CN114107009B - Cell pipetting gun - Google Patents

Abstract

The application provides a cell pipette, and relates to the technical field of biology. The scheme of the application is as follows: a cell pipette comprises a pipette body, a pipette body and a compression structure; the top end of the pipette cylinder body is provided with a compression structure for compressing air in the pipette cylinder body; the bottom of the pipette gun cylinder body is connected with a straw body communicated with the inside of the pipette gun cylinder body; an exhaust assembly for exhausting air in the pipette barrel is arranged at the top of the pipette barrel; the pipette body is provided with a blocking component which is used for isolating the pipette barrel from outside air. The application can pump cell mixed liquid with full range.

Description

A kind of cell pipette gun

technical field

The invention relates to the field of biotechnology, in particular to a cell pipette gun.

Background technique

Cell culture is also called cell cloning technology, and the formal term in biology is cell culture technology. No matter for the entire bioengineering technology or one of the biological cloning technologies, cell culture is an essential process, and cell culture itself is a large-scale cloning of cells. Cell culture technology can transform a single cell into simple single cell or rarely differentiated multi-cell through mass culture, which is an essential part of cloning technology, and cell culture itself is cell cloning.

At present, due to the presence of gas in the cell mixture when the pipette is pumping the cell mixture, the pipette barrel draws the cell mixture into the culture medium by using the pressure difference to make the cell mixture enter the inside of the pipette barrel and extract During the process, the pressure in the culture solution becomes smaller, and part of the gas in the culture solution is precipitated due to the pressure drop, which makes it impossible to draw a full range of cell mixture in the pipette.

Contents of the invention

The object of the present invention is to provide a cell pipette gun capable of pumping a full volume of cell mixture.

Embodiments of the present invention are achieved like this:

The embodiment of the present application provides a cell pipette gun, including a pipette gun barrel, a suction pipe body and a compression structure; the top of the pipette gun barrel is installed with a compression structure for compressing the air inside the pipette gun barrel; the pipette gun The bottom of the barrel is connected to the pipette body that is connected to the inside of the pipette gun barrel; the top of the pipette gun barrel is equipped with an exhaust assembly for exhausting the air inside the pipette gun barrel; A sealing component that isolates the body from the outside air.

In some embodiments of the present invention, the compression structure includes a push-pull rod and a compression substrate, the push-pull rod runs through the top of the pipette gun barrel, one end of the push-pull rod is connected to the compression substrate, the compression substrate is located in the pipette gun barrel, and the compression substrate The ring side of the pipette fits the inside wall of the pipette barrel.

In some embodiments of the present invention, an elastic rubber ring is sheathed on the annular side of the compression substrate.

In some embodiments of the present invention, a return spring is installed between the compression substrate and the pipette barrel, one end of the return spring is connected to the compression substrate, and the other end of the return spring is connected to the inner upper wall of the pipette barrel.

In some embodiments of the present invention, the exhaust assembly includes a rotating base plate and a first rotating member for rotating the rotating base plate, the rotating base plate is rotatably connected to the compression base plate, the compression base plate is provided with a first vent, and the rotation base plate is provided with a The first air port matches the second air port, and the top of the pipette barrel is provided with an arc-shaped sliding port for matching with the first rotating member.

In some embodiments of the present invention, an elastic rubber ring is sheathed on the ring side of the rotating substrate.

In some embodiments of the present invention, the above-mentioned blocking assembly includes a blocking shell and a roller. The straw body runs through the blocking shell. The blocking shell is provided with a rolling groove that cooperates with the roller. The connection groove, the level of one end of the connection groove is higher than the level of the other end of the connection groove.

In some embodiments of the present invention, an observation window is installed on the side wall of the pipette barrel.

In some embodiments of the present invention, the observation window is provided with scale lines from top to bottom.

Compared with the prior art, the embodiments of the present invention have at least the following advantages or beneficial effects:

A cell pipette gun, comprising a pipette gun barrel, a suction pipe body and a compression structure; the top of the pipette gun barrel is installed with a compression structure for compressing the air inside the pipette gun barrel; the bottom of the pipette gun barrel is connected The suction pipe body communicates with the inside of the pipette gun barrel; the top of the pipette gun barrel is equipped with an exhaust assembly for exhausting the air inside the pipette gun barrel; The blocking component.

In the above-mentioned embodiment, a cell pipette gun is composed of a pipette gun barrel, a straw body and a compression structure, wherein the upper part of the pipette gun barrel is installed with the above-mentioned compression structure, and the lower part of the pipette gun barrel is installed with the above-mentioned straw body, The compression structure can be used to compress the air in the pipette gun barrel. Specifically, when the staff extracts the cell mixture in the culture medium, the air in the pipette gun barrel is first discharged through the compression structure, and then the pipette gun The pipette body at the bottom of the cylinder is placed in the culture medium, and then the negative pressure is generated in the pipette gun cylinder through the compression structure, and the pipette gun cylinder absorbs the cell mixture into the culture medium through the pipette body. On the one hand, due to the mixing of cells There is gas in the liquid. When the pipette gun barrel draws the cell mixture into the culture medium, the pressure difference is used to make the cell mixture enter the inside of the pipette gun barrel. During the extraction process, the pressure in the culture medium becomes smaller, and some of the The gas is precipitated due to the problem of pressure drop. Second, when the pipette barrel is drawn too violently into the culture solution, the culture solution has not entered the pipette barrel in time and a short vacuum is generated, which will disappear afterwards. Third On the one hand, there is residual gas in the pipette barrel before pumping the cell mixture into the culture medium. The above problems will cause the staff to pump the cell mixture into the pipette barrel, resulting in excess gas in the pipette barrel , but the above-mentioned excess gas cannot be discharged and then the same volume of cell mixture can be extracted into the culture medium, resulting in the problem of low work efficiency. , if there is excess air in the pipette gun barrel, the above-mentioned excess air is exhausted to the outside of the pipette gun barrel through the exhaust assembly. During this process, the staff opens the blocking assembly to prevent the cell mixture in the pipette gun barrel From the pipette body to the outside, when all the air in the pipette gun barrel is exhausted to the outside, the staff will open the compression structure again, so that the pipette gun barrel will pump out the same volume of cell mixture as the excess air into the culture medium, making the pipette The liquid gun barrel is filled with the cell mixture, which avoids the problem of low working efficiency of the pipette barrel due to excess air inside the pipette gun barrel after the cell mixture is extracted.

In this embodiment, when the staff uses the cell pipette gun to extract the cell mixture into the culture medium, the pressure difference of the air is used to extract the cell mixture into the barrel of the pipette. On the one hand, due to the presence of gas in the cell mixture, When the pipette gun barrel draws the cell mixture into the culture medium, the pressure difference is used to make the cell mixture enter the inside of the pipette gun barrel. During the extraction process, the pressure in the culture medium becomes smaller, and part of the gas in the culture medium is reduced due to the pressure drop. In the second aspect, the pipette gun barrel is too violently drawn into the culture solution, causing the culture solution to not enter the pipette gun barrel in time, resulting in a short vacuum, which will then disappear. In the third aspect, the pipette gun Before the barrel pumps the cell mixture into the culture medium, there is a problem of residual gas, which will cause excess gas in the pipette gun barrel after pumping the cell mixture, and the cell mixture cannot be fully pumped. The staff can Exhaust the excess gas in the pipette gun barrel through the exhaust assembly, and then pass the compression structure to make the pipette gun barrel pump out the cell mixture of the same volume as the excess air into the culture medium, so that more cells can be extracted at one time The mixed solution improves work efficiency.

Description of drawings

In order to illustrate the technical solutions of the embodiments of the present invention more clearly, the accompanying drawings used in the embodiments will be briefly introduced below. It should be understood that the following drawings only show some embodiments of the present invention, and thus It should be regarded as a limitation on the scope, and those skilled in the art can also obtain other related drawings based on these drawings without creative work.

Fig. 1 is a schematic structural diagram of a cell pipette gun according to an embodiment of the present invention;

Fig. 2 is a schematic diagram of the inside of the barrel of the pipette gun according to the embodiment of the present invention;

Fig. 3 is a schematic diagram of the structure of the upper end of the pipette gun barrel according to the embodiment of the present invention;

Fig. 4 is a partial sectional view of a cell pipetting gun according to an embodiment of the present invention.

Marked in the figure: 1- pipette gun barrel, 2- push-pull rod, 3- suction pipe body, 4- block assembly, 5- first rotating part, 6- rotating base plate, 7- compression base plate, 8- return spring, 9 - the second air vent, 10 - the first air vent, 11 - the arc-shaped sliding port, 12 - the first connecting section, 13 - the second connecting section.

Detailed ways

In order to make the purpose, technical solutions and advantages of the embodiments of the present invention clearer, the technical solutions in the embodiments of the present invention will be clearly and completely described below in conjunction with the drawings in the embodiments of the present invention. Obviously, the described embodiments It is a part of embodiments of the present invention, but not all embodiments. The components of the embodiments of the invention generally described and illustrated in the figures herein may be arranged and designed in a variety of different configurations.

Accordingly, the following detailed description of the embodiments of the invention provided in the accompanying drawings is not intended to limit the scope of the claimed invention, but merely represents selected embodiments of the invention. Based on the embodiments of the present invention, all other embodiments obtained by persons of ordinary skill in the art without creative efforts fall within the protection scope of the present invention.

It should be noted that like numerals and letters denote similar items in the following figures, therefore, once an item is defined in one figure, it does not require further definition and explanation in subsequent figures.

In the description of the embodiments of the present invention, it should be noted that if the terms "center", "upper", "lower", "left", "right", "vertical", "horizontal", "inner", The orientation or positional relationship indicated by "outside" is based on the orientation or positional relationship shown in the drawings, or the orientation or positional relationship that is usually placed when the product of the invention is used, and is only for the convenience of describing the present invention and simplifying the description. It is not to indicate or imply that the device or element referred to must have a particular orientation, be constructed in a particular orientation, or operate in a particular orientation, and thus should not be construed as limiting the invention. In addition, the terms "first", "second", etc. are only used for distinguishing descriptions, and should not be construed as indicating or implying relative importance.

In addition, the appearance of the terms "horizontal", "vertical", "overhanging" etc. does not mean that the parts are absolutely horizontal or overhanging, but may be slightly inclined. For example, "horizontal" only means that its direction is more horizontal than "vertical", and it does not mean that the structure must be completely horizontal, but can be slightly inclined.

In the description of the embodiments of the present invention, it should also be noted that, unless otherwise specified and limited, the terms "setting", "installation", "connection" and "connection" should be interpreted in a broad sense, for example, It can be a fixed connection, a detachable connection, or an integral connection; it can be a mechanical connection or an electrical connection; it can be a direct connection or an indirect connection through an intermediary, and it can be the internal communication of two components. Those of ordinary skill in the art can understand the specific meanings of the above terms in the present invention according to specific situations.

Example

Please refer to Figure 1-Figure 3. This embodiment provides a cell pipette, including a pipette body 1, a straw body 3 and a compression structure; the top of the pipette body 1 is installed with a compression structure for compressing the air inside the pipette body 1; The bottom of the pipette gun barrel 1 is connected to the pipette body 3 that communicates with the inside of the pipette gun barrel 1; the top of the pipette gun barrel 1 is equipped with an exhaust assembly for discharging the internal air of the pipette gun barrel 1; the suction pipe body 3. A block assembly 4 for isolating the inside of the pipette gun barrel 1 from the outside air is installed.

In the above embodiment, a cell pipette consists of a pipette body 1, a pipette body 3 and a compression structure, wherein the upper part of the pipette body 1 is equipped with the compression structure, and the lower part of the pipette body 1 is installed The suction pipe body 3 and the compression structure can be used to compress the air in the pipette gun barrel 1. Specifically, when the staff extracts the cell mixture in the culture medium, the compression structure first compresses the air in the pipette gun barrel 1. The air is discharged, and then the pipette body 3 at the bottom of the pipette gun barrel 1 is placed in the culture solution, and then a negative pressure is generated in the pipette gun barrel 1 through the compression structure, and the pipette gun barrel 1 passes through the pipette body 3 Aspirating the cell mixture into the culture medium, on the one hand, due to the presence of gas in the cell mixture, the pipette barrel 1 draws the cell mixture into the culture medium by using the pressure difference to make the cell mixture enter the pipette barrel 1 Internally, the pressure in the culture solution becomes smaller during the extraction process, and part of the gas in the culture solution is precipitated due to the pressure drop. On the other hand, the pipette gun barrel 1 is too violently drawn into the culture solution, resulting in the culture solution not yet Into the pipette gun barrel 1 in time to generate a short-term vacuum, and then it will disappear. In the third aspect, there is residual gas before the pipette gun barrel 1 extracts the cell mixture into the culture medium. The above problems will cause the staff to remove the cells. After the mixed solution is pumped into the pipette gun barrel 1, there is excess gas in the pipette gun barrel 1, and the above-mentioned excess gas cannot be discharged and then the same volume of cell mixed solution is drawn into the culture medium, resulting in a loss of work efficiency. Low problem, in this application, after the pipette gun barrel 1 pumps the cell mixture in the culture medium into the interior, if there is excess air in the pipette gun barrel 1, the above-mentioned excess air will be discharged to the The outside of the pipette gun barrel 1, during this process, the staff opens the block assembly 4 to prevent the cell mixture in the pipette gun barrel 1 from flowing into the outside from the pipette body 3, when the air in the pipette gun barrel 1 is completely When it is discharged to the outside, the staff opens the compression structure again, so that the pipette gun barrel 1 pumps the same volume of cell mixture as the excess air into the culture medium, so that the pipette gun barrel 1 is filled with the cell mixture, avoiding the transfer After the liquid gun barrel 1 extracts the cell mixture, there is excess air inside, which causes the problem that the working efficiency of the pipette gun barrel 1 is low.

In this embodiment, when the staff draws the cell mixture into the culture medium through the cell pipette gun, the pressure difference of the air is used to draw the cell mixture into the pipette barrel 1. On the one hand, due to the presence of Gas, when the pipette gun barrel 1 draws the cell mixture into the culture medium, the pressure difference is used to make the cell mixture enter the inside of the pipette gun barrel 1, and the pressure in the culture medium becomes smaller during the extraction process, and part of the gas in the culture medium Precipitation due to the problem of pressure drop. Second, the pipette gun barrel 1 is too violently drawn into the culture solution, causing the culture solution to not enter the pipette gun barrel 1 in time to generate a short vacuum, which will then disappear. In the third aspect, there is a problem of residual gas before the pipette barrel 1 extracts the cell mixture into the culture medium, which will cause excess gas in the pipette barrel 1 after the pipette barrel 1 extracts the cell mixture Instead of fully pumping the cell mixture, the staff can exhaust the excess gas in the pipette gun barrel 1 through the exhaust assembly, and then pass the compression structure to make the pipette gun barrel 1 pump out the excess air into the culture medium again. The volume of cell mixture can extract more cell mixture at one time, which improves the work efficiency.

In some embodiments of the present invention, the compression structure includes a push-pull rod 2 and a compression substrate 7, the push-pull rod 2 runs through the top of the pipette gun barrel 1, one end of the push-pull rod 2 is connected to the compression substrate 7, and the compression substrate 7 is located in the pipette gun. Inside the barrel 1 , and the ring side of the compression substrate 7 is attached to the inner side wall of the pipette barrel 1 .

In this embodiment, the compression structure is composed of a push-pull rod 2 and a compression substrate 7. One end of the push-pull rod 2 is connected to the compression substrate 7. Specifically, firstly, the staff pushes the push-pull rod 2 downward to release the air in the pipette gun barrel 1. discharge, and then pull up the push-pull rod 2 to extract the cell mixture in the culture medium.

In some embodiments of the present invention, an elastic rubber ring is sheathed on the ring side of the compression substrate 7 .

In this embodiment, the ring side of the compression substrate 7 is covered with an elastic rubber ring, which can isolate the air between the compression substrate 7 and the inner wall of the pipette gun barrel 1, and avoid the failure of the work.

In some embodiments of the present invention, a return spring 8 is installed between the compression substrate 7 and the pipette gun barrel 1, one end of the return spring 8 is connected to the compression substrate 7, and the other end of the return spring 8 is connected to the pipette barrel 1 inner upper wall.

In this embodiment, one end of the return spring 8 is connected to the compression substrate 7, and the other end of the return spring 8 is connected to the inner upper wall of the pipette gun barrel 1. After the air is exhausted, the compressed substrate 7 moves upward under the action of the return spring 8 to form a pressure difference between the pipette gun barrel 1 and the outside world, so that the cell mixture can be absorbed.

In some embodiments of the present invention, the exhaust assembly includes a rotating base plate 6 and a first rotating member 5 for rotating the rotating base plate 6, the rotating base plate 6 is rotationally connected with the compression base plate 7, and the compression base plate 7 is provided with a first vent 10 , the rotating base plate 6 is provided with a second vent 9 matching with the first vent 10 , and the top of the pipette barrel 1 is provided with an arc-shaped sliding port 11 for fitting with the first rotating member 5 .

In this embodiment, the exhaust assembly is composed of a rotating base plate 6 and a first rotating member 5, and the rotating base plate 6 and the compression base plate 7 are rotationally connected. Specifically, the staff needs to drain the pipette barrel 1 when extracting the cell mixture. When there is excess gas inside, the staff slides the first rotating member 5 along the direction of the arc-shaped sliding port 11, so that the first vent 10 of the compression substrate 7 is aligned with the second vent 9 of the rotating substrate 6 and closes the block assembly 4, Then the compression base plate 7 is moved downwards so that excess gas is discharged from the first vent 10 , the second vent 9 and the arc-shaped sliding port 11 in sequence.

In some embodiments of the present invention, an elastic rubber ring is sheathed on the ring side of the rotating base plate 6 .

In this embodiment, the ring side of the rotating base 6 is sheathed with an elastic rubber ring to isolate the air between the rotating base 6 and the inner wall of the pipette barrel 1 .

In some embodiments of the present invention, the blocking assembly 4 includes a blocking casing and rollers, the straw body 3 runs through the blocking casing, the blocking casing is provided with rolling grooves that cooperate with the rollers, and both sides of the rolling grooves are provided with The connecting groove, the horizontal height of one end of the connecting groove is higher than the horizontal height of the other end of the connecting groove.

In this embodiment, the blocking assembly 4 is composed of a blocking shell and a roller. When the staff closes the pipette body 3, the roller slides toward the lower end of the connecting groove, so that the roller squeezes the pipette body 3 to prevent the cells in the pipette body 1 from When the mixed liquid flows out, when the staff opens the straw body 3, the roller slides to the high end of the connecting groove, so as to prevent the roller from squeezing the straw body 3, and the work of extracting the cell mixed liquid can be carried out.

In some embodiments of the present invention, an observation window is installed on the side wall of the pipette barrel 1 .

In this embodiment, the staff can observe the situation inside the pipette gun barrel 1 in real time through the observation window, and can take actions at any time.

In some embodiments of the present invention, the observation window is provided with scale lines from top to bottom.

In this embodiment, the worker can clearly know the quantity of the cell mixture in the pipette barrel 1 through the scale line.

Please refer to Figure 4. In some embodiments of the present invention, the pipette gun barrel 1 is composed of a first connecting section 12 and a second connecting section 13, wherein the first connecting section 12 is detachably connected to the second connecting section 13 (the second connecting section 13 The outer wall is provided with threads, the inner wall of the first connecting section 12 is provided with threads, and the second connecting section 13 is connected with the first connecting section 12 through the thread), specifically, after the staff pipetting is completed, the cell solution is installed in the second In the connecting section 13, then relatively rotate the first connecting section 12 and the second connecting section 13 to separate the first connecting section 12 and the second connecting section 13, and then replace another second connecting section 13 for the next pipetting work , since the side walls of the rotating base plate 6 and the compression base plate 7 are covered with elastic rubber, when the second connecting section 13 is connected to the first connecting section 12, the side wall of the second connecting section 13 can be pressed to the first connecting section 12 Between the rotating base plate 6 and the compression base plate 7, the work of pipetting liquid can be carried out smoothly.

To sum up, the embodiment of the present invention provides a cell pipette, including a pipette barrel 1, a straw body 3 and a compression structure; the top of the pipette barrel 1 is installed for compressing the inside of the pipette barrel 1 Compression structure of air; the bottom of the pipette gun barrel 1 is connected to the pipette body 3 that communicates with the inside of the pipette gun barrel 1; Air assembly; the suction pipe body 3 is equipped with a sealing assembly 4 for isolating the inside of the pipette gun barrel 1 from the outside air.

In the above embodiment, a cell pipette consists of a pipette body 1, a pipette body 3 and a compression structure, wherein the upper part of the pipette body 1 is equipped with the compression structure, and the lower part of the pipette body 1 is installed The suction pipe body 3 and the compression structure can be used to compress the air in the pipette gun barrel 1. Specifically, when the staff extracts the cell mixture in the culture medium, the compression structure first compresses the air in the pipette gun barrel 1. The air is discharged, and then the pipette body 3 at the bottom of the pipette gun barrel 1 is placed in the culture solution, and then a negative pressure is generated in the pipette gun barrel 1 through the compression structure, and the pipette gun barrel 1 passes through the pipette body 3 Aspirating the cell mixture into the culture medium, on the one hand, due to the presence of gas in the cell mixture, the pipette barrel 1 draws the cell mixture into the culture medium by using the pressure difference to make the cell mixture enter the pipette barrel 1 Internally, the pressure in the culture solution becomes smaller during the extraction process, and part of the gas in the culture solution is precipitated due to the pressure drop. On the other hand, the pipette gun barrel 1 is too violently drawn into the culture solution, resulting in the culture solution not yet Into the pipette gun barrel 1 in time to generate a short-term vacuum, and then it will disappear. In the third aspect, there is residual gas before the pipette gun barrel 1 extracts the cell mixture into the culture medium. The above problems will cause the staff to remove the cells. After the mixed solution is pumped into the pipette gun barrel 1, there is excess gas in the pipette gun barrel 1, and the above-mentioned excess gas cannot be discharged and then the same volume of cell mixed solution is drawn into the culture medium, resulting in a loss of work efficiency. Low problem, in this application, after the pipette gun barrel 1 pumps the cell mixture in the culture medium into the interior, if there is excess air in the pipette gun barrel 1, the above-mentioned excess air will be discharged to the The outside of the pipette gun barrel 1, during this process, the staff opens the block assembly 4 to prevent the cell mixture in the pipette gun barrel 1 from flowing into the outside from the pipette body 3, when the air in the pipette gun barrel 1 is completely When it is discharged to the outside, the staff opens the compression structure again, so that the pipette gun barrel 1 pumps the same volume of cell mixture as the excess air into the culture medium, so that the pipette gun barrel 1 is filled with the cell mixture, avoiding the transfer After the liquid gun barrel 1 extracts the cell mixture, there is excess air inside, which causes the problem that the working efficiency of the pipette gun barrel 1 is low.

In this embodiment, when the staff draws the cell mixture into the culture medium through the cell pipette gun, the pressure difference of the air is used to draw the cell mixture into the pipette barrel 1. On the one hand, due to the presence of Gas, when the pipette gun barrel 1 draws the cell mixture into the culture medium, the pressure difference is used to make the cell mixture enter the inside of the pipette gun barrel 1, and the pressure in the culture medium becomes smaller during the extraction process, and part of the gas in the culture medium Precipitation due to the problem of pressure drop. Second, the pipette gun barrel 1 is too violently drawn into the culture solution, causing the culture solution to not enter the pipette gun barrel 1 in time to generate a short vacuum, which will then disappear. In the third aspect, there is a problem of residual gas before the pipette barrel 1 extracts the cell mixture into the culture medium, which will cause excess gas in the pipette barrel 1 after the pipette barrel 1 extracts the cell mixture Instead of fully pumping the cell mixture, the staff can exhaust the excess gas in the pipette gun barrel 1 through the exhaust assembly, and then pass the compression structure to make the pipette gun barrel 1 pump out the excess air into the culture medium again. The volume of cell mixture can extract more cell mixture at one time, which improves the work efficiency.

The above are only preferred embodiments of the present invention, and are not intended to limit the present invention. For those skilled in the art, the present invention may have various modifications and changes. Any modifications, equivalent replacements, improvements, etc. made within the spirit and principles of the present invention shall be included within the protection scope of the present invention.

Claims (6)

1. A cell pipette gun, characterized in that, comprising a pipette gun barrel, a suction pipe body and a compression structure; The top of the pipette gun barrel is installed with a compression structure for compressing the air inside the pipette gun barrel; The bottom of the pipette gun barrel is connected to the pipette body that communicates with the interior of the pipette gun barrel; The top of the pipette gun barrel is equipped with an exhaust assembly for exhausting the air inside the pipette gun barrel; The suction pipe body is equipped with a blocking assembly for isolating the pipette barrel from the outside air; The compression structure includes a push-pull rod and a compression substrate, the push-pull rod runs through the top of the pipette barrel, one end of the push-pull rod is connected to the compression substrate, and the compression substrate is located in the pipette barrel , and the ring side of the compression substrate is attached to the inner side wall of the pipette barrel; The exhaust assembly includes a rotating base plate and a first rotating member for rotating the rotating base plate, the rotating base plate is rotatably connected with the compression base plate, the compression base plate is provided with a first vent, and the rotation base plate is provided with There is a second air port cooperating with the first air port, and the top of the pipette gun barrel is provided with an arc-shaped sliding port for cooperating with the first rotating member; The blocking assembly includes a blocking casing and rollers, the straw body runs through the blocking casing, the blocking casing is provided with rolling grooves that match the rollers, and both sides of the rolling grooves are provided with The connecting groove for matching the roller, the level of one end of the connecting groove is higher than the level of the other end of the connecting groove. 2 . The cell pipetting gun according to claim 1 , wherein an elastic rubber ring is sheathed on the ring side of the compression substrate. 3 . 3. A cell pipette gun according to claim 1, wherein a return spring is installed between the compression base plate and the pipette gun barrel, and one end of the return spring is connected to the compression base plate , the other end of the return spring is connected to the inner upper wall of the pipette gun barrel. 4 . The cell pipetting gun according to claim 1 , wherein an elastic rubber ring is sheathed on the ring side of the rotating substrate. 5. A cell pipette gun according to claim 1, characterized in that an observation window is installed on the side wall of the pipette gun barrel. 6. A cell pipette gun according to claim 5, characterized in that, the observation window is provided with scale lines from top to bottom.