CN114145305B - Composition for preventing and treating citrus moss disease - Google Patents
Composition for preventing and treating citrus moss disease Download PDFInfo
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- CN114145305B CN114145305B CN202111601259.8A CN202111601259A CN114145305B CN 114145305 B CN114145305 B CN 114145305B CN 202111601259 A CN202111601259 A CN 202111601259A CN 114145305 B CN114145305 B CN 114145305B
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- 241000207199 Citrus Species 0.000 title claims abstract description 45
- 235000020971 citrus fruits Nutrition 0.000 title claims abstract description 45
- 239000000203 mixture Substances 0.000 title claims abstract description 20
- 201000010099 disease Diseases 0.000 title abstract description 33
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 title abstract description 33
- YBHILYKTIRIUTE-UHFFFAOYSA-N berberine Chemical compound C1=C2CC[N+]3=CC4=C(OC)C(OC)=CC=C4C=C3C2=CC2=C1OCO2 YBHILYKTIRIUTE-UHFFFAOYSA-N 0.000 claims abstract description 36
- 229940093265 berberine Drugs 0.000 claims abstract description 36
- QISXPYZVZJBNDM-UHFFFAOYSA-N berberine Natural products COc1ccc2C=C3N(Cc2c1OC)C=Cc4cc5OCOc5cc34 QISXPYZVZJBNDM-UHFFFAOYSA-N 0.000 claims abstract description 36
- WSWCOQWTEOXDQX-MQQKCMAXSA-M (E,E)-sorbate Chemical compound C\C=C\C=C\C([O-])=O WSWCOQWTEOXDQX-MQQKCMAXSA-M 0.000 claims abstract description 15
- 239000000575 pesticide Substances 0.000 claims abstract description 13
- 229940075554 sorbate Drugs 0.000 claims abstract description 13
- 208000019756 lichen disease Diseases 0.000 claims abstract description 11
- 235000010241 potassium sorbate Nutrition 0.000 claims description 18
- 239000004302 potassium sorbate Substances 0.000 claims description 18
- 229940069338 potassium sorbate Drugs 0.000 claims description 18
- CHHHXKFHOYLYRE-UHFFFAOYSA-M 2,4-Hexadienoic acid, potassium salt (1:1), (2E,4E)- Chemical group [K+].CC=CC=CC([O-])=O CHHHXKFHOYLYRE-UHFFFAOYSA-M 0.000 claims description 16
- 238000002360 preparation method Methods 0.000 claims description 9
- 239000007864 aqueous solution Substances 0.000 claims description 4
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- 239000004563 wettable powder Substances 0.000 claims description 3
- 239000004283 Sodium sorbate Substances 0.000 claims description 2
- MCFVRESNTICQSJ-RJNTXXOISA-L calcium sorbate Chemical compound [Ca+2].C\C=C\C=C\C([O-])=O.C\C=C\C=C\C([O-])=O MCFVRESNTICQSJ-RJNTXXOISA-L 0.000 claims description 2
- 235000010244 calcium sorbate Nutrition 0.000 claims description 2
- 239000004303 calcium sorbate Substances 0.000 claims description 2
- LROWVYNUWKVTCU-STWYSWDKSA-M sodium sorbate Chemical compound [Na+].C\C=C\C=C\C([O-])=O LROWVYNUWKVTCU-STWYSWDKSA-M 0.000 claims description 2
- 235000019250 sodium sorbate Nutrition 0.000 claims description 2
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- 239000003795 chemical substances by application Substances 0.000 description 6
- 229940079593 drug Drugs 0.000 description 6
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- 241000196324 Embryophyta Species 0.000 description 5
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 5
- BEFDCLMNVWHSGT-UHFFFAOYSA-N ethenylcyclopentane Chemical compound C=CC1CCCC1 BEFDCLMNVWHSGT-UHFFFAOYSA-N 0.000 description 5
- 150000001875 compounds Chemical class 0.000 description 4
- WSWCOQWTEOXDQX-UHFFFAOYSA-N 2,4-Hexadienoic acid Chemical compound CC=CC=CC(O)=O WSWCOQWTEOXDQX-UHFFFAOYSA-N 0.000 description 3
- OKKJLVBELUTLKV-UHFFFAOYSA-N Methanol Chemical compound OC OKKJLVBELUTLKV-UHFFFAOYSA-N 0.000 description 3
- 230000003902 lesion Effects 0.000 description 3
- -1 polyoxyethylene Polymers 0.000 description 3
- 239000013589 supplement Substances 0.000 description 3
- QTBSBXVTEAMEQO-UHFFFAOYSA-N Acetic acid Chemical compound CC(O)=O QTBSBXVTEAMEQO-UHFFFAOYSA-N 0.000 description 2
- HEDRZPFGACZZDS-UHFFFAOYSA-N Chloroform Chemical compound ClC(Cl)Cl HEDRZPFGACZZDS-UHFFFAOYSA-N 0.000 description 2
- 241000195628 Chlorophyta Species 0.000 description 2
- 241000195493 Cryptophyta Species 0.000 description 2
- RTZKZFJDLAIYFH-UHFFFAOYSA-N Diethyl ether Chemical compound CCOCC RTZKZFJDLAIYFH-UHFFFAOYSA-N 0.000 description 2
- 206010059866 Drug resistance Diseases 0.000 description 2
- 102000004190 Enzymes Human genes 0.000 description 2
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- 238000013329 compounding Methods 0.000 description 2
- 239000013078 crystal Substances 0.000 description 2
- JHIVVAPYMSGYDF-UHFFFAOYSA-N cyclohexanone Chemical compound O=C1CCCCC1 JHIVVAPYMSGYDF-UHFFFAOYSA-N 0.000 description 2
- 239000008367 deionised water Substances 0.000 description 2
- 229910021641 deionized water Inorganic materials 0.000 description 2
- 239000002552 dosage form Substances 0.000 description 2
- 235000013305 food Nutrition 0.000 description 2
- 239000005452 food preservative Substances 0.000 description 2
- 238000002844 melting Methods 0.000 description 2
- 230000008018 melting Effects 0.000 description 2
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- 229940068196 placebo Drugs 0.000 description 2
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- VZGDMQKNWNREIO-UHFFFAOYSA-N tetrachloromethane Chemical compound ClC(Cl)(Cl)Cl VZGDMQKNWNREIO-UHFFFAOYSA-N 0.000 description 2
- IXPNQXFRVYWDDI-UHFFFAOYSA-N 1-methyl-2,4-dioxo-1,3-diazinane-5-carboximidamide Chemical compound CN1CC(C(N)=N)C(=O)NC1=O IXPNQXFRVYWDDI-UHFFFAOYSA-N 0.000 description 1
- 239000005995 Aluminium silicate Substances 0.000 description 1
- 244000144730 Amygdalus persica Species 0.000 description 1
- 241000832615 Apatococcus lobatus Species 0.000 description 1
- 241000894006 Bacteria Species 0.000 description 1
- 241000133570 Berberidaceae Species 0.000 description 1
- 235000004936 Bromus mango Nutrition 0.000 description 1
- 241001672694 Citrus reticulata Species 0.000 description 1
- 241001529387 Colletotrichum gloeosporioides Species 0.000 description 1
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- 235000016887 Leptospermum scoparium Nutrition 0.000 description 1
- 240000007228 Mangifera indica Species 0.000 description 1
- 235000014826 Mangifera indica Nutrition 0.000 description 1
- 235000002637 Nicotiana tabacum Nutrition 0.000 description 1
- 244000061176 Nicotiana tabacum Species 0.000 description 1
- 241000223665 Phaeococcus Species 0.000 description 1
- 229920003171 Poly (ethylene oxide) Polymers 0.000 description 1
- 235000006040 Prunus persica var persica Nutrition 0.000 description 1
- VYPSYNLAJGMNEJ-UHFFFAOYSA-N Silicium dioxide Chemical compound O=[Si]=O VYPSYNLAJGMNEJ-UHFFFAOYSA-N 0.000 description 1
- DBMJMQXJHONAFJ-UHFFFAOYSA-M Sodium laurylsulphate Chemical compound [Na+].CCCCCCCCCCCCOS([O-])(=O)=O DBMJMQXJHONAFJ-UHFFFAOYSA-M 0.000 description 1
- 235000004489 Sorbus commixta Nutrition 0.000 description 1
- 241001115347 Sorbus commixta Species 0.000 description 1
- 235000009184 Spondias indica Nutrition 0.000 description 1
- 229960000583 acetic acid Drugs 0.000 description 1
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- 229930013930 alkaloid Natural products 0.000 description 1
- 150000003797 alkaloid derivatives Chemical group 0.000 description 1
- 235000012211 aluminium silicate Nutrition 0.000 description 1
- 230000001580 bacterial effect Effects 0.000 description 1
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- 230000008859 change Effects 0.000 description 1
- RQFQJYYMBWVMQG-IXDPLRRUSA-N chitotriose Chemical compound O[C@@H]1[C@@H](N)[C@H](O)O[C@H](CO)[C@H]1O[C@H]1[C@H](N)[C@@H](O)[C@H](O[C@H]2[C@@H]([C@@H](O)[C@H](O)[C@@H](CO)O2)N)[C@@H](CO)O1 RQFQJYYMBWVMQG-IXDPLRRUSA-N 0.000 description 1
- 238000004040 coloring Methods 0.000 description 1
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- 230000003111 delayed effect Effects 0.000 description 1
- 239000003085 diluting agent Substances 0.000 description 1
- GVGUFUZHNYFZLC-UHFFFAOYSA-N dodecyl benzenesulfonate;sodium Chemical compound [Na].CCCCCCCCCCCCOS(=O)(=O)C1=CC=CC=C1 GVGUFUZHNYFZLC-UHFFFAOYSA-N 0.000 description 1
- 238000003912 environmental pollution Methods 0.000 description 1
- 239000000945 filler Substances 0.000 description 1
- 235000019249 food preservative Nutrition 0.000 description 1
- 230000002538 fungal effect Effects 0.000 description 1
- 230000000855 fungicidal effect Effects 0.000 description 1
- 239000000417 fungicide Substances 0.000 description 1
- 239000012362 glacial acetic acid Substances 0.000 description 1
- 150000004676 glycans Chemical class 0.000 description 1
- 230000036541 health Effects 0.000 description 1
- 230000001788 irregular Effects 0.000 description 1
- NLYAJNPCOHFWQQ-UHFFFAOYSA-N kaolin Chemical compound O.O.O=[Al]O[Si](=O)O[Si](=O)O[Al]=O NLYAJNPCOHFWQQ-UHFFFAOYSA-N 0.000 description 1
- 230000002045 lasting effect Effects 0.000 description 1
- 239000007788 liquid Substances 0.000 description 1
- 231100000053 low toxicity Toxicity 0.000 description 1
- 238000000034 method Methods 0.000 description 1
- 230000000813 microbial effect Effects 0.000 description 1
- 210000001700 mitochondrial membrane Anatomy 0.000 description 1
- 231100000252 nontoxic Toxicity 0.000 description 1
- 230000003000 nontoxic effect Effects 0.000 description 1
- 230000000361 pesticidal effect Effects 0.000 description 1
- 229930015704 phenylpropanoid Natural products 0.000 description 1
- 150000002995 phenylpropanoid derivatives Chemical class 0.000 description 1
- 229940068918 polyethylene glycol 400 Drugs 0.000 description 1
- 229920001282 polysaccharide Polymers 0.000 description 1
- 239000005017 polysaccharide Substances 0.000 description 1
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- 238000012827 research and development Methods 0.000 description 1
- 150000003839 salts Chemical class 0.000 description 1
- 235000010413 sodium alginate Nutrition 0.000 description 1
- 239000000661 sodium alginate Substances 0.000 description 1
- 229940005550 sodium alginate Drugs 0.000 description 1
- 229940080264 sodium dodecylbenzenesulfonate Drugs 0.000 description 1
- 239000002904 solvent Substances 0.000 description 1
- 230000009044 synergistic interaction Effects 0.000 description 1
- 125000003396 thiol group Chemical group [H]S* 0.000 description 1
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Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N43/00—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
- A01N43/90—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having two or more relevant hetero rings, condensed among themselves or with a common carbocyclic ring system
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N37/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids
- A01N37/06—Unsaturated carboxylic acids or thio analogues thereof; Derivatives thereof
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- Life Sciences & Earth Sciences (AREA)
- Agronomy & Crop Science (AREA)
- Pest Control & Pesticides (AREA)
- Plant Pathology (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Dentistry (AREA)
- General Health & Medical Sciences (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- Environmental Sciences (AREA)
- Agricultural Chemicals And Associated Chemicals (AREA)
Abstract
The invention belongs to the technical field of pesticides, and particularly relates to a composition for preventing and treating citrus moss. The composition for preventing and treating citrus moss disease comprises the effective components of berberine and sorbate in a mass ratio of 1:19-19:1. the berberine and the sorbate in the composition have obvious synergistic effect on citrus lichen disease germs, and can improve the control effect on the citrus lichen disease germs.
Description
Technical Field
The invention belongs to the technical field of pesticides, and particularly relates to a composition for preventing and treating citrus moss.
Background
Citrus is planted in 140 countries and regions around the world as a globally important bulk fruit, and the global citrus planting area is further increased to 681.7 million hectares in 2019. China is one of the original producing areas of citrus, the planting area and the yield are continuously increased in recent years, the number of the planted citrus is increased to 247.3 million hectares in 2019, the citrus is an important characteristic crop for promoting the income increase of farmers in rural areas and mountain areas in south China, and the citrus is the most important industry for assisting the vogue of rural areas. . In general, the growth of citrus is facilitated in high-temperature rainy and warm humid climates, but the growth of citrus is also particularly easy to cause disease damage, in rainy seasons, particularly in citrus orchards with poor ventilation, a layer of green clothes is often covered on trunks, branches and leaves, even the ground, which is called green moss disease, and the pathogen is pseudopluvialis (Apatococcus lobatus) of pseudoplutella of the family Phaeodaceae of the phylum Chlorophyta, and colletotrichum gloeosporioides can infect citrus together with algae to cause the green moss disease. The citrus leaves and branches and fruits are easy to get ill under the high-temperature and high-humidity environment. The planting area of Guangxi is more than 800 ten thousand mu, and the annual occurrence area is about 8 ten thousand mu. When the citrus leaves are infected, yellow-green small points appear at the midvein, the apex and the edge of the front face of the citrus leaves, and then gradually expand towards the periphery to form irregular plaques which are mutually healed to cover the whole leaves, and the back faces of the leaves are almost asymptomatic; when the branches are infected with diseases, yellow-green small spots appear on the surfaces of the branches first, and then green patches are gradually formed by expanding to cover the whole trunk and the surfaces of the branches; when the fruits are affected by diseases, the fruits are infected with the diseases and the disease spots appear from the color change, often appear from the fruit base and gradually spread to other parts, and when the fruits are seriously affected, the whole fruit surface is covered with green stains, so that the coloring and the quality of the fruits are influenced.
Berberine is also called berberine, is a phenylpropanoid quaternary alkaloid with molecular formula C 20 H 18 NO 4 It is a yellow needle crystal, with melting point of 145 deg.C, and is soluble in water and insoluble in benzene, ether and chloroform. It is contained in many plants of berberidaceae and the like. Due to the broad-spectrum antibacterial effect, the bacterial cell has higher control effect on citrus lichen disease through destroying the integrity of cell walls, cytoplasmic membranes and mitochondrial membranes and destroying DNA to cause fungal cell death. However, if berberine is singly applied for a long time to prevent and treat the citrus lichen diseases, the berberine is easy to causePathogenic bacteria generate drug resistance, so that the control effect of the berberine is reduced.
Sorbic acid common name: sorbus commixta acid and Leptospermum scoparium acid with chemical name of 2, 4-hexadienoic acid or 2-propenyl acrylic acid and molecular formula of C 6 H 8 O 2 The sorbic acid has a molecular weight of 112, is a high-efficiency and safe preservative recommended by the international food and agriculture organization and the health organization, and is widely applied to the industries of food, beverage, tobacco, pesticide, cosmetics and the like. Sorbic acid is colorless needle crystal or white crystalline powder, has melting point of 130-135 deg.C and boiling point of 228 deg.C, has no odor or slight pungent odor, and is stable to light and heat; slightly soluble in water, easily soluble in methanol, ethanol, glacial acetic acid, and acetone, soluble in acetone, benzene, and carbon tetrachloride, sour, and nontoxic. Sorbic acid and its salts can bind to sulfhydryl groups in microbial enzymes, destroy the action of the enzymes and cause retardation of reproduction.
The compounding of different chemical pesticides is a common method for research and development of new pesticides, and research of the inventor finds that the control effect of the citrus greening disease can be remarkably improved when berberine and sorbate are compounded according to a certain mass ratio, and related reports are not found at present.
The information disclosed in this background section is only for enhancement of understanding of the general background of the invention and should not be taken as an acknowledgement or any form of suggestion that this information forms the prior art already known to a person skilled in the art.
Disclosure of Invention
The invention aims to provide a composition for preventing and treating citrus moss, which can generate a synergistic effect when the active ingredients of berberine and sorbate are compounded, so that the generation of drug resistance of pathogenic bacteria can be delayed, and the prevention and treatment effects on the pathogenic bacteria can be improved.
In order to realize the purpose, the invention provides the following technical scheme:
the invention aims to provide a composition for preventing and treating citrus lichen disease, which comprises the effective components of berberine and sorbate in a mass ratio of 1:19-19:1.
preferably, the mass ratio of the berberine to the sorbate is 1:9-9:1.
preferably, the mass ratio of the berberine to the sorbate is 1:3.
preferably, the sorbate salt is potassium sorbate, sodium sorbate or calcium sorbate.
The second purpose of the invention is to provide a pesticide preparation containing the composition for preventing and treating the moss diseases, wherein the mass of the berberine and the sorbate accounts for 0.2-80% of the total mass of the pesticide preparation, and the balance is an agriculturally and pharmaceutically acceptable auxiliary component. The agriculturally and pharmaceutically acceptable auxiliary components include auxiliaries, solvents, fillers and the like, which are known to the person skilled in the art.
Preferably, the pesticide preparation is in the form of wettable powder, aqueous solution or aqueous emulsion.
The third purpose of the invention is to provide the application of the composition in preventing and treating citrus lichen disease.
Compared with the prior art, the invention has the following beneficial effects:
(1) The berberine and the sorbate in the composition for preventing and treating the moss disease have obvious synergistic interaction effect on citrus moss disease germs and can improve the prevention and treatment effect on the citrus moss disease germs.
(2) The berberine in the composition for preventing and treating the moss disease is a botanical fungicide, so that the composition has small environmental pollution; the potassium sorbate is a food preservative and preservative agent, has low toxicity, has good safety after being compounded, and meets the safety requirement of pesticide preparations.
Detailed Description
In the following, the technical solutions of the present invention are described clearly and completely, and it is obvious that the described embodiments are some, but not all, embodiments of the present invention. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.
Examples1
0.5g berberine, 1.5g potassium sorbate, 2.0g peach gum polysaccharide, 3.0g ethanol, 2.0g polyethylene glycol 400, 5.0g glycerol, adding deionized water to supplement to 100g, and making into 2% berberine potassium sorbate aqua.
Examples2
3.0g berberine, 7.0g potassium sorbate, 1.0g chitosan oligosaccharide, 10g cyclohexanone, 3g alkylphenol polyoxyethylene, 4.5g sodium dodecyl benzene sulfonate, 5.0g glycerol, adding deionized water to supplement to 100g, and making into 10% berberine and sorbic acid water emulsion.
Examples3
20.0g of berberine, 60.0g of potassium sorbate, 3.0g of sodium dodecyl sulfate, 5.0g of sodium alginate and 5.0g of white carbon black, and kaolin is added to supplement the mixture to 100g to prepare 80 percent berberine-sorbic acid wettable powder.
The field drug effect1：Berberine and potassium sorbate compounded aqueous solution has effect of preventing and treating citrus lichen disease
The test method refers to the pesticide field efficacy test criterion (II) GB/T17980.98-2004: bactericide for preventing and treating mango anthracnose
In a Nanfeng mandarin orange orchard of Daliantown of Weian county, shanghai, guangxi Liuzhou city, citrus trees with consistent growth vigor and plant depression and common occurrence of citrus greening disease are selected, and the pathogen is algae of Phaeococcus of Chlorophyta after identification.
The trial set up 9 treatments, 3 replicates each, with 2 fruit trees each. Each treatment is diluted by berberine and potassium sorbate compound solutions with different mass ratios, so that the mass concentration of effective components in each treatment mixed diluent is 25mg/L, and clear water is used as a blank reference. Spraying the whole plant of each treated citrus tree by adopting a spraying method in the occurrence period of the moss disease until the front and back surfaces of branches and leaves of citrus are uniformly sprayed without dripping liquid medicine.
The test was performed 7 months in 2020, with a total of 3 disease surveys: the 1 st survey is the first pre-application survey (carried out on day 8/7), and the common morbidity of the plants in the residential area is surveyed; the 2 nd survey was 7d after the first application (conducted on day 7/month and 14); the 3 rd study was 14d after the first administration (7 months and 21 days). Each tree is investigated for 5 directions in the south, the east and the north, 10 leaves are investigated for each direction, the total leaf number and the number of all levels of diseased leaves are recorded and investigated, and the disease index and the prevention and treatment effect of each treatment are calculated, and the results are shown in the following table 1.
The grading criteria of leaf disease are as follows:
stage 0: no disease spots on the leaves;
level 1: the area of the scab on the leaf is below 10%;
and 3, level: the area of the scab on the leaf is 11-25%;
stage 5: the lesion area on the leaves accounts for 26-50%;
and 7, stage: the lesion area on the leaves accounts for 51-75%;
and 9, stage: the lesion area on the leaves accounts for more than 75 percent.
The calculation formula of the disease index and the prevention and treatment effect is as follows:
in the formula: CK (CK) 0 -pre-drug disease index in placebo zone; PT 0 -pre-dose disease index in the agent treatment area; CK (CK) 1 -the disease index after administration of the placebo zone; PT 1 -post-dose disease index in the agent treatment area.
TABLE 1 prevention and treatment of Citrus Chaga disease by berberine and potassium sorbate treatment at different ratios
Note: different upper and lower case letters indicate significance levels of difference of up to 0.01 and 0.05, respectively.
As can be seen from the table 1, after the application of the drug for 7 days, the berberine and the potassium sorbate with different proportions gradually show a certain control effect on the citrus lichen diseases, and the control effect after the application of the drug for 14 days is better than that after the application of the drug for 7 days.
When the medicine is applied for 14d, the control effects of the single berberine and the single potassium sorbate are lower than 56.85 percent, and after the compound treatment is adopted, the control effects after the compound proportion of the berberine and the potassium sorbate is lower than 9 are higher than 57.16 percent and higher than the control effects of the single berberine and the single potassium sorbate; the compounding ratio of the berberine and the potassium sorbate is lower than 3, and the control effect is obviously higher than that of single agent treatment. Particularly, when the mass ratio of the berberine to the potassium sorbate is 1.
The field drug effect2：Berberine and potassium sorbate compound preparation with different concentrations and dosage forms has effect of preventing and treating citrus lichen disease
The test method refers to the field efficacy 1, the test is carried out in 2021 and 9 months, and 3 disease surveys are carried out: the 1 st survey is the first pre-application survey (carried out on 8 days 9 months), and the common morbidity of the plants in the residential area is surveyed; the 2 nd survey was 14d after the first application (conducted on day 21/9); the 3 rd study was 21d after the first application (carried out on day 28/9), and the results of the test are shown in Table 2 below.
TABLE 2 Berberine and Potassium sorbate combination preparations with different concentrations and dosage forms have effects of preventing and treating Citrus Chaga disease
Note: different upper and lower case letters indicate significance levels of difference of up to 0.01 and 0.05, respectively.
As can be seen from table 2, under the condition of using the same content of the active ingredients (namely the same mass concentration of 100 mg/L), the control effect of different compound preparations on the citrus greening disease is 75.49-75.78% of the control effect of the application agent 14d, and the control effect of the application agent 21d is 78.03-79.24%. Meanwhile, the control effect on the citrus greening disease is gradually increased along with the increase of the content of the effective components (50-200 mg/L).
Under the condition of the same mass concentration of the pesticide application of 14d and 21d, the control effect of the compound agent on the citrus greening disease is obviously higher than that of a 0.5 percent berberine aqueous solution, and the control effect shows obvious synergistic effect and better continuous control effect.
In conclusion, the composition for preventing and treating the citrus moss shows a remarkable synergistic effect on the citrus moss, can improve the prevention and treatment effect on the citrus moss, and has a lasting pesticide effect.
Claims (6)
1. A composition for preventing and treating citrus lichen disease is characterized in that the effective components are compounded by berberine and sorbate,
the mass ratio of the berberine to the sorbate is 1:9-9:1.
2. the composition according to claim 1, wherein the weight ratio of berberine to sorbate is 1:3.
3. the composition of claim 1, wherein the sorbate salt is potassium sorbate, sodium sorbate, or calcium sorbate.
4. A pesticide formulation comprising a composition according to any one of claims 1 to 3, wherein the mass of berberine and sorbate represent 0.2 to 80% of the total mass of the pesticide formulation, the remainder being agriculturally and pharmaceutically acceptable auxiliary components.
5. The pesticide preparation as claimed in claim 4, wherein the pesticide preparation is in the form of wettable powder, aqueous solution or aqueous emulsion.
6. Use of a composition according to any one of claims 1 to 3 for the control of citrus lichen disease.
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| WO2015051116A1 (en) * | 2013-10-03 | 2015-04-09 | Dow Global Technologies Llc | Microbicidal composition comprising a benzoate or sorbate salt |
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Effective date of registration: 20231025 Address after: Building 1121, Building 3, Dongdu International Plaza, No. 88 Gongye North Road, Licheng District, Jinan City, Shandong Province, 250100 Patentee after: Shandong Jiaqi Biotechnology Co.,Ltd. Address before: No. 174, Daxue East Road, XiXiangTang District, Nanning City, Guangxi Zhuang Autonomous Region Patentee before: GUANGXI ACADEMY OF AGRICULTURAL SCIENCES |