CN114196529A - Intelligent plant pathogenic bacterium culture system and culture method thereof - Google Patents
Intelligent plant pathogenic bacterium culture system and culture method thereof Download PDFInfo
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- CN114196529A CN114196529A CN202111617271.8A CN202111617271A CN114196529A CN 114196529 A CN114196529 A CN 114196529A CN 202111617271 A CN202111617271 A CN 202111617271A CN 114196529 A CN114196529 A CN 114196529A
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- 238000012136 culture method Methods 0.000 title claims abstract description 10
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- 238000010438 heat treatment Methods 0.000 claims description 9
- 238000012258 culturing Methods 0.000 claims description 7
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- 238000006073 displacement reaction Methods 0.000 claims description 3
- 241000894006 Bacteria Species 0.000 abstract description 17
- 238000002474 experimental method Methods 0.000 abstract description 14
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Abstract
The invention discloses an intelligent plant pathogenic bacteria culture system and a culture method thereof in the field of plant bacteria culture, and the system comprises a support column fixedly arranged on a rack, wherein a light source is arranged on the support column; the bearing table is rotatably arranged along the axial direction of the support column at intervals, an object stage is arranged on the bearing table, a sliding groove is vertically formed in the bearing table, and the object stage is vertically and slidably arranged on the sliding groove; the objective table side is provided with dimming mechanism. The culture system can automatically shield the circumferential side wall of the culture dish and prevent external light from entering the culture dish through the circumferential side wall of the culture dish; can also reflect external light to the central authorities of culture dish through the reflector for plant bacteria breeds in the middle of the culture dish, the observation of being convenient for. In addition, the reflecting angle of the reflector can be automatically adjusted according to the height of the liquid level in the culture dish, so that the reflector can always reflect light to the center of the liquid level of the culture dish, subsequent observation is facilitated, and the experiment efficiency is improved.
Description
Technical Field
The invention belongs to the field of plant bacteria culture, and particularly relates to an intelligent plant pathogenic bacteria culture system and a culture method thereof.
Background
Fungal mycelia in diseased plant tissues generally recover growth and reproduction, except for individual species, if given appropriate environmental conditions. The isolation of plant pathogenic bacteria refers to the separation of pathogenic fungi from other miscellaneous fungi from the tissues of affected plants by artificial culture, and the separation of pathogenic bacteria from host plants, and then the purification of the separated pathogenic bacteria in a suitable environment, which is generally called the isolation culture of plant pathogenic bacteria. The separation of plant pathogenic fungi generally adopts a tissue separation method, namely, a small piece of diseased tissue is cut, and is transferred to an artificial culture medium for culture after surface disinfection, sterilization and water washing. Usually, the culture dish for carrying the culture medium is made of glass, has light transmittance, and is convenient for experimenters to observe the culture state in the culture dish at any time in experiments. But in the actual plant bacteria culture process to some having phototaxis, because the light source is in the outside of culture dish, the light that the light source sent can enter into the culture dish through the lateral wall of culture dish in, plant bacteria can be because having phototaxis and the adherence, plant bacteria will breed in a large number on the inside wall of culture dish like this for follow-up experimenter's observation is very inconvenient, influences the efficiency of experiment and the accuracy of observing.
The invention provides an intelligent plant pathogenic bacteria culture system and a culture method thereof, wherein the culture system not only can automatically shield the circumferential side wall of a culture dish and prevent external light from entering the culture dish through the circumferential side wall of the culture dish to cause phototaxis adherence of plant bacteria; can also reflect external light to the central authorities of culture dish through the reflector for plant bacteria breeds in the middle of the culture dish, the observation of being convenient for. In addition, the reflecting angle of the reflector can be automatically adjusted according to the height of the liquid level in the culture dish, so that the reflector can always reflect light to the center of the liquid level of the culture dish, subsequent observation is facilitated, and the experiment efficiency is improved.
Disclosure of Invention
The invention aims to provide an intelligent plant pathogenic bacteria culture system and a culture method thereof, and aims to solve the problems of the prior art in the background technology.
In order to achieve the purpose, the invention provides the following technical scheme: an intelligent plant pathogenic bacteria culture system comprises a support column fixedly arranged on a rack, wherein a light source is arranged on the support column; the carrying platform is rotatably installed along the axial direction of the supporting column at intervals, an object stage for placing a culture dish is arranged on the carrying platform, a sliding groove is vertically formed in the carrying platform, the object stage is vertically and slidably installed on the sliding groove, and a first compression spring is arranged between the object stage and the bottom of the sliding groove; a light adjusting mechanism is arranged beside the objective table and comprises a reflector, and the reflector is used for reflecting light emitted by a light source to the center of the liquid level of the paper culture dish; the light adjusting mechanism adjusts the reflection angle of the reflector according to the displacement of the objective table.
As a further scheme of the invention, the dimming mechanism comprises a support rod fixedly arranged beside the objective table, a rack is fixedly arranged at the edge of the bottom surface of the objective table and positioned beside the support rod, a first rotating shaft is rotatably arranged at the lower end in the support rod, and a second rotating shaft is rotatably arranged at the upper end in the support rod; a gear and a first synchronizing wheel are fixedly arranged on the first rotating shaft, and the gear is meshed with the rack; a second synchronizing wheel is fixedly arranged on the second rotating shaft, and the first synchronizing wheel is in synchronous transmission connection with the second synchronizing wheel; the reflector is fixedly mounted on the second rotating shaft.
As a further aspect of the present invention, the reflective mirror is a convex mirror.
As a further scheme of the invention, a guide mechanism is arranged on the circumferential direction of the objective table; the correcting mechanism comprises a correcting plate which is arranged on the bearing table in a sliding mode along the radial direction of the object table; a fixed plate fixedly arranged on the bearing table is arranged on one side, away from the objective table, of the guide plate, and a second compression spring is arranged between the guide plate and the fixed plate; one side of leading positive plate near the objective table is provided with blocks the pole, block the pole and hinder leading positive plate to the direction removal near the objective table, block the vertical slidable mounting of pole on the plummer, block pole and objective table fixed connection.
In a further aspect of the present invention, the guiding plate is arc-shaped, and covers the circumferential surface of the culture dish after guiding.
As a further scheme of the invention, the correcting plate is processed by leather skin.
As a further scheme of the invention, a heating strip is arranged on the inner side wall of the sliding groove and used for controlling the temperature of the culture dish.
As a further scheme of the invention, the light source is a strip-shaped lamp tube which is arranged at intervals along the circumferential direction of the support column.
As a further scheme of the invention, the strip-shaped lamp tube has adjustable brightness.
As a further scheme of the invention, the support column is arranged at the center in the incubator, the wall of the incubator is provided with a vent, and a filter screen is arranged at the vent.
The invention also provides an intelligent plant pathogenic bacteria culture method, which mainly comprises the following steps:
s1: preparing a culture medium required for culturing plant pathogenic bacteria, and placing the culture medium in a culture dish;
s2: inoculating the separated plant pathogenic bacteria to a culture dish in S1;
s3: placing the culture dish in the S2 on an object stage in an incubator, and adjusting the required light intensity and temperature;
s4: and taking out the culture dish after culturing for 2-3 days.
Compared with the prior art, the invention has the beneficial effects that:
1. the invention can automatically shield the circumferential side wall of the culture dish, and prevent external light from entering the culture dish through the circumferential side wall of the culture dish to cause phototaxis adherence of plant bacteria; can also reflect external light to the central authorities of culture dish through the reflector for plant bacteria breeds in the middle of the culture dish, the observation of being convenient for. In addition, the reflecting angle of the reflector can be automatically adjusted according to the height of the liquid level in the culture dish, so that the reflector can always reflect light to the center of the liquid level of the culture dish, subsequent observation is facilitated, and the experiment efficiency is improved.
2. The invention can automatically adjust the reflection angle of the reflector according to the height of the liquid level in the culture dish. According to the invention, the height of the culture dish placed on the objective table is judged according to the position height of the objective table, and the deflection angle of the reflector is driven by the downward moving distance of the objective table, so that the reflector can be automatically adjusted along with the height change of the culture dish, the light rays reflected by the reflector can always follow the culture dish, the cultured plant pathogenic bacteria can be greatly propagated in the center of the culture dish, and the observation of subsequent workers is facilitated.
3. The embodiment that this embodiment provided is that three are led the positive board and are carried out radial movement and lead positive, three point centering for the location is more accurate. The culture dish positioning device judges whether the culture dish is placed on the objective table or not by judging whether the objective table moves downwards or not, and the downward movement of the objective table is utilized to trigger the guide plate to guide, so that the culture dish positioning device is simple in structure, convenient to use and accurate in positioning, and the experiment efficiency and the observation accuracy are improved. According to the invention, the shape of the correcting plate is set to be more in line with the arc shape of the peripheral surface of the culture dish, so that on one hand, the culture dish is more stably moved during correcting, and the culture dish is prevented from shaking during moving, so that a culture medium in the culture dish escapes, unnecessary waste is caused, and resources are saved; on the other hand leads the positive board with the arc and handles with leather skin, further carries out the shading to the circumference lateral wall of culture dish, avoids light to enter into the culture dish from this department, improves the light-proofness to culture dish circumference lateral wall, guarantees that the bacterium can not be because of light tends and the adherence for subsequent observation is very inconvenient, influences the efficiency of experiment and the accuracy of observing.
Drawings
In order to more clearly illustrate the technical solutions of the embodiments of the present invention, the drawings used in the description of the embodiments will be briefly introduced below, and it is obvious that the drawings in the following description are only some embodiments of the present invention, and it is obvious for those skilled in the art that other drawings can be obtained according to the drawings without creative efforts.
FIG. 1 is a schematic structural diagram of an intelligent plant pathogenic bacteria culture system according to the present invention;
FIG. 2 is a schematic view of the structure of the loading platform, the culture dish and the light adjusting mechanism of the present invention;
FIG. 3 is a schematic view of the present invention with the culture dish removed from FIG. 2;
FIG. 4 is an enlarged view of a portion A of FIG. 3 according to the present invention;
FIG. 5 is a cross-sectional view of the dimmer mechanism of the present invention;
FIG. 6 is a schematic structural diagram of a light adjusting mechanism and a guiding mechanism according to the present invention;
FIG. 7 is a schematic structural view of a sliding groove according to the present invention;
FIG. 8 is a cross-sectional view of a dimming mechanism and a polarization mechanism of the present invention;
FIG. 9 is an enlarged view of a portion B of FIG. 8 in accordance with the present invention;
FIG. 10 is a process flow diagram of the intelligent method for culturing plant pathogenic bacteria of the present invention.
In the drawings, the components represented by the respective reference numerals are listed below:
11-support column, 12-bearing table, 13-light source, 14-object stage, 15-sliding groove, 16-first compression spring, 21-reflector, 22-support rod, 23-rack, 24-first rotating shaft, 25-second rotating shaft, 26-gear, 27-first synchronizing wheel, 28-second synchronizing wheel, 31-guide plate, 32-fixing plate, 33-second compression spring, 34-blocking rod, 41-heating bar, 42-culture dish, 51-culture box, 52-ventilation opening and 53-filter screen.
Detailed Description
The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.
Referring to fig. 1-10, an intelligent plant pathogenic bacteria culture system includes a support column 11 fixedly disposed on a frame, wherein a light source 13 is disposed on the support column 11; the carrying platform 12 is rotatably installed along the axial direction of the supporting column 11 at intervals, an object stage 14 for placing a culture dish 42 is arranged on the carrying platform 12, a sliding groove 15 is vertically formed in the carrying platform 12, the object stage 14 is vertically and slidably installed on the sliding groove 15, and a first compression spring 16 is arranged between the object stage 14 and the bottom of the sliding groove 15; a light adjusting mechanism is arranged beside the object stage 14, the light adjusting mechanism comprises a reflecting mirror 21, and the reflecting mirror 21 is used for reflecting light emitted by the light source 13 to the center of the liquid level of the paper culture dish 42; the light adjusting mechanism adjusts the reflection angle of the mirror 21 according to the displacement of the stage 14.
In the actual process of culturing some plant bacteria with phototaxis, because the light source 13 is outside the culture dish 42, the plant bacteria adhere to the wall due to phototaxis, so that the subsequent observation is very inconvenient, and the efficiency of the experiment and the accuracy of the observation are affected. Therefore, as shown in fig. 3 and 8, the culture dish 42 inoculated with the plant pathogenic bacteria is placed on the stage 14 on the carrier 12, and the stage 14 is driven by the gravity of the culture dish 42 to move vertically downward in the sliding groove 15 to press the first compression spring 16. Because the culture dish 42 is placed on the objective table 14, the culture dish 42 can move down and get into the sliding tray 15 along with the objective table 14, so the circumferential side wall of the culture dish 42 has the shelter from the side wall of the sliding tray 15, the external light source 13 can not enter the culture dish 42 through the circumferential side wall of the culture dish 42, the phototaxis adherence of plant bacteria is avoided, the observation of subsequent experimenters is very inconvenient, and the efficiency of the experiment and the accuracy of the observation are affected. After the shielding of the circumferential side wall of the culture dish 42 is completed, in order to facilitate the experimenters to observe the cultivation condition of the plant pathogenic bacteria in the culture dish 42, it is preferable to enable the plant pathogenic bacteria to multiply in a large amount at the center of the culture dish, so as to provide favorable observation conditions for the subsequent experimenters to observe. Therefore, the light adjusting mechanism of the invention can reflect the light emitted by the light source 13 on the support column 11 to the center of the culture dish 42, so that the cultured plant pathogenic bacteria can be greatly propagated at the center of the culture dish 42, and the observation of the follow-up staff is facilitated.
As a further scheme of the present invention, the dimming mechanism includes a support rod 22 fixedly disposed beside the object stage 14, a rack 23 is fixedly disposed at an edge of a bottom surface of the object stage 14, the rack 23 is located beside the support rod 22, a first rotating shaft 24 is rotatably mounted at a lower end in the support rod 22, and a second rotating shaft 25 is rotatably mounted at an upper end; a gear 26 and a first synchronous wheel 27 are fixedly arranged on the first rotating shaft 24, and the gear 26 is meshed with the rack 23; a second synchronizing wheel 28 is fixedly arranged on the second rotating shaft 25, and the first synchronizing wheel 27 is in synchronous transmission connection with the second synchronizing wheel 28; the mirror 21 is fixedly mounted on a second rotation shaft 25.
As shown in FIG. 2, the present invention reflects the light source 13 emitted from the support post 11 to the liquid surface of the culture dish 42 by the reflector 21 provided beside the stage 14, so that the cultured phytopathogenic bacteria can multiply in the center of the culture dish 42. However, in the above-mentioned actual cultivation process, because the culture dish 42 moves in the sliding groove 15 along with the carrier 14, when different plant pathogenic bacteria are cultivated and different culture mediums are used, the weight of each cultivation of the culture dish 42 may be different, the distance that the culture dish 42 moves downwards along with the carrier 14 may also be different, the liquid level of the culture medium in the culture dish 42 may also be changed, and at this time, if a fixed reflection angle is set, the light reflected by the reflector 21 cannot be always guaranteed to be reflected at the midpoint of the culture medium in the culture dish 42, which may affect the observation of the subsequent workers. Therefore, the light adjusting mechanism provided by the invention can automatically adjust the reflection angle of the reflector 21 according to the liquid level height in the culture dish 42, so that the reflector 21 can always reflect light to the center of the liquid level of the culture dish 42, subsequent observation is facilitated, and the experiment efficiency is improved. As shown in fig. 8 and 9, when the culture dish 42 inoculated with the plant pathogenic bacteria is placed on the stage 14, the stage 14 slides the culture dish 42 vertically downward in the slide groove 15 by gravity. As shown in fig. 9, since the falling of the object stage 14 causes the rack 23 fixedly disposed on the object stage 14 to move downward, the rack 23 drives the gear 26 at the side to rotate counterclockwise, and the gear 26 drives the first rotating shaft 24 to rotate counterclockwise. As shown in fig. 8, the first rotating shaft 24 rotates counterclockwise, the second rotating shaft 25 also rotates counterclockwise through the synchronous transmission of the first synchronizing wheel 27 and the second synchronizing wheel 28, and the reflective mirror 21 fixedly mounted on the second rotating shaft 25 is adjusted in angle along with the rotation of the second rotating shaft 25, so that the reflection angle is increased, and the light reflected by the reflective mirror 21 can be deflected downward along with the downward movement of the stage 14, and always follows the center of the culture dish 42 placed on the stage 14. The invention can automatically adjust the reflection angle of the reflector 21 according to the height of the liquid level in the culture dish 42. According to the invention, the height of the culture dish 42 placed on the object stage 14 is judged according to the position height of the object stage 14, the reflecting mirror 21 is driven to deflect through the downward moving distance of the object stage 14, so that the reflecting mirror 21 can be automatically adjusted along with the height change of the culture dish 42, the reflected light can always follow the culture dish 42, the cultured plant pathogenic bacteria can be greatly propagated in the center of the culture dish 42, and the observation of subsequent workers is facilitated.
As shown in fig. 6, as a further aspect of the present invention, the reflective mirror 21 is a convex mirror. The convex mirror has the function of converging light rays, and the purpose of the arrangement is to reflect more light rays, ensure the illumination intensity of the culture dish 42 and provide a powerful guarantee for the cultivation of plant pathogenic bacteria. In addition, the light can generate heat, and the arrangement of the reflector 21 is favorable for preventing the heat in the cultivation environment from dissipating and is favorable for heat preservation.
As a further aspect of the present invention, a guiding mechanism is disposed in the circumferential direction of the stage 14; the correcting mechanism comprises a correcting plate 31 which is arranged on the bearing table 12 in a sliding mode along the radial direction of the object table 14; a fixed plate 32 fixedly arranged on the bearing table 12 is arranged on one side of the guide plate 31 far away from the object stage 14, and a second compression spring 33 is arranged between the guide plate 31 and the fixed plate 32; one side of the correcting plate 31 close to the objective table 14 is provided with a blocking rod 34, the blocking rod 34 blocks the correcting plate 31 from moving towards the direction close to the objective table 14, the blocking rod 34 is vertically and slidably mounted on the bearing table 12, and the blocking rod 34 is fixedly connected with the objective table 14.
In the actual use process of the present invention, the sizes of the bottom surfaces of the culture dishes 42 of different specifications may not completely coincide with the size of the supporting surface of the stage 14, so that the positions of the culture dishes 42 placed on the stage 14 are not fixed. In order to ensure that the light reflected by the reflector 21 can move along with the culture dish 42 and is always reflected at the central position of the culture dish 42, the culture dish 42 needs to be positioned at this moment, and the reflector 21 is prevented from being reflected on the side wall of the culture dish 42, so that the cultured bacteria adhere to the wall, the subsequent observation is inconvenient, and the experiment efficiency and the observation accuracy are affected. As shown in FIG. 4, the present invention also provides a centering mechanism for centering the culture dish 42 placed on the stage 14 so that the culture dish 42 can be positioned at the center of the stage 14. As shown in fig. 4 and 8, when the stage 14 moves downward due to gravity during operation, the stage 14 drives the blocking rod 34 to move downward, the blocking rod 34 cancels the blocking of the guiding plate 31, the guiding plate 31 moves toward the stage 14 under the driving of the second compression spring 33, and the guiding plate 31 contacts with the culture dish 42 placed on the stage 14 and is then guided to the center of the stage 14. The embodiment provided by the embodiment is that the three guide plates 31 perform radial movement guide and three-point centering, so that the positioning is more accurate. The invention judges whether the culture dish 42 is placed on the objective table 14 by judging whether the objective table 14 moves downwards, and the downwards movement of the objective table 14 is utilized to trigger the correcting plate 31 to correct, so that the structure is simple, the use is convenient, the positioning is accurate, and the efficiency of the experiment and the accuracy of observation are improved.
In a further embodiment of the present invention, the guiding plate 31 is arc-shaped, and the guiding plate 31 covers the circumferential surface of the culture dish 42 after guiding. As shown in fig. 8, the purpose of this arrangement is to make the shape of the alignment plate 31 conform to the shape of the peripheral surface of the culture dish 42, to make the movement of the culture dish 42 more stable during alignment, and to avoid the culture dish 42 from shaking during movement, so that the culture medium in the culture dish 42 escapes, which causes unnecessary waste and saves resources.
As a further aspect of the present invention, the correcting plate 31 is skinned by leather. The purpose of this setting is in order to further carry out the shading to the circumference lateral wall of culture dish 42, avoids during light enters into culture dish 42 from this department, improves the light-proofness to culture dish 42 circumference lateral wall, guarantees that the bacterium can not be because of light trend and adherence for subsequent observation is very inconvenient, influences the efficiency of experiment and the accuracy of observing.
In a further embodiment of the present invention, a heating bar 41 is disposed on an inner sidewall of the sliding groove 15, and the heating bar 41 is used for controlling the temperature of the culture dish 42. As shown in FIG. 7, in the actual cultivation of the plant pathogenic bacteria, the culture dish 42 needs to be heated to ensure an appropriate temperature because of the requirement of the environment temperature for the plant pathogenic bacteria. In the invention, the heating strips 41 are arranged on the inner side wall of the sliding groove 15, and when the culture dish 42 slides in the sliding groove 15 along with the objective table 14, the culture dish 42 is just heated by the heating strips 41 arranged on the inner side wall of the sliding groove 15, and the heating distance is short. In addition, the sliding groove 15 is designed in shape, only the upper outlet can effectively avoid heat loss, the temperature of the culture dish 42 can be controlled, the structure is simple, and energy is saved.
As a further aspect of the present invention, the light sources 13 are strip-shaped light tubes, and the strip-shaped light tubes are arranged at intervals along the circumferential direction of the supporting column 11. As shown in FIG. 2, the purpose of this arrangement is to allow multiple sets of culture dishes 42 to be grown, increasing the efficiency of the growth.
As a further scheme of the invention, the strip-shaped lamp tube has adjustable brightness. The purpose of this setting is in order to set up the experimental group of the different illumination intensity of multiunit in an incubator 51, observe the influence of this variable of illumination intensity to cultivating, the experiment of being convenient for.
In a further embodiment of the present invention, the supporting column 11 is disposed at the center of the incubator 51, a ventilation opening 52 is formed in the wall of the incubator 51, and a filter 53 is disposed at the ventilation opening 52. As shown in FIG. 1, the purpose of this arrangement is to facilitate ventilation of the incubator 51, and the purpose of the strainer 53 is to prevent external dust from entering the incubator 51 through the ventilation opening 52 and contaminating the culture medium in the culture dish 42, thereby affecting the growth of plant pathogenic bacteria.
The invention also provides an intelligent plant pathogenic bacteria culture method, which mainly comprises the following steps:
s1: preparing a culture medium required for culturing plant pathogenic bacteria, and placing the culture medium in a culture dish 42;
s2: inoculating the culture dish 42 in S1 with the separated plant pathogenic bacteria;
s3: placing the culture dish 42 in the S2 on the object stage 14 in the incubator 51, and adjusting the required light intensity and temperature;
s4: and taking out the culture dish 42 after culturing for 2-3 days.
Claims (11)
1. An intelligent plant pathogenic bacteria culture system is characterized in that: the device comprises a support column (11) fixedly arranged on a rack, wherein a light source (13) is arranged on the support column (11); the device comprises a support column (11), a bearing table (12) is rotatably mounted at intervals along the axial direction of the support column (11), an objective table (14) used for placing a culture dish (42) is arranged on the bearing table (12), a sliding groove (15) is vertically formed in the bearing table (12), the objective table (14) is vertically and slidably mounted on the sliding groove (15), and a first compression spring (16) is arranged between the objective table (14) and the bottom of the sliding groove (15); a dimming mechanism is arranged beside the objective table (14), the dimming mechanism comprises a reflector (21), and the reflector (21) is used for reflecting light emitted by the light source (13) to the center of the liquid level of the paper culture dish (42); the light adjusting mechanism adjusts the reflection angle of the reflector (21) according to the displacement of the objective table (14).
2. An intelligent plant pathogenic bacteria culture system according to claim 1, characterized in that: the dimming mechanism comprises a support rod (22) fixedly arranged beside the objective table (14), a rack (23) is fixedly arranged at the edge of the bottom surface of the objective table (14), the rack (23) is positioned beside the support rod (22), a first rotating shaft (24) is rotatably arranged at the lower end in the support rod (22), and a second rotating shaft (25) is rotatably arranged at the upper end; a gear (26) and a first synchronous wheel (27) are fixedly arranged on the first rotating shaft (24), and the gear (26) is meshed with the rack (23); a second synchronizing wheel (28) is fixedly arranged on the second rotating shaft (25), and the first synchronizing wheel (27) is in synchronous transmission connection with the second synchronizing wheel (28); the reflective mirror (21) is fixedly arranged on the second rotating shaft (25).
3. An intelligent plant pathogenic bacteria culture system according to claim 2, characterized in that: the reflective mirror (21) is a convex mirror.
4. An intelligent plant pathogenic bacteria culture system according to claim 1 or 3, characterized in that: a guide mechanism is arranged on the circumferential direction of the objective table (14); the guide mechanism comprises a guide plate (31) which is arranged on the bearing table (12) in a sliding manner along the radial direction of the object table (14); a fixed plate (32) fixedly arranged on the bearing table (12) is arranged on one side, away from the objective table (14), of the guide plate (31), and a second compression spring (33) is arranged between the guide plate (31) and the fixed plate (32); one side that foreplate (31) is close to objective table (14) is provided with and blocks pole (34), block pole (34) and block foreplate (31) and remove to the direction that is close to objective table (14), block the vertical slidable mounting of pole (34) on plummer (12), block pole (34) and objective table (14) fixed connection.
5. An intelligent plant pathogenic bacteria culture system according to claim 4, characterized in that: the correcting plate (31) is arc-shaped, and the periphery of the culture dish (42) is coated after the correcting plate (31) is used for correcting.
6. An intelligent plant pathogenic bacteria culture system according to claim 5, characterized in that: the correcting plate (31) is subjected to leather skinning treatment.
7. An intelligent plant pathogenic bacteria culture system according to claim 1, characterized in that: be provided with on the inside wall of sliding tray (15) heating strip (41), heating strip (41) are used for controlling the temperature of culture dish (42).
8. An intelligent plant pathogenic bacteria culture system according to claim 1, characterized in that: the light sources (13) are strip-shaped lamp tubes which are arranged at intervals along the circumferential direction of the support columns (11).
9. An intelligent plant pathogenic bacteria culture system according to claim 8, wherein: the strip-shaped lamp tube is adjustable in brightness.
10. An intelligent plant pathogenic bacteria culture system according to claim 1, characterized in that: the support column (11) is arranged at the center in the incubator (51), a ventilation opening (52) is formed in the wall of the incubator (51), and a filter screen (53) is arranged at the ventilation opening (52).
11. An intelligent culture method for plant pathogenic bacteria, which is applied to the culture system according to any one of claims 1 to 10, wherein the culture method comprises the following steps: the method mainly comprises the following steps:
s1: preparing a culture medium required for culturing plant pathogenic bacteria, and placing the culture medium in a culture dish (42);
s2: inoculating the culture dish (42) in S1 with the separated plant pathogenic bacteria;
s3: placing the culture dish (42) in the S2 on an object stage (14) in an incubator (51), and adjusting the required light intensity and temperature;
s4: and taking out the culture dish (42) after 2-3 days of culture.
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| Application Number | Priority Date | Filing Date | Title |
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| CN202111617271.8A CN114196529A (en) | 2021-12-28 | 2021-12-28 | Intelligent plant pathogenic bacterium culture system and culture method thereof |
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| CN202111617271.8A CN114196529A (en) | 2021-12-28 | 2021-12-28 | Intelligent plant pathogenic bacterium culture system and culture method thereof |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN116574596A (en) * | 2023-07-14 | 2023-08-11 | 山东中创亿丰肥料集团有限公司 | Activity inspection device and method in microorganism culture process |
| CN117143704A (en) * | 2023-09-11 | 2023-12-01 | 上海市生物医药技术研究院 | Sperm analyzer sample preprocessing device |
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2021
- 2021-12-28 CN CN202111617271.8A patent/CN114196529A/en active Pending
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN116574596A (en) * | 2023-07-14 | 2023-08-11 | 山东中创亿丰肥料集团有限公司 | Activity inspection device and method in microorganism culture process |
| CN116574596B (en) * | 2023-07-14 | 2023-09-12 | 山东中创亿丰肥料集团有限公司 | Activity inspection device and method in microorganism culture process |
| CN117143704A (en) * | 2023-09-11 | 2023-12-01 | 上海市生物医药技术研究院 | Sperm analyzer sample preprocessing device |
| CN117143704B (en) * | 2023-09-11 | 2024-02-20 | 上海市生物医药技术研究院 | Sperm analyzer sample preprocessing device |
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