CN114145309A - Sample treatment fluid and preparation method thereof - Google Patents
Sample treatment fluid and preparation method thereof Download PDFInfo
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- CN114145309A CN114145309A CN202111505283.1A CN202111505283A CN114145309A CN 114145309 A CN114145309 A CN 114145309A CN 202111505283 A CN202111505283 A CN 202111505283A CN 114145309 A CN114145309 A CN 114145309A
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- sample
- sodium
- digestive juice
- disinfectant
- treatment
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- 239000012530 fluid Substances 0.000 title claims abstract description 22
- 238000002360 preparation method Methods 0.000 title claims abstract description 13
- 230000001079 digestive effect Effects 0.000 claims abstract description 48
- 235000011389 fruit/vegetable juice Nutrition 0.000 claims abstract description 46
- 239000000645 desinfectant Substances 0.000 claims abstract description 35
- 239000005708 Sodium hypochlorite Substances 0.000 claims abstract description 28
- SUKJFIGYRHOWBL-UHFFFAOYSA-N sodium hypochlorite Chemical compound [Na+].Cl[O-] SUKJFIGYRHOWBL-UHFFFAOYSA-N 0.000 claims abstract description 28
- 238000001514 detection method Methods 0.000 claims abstract description 26
- 239000007788 liquid Substances 0.000 claims abstract description 26
- 239000002994 raw material Substances 0.000 claims abstract description 19
- 238000004659 sterilization and disinfection Methods 0.000 claims abstract description 7
- MSLRPWGRFCKNIZ-UHFFFAOYSA-J tetrasodium;hydrogen peroxide;dicarbonate Chemical compound [Na+].[Na+].[Na+].[Na+].OO.OO.OO.[O-]C([O-])=O.[O-]C([O-])=O MSLRPWGRFCKNIZ-UHFFFAOYSA-J 0.000 claims abstract description 7
- KWYUFKZDYYNOTN-UHFFFAOYSA-M Potassium hydroxide Chemical compound [OH-].[K+] KWYUFKZDYYNOTN-UHFFFAOYSA-M 0.000 claims description 42
- TWRXJAOTZQYOKJ-UHFFFAOYSA-L Magnesium chloride Chemical compound [Mg+2].[Cl-].[Cl-] TWRXJAOTZQYOKJ-UHFFFAOYSA-L 0.000 claims description 28
- -1 polyoxyethylene Polymers 0.000 claims description 24
- 239000006144 Dulbecco’s modified Eagle's medium Substances 0.000 claims description 22
- 239000008367 deionised water Substances 0.000 claims description 19
- 229910021641 deionized water Inorganic materials 0.000 claims description 19
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Chemical compound O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 19
- KEZYHIPQRGTUDU-UHFFFAOYSA-N 2-[dithiocarboxy(methyl)amino]acetic acid Chemical compound SC(=S)N(C)CC(O)=O KEZYHIPQRGTUDU-UHFFFAOYSA-N 0.000 claims description 14
- 102000009027 Albumins Human genes 0.000 claims description 14
- 108010088751 Albumins Proteins 0.000 claims description 14
- WJLVQTJZDCGNJN-UHFFFAOYSA-N Chlorhexidine hydrochloride Chemical compound Cl.Cl.C=1C=C(Cl)C=CC=1NC(N)=NC(N)=NCCCCCCN=C(N)N=C(N)NC1=CC=C(Cl)C=C1 WJLVQTJZDCGNJN-UHFFFAOYSA-N 0.000 claims description 14
- 102000029816 Collagenase Human genes 0.000 claims description 14
- 108060005980 Collagenase Proteins 0.000 claims description 14
- 229920003171 Poly (ethylene oxide) Polymers 0.000 claims description 14
- 102000004142 Trypsin Human genes 0.000 claims description 14
- 108090000631 Trypsin Proteins 0.000 claims description 14
- 239000003242 anti bacterial agent Substances 0.000 claims description 14
- 229960004504 chlorhexidine hydrochloride Drugs 0.000 claims description 14
- 229960002424 collagenase Drugs 0.000 claims description 14
- GVGUFUZHNYFZLC-UHFFFAOYSA-N dodecyl benzenesulfonate;sodium Chemical compound [Na].CCCCCCCCCCCCOS(=O)(=O)C1=CC=CC=C1 GVGUFUZHNYFZLC-UHFFFAOYSA-N 0.000 claims description 14
- RTZKZFJDLAIYFH-UHFFFAOYSA-N ether Substances CCOCC RTZKZFJDLAIYFH-UHFFFAOYSA-N 0.000 claims description 14
- 229910001629 magnesium chloride Inorganic materials 0.000 claims description 14
- WSFSSNUMVMOOMR-NJFSPNSNSA-N methanone Chemical compound O=[14CH2] WSFSSNUMVMOOMR-NJFSPNSNSA-N 0.000 claims description 14
- 229940080264 sodium dodecylbenzenesulfonate Drugs 0.000 claims description 14
- 239000012588 trypsin Substances 0.000 claims description 14
- 238000002156 mixing Methods 0.000 claims description 13
- 238000001125 extrusion Methods 0.000 claims description 10
- MWNQXXOSWHCCOZ-UHFFFAOYSA-L sodium;oxido carbonate Chemical compound [Na+].[O-]OC([O-])=O MWNQXXOSWHCCOZ-UHFFFAOYSA-L 0.000 claims description 7
- 230000000249 desinfective effect Effects 0.000 claims description 6
- 238000000034 method Methods 0.000 claims description 6
- 238000005070 sampling Methods 0.000 claims description 6
- 125000005037 alkyl phenyl group Chemical group 0.000 claims description 4
- 230000001954 sterilising effect Effects 0.000 abstract description 5
- 238000004043 dyeing Methods 0.000 abstract description 4
- 230000000694 effects Effects 0.000 abstract description 4
- WSFSSNUMVMOOMR-UHFFFAOYSA-N Formaldehyde Chemical compound O=C WSFSSNUMVMOOMR-UHFFFAOYSA-N 0.000 abstract 3
- BZSXEZOLBIJVQK-UHFFFAOYSA-N 2-methylsulfonylbenzoic acid Chemical compound CS(=O)(=O)C1=CC=CC=C1C(O)=O BZSXEZOLBIJVQK-UHFFFAOYSA-N 0.000 description 1
- 206010036790 Productive cough Diseases 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- VTIIJXUACCWYHX-UHFFFAOYSA-L disodium;carboxylatooxy carbonate Chemical compound [Na+].[Na+].[O-]C(=O)OOC([O-])=O VTIIJXUACCWYHX-UHFFFAOYSA-L 0.000 description 1
- 239000006185 dispersion Substances 0.000 description 1
- 244000000010 microbial pathogen Species 0.000 description 1
- 229940045872 sodium percarbonate Drugs 0.000 description 1
- 210000003802 sputum Anatomy 0.000 description 1
- 208000024794 sputum Diseases 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N47/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom not being member of a ring and having no bond to a carbon or hydrogen atom, e.g. derivatives of carbonic acid
- A01N47/40—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom not being member of a ring and having no bond to a carbon or hydrogen atom, e.g. derivatives of carbonic acid the carbon atom having a double or triple bond to nitrogen, e.g. cyanates, cyanamides
- A01N47/42—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom not being member of a ring and having no bond to a carbon or hydrogen atom, e.g. derivatives of carbonic acid the carbon atom having a double or triple bond to nitrogen, e.g. cyanates, cyanamides containing —N=CX2 groups, e.g. isothiourea
- A01N47/44—Guanidine; Derivatives thereof
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N35/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having two bonds to hetero atoms with at the most one bond to halogen, e.g. aldehyde radical
- A01N35/02—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having two bonds to hetero atoms with at the most one bond to halogen, e.g. aldehyde radical containing aliphatically bound aldehyde or keto groups, or thio analogues thereof; Derivatives thereof, e.g. acetals
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N59/00—Biocides, pest repellants or attractants, or plant growth regulators containing elements or inorganic compounds
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
- G01N1/38—Diluting, dispersing or mixing samples
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- General Health & Medical Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Agronomy & Crop Science (AREA)
- Plant Pathology (AREA)
- Engineering & Computer Science (AREA)
- Dentistry (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- Environmental Sciences (AREA)
- Physics & Mathematics (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- General Physics & Mathematics (AREA)
- Immunology (AREA)
- Pathology (AREA)
- Pest Control & Pesticides (AREA)
- Inorganic Chemistry (AREA)
- Acyclic And Carbocyclic Compounds In Medicinal Compositions (AREA)
- Agricultural Chemicals And Associated Chemicals (AREA)
- Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
Abstract
The invention belongs to the technical field of sample preparation, in particular to a sample treatment fluid and a preparation method thereof, aiming at the problems that the observation of results can be influenced because a sample pretreatment step is basically absent in some detection in the prior clinic, the direct collection of the sample has the problem of biological safety, the direct collection and the direct treatment require the personal operation of doctors, the labor and the time are wasted, and the compliance of some samples is poor, the following scheme is proposed, wherein the scheme comprises a disinfectant and a digestive juice, and the disinfectant comprises the following raw materials in percentage by weight: 2-4% of sodium hypochlorite, 2-3% of formaldehyde and 1-2% of sodium carbonate peroxide, the sample treatment liquid has the sterilization effect of over 95%, the early treatment has no obvious influence on the further operation of late dyeing and the like, the non-contact sample addition can be realized through the storage device, the biological infectivity is reduced, the operation is simple, convenient and quick, and the manual operation is omitted.
Description
Technical Field
The invention relates to the technical field of sample preparation, in particular to a sample treatment fluid and a preparation method thereof.
Background
Samples such as sputum, alveolar lavage fluid and the like have the biological safety problem, and the sampled partial samples are viscous, so that the distribution density of pathogenic microorganisms in the samples is not uniform, and the later detection result is inaccurate;
at present, in some clinical tests, a sample pretreatment step is basically not needed, the observation of results can be influenced, samples are directly collected, the biological safety problem exists in direct treatment, the direct collection and direct treatment needs the personal operation of doctors, the labor and the time are wasted, and the compliance of some samples is poor.
Disclosure of Invention
The invention aims to solve the problems that in the prior art, certain detection in clinic basically has no sample pretreatment step, which may affect the observation of results, the direct collection of samples and the biological safety problem of the direct treatment exist, the direct collection and the direct treatment require the personal operation of doctors, the labor and the time are wasted, and the compliance of some samples is poor, and provides a sample treatment solution and a preparation method thereof.
The sample treatment fluid provided by the invention comprises a disinfectant and a digestive juice, wherein the disinfectant comprises the following raw materials in percentage by weight: 2-4% of sodium hypochlorite, 2-3% of formaldehyde, 1-2% of sodium peroxycarbonate, 0.1-0.5% of antibacterial agent, 1-3% of polyoxyethylene alkyl phenyl ether, 5-10% of chlorhexidine hydrochloride, 5-8% of sodium dodecyl benzene sulfonate, 1-3% of sodium metasilicate pentahydrate and the balance of deionized water, wherein the digestive juice comprises the following raw materials in percentage by weight: sodium hypochlorite 1-1.5%, potassium hydroxide 2-3%, trypsin 8-12%, collagenase 0.5-0.8%, magnesium chloride 0.5-1%, albumin 3-5%, and the balance DMEM culture solution.
Preferably, the disinfectant comprises the following raw materials in percentage by weight: 3-5% of sodium hypochlorite, 3-4% of formaldehyde, 2-4% of sodium carbonate peroxide, 0.2-0.6% of antibacterial agent, 2-4% of polyoxyethylene alkyl phenyl ether, 7-11% of chlorhexidine hydrochloride, 6-10% of sodium dodecyl benzene sulfonate, 2-4% of sodium metasilicate pentahydrate and the balance of deionized water, wherein the digestive juice comprises the following raw materials in percentage by weight: 2-3% of sodium hypochlorite, 3-4% of potassium hydroxide, 10-13% of trypsin, 0.8-1.3% of collagenase, 1-1.5% of magnesium chloride, 4-6% of albumin and the balance of DMEM culture solution.
Preferably, the disinfectant comprises the following raw materials in percentage by weight: 3.5% of sodium hypochlorite, 3% of formaldehyde, 2.5% of sodium carbonate peroxide, 0.3% of antibacterial agent, 2.5% of polyoxyethylene alkylphenyl ether, 10% of chlorhexidine hydrochloride, 9% of sodium dodecyl benzene sulfonate, 3.5% of sodium metasilicate pentahydrate and the balance of deionized water, wherein the digestive juice comprises the following raw materials in percentage by weight: 2.8% of sodium hypochlorite, 3.7% of potassium hydroxide, 11% of trypsin, 1.2% of collagenase, 1% of magnesium chloride, 5.5% of albumin and the balance of DMEM culture solution.
Preferably, the preparation method comprises the following steps:
s1: dissolving sodium hypochlorite, formaldehyde, sodium peroxycarbonate, an antibacterial agent, polyoxyethylene alkylphenyl ether, chlorhexidine hydrochloride, sodium dodecyl benzene sulfonate and sodium metasilicate pentahydrate in deionized water in sequence, and uniformly mixing to obtain a disinfectant;
s2: dissolving digestive juice including sodium hypochlorite, potassium hydroxide, trypsin, collagenase, magnesium chloride and albumin in a DMEM culture solution in sequence, and uniformly mixing to obtain the digestive juice;
and S3, slowly dissolving the prepared disinfectant into the digestive juice to prepare a treatment solution, directly filling the treatment solution into a sterile storage system, directly opening a valve for treatment when in use, and then dripping the treated sample by extrusion so as to carry out the next detection.
Preferably, in S1, the concentration of the obtained disinfecting solution is detected by a concentration detecting instrument, and deionized water is gradually added, so that the disinfecting solution with low concentration is finally obtained by reading on the concentration detecting instrument.
Preferably, in S2, the concentration of the obtained digestive juice is detected by a concentration detector, and the final digestive juice is obtained by reading on the concentration detector by adding DMEM culture solution step by step.
Preferably, in S3, the ratio of the disinfectant to the digestive juice may be 1:1, 2:3 or 3: 2.
Preferably, aseptic storage system includes the sample collection pipe, be equipped with the valve on the sample collection pipe, the valve will gather the pipe and divide into two parts from top to bottom, the upper portion is used for splendid attire treatment fluid, the lower part is the air traffic, be used for the sampling, patient or doctor adopt can open the valve according to the time requirement after gathering the sample in the air traffic for during treatment fluid flows into the sample, be equipped with the drip that can the dropping liquid through the extrusion on the top of valve, this drip is the state sealed by the lid before dropping the appearance, can disinfect and disinfection treatment to the sample after treatment fluid adds the sample, liquid after the processing is the even liquid of dispersion, so that next step's experimental operation.
The invention has the beneficial effects that:
the sample treatment fluid has the advantages that the sterilization effect can reach more than 95%, the sterilization time is 15-30 minutes, the pretreatment has no obvious influence on the further operation of later-stage dyeing and the like, the valve is adjusted after sampling, the treatment fluid can be automatically added into a sample, the operation is simple, convenient and quick, manual operation is omitted, the storage device can realize non-contact sample addition, the biological infectivity is reduced, the automatic film making and reading in the later stage are easy, the operation of the device is simple and convenient, the device is not limited by people, time and conditions, the operation process of adding the treatment fluid by a patient can be realized, the valve can be opened at any time with set time, the upper-layer treatment fluid flows into the sample, the device reports that the process of adding the treatment fluid is pollution-free, the time and the times of contact of the sample with people and the air are reduced.
The sample treatment liquid has the sterilization effect of over 95 percent, the early treatment has no obvious influence on the further operation of later dyeing and the like, the non-contact sample addition can be realized through the storage device, the biological infectivity is reduced, the operation is simple, convenient and quick, and the manual operation is saved.
Detailed Description
The present invention will be further illustrated with reference to the following specific examples.
Example one
The invention provides a sample treatment fluid, which comprises a disinfectant and a digestive juice, wherein the disinfectant comprises the following raw materials in percentage by weight: 2% of sodium hypochlorite, 2% of formaldehyde, 1% of sodium carbonate peroxide, 0.2% of an antibacterial agent, 1.5% of polyoxyethylene alkylphenyl ether, 6% of chlorhexidine hydrochloride, 5% of sodium dodecyl benzene sulfonate, 1% of sodium metasilicate pentahydrate and the balance of deionized water, wherein the digestive liquid comprises the following raw materials in percentage by weight: 1% of sodium chlorate, 2% of potassium hydroxide, 9% of trypsin, 0.5% of collagenase, 0.5% of magnesium chloride, 3.3% of albumin and the balance of DMEM culture solution.
The preparation method comprises the following steps:
s1: sequentially dissolving sodium hypochlorite, formaldehyde, sodium peroxycarbonate, an antibacterial agent, polyoxyethylene alkylphenyl ether, chlorhexidine hydrochloride, sodium dodecyl benzene sulfonate and sodium metasilicate pentahydrate in deionized water, uniformly mixing to obtain a disinfectant, performing concentration detection on the obtained disinfectant by using a concentration detection instrument, gradually adding deionized water, and finally obtaining a low-concentration disinfectant by reading on the concentration detection instrument;
s2: sequentially dissolving digestive juice including sodium hypochlorite, potassium hydroxide, trypsin, collagenase, magnesium chloride and albumin in a DMEM culture solution, uniformly mixing the digestive juice and the DMEM culture solution to obtain a digestive juice, carrying out concentration detection on the obtained digestive juice by a concentration detection instrument, gradually adding the DMEM culture solution, and obtaining a final digestive juice by reading on the concentration detection instrument;
s3, slowly dissolving the prepared disinfectant into the digestive juice to prepare a treatment solution, directly filling the treatment solution into a sterile storage system, directly opening a valve to treat the treatment solution when in use, and dripping the treated sample by extrusion to perform next detection, wherein the mixing ratio of the disinfectant to the digestive juice can be 1:1, the sterile storage system comprises a sample collection pipe, the sample collection pipe is provided with a valve, the collection pipe is divided into an upper part and a lower part by the valve, the upper part is used for containing the treatment solution, the lower part is an empty pipe for sampling, the valve can be opened according to time requirements after a patient or a doctor collects the sample in the empty pipe to enable the treatment solution to flow into the sample, the top end of the valve is provided with a dropping opening which can be used for dropping liquid by extrusion, the dropping opening is sealed by a cover before dropping the sample, and the treatment solution can be sterilized and disinfected after being added into the sample, the treated liquid is a uniformly dispersed liquid for the next experimental operation.
Example two
The invention provides a sample treatment fluid, which comprises a disinfectant and a digestive juice, wherein the disinfectant comprises the following raw materials in percentage by weight: 4% of sodium hypochlorite, 3.5% of formaldehyde, 3% of sodium percarbonate, 0.4% of antibacterial agent, 3% of polyoxyethylene alkylphenyl ether, 9% of chlorhexidine hydrochloride, 8% of sodium dodecyl benzene sulfonate, 3% of sodium metasilicate pentahydrate and the balance of deionized water, wherein the digestive juice comprises the following raw materials in percentage by weight: 2.5% of sodium hypochlorite, 3.5% of potassium hydroxide, 12% of trypsin, 1% of collagenase, 1.3% of magnesium chloride, 5% of albumin and the balance of DMEM culture solution.
The preparation method comprises the following steps:
s1: sequentially dissolving sodium hypochlorite, formaldehyde, sodium peroxycarbonate, an antibacterial agent, polyoxyethylene alkylphenyl ether, chlorhexidine hydrochloride, sodium dodecyl benzene sulfonate and sodium metasilicate pentahydrate in deionized water, uniformly mixing to obtain a disinfectant, performing concentration detection on the obtained disinfectant by using a concentration detection instrument, gradually adding deionized water, and finally obtaining a low-concentration disinfectant by reading on the concentration detection instrument;
s2: sequentially dissolving digestive juice including sodium hypochlorite, potassium hydroxide, trypsin, collagenase, magnesium chloride and albumin in a DMEM culture solution, uniformly mixing the digestive juice and the DMEM culture solution to obtain a digestive juice, carrying out concentration detection on the obtained digestive juice by a concentration detection instrument, gradually adding the DMEM culture solution, and obtaining a final digestive juice by reading on the concentration detection instrument;
s3, slowly dissolving the prepared disinfectant into the digestive juice to prepare a treatment solution, directly filling the treatment solution into a sterile storage system, directly opening a valve to treat the treatment solution when in use, and dripping the treated sample by extrusion to perform next detection, wherein the mixing ratio of the disinfectant to the digestive juice can be 2:3, the sterile storage system comprises a sample collection pipe, the sample collection pipe is provided with a valve, the collection pipe is divided into an upper part and a lower part by the valve, the upper part is used for containing the treatment solution, the lower part is an empty pipe for sampling, the valve can be opened according to time requirements after a patient or a doctor collects the sample in the empty pipe to enable the treatment solution to flow into the sample, the top end of the valve is provided with a dropping opening which can be used for dropping liquid by extrusion, the dropping opening is sealed by a cover before dropping the sample, and the treatment solution can be sterilized and disinfected after being added into the sample, the treated liquid is a uniformly dispersed liquid for the next experimental operation.
EXAMPLE III
The invention provides a sample treatment fluid, which comprises a disinfectant and a digestive juice, wherein the disinfectant comprises the following raw materials in percentage by weight: 3.5% of sodium hypochlorite, 3% of formaldehyde, 2.5% of sodium carbonate peroxide, 0.3% of antibacterial agent, 2.5% of polyoxyethylene alkylphenyl ether, 10% of chlorhexidine hydrochloride, 9% of sodium dodecyl benzene sulfonate, 3.5% of sodium metasilicate pentahydrate and the balance of deionized water, wherein the digestive juice comprises the following raw materials in percentage by weight: 2.8% of sodium hypochlorite, 3.7% of potassium hydroxide, 11% of trypsin, 1.2% of collagenase, 1% of magnesium chloride, 5.5% of albumin and the balance of DMEM culture solution.
The preparation method comprises the following steps:
s1: sequentially dissolving sodium hypochlorite, formaldehyde, sodium peroxycarbonate, an antibacterial agent, polyoxyethylene alkylphenyl ether, chlorhexidine hydrochloride, sodium dodecyl benzene sulfonate and sodium metasilicate pentahydrate in deionized water, uniformly mixing to obtain a disinfectant, performing concentration detection on the obtained disinfectant by using a concentration detection instrument, gradually adding deionized water, and finally obtaining a low-concentration disinfectant by reading on the concentration detection instrument;
s2: sequentially dissolving digestive juice including sodium hypochlorite, potassium hydroxide, trypsin, collagenase, magnesium chloride and albumin in a DMEM culture solution, uniformly mixing the digestive juice and the DMEM culture solution to obtain a digestive juice, carrying out concentration detection on the obtained digestive juice by a concentration detection instrument, gradually adding the DMEM culture solution, and obtaining a final digestive juice by reading on the concentration detection instrument;
s3, slowly dissolving the prepared disinfectant into the digestive juice to prepare a treatment solution, directly filling the treatment solution into a sterile storage system, directly opening a valve to treat the treatment solution when in use, and dripping the treated sample by extrusion to perform next detection, wherein the mixing ratio of the disinfectant to the digestive juice can be 3:2, the sterile storage system comprises a sample collection pipe, the sample collection pipe is provided with a valve, the collection pipe is divided into an upper part and a lower part by the valve, the upper part is used for containing the treatment solution, the lower part is an empty pipe for sampling, the valve can be opened according to time requirements after a patient or a doctor collects the sample in the empty pipe to enable the treatment solution to flow into the sample, the top end of the valve is provided with a dropping opening which can be used for dropping liquid by extrusion, the dropping opening is sealed by a cover before dropping the sample, and the treatment solution can be sterilized and disinfected after being added into the sample, the treated liquid is a uniformly dispersed liquid for the next experimental operation.
Through the sample treatment liquid and the preparation method thereof provided by the first, second and third embodiments, the sterilization effect of the sample treatment liquid can reach more than 95%, the early treatment has no obvious influence on the further operations such as late dyeing and the like, the non-contact sample addition can be realized through the storage device, the bio-infectivity is reduced, the operation is simple, convenient and quick, the manual operation is omitted, and the second embodiment is the best embodiment.
The above description is only for the preferred embodiment of the present invention, but the scope of the present invention is not limited thereto, and any person skilled in the art should be considered to be within the technical scope of the present invention, and the technical solutions and the inventive concepts thereof according to the present invention should be equivalent or changed within the scope of the present invention.
Claims (8)
1. The sample processing liquid is characterized by comprising a disinfectant and a digestive liquid, wherein the disinfectant comprises the following raw materials in percentage by weight: 2-4% of sodium hypochlorite, 2-3% of formaldehyde, 1-2% of sodium peroxycarbonate, 0.1-0.5% of antibacterial agent, 1-3% of polyoxyethylene alkyl phenyl ether, 5-10% of chlorhexidine hydrochloride, 5-8% of sodium dodecyl benzene sulfonate, 1-3% of sodium metasilicate pentahydrate and the balance of deionized water, wherein the digestive juice comprises the following raw materials in percentage by weight: sodium hypochlorite 1-1.5%, potassium hydroxide 2-3%, trypsin 8-12%, collagenase 0.5-0.8%, magnesium chloride 0.5-1%, albumin 3-5%, and the balance DMEM culture solution.
2. The sample processing fluid of claim 1, wherein the disinfecting fluid comprises the following raw materials by weight percent: 3-5% of sodium hypochlorite, 3-4% of formaldehyde, 2-4% of sodium carbonate peroxide, 0.2-0.6% of antibacterial agent, 2-4% of polyoxyethylene alkyl phenyl ether, 7-11% of chlorhexidine hydrochloride, 6-10% of sodium dodecyl benzene sulfonate, 2-4% of sodium metasilicate pentahydrate and the balance of deionized water, wherein the digestive juice comprises the following raw materials in percentage by weight: 2-3% of sodium hypochlorite, 3-4% of potassium hydroxide, 10-13% of trypsin, 0.8-1.3% of collagenase, 1-1.5% of magnesium chloride, 4-6% of albumin and the balance of DMEM culture solution.
3. The sample processing fluid of claim 1, wherein the disinfecting fluid comprises the following raw materials by weight percent: 3.5% of sodium hypochlorite, 3% of formaldehyde, 2.5% of sodium carbonate peroxide, 0.3% of antibacterial agent, 2.5% of polyoxyethylene alkylphenyl ether, 10% of chlorhexidine hydrochloride, 9% of sodium dodecyl benzene sulfonate, 3.5% of sodium metasilicate pentahydrate and the balance of deionized water, wherein the digestive juice comprises the following raw materials in percentage by weight: 2.8% of sodium hypochlorite, 3.7% of potassium hydroxide, 11% of trypsin, 1.2% of collagenase, 1% of magnesium chloride, 5.5% of albumin and the balance of DMEM culture solution.
4. A preparation method of a sample treatment fluid is characterized by comprising the following steps:
s1: dissolving sodium hypochlorite, formaldehyde, sodium peroxycarbonate, an antibacterial agent, polyoxyethylene alkylphenyl ether, chlorhexidine hydrochloride, sodium dodecyl benzene sulfonate and sodium metasilicate pentahydrate in deionized water in sequence, and uniformly mixing to obtain a disinfectant;
s2: dissolving digestive juice including sodium hypochlorite, potassium hydroxide, trypsin, collagenase, magnesium chloride and albumin in a DMEM culture solution in sequence, and uniformly mixing to obtain the digestive juice;
and S3, slowly dissolving the prepared disinfectant into the digestive juice to prepare a treatment solution, directly filling the treatment solution into a sterile storage system, directly opening a valve for treatment when in use, and then dripping the treated sample by extrusion so as to carry out the next detection.
5. The method as claimed in claim 4, wherein in step S1, the concentration of the disinfecting solution obtained is detected by a concentration detector, and deionized water is gradually added, so that the disinfecting solution with low concentration is finally obtained by reading on the concentration detector.
6. The method for preparing a sample processing solution according to claim 4, wherein in the step S2, the concentration of the obtained digestive juice is detected by a concentration detector, and the final digestive juice is obtained by reading the concentration detector with the DMEM culture solution added step by step.
7. The method of claim 4, wherein in step S3, the disinfectant solution and the digestive juice are mixed at a ratio of 1:1, 2:3 or 3: 2.
8. A method for preparing a specimen processing liquid according to claim 4, wherein the sterile storage system comprises a specimen collection tube, a valve is arranged on the specimen collection tube, the collection tube is divided into an upper part and a lower part by the valve, the upper part is used for containing the processing liquid, the lower part is an empty tube and is used for sampling, the valve can be opened according to time requirements after a patient or a doctor collects a specimen in the empty tube, the processing liquid flows into the specimen, a dropping opening capable of dropping liquid through extrusion is arranged at the top end of the valve, the dropping opening is in a state of being sealed by a cover before dropping the sample, the processing liquid is added into the specimen and then is subjected to sterilization and disinfection treatment, and the processed liquid is uniformly dispersed liquid so as to facilitate the next experiment operation.
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Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN203539362U (en) * | 2013-11-13 | 2014-04-16 | 桂林医学院附属医院 | Disposable collecting container of peripheral blood chromosome blood specimen |
| CN102676452B (en) * | 2012-04-26 | 2014-09-24 | 天津美德太平洋科技有限公司 | Culture medium containing human umbilical cord mesenchymal stem cell exudates and preparation method and applications thereof |
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102676452B (en) * | 2012-04-26 | 2014-09-24 | 天津美德太平洋科技有限公司 | Culture medium containing human umbilical cord mesenchymal stem cell exudates and preparation method and applications thereof |
| CN203539362U (en) * | 2013-11-13 | 2014-04-16 | 桂林医学院附属医院 | Disposable collecting container of peripheral blood chromosome blood specimen |
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