CN114042545A - Centrifugation divides liquid subassembly and centrifuge - Google Patents
Centrifugation divides liquid subassembly and centrifuge Download PDFInfo
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- CN114042545A CN114042545A CN202111195191.8A CN202111195191A CN114042545A CN 114042545 A CN114042545 A CN 114042545A CN 202111195191 A CN202111195191 A CN 202111195191A CN 114042545 A CN114042545 A CN 114042545A
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- 238000000926 separation method Methods 0.000 claims abstract description 68
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B04—CENTRIFUGAL APPARATUS OR MACHINES FOR CARRYING-OUT PHYSICAL OR CHEMICAL PROCESSES
- B04B—CENTRIFUGES
- B04B5/00—Other centrifuges
- B04B5/04—Radial chamber apparatus for separating predominantly liquid mixtures, e.g. butyrometers
- B04B5/0407—Radial chamber apparatus for separating predominantly liquid mixtures, e.g. butyrometers for liquids contained in receptacles
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B04—CENTRIFUGAL APPARATUS OR MACHINES FOR CARRYING-OUT PHYSICAL OR CHEMICAL PROCESSES
- B04B—CENTRIFUGES
- B04B11/00—Feeding, charging, or discharging bowls
- B04B11/06—Arrangement of distributors or collectors in centrifuges
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B04—CENTRIFUGAL APPARATUS OR MACHINES FOR CARRYING-OUT PHYSICAL OR CHEMICAL PROCESSES
- B04B—CENTRIFUGES
- B04B13/00—Control arrangements specially designed for centrifuges; Programme control of centrifuges
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B04—CENTRIFUGAL APPARATUS OR MACHINES FOR CARRYING-OUT PHYSICAL OR CHEMICAL PROCESSES
- B04B—CENTRIFUGES
- B04B7/00—Elements of centrifuges
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B04—CENTRIFUGAL APPARATUS OR MACHINES FOR CARRYING-OUT PHYSICAL OR CHEMICAL PROCESSES
- B04B—CENTRIFUGES
- B04B9/00—Drives specially designed for centrifuges; Arrangement or disposition of transmission gearing; Suspending or balancing rotary bowls
- B04B9/02—Electric motor drives
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B04—CENTRIFUGAL APPARATUS OR MACHINES FOR CARRYING-OUT PHYSICAL OR CHEMICAL PROCESSES
- B04B—CENTRIFUGES
- B04B9/00—Drives specially designed for centrifuges; Arrangement or disposition of transmission gearing; Suspending or balancing rotary bowls
- B04B9/08—Arrangement or disposition of transmission gearing ; Couplings; Brakes
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- Centrifugal Separators (AREA)
Abstract
The application provides a centrifugation divides liquid subassembly and centrifuge, centrifugation divides liquid subassembly includes the driving piece, support piece, centrifugation container and monitoring piece, the driving piece is connected with support piece, centrifugation container sets up on support piece, wherein, centrifugation container includes the sample container, collect the container, pipeline and valve, the sample container passes through the pipe connection with the collection container, the valve setting is on this pipeline, monitoring piece includes camera and terminal, the camera is connected with the terminal electricity, the camera shoots towards the pipeline all around of valve, the terminal is used for receiving the image that the camera was shot. Can be at the centrifugal layering state of terminal real-time observation sample liquid in the sample container through the camera to utilize centrifugal force to collect the sample liquid after the layering, avoided artifical extraction in-process to take place the problem that the secondary mixes or pollute the sample, improved the precision, efficiency and the stability of centrifugal separation process.
Description
Technical Field
The application relates to centrifugation divides liquid technical field, concretely relates to centrifugation divides liquid subassembly and centrifuge.
Background
Centrifuge is a common minute liquid instrument, when separating sample liquid, and centrifuge produces centrifugal force with the help of the high-speed rotation of pivot, makes the liquid separation of different density, reaches the effect of preliminary minute liquid. Most of the prior similar technologies are used for manually extracting and separating layered liquid after centrifugal liquid separation of sample liquid, and the separation mode has low precision, low efficiency and poor stability, and is easy to cause secondary mixing; moreover, the current centrifuge usually cannot directly observe the separation condition of the sample liquid, and needs to take out the centrifuge tube filled with the sample liquid for observation, so that secondary mixing is easily caused in the taking and placing process, and the liquid separation effect is reduced.
Disclosure of Invention
The invention aims to provide a centrifugal liquid separating assembly and a centrifugal machine, and solves the problems that in the prior art, the centrifugal liquid separating process is low in precision, low in efficiency and poor in stability, and the liquid separating condition cannot be directly observed.
In order to realize the purpose of the invention, the invention provides the following technical scheme:
in a first aspect, the invention provides a centrifugal liquid-separating assembly, which comprises a driving part, a supporting part, a centrifugal container and a monitoring part, wherein the driving part is arranged on the driving part; the driving piece is connected with the supporting piece so as to drive the supporting piece to rotate; the centrifuge vessel is disposed on the support; wherein the centrifugation container comprises a sample container, a collection container, a pipeline and a valve, the sample container and the collection container are connected through the pipeline, and the valve is arranged on the pipeline; and the monitoring part comprises a camera and a terminal, the camera is electrically connected with the terminal, the camera faces the periphery of the valve, the pipeline shoots, and the terminal is used for receiving images shot by the camera.
In one embodiment, the valve is electrically connected to the terminal, and the terminal is used for controlling the opening and closing of the valve.
In one embodiment, the terminal recognizes an image taken by the camera and controls opening and closing of the valve according to the recognition result.
In one embodiment, the centrifuge container further comprises a heat seal located at the junction of the tube and the collection container, the heat seal being electrically connected to the terminal for controlling the heat seal to heat seal the collection container.
In one embodiment, the collecting container includes a transfer bag and a plug, the plug is disposed on the transfer bag, the transfer bag is connected to the pipe, and the heat sealing member heat-seals the transfer bag.
In one embodiment, the centrifuge container further comprises a sample base, the sample container, the collection container, the conduit, and the valve are disposed on the sample base, and the connection of the conduit to the sample container is proximate to the sample base.
In one embodiment, the centrifugation container comprises more than one collection container, the number of the conduits connecting the valve to the collection container is the same as the number of the collection containers, and the sample container is in communication with one of the collection containers when the valve is open.
In one embodiment, the driving member includes a centrifugal motor, the supporting member includes a coupling and a bracket, the centrifugal motor is connected to the coupling, and the bracket is disposed on a side of the coupling opposite to the centrifugal motor.
In one embodiment, the support further comprises a rotating shaft connected between the coupler and the bracket.
In a second aspect, the invention further provides a centrifuge, which comprises a shell and the centrifugal liquid separation assembly in any embodiment of the first aspect, wherein the centrifugal liquid separation assembly is arranged in the shell.
Through setting up driving piece and support piece, realize the rotary drive of driving piece to support piece, set up centrifugal container on support piece, realize the centrifugal separation of sample liquid among the centrifugal container, set up the observation that monitoring piece realized the sample liquid separation condition among the centrifugal container. The centrifugal container comprises a sample container, a collecting container, a pipeline and a valve, wherein the sample container is used for placing sample liquid, the collecting container is used for collecting partial sample liquid after centrifugal liquid separation, the pipeline is used for connecting the sample container and the collecting container, the valve is arranged on the pipeline and used for controlling the communication and the closing of the pipeline, the size of the pipeline can be adjusted through the valve, and the flow rate of the sample liquid flowing from the sample container to the collecting container is controlled; the monitoring part comprises a camera and a terminal, the camera is used for shooting the separation state of the sample liquid and transmitting the image to the terminal, and the separation state of the sample liquid can be directly observed on the terminal.
When centrifugal liquid separation is started, firstly, the valve is adjusted to close a pipeline between the sample container and the collection container, then, sample liquid is put into the sample container, and the driving piece drives the supporting piece to rotate, so that centrifugal layering of the sample liquid is realized; observe the real-time separation state of sample liquid on the terminal through the camera, after the affirmation separation finishes, the collecting vessel that the governing valve intercommunication corresponds, drive support piece is rotatory, makes partial sample liquid flow to collecting vessel under the effect of centrifugal force, and the affirmation is collected the back of finishing, closes the valve and stops rotatoryly, perhaps another collecting vessel of governing valve intercommunication realizes collecting in succession. The valve is regulated and controlled by arranging the pipeline and the valve between the sample container and the collecting container, so that the sample liquid is collected, and the problems of low precision, low efficiency and poor stability of manual liquid taking are solved; through setting up camera and terminal, realize the real time monitoring to sample liquid separation state, make whole separation process more directly perceived, avoided sample liquid to get the secondary of putting the in-process and mix.
Drawings
In order to more clearly illustrate the technical solutions of the embodiments of the present application, the drawings needed to be used in the embodiments will be briefly described below, and it is obvious that the drawings in the following description are only some embodiments of the present application, and it is obvious for those skilled in the art to obtain other drawings based on these drawings without creative efforts.
FIG. 1 is a schematic structural diagram of a centrifugal liquid separation assembly according to an embodiment;
FIG. 2 is a schematic diagram of the construction of a centrifuge vessel according to an embodiment;
FIG. 3 is a schematic structural view of a rotating shaft according to an embodiment;
FIG. 4 is a schematic diagram of a centrifuge according to an embodiment;
FIG. 5 is a system block diagram of a centrifuge of an embodiment.
Description of reference numerals:
100-centrifugal liquid separation component, 200-centrifuge;
10-driving piece, 11-centrifugal motor, 12-controller, 13-stepping motor, 14-motor screw rod;
20-a support, 21-a coupler, 22-a bracket, 23-a rotating shaft, 231-a first central upright post, 232-a second central upright post, 233-a bearing, 234-a transmission shaft, 235-a central sleeve, 236-a connecting sleeve, 237-a bearing baffle, 24-a hoop, 25-a sleeve and 26-a motor fixing ring;
30-centrifugal container, 31-sample container, 32-collection container, 321-transfer bag, 322-plug, 33-pipeline, 34-valve, 35-heat sealing piece, 36-sample base;
40-monitoring piece, 41-camera, 42-terminal;
50-shell, 501-cover, 502-opening, 503-fixed foot, 51-motor fixed plate, 52-spring.
Detailed Description
The technical solutions in the embodiments of the present application will be clearly and completely described below with reference to the drawings in the embodiments of the present application, and it is obvious that the described embodiments are only a part of the embodiments of the present application, and not all of the embodiments. The embodiments listed in the present application may be appropriately combined with each other.
Referring to fig. 1, a centrifugal separator assembly 100 according to an embodiment of the present disclosure is used for separating a mixture including a plurality of monomer liquids with different specific gravities, such as, but not limited to, blood, cell mixture, and a plurality of bacteria culture solutions. The centrifugal separating assembly 100 comprises a driving member 10, a supporting member 20, a centrifugal container 30 and a monitoring member 40.
Specifically, referring to fig. 1 and 2, the driving member 10 is connected to the supporting member 20 to drive the supporting member 20 to rotate, and the driving member 10 may be a motor, and the power supply mode includes, but is not limited to, commercial power supply or internal battery power supply. The centrifugation container 30 is provided on the support 20 to rotate together with the support 20.
The centrifuge container 30 comprises a sample container 31, a collection container 32, a pipeline 33 and a valve 34, wherein the sample container 31 and the collection container 32 are connected through the pipeline 33, and the valve 34 is arranged on the pipeline 33. The sample container 31 is used for placing the sample liquid, and the top end of the sample container 31 comprises a cover with a slightly larger diameter, so that the sample liquid can be conveniently taken and put, and the sealing of the sample container 31 is realized. The sample liquid in the sample container 31 is centrifugally layered along with the rotation of the centrifugal container 30, and before the sample liquid is added, a valve 34 of a pipeline 33 communicated between the sample container 31 and the collection container 32 is closed, so that the sample container 31 forms a closed cavity; the collection container 32 is used for collecting part of the centrifuged and layered sample liquid, and after the centrifugation and layering of the sample liquid are completed, the valve 34 communicating with the pipeline 33 between the sample container 31 and the collection container 32 is opened, and the support member 20 continues to rotate under the action of the driving member 10, so that the sample liquid in the sample container 31 enters the collection container 32 under the action of centrifugal force.
The monitoring member 40 includes a camera 41 and a terminal 42 (see fig. 5), the camera 41 is electrically connected to the terminal 42, the camera 41 takes a picture of the pipe 33 around the valve 34, the state of the sample liquid in the pipe 33 near the valve 34 can be observed through the camera 41, the camera 41 is fixed by, but not limited to, being connected to the support member 20, the terminal 42 is used for receiving the image taken by the camera 41, and the terminal 42 may include a display screen for displaying the image taken by the camera 41, so as to realize real-time monitoring of the state of the sample liquid and serve as a basis for adjusting the valve 34.
By providing the driving member 10 and the supporting member 20, a support is provided for the centrifugation container 30, the centrifugation container 30 is rotated, the centrifugation and stratification of the sample liquid in the centrifugation container 30 are realized, and a centrifugal force is provided for the separation and collection process of the sample liquid.
By providing the sample container 31 and the collection container 32, the centrifugal separation process of the sample liquid is divided into a centrifugal separation process and a separation and collection process, and in general, the volume of the sample container 31 is less than or equal to the total volume of the collection container 32, so that the sample liquid can be completely collected, the sample container 31 is connected with the collection container 32 through a pipeline 33 with a valve 34, the communication and the closing of the pipeline 33 can be controlled by adjusting the valve 34, the centrifugal liquid separation process and the separation and collection process are ensured to be smoothly carried out, and the two processes are carried out without interruption, the communication state of the pipeline 33 is changed by adjusting the valve 34, can directly realize the switching of the centrifugal liquid separation process and the separation and collection process, and simultaneously, the valve 34 can also adjust the opening and closing size of the pipeline 33, thereby controlling the flow rate of the sample liquid flowing from the sample container 31 to the collection container 32, and enabling the collection process of the layered sample liquid to be more stable and accurate.
Through setting up camera 41 and terminal 42, realize direct observation and real time monitoring to sample liquid separation state, make whole separation process more direct-viewing, be favorable to accurately judging the centrifugation degree of sample liquid and the collection rate among the separation collection process to in time adjust through terminal 42. Whole centrifugation divides the liquid process and need not get and put centrifugal container 30, can the direct observation centrifugation divide the liquid condition and realize the layering of sample liquid and collect, has practiced thrift artifical resource, has avoided the secondary of sample liquid to mix, makes whole centrifugation process accuracy better, efficiency is higher and stability increases.
In one embodiment, referring to fig. 2 and 5, the valve 34 is electrically connected to the terminal 42, the terminal 42 is used for controlling the valve position of the valve 34, and thus the connection or the disconnection of the pipe 33, and the valve position of the valve 34 can be changed by means including, but not limited to, manual, electric and pneumatic. The manual opening and closing is realized by changing the valve position of the valve body through manually rotating, pushing or pulling the sliding block; the electric opening and closing can generate electromagnetic attraction force through the spiral coil to drive the sliding block to change the valve position of the valve body; the pneumatic opening and closing can be realized by that a cylinder in the gas circuit pushes a piston to move so as to drive a slide block to change the valve position of the valve body.
Optionally, the valve position of the valve body is changed by a screw rod in an electric mode to realize opening and closing, please refer to fig. 1, the terminal 42 is electrically connected with the controller 12, the controller 12 is connected with the stepping motor 13 and used for driving the stepping motor 13 to rotate, the stepping motor 13 is connected with the motor screw rod 14 through a synchronous belt, the motor screw rod 14 is connected with the valve 34, and the motor screw rod 14 can drive the valve 34 to rotate through rotation of the motor screw rod 14, so as to change the valve position of the valve 34. Therefore, when adjusting the valve position of the valve 34, the terminal 42 first controls the controller 12 to drive the stepping motor 13 to rotate, the stepping motor 13 drives the motor screw 14 to rotate through the timing belt during the rotation, and the motor screw 14 drives the valve 34 to rotate during the rotation, thereby changing the valve position of the valve 34.
By electrically connecting the valve 34 with the terminal 42, the operation can be directly performed on the terminal 42, so that the change of the valve position of the valve 34 is realized, the problem that the change degree cannot be stably controlled when the valve position of the valve 34 is manually changed is solved, the adjusting process is more accurate, and the manual resources are saved; moreover, the state of the sample liquid in the pipeline 33 around the valve 34 can be directly observed on the terminal 42 through the camera 41, and the valve position of the valve 34 is changed according to the real-time observation result, so that the adjustment of the valve 34 is more targeted, the saving of manual resources is facilitated, and the accuracy and the efficiency of the centrifugal separation process are improved.
In one embodiment, referring to fig. 1 and 5, the terminal 42 recognizes the image captured by the camera 41 and controls the opening and closing of the valve 34 according to the recognition result. After receiving the image captured by the camera 41, the terminal 42 may manually analyze the image, and then adjust the valve position of the valve 34 according to the image, or may enable the terminal 42 to automatically determine the centrifugal liquid separation condition of the sample liquid according to the image, and adjust the valve 34 according to the image. If the terminal 42 judges that the centrifugal liquid separation of the sample liquid is not complete, the closing state of the valve 34 is kept; if the terminal 42 judges that the centrifugal separation of the sample liquid is finished, the automatic control valve 34 is communicated with the sample container 31 and the collection container 32 to realize the collection of the sample liquid.
The judgment mode of the terminal 42 for the centrifugal layering degree of the sample liquid includes, but is not limited to, color saturation of the image, whether the centrifugal layering degree reaches the standard or not is judged according to a difference value of the color saturation of the image, the sample liquid image after centrifugal liquid separation is finished can be stored locally at the terminal 42, the terminal 42 compares the local image with the real-time image of centrifugal liquid separation, and the centrifugal liquid separation is finished when the similarity of the two images reaches a certain value.
When the sample liquid contains more than two monomer liquids and more than two monomer liquids need to be collected, after the terminal 42 judges that the sample liquid is separated centrifugally, the valve 34 is controlled to be communicated with the sample container 31 and the first collecting container 32 to collect the first monomer liquid, after the terminal 42 judges that the first monomer liquid is collected, the valve position of the valve 34 is controlled again to close the connecting pipeline 33 between the sample container 31 and the first collecting container 32, and the sample container 31 is communicated with the second collecting container 32 until the second monomer liquid is collected, and so on, and finally all the monomer liquids in the sample liquid are collected. The terminal 42 determines the collection level during the collection of the single-fluid, including but not limited to color saturation and contrast similarity of the image, in a manner similar to the above-described determination of the centrifugal stratification level of the sample fluid.
The image shot by the camera 41 is automatically recognized through the terminal 42, an adjusting mode is added for changing the valve position of the valve 34, the terminal 42 intelligently analyzes and monitors the centrifugal liquid separation condition of the sample liquid in real time, the centrifugal liquid separation failure caused by negligence caused by artificial fatigue is avoided, and the manual workload is reduced; meanwhile, when analyzing and monitoring the centrifugal liquid separation condition of the sample liquid, the terminal 42 can also automatically adjust the valve 34 according to the analysis result, so that the full-automatic operation of the centrifugal liquid separation process and the separation and collection process of the sample liquid is realized, the requirement of artificial resources is further reduced, and the efficiency of the centrifugal separation process is improved.
In one embodiment, referring to fig. 2, the centrifuge container further comprises a heat seal 35, wherein the heat seal 35 is located at the connection between the tube 33 and the collection container 32, and is used for heat sealing the collection container 32 by heating without heating the tube 33. The heat seal 35 is electrically connected to the terminal 42, and when the terminal 42 determines that the sample liquid has been collected in the collection container 32, the heat seal 35 is controlled to heat seal the collection container 32, or the timing at which the heat seal is necessary is manually determined, and the heat seal 35 is operated to heat seal the collection container 42. The heat-sealed collection container 32 can be taken out directly from the pipeline 33, or after the heat-sealed part of the collection container 32 is cut, the collection of the layered sample liquid is completed.
In one embodiment, referring to fig. 2, the collection container 32 includes a transfer bag 321 and a plug 322, the plug 322 is disposed on the transfer bag 321, the transfer bag 321 is connected to the pipe 33, and the plug 322 is disconnected from the pipe 33. The transit bag 321 is used for collecting the sample liquid, and the material of the transit bag 321 includes, but is not limited to, transparent plastic, and the usage mode includes, but is not limited to, disposable usage. In the process of collecting the sample liquid by the transfer bag 321, the plug 322 is closed to ensure that the transfer bag 321 is only communicated with the pipeline 33, and after the sample liquid is collected by the transfer bag 321, the heat sealing member 35 performs heat sealing on the joint of the transfer bag 321 and the pipeline 33, so that the whole collection container 32 is closed, and the sample liquid can be conveniently taken out from the centrifugal container 30. When the sample liquid in the transit bag 321 needs to be extracted, the plug 322 can be opened to extract the sample liquid from the plug 322.
Through setting up end cap 322, make transfer bag 321 increase the sealing that is convenient for to open and shut, after transfer bag 321 by the heat-seal, can realize the extraction to the sample liquid through this end cap 322, avoided transfer bag 321 by the heat-seal after, draw sample liquid and need cut a breach to transfer bag 321, increase the contaminated possibility of sample liquid, also be favorable to the sample liquid once only can't use storage when finishing, make things convenient for next time to continue to draw.
In one embodiment, referring to fig. 2, the centrifuge container 30 further comprises a sample base 36, wherein the sample container 31, the collection container 32, the conduit 33 and the valve 34 are disposed on the sample base 36, and the connection between the conduit 33 and the sample container 31 is close to the sample base 36. The fixing part corresponding to the sample base 36 is arranged on the bracket 22, so that the sample base 36 can be fixed on the bracket 22, and further the centrifugal container 30 can be fixed on the bracket 22. The fixing component includes but is not limited to the clamp 24, referring to fig. 1, when the clamp 24 is used as the fixing component, the clamp 24 can be tightened or loosened to directly fix or disassemble the centrifuge container 30, so as to facilitate the taking and placing of the centrifuge container 30, the tightening and loosening of the clamp 24 can be adjusted by bolts, and more than one clamp 24 can be used for fixing a single centrifuge container 30.
When centrifugal liquid separation is performed on a sample liquid containing a plurality of monomer liquids, the sample liquid is placed in the sample container 31, the sample liquid is subjected to centrifugal force and gravity under the rotation of the sample container 31 at a certain speed, the monomer liquids in the sample liquid are layered according to the specific gravity after a period of time, the monomer liquid with the highest specific gravity is distributed on the side, away from the rotation center, of the sample container 31, the monomer liquid with the lower specific gravity is distributed on the side, close to the rotation center, of the sample container 31, and the smaller specific gravity is closer to the rotation center, so that the sample liquid is layered in the sample container 31. Wherein, the sample liquid is acted by two forces all the time in the process of centrifugal stratification, namely the centrifugal force far away from the rotation center and the gravity pointing to the ground, so the stratified state of the sample liquid is wholly deviated, the joint of the pipeline 33 and the sample container 31 is close to the sample base 36, and the collection of the sample liquid is more facilitated.
Optionally, the connection portion of the sample container 31 and the conduit 33 is in funnel-shaped connection, and the area of the connection portion is gradually reduced from the sample container 31 to the conduit 33, such a shape is beneficial to the sample liquid in the sample container 31 flowing into the conduit 33, and the sample liquid is convenient to collect.
The sample base 36 is arranged, so that the sample container 31, the collection container 32, the pipeline 33 and the valve 34 are fixed, meanwhile, the corresponding fixing parts are arranged on the bracket 22 corresponding to the sample base 36, so that the whole centrifugal container 30 can be fixed and disassembled, the centrifugal separation process of the sample liquid is more stable, the centrifugal container 30 is easier to disassemble, and the sampling of the sample liquid or the replacement of different centrifugal containers 30 are facilitated; set up the junction of pipeline 33 and sample container 31 in the position that is close to sample base 36, considered sample liquid and received centrifugal force and gravity's combined action simultaneously at centrifugal layering in-process, made the design more scientific and reasonable, also be favorable to the collection of each monomer liquid in the sample liquid, improved the accuracy and the stability of sample liquid centrifugation minute liquid in-process.
In one embodiment, referring to FIG. 2, centrifuge vessel 30 includes more than one collection vessel 32, and the same number of tubes 33 and collection vessels 32 connect valve 34 to collection vessels 32, with sample vessel 31 communicating with one of collection vessels 32 when valve 34 is open.
When the centrifugal container 30 comprises a sample container 31 and corresponds to a collection container 32, the sample container 31 is connected with the collection container 32 through a pipeline 33, a two-way valve is arranged on the pipeline 33, and the communication and the closing of the pipeline 33 can be realized by adjusting the two-way valve; when the centrifugation container comprises one sample container 31 and corresponds to three collection containers 32, a branch pipeline is arranged on a pipeline 33 between the sample container 31 and the collection containers 32, wherein one pipeline 33 is connected between the sample container 31 and a valve 34, the pipeline 33 is branched into three branch pipelines at the valve 34, the three branch pipelines are respectively connected with the three collection containers 32, the valve 34 is a four-way valve, the closing of the pipeline 33 or the communication between the sample container 31 and one of the collection containers 32 can be realized by adjusting the four-way valve, and when the number of the collection containers 32 is changed, the structure of the pipeline 33 connecting the sample container 31 and the collection containers 32 is similar. When the sample fluid contains only two monomer fluids but the centrifuge container 30 includes more than two collection containers 32, the sample fluid can be collected using only two of the collection containers 32, as regulated by the valve 34.
Through setting up a plurality of collection container 32, can set up the collection container 32 that corresponds quantity to different sample liquid, satisfy different centrifugation liquid demands. When the sample liquid contains various monomer liquids, the manual extraction of each monomer liquid after centrifugal layering is very difficult and complicated, in the extraction process, the monomer liquids in layered states are very easy to generate secondary mixing in the sampling process, so that the separation purity is influenced, and in the manual extraction and separation process, workers also need to replace a plurality of collecting containers 32, so that the stability of the collection process is further reduced; through setting up a plurality of collection containers 32 to cooperate corresponding valve 34, can collect in succession the sample liquid that contains multiple monomer liquid, and the layering state between each monomer liquid is more stable in the collection process, has still avoided the possibility of external environmental pollution separation sample in the separation process, is favorable to improving the centrifugation of sample liquid and divides the precision and the stability of liquid process.
In one embodiment, referring to fig. 1, the driving member 10 includes a centrifugal motor 11, the supporting member 20 includes a coupling 21 and a bracket 22, the centrifugal motor 11 is connected to the coupling 21, the centrifugal motor 11 drives the coupling 21 to rotate, the bracket 22 is disposed on a side of the coupling 21 opposite to the centrifugal motor 11, the bracket 22 is driven by the coupling 21 to rotate, and the centrifugal container 30 is disposed on the bracket 22 and rotates together with the bracket 22. When the number of the branches of the support 22 is two or more, the branches are generally symmetrical about a central axis of the coupling 21, so as to maintain the balance of the support 22 during the centrifugal rotation process; the connection between the bracket 22 and the coupling 21 can be, but is not limited to, a detachable connection, which provides more design possibilities for the connection between the bracket 22 and the coupling 21, and can also improve the support performance by adding some parts.
Referring to fig. 1, the design between the bracket 22 and the coupler 21 includes, but is not limited to, that a sleeve 25 may be disposed on the outer periphery of the coupler 21 to protect the coupler 21 and play a certain supporting role, so as to reduce the torque borne by the coupler 21 and increase the service life of the coupler 21; a motor fixing ring 26 may be disposed between the centrifugal motor 11 and the coupling 21 for fixing the centrifugal motor 11, increasing the stability of the coupling 21 in the rotation process, and reducing the wear of the centrifugal motor 11 and the coupling 21 during operation.
By arranging the centrifugal motor 11 and the coupling 21, the centrifugal motor 11 drives the coupling 21 to rotate, and further drives the bracket 22 connected with the coupling 21 to rotate, so that the torque borne by the coupling 21 is reduced, and the design of the combination between the coupling 21 and the bracket 22 provides more possibilities for other improved designs of the coupling 21 and the bracket 22 and also provides more possibilities for the design of the support member 20, thereby being beneficial to realizing the application of various centrifugal liquid separation occasions; the number and the shape of the branches of the bracket 22 can be regulated, and the branches of the bracket 22 with proper number and shape can be selected according to different centrifugal liquid separation requirements, so that the centrifugal liquid separation structure is suitable for the centrifugal requirements of various sample liquids.
In one embodiment, referring to fig. 1, the supporting member 20 further includes a rotating shaft 23, and the rotating shaft 23 is connected between the coupling 21 and the bracket 22. The rotating shaft 23 includes but is not limited to the structure shown in fig. 3, in the structure of the rotating shaft 23 shown in fig. 3, the rotating shaft 23 includes a first center pillar 231 and a second center pillar 232, wherein the first center pillar 231 is connected with the transmission shaft 234 through a connection kit 236, the first center pillar 231 and the connection kit 236 are disposed on the second center pillar 232, the bearing 233, the transmission shaft 234 and the center bushing 235 are disposed in the second center pillar 232, the bearing 233 is connected with the transmission shaft 234, during the rotation of the transmission shaft 234, the bearing 233 converts the sliding friction generated by the rotation of the transmission shaft 234 into the rolling friction, which is beneficial to increasing the service life of the rotating shaft 23 and making the rotation process of the transmission shaft 234 more stable, the center bushing 235 is sleeved on the periphery of the bearing 233 to support and protect the bearing 233, the bearing baffle 237 is disposed on the opposite side of the connection of the second center pillar 232 and the first center pillar 231, the bearing 233, the transmission shaft 234 and the center sleeve 235 are fixed, and the rotating shaft 23 and other parts of the support 20 are fixed to each other, when the rotating shaft 23 is connected between the coupler 21 and the bracket 22, the rotating shaft 23 is connected with the coupler 21 through the second center pillar 232, and the rotating shaft 23 is connected with the bracket 22 through the first center pillar 231.
The rotating shaft 23 is arranged and used for bearing bending moment and torque caused by movement in the rotating process, so that the bending moment and the torque borne by the coupler 21 are reduced, when the support member 20 is worn in a certain working time and the movement process of the support 22 is not stable enough, the support member 20 can normally work by directly replacing the rotating shaft 23 under a common condition, and the working stability of the support member 20 is improved; the rotating shaft 23 also converts sliding friction between the rotating shaft part comprising the coupler 21 and the rotating shaft 23 and the support 22 into rolling friction, so that the service life of the coupler 21 and the service life of the support 22 are prolonged, the stability of the support 22 in the rotating process is improved, and the efficiency and the stability of the centrifugal liquid separation process of the sample liquid are further improved.
Referring to fig. 4, the centrifuge 200 includes a housing 50 and the centrifugation liquid separation assembly 100 in any of the foregoing embodiments, and the centrifugation liquid separation assembly 100 is disposed in the housing 50. The casing 50 may be in the shape of a cube, and the top end of the casing 50 includes a cover 501 for protecting the components inside the casing 50, and the casing 50 further includes an opening 502, the opening 502 may be closed by the cover 501, the opening 502 communicates with the inner cavity of the casing 50, and the centrifugal separation assembly 100 may be operated or the sample liquid may be taken and placed through the opening 502. The bottom of the housing 50 is further provided with a fixing leg 503 to make the centrifuge 200 more stable when placed on a plane.
The centrifuge 200 may further include other components, including but not limited to a motor fixing plate 51 and a spring 52, the motor fixing plate 51 and the spring 52 are both disposed in the casing 50, the centrifugation liquid separation assembly 100 is disposed on the motor fixing plate 51, the motor fixing plate 51 is connected to the casing 50 through a fixing column located at the center of the motor fixing plate 51, and the spring 52 is disposed around the fixing column, and the spring 52 connects the motor fixing plate 51 to the casing 50.
Through setting up casing 50 for hold centrifugation and divide liquid subassembly 100, motor fixed plate 51 is used for fixed centrifugation to divide liquid subassembly 100, sets up spring 52 between motor fixed plate 51 and casing 50 and connects, can reduce the vibration that centrifugation divides liquid subassembly 100 to produce at the rotation in-process, makes whole centrifuge 200 more stable in the course of the work, is favorable to improving the stability of centrifugation liquid process.
The foregoing is a partial description of the present application, and it should be noted that, for those skilled in the art, various modifications and decorations can be made without departing from the principle of the present application, and these modifications and decorations are also regarded as the protection scope of the present application.
Claims (10)
1. A centrifugal liquid separation assembly is characterized by comprising a driving piece, a supporting piece, a centrifugal container and a monitoring piece;
the driving piece is connected with the supporting piece so as to drive the supporting piece to rotate;
the centrifuge vessel is disposed on the support;
wherein the centrifugation container comprises a sample container, a collection container, a pipeline and a valve, the sample container and the collection container are connected through the pipeline, and the valve is arranged on the pipeline; and
the monitoring part comprises a camera and a terminal, the camera is electrically connected with the terminal, the camera faces the periphery of the valve, the pipeline shoots, and the terminal is used for receiving images shot by the camera.
2. The centrifugal liquid separation assembly of claim 1, wherein the valve is electrically connected to the terminal, and the terminal is used for controlling the opening and closing of the valve.
3. The centrifugal liquid separation assembly according to claim 2, wherein the terminal recognizes the image shot by the camera and controls the valve to open or close according to the recognition result.
4. A centrifugal liquid separation assembly according to claim 1 wherein said centrifuge container further comprises a heat seal at the junction of said tubing and said collection container, said heat seal being electrically connected to said terminal for controlling said heat seal to heat seal said collection container.
5. The centrifugal liquid separation assembly of claim 4, wherein the collection container comprises a transfer bag and a plug, the plug is arranged on the transfer bag, the transfer bag is connected with the pipeline, and the heat sealing piece is used for performing heat sealing on the transfer bag.
6. The centrifugal liquid separation assembly of claim 1 wherein the centrifugal container further comprises a sample base, the sample container, the collection container, the conduit and the valve being disposed on the sample base, the connection of the conduit to the sample container being proximate to the sample base.
7. A centrifugal liquid separation assembly according to claim 1 wherein said centrifuge container comprises more than one of said collection containers, said conduits connecting said valve to said collection containers are the same number as said collection containers, and said sample container is in communication with one of said collection containers when said valve is open.
8. A centrifugal liquid separating assembly according to claim 1 wherein said driving member comprises a centrifugal motor, and said supporting member comprises a coupling and a bracket, said centrifugal motor being connected to said coupling, said bracket being disposed on a side of said coupling opposite to said centrifugal motor.
9. The centrifugal liquid separation assembly of claim 8 wherein the support member further comprises a shaft coupled between the coupling and the frame.
10. A centrifuge, comprising a housing and a centrifugation and separation assembly as recited in any one of claims 1 to 9, said centrifugation and separation assembly being disposed within said housing.
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| CN202111195191.8A CN114042545A (en) | 2021-10-13 | 2021-10-13 | Centrifugation divides liquid subassembly and centrifuge |
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| CN202111195191.8A CN114042545A (en) | 2021-10-13 | 2021-10-13 | Centrifugation divides liquid subassembly and centrifuge |
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