting shaft 2 arranged between the connecting plates on both sides, a rotating wheel 1 is arranged on the outer side of the connecting shaft 1, and a rotating wheel is arranged on the outer side of the connecting shaft 2 at a corresponding position. Wheel 2, the relative positions of the rotating wheel 1 and the rotating wheel 2 are fixed; the center of the connecting shaft 1 does not coincide with the center of the rotating wheel 1, and the center of the connecting shaft 2 does not coincide with the center of the rotating wheel 2; the connecting shaft 1 is movable with the support frame connection, the connecting shaft two is movably connected with the connecting plate; a driving part is arranged on one side or both sides of the connecting shaft one; the connecting shaft one and the rotating wheel one are driven to rotate by operating the driving part, and the rotating wheel one and the rotation drive the rotating wheel two and The connecting shaft 2 rotates. Since the rotating wheel 1 and the rotating wheel 2 are eccentrically arranged relative to the connecting shaft 1 and the connecting shaft 2 respectively, when the rotating wheel 1 and the rotating wheel 2 rotate relative to each other, they will drive the connecting plate and the moving plate to move up and down. . The diameter of the rotating wheel 1 and the rotating wheel 2 are the same.

Further, the two ends of the rotating wheel 1 or the rotating wheel 2 are provided with raised rings, which just accommodate the rotating wheel 2 or the rotating wheel 1; this arrangement ensures the stability of the rotating wheel 1 and the rotating wheel 2 when they rotate with each other.

Further, two of the first rotating wheel and the second rotating wheel are respectively arranged and located symmetrically at both ends, so as to ensure the stability of the connecting shaft one and the second connecting shaft when they rotate.

Further, a gear 1 is arranged on the outer side of the connecting shaft 1, a gear 2 is arranged on the outer side of the connecting shaft 2, and the gear 1 and the gear 2 are meshed with each other; It is mapped to a point horizontally with the center of the rotating wheel 2; the setting of gear 1 and gear 2 effectively realizes the self-locking effect.

Further, the driving part is a rotating handle arranged on one side or both sides of the connecting shaft, and the manual rotation is convenient for control.

Alternatively, the driving part is a motor arranged on one side or both sides of the connecting shaft, and a fixing frame supporting the motor is arranged outside the motor; a protective box is arranged on one or both sides of the temperature control device; The control method saves time and effort and completes automation.

Further, a control element is provided on the outer surface of the temperature control device, and the control element is provided with a temperature adjustment button, a constant temperature indicator light, a heating indicator light and a time display screen for controlling the temperature of the water bath.

Further, the control part is also provided with an operation key for controlling the start/stop of the motor, which is convenient for operation.

The beneficial effects of the present invention are as follows: 1. By setting up an incubation plate with several incubation holes, and setting up a closed-loop mouth-shaped oxygen passage and incubation chamber, the floating and overlapping of brain slices/tissue slices and the degree of oxygenation of the incubation solution are better solved. It can fully maintain the activity of cells in the isolated brain slices/tissue slices, improve the survival rate, and then obtain good experimental results; 2. Set up an isolation piece in the incubation chamber to divide the incubation chamber into two spaces, with a unique separation double The chamber can simultaneously incubate samples from different sources, and can also process samples from the same source in different ways, which solves the problem that the existing brain slice incubation device has only one incubation tank and cannot compare and observe the two experimental treatment conditions at the same time; It also overcomes the problem of wasting the incubation solution when incubating a single brain slice/tissue slice; 3. By setting the temperature control device for the incubation device body, and also setting the height of the water bath to adjust the liquid level of the water bath, the water bath outside the incubation device is guaranteed. The water level line is stable, which improves the survival rate and activity of the incubated diapers; in addition, setting a temperature sensor in the incubation chamber and/or the oxygen channel can monitor the temperature of the incubation solution, solving the problem that the current water bath can only monitor the temperature of water. The problem, and the real impact of the rewarming incubation effect is the incubation solution.

Description of drawings

1 is a schematic diagram of the overall structure of the incubation device body of the present invention;

2 is a schematic diagram of the overall structure of the incubation device body of the present invention without a cover plate;

3 is a front perspective view of the incubation device body of the present invention without a cover plate;

4 is an enlarged schematic view of the upper end of the incubation device body of the present invention;

Fig. 5 is the top view structure schematic diagram of the incubation plate of the present invention;

Fig. 6 is the bottom view structure schematic diagram of the incubation plate of the present invention;

FIG. 7 is a perspective view of the overall structure of the incubation device body provided with the isolation member in the incubation chamber of the present invention;

FIG. 8 is a partial structural cross-sectional view of the main body of the incubation device in which the isolation member is arranged in the incubation chamber of the present invention;

9 is a schematic structural diagram of an incubation plate and a spacer of the present invention;

10 is a schematic structural diagram of a temperature control device provided outside the incubation device body of the present invention;

11 is a perspective view of the structure of the temperature control device provided outside the incubation device body of the present invention;

12 is an enlarged structural cross-sectional view of the lower end of the temperature control device of the present invention;

13 is an enlarged structural schematic diagram of the lower end of the incubation device body and the adjustment control (the operation part is set as a rotating handle) according to the present invention;

14 is a schematic structural diagram of an incubation device body and a temperature control device (a control member and a protective box are provided outside the temperature control device) of the present invention;

FIG. 15 is a schematic structural diagram of the adjusting control (operating part is set as a motor) according to the present invention;

In the figure, 1. Incubation device body; 11. Incubation chamber; 12. Oxygen channel; 13. Oxygen supply pipeline; 14. Incubation plate; 15. Incubation hole; 16. Incubation net; 17. Cover plate; 18. Blocking bar ; 19, partition plate one; 110, partition plate two; 111, support plate; 112, through hole; 113, partition plate three; 114, temperature sensing sheet; 2, temperature control device; 22, second area; 23, partition baffle; 24, moving plate; 25, support frame; 26, connecting plate; 27, heating wire; 28, connecting block one; 29, connecting block two; 210, connecting shaft one; 211, connecting shaft two; 212, rotating wheel one; 213, rotating wheel two; 214, gear one; 215, gear two; 216, rotating handle; 217, motor; 218, fixing frame; 219, protective box; 220, control pieces.

Detailed ways

The technical solutions in the embodiments of the present invention will be clearly and completely described below through specific specific embodiments. Obviously, the described embodiments are only a part of the embodiments of the present invention, not all of the embodiments. Those skilled in the art can use the Other advantages and functions of the present invention are easily understood from the contents disclosed in the specification. The present invention can also be implemented or applied through other different specific embodiments. If there is no conflict, the following embodiments and the features in the embodiments can be combined with each other. Based on the embodiments of the present invention, those of ordinary skill in the art can All other embodiments obtained without creative work fall within the protection scope of the present invention.

Example 1

Please refer to FIG. 1 to FIG. 3 , a convenient-to-use porous brain slice incubation device in this embodiment includes an incubation device body 1; an incubation chamber 11 and an oxygen passage 12 are arranged in the incubation device main body 1, and the oxygen passage 12 is connected to the incubation device body 1. The chamber 11 is connected, and the oxygen passage 12 is connected to the oxygen supply pipeline 13; the incubation chamber 11 is provided with an incubation plate 14, and the incubation plate 14 is movably arranged inside the incubation chamber 11; the incubation plate 14 is provided with at least one incubation hole 15, below the incubation hole 15 An incubation net 16 for carrying brain slices/tissue slices is arranged; a spacer is arranged in the incubation chamber 11, and the spacer divides the inside of the incubation chamber 11 into different spaces; a temperature control device 2 is arranged outside the incubation device body 1 , the temperature control device 2 still uses the principle of the water bath method; because incubation in a water bath can keep the temperature well, and the temperature is controlled and heated evenly to ensure a suitable temperature environment; in this way, the brain slices/tissue slices are placed in the In the incubation hole 15, the oxygen supply pipeline 13 and the oxygen passage 12 are used to supply oxygen to the brain slice/tissue slice and incubate; the isolation element can effectively avoid wasting the incubation solution when incubating a single brain slice.

Example 2

Please refer to FIGS. 1 to 6 , on the basis of Embodiment 1, the following specific settings are performed on the incubation device body 1 in this embodiment: a cover plate 17 is provided on the upper end of the incubation device body 1 , and the cover plate 17 covers the incubation device body 1 to ensure that The tightness of the incubation chamber 11 and the oxygen passage 12. The shape of the incubation device body 1 is not limited. In this embodiment, the shape of the incubation device body 1 is set as a cube, but the shape of the incubation device body 1 can also be set as a cylinder or a shape that can be implemented smoothly. The shape of the incubation hole 15 is also not limited. The incubation hole 15 in this embodiment is a square, and may also be a circle or other shapes that are convenient for use. The shapes of the incubation chamber 11 and the incubation plate 14 are matched. In this embodiment, the shapes of the incubation chamber 11 and the incubation plate 14 are both square, and the shapes of the incubation chamber 11 and the incubation plate 14 may also be circular or other convenient shapes. shape, which is not limited.

The first end of the oxygen passage 12 is arranged at the lower end of the incubation chamber 11, the second end of the oxygen passage 12 is arranged at the left end or the right end of the incubation chamber 11, and the oxygen passage 12 and the incubation chamber 11 form a closed-loop mouth shape; in this embodiment Among them, the oxygen supply pipeline 13 is arranged in any section of the oxygen channel 12 parallel to the center line of the incubation chamber 11; when in use, the oxygen supply pipeline 13 is connected to the oxygen supply equipment, and the oxygen enters the oxygen channel 12 and moves upwards and moves along the oxygen channel 12. Enter into the incubation chamber 11 to provide oxygen for the incubation of brain slices/tissue slices. This setting can drive the flow of the incubation solution, but the oxygen circuit is not on the incubation plate 14, which prevents the blow-off force on the brain slices/tissue slices. A blocking bar 18 is provided on the inner side wall of the incubation chamber 11 , and the incubation plate 14 is placed on the upper end of the blocking bar; The stop bar is at a distance from the upper edge of the incubation chamber 11 .

Two or more incubation holes 15 are arranged, and a single brain slice/tissue slice is placed in a single incubation hole 15, and are taken separately when taking them. The arrangement of the several incubation holes 15 effectively solves the problems of mixing brain slices/tissue slices together, easily overlapping, and poor feeding. disturbing problems. The incubation net 16 is made of nylon wire to ensure normal incubation of the brain slices/tissue slices; the incubation plate 14 is made of acrylic material.

A control member 220 is provided on the outer surface of the temperature control device 2, and the control member 220 is also provided with an operation key for controlling the start/stop of the motor 217, which is convenient for operation.

Example 3

Referring to FIGS. 7-9 , on the basis of Embodiment 2, this embodiment specifically sets the spacer: the spacer in this embodiment divides the interior of the incubation chamber 11 into different spaces. The spacer includes a partition plate 19 located in the incubation chamber 11, a partition plate 2 110 located in the oxygen passage 12, and a support plate 111 arranged on the inner wall of the oxygen passage 12; the partition plate 1 19 and the partition plate 2 110 are perpendicular to each other , the partition plate one 19 partitions the incubation chamber 11, the partition plate two 110 partitions the oxygen passage 12, and the incubation solution is placed in the chamber where the brain slices/tissue slices are placed; Through hole 112; when in use, the second partition plate 110 is placed on the upper end of the annular plate, the first partition plate 19 is located in the incubation chamber 11, and then the through hole 112 is aligned with the partition plate one 19, the incubation plate 14 moves down, and the separation Partition one 19 partitions the incubation plate 14.

A partition plate 3 113 is arranged in the area where the brain slices/tissue slices are not planned to be incubated to cover the incubation holes 15 of the brain slices/tissue slices that are not planned to be incubated. In this embodiment, the oxygen passage 12 is set to be circular, the support plate 111 is set to a ring plate, the brain slice/tissue slice is incubated on the side away from the right side of the mouth-shaped oxygen passage 12, and the second partition plate 110 is set to a half Circular, the length of the upper part of the partition plate 19 at the upper end of the incubation plate 14 is smaller than the length of the lower part of the incubation plate 14, so that the incubation solution can flow into the side where the brain slices/tissue slices are incubated, and the partition plate 113 blocks the incubation solution Flow into the incubation chamber 11 from the oxygen passage 12 , and block the incubation liquid from flowing into the incubation chamber 11 from the incubation hole 15 .

A temperature sensing sheet 114 is arranged in the oxygen passage 12 and/or the incubation chamber 11 to monitor the temperature of the incubation solution in real time; to avoid the problem of only monitoring the temperature of the water bath when the temperature is controlled by the water bath method at present, it is the most suitable for brain slices/tissue slices. Incubation provides more precise temperature conditions.

Example 4

10-15, on the basis of Embodiment 1, 2 or 3, this embodiment specifically sets the temperature control device 2: the temperature control device 2 includes a first temperature control device for accommodating the incubation device body 1 and the water bath. The area 21, and the second area 22 located below the first area 21, the first area 21 and the second area 22 are separated by a partition baffle 23; Adjustment of the inner water level line; the setting of the water level line with the adjustment water bath can ensure that after the brain slices/tissue slices are continuously taken, the incubation liquid in the incubation chamber 11 is reduced and cannot meet the incubation requirements. The method of directly raising the water level of the existing water bath can meet the needs of the water bath and avoid adding a new incubation solution to the incubation chamber 11 again. On the one hand, the temperature environment will change, which is not conducive to the incubation of brain slices/tissue slices. It will also disturb the position of the placed brain slices/tissue slices. At the same time, it is also to avoid the situation that the water level of the water bath is lower than the incubation solution in the incubation chamber 11, and the incubator is tilted, thus affecting the brain slices/tissue slices. normal incubation.

The adjustment control includes: a moving plate 24 located in the second area 22, a supporting frame 25 supporting the moving plate 24, and an operation part arranged on the moving plate 24 and the supporting frame 25; The frame 25 is provided with a connecting groove for the connecting plate 26 to extend into and move; the supporting frame 25 is fixed in the second area 22; The part above the moving plate 24 in the area 21 and the second area 22. An electric heating wire 27 is arranged on the upper end of the moving plate 24 to ensure the temperature of the water bath.

Slot holes are arranged up and down the separating baffle, so that the upper end space of the moving plate 24 in the second area 22 is connected with the first area 21; when the water level of the water bath liquid drops, the moving plate 24 moves up, and the water bath liquid passes through the slot holes. Move to ensure the water level line; when the water level of the water bath is too high, the moving plate 24 moves down, and the water bath moves up through the slot hole.

A connecting block 1 28 is set above the separating baffle 23, and a matching connecting block 2 29 is also set at the lower end of the incubation device body 1; the incubation device body 1 is stably fixed in the first area by using the connecting block 1 28 and the connecting block 2 29 21, to avoid upward movement of the body of the imparting device. The first connecting block 28 is set in an inverted L-shape or T-shape or other shapes whose upper part can play a blocking role, and the second connecting block 29 matches the shape of the connecting block one 28 .

The operation part includes a connecting shaft 1 210 arranged between the support frames 25 on both sides, a connecting shaft 2 211 arranged between the connecting plates 26 on both sides, a rotating wheel 1 212 is arranged on the outer side of the connecting shaft 1 210, and the outer side of the connecting shaft 2 211 corresponds to The position of the second rotating wheel 213 is set, and the relative positions of the first rotating wheel 212 and the second rotating wheel 213 are fixed; the center of the connecting shaft one 210 does not coincide with the center of the rotating wheel one 212, and the center of the connecting shaft two 211 and the second rotating wheel 213. The connecting shaft 1 210 is movably connected with the support frame 25, and the connecting shaft 2 211 is movably connected with the connecting plate 26; a driving part is arranged on one or both sides of the connecting shaft 1 210; the connecting shaft 1 210 and The rotation of the rotating wheel 1 212, the rotating wheel 1 212 and the rotation drive the rotating wheel 2 213 and the connecting shaft 2 211 to rotate, because the rotating wheel 1 212 and the rotating wheel 2 213 are eccentric relative to the connecting shaft 1 210 and the connecting shaft 2 211 respectively. Therefore, when the first rotating wheel 212 and the second rotating wheel 213 rotate relative to each other, they will drive the connecting plate 26 and the moving plate 24 to move up and down. The first rotating wheel 212 and the second rotating wheel 213 have the same diameter.

The two ends of the first rotating wheel 212 or the second rotating wheel 213 are provided with raised rings, which just accommodate the second rotating wheel 213 or the second rotating wheel 212; this arrangement ensures the stability of the rotating wheel one 212 and the second rotating wheel 213 when they rotate with each other. Two rotating wheels 212 and two rotating wheels 213 are provided respectively, and are located symmetrically at both ends to ensure the stability of the first connecting shaft 210 and the second connecting shaft 211 when they rotate.

A gear one 214 is arranged on the outside of the connecting shaft one 210, and a gear two 215 is arranged on the outside of the connecting shaft two 211. The gear one 214 and the gear two 215 mesh with each other; the center of the gear one 214 and the center of the rotating wheel one 212 are horizontally mapped to a point, The circle center of the second gear 215 and the circle center of the second rotating wheel 213 are horizontally mapped to a point; the setting of the first gear 214 and the second gear 215 effectively realizes the self-locking effect.

The driving part is a rotating handle 216 arranged on one side or both sides of the connecting shaft 1 210 , and the manual rotation is convenient for control. Alternatively, the driving part is a motor 217 arranged on one side or both sides of the connecting shaft 1 210, and a fixing frame 218 supporting the motor 217 is arranged outside the motor 217; Protective box 219; the way of electric control saves time and effort, and completes automation.

A control part 220 is provided on the outer surface of the temperature control device 2, and the control part 220 is provided with a temperature adjustment button, a constant temperature indicator light, a heating indicator light and a time display screen for controlling the temperature of the water bath.

When in use, when incubating, gently place the brain slices/tissue slices on the incubation net 16, cover the main body 1 of the incubation device and place the lid on the temperature control device 2 that has reached the specified temperature to start incubation. Frequently take brain slices/tissue slices, after the water level of the incubation solution drops, operate the operation part to move the moving plate 24 up, the water level of the water bath rises, and continue the incubation. The structure is simple, easy to disassemble and wash, and the incubation net 16 can be replaced after each use. It can also be carefully washed with distilled water after use, and soaked in distilled water (with a few drops of HCL), which can be used repeatedly for several times without changing the incubation net every time. 16.

The descriptions of the above-mentioned embodiments are only for understanding the present invention. It should be pointed out that for those of ordinary skill in the art, without departing from the principle of the present invention, several improvements can also be made to the present invention, and these improvements will also fall within the protection scope of the claims of the present invention.

Claims (9)

1. A porous brain piece incubation device convenient to use comprises an incubation device body and is characterized in that; an incubation chamber and an oxygen passage are arranged in the incubation device body, the oxygen passage is communicated with the incubation chamber, and the oxygen passage is connected with an oxygen supply pipeline; the incubation chamber is internally provided with an incubation plate which is movably arranged in the incubation chamber; the incubation plate is provided with at least one incubation hole, and an incubation net for bearing brain slices/tissue slices is arranged below the incubation hole; arranging a partition piece in the incubation chamber, wherein the partition piece divides the interior of the incubation chamber into different spaces; a temperature control device is arranged outside the incubation device body, and the temperature control device still uses the principle of a water bath method; the temperature control device comprises a first area for accommodating the incubation device body and the water bath liquid and a second area positioned below the first area, and the first area and the second area are separated by a separation baffle; a regulating part for regulating the water level of the water bath liquid in the first area is arranged in the second area; this kind of setting up of water line with adjustment water bath liquid can guarantee to take brain piece/tissue piece after continuously, it reduces to hatch indoor hatching liquid, can not satisfy the condition of hatching the requirement, the mode through the water line of the current water bath liquid of direct improvement satisfies the water bath demand this moment, avoid adding new hatching liquid to hatching indoor again, temperature environment can change on the one hand, be unfavorable for the hatching of brain piece/tissue piece, on the other hand also can be to the position emergence disturbance of the brain piece/tissue piece of having placed, also be simultaneously in order to avoid appearing the water bath liquid water line and be less than hatching indoor hatching liquid, the condition of slope takes place for hatching the ware, thereby influence the normal hatching of brain piece/tissue piece.

2. The conveniently used multi-hole brain slice incubating device according to claim 1, wherein a cover plate is arranged at the upper end of the incubating device body; furthermore, the first end of the oxygen passage is arranged at the lower end of the incubation chamber, the second end of the oxygen passage is arranged at the left end or the right end of the incubation chamber, and the oxygen passage and the incubation chamber form a closed-loop square shape.

3. The multi-well brain piece incubation device for convenient use according to claim 1, wherein two or more incubation wells are provided, and a single brain piece/tissue piece is placed in a single incubation well.

4. The multi-hole brain piece incubation device convenient to use of any one of claims 1-3, wherein the partition comprises a first partition plate located in the incubation chamber, a second partition plate located in the oxygen passage, and a support plate arranged on the inner wall of the oxygen passage; the first partition plate and the second partition plate are perpendicular to each other, the first partition plate partitions the interior of the incubation chamber, the second partition plate partitions the oxygen passage, and the incubation liquid is placed in the chamber for placing the brain slice/tissue slice; the incubation plate is provided with a through hole for accommodating the first partition plate; furthermore, a third partition plate is arranged in the area where the brain piece/tissue piece is not planned to be incubated, and covers the incubation hole where the brain piece/tissue piece is not planned to be incubated.

5. The conveniently used multi-hole brain piece incubation device according to any of claims 1-3, wherein a temperature sensing piece is arranged in the oxygen passage and/or the incubation chamber to monitor the temperature of the incubation liquid in real time; the problem that only the temperature of the water bath solution can be monitored when the temperature is controlled by adopting a water bath method at present is solved, and more accurate temperature conditions are provided for the incubation of brain slices/tissue slices.

6. The multi-well brain piece incubation device for convenient use according to claim 1, wherein the adjustment member comprises: the movable plate is positioned in the second area, the support frame supports the movable plate, and the operating parts are arranged on the movable plate and the support frame; the lower ends of the two sides of the movable plate are provided with connecting plates, and the support frame is provided with connecting grooves for the connecting plates to extend into and move; the support frame is fixed in the second area; the operation part controls the connecting plate and the moving plate to move relative to the supporting frame, and the water bath liquid moves the upper part of the moving plate in the first area and the second area. Furthermore, the upper end of the movable plate is provided with an electric heating wire to ensure the temperature of the water bath liquid.

7. The multi-hole brain piece incubation device convenient to use of claim 6, wherein slotted holes are arranged on the upper part and the lower part of the separation baffle plate in a penetrating way, so that the space at the upper end of the moving plate in the second area is communicated with the first area; when the water level line of the water bath liquid descends, the moving plate moves upwards, the water bath liquid moves upwards through the slotted hole, and the water level line is ensured; when the water line of the water bath liquid is too high, the moving plate moves downwards, and the water bath liquid moves upwards through the slotted hole; furthermore, a first connecting block is arranged above the separation baffle, and a second connecting block matched with the first connecting block is also arranged at the lower end of the incubation device body; the incubation device body is stably fixed in the first area by the aid of the first connecting block and the second connecting block, and the situation that the incubation device body moves upwards is avoided.

8. The conveniently-used porous brain slice incubating device according to claim 7, wherein the operating part comprises a first connecting shaft arranged between the supporting frames at two sides and a second connecting shaft arranged between the connecting frames at two sides, a first rotating wheel is arranged at the outer side of the first connecting shaft, a second rotating wheel is arranged at the corresponding position at the outer side of the second connecting shaft, and the relative positions of the first rotating wheel and the second rotating wheel are fixed; the circle center of the first connecting shaft is not coincident with the circle center of the first rotating wheel, and the circle center of the second connecting shaft is not coincident with the circle center of the second rotating wheel; the first connecting shaft is movably connected with the support frame, and the second connecting shaft is movably connected with the connecting plate; a driving part is arranged on one side or both sides of the first connecting shaft; the first connecting shaft and the first rotating wheel are driven to rotate through the operation driving portion, the first rotating wheel and the second rotating wheel are driven to rotate through rotation, and the first rotating wheel and the second rotating wheel are eccentrically arranged relative to the first connecting shaft and the second connecting shaft respectively, so that the first rotating wheel and the second rotating wheel can drive the connecting plate and the moving plate to move up and down when rotating relatively.

9. The conveniently-used porous brain slice incubating device according to claim 8, wherein a first gear is arranged on the outer side of the first connecting shaft, a second gear is arranged on the outer side of the second connecting shaft, and the first gear and the second gear are meshed with each other; the center of the first gear and the center of the first rotating wheel are horizontally mapped into a point, and the center of the second gear and the center of the second rotating wheel are horizontally mapped into a point; the self-locking effect is effectively realized by the arrangement of the first gear and the second gear.

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