CN114113621A - Urine guanine nucleotide binding protein subunit alpha-13 and application of polypeptide fragment thereof in burn - Google Patents
Urine guanine nucleotide binding protein subunit alpha-13 and application of polypeptide fragment thereof in burn Download PDFInfo
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- CN114113621A CN114113621A CN202010863141.1A CN202010863141A CN114113621A CN 114113621 A CN114113621 A CN 114113621A CN 202010863141 A CN202010863141 A CN 202010863141A CN 114113621 A CN114113621 A CN 114113621A
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- binding protein
- burn
- urine
- guanine nucleotide
- nucleotide binding
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Abstract
The invention provides urine Guanine nucleotide binding protein subunit alpha-13 (Guanine nucleotide binding protein subBunit alpha-13) and application of a polypeptide fragment thereof, in particular to application of urine Guanine nucleotide binding protein subunit alpha-13 and a polypeptide fragment thereof in preparation of preparations for burn diagnosis, differential diagnosis, burn area and degree evaluation, treatment effect evaluation, monitoring, prognosis evaluation, mechanism research and the like. The burn is an important common wound in daily life, about 5000-100000 people burn in every 100 million people every year, according to the statistics of the world health organization, more than 30 million people die of burn patients all year around the world, and the survival rate of the serious burn treatment is still at a lower level. The present invention confirmed by studies that urine guanine nucleotide binding protein subunit alpha-13 and its polypeptide fragment are reduced in expression in burn patients compared to healthy persons (normal control group), and their content gradually decreases as the degree of burn worsens. Can be used for various purposes of detecting burn patients. The invention exerts the advantages of noninvasive acquisition, large-scale repeated sampling and convenient preservation of the urine specimen, and utilizes the urine specimen to detect the urine guanine nucleotide binding protein subunit alpha-13 and the polypeptide fragment thereof.
Description
Technical Field
The invention relates to a new application of urine guanine nucleotide binding protein subunit alpha-13 and a polypeptide fragment thereof, in particular to application of urine guanine nucleotide binding protein subunit alpha-13 and a polypeptide fragment thereof in burn diagnosis, differential diagnosis, burn area and degree evaluation, treatment effect evaluation, monitoring, prognosis evaluation, mechanism research and the like.
Background
Burn refers to the injury of skin, tissue and even deep viscera of human body caused by chemical substances such as flame, high-temperature gas, scorching solid or liquid, radioactive rays, electric energy, strong acid and strong alkali, etc., and is a systemic comprehensive disease. After burn, a large amount of reactions such as necrosis, infection, shock, blood coagulation dysfunction and the like of wound tissues can cause a series of pathophysiological changes of organisms. Burns of different degrees have different influences on human bodies, serious burns can damage the environment in the human bodies, the burn patients have pathophysiological changes of complexity of various systems, and relevant detection indexes can correspondingly change along with the difference of the severity of the burns. Timely detection of changes in these indices can provide valuable reference for clinicians in many areas, such as disease diagnosis, disease judgment, treatment selection, and patient prognosis assessment.
However, patients with severe burns have poor skin integrity, and clinical use of hematological tests as invasive tests on the skin in such patients has presented difficulties, and repeated blood draw tests can also exacerbate patient pain. Urine as ultrafiltrate of blood contains abundant biological information, and the collection process has the advantages of non-invasive and convenient operation, and the like, which is particularly obvious in the detection of burn patients. The biomarker which is helpful for burn diagnosis and reflects disease change is searched in urine, so that the life quality and compliance of burn patients can be improved, the pain of blood collection for many times is relieved, and a reference basis which is favorable for disease diagnosis and treatment is better provided for clinicians.
Guanine nucleotide-binding protein subunit alpha-13 (G alpha-13) is an important member of the G protein family. G proteins, also known as GTP-binding proteins, are a class of signal transduction proteins, including about more than 40 types, that can transmit external signals into cells and activate various downstream signaling pathways. G alpha-13 is encoded by GNA13 gene, has molecular functions of coupling and binding with D5 dopamine receptor, G protein and metal ion, and is involved in a plurality of biological processes such as platelet activation, phospholipase D activity activation and positive regulation of plasma calcium ion concentration. The content of G alpha-13 in urine of burn patients in the study is reduced in comparison with that of healthy people, and has a certain correlation with the burn degree, and the more serious the burn degree is, the lower the content of the protein in urine is.
Compared with the common clinical blood sample, the urine can be collected in a non-invasive, continuous and large amount; without homeostatic regulation, more various and larger changes can be accumulated, and many pathophysiological changes of the body can be reflected in urine. Some protein polypeptides with relatively small molecular weight, such as hormones and cytokines, are excreted into urine quickly after entering blood, and the probability that the proteins and polypeptides are detected in urine is much higher than that in blood; before urine is collected, a possible protein degradation process in urine is completed, so that urine protein can be kept stable for a longer time. In order to relieve the pain of multiple blood sampling of burn patients, the experiment is expected to realize the diagnosis and disease monitoring of the burn patients by painless, convenient, quick and easily repeated urine detection through the research of urine protein or polypeptide on the basis of the methodology exploration of the early stage, and also lays a foundation for the further research of the urine polypeptide detection kit.
Disclosure of Invention
The invention aims to provide application of urine guanine nucleotide binding protein subunit alpha-13 and polypeptide fragments thereof in preparation of preparations for burn diagnosis, differential diagnosis, burn area and degree evaluation, treatment effect evaluation, monitoring, prognosis evaluation, mechanism research and the like.
Preferably, the amino acid sequence of subunit alpha-13 of the urine guanine nucleotide binding protein is shown in SEQ ID NO.1 (MADFLPSRSV LSVCFPGCLL TSGEAEQQRK SKEIDKCLSR EKTYVKRLVK ILLLGAGESG KSTFLKQMRI IHGQDFDQRA REEFRPTIYS NVIKGMRVLV DAREKLHIPW GDNSNQQHGD KMMSFDTRAP MAAQGMVETR VFLQYLPAIR ALWADSGIQN AYDRRREFQL GESVKYFLDN LDKLGEPDYI PSQQDILLAR RPTKGIHEYD FEIKNVPFKM VDVGGQRSER KRWFECFDSV TSILFLVSSS EFDQVLMEDR LTNRLTESLN IFETIVNNRV FSNVSIILFL NKTDLLEEKV QIVSIKDYFL EFEGDPHCLR DVQKFLVECF RNKRRDQQQK PLYHHFTTAI NTENIRLVFR DVKDTILHDN LKQLMLQ); or an amino acid sequence which is derived from the amino acid sequence shown in SEQ ID NO.1 and has the same function with the amino acid sequence shown in SEQ ID NO. 1.
Preferably, the preparation is a kit for detecting guanine nucleotide binding protein subunit alpha-13 and polypeptide fragments thereof in urine of a burn patient.
Preferably, the kit includes an immunological method of antigen-antibody reaction and a kit thereof such as one or more of an aptamer antibody or an antibody fragment capable of specifically binding to guanine nucleotide binding protein subunit alpha-13 and polypeptide fragments thereof.
Preferably, the detection method comprises methods such as mass spectrometry for directly detecting guanine nucleotide binding protein subunit alpha-13 and polypeptide fragments thereof and related kits thereof.
Preferably, the detection method comprises a related nucleic acid detection method for directly detecting the guanine nucleotide binding protein subunit alpha-13 and the polypeptide fragment thereof, and a related kit thereof.
Preferably, the kit further comprises a component selected from the group consisting of: the kit comprises a solid phase carrier, a diluent, a reference substance, a standard substance, a quality control substance, a detection antibody, a second antibody diluent, a luminescent reagent, a washing solution, a color development solution and a stop solution, wherein the solid phase carrier is any one or a combination of a plurality of the solid phase carrier, the diluent, the reference substance, the standard substance, the quality control substance, the detection antibody, the second antibody and the second antibody diluent.
Preferably, the standard comprises a guanine nucleotide binding protein subunit alpha-13 standard and a humanized tag antibody standard; preferably, the quality control product comprises: a guanine nucleotide binding protein subunit alpha-13 quality control product and a humanized label antibody quality control product; preferably, the solid support comprises: particles, microspheres, glass slides, test strips, plastic beads, liquid phase chips, micro-porous plates or affinity membranes and other carriers with the same functions.
Preferably, the solid phase carrier is made of any one of polyvinyl chloride, polystyrene, polyacrylamide and cellulose, and has similar functions.
The inventor firstly collects urine samples of healthy people and patients with different burn degrees, centrifugates for 5min at 4000r/min, absorbs supernatant, determines the concentration of extracted protein by a Bradford method, and carries out SDS-PAGE enzymolysis. The Label-free mass spectrometry of the urine samples was performed by the OrbitrapFasion type mass spectrometer. And performing quantitative calculation on data obtained in the mass spectrum of the burn group and the normal control group. The differential polypeptide is screened by using the difference of protein expression amount more than 1.5 times and P <0.05 as a reference standard through statistical test. Then, the inventor identifies the differential polypeptide with statistical significance, and utilizes database retrieval to obtain the differential protein guanine nucleotide binding protein subunit alpha-13.
The research proves that compared with healthy people, the guanine nucleotide binding protein subunit alpha-13 and the polypeptide fragment thereof are low expressed in urine of burn patients, and are reduced along with the aggravation of the burn degree, and the invention has better consistency with clinical diagnosis. Therefore, urine guanine nucleotide binding protein subunit alpha-13 and polypeptide fragments thereof can be used for auxiliary diagnosis or disease condition monitoring of burn.
The invention exerts the advantages of noninvasive acquisition, large-scale repeated sampling and convenient preservation of the urine specimen, and utilizes the urine specimen to detect the urine guanine nucleotide binding protein subunit alpha-13 and the polypeptide fragment thereof.
In order to make the aforementioned and other objects, features and advantages of the present invention comprehensible, preferred embodiments accompanied with figures are described in detail below.
Drawings
FIG. 1 is a graph showing the content of urine guanine nucleotide binding protein subunit alpha-13 and its polypeptide fragments in burn group and healthy control group.
Detailed Description
Example 1Collection and processing of urine specimens
Burn patients were selected as the burn group, and contemporary physical examiners were selected as the normal control group. 30ml samples of fresh morning urine were collected from each group of subjects after admission, and those who failed to urinate normally collected their morning urine from their catheters and placed in dry, clean containers. Centrifuging the collected urine specimen at 4000r/min for 5min, sucking supernatant, subpackaging 2ml per tube, and storing in a refrigerator at-80 ℃.
Example 2Mass spectrometry and screening of urine polypeptides
Extracting protein from urine sample, and determining the concentration of extracted protein. Mass spectrometry of urine samples was performed by orbitrapfuision type mass spectrometry. And performing quantitative calculation on data obtained in the mass spectrum of the experimental group and the normal control group. The comparison among groups adopts t-test to carry out differential analysis, and differential expression proteins are screened by using the difference of protein expression quantity more than 1.5 times and taking the statistical test that P <0.05 as a reference standard.
Example 3Identification and analysis of differential Polypeptides
The used database is a Unit _ Homo database, the generated mass spectrum original file is processed by MaxQuant software, and the retrieval parameter setting is shown in Table 1.
Compared with healthy people, the guanine nucleotide binding protein subunit alpha-13 is low expressed in urine of burn patients, the content of the guanine nucleotide binding protein subunit alpha-13 in urine of healthy control groups and burn groups is shown in figure 1, and the expression of the guanine nucleotide binding protein subunit alpha-13 in urine of normal control groups and burn groups has a significant difference and is reduced along with the aggravation of the burn degree.
Although the present invention has been described with respect to the preferred embodiments, it will be understood by those skilled in the art that various changes in form and details may be made therein without departing from the spirit and scope of the invention as defined by the appended claims.
Sequence listing
<110> Zhang Man
<120> urine guanine nucleotide binding protein subunit alpha-13 and application of polypeptide fragment thereof in burn
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<140> 20PG alpha-13-CN
<141> 2020-08-03
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<170> SIPOSequenceListing 1.0
<210> 1
<211> 377
<212> PRT
<213> Human Urine
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Met Ala Asp Phe Leu Pro Ser Arg Ser Val Leu Ser Val Cys Phe Pro
1 5 10 15
Gly Cys Leu Leu Thr Ser Gly Glu Ala Glu Gln Gln Arg Lys Ser Lys
20 25 30
Glu Ile Asp Lys Cys Leu Ser Arg Glu Lys Thr Tyr Val Lys Arg Leu
35 40 45
Val Lys Ile Leu Leu Leu Gly Ala Gly Glu Ser Gly Lys Ser Thr Phe
50 55 60
Leu Lys Gln Met Arg Ile Ile His Gly Gln Asp Phe Asp Gln Arg Ala
65 70 75 80
Arg Glu Glu Phe Arg Pro Thr Ile Tyr Ser Asn Val Ile Lys Gly Met
85 90 95
Arg Val Leu Val Asp Ala Arg Glu Lys Leu His Ile Pro Trp Gly Asp
100 105 110
Asn Ser Asn Gln Gln His Gly Asp Lys Met Met Ser Phe Asp Thr Arg
115 120 125
Ala Pro Met Ala Ala Gln Gly Met Val Glu Thr Arg Val Phe Leu Gln
130 135 140
Tyr Leu Pro Ala Ile Arg Ala Leu Trp Ala Asp Ser Gly Ile Gln Asn
145 150 155 160
Ala Tyr Asp Arg Arg Arg Glu Phe Gln Leu Gly Glu Ser Val Lys Tyr
165 170 175
Phe Leu Asp Asn Leu Asp Lys Leu Gly Glu Pro Asp Tyr Ile Pro Ser
180 185 190
Gln Gln Asp Ile Leu Leu Ala Arg Arg Pro Thr Lys Gly Ile His Glu
195 200 205
Tyr Asp Phe Glu Ile Lys Asn Val Pro Phe Lys Met Val Asp Val Gly
210 215 220
Gly Gln Arg Ser Glu Arg Lys Arg Trp Phe Glu Cys Phe Asp Ser Val
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Thr Ser Ile Leu Phe Leu Val Ser Ser Ser Glu Phe Asp Gln Val Leu
245 250 255
Met Glu Asp Arg Leu Thr Asn Arg Leu Thr Glu Ser Leu Asn Ile Phe
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Glu Thr Ile Val Asn Asn Arg Val Phe Ser Asn Val Ser Ile Ile Leu
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Phe Leu Asn Lys Thr Asp Leu Leu Glu Glu Lys Val Gln Ile Val Ser
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Ile Lys Asp Tyr Phe Leu Glu Phe Glu Gly Asp Pro His Cys Leu Arg
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Asp Val Gln Lys Phe Leu Val Glu Cys Phe Arg Asn Lys Arg Arg Asp
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Gln Gln Gln Lys Pro Leu Tyr His His Phe Thr Thr Ala Ile Asn Thr
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Glu Asn Ile Arg Leu Val Phe Arg Asp Val Lys Asp Thr Ile Leu His
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Asp Asn Leu Lys Gln Leu Met Leu Gln
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Claims (9)
1. Urine guanine nucleotide binding protein subunit alpha-13 and the application of the polypeptide fragment thereof in preparing preparations for burn diagnosis, differential diagnosis, burn area and degree evaluation, treatment effect evaluation, monitoring, prognosis evaluation, mechanism research and the like.
2. The use of claim 1, wherein the urine guanine nucleotide binding protein subunit α -13 has an amino acid sequence as set forth in SEQ ID No. 1; or an amino acid sequence which is derived from the amino acid sequence shown in SEQ ID NO.1 and has the same function with the amino acid sequence shown in SEQ ID NO. 1.
3. The use of claim 1, wherein the agent is a kit for detecting guanine nucleotide binding protein subunit α -13 and its polypeptide fragments in urine of burn patients.
4. The use of claim 3, wherein the kit comprises an immunological method of antigen-antibody reaction and kits thereof such as one or more of an aptamer antibody or an antibody fragment capable of specifically binding to guanine nucleotide binding protein subunit α -13 and polypeptide fragments thereof.
5. The use of claim 3, wherein the detection method comprises mass spectrometry and related kits for directly detecting guanine nucleotide binding protein subunit α -13 and its polypeptide fragments.
6. The use of claim 3, wherein the detection method comprises methods for directly detecting guanine nucleotide binding protein subunit α -13 and its polypeptide fragment or its related nucleic acid, and related kits.
7. The use according to claim 3, wherein the kit further comprises a component selected from the group consisting of: the kit comprises a solid phase carrier, a diluent, a reference substance, a standard substance, a quality control substance, a detection antibody, a second antibody diluent, a luminescent reagent, a washing solution, a color development solution and a stop solution, wherein the solid phase carrier is any one or a combination of a plurality of the solid phase carrier, the diluent, the reference substance, the standard substance, the quality control substance, the detection antibody, the second antibody and the second antibody diluent.
8. The use of claim 7, wherein the standard comprises a guanine nucleotide binding protein subunit α -13 standard, a humanized tag antibody standard; preferably, the quality control product comprises: a guanine nucleotide binding protein subunit alpha-13 control product and a humanized label antibody quality control product; preferably, the solid support comprises: particles, microspheres, glass slides, test strips, plastic beads, liquid phase chips, micro-porous plates or affinity membranes and other carriers with the same functions.
9. The use of claim 8, wherein the solid phase carrier is made of any one of polyvinyl chloride, polystyrene, polyacrylamide, cellulose, and carriers with similar functions.
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Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1315331A (en) * | 2000-03-24 | 2001-10-03 | 上海博德基因开发有限公司 | Polypeptide-subunit 55 of guanine nucleotide bindin and polynucleotide for coding it |
| CN111499615A (en) * | 2017-08-04 | 2020-08-07 | 斯基霍克疗法公司 | Methods and compositions for modulating splicing |
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1315331A (en) * | 2000-03-24 | 2001-10-03 | 上海博德基因开发有限公司 | Polypeptide-subunit 55 of guanine nucleotide bindin and polynucleotide for coding it |
| CN111499615A (en) * | 2017-08-04 | 2020-08-07 | 斯基霍克疗法公司 | Methods and compositions for modulating splicing |
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