CN114076698A

CN114076698A - Piece pushing machine and dye liquor recovery method

Info

Publication number: CN114076698A
Application number: CN202010818400.9A
Authority: CN
Inventors: 谢子贤; 刘隐明
Original assignee: Shenzhen Mindray Bio Medical Electronics Co Ltd
Current assignee: Shenzhen Mindray Bio Medical Electronics Co Ltd
Priority date: 2020-08-14
Filing date: 2020-08-14
Publication date: 2022-02-22

Abstract

The application provides a dye liquor-saving piece pushing machine and a dye liquor recycling method. The sheet pusher includes: the device comprises a sample sucking mechanism for sucking a sample to be detected, a slide loading mechanism for loading a slide, a sample adding mechanism for recording the sample to be detected on the slide, a slide pushing mechanism for flattening the sample to be detected on the slide to prepare a smear, a dye solution mechanism, a dye solution output path, a dye solution recovery path, a conveying mechanism and a control mechanism. The dye liquor mechanism comprises a liquid storage tank and a dyeing box, wherein the liquid storage tank stores dye liquor, and the dyeing box dyes the sample to be detected on the smear. The conveying mechanism conveys the dyeing box from the first dyeing station to the second dyeing station. The control mechanism is configured to: controlling the dye liquor in the liquid storage tank to output the dye liquor to a dyeing box on the first dyeing station through a dye liquor output liquid path, and controlling the conveying mechanism to convey the dyeing box from the first dyeing station to a second dyeing station; and controlling the dye liquor of the dyed sample in the dyeing box on the second dyeing station to be recycled to the liquid storage tank through the dye liquor recycling liquid path.

Description

Piece pushing machine and dye liquor recovery method

Technical Field

The application relates to the technical field of biochemical inspection, in particular to a piece pushing machine and a dye liquor recovery method.

Background

The conventional wafer pushing machine usually requires a dye solution (also referred to as a staining agent) for staining a sample to be measured. The dye liquor has higher cost and larger consumption, which leads to higher use cost of the slice pushing machine.

Disclosure of Invention

The application provides a piece pushing machine capable of saving dye liquor and a dye liquor recycling method.

A blade pusher comprising:

the sample sucking mechanism is used for sucking a sample to be detected;

a slide loading mechanism for loading slides;

the sample adding mechanism is used for loading a sample to be detected to the slide;

the slide pushing mechanism is used for flattening the sample to be detected on the slide to prepare a smear;

the dye solution mechanism comprises a liquid storage tank and a dyeing box, wherein the liquid storage tank is used for storing dye solution, and the dyeing box is used for dyeing the sample to be detected on the smear;

one end of the dye liquor output liquid path is communicated with the liquid storage tank, and the other end of the dye liquor output liquid path is communicated with a dyeing box on the first dyeing station;

one end of the dye liquor recovery liquid path is communicated with the liquid storage tank, and the other end of the dye liquor recovery liquid path is communicated with a dyeing box on a second dyeing station;

a transport mechanism for transporting the staining cassette from the first staining station to the second staining station; and

a control mechanism in communicative connection with the reservoir, the staining cassette, and the transport mechanism, the control mechanism configured to: controlling the dye liquor in the liquid storage tank to output the dye liquor to a dyeing box on the first dyeing station through the dye liquor output liquor path, and controlling the conveying mechanism to convey the dyeing box from the first dyeing station to the second dyeing station; and controlling the dye liquor of the dyed sample to be detected in the dyeing box on the second dyeing station to be recycled to the liquid storage tank through the dye liquor recycling liquid path.

A dye liquor recovery method is applied to a piece pushing machine, the piece pushing machine comprises a sample sucking mechanism, a slide loading mechanism, a sample adding mechanism, a piece pushing mechanism, a dye liquor mechanism and a conveying mechanism, and the dye liquor recovery method comprises the following steps:

a sample sucking step, wherein the sample sucking mechanism sucks a sample to be detected;

a slide loading step of loading a slide by the slide loading mechanism;

loading a sample to be detected onto a slide by using a sample loading mechanism;

a sheet pushing step, in which the sheet pushing mechanism pushes the sample to be detected on the slide flat to make a smear;

the dye solution mechanism comprises a liquid storage tank and a dyeing box, the liquid storage tank is used for storing dye solution, and the dyeing box is used for dyeing the sample to be detected on the smear;

a dye liquor output step, wherein the dye liquor in the liquid storage tank outputs the dye liquor to a dyeing box on a first dyeing station through a dye liquor output liquid path, and the dyeing box receives the dye liquor and dyes a sample to be detected;

a conveying step, wherein the conveying mechanism conveys the dyeing box from a first dyeing station to a second dyeing station; and

and a dye liquor recovery step, namely recovering the dye liquor obtained after the sample to be detected in the dyeing box on the second dyeing station is dyed into the liquid storage tank through a dye liquor recovery liquid path.

Compared with the related art, the dye liquor recovery method can convey the dye liquor in the dyeing box after dyeing the sample to be detected to the second dyeing station, and recover the dye liquor to the liquid storage tank through the dye liquor recovery liquid path, and the dye liquor recovered to the liquid storage tank can be used for dyeing the sample to be detected in the smear again. Therefore, the dye liquor can be recycled by the dye liquor recycling method, the dye liquor is used for dyeing the sample to be tested in the smear for multiple times, and for the smears with the preset number, the required dye liquor is less, so that the use cost of the slide pushing machine is reduced. Further, the dye liquor recovery method of the application uses a liquid storage tank and is matched with a dye liquor output liquid path to output the dye liquor to the dyeing box of the first dyeing station, and is matched with a dye liquor recovery liquid path to recover the dye liquor in the dyeing box conveyed from the first dyeing station to the second dyeing station into the liquid storage tank. Therefore, the number of the liquid storage tanks required in the dye liquor recovery method is small, and the use cost of the sheet pushing machine is further reduced.

Drawings

In order to more clearly illustrate the technical solutions of the embodiments of the present application, the drawings needed to be used in the embodiments will be briefly described below, and it is obvious that the drawings in the following description are some embodiments of the present application, and it is obvious for those skilled in the art to obtain other drawings based on these drawings without creative efforts.

Fig. 1 is a block diagram of a slice pusher according to an embodiment of the present disclosure.

Fig. 2 is a schematic view of a part of the structure of the wafer pusher provided in fig. 1.

Fig. 3 is a block diagram of a chip pusher according to another embodiment of the present application.

Fig. 4 is a block diagram of a slice pusher according to another embodiment of the present application.

Fig. 5 is a block diagram of a chip pusher according to another embodiment of the present application.

Fig. 6 is a block diagram of a chip pusher according to still another embodiment of the present application.

Fig. 7 is a block diagram of a push slice machine according to another embodiment of the present application.

Fig. 8 is a flowchart of a dye liquor recovery method according to an embodiment of the present application.

FIG. 9 is a flow chart of a dye liquor recovery method according to yet another embodiment of the present application.

FIG. 10 is a flow chart of a dye liquor recovery method according to another embodiment of the present application.

FIG. 11 is a flow chart of a dye liquor recovery method according to another embodiment of the present application.

FIG. 12 is a flow chart of a dye liquor recovery method according to another embodiment of the present application.

FIG. 13 is a flow chart of a dye liquor recovery method according to another embodiment of the present application.

FIG. 14 is a flow chart of a dye liquor recovery method according to another embodiment of the present application.

FIG. 15 is a flow chart of a dye liquor recovery method according to yet another embodiment of the present application.

Fig. 16 is a flowchart of detecting the amount of dye solution in the reservoir in the dye solution collecting method according to the embodiment of the present application.

Fig. 17 is a flowchart of detecting the amount of dye liquor in a tank in a dye liquor recovery method according to another embodiment of the present application.

FIG. 18 is a flow chart of a dye liquor recovery method according to yet another embodiment of the present application.

FIG. 19 is a flow chart of a dye liquor recovery method according to yet another embodiment of the present application.

Detailed Description

The present invention will be described in further detail with reference to the following detailed description and accompanying drawings. Wherein like elements in different embodiments are numbered with like associated elements. In the following description, numerous details are set forth in order to provide a better understanding of the present application. However, those skilled in the art will readily recognize that some of the features may be omitted or replaced with other elements, materials, methods in different instances. In some instances, certain operations related to the present application have not been shown or described in detail in order to avoid obscuring the core of the present application from excessive description, and it is not necessary for those skilled in the art to describe these operations in detail, so that they may be fully understood from the description in the specification and the general knowledge in the art.

Furthermore, the features, operations, or characteristics described in the specification may be combined in any suitable manner to form various embodiments. Also, the various steps or actions in the method descriptions may be transposed or transposed in order, as will be apparent to one of ordinary skill in the art. Thus, the various sequences in the specification and drawings are for the purpose of describing certain embodiments only and are not intended to imply a required sequence unless otherwise indicated where such sequence must be followed.

The numbering of the components as such, e.g., "first", "second", etc., is used herein only to distinguish the objects as described, and does not have any sequential or technical meaning. The term "connected" and "coupled" when used in this application, unless otherwise indicated, includes both direct and indirect connections (couplings).

Referring to fig. 1 and fig. 2 together, fig. 1 is a block diagram of a wafer pushing machine according to an embodiment of the present disclosure; fig. 2 is a schematic view of a part of the structure of the wafer pusher provided in fig. 1. The slide pusher 1 comprises a sample sucking mechanism 110, a slide loading mechanism 120, a sample adding mechanism 130, a slide pushing mechanism 140, a dye solution mechanism 150, a dye solution output liquid path 160, a dye solution recovery liquid path 170, a conveying mechanism 180 and a control mechanism 190. The sample sucking mechanism 110 is used for sucking a sample to be measured. The slide loading mechanism 120 is used to load slides. The loading mechanism 130 is used to load a sample to be tested onto a slide. The slide pushing mechanism 140 is used for flattening the sample to be tested on the slide to make a smear. The staining solution mechanism 150 comprises a liquid storage tank 151 and a staining box 152, wherein the liquid storage tank 151 is used for storing the staining solution, and the staining box 152 is used for staining the sample to be detected on the smear. One end of the dye liquor output liquor path 160 is communicated with the liquor storage tank 151, and the other end is communicated with the dyeing box 152 on the first dyeing station 11. One end of the dye liquor recovery liquid path 170 is communicated with the liquid storage tank 151, and the other end is communicated with the dyeing box 152 on the second dyeing station 12. The conveying mechanism 180 is used for conveying the dyeing box 152 from the first dyeing station 11 to the second dyeing station 12. A control mechanism 190 communicatively coupled to the reservoir 151, the staining cassette 152, and the transport mechanism 180, the control mechanism 190 configured to: controlling the dye liquor in the liquid storage tank 151 to output the dye liquor to the dyeing box 152 on the first dyeing station 11 through the dye liquor output liquor path 160, and controlling the conveying mechanism 180 to convey the dyeing box 152 from the first dyeing station 11 to the second dyeing station 12; and controlling the dye liquor obtained after the sample to be detected in the dyeing box 152 on the second dyeing station 12 is dyed to be recycled into the liquid storage tank 151 through the dye liquor recycling liquor path 170.

The sample to be tested includes, but is not limited to, microorganisms, blood, body fluid, bone marrow fluid and other samples. In an embodiment, when the sample sucking mechanism 110 sucks a sample to be detected, the sample to be detected is mixed uniformly, so as to improve a smear prepared according to the sample to be detected, and detect the detection precision of the smear. The sample aspirating mechanism 110 can include, but is not limited to, a sampling device and other components associated with the sampling device, such as a transfer tube. The sampling device may be, but is not limited to, a sampling needle. When a sampling device in the sample suction mechanism 110 sucks a sample to be detected, the sample suction mechanism is divided into puncture sample suction and open sample suction according to different containers for containing the sample to be detected. When the container for containing the sample to be tested is a container with a cover, the sampling device needs to penetrate through the cover of the container when sucking the sample to be tested, and therefore, the mode of sucking the sample to be tested is called puncture sample sucking. When the opening of the container for containing the sample to be tested is open and the cover is not provided, the sampling device directly sucks the sample from the open opening of the container when sucking the sample to be tested, and therefore, the mode of sucking the sample to be tested is called open sample sucking.

The slide loading mechanism 120 is used for loading a slide, and the loading mechanism 130 is used for loading a sample to be tested on the slide. In one embodiment, the sample adding mechanism 130 is in communication with the sample sucking mechanism 110 via a pipe, the sample to be tested sucked by the sample sucking mechanism 110 reaches the sample adding mechanism 130 via the pipe, and the sample to be tested is loaded onto the slide by the sample adding mechanism 130 by means of drip or the like. In one embodiment, the height of the sample adding mechanism 130 is lower than that of the sample sucking mechanism 110, so that the sample to be tested sucked by the sample sucking mechanism 110 can flow into the sample adding mechanism 130 from the pipeline more easily.

The specimen to be tested is usually concentrated on a certain area on the slide, for example, in the form of a droplet, when it is loaded on the slide. The slide pushing mechanism 140 smoothes the sample to be tested on the slide, and the formed smear is usually film-shaped. If the sample to be tested is to be developed into a smear with the external dimension (area, width, length and external shape) meeting the requirement of the distribution of cells under the microscope, the volume (namely the amount of the sample to be tested), the blade pushing speed and the blade pushing angle of the sample to be tested must be reasonably selected. Generally, the larger the volume of the sample to be detected is, the thicker the thickness of the formed smear is, and the longer the smear is under the condition of a certain width; the higher the blade pushing speed is, the thicker the thickness of the formed smear is, and the shorter the length of the smear is; the larger the angle of the push plate, the thicker the thickness and the shorter the length. In addition, it should be noted that under the same parameters of the blade (such as blade pushing speed and blade pushing angle), various external factors, such as ambient temperature, ambient humidity, the time for the sample to be tested to be placed, and the storage condition of the sample to be tested, all affect the form of the formed smear. For example, for the same sample to be tested, under the same push-piece parameters: the lower the temperature, the thicker the thickness of the smear formed; the higher the temperature, the thinner the thickness of the smear formed and the longer the length of the smear.

The liquid storage tank 151 is used for storing dye liquor, and the smear can be dyed by the dye liquor. The type of the staining solution is different according to the items for detecting the smear. One end of the dye liquor output liquor path 160 is communicated with the liquor storage tank 151, and the other end is communicated with the dyeing box 152 on the first dyeing station 11. It can be seen that the dye solution stored in the reservoir 151 can be output to the dyeing box 152 at the first dyeing station 11 through the dye solution output path 160. The dye liquor output circuit 160 may include, but is not limited to including, a pipe, etc. When the smear needs to be dyed, the liquid storage tank 151 outputs the dye solution to the dyeing box 152 on the first dyeing station 11 through the dye solution output path 160 under the control of the control mechanism 190. The staining cassette 152 stains the sample to be tested on the smear. After the staining box 152 stains the sample to be tested on the smear, the transporting mechanism 180 transports the staining box 152 down from the first staining station 11 to the second staining station 12 under the control of the control mechanism 190. In one embodiment, the first dyeing station 11 is spaced apart from the second dyeing station 12. One end of the dye liquor recovery liquid path 170 is communicated with the liquid storage tank 151, and the other end is communicated with the dyeing box 152 on the second dyeing station 12. It can be seen that the dye liquor in the dye box 152 at the second dyeing station 12 can be recovered into the liquid storage tank 151 through the dye liquor recovery liquid path 170. After the dyeing box 152 is transferred to the second dyeing station 12, the control mechanism 190 controls the dyed dyeing solution of the sample to be tested in the dyeing box 152 on the second dyeing station 12 to be recycled to the liquid storage tank 151 through the liquid path.

It should be noted that the control mechanism 190 may include, but is not limited to including, a processor. The conveying mechanism 180 may be, but is not limited to, a conveyor belt or the like.

Compared with the related art, the control mechanism 190 of the slide loader 1 of the present application can control the transport mechanism 180 to transport the dye solution in the dye box 152 after dyeing the sample to be tested to the second dyeing station 12, and recycle the dye solution to the liquid storage tank 151 through the dye solution recycling liquid path 170, and the dye solution recycled to the liquid storage tank 151 can be used again to dye the sample to be tested in the smear. Therefore, the film pushing machine 1 can recycle the dye liquor, and is used for dyeing the samples to be tested in the smears for multiple times, and the required dye liquor is less for the smears with the preset number, so that the use cost of the film pushing machine 1 is reduced. Further, the slide loader 1 of the present application uses one liquid reservoir 151 and cooperates with the dye liquor output liquid path 160 to output the dye liquor to the dyeing box 152 of the first dyeing station 11, and cooperates with the dye liquor recovery liquid path 170 to recover the dye liquor in the dyeing box 152 transported from the first dyeing station 11 to the second dyeing station 12 into the liquid reservoir 151. Therefore, the number of the liquid storage tanks 151 required in the wafer pushing machine 1 of the present application is small, and the use cost of the wafer pushing machine 1 is further reduced.

Generally, after the slide pushing mechanism 140 smoothes the sample to be tested on the slide, the smoothed sample to be tested may be dried to stabilize the form of the smoothed sample to be tested. Specifically, referring to fig. 3, fig. 3 is a block diagram of a slice pusher according to another embodiment of the present application. The sheet pusher 1 further comprises a drying mechanism 220, wherein the drying mechanism 220 is used for drying and detecting the dried sample to be detected so as to judge the drying degree of the sample to be detected. Accordingly, the control mechanism 190 is configured for: and when the drying degree of the sample to be detected is greater than or equal to the preset drying degree, controlling the dyeing box 152 to dye the sample to be detected. And when the drying degree of the sample to be tested is smaller than the preset drying degree, controlling the drying mechanism 220 to continuously dry the sample to be tested.

In some embodiments, the flattened and dried sample to be tested may be further subjected to unfolding detection to determine whether the sample to be tested is unfolded and whether the unfolded state meets the requirements. Specifically, referring to fig. 4, fig. 4 is a block diagram of a slice pushing machine according to another embodiment of the present application. The blade pushing mechanism 140 further includes a detection mechanism 230, and the detection mechanism 230 is configured to perform unfolding detection on the flattened and dried sample to be detected, so as to determine whether the sample to be detected is unfolded and whether the unfolded state meets the requirement. Accordingly, the control mechanism 190 is configured for: when the unfolded state of the sample to be tested meets the requirement, the staining box 152 is controlled to stain the sample to be tested. When the unfolded state of the sample to be detected does not meet the requirement, the slide pushing mechanism 140 is controlled to continue to level the sample to be detected on the slide. Or when the unfolded state of the sample to be detected does not meet the requirement, prompting that the sample to be detected does not meet the requirement so as to discard the sample to be detected. It is understood that, in other embodiments, the sheet pusher 1 may not include the drying mechanism 220, may not include the detecting mechanism 230, or may include only one of the drying mechanism 220 and the detecting mechanism 230, or may include both of the drying mechanism 220 and the detecting mechanism 230.

In one embodiment, the control mechanism 190 is further configured to: and judging whether the times of dye liquor recovery reach preset times or not, and controlling the dyed dye liquor in the dyeing box 152 on the second dyeing station 12 to be discharged through a waste liquor channel when the times of dye liquor recovery reach the preset times.

For each working cycle of the blade pusher 1, one dye liquor output and dye liquor recovery operation is required. When the use cycle of the dye liquor reaches a certain number of times, the performance of the dye liquor is reduced, and if the dye liquor is used for dyeing the sample to be detected in the smear, the dyeing effect is poor, and the subsequent detection of the dyed sample to be detected is not facilitated. The utility model provides a control mechanism 190 is configured and is used for judging whether the number of times that the dye liquor was retrieved reaches and predetermines the number of times, when the number of times that the dye liquor was retrieved reached and predetermines the number of times, controls the dye liquor after dyeing in the dyeing box 152 on the second dyeing station 12 passes through the waste liquid passageway and discharges. As can be understood, when the number of times of recovering the dye solution does not reach the preset number of times, the controller continues to control the dye solution after dyeing in the dyeing box 152 at the second dyeing station 12 to be recovered into the liquid storage tank 151 through the dye solution recovering solution path 170.

The preset number may be input by a user through an input device (e.g., a keyboard, a touch display panel, etc.) of the slide loader 1, or may be automatically set by a system of the slide loader 1. For example, the predetermined number of times may be, but is not limited to, 50 times.

Further, the control mechanism 190 is further configured to: and judging whether the amount of the dye liquor in the liquid storage tank 151 reaches a first set value or not, and controlling the dye liquor in the liquid storage tank 151 to be discharged through a waste liquor channel when the amount of the dye liquor in the liquid storage tank 151 reaches the first set value.

Since the dyed dye solution in the dyeing box 152 on the second dyeing station 12 is discharged through the waste liquid channel when the number of times of dye solution recovery reaches the preset number of times, then the dye solution in the liquid storage tank 151 gradually decreases as the process of dyeing the sample to be tested of the smear proceeds. When the amount of the dye solution in the liquid storage tank 151 obtains a first set value, the control mechanism 190 can control the dye solution in the liquid storage tank 151 to be discharged through a waste liquid channel. When the amount of the dye solution in the liquid storage tank 151 obtains the first set value, it indicates that the amount of the dye solution in the liquid storage tank 151 is small and cannot meet the amount required for dyeing the smear sample to be detected, or the amount required for dyeing the smear sample to be detected is met but cannot support dyeing for a large number of times, and then the dye solution in the liquid storage tank 151 is removed through the waste liquid channel to prepare for inputting a new dye solution into the liquid storage tank 151 again.

In one embodiment, the control mechanism 190 is further configured to: and judging whether the dye solution in the liquid storage tank 151 is emptied, and inputting new dye solution into the liquid storage tank 151 again when the dye solution in the liquid storage tank 151 is emptied.

Since the liquid storage tank 151 has a large volume and the amount of dye solution sucked in each work cycle is limited, when new dye solution is input into the liquid storage tank 151 again, the liquid storage tank 151 is not filled with the dye solution at one time, but new dye solution is input into the liquid storage tank 151 in multiple cycles until the amount of the input new dye solution reaches a set value (a second set value). It will be appreciated that in other embodiments, the reservoir 151 is filled with a new dye solution at a time.

In one embodiment, the control mechanism 190 is further configured to: and judging whether the amount of the newly input dye liquor in the liquid storage tank 151 reaches a second set value, and controlling the newly input dye liquor in the liquid storage tank 151 to be output to the dyeing box 152 on the first dyeing station 11 through the dye liquor output liquid path 160 when the amount of the newly input dye liquor in the liquid storage tank 151 reaches the second set value.

When the amount of the newly input dye solution in the liquid storage tank 151 reaches a second set value, the newly input dye solution in the liquid storage tank 151 can be considered to meet the requirement of dyeing the sample to be detected on the smear in the dyeing box 152 on the first dyeing station 11 for one or more times.

In one embodiment, the control mechanism 190 controls the time during and after the process of outputting the newly input dye solution in the liquid storage tank 151 to the dyeing box 152 at the first dyeing station 11 through the dye solution output path 160, and the control mechanism 190 also controls the liquid storage tank 151 to input new dye solution until the liquid storage tank 151 is filled. The liquid reservoir 151 of the blade pusher 1 in the present embodiment does not need to be stopped to replace the dye solution before the blade pusher 1 is filled, and thus time can be saved.

Further, the control mechanism 190 is further configured to: and judging whether the newly input amount of the dye solution in the liquid storage tank 151 reaches a third set value, and stopping inputting the new dye solution into the liquid storage tank 151 when the newly input amount of the dye solution in the liquid storage tank 151 reaches the third set value.

The third set value is greater than the second set value. When the newly input amount of dye liquid in the reservoir 151 reaches a third set value, it may be considered that the reservoir 151 is already filled or substantially filled. At this time, the input of new dye solution into the tank 151 is stopped so as to prevent the new dye solution input into the tank 151 from overflowing from the tank 151. In one embodiment, after the reservoir 151 is filled, the dye liquor is completely renewed and a new cycle (i.e., dye liquor output and dye liquor recovery) is started.

The principle of the blade pusher 1 measuring the amount of the dye solution in the reservoir 151 in one embodiment will be described in detail below. Referring to fig. 5, fig. 5 is a block diagram of a chip pushing machine according to another embodiment of the present application. The blade pushing machine 1 further comprises a double liquid level sensor 240. The dual level sensor 240 is used to detect the lower limit value and the upper limit value of the dye solution in the liquid storage tank 151. The control mechanism 190 is electrically connected to the dual level sensor 240. The control mechanism 190 is further configured to: comparing the lower limit value of the dye liquor with the first set value to judge whether the dye liquor in the liquid storage tank 151 reaches the first set value, and comparing the upper limit value of the dye liquor with the third set value to judge whether the dye liquor in the liquid storage tank 151 reaches the third set value.

The blade pusher 1 including the dual liquid level sensor 240 may be incorporated into the blade pusher 1 according to any of the foregoing embodiments, and in the schematic diagram of the present embodiment, the blade pusher 1 including the dual liquid level sensor 240 is incorporated into the blade pusher 1 according to fig. 1 and its related embodiments.

The principle of the blade pusher 1 measuring the amount of the dye solution in the reservoir 151 in one embodiment will be described in detail below. Referring to fig. 6, fig. 6 is a block diagram of a chip pusher according to still another embodiment of the present application. The blade pushing machine 1 further comprises a single liquid level sensor 250 arranged in the liquid storage tank 151. The control mechanism 190 is electrically connected to the single level sensor 250, the control mechanism 190 further configured to: receiving the signal output by the single liquid level sensor 250, calculating the liquid level of the dye liquid in the liquid storage tank 151 according to the signal to obtain a detection value, comparing the detection value with the first set value and the third set value to judge whether the liquid level in the liquid storage tank 151 reaches the first set value, and judging whether the liquid level in the liquid storage tank 151 reaches the third set value.

The blade pusher 1 including the single level sensor 250 may be incorporated into the blade pusher 1 according to any embodiment except for the embodiment including the dual level sensor 240, and in the schematic view of the present embodiment, the blade pusher 1 including the single level sensor 250 is incorporated into the blade pusher 1 according to fig. 1 and the related embodiment.

In one embodiment, the control mechanism 190 is further configured to: judging whether the dye liquor in the dyeing box 152 is newly input dye liquor or dye liquor with the recovery times reaching preset times; when the dye liquor in the dye liquor box reaches the preset times, controlling the dye liquor in the dye liquor box on the second dyeing station 12 to be discharged through a waste liquor channel; when the dye solution in the dyeing box 152 is a newly input dye solution, the newly input dye solution obtained by dyeing the sample to be tested in the dyeing box 152 at the second dyeing station 12 is controlled to be recycled to the liquid storage tank 151 through the dye solution recycling liquid path 170.

In an embodiment, please refer to fig. 7, and fig. 7 is a block diagram of a slice pusher according to another embodiment of the present application. The wafer pusher 1 further comprises a pressure control module 210. The pressure control module 210 is configured to adjust the pressure of the liquid storage tank 151, when the liquid storage tank 151 is at positive pressure, the liquid storage tank 151 outputs the dye solution to the dyeing box 152 through the dye solution output path 160, and when the liquid storage tank 151 is at negative pressure, the dye solution after the dyeing of the sample to be tested is recovered to the liquid storage tank 151 through the dye solution recovery path 170. The blade pushing machine 1 further includes a pressure control module 210, which can be incorporated into the blade pushing machine 1 provided in any of the foregoing embodiments, and in this embodiment, the blade pushing machine 1 including the pressure control module 210 is incorporated into the blade pushing machine 1 shown in fig. 1 and the related embodiments thereof as an example.

In one embodiment, the pressure control module 210 may adjust the pressure in the reservoir 151 to a positive pressure and a negative pressure. Specifically, in one embodiment, the pressure control module 210 may only be capable of adjusting the pressure in the reservoir 151 to a positive pressure and a negative pressure, and in another embodiment, the pressure control module 210 may be capable of adjusting the pressure in the reservoir 151 to a positive pressure, a negative pressure, and a normal pressure. When the reservoir 151 is at a positive pressure, the pressure within the reservoir 151 is greater than atmospheric pressure; at this time, the liquid reservoir 151 outputs the dye solution to the dyeing box 152 through the dye solution output path 160 by the pressure difference between the liquid reservoir 151 and the dyeing box 152 at the first dyeing station 11. When the reservoir 151 is at a negative pressure, the pressure of the reservoir 151 is less than atmospheric pressure; at this time, by the pressure difference between the liquid storage tank 151 and the dyeing box 152 on the second dyeing station 12, the dye solution obtained by dyeing the sample to be tested is recovered to the liquid storage tank 151 through the dye solution recovery liquid path 170.

In one embodiment, the pressure control module 210 may adjust the pressure in the reservoir 151 to a normal pressure and a negative pressure. The positive pressure in the reservoir 151 needs to be adjusted by means of an additional power module.

The pressure control module 210 may be, but is not limited to, connected to the reservoir 151 through a gas valve to adjust the pressure in the reservoir 151.

Referring to fig. 2 again, the sheet pushing machine 1 further includes an air valve 1, an air valve 2, a liquid valve 1, a liquid valve 2, and a liquid valve 3. The air valve 1 and the air valve 2 are both connected to the liquid storage tank 151 and used for adjusting the air pressure of the liquid storage tank 151. The liquid valve 1 has two states of closing and opening, when the liquid valve 1 is in the opening state, the dye liquor can enter the liquid storage tank 151 through the liquid valve 1 by a pipeline connected with the liquid valve 1 under the matching of the air valve 1 and the air valve 2, and when the liquid valve 1 is in the closing state, the dye liquor can not enter the liquid storage tank 151 through the liquid valve 1 and the pipeline connected with the liquid valve 1. The dye liquor output circuit 160 includes a quantitative module 161 and a pipeline connected between the quantitative module 160 and the liquid storage tank 151. The quantitative module 161 is used for sucking a preset volume of dye solution from the dye solution output path 160. The liquid valve 2 has two states of closing and opening, and when the liquid valve 2 is in the opening state, the dyeing liquid obtained after dyeing the sample to be tested in the dyeing box 152 on the second dyeing station 12 can be recovered to the liquid storage tank 151 through the liquid valve 2 and a pipeline connected with the liquid valve under the cooperation of the air valve 1 and the air valve 2. In other words, the dye liquor recovery liquid path 170 includes a liquid valve 2 and a pipeline communicating with the liquid valve 2. When the liquid valve 3 is in a closed state, the dye solution dyed by the sample to be tested in the dyeing box 152 on the second dyeing station 12 cannot be recycled to the liquid storage tank 151 through the liquid valve 2 and the pipeline connected with the liquid valve. As can be seen, the dye liquor recovery liquid path 170 includes the liquid valve 2 and a pipeline connected to the liquid valve 2. The liquid valve 3 has two states of closing and opening, and when the liquid valve 3 is in the opening state, the dye liquid in the liquid storage tank 151 can be discharged; when the liquid valve 3 is in a closed state, the dye liquid in the reservoir 151 cannot be discharged.

The application also provides a dye liquor recovery method, the dye liquor recovery method is applied to the sheet pushing machine 1, and the sheet pushing machine 1 can achieve the dye liquor recovery method. Referring to fig. 8, fig. 8 is a flowchart illustrating a dye liquor recycling method according to an embodiment of the present disclosure. The method for recovering the dye solution is applied to the slide pusher 1 according to any of the embodiments, and the slide pusher 1 includes a sample suction mechanism 110, a slide loading mechanism 120, a sample adding mechanism 130, a slide pushing mechanism 140, a dye solution mechanism 150, and a conveying mechanism 180. The staining solution mechanism 150 comprises a liquid storage tank 151 and a staining box 152, wherein the liquid storage tank 151 is used for storing the staining solution, and the staining box 152 is used for staining the sample to be detected on the smear. Please refer to the foregoing description for each mechanism in the blade pusher 1, which is not described herein again. The dye liquor recovery method includes, but is not limited to, S110, S120, S130, S140, S150, S160, and S170, and the details of S110, S120, S130, S140, S150, S160, and S170 are described below.

S110, a sample sucking step, wherein the sample sucking mechanism 110 sucks a sample to be detected.

S120, a slide loading step, the slide loading mechanism 120 loading slides.

S130, loading the sample to be tested on the slide by the sample loading mechanism 130.

S140, a sheet pushing step, wherein the sheet pushing mechanism 140 pushes the sample to be detected on the slide flat to make a smear.

S150, a step of outputting a dye solution, in which the dye solution in the liquid storage tank 151 outputs the dye solution to a dyeing box 152 on the first dyeing station 11 through a dye solution output liquid path 160, the dyeing box 152 receives the dye solution and dyes the sample to be detected, wherein one end of the dye solution output liquid path 160 is communicated with the liquid storage tank 151, and the other end is communicated with the dyeing box 152 on the first dyeing station 11.

S160, a conveying step, in which the conveying mechanism 180 conveys the dyeing box 152 from the first dyeing station 11 to the second dyeing station 12.

And S170, a dye liquor recycling step, wherein the dye liquor of the sample to be detected in the dyeing box 152 on the second dyeing station 12 after dyeing is recycled into the liquid storage tank 151 through a dye liquor recycling liquid path 170, wherein one end of the dye liquor recycling liquid path 170 is communicated with the liquid storage tank 151, and the other end of the dye liquor recycling liquid path is communicated with the dyeing box 152 on the second dyeing station 12.

Compared with the related art, the dye liquor recovery method of the present application can transport the dye liquor obtained after dyeing the sample to be tested in the dyeing box 152 to the second dyeing station 12, and recover the dye liquor into the liquid storage tank 151 through the dye liquor recovery liquid path 170, and the dye liquor recovered into the liquid storage tank 151 can be used for dyeing the sample to be tested in the smear again. Therefore, the dye liquor can be recycled by the dye liquor recycling method, the dye liquor is used for dyeing the sample to be tested in the smear for multiple times, and for the smears with the preset number, the dye liquor needed by the method is less, so that the use cost of the sheet pushing machine 1 is reduced. Further, the dye liquor recovery method of the present application uses a liquid storage tank 151 and cooperates with the dye liquor output liquid path 160 to output the dye liquor to the dyeing box 152 of the first dyeing station 11, and cooperates with the dye liquor recovery liquid path 170 to recover the dye liquor in the dyeing box 152 conveyed from the first dyeing station 11 to the second dyeing station 12 into the liquid storage tank 151. Therefore, the number of the liquid storage tanks 151 required in the dye liquor recovery method is small, and the use cost of the sheet pusher 1 is further reduced.

Referring to fig. 9, fig. 9 is a flowchart of a dye liquor recycling method according to another embodiment of the present application. The sheet pusher 1 further comprises a drying mechanism 220, wherein the drying mechanism 220 is used for drying and detecting the dried sample to be detected so as to judge the drying degree of the sample to be detected.

Correspondingly, the dye liquor recovery method also comprises S10, S30, S10 and S30 which are described in detail as follows. S10, and S30 may be located between S140 and S150.

And S10, performing drying detection on the dried sample to be detected to judge the drying degree of the sample to be detected.

And when the drying degree of the sample to be detected is greater than or equal to the preset drying degree, the step S150 is performed.

And S30, when the drying degree of the sample to be tested is smaller than the preset drying degree, controlling the drying mechanism 220 to continuously dry the sample to be tested.

Referring to fig. 10, fig. 10 is a flowchart illustrating a dye liquor recycling method according to another embodiment of the present application. The sheet pusher 1 further comprises a detection mechanism 230, wherein the detection mechanism 230 is used for performing unfolding detection on the flattened and dried sample to be detected so as to judge whether the sample to be detected is unfolded and whether the unfolded state meets the requirement. Correspondingly, the dye liquor recovery method also comprises S40 and S60. S40 and S60 are described in detail below. In this embodiment, S40 may be located between S140 and S150.

S40, detecting the unfolded state of the sample to be detected to judge whether the unfolded state of the sample to be detected meets the requirement.

When the unfolded state of the sample to be detected meets the requirement, the step S150 is carried out; when the unfolded state of the sample to be tested does not meet the requirement, the procedure goes to step S60.

S60, controlling the slide pusher 1 to continue to trowel the sample to be detected on the slide; or prompting that the sample to be detected does not meet the requirement so as to discard the sample to be detected.

It is understood that, in other embodiments, the sheet pusher 1 may not include the drying mechanism 220, may not include the detecting mechanism 230, or may include only one of the drying mechanism 220 and the detecting mechanism 230, or may include both of the drying mechanism 220 and the detecting mechanism 230. Accordingly, when the sheet pusher 1 does not include the drying mechanism 220, the dye liquor recovery method does not include steps (S10, and S30) corresponding to the drying mechanism 220; when the slide loader 1 does not include the detection mechanism 230, the dye liquor recovery method does not include the steps corresponding to the detection mechanism 230 (S40, and S60). When the sheet pusher 1 includes the drying mechanism 220 and the detecting mechanism 230, the dye liquor recovery method may include a step corresponding to the drying mechanism 220 and a step corresponding to the detecting mechanism 230 at the same time.

Referring to fig. 11, fig. 11 is a flowchart illustrating a dye liquor recycling method according to another embodiment of the present application. The dye liquor recovery method also comprises S180 and S190, and the details of S180 and S190 are described as follows. The dye liquor recovery method further comprises a step S180 and a step S190, which can be combined with the dye liquor recovery method provided in any of the foregoing embodiments, and in the schematic diagram of the embodiment, the dye liquor recovery method comprising the step S180 and the step S190 is combined with the dye liquor recovery method described in fig. 8 and the related embodiments thereof for illustration.

S180, a first judgment step, namely judging whether the times of dye liquor recovery reach preset times.

And S190, a discharging step, namely controlling the dyed dye liquor in the dyeing box 152 on the second dyeing station 12 to be discharged through a waste liquor channel when the times of dye liquor recovery reach the preset times.

For each working cycle of the blade pusher 1, one dye liquor output and dye liquor recovery operation is required. When the use cycle of the dye liquor reaches a certain number of times, the performance of the dye liquor is reduced, and if the dye liquor is used for dyeing the sample to be detected in the smear, the dyeing effect is poor, and the subsequent detection of the dyed sample to be detected is not facilitated. The utility model provides a control mechanism 190 is configured and is used for judging whether the number of times that the dye liquor was retrieved reaches and predetermines the number of times, when the number of times that the dye liquor was retrieved reached and predetermines the number of times, controls the dye liquor after dyeing in the dyeing box 152 on the second dyeing station 12 passes through the waste liquid passageway and discharges. As can be understood, when the number of times of dye liquor recovery does not reach the preset number of times, the dye liquor recovery method comprises the following steps: s200, controlling the dyed dye solution in the dyeing box 152 on the second dyeing station 12 to be recovered to the liquid storage tank 151 through the dye solution recovery liquid path 170.

Referring to fig. 12, fig. 12 is a flowchart illustrating a dye liquor recycling method according to another embodiment of the present application. The dye liquor recovery method further comprises S210 and S220, and the details of S210 and S220 are described below.

S210, a second judging step of judging whether the amount of the dye solution in the liquid storage tank 151 reaches a first set value.

And S220, a discharging step, wherein when the amount of the dye liquor in the liquid storage tank 151 reaches a first set value, the dye liquor in the liquid storage tank 151 is controlled to be discharged through the waste liquor channel.

It is understood that the fact that the quantity of dye liquor in said tank 151 reaches a first set value means that the quantity of dye liquor in said tank 151 is equal to the first set value.

When the amount of the dye solution in the reservoir 151 is greater than the first set value, step S200 is performed.

Referring to fig. 13, fig. 13 is a flowchart illustrating a dye liquor recycling method according to another embodiment of the present application. After the discharging step, the dye liquor recycling method further includes S230 and S240, and the details of the S230 and S240 are described below.

S230, a third judging step of judging whether the dye solution in the solution reservoir 151 is emptied.

S240, a filling step, when the reservoir 151 is emptied, a new dye solution is re-input into the reservoir 151.

It is understood that when the dye solution in the reservoir 151 is not drained, the dye solution in the reservoir 151 is continuously drained, i.e., S220 is continuously performed.

Referring to fig. 14, fig. 14 is a flowchart illustrating a dye liquor recycling method according to another embodiment of the present application. After the filling step, the dye liquor recovery method further includes steps S250, and S260, and S250 and S260 are described in detail below.

S250, a fourth judgment step of judging whether the amount of the dye liquor newly input into the liquid storage tank 151 reaches a second set value.

And S260, a dye liquor re-output step, namely controlling the newly input dye liquor in the liquid storage tank 151 to be output to a dye liquor box on the first dyeing station 11 through the dye liquor output liquid path 160 when the amount of the newly input dye liquor in the liquid storage tank 151 reaches a second set value.

It is understood that when the amount of the newly input dye solution in the reservoir 151 does not reach the second set value, the process proceeds to S240.

In one embodiment, the dye liquor recycling method controls the time during and after the process that the newly input dye liquor in the liquid storage tank 151 is output to the dyeing box 152 on the first dyeing station 11 through the dye liquor output liquid path 160, and the control mechanism 190 also controls the liquid storage tank 151 to input new dye liquor until the liquid storage tank 151 is filled. In the dye liquor recovery method according to the present embodiment, the liquid tank 151 does not need to be stopped and the dye liquor is replaced before the blade pusher 1 is filled, and thus time can be saved.

Please refer to fig. 15, fig. 15 is a flowchart illustrating a dye liquor recycling method according to still another embodiment of the present application. After the step of re-outputting the dye liquor, the method for recycling the dye liquor further comprises S270 and S280, and the details of S270 and S280 are described as follows.

S270, a fifth judgment step of judging whether the amount of the dye liquor newly input into the liquid storage tank 151 reaches a third set value.

S280, stopping the filling step, and stopping inputting a new dye solution into the liquid storage tank 151 when the amount of the newly input dye solution in the liquid storage tank 151 reaches a third set value.

It is understood that when the amount of the newly input dye solution in the reservoir 151 does not reach the third set value, the process proceeds to S240.

Referring to fig. 16, fig. 16 is a flowchart for detecting the amount of the dye solution in the solution storage tank in the dye solution recycling method according to an embodiment of the present application. The blade pusher 1 comprises a double liquid level sensor 240. The dual level sensor 240 is used to detect the lower limit value and the upper limit value of the dye solution in the liquid storage tank 151. The control mechanism 190 is electrically connected to the dual level sensor 240. The detection method comprises the following steps of S1 and S1.

S1, comparing the lower limit value of the dye liquor with the first set value to determine whether the dye liquor in the liquor storage tank 151 reaches the first set value, and comparing the upper limit value of the dye liquor with the third set value to determine whether the dye liquor in the liquor storage tank 151 reaches the third set value.

Referring to fig. 17, fig. 17 is a flowchart illustrating a method for detecting an amount of dye liquor in a liquor storage tank according to another embodiment of the present application. The blade pushing machine 1 further comprises a single liquid level sensor 250 arranged in the liquid storage tank 151. The control mechanism 190 is electrically connected to the single level sensor 250. The detection methods include S2 and S3, S2 and S3, which are described in detail below.

S2, receiving the signal output by the single liquid level sensor 250, and calculating the liquid level of the dye liquid in the liquid storage tank 151 according to the signal to obtain a detection value;

s3, comparing the detected value with the first set value and the third set value to determine whether the liquid level in the liquid reservoir 151 reaches the first set value, and determining whether the liquid level in the liquid reservoir 151 reaches the third set value.

Referring to fig. 18, fig. 18 is a flowchart of a dye liquor recycling method according to still another embodiment of the present application. The dye liquor recovery method also comprises S290, and the details of S290 are described as follows.

S290, a sixth determining step of determining whether the dye solution in the dyeing box 152 is a newly input dye solution or a dye solution with a preset number of times of recycling.

Executing the discharging step (S190) when the dye liquor in the dye liquor box is the dye liquor with the recovery times reaching the preset times; when the dye solution in the dyeing box 152 is a newly input dye solution, the dye solution recovery step is performed (S170). Please refer to the foregoing description for S190 and S170, which are not described herein again.

Referring to fig. 19, fig. 19 is a flowchart illustrating a dye liquor recycling method according to still another embodiment of the present application. The tip bias further includes a pressure control module 210, and the pressure control module 210 is configured to adjust the pressure of the reservoir 151. The pressure adjustment module refers to the related description above, and is not described herein again. Accordingly, the dye liquor recovery method comprises the steps of S4 and S4 which are described in detail as follows. In this embodiment, the dye liquor recovery method including S4 is incorporated into the embodiment described in fig. 8 and its related description. It will be appreciated that the dye liquor recovery process including S4 may also be incorporated into the dye liquor recovery process described in any of the previous embodiments.

S4, a pressure adjusting step of adjusting the pressure of the reservoir 151.

When the liquid storage tank 151 is at positive pressure, executing the dye liquid output step (S150); when the reservoir 151 is at a negative pressure, the dye liquor recovery step is performed (S170).

Please refer to the foregoing description for S150 and S170, which are not described herein again.

Although embodiments of the present application have been shown and described, it is understood that the above embodiments are illustrative and not restrictive, and that those skilled in the art may make changes, modifications, substitutions and alterations to the above embodiments without departing from the scope of the present application, and that such changes and modifications are also to be considered as within the scope of the present application.

Claims

1. a tablet pusher, is characterized in that, comprises:

Sampling mechanism, used to suck the sample to be tested;

Slide loading mechanism for loading slides;

The sample loading mechanism is used to load the sample to be tested on the glass slide;

a slide pushing mechanism, used for smoothing the sample to be tested on the glass slide to make a smear;

a dyeing solution mechanism, including a liquid storage tank and a dyeing box, the liquid storage tank is used for storing the dyeing solution, and the dyeing box is used for dyeing the sample to be tested on the smear;

Dye liquor output liquid path, one end of the dye liquor output liquid path is communicated with the liquid storage tank, and the other end is communicated with the dyeing box on the first dyeing station;

a dye liquor recovery liquid path, one end of the dye liquor recovery liquid path is communicated with the liquid storage tank, and the other end is communicated with the dyeing box on the second dyeing station;

a transport mechanism for transporting the dyeing box from the first dyeing station to the second dyeing station; and

a control mechanism, connected in communication with the liquid storage tank, the dyeing box and the conveying mechanism, the control mechanism is configured to: control the dye liquor in the liquid storage tank to the first dyer through the dye liquor output path The dyeing box on the position outputs the dye liquor, and the conveying mechanism is controlled to transport the dyeing box from the first dyeing station to the second dyeing station; control the dyeing box on the second dyeing station. The dye liquor after dyeing of the sample to be tested is recovered into the liquid storage tank through the dye liquor recovery liquid path.

2. The tablet pusher according to claim 1, wherein the control mechanism is further configured to: determine whether the number of times the dye liquor is recovered reaches a preset number, and when the number of times the dye liquor is recovered reaches a preset number When the number of times, the dyed dye liquor in the dyeing box on the second dyeing station is controlled to be discharged through the waste liquid channel.

3. The tablet pusher according to claim 2, wherein the control mechanism is further configured to: determine whether the amount of dye liquor in the liquid storage tank reaches a first set value, when the storage tank When the amount of dye liquor in the liquid pool reaches the first set value, the dye liquor in the liquid storage tank is controlled to be discharged through the waste liquid channel.

4 . The tablet pusher according to claim 3 , wherein the control mechanism is further configured to: determine whether the dye solution in the liquid storage tank is emptied, and when the dye liquid in the liquid storage tank is emptied. 5 . When the liquid is emptied, fresh dye liquid is refilled into the liquid reservoir.

5. The tablet pusher according to claim 4, wherein the control mechanism is further configured to: determine whether the amount of newly input dye liquor in the liquid storage tank reaches the second set value, and when the When the amount of the newly input dye liquor in the liquid storage tank reaches the second set value, the newly input dye liquor in the liquid storage tank is controlled to be output to all the dyeing stations on the first dyeing station through the dye liquor output liquid path. in the staining box.

6. The tablet pusher according to claim 4, wherein the control mechanism is further configured to: determine whether the amount of newly input dye liquor in the liquid storage tank reaches a third set value, and when the When the amount of newly input dye liquor in the liquid storage tank reaches the third set value, the input of new dye liquid into the liquid storage tank is stopped.

7. The tablet pusher according to claim 6, wherein the control mechanism is further configured to: determine whether the dye liquor in the dyeing box is a newly input dye liquor or the number of times of recycling reaches a preset number of times. Dye liquor; when the dye liquor in the dye liquor box is the dye liquor whose recovery times reach the preset times, the dye liquor in the dye liquor box on the second dyeing station is controlled to be discharged through the waste liquor channel; When the dye liquor in the dyeing box is the newly imported dye liquor, the newly input dye liquor after the dyeing of the sample to be tested in the dyeing box on the second dyeing station is controlled to be recovered to the described dye liquor through the dye liquor recovery liquid path. in the reservoir.

8. The film pusher according to claim 1, wherein the film pusher further comprises:

The pressure control module is used to adjust the pressure of the liquid storage tank. When the liquid storage tank is at a positive pressure, the liquid storage tank outputs dye liquor to the dyeing box through the dye liquor output liquid path. When the liquid storage tank is under negative pressure, the dye liquor after dyeing the sample to be tested is recovered to the liquid storage tank through the dye liquor recovery liquid path.

9. A dye liquor recovery method, applied to a slide pusher, wherein the slide pusher comprises a sample suction mechanism, a slide loading mechanism, a sample adding mechanism, a slide push mechanism, a dye liquor mechanism, and a transport mechanism, The dye liquor recovery method includes:

a sample suction step, wherein the sample suction mechanism sucks the sample to be tested;

a slide loading step, wherein the slide loading mechanism loads the slides;

In the sample adding step, the sample adding mechanism loads the sample to be tested on the glass slide;

In the step of pushing the slide, the slide pushing mechanism pushes the sample to be tested on the glass slide to make a smear;

The dyeing liquid mechanism includes a liquid storage tank and a dyeing box, the liquid storage tank is used for storing the dyeing liquid, and the dyeing liquid box is used for dyeing the sample to be tested on the smear;

In the dye liquor output step, the dye liquor in the liquid storage tank outputs the dye liquor to the dyeing box on the first dyeing station through the dye liquor output liquid path, and the dyeing box receives the dye liquor and dyes the sample to be tested;

a transporting step, the transport mechanism transports the dyeing box from the first dyeing station to the second dyeing station; and

In the dye liquor recovery step, the dye liquor after dyeing of the sample to be tested in the dyeing box on the second dyeing station is recycled into the liquid storage tank through the dye liquor recovery liquid path.

10. dye liquor recovery method as claimed in claim 9, is characterized in that, described dye liquor recovery method also comprises:

The first judgment step is to judge whether the number of times the dye liquor is recovered reaches a preset number of times;

In the discharging step, when the number of times the dye liquor is recovered reaches the preset number of times, the dye liquor after dyeing in the dyeing box on the second dyeing station is controlled to be discharged through the waste liquor channel.

11. dye liquor recovery method as claimed in claim 10, is characterized in that, said dye liquor recovery method also comprises:

The second judgment step is to judge whether the amount of dye liquor in the liquid storage tank reaches the first set value;

In the discharging step, when the amount of dye liquor in the liquid storage tank reaches a first set value, the dye liquor in the liquid storage tank is controlled to be discharged through the waste liquid channel.

12. dye liquor recovery method as claimed in claim 11 is characterized in that, after described discharging step, described dye liquor recovery method also comprises:

The third judgment step is to judge whether the dye liquor in the liquid storage tank is emptied;

In the priming step, when the liquid storage tank is emptied, new dye solution is reintroduced into the liquid storage tank.

13. dye liquor recovery method as claimed in claim 12, is characterized in that, after described perfusion step, described dye liquor recovery method also comprises:

The fourth judgment step is to judge whether the amount of newly input dye liquor in the liquid storage tank reaches the second set value;

In the dye liquor re-output step, when the amount of newly input dye liquor in the liquid storage tank reaches the second set value, the newly input dye liquor in the liquid storage tank is controlled to be output to the dye liquor output circuit through the dye liquor output circuit. In the dye liquor box on the first dyeing station.

14. dye liquor recovery method as claimed in claim 13, is characterized in that, described dye liquor recovery method also comprises:

The fifth judgment step is to judge whether the amount of newly input dye liquor in the liquid storage tank reaches the third set value;

The perfusion step is stopped, and when the amount of newly input dye solution in the liquid storage tank reaches a third set value, the input of new dye solution into the liquid storage tank is stopped.