CN114166817A - Method for rapidly, qualitatively and quantitatively analyzing trace chloride ions - Google Patents
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- VEXZGXHMUGYJMC-UHFFFAOYSA-M Chloride anion Chemical compound [Cl-] VEXZGXHMUGYJMC-UHFFFAOYSA-M 0.000 title claims abstract description 58
- 238000000034 method Methods 0.000 title claims abstract description 39
- 229910052709 silver Inorganic materials 0.000 claims abstract description 32
- 239000002105 nanoparticle Substances 0.000 claims abstract description 31
- BQCADISMDOOEFD-UHFFFAOYSA-N Silver Chemical compound [Ag] BQCADISMDOOEFD-UHFFFAOYSA-N 0.000 claims abstract description 30
- 239000004332 silver Substances 0.000 claims abstract description 30
- 238000001514 detection method Methods 0.000 claims abstract description 25
- 229910017053 inorganic salt Inorganic materials 0.000 claims abstract description 17
- 238000001069 Raman spectroscopy Methods 0.000 claims abstract description 15
- SQGYOTSLMSWVJD-UHFFFAOYSA-N silver(1+) nitrate Chemical compound [Ag+].[O-]N(=O)=O SQGYOTSLMSWVJD-UHFFFAOYSA-N 0.000 claims abstract description 12
- 239000000758 substrate Substances 0.000 claims abstract description 9
- 229910001961 silver nitrate Inorganic materials 0.000 claims abstract description 6
- 238000001237 Raman spectrum Methods 0.000 claims abstract description 5
- 238000004416 surface enhanced Raman spectroscopy Methods 0.000 claims abstract description 3
- 239000000243 solution Substances 0.000 claims description 28
- -1 fluorine ions Chemical class 0.000 claims description 9
- 239000012266 salt solution Substances 0.000 claims description 5
- 150000001450 anions Chemical class 0.000 claims description 4
- 229910002651 NO3 Inorganic materials 0.000 claims description 3
- 229910052731 fluorine Inorganic materials 0.000 claims description 3
- 239000011737 fluorine Substances 0.000 claims description 3
- 239000002245 particle Substances 0.000 claims description 3
- VLTRZXGMWDSKGL-UHFFFAOYSA-M perchlorate Chemical compound [O-]Cl(=O)(=O)=O VLTRZXGMWDSKGL-UHFFFAOYSA-M 0.000 claims description 3
- FOIXSVOLVBLSDH-UHFFFAOYSA-N Silver ion Chemical compound [Ag+] FOIXSVOLVBLSDH-UHFFFAOYSA-N 0.000 claims description 2
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 abstract description 13
- 238000004611 spectroscopical analysis Methods 0.000 description 19
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 6
- OSVXSBDYLRYLIG-UHFFFAOYSA-N dioxidochlorine(.) Chemical compound O=Cl=O OSVXSBDYLRYLIG-UHFFFAOYSA-N 0.000 description 6
- CPELXLSAUQHCOX-UHFFFAOYSA-M Bromide Chemical compound [Br-] CPELXLSAUQHCOX-UHFFFAOYSA-M 0.000 description 5
- 238000001179 sorption measurement Methods 0.000 description 5
- 238000004659 sterilization and disinfection Methods 0.000 description 5
- ZAMOUSCENKQFHK-UHFFFAOYSA-N Chlorine atom Chemical compound [Cl] ZAMOUSCENKQFHK-UHFFFAOYSA-N 0.000 description 4
- 239000003153 chemical reaction reagent Substances 0.000 description 4
- 239000000460 chlorine Substances 0.000 description 4
- 229910052737 gold Inorganic materials 0.000 description 4
- 239000010931 gold Substances 0.000 description 4
- 238000012544 monitoring process Methods 0.000 description 4
- 230000035945 sensitivity Effects 0.000 description 4
- 239000004155 Chlorine dioxide Substances 0.000 description 3
- 239000006227 byproduct Substances 0.000 description 3
- 229910052801 chlorine Inorganic materials 0.000 description 3
- 235000019398 chlorine dioxide Nutrition 0.000 description 3
- 239000003651 drinking water Substances 0.000 description 3
- 235000020188 drinking water Nutrition 0.000 description 3
- 239000011780 sodium chloride Substances 0.000 description 3
- XTEGARKTQYYJKE-UHFFFAOYSA-M Chlorate Chemical compound [O-]Cl(=O)=O XTEGARKTQYYJKE-UHFFFAOYSA-M 0.000 description 2
- HEDRZPFGACZZDS-UHFFFAOYSA-N Chloroform Chemical compound ClC(Cl)Cl HEDRZPFGACZZDS-UHFFFAOYSA-N 0.000 description 2
- DIZPMCHEQGEION-UHFFFAOYSA-H aluminium sulfate (anhydrous) Chemical compound [Al+3].[Al+3].[O-]S([O-])(=O)=O.[O-]S([O-])(=O)=O.[O-]S([O-])(=O)=O DIZPMCHEQGEION-UHFFFAOYSA-H 0.000 description 2
- 238000009835 boiling Methods 0.000 description 2
- 229960001701 chloroform Drugs 0.000 description 2
- 230000000694 effects Effects 0.000 description 2
- PCHJSUWPFVWCPO-UHFFFAOYSA-N gold Chemical compound [Au] PCHJSUWPFVWCPO-UHFFFAOYSA-N 0.000 description 2
- 238000010438 heat treatment Methods 0.000 description 2
- 239000011259 mixed solution Substances 0.000 description 2
- 238000002360 preparation method Methods 0.000 description 2
- 239000002994 raw material Substances 0.000 description 2
- 238000011160 research Methods 0.000 description 2
- 238000003756 stirring Methods 0.000 description 2
- FFRBMBIXVSCUFS-UHFFFAOYSA-N 2,4-dinitro-1-naphthol Chemical compound C1=CC=C2C(O)=C([N+]([O-])=O)C=C([N+]([O-])=O)C2=C1 FFRBMBIXVSCUFS-UHFFFAOYSA-N 0.000 description 1
- 206010007269 Carcinogenicity Diseases 0.000 description 1
- KZBUYRJDOAKODT-UHFFFAOYSA-N Chlorine Chemical compound ClCl KZBUYRJDOAKODT-UHFFFAOYSA-N 0.000 description 1
- DGAQECJNVWCQMB-PUAWFVPOSA-M Ilexoside XXIX Chemical compound C[C@@H]1CC[C@@]2(CC[C@@]3(C(=CC[C@H]4[C@]3(CC[C@@H]5[C@@]4(CC[C@@H](C5(C)C)OS(=O)(=O)[O-])C)C)[C@@H]2[C@]1(C)O)C)C(=O)O[C@H]6[C@@H]([C@H]([C@@H]([C@H](O6)CO)O)O)O.[Na+] DGAQECJNVWCQMB-PUAWFVPOSA-M 0.000 description 1
- FYYHWMGAXLPEAU-UHFFFAOYSA-N Magnesium Chemical compound [Mg] FYYHWMGAXLPEAU-UHFFFAOYSA-N 0.000 description 1
- 239000004793 Polystyrene Substances 0.000 description 1
- ZLMJMSJWJFRBEC-UHFFFAOYSA-N Potassium Chemical compound [K] ZLMJMSJWJFRBEC-UHFFFAOYSA-N 0.000 description 1
- 239000005708 Sodium hypochlorite Substances 0.000 description 1
- 239000002253 acid Substances 0.000 description 1
- 238000005054 agglomeration Methods 0.000 description 1
- 230000004931 aggregating effect Effects 0.000 description 1
- 230000002776 aggregation Effects 0.000 description 1
- 229910052782 aluminium Inorganic materials 0.000 description 1
- 238000004458 analytical method Methods 0.000 description 1
- 239000007864 aqueous solution Substances 0.000 description 1
- 238000001479 atomic absorption spectroscopy Methods 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 230000007670 carcinogenicity Effects 0.000 description 1
- 231100000260 carcinogenicity Toxicity 0.000 description 1
- 150000001768 cations Chemical class 0.000 description 1
- 239000003795 chemical substances by application Substances 0.000 description 1
- 229910001902 chlorine oxide Inorganic materials 0.000 description 1
- 229910001919 chlorite Inorganic materials 0.000 description 1
- 229910052619 chlorite group Inorganic materials 0.000 description 1
- FOCAUTSVDIKZOP-UHFFFAOYSA-N chloroacetic acid Chemical compound OC(=O)CCl FOCAUTSVDIKZOP-UHFFFAOYSA-N 0.000 description 1
- 229940106681 chloroacetic acid Drugs 0.000 description 1
- QBWCMBCROVPCKQ-UHFFFAOYSA-N chlorous acid Chemical compound OCl=O QBWCMBCROVPCKQ-UHFFFAOYSA-N 0.000 description 1
- 238000001816 cooling Methods 0.000 description 1
- 230000007547 defect Effects 0.000 description 1
- 239000002384 drinking water standard Substances 0.000 description 1
- 229940079593 drug Drugs 0.000 description 1
- 239000003814 drug Substances 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 230000002708 enhancing effect Effects 0.000 description 1
- 238000003891 environmental analysis Methods 0.000 description 1
- 230000007613 environmental effect Effects 0.000 description 1
- 238000002795 fluorescence method Methods 0.000 description 1
- 230000007674 genetic toxicity Effects 0.000 description 1
- 231100000025 genetic toxicology Toxicity 0.000 description 1
- 229910052736 halogen Inorganic materials 0.000 description 1
- 150000002367 halogens Chemical class 0.000 description 1
- 238000001095 inductively coupled plasma mass spectrometry Methods 0.000 description 1
- 150000002500 ions Chemical class 0.000 description 1
- 239000011777 magnesium Substances 0.000 description 1
- 229910052749 magnesium Inorganic materials 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 239000002086 nanomaterial Substances 0.000 description 1
- 239000008239 natural water Substances 0.000 description 1
- 229910052755 nonmetal Inorganic materials 0.000 description 1
- 230000005693 optoelectronics Effects 0.000 description 1
- 229920002223 polystyrene Polymers 0.000 description 1
- 239000011591 potassium Substances 0.000 description 1
- 229910052700 potassium Inorganic materials 0.000 description 1
- 238000004451 qualitative analysis Methods 0.000 description 1
- 238000004445 quantitative analysis Methods 0.000 description 1
- 239000011734 sodium Substances 0.000 description 1
- 229910052708 sodium Inorganic materials 0.000 description 1
- 239000001509 sodium citrate Substances 0.000 description 1
- SUKJFIGYRHOWBL-UHFFFAOYSA-N sodium hypochlorite Chemical compound [Na+].Cl[O-] SUKJFIGYRHOWBL-UHFFFAOYSA-N 0.000 description 1
- 230000001954 sterilising effect Effects 0.000 description 1
- 238000012360 testing method Methods 0.000 description 1
- HRXKRNGNAMMEHJ-UHFFFAOYSA-K trisodium citrate Chemical compound [Na+].[Na+].[Na+].[O-]C(=O)CC(O)(CC([O-])=O)C([O-])=O HRXKRNGNAMMEHJ-UHFFFAOYSA-K 0.000 description 1
- 229940038773 trisodium citrate Drugs 0.000 description 1
- 229910021642 ultra pure water Inorganic materials 0.000 description 1
- 239000012498 ultrapure water Substances 0.000 description 1
Images
Classifications
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
- G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
- G01N21/65—Raman scattering
- G01N21/658—Raman scattering enhancement Raman, e.g. surface plasmons
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- Health & Medical Sciences (AREA)
- Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
- Physics & Mathematics (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- General Physics & Mathematics (AREA)
- Immunology (AREA)
- Pathology (AREA)
- Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
Abstract
The invention discloses a method for rapidly detecting and analyzing trace chloride ions, which specifically comprises the following steps: (1) preparing silver nanoparticle sol by using silver nitrate solution to form an SERS enhanced substrate; (2) putting the silver nanoparticle sol prepared in the step (1) into a 96-well plate, adding a solution to be detected and containing chloride ions, and adding high-concentration inorganic salt to induce the silver nanoparticle sol to agglomerate to form a solution to be detected; (3) performing Raman spectrum detection on the solution to be detected to be positioned at 242cm‑1Taking the characteristic Raman peaks of the Ag-Cl on the left and the right as references, recording the intensity and the position of the characteristic peaks, and carrying out qualitative and quantitative detection on chloride ions in a sample to be detected; the method provided by the invention can realize qualitative and quantitative detection of trace chloride ions in the water body, and has the advantages of simplicity, convenience, rapidness, low cost, high stability and the like.
Description
Technical Field
The invention belongs to the technical field of detection methods of non-metal ions in aqueous solution, and particularly relates to a method for rapidly, qualitatively and quantitatively analyzing trace chloride ions.
Background
Chloride ions widely exist in natural water and cannot cause harm to human under normal conditions. The liquid chlorine method, the sodium hypochlorite method and the chlorine dioxide method are commonly used for disinfection in urban drinking water treatment plants, chlorinated disinfection byproducts such as chlorinated organic matters such as trichloromethane, chloroacetic acid and the like are generated in the process, and the byproducts have carcinogenicity and genetic toxicity to human bodies. The chlorate limit value is 0.7mg/L, the chloride limit value is 250mg/L, and the concentration limit values of the disinfection byproducts of the trichloromethane and the chlorite (when using chlorine dioxide for disinfection) are 0.06mg/L and 0.7mg/L respectively according to the sanitary standard of domestic drinking water issued in China (GB 5749-2006). In order to control the water quality at the tail end of the pipe network, the drinking water standard simultaneously stipulates the residual amount of water at the tail end of the pipe network, wherein the minimum values of free chlorine, total chlorine and chlorine dioxide are respectively 0.05mg/L, 0.05mg/L and 0.02 mg/L. Too high residual chlorine brings odor to water, and too low residual chlorine causes water to lose the capability of maintaining sterilization, thereby reducing the sanitary safety of water supply. Therefore, the method has important practical significance for ensuring the water quality safety by sensitively and efficiently detecting the content of chloride ions in the drinking water treatment process and at the tail end of a pipe network.
At present, common methods for detecting chloride ions include an atomic fluorescence method, an atomic absorption spectrometry method, an inductively coupled plasma mass spectrometry method and the like, but the methods have the defects of large and expensive equipment volume, long detection time, complex operation, incapability of on-line monitoring and the like, and limit the wide application of the methods. Therefore, the search for a chloride ion detection method which is simple, fast, economical, efficient and capable of on-line monitoring has become one of the important research directions in the fields of food, sanitation, environmental analysis and the like at present.
The Surface Enhanced Raman Spectroscopy (SERS) method has the advantages of simplicity and convenience in operation, low detection cost, high sensitivity, high analysis speed and the like, and has great application potential in various fields such as environmental monitoring, biological detection, food science and the like. At present, Ag and Au-based nano materials are the most commonly used SERS enhanced substrates, and a patent with the application number of CN202110123684.4 discloses a method for quickly, qualitatively and quantitatively analyzing trace bromide ions, and particularly discloses a method for preparing gold nanoparticle sol by using chloroauric acid solution to form an SERS enhanced substrate; so that the bromide ions are strongly adsorbed on the surface of the gold nanoparticle sol by forming Au-Br bonds, and qualitative and quantitative detection is carried out on the bromide ions in the sample to be detected. The bromide ions and the chloride ions are halogen elements, and can be used for qualitative and quantitative detection of the halide ions in the water body by using an SERS method, but compared with strong adsorption of the bromide ions and the iodide ions, the chloride ions are relatively weak in adsorption on the surfaces of Au and Ag of a common SERS substrate, so that the difficulty in detecting the chloride ions by using the SERS method is caused; meanwhile, some currently disclosed SERS methods for detecting chloride ions in water mainly focus on qualitative detection of chloride ions in water, lack of exploration on sensitivity detection of chloride ions, and restrict application and popularization of the SERS method for detecting trace chloride ions.
Disclosure of Invention
In order to solve the problems in the prior art, the invention provides a method for quickly, qualitatively and quantitatively analyzing trace chloride ions, which can realize convenient, quick, low-cost, high-sensitivity and high-stability detection of chloride ions in the environment.
The technical scheme of the invention is as follows:
a method for rapidly detecting and analyzing trace chloride ions specifically comprises the following steps:
(1) preparing silver nanoparticle sol by using silver nitrate solution to form an SERS enhanced substrate;
(2) putting the silver nanoparticle sol prepared in the step (1) into a 96-well plate, adding a solution to be detected and containing chloride ions, and adding high-concentration inorganic salt to induce the silver nanoparticle sol to agglomerate to form a solution to be detected;
(3) performing Raman spectrum detection on the solution to be detected to be positioned at 242cm-1And taking the left and right Ag-Cl characteristic Raman peaks as references, recording the intensities and positions of the characteristic peaks, and simultaneously constructing a linear relation between the concentration of the chloride ions and the Ag-Cl characteristic Raman peak intensity so as to qualitatively and quantitatively detect the chloride ions in the sample to be detected.
Further, the particle size of the silver nano-ions in the silver nano-particle sol in the step (1) is 15-150 nm.
Further, the high-concentration inorganic salt in the step (3) is an inorganic salt solution with a concentration of 0.1-1M and capable of weakly adsorbing anions such as fluorine ions, perchlorate ions and nitrate ions.
Further, the solution containing chloride ions and the silver nanoparticle sol are added according to the volume ratio of 10: 1; the high-concentration inorganic salt and the silver nanoparticle sol are added according to the volume ratio of 1: 1.
The invention has the beneficial effects that:
(1) the SERS method for rapidly detecting and analyzing the trace chloride ions, provided by the invention, simultaneously researches the detection sensitivity of the trace chloride ions, and the lowest detectable concentration is 10-6And M can be used for quantitative and qualitative detection of trace chloride ions in the water body.
(2) The SERS method provided by the invention has the advantages of simplicity, convenience, rapidness, low cost, high stability and the like for detecting trace chloride ions in water, and meanwhile, the portable Raman spectrometer is convenient for on-line monitoring and has potential market value.
(3) According to the SERS method for rapidly detecting and analyzing the trace chloride ions, provided by the invention, the silver nanoparticle sol prepared from the silver nitrate solution without chloride ions is used for forming the SERS enhanced substrate, and the inorganic salt with weak adsorption anions is used as an aggregating agent, so that the adsorption of the chloride ions is not interfered while a high-activity SERS hot spot is created, and the detection of the trace chloride ions is realized.
Drawings
Fig. 1 is a raman spectrum corresponding to chloride ions of different concentrations in a chloride ion detection method established in embodiment 1 of the present invention;
FIG. 2 is a graph of the intensity of the SERS signal of chloride ions as a function of concentration, as established in example 1 of the present invention.
Detailed Description
The invention will be further described with reference to the accompanying drawings and specific examples, which are given by way of illustration only and are not to be construed as limiting the invention; unless otherwise specified, the reagent raw materials used in the following examples are biochemical reagent raw materials which are conventionally commercially available or commercially available, and the laboratory instruments used are laboratory conventional instruments, and unless otherwise specified, the methods and apparatuses used in the following examples are those conventionally used in the art.
(1) Preparation of reagents: sodium chloride (analytically pure) and aluminum sulfate (analytically pure) are all produced by chemical reagents of the national drug group, ltd; polystyrene 96 well plates for detection (Corning, usa);
(2) preparing an instrument:
a raman spectrometer (budatake opto-electronics technologies, ltd);
example 1
A method for rapidly detecting and analyzing trace chloride ions specifically comprises the following steps:
(1) preparing silver nanoparticle sol by using silver nitrate solution to form an SERS enhanced substrate, wherein the particle size of silver nanoparticles in the silver nanoparticle sol is within the range of 15-150 nm;
(2) putting the silver nanoparticle sol prepared in the step (1) into a 96-well plate, adding a solution to be detected and containing chloride ions, and adding high-concentration inorganic salt to induce the silver nanoparticle sol to agglomerate to form a solution to be detected;
the working principle that chloride ions are adsorbed on the surface of the silver nanoparticle sol and high-concentration inorganic salt is added in the step (3) to promote agglomeration to finally form a solution to be detected for SERS detection is as follows:
(3) performing Raman spectrum detection on the solution to be detected to be positioned at 242cm-1Recording the intensity and position of the characteristic peak by taking the left and right Ag-Cl characteristic Raman peaks as a reference, and simultaneously constructing a linear relation between the concentration of the chloride ions and the Ag-Cl characteristic Raman peak intensity so as to qualitatively and quantitatively detect the chloride ions in the sample to be detected;
therein, at 10-7M-10-2In the range of M concentration, the linear relation curve of the chloride ion concentration and the characteristic Raman peak intensity of Ag-Cl is that y is 11.308+1.162lnx, x is the chloride ion concentration, and y is positioned at 242cm-1Characteristic raman peak intensity of Ag-Cl.
Further, the preparation method of the silver nanoparticle sol in the step (1) comprises the following steps: putting 5mL of 53mmol/L silver nitrate solution and 40mL of ultrapure water into a 100mL double-mouth round-bottom flask, continuously stirring the solution at the rotating speed of 1500r/min, heating the solution to boiling for 3-5min, quickly adding 5mL of 1% (m/v) trisodium citrate solution, changing the solution from colorless to golden yellow, finally changing the solution to grey green, keeping boiling for 30min, stopping heating, stirring and cooling the solution to room temperature to prepare silver nanoparticle sol, transferring the silver nanoparticle sol into a 50mL centrifuge tube, and storing the silver nanoparticle sol in a refrigerator at 4 ℃ for later use;
further, the high-concentration inorganic salt and the silver nanoparticle sol are added according to the volume ratio of 1: 1; adding a solution containing chloride ions and silver nanoparticle sol according to a volume ratio of 10:1, wherein the high-concentration inorganic salt is an inorganic salt solution with weak anion adsorption, such as fluorine ions, perchlorate ions and nitrate ions with the concentration of 0.1-1M
Example 2 chloride ion detection sensitivity test
First, the concentrations were 1M and 10M, respectively-1M、10-2M、10-3M、10-4M、10-5M、10-6M、10-7M、10-8M、10-9M sodium chloride solutions with different concentration gradients are shaken by hand and uniformly mixed;
secondly, preparing silver nanoparticle sol according to the method in example 1 to form an SERS enhancing substrate;
then, adding 20 mu L of silver nanoparticle sol into a 96-well plate, respectively adding 200 mu L of sodium chloride solutions with different concentrations as detection samples into the 96-well plate, and finally adding 20 mu L of aluminum sulfate solution (or other inorganic salt solution taking sodium, potassium, magnesium or aluminum ions as cations) selected as high-concentration inorganic salt into the 96-well plate to form a mixed solution to be detected;
finally, the mixed solution to be measured is placed in a Raman spectrometer to obtain a Raman spectrogram shown in figure 1, wherein the Raman spectrogram is 242cm-1Is a characteristic Raman peak of chloride ions with a lowest detectable concentration of 10-6And M, performing qualitative and quantitative analysis on the chloride ions in the sample to be detected.
Referring to the attached figure 2, it can be known that when the concentration of the chloride ions in the water body is 0.1M, the intensity of the SERS signal of the chloride ions is strongest, and the detection effect is most obvious.
The above description is only an embodiment of the present invention, and not intended to limit the scope of the present invention, and all modifications of equivalent structures and equivalent processes, which are made by the present specification, or directly or indirectly applied to other related technical fields, are included in the scope of the present invention.
Claims (4)
1. A method for rapidly detecting and analyzing trace chloride ions specifically comprises the following steps:
(1) preparing silver nanoparticle sol by using silver nitrate solution to form an SERS enhanced substrate;
(2) putting the silver nanoparticle sol prepared in the step (1) into a 96-well plate, adding a solution to be detected and containing chloride ions, and adding high-concentration inorganic salt to induce the silver nanoparticle sol to agglomerate to form a solution to be detected;
(3) performing Raman spectrum detection on the solution to be detected to be positioned at 242cm-1And taking the left and right Ag-Cl characteristic Raman peaks as references, recording the intensities and positions of the characteristic peaks, and simultaneously constructing a linear relation between the concentration of the chloride ions and the Ag-Cl characteristic Raman peak intensity so as to qualitatively and quantitatively detect the chloride ions in the sample to be detected.
2. The method for rapidly detecting and analyzing the trace chloride ions according to claim 1, wherein the method comprises the following steps: the particle size of the silver nanoparticles in the silver nanoparticle sol in the step (1) is 15-150 nm.
3. The method for rapidly detecting and analyzing the trace chloride ions according to claim 2, wherein the method comprises the following steps: the high-concentration inorganic salt in the step (3) is an inorganic salt solution with a concentration of 0.1-1M and weakly adsorbing anions such as fluorine ions, perchlorate ions and nitrate ions.
4. The method for rapidly detecting and analyzing the trace chloride ions according to claim 3, wherein the method comprises the following steps: the solution containing the chloride ions and the silver nanoparticle sol are added according to the volume ratio of 10: 1; the high-concentration inorganic salt solution and the silver nanoparticle sol are added according to the volume ratio of 1: 1.
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| CN115219479A (en) * | 2022-09-21 | 2022-10-21 | 中国科学院烟台海岸带研究所 | A kind of method for detecting Ag+ in high concentration Cl- environment |
| CN117070211A (en) * | 2022-05-09 | 2023-11-17 | 深圳大学 | Composite material, preparation method and application thereof |
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