CN114137811A - A common optical path off-axis digital holographic microscope based on optical wedge - Google Patents
A common optical path off-axis digital holographic microscope based on optical wedge Download PDFInfo
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- CN114137811A CN114137811A CN202111392858.3A CN202111392858A CN114137811A CN 114137811 A CN114137811 A CN 114137811A CN 202111392858 A CN202111392858 A CN 202111392858A CN 114137811 A CN114137811 A CN 114137811A
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- 230000003287 optical effect Effects 0.000 title claims abstract description 60
- 239000000428 dust Substances 0.000 claims abstract description 33
- 239000004065 semiconductor Substances 0.000 claims abstract description 25
- 230000000903 blocking effect Effects 0.000 claims abstract description 3
- 230000007246 mechanism Effects 0.000 claims description 70
- 238000013016 damping Methods 0.000 claims description 26
- 230000010287 polarization Effects 0.000 claims description 21
- 238000003384 imaging method Methods 0.000 claims description 15
- 238000009647 digital holographic microscopy Methods 0.000 claims description 11
- 238000006243 chemical reaction Methods 0.000 claims description 4
- 125000006850 spacer group Chemical group 0.000 description 6
- 238000001228 spectrum Methods 0.000 description 6
- 238000010008 shearing Methods 0.000 description 5
- 238000000034 method Methods 0.000 description 3
- 230000009471 action Effects 0.000 description 2
- 230000007547 defect Effects 0.000 description 2
- 238000010586 diagram Methods 0.000 description 2
- 230000000694 effects Effects 0.000 description 2
- 238000001093 holography Methods 0.000 description 2
- 230000009466 transformation Effects 0.000 description 2
- 230000008859 change Effects 0.000 description 1
- 238000002474 experimental method Methods 0.000 description 1
- 230000002349 favourable effect Effects 0.000 description 1
- 238000009434 installation Methods 0.000 description 1
- 238000013508 migration Methods 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 230000002265 prevention Effects 0.000 description 1
- 230000008569 process Effects 0.000 description 1
- 230000035945 sensitivity Effects 0.000 description 1
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- G—PHYSICS
- G03—PHOTOGRAPHY; CINEMATOGRAPHY; ANALOGOUS TECHNIQUES USING WAVES OTHER THAN OPTICAL WAVES; ELECTROGRAPHY; HOLOGRAPHY
- G03H—HOLOGRAPHIC PROCESSES OR APPARATUS
- G03H1/00—Holographic processes or apparatus using light, infrared or ultraviolet waves for obtaining holograms or for obtaining an image from them; Details peculiar thereto
- G03H1/0005—Adaptation of holography to specific applications
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B08—CLEANING
- B08B—CLEANING IN GENERAL; PREVENTION OF FOULING IN GENERAL
- B08B5/00—Cleaning by methods involving the use of air flow or gas flow
- B08B5/02—Cleaning by the force of jets, e.g. blowing-out cavities
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- G—PHYSICS
- G02—OPTICS
- G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
- G02B21/00—Microscopes
- G02B21/06—Means for illuminating specimens
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- G—PHYSICS
- G02—OPTICS
- G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
- G02B21/00—Microscopes
- G02B21/24—Base structure
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- G—PHYSICS
- G02—OPTICS
- G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
- G02B21/00—Microscopes
- G02B21/24—Base structure
- G02B21/248—Base structure objective (or ocular) turrets
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- G—PHYSICS
- G02—OPTICS
- G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
- G02B21/00—Microscopes
- G02B21/24—Base structure
- G02B21/26—Stages; Adjusting means therefor
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- G—PHYSICS
- G03—PHOTOGRAPHY; CINEMATOGRAPHY; ANALOGOUS TECHNIQUES USING WAVES OTHER THAN OPTICAL WAVES; ELECTROGRAPHY; HOLOGRAPHY
- G03H—HOLOGRAPHIC PROCESSES OR APPARATUS
- G03H1/00—Holographic processes or apparatus using light, infrared or ultraviolet waves for obtaining holograms or for obtaining an image from them; Details peculiar thereto
- G03H1/0005—Adaptation of holography to specific applications
- G03H2001/005—Adaptation of holography to specific applications in microscopy, e.g. digital holographic microscope [DHM]
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Abstract
本发明涉及一种数字全息显微装置，尤其涉及一种基于光楔的共光路离轴数字全息显微装置。本发明提供一种可在保持较小的剪切量的同时获得更大的离轴干涉角的基于光楔的共光路离轴数字全息显微装置。一种基于光楔的共光路离轴数字全息显微装置，包括有第一支撑架、第二支撑架、防尘罩和半导体激光光源等，第一支撑架后侧连接有第二支撑架，第二支撑架上部设有用于阻挡灰尘的防尘罩，第一支撑架下侧设有三个弹性夹具，弹性夹具之间卡接有半导体激光光源。本发明通过调节光楔的角度，利用光楔底面反射光与斜面反射光的折射光之间会形成一定的夹角，这样可以在保持较小的剪切量的同时获得更大的离轴干涉角。
The invention relates to a digital holographic microscope device, in particular to a common optical path off-axis digital holographic microscope device based on an optical wedge. The invention provides an off-axis digital holographic microscope device based on an optical wedge, which can obtain a larger off-axis interference angle while maintaining a small amount of shear. An off-axis digital holographic microscope device based on an optical wedge with a common optical path, comprising a first support frame, a second support frame, a dust cover, a semiconductor laser light source, etc., and a second support frame is connected to the rear side of the first support frame, The upper part of the second support frame is provided with a dust cover for blocking dust, the lower side of the first support frame is provided with three elastic clamps, and a semiconductor laser light source is clamped between the elastic clamps. By adjusting the angle of the optical wedge, the invention utilizes that a certain angle is formed between the reflected light from the bottom surface of the optical wedge and the refracted light from the inclined surface, so that a larger off-axis interference can be obtained while maintaining a small shear amount. horn.
Description
Technical Field
The invention relates to a digital holographic microscope device, in particular to a common-path off-axis digital holographic microscope device based on an optical wedge.
Background
At present, a digital holographic microscope device can be mainly divided into a coaxial common-path interference system and a non-common-path off-axis interference system, but the independent coaxial common-path interference system easily influences the imaging quality of the system, the non-common-path off-axis interference system is easily disturbed by any mechanical vibration or air, the phase sensitivity is low, and the installation and adjustment of an optical path are complicated. The existing digital holographic microscope device comprises a first support frame, a second support frame, a semiconductor laser light source, a converter, a microscope objective group, a first lens, an optical wedge and a damping rotating shaft, and the device cannot obtain a larger off-axis interference angle while keeping a smaller shearing amount.
Thus, a wedge-based, common-path, off-axis, digital holographic microscopy apparatus is provided that can achieve greater off-axis interference angles while maintaining a smaller amount of shear.
Disclosure of Invention
Aiming at the defects of the prior art, the invention provides the common-path off-axis digital holographic microscope device based on the optical wedge, which can obtain a larger off-axis interference angle while keeping a smaller shearing amount, so as to overcome the defect that the existing device can not obtain a larger off-axis interference angle while keeping a smaller shearing amount.
In order to achieve the above purpose, the invention is realized by the following scheme: a common-path off-axis digital holographic microscope device based on an optical wedge comprises a first support frame, elastic clamps, a fixed rod, a connecting frame, a guide frame, a second support frame, a dust cover, a semiconductor laser light source, a beam expanding collimator, a converter, a microscope objective group, a first lens, an optical wedge, a damping rotating shaft, a second lens, a filter, a third lens, an imaging detector, a fourth lens, a non-polarization beam splitter prism, a clamping mechanism and a switching mechanism, wherein the rear side of the first support frame is connected with the second support frame, the lower side of the first support frame is connected with three elastic clamps, the semiconductor laser light source is clamped between the elastic clamps, the top of the first support frame is connected with the connecting frame, the converter is connected between the front lower side of the connecting frame and the rear upper side of the first support frame, the top of the converter is rotatably connected with the microscope objective group, and the microscope objective group consists of three objective lenses with different multiples, the middle part of the connecting frame is rotatably connected with a first lens, the upper part of the connecting frame is rotatably connected with two damping rotating shafts, an optical wedge is connected between the inner sides of the two damping rotating shafts, the upper part of the second supporting frame is provided with a dust cover for blocking dust, the upper part of the semiconductor laser light source is provided with a beam expanding collimator, one side of the upper part of the connecting frame, which is close to the optical wedge, is rotatably connected with a second lens, one side of the upper part of the connecting frame, which is close to the second lens, is rotatably connected with a filter, one side of the upper part of the connecting frame, which is close to the filter, is rotatably connected with a third lens, the front side of the upper part of the connecting frame is connected with an imaging detector, the lower side of the first supporting frame is connected with two fixed rods, a fourth lens is slidably connected between the front sides of the two fixed rods, the lower side of the connecting frame is connected with two guide frames, a non-polarization splitting prism is slidably connected between the rear sides of the two guide frames, and a clamping mechanism for clamping a sample is arranged between the first supporting frame and the second supporting frame, a switching mechanism used for driving the semiconductor laser light source to rotate is arranged between the first support frame and the second support frame, and the switching mechanism part is connected with the fourth lens.
In a preferred embodiment of the invention, the clamping mechanism comprises a first contact switch, a first electric push rod, a sliding frame, arc-shaped clamps and first springs, the first contact switch is arranged on the left front side of the second support frame, the first electric push rod is arranged on the rear lower side of the first support frame, the sliding frame is connected with the right lower side of the first support frame in a sliding manner, the right rear side of the sliding frame is connected with the right side of the first electric push rod, two arc-shaped clamps for clamping a sample are connected on the sliding frame in a sliding manner, the first springs are symmetrically arranged on the front and back of the left and right sides of the middle part of the sliding frame, and the outer sides of the four first springs are connected with the arc-shaped clamps on the same side.
In a preferred embodiment of the present invention, the switching mechanism includes a second contact switch, a gear motor, a rotating frame, a second electric push rod and a third electric push rod, the second contact switch is disposed on the front left side of the second support frame, the gear motor is connected to the upper left portion of the first support frame, the rotating frame is rotatably connected to the upper left side of the first support frame, the lower left side of the rotating frame is connected to an output shaft of the gear motor, the second electric push rod is disposed inside the rotating frame, a telescopic rod of the second electric push rod is connected to the semiconductor laser light source, the third electric push rod is connected to the lower rear side of the first support frame, and the front side of the telescopic rod of the third electric push rod is connected to the right side of the fourth lens.
In a preferred embodiment of the invention, the device further comprises a stable reference mechanism for fixing the sample, the stable reference mechanism comprises a positioning frame, second springs, wedge blocks, a clamping frame and a clamping plate, the tops of the two arc-shaped clamps are connected with the positioning frame for fixing the sample in a sliding manner, the two second springs are connected between the two positioning frames and the arc-shaped clamps on the same side, the wedge blocks are connected to the outer sides of the upper parts of the two positioning frames, the clamping frames are connected to the bottoms of the two arc-shaped clamps, the two clamping plates are connected to the front lower sides of the converter, and the wedge blocks can contact with the clamping plates when moving leftwards.
In a preferred embodiment of the present invention, the present invention further comprises a pushing mechanism for driving the non-polarizing beam splitter prism to move, the pushing mechanism comprises a fixed frame, a first photoelectric sensor and a fourth electric push rod, the fixed frame is connected to the left rear side of the upper portion of the connecting frame, the first photoelectric sensor is arranged on the left upper side of the fixed frame, the fourth electric push rod is connected to the right rear side of the upper portion of the connecting frame, and the front side of the telescopic rod of the fourth electric push rod is connected to the right side of the non-polarizing beam splitter prism.
In a preferred embodiment of the present invention, the damping device further comprises an anti-deviation mechanism for fixing the damping rotating shaft, the anti-deviation mechanism comprises fifth electric push rods, rubber clamping blocks and distance sensors, the fifth electric push rods are connected to the left and right sides of the middle portion of the connecting frame, the rubber clamping blocks for fixing the damping rotating shaft are arranged at the bottoms of the telescopic rods of the two fifth electric push rods, and the distance sensors are arranged inside the arc-shaped clamp at the front side.
In a preferred embodiment of the present invention, the damping device further comprises a three-point locking mechanism for assisting the rubber clamping block to fix the damping rotating shaft, the three-point locking mechanism comprises a pressure sensor, a fixing plate, a sixth electric push rod and a pushing block, the pressure sensor is arranged on the right side of the right rubber clamping block, the fixing plates are symmetrically arranged on the upper sides of the two fifth electric push rods in the front-back direction, the sixth electric push rod is arranged on the lower sides of the four fixing plates, and the pushing block for pushing the rubber clamping block is arranged on the inner sides of the telescopic rods of the four sixth electric push rods.
In a preferred embodiment of the present invention, the optical wedge dust remover further comprises a dust removing mechanism for removing dust from the optical wedge, wherein the dust removing mechanism comprises a second photoelectric sensor, a fan, an air deflector and a torsion spring, the second photoelectric sensor is arranged at the rear side inside the right rubber clamping block, the fan is connected to the middle of the connecting frame, the air deflector is rotatably connected to the front lower side of the fan, and the torsion spring is connected between the left side and the right side of the front side of the air deflector and the fan.
In a preferred embodiment of the invention, the optical wedge-based common-path off-axis digital holographic microscope device further comprises a control box, the control box is arranged on the left front side of the second support frame, the control box comprises a storage battery, a power supply module and a control module, the storage battery supplies power to the whole optical wedge-based common-path off-axis digital holographic microscope device, the output end of the storage battery is electrically connected with the power supply module, the power supply module is connected with a power supply main switch through a circuit, and the power supply module is electrically connected with the control module; the control module is connected with a DS1302 clock circuit and a 24C02 circuit; pressure sensor, first photoelectric sensor, second photoelectric sensor, distance sensor, first contact switch and second contact switch all pass through electric connection with control module, and gear motor passes through direct current motor with control module and just reverses the module and be connected, and fan, first electric putter, second electric putter, third electric putter, fourth electric putter, fifth electric putter and sixth electric putter all pass through relay control module with control module and are connected.
The invention has the following advantages: 1. according to the invention, by adjusting the angle of the optical wedge, a certain included angle is formed between the light reflected by the bottom surface of the optical wedge and the refracted light reflected by the inclined surface of the optical wedge, so that a larger off-axis interference angle can be obtained while a smaller shearing amount is kept.
2. The clamping plate is arranged on the positioning frame, the positioning frame is arranged on the positioning frame, and the clamping plate is arranged on the positioning frame.
3. According to the invention, the non-polarization beam splitter prism is driven to move by the telescopic rod of the fourth electric push rod, so that the non-polarization beam splitter prism does not need to be manually controlled by people to move.
4. The damping rotating shaft is limited by the rubber clamping block, so that the optical wedge is prevented from shaking when the device is operated to further influence the refraction angle of a light source, and the rubber clamping block is extruded by the push block under the action of the sixth electric push rod to further lock the damping rotating shaft by the rubber clamping block, so that the fixing effect is better.
5. The invention enables the air deflector to rotate under the action of the wind power of the fan, thereby leading the air deflector to guide the wind, and removing the dust on the optical wedge by the wind, thus avoiding the dust from influencing the normal work of the optical wedge.
Drawings
Fig. 1 is a schematic perspective view of a first perspective structure according to the present invention.
Fig. 2 is a perspective view of a second perspective structure according to the present invention.
Fig. 3 is a perspective view of a third perspective structure according to the present invention.
Fig. 4 is a partial perspective view of the present invention.
Fig. 5 is a schematic perspective view of the first support frame, the elastic clamp and the fixing rod according to the present invention.
Fig. 6 is a schematic perspective view of the connecting frame and the guiding frame of the present invention.
Fig. 7 is a schematic perspective view of the first structure without the second supporting frame and the dust cover according to the present invention.
Fig. 8 is a schematic perspective view of a second embodiment of the present invention without the second support frame and the dust cover.
Fig. 9 is a schematic perspective view of a third embodiment of the present invention with the second supporting frame and the dust cover removed.
Fig. 10 is a perspective view of a first part of the clamping mechanism of the present invention.
Fig. 11 is a perspective view of a second part of the clamping mechanism of the present invention.
Fig. 12 is a perspective view of a third part of the clamping mechanism of the present invention.
Fig. 13 is a schematic perspective view of a first portion of the switching mechanism of the present invention.
Fig. 14 is a perspective view of a second portion of the switching mechanism of the present invention.
FIG. 15 is a perspective view of a first portion of a stabilized reference mechanism according to the present invention.
FIG. 16 is a perspective view of a second portion of the stabilized reference mechanism of the invention.
Fig. 17 is a schematic perspective view of the pushing mechanism of the present invention.
Fig. 18 is a perspective view of a first part of the anti-shift mechanism of the present invention.
Fig. 19 is a perspective view of a second part of the anti-migration mechanism of the present invention.
Fig. 20 is a schematic perspective view of the three-point locking mechanism of the present invention.
Fig. 21 is a schematic perspective view of the dust removing mechanism of the present invention.
Fig. 22 is a schematic perspective view of a part of the dust removing mechanism of the present invention.
FIG. 23 is a block circuit diagram of the present invention.
Fig. 24 is a schematic circuit diagram of the present invention.
Wherein the figures include the following reference numerals: 1_ first support frame, 101_ elastic clamp, 102_ fixed bar, 103_ link frame, 104_ guide frame, 2_ second support frame, 3_ dust cover, 4_ semiconductor laser source, 5_ beam expanding collimator, 6_ converter, 7_ micro objective lens group, 8_ first lens, 9_ optical wedge, 10_ damped rotating shaft, 11_ second lens, 12_ filter, 13_ third lens, 14_ imaging detector, 15_ fourth lens, 16_ non-polarized beam splitting prism, 17_ clamping mechanism, 171_ first contact switch, 172_ control box, 173_ first electric push rod, 174_ sliding frame, 175_ arc clamp, 176_ first spring, 18_ converting mechanism 181_ second contact switch, 182_ deceleration motor, 183_ rotating frame, 184_ second electric push rod, 185_ third electric push rod, 19_ stable reference mechanism, 191_ positioning frame, 192_ second spring, 193_ wedge block, 194_ clamping frame, 195_ clamping plate, 20_ pushing mechanism, 201_ fixing frame, 202_ first photoelectric sensor, 203_ fourth electric push rod, 21_ deviation prevention mechanism, 211_ fifth electric push rod, 212_ rubber clamping block, 213_ distance sensor, 22_ three-point locking mechanism, 221_ pressure sensor, 222_ fixing plate, 223_ sixth electric push rod, 224_ pushing block, 23_ dust removal mechanism, 231_ second photoelectric sensor, 232_ fan, 233_ air deflector and 234_ torsion spring.
Detailed Description
The invention will be further described with reference to the accompanying drawings and the detailed description below:
example 1
Referring to fig. 1-14, a common-path off-axis digital holographic microscopy device based on optical wedges comprises a first support frame 1, elastic clamps 101, a fixing rod 102, a connecting frame 103, a guide frame 104, a second support frame 2, a dust cover 3, a semiconductor laser source 4, a beam expanding collimator 5, a converter 6, a microscope objective group 7, a first lens 8, an optical wedge 9, a damping rotating shaft 10, a second lens 11, a filter 12, a third lens 13, an imaging detector 14, a fourth lens 15, a non-polarization beam splitting prism 16, a clamping mechanism 17 and a switching mechanism 18, wherein the second support frame 2 is welded on the rear side of the first support frame 1, the dust cover 3 is arranged on the upper portion of the second support frame 2, three elastic clamps 101 are fixedly connected to the lower side of the first support frame 1 through bolts, a semiconductor laser source 4 is clamped between the elastic clamps 101, the beam expanding collimator 5 is arranged on the upper portion of the semiconductor laser source 4, the top of the first support frame 1 is welded with a connecting frame 103, a converter 6 is connected between the front lower side of the connecting frame 103 and the rear upper side of the first support frame 1, the top of the converter 6 is rotatably connected with a microscope objective group 7, the microscope objective group 7 consists of three objective lenses with different multiples, the middle part of the connecting frame 103 is connected with a first lens 8 through a bearing, the upper part of the connecting frame 103 is rotatably connected with two damping rotating shafts 10, an optical wedge 9 is connected between the inner sides of the two damping rotating shafts 10, the optical wedge 9 is positioned on the upper side of the first lens 8, the upper part of the connecting frame 103 is connected with a second lens 11 through a bearing, the second lens 11 is positioned on the front side of the optical wedge 9, the upper part of the connecting frame 103 is connected with a filter 12 through a bearing, the filter 12 is positioned on the front side of the second lens 11, the upper part of the connecting frame 103 is connected with a third lens 13 through a bearing, the third lens 13 is positioned on the front side of the filter 12, the front side of the upper part of the connecting frame 103 is connected with an imaging detector 14 through a bearing, the imaging detector 14 is located on the front side of the third lens 13, two fixing rods 102 are welded on the lower side of the first support frame 1, a fourth lens 15 is connected between the front sides of the two fixing rods 102 in a sliding mode, the fourth lens 15 is located on the upper side of the beam expanding collimator 5, two guide frames 104 are welded on the lower side of the connecting frame 103, a non-polarization beam splitter prism 16 is connected between the rear sides of the two guide frames 104 in a sliding mode, a clamping mechanism 17 is arranged between the first support frame 1 and the second support frame 2, a switching mechanism 18 is arranged between the first support frame 1 and the second support frame 2, and the switching mechanism 18 is connected with the fourth lens 15.
Referring to fig. 1, 10, 11 and 12, the clamping mechanism 17 includes a first contact switch 171, a first electric push rod 173, a sliding frame 174, an arc-shaped clamp 175 and a first spring 176, the first contact switch 171 is disposed on the front left side of the second support frame 2, the first electric push rod 173 is disposed on the lower rear side of the first support frame 1, the sliding frame 174 is slidably connected to the lower right side of the first support frame 1, the rear right side of the sliding frame 174 is connected to the right side of the first electric push rod 173, the two arc-shaped clamps 175 are slidably connected to the sliding frame 174, the first springs 176 are symmetrically disposed on the left and right sides of the middle portion of the sliding frame 174, and the outer sides of the four first springs 176 are connected to the arc-shaped clamp 175 on the same side.
Referring to fig. 2, 13 and 14, the conversion mechanism 18 includes a second contact switch 181, a speed reducing motor 182, a rotating frame 183, a second electric push rod 184 and a third electric push rod 185, the second contact switch 181 is disposed on the front left side of the second support frame 2, the second contact switch 181 is disposed on the right side of the first contact switch 171, the speed reducing motor 182 is fixedly connected to the upper left side of the first support frame 1 through a bolt, the rotating frame 183 is rotatably connected to the upper left side of the first support frame 1, the lower left side of the rotating frame 183 is connected to an output shaft of the speed reducing motor 182, the second electric push rod 184 is disposed inside the rotating frame 183, a telescopic rod of the second electric push rod 184 is connected to the semiconductor laser source 4, the third electric push rod 185 is fixedly connected to the lower rear side of the first support frame 1 through a bolt, and the front side of the telescopic rod 185 is connected to the right side of the fourth lens 15.
The arc-shaped clamp 175 is manually moved to the outer side by people, the first spring 176 is stretched at the moment, then the sample is placed between the arc-shaped clamps 175, the arc-shaped clamps 175 are loosened, the first spring 176 is reset at the moment, the arc-shaped clamps 175 are driven to move and reset to the inner side, so that the sample is clamped by the arc-shaped clamps 176, then the power main switch is pressed to electrify the device, the first contact switch 171 is pressed, the control module controls the telescopic rod of the first electric push rod 173 to contract for two seconds, the telescopic rod of the first electric push rod 173 drives the sliding frame 174 to move to the left, further, the arc-shaped clamps 175 and the sample are driven to move to the left, so that the sample moves to the upper part of the fourth lens 15, then the semiconductor laser light source 4 is manually started, the light source emitted by the semiconductor laser source 4 passes through the beam expanding collimator 5, the beam collimator 5 expands the light source into two light sources, one beam is an object beam, and the other beam is a reference beam, then two light sources pass through a fourth lens 15 to irradiate on a sample, people can adjust a microscope objective group 7 to adjust to a required multiple, then the sample is magnified and imaged through the microscope objective group 7, the two light sources irradiate on the sample, pass through the microscope objective group 7 to irradiate on a first lens 8 and then irradiate on an optical wedge 9 through the first lens 8, a parallel light beam carrying object information is refracted and reflected by the optical wedge 9 and then divided into two parallel light beams with a certain included angle, the two light beams pass through a second lens 11 and can obtain frequency spectrum information of the two light beams on a Fourier frequency spectrum plane of the second lens 11, the light beams pass through a filter 12, the filter 12 reserves the bottom surface reflected light of the optical wedge 9 of low-frequency information as a reference light wave, also reserves the inclined surface reflected light of the optical wedge 9 of all the frequency spectrum information as an object wave, and performs Fourier inverse transformation on the filtered reference light wave and the object wave through a third lens 13, the reference light wave and the object light wave have an included angle and satisfy the coherence condition, so that interference can occur on the target surface of the imaging detector 14 to form an off-axis hologram, the off-axis hologram is recorded by the imaging detector 14, and the off-axis hologram captured by the imaging detector 14 is reconstructed by adopting a numerical reconstruction algorithm well known in the digital holography field, so that the phase information of the measured object can be obtained; people manually move the non-polarization beam splitter prism 16 to enable the non-polarization beam splitter prism 16 to be positioned above the first lens 8, the second contact switch 181 is pressed down, the control module controls the output shaft of the speed reducing motor 182 to rotate 90 degrees, meanwhile, the control module also controls the telescopic rod of the third electric push rod 185 to contract for one second, the telescopic rod of the third electric push rod 185 drives the fourth lens 15 to move backwards, the output shaft of the speed reducing motor 182 drives the rotating frame 183 to rotate 90 degrees, further, the second electric push rod 184, the semiconductor laser source 4 and the beam expanding collimator 5 are driven to rotate 90 degrees, the control module also controls the telescopic rod of the second electric push rod 184 to contract for two seconds after two seconds, the telescopic rod of the second electric push rod 184 drives the semiconductor laser source 4 and the beam expanding collimator 5 to move backwards, so that the light source emitted by the semiconductor laser source 4 irradiates on the non-polarization beam splitter prism 16, and the non-polarization beam splitter prism 16 refracts the light source, a light source enters the microscope objective group 7 through the first lens 8 to irradiate a sample, and then the sample is magnified and imaged by the microscope objective group 7; the light reflected by the sample enters the first lens 8 and irradiates on the non-polarization beam splitter 16, the reflected light penetrates through the non-polarization beam splitter 16 and irradiates on the optical wedge 9, people manually rotate the optical wedge 9 to change the incident angle and simulate the beam splitting property, meanwhile, the angle of the optical wedge 9 is adjusted, the refraction angle of the light can be adjusted, then the light is refracted on the second lens 11 through the optical wedge 9, the spectrum information of the two beams of light can be obtained on the Fourier spectrum surface of the second lens 11, the light beam penetrates through the filter 12, the filter 12 keeps the reflected light of the bottom surface of the optical wedge 9 of the low-frequency information as the reference light, the light reflected by the inclined surface of the optical wedge 9 of all the spectrum information is kept as the object light, the filtered reference light and the filtered object light are subjected to Fourier inverse transformation through the third lens 13, the reference light and the object light have an included angle and satisfy the coherence condition, so that the interference can occur on the target surface of the imaging detector 14, an off-axis hologram is formed and recorded by the imaging detector 14, the off-axis hologram captured by the imaging detector 14 is reconstructed by adopting a numerical reconstruction algorithm well known in the digital holography field, so that the phase information of the measured object can be obtained, a larger off-axis interference angle can be obtained while a smaller shearing amount is kept, then the first contact switch 171 is pressed again, the control module controls the telescopic rod of the first electric push rod 173 to extend for two seconds to reset, the telescopic rod of the first electric push rod 173 drives the sliding frame 174 to move rightwards, the arc-shaped clamp 175 and the sample are further driven to move rightwards, then people take the sample away, then the second contact switch 181 is pressed, the control module controls the output shaft of the speed reducing motor 182 to rotate for 90 degrees to reset, the output shaft of the speed reducing motor 182 drives the rotating frame 183, the second electric push rod 184, the semiconductor laser light source 4 and the beam expanding collimator 5 to rotate for 90 degrees to reset, make semiconductor laser light source 4 joint with elastic fixture 101 again, the first lens 8 of this device, second lens 11 and third lens 13 are adjustable, make things convenient for people to adjust the angle of photorefraction, dust cover 3 can block the dust, avoid the dust to influence the experimental result, when need not use, press the power master switch with this device outage can, the result can all be surveyed to above-mentioned two kinds of methods, thereby can improve the degree of accuracy of experiment, guarantee the rationality, two kinds of methods can make the experimental result stable, and the gained result can refer to each other, thereby it is favourable to follow-up adjustment.
Example 2
On the basis of embodiment 1, please refer to fig. 1, 15 and 16, further include stable reference mechanism 19, stable reference mechanism 19 includes locating rack 191, second spring 192, wedge block 193, clamping rack 194 and clamping plate 195, locating rack 191 is connected to the top of two arc-shaped clamps 175 in a sliding manner, two second springs 192 are connected to the two locating rack 191 and arc-shaped clamps 175 on the same side, wedge block 193 is welded to the outer side of the upper portion of two locating rack 191, clamping rack 194 is welded to the bottom of two arc-shaped clamps 175, two clamping plates 195 are welded to the lower front side of converter 6, wedge block 193 moves leftwards and can contact clamping plate 195.
People put the sample between screens frame 194, arc anchor clamps 175 moves left and drives the sample, spacer 191, wedge 193 and screens frame 194 move left, move left to when clamping plate 195 contacts when wedge 193, make wedge 193 move down, and then drive spacer 191 move down, second spring 192 is compressed this moment, make spacer 191 fix a position the sample, so, can avoid the sample to shake the influence gained result and lead to the error to appear, when arc anchor clamps 175 drives the sample, spacer 191, wedge 193 and screens frame 194 move right, second spring 192 resets this moment, it resets to drive spacer 191 and wedge 193 up removal, make spacer 191 loosen the sample.
Referring to fig. 3 and 17, the polarization beam splitter further includes a pushing mechanism 20, the pushing mechanism 20 includes a fixing frame 201, a first photoelectric sensor 202 and a fourth electric push rod 203, the fixing frame 201 is welded to the left rear side of the upper portion of the connecting frame 103, the first photoelectric sensor 202 is disposed on the left upper side of the fixing frame 201, the fourth electric push rod 203 is fixedly connected to the right rear side of the connecting frame 103 through a bolt, and the front side of the telescopic rod of the fourth electric push rod 203 is connected to the right side of the non-polarization beam splitter prism 16.
When the rotating frame 183 rotates by 90 degrees, the first photoelectric sensor 202 detects that the light is dark and reaches a preset value, the control module controls the telescopic rod of the fourth electric push rod 203 to extend for two seconds and then close, the telescopic rod of the fourth electric push rod 203 drives the non-polarization beam splitter prism 16 to move forwards, so that people do not need to manually control the non-polarization beam splitter prism 16 to move, when the rotating frame 183 passes through the first photoelectric sensor 202, the first photoelectric sensor 202 detects that the light is bright and returns to the initial value without sending a signal, when the rotating frame 183 is reversed and reset at 90 degrees, the first photoelectric sensor 202 detects that the light is dark and reaches the preset value again, the control module controls the telescopic rod of the fourth electric push rod 203 to contract for two seconds and close after resetting, thereby driving the non-polarization beam splitter prism 16 to move backward and reset, when the rotating frame 183 passes through the first photoelectric sensor 202, the first photosensor 202 detects that the light is brighter and returns to the initial value, and does not send a signal.
Referring to fig. 3, 18 and 19, the damping device further includes an anti-deviation mechanism 21, the anti-deviation mechanism 21 includes a fifth electric push rod 211, rubber clamping blocks 212 and a distance sensor 213, the fifth electric push rod 211 is fixedly connected to the left and right sides of the middle portion of the connecting frame 103 through bolts, the rubber clamping blocks 212 are respectively arranged at the bottoms of the telescopic rods of the fifth electric push rods 211, the two rubber clamping blocks 212 are respectively contacted with the damping rotating shaft 10 after moving, and the distance sensor 213 is arranged inside the arc-shaped clamp 175 at the front side.
People have adjusted the angle of light wedge 9 in advance, locating rack 191 of front side moves down to when being close to with distance sensor 213, distance sensor 213 detects and reaches the default with the distance between the locating rack 191 of front side, control module control fifth electric putter 211 telescopic link extension one second, fifth electric putter 211 telescopic link drives rubber clamp 212 and moves down, make rubber clamp 212 carry on spacingly to damping pivot 10, avoid light wedge 9 because this device shakes when the function, and then influence the refraction angle of light source, when locating rack 191 of front side up moves and resets, distance sensor 213 detects and gets back to the initial value with the distance between the locating rack 191 of front side, control module control fifth electric putter telescopic link 211 contracts one second after one second and resets, fifth electric putter 211 drives rubber clamp 212 and moves up and resets.
Referring to fig. 3 and 20, the three-point locking mechanism 22 is further included, the three-point locking mechanism 22 includes a pressure sensor 221, a fixing plate 222, a sixth electric push rod 223 and a push block 224, the pressure sensor 221 is disposed on the right side of the right rubber clamping block 212, the fixing plates 222 are symmetrically disposed on the upper sides of the two fifth electric push rods 211 in a front-back manner, the sixth electric push rods 223 are disposed on the lower sides of the four fixing plates 222, and the push blocks 224 are disposed on the inner sides of the telescopic rods of the four sixth electric push rods 223.
When the right rubber clamping block 212 moves downwards, the pressure sensor 221 is driven to move downwards, the right rubber clamping block 212 moves downwards to extrude the damping rotating shaft 10, the pressure sensor 221 detects that the pressure reaches a preset value, the control module controls the telescopic rod of the sixth electric push rod 223 to extend for one second and then close, the telescopic rod of the sixth electric push rod 223 drives the push block 224 to move inwards, so that the pushing block 224 presses the rubber clamping block 212, so that the rubber clamping block 212 locks the damping rotating shaft 10, and thus, the fixing effect is better, when the distance sensor 213 detects that the distance between the positioning frame 191 at the front side returns to the initial value, the control module also controls the sixth electric push rod 223 telescopic rod to contract for one second and then close after resetting, the sixth electric push rod 223 telescopic rod drives the push block 224 to move outwards and reset, so that the pushing block 224 releases the rubber clamping block 212, and then the right rubber clamping block 212 drives the pressure sensor 221 to move upwards for resetting.
Referring to fig. 3, 21 and 22, the dust removing device 23 further includes a second photoelectric sensor 231, a fan 232, an air deflector 233 and a torsion spring 234, the second photoelectric sensor 231 is disposed at the rear side of the inside of the right rubber clamping block 212, the fan 232 is fixedly connected to the middle of the connecting frame 103 through a bolt, the air deflector 233 is hinged to the front lower side of the fan 232, and the torsion spring 234 is connected between the left side and the right side of the front side of the air deflector 233 and the fan 232.
When the rubber clamping block 212 on the right side moves downwards, the second photoelectric sensor 231 is driven to move downwards, the second photoelectric sensor 231 is in contact with the damping rotating shaft 10, the second photoelectric sensor 231 detects that light rays are darker and reach a preset value, the control module controls the fan 232 to start, the wind power of the fan 232 drives the air guide plate 233 to rotate, at the moment, the torsion spring 234 deforms, the air guide plate 233 guides wind, and the wind removes dust on the optical wedge 9, so that the dust can be prevented from influencing the normal work of the optical wedge 9, after the rubber clamping block 212 on the right side drives the second photoelectric sensor 231 to move upwards and reset, the photoelectric sensor detects that the light rays are brighter and returns to an initial value, the control module controls the fan 232 to stop, at the moment, the torsion spring 234 resets, and drives the air guide plate 233 to reversely reset.
Referring to fig. 10, 23 and 24, the optical wedge off-axis digital holographic microscope further includes a control box 172, the control box 172 is disposed on the left front side of the second support frame 2, the control box 172 is located on the lower side of the first contact switch 171, the control box 172 includes a storage battery, a power module and a control module, the storage battery supplies power to the whole optical wedge 9-based common-path off-axis digital holographic microscope, an output end of the storage battery is electrically connected with the power module, the power module is connected with a power main switch through a circuit, and the power module is electrically connected with the control module; the control module is connected with a DS1302 clock circuit and a 24C02 circuit; pressure sensor 221, first photoelectric sensor 202, second photoelectric sensor 231, distance sensor 213, first contact switch 171 and second contact switch 181 all pass through electric connection with control module, gear motor 182 passes through direct current motor with control module and just reverses the module to be connected, and fan 232, first electric putter 173, second electric putter 184, third electric putter 185, fourth electric putter 203, fifth electric putter 211 and sixth electric putter 223 all pass through relay control module with control module and are connected.
The above description is only an embodiment of the present invention, and not intended to limit the scope of the present invention, and all modifications of equivalent structures and equivalent processes, which are made by the present specification, or directly or indirectly applied to other related technical fields, are included in the scope of the present invention.
Claims (9)
1. A common-path off-axis digital holographic microscopic device based on an optical wedge comprises a first support frame (1), elastic clamps (101), a connecting frame (103), a second support frame (2), semiconductor laser light sources (4), a converter (6), a microscopic objective group (7), a first lens (8), the optical wedge (9) and a damping rotating shaft (10), wherein the rear side of the first support frame (1) is connected with the second support frame (2), the lower side of the first support frame (1) is connected with three elastic clamps (101), the semiconductor laser light sources (4) are clamped between the elastic clamps (101),
the top of the first support frame (1) is connected with a connecting frame (103), a converter (6) is connected between the front lower side of the connecting frame (103) and the rear upper side of the first support frame (1), the top of the converter (6) is rotatably connected with a microscope objective group (7), the microscope objective group (7) consists of three objective lenses with different multiples, the middle of the connecting frame (103) is rotatably connected with a first lens (8), the upper part of the connecting frame (103) is rotatably connected with two damping rotating shafts (10), and an optical wedge (9) is connected between the inner sides of the two damping rotating shafts (10), the device is characterized by further comprising a dust cover (3), a beam expanding collimator (5), a second lens (11), a filter (12), a third lens (13), an imaging detector (14), a fixing rod (102), a guide frame (104), a fourth lens (15), a non-polarization beam splitting prism (16), a clamping mechanism (17) and a conversion mechanism (18), the upper part of the second support frame (2) is provided with a dust cover (3) for blocking dust, the upper part of the semiconductor laser light source (4) is provided with a beam expanding collimator (5), one side of the upper part of the connecting frame (103) close to the optical wedge (9) is rotatably connected with a second lens (11), one side of the upper part of the connecting frame (103) close to the second lens (11) is rotatably connected with a filter (12), one side of the upper part of the connecting frame (103) close to the filter (12) is rotatably connected with a third lens (13), the front side of the upper part of the connecting frame (103) is connected with an imaging detector (14), the lower side of the first support frame (1) is connected with two fixing rods (102), a fourth lens (15) is slidably connected between the front sides of the two fixing rods (102), the lower side of the connecting frame (103) is connected with two guide frames (104), and a non-polarization splitting prism (16) is slidably connected between the rear sides of the two guide frames (104), a first support frame (1) and
a clamping mechanism (17) for clamping the sample is arranged between the second support frames (2), a conversion mechanism (18) for driving the semiconductor laser light source (4) to rotate is arranged between the first support frame (1) and the second support frame (2), and the part of the conversion mechanism (18) is connected with the fourth lens (15).
2. The wedge-based, common-path, off-axis digital holographic microscopy apparatus of claim 1, the clamping mechanism is characterized in that the clamping mechanism (17) comprises a first contact switch (171), a first electric push rod (173), a sliding frame (174), arc-shaped clamps (175) and first springs (176), the first contact switch (171) is arranged on the front left side of the second support frame (2), the first electric push rod (173) is arranged on the lower rear side of the first support frame (1), the sliding frame (174) is connected to the lower right side of the first support frame (1) in a sliding mode, the rear right side of the sliding frame (174) is connected with the right side of the first electric push rod (173), the two arc-shaped clamps (175) used for clamping a sample are connected to the sliding frame (174) in a sliding mode, the first springs (176) are symmetrically arranged on the front and back of the left side and the right side of the middle of the sliding frame (174), and the outer sides of the four first springs (176) are connected with the arc-shaped clamps (175) on the same side.
3. The optical wedge-based common-path off-axis digital holographic microscopy device as claimed in claim 2, wherein the switching mechanism (18) comprises a second contact switch (181), a speed reducing motor (182), a rotating frame (183), a second electric push rod (184) and a third electric push rod (185), the second contact switch (181) is arranged on the front left side of the second support frame (2), the speed reducing motor (182) is connected to the upper left side of the first support frame (1), the rotating frame (183) is rotatably connected to the upper left side of the first support frame (1), the lower left side of the rotating frame (183) is connected to an output shaft of the speed reducing motor (182), the second electric push rod (184) is arranged inside the rotating frame (183), a telescopic rod of the second electric push rod (184) is connected to the semiconductor laser light source (4), the lower rear side of the first support frame (1) is connected to the third electric push rod (185), the front side of the telescopic rod of the third electric push rod (185) is connected with the right side of the fourth lens (15).
4. A wedge-based, common-path, off-axis digital holographic microscopy apparatus as defined in claim 3, the device is characterized by further comprising a stable reference mechanism (19) for fixing the sample, wherein the stable reference mechanism (19) comprises a positioning frame (191), a second spring (192), a wedge block (193), a clamping frame (194) and a clamping plate (195), the tops of two arc-shaped clamps (175) are all connected with the positioning frame (191) for fixing the sample in a sliding mode, two second springs (192) are connected between the two positioning frames (191) and the arc-shaped clamps (175) on the same side, the outer sides of the upper portions of the two positioning frames (191) are all connected with the wedge block (193), the bottoms of the two arc-shaped clamps (175) are all connected with the clamping frame (194), the lower side of the converter (6) is connected with the two clamping plates (195), and the wedge block (193) can be contacted with the clamping plate (195) when moving leftwards.
5. The common-path off-axis digital holographic microscopy device based on the optical wedge as claimed in claim 4, further comprising a pushing mechanism (20) for driving the non-polarization beam splitter prism (16) to move, wherein the pushing mechanism (20) comprises a fixing frame (201), a first photoelectric sensor (202) and a fourth electric push rod (203), the fixing frame (201) is connected to the left rear side of the upper portion of the connecting frame (103), the first photoelectric sensor (202) is arranged on the left upper side of the fixing frame (201), the fourth electric push rod (203) is connected to the right rear side of the upper portion of the connecting frame (103), and the front side of a telescopic rod of the fourth electric push rod (203) is connected to the right side of the non-polarization beam splitter prism (16).
6. The common-path off-axis digital holographic microscopy device based on the optical wedge as claimed in claim 5, further comprising an anti-deviation mechanism (21) for fixing the damping rotating shaft (10), wherein the anti-deviation mechanism (21) comprises a fifth electric push rod (211), rubber clamping blocks (212) and distance sensors (213), the fifth electric push rods (211) are connected to the left and right sides of the middle of the connecting frame (103), the rubber clamping blocks (212) for fixing the damping rotating shaft (10) are arranged at the bottoms of the telescopic rods of the two fifth electric push rods (211), and the distance sensors (213) are arranged inside the arc-shaped clamp (175) on the front side.
7. The common-path off-axis digital holographic microscopy device based on the optical wedge as claimed in claim 6, further comprising a three-point locking mechanism (22) for assisting the rubber clamping block (212) to fix the damping rotating shaft (10), wherein the three-point locking mechanism (22) comprises a pressure sensor (221), a fixing plate (222), a sixth electric push rod (223) and a pushing block (224), the pressure sensor (221) is arranged on the right side of the rubber clamping block (212) on the right side, the fixing plates (222) are symmetrically arranged on the upper sides of the two fifth electric push rods (211) in a front-back mode, the sixth electric push rods (223) are arranged on the lower sides of the four fixing plates (222), and the pushing blocks (224) for pushing the rubber clamping block (212) are arranged on the inner sides of telescopic rods of the four sixth electric push rods (223).
8. The common-path off-axis digital holographic microscopy device based on the optical wedge as claimed in claim 7, further comprising a dust removing mechanism (23) for removing dust from the optical wedge (9), wherein the dust removing mechanism (23) comprises a second photoelectric sensor (231), a fan (232), an air deflector (233) and a torsion spring (234), the second photoelectric sensor (231) is arranged on the rear side inside the rubber clamping block (212) on the right side, the fan (232) is connected to the middle of the connecting frame (103), the rotary air deflector (233) is connected to the front lower side of the fan (232), and the torsion spring (234) is connected between the left side and the right side of the front side of the air deflector (233) and the fan (232).
9. The optical wedge-based common-path off-axis digital holographic microscopy device is characterized by further comprising a control box (172), wherein the control box (172) is arranged on the left front side of the second support frame (2), the control box (172) comprises a storage battery, a power supply module and a control module, the storage battery supplies power to the whole optical wedge (9) -based common-path off-axis digital holographic microscopy device, the output end of the storage battery is electrically connected with the power supply module, a power main switch is connected to the power supply module through a circuit, and the power supply module is electrically connected with the control module; the control module is connected with a DS1302 clock circuit and a 24C02 circuit; pressure sensor (221), first photoelectric sensor (202), second photoelectric sensor (231), distance sensor (213), first contact switch (171) and second contact switch (181) all pass through electric connection with control module, gear motor (182) pass through direct current motor with control module and just reverse the module and be connected, fan (232), first electric putter (173), second electric putter (184), third electric putter (185), fourth electric putter (203), fifth electric putter (211) and sixth electric putter (223) all pass through relay control module with control module and are connected.
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Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN102576209A (en) * | 2009-10-08 | 2012-07-11 | 布鲁塞尔大学 | Off-axis interferometer |
| CN108008616A (en) * | 2017-11-14 | 2018-05-08 | 清华大学 | Compact is without lens accurate light path off-axis digital holography imaging system and method altogether |
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Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102576209A (en) * | 2009-10-08 | 2012-07-11 | 布鲁塞尔大学 | Off-axis interferometer |
| CN108008616A (en) * | 2017-11-14 | 2018-05-08 | 清华大学 | Compact is without lens accurate light path off-axis digital holography imaging system and method altogether |
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