CN114009584A - Preparation method of additive for promoting growth and development of intestinal structures of young animals - Google Patents

Abstract

The invention discloses a preparation method of an additive for promoting the growth and development of intestinal structures of young animals, which comprises the following steps: screening and culturing specific probiotic strains with the metabolic cyclic dipeptide functional peptide: lactobacillus plantarum L-2003, Lactobacillus sporogenes C-2003; step two: liquid state fermentation is carried out to obtain a liquid mixture of the specific probiotics and the metabolite cyclic dipeptide functional peptide, and liquid synbiotics of the probiotics and the metabolite are prepared; step three: solid-state treatment, separation and purification to obtain a solid mixture of the specific probiotics and the metabolite cyclic dipeptide functional peptide, and preparing powder synbiotics of the probiotics and the metabolite; step four: screening prebiotics which are beneficial to the growth and the breeding of the specific probiotic strains and have good stability; step five: adding prebiotics into liquid synbiotics or powder synbiotics, and compounding to obtain the additive. The invention has the following advantages: the finished product is rich in specific prebiotics, specific probiotics and specific prebiotics, and has a mixed type probiotic with intestine strengthening function and outstanding effect.

Description

Preparation method of additive for promoting growth and development of intestinal structures of young animals

Technical Field

The invention relates to the technical field of intestinal tract structure growth and development of young animals, in particular to a preparation method of an additive for promoting the intestinal tract structure growth and development of the young animals.

Background

In the animal intestinal probiotics family, the probiotics which play a positive role in the intestinal tract not only comprise the probiotics, but also comprise food-prebiotics of the probiotics and metabolites of the probiotics, some probiotics have a synergistic effect with other probiotics, the products of some probiotics are food of other probiotics, and the mutual synergy exists among the prebiotics and the probiotics, so that the synergy among the probiotics is researched, the intestinal probiotics can be strengthened to play a role in strengthening the intestinal tract, and the effect of 1+1 is more than 2.

From the food of the probiotics, namely prebiotics, to the probiotics and then to the metabolite of the probiotics, namely prebiotics, the more definite and powerful the functionality, for example, enzyme preparations, antimicrobial peptides, cyclic dipeptides, vitamins, trace elements and the like which are the metabolites of the probiotics have definite functionality.

Based on a certain probiotic with definite functions, the core of the invention technology is to find out the probiotics capable of producing the metabolite, culture, breed and expand the flora to obtain the metabolite, find out the prebiotics capable of promoting the reproduction and metabolism of the flora and play the synergistic effect of a specific probiotic system.

Disclosure of Invention

In order to solve the technical problems, the technical scheme provided by the invention is as follows: a preparation method of an additive for promoting the growth and development of intestinal structures of young animals comprises the following steps:

the method comprises the following steps: screening and culturing specific probiotic strains with the metabolic cyclic dipeptide functional peptide: lactobacillus plantarum L-2003, Lactobacillus sporogenes C-2003;

step two: liquid state fermentation is carried out to obtain a liquid mixture of the specific probiotics and the metabolite cyclic dipeptide functional peptide, and liquid synbiotics of the probiotics and the metabolite are prepared;

step three: solid-state treatment, separation and purification to obtain a solid mixture of the specific probiotics and the metabolite cyclic dipeptide functional peptide, and preparing powder synbiotics of the probiotics and the metabolite;

step four: screening prebiotics which are beneficial to the growth and the breeding of the specific probiotic strains and have good stability;

step five: adding prebiotics into liquid synbiotics or powder synbiotics, and compounding to obtain the additive.

After the steps are adopted, the invention has the following advantages: the invention prepares a mixed type probiotic rich in specific prebiotics, specific probiotics and specific probiotics through the processes of screening, culturing, fermenting, solidifying, purifying, mixing and the like, so that the mixed type probiotic has a clear intestine-strengthening function and a remarkable effect, aims to strengthen the clear functionality of the probiotics, enables the intestine-strengthening function of the intestinal probiotics to have pertinence and actual effect, and has visualization without complex analysis of results.

Drawings

FIG. 1 is a comparison under microscope between the intestinal tract of chickens bred with the additive and the intestinal tract of normally bred chickens not using the additive, which are prepared by the method for promoting the growth and development of intestinal structures of young animals.

FIG. 2 is a schematic diagram of abnormal egg rate in the fourth example of the preparation method of the additive for promoting intestinal structure growth and development of young animals.

Fig. 3 is a graph comparing eggs laid in the fourth example of the preparation method of the additive for promoting intestinal structure growth and development of young animals.

FIG. 4 is a diagram showing the Salmonella positive rate and the survival rate of chicks after the purification of parental generation in example four of the method for preparing the additive for promoting the growth and development of intestinal structures of young animals according to the present invention.

FIG. 5 is a schematic diagram of the molecular formula and molecular weight of seventeen cyclic dipeptides of the preparation method of the additive for promoting the intestinal structure growth and development of young animals.

Detailed Description

A preparation method of an additive for promoting the growth and development of intestinal structures of young animals comprises the following steps:

the method comprises the following steps: screening and culturing specific probiotic strains with functional peptides such as metabolic cyclic dipeptide: lactobacillus plantarum L-2003, Lactobacillus sporogenes C-2003;

step two: liquid state fermentation is carried out to obtain a liquid mixture of the specific probiotics and the functional peptides such as the metabolite cyclic dipeptide and the like, and liquid synbiotics of the probiotics and the metabolite are prepared;

step three: solid-state processing, separating, and purifying to obtain solid mixture of the above specific probiotics and metabolite cyclic dipeptide functional peptides, and making into synbiotics powder of probiotics and metabolite;

step four: and (2) screening the prebiotics which are favorable for the growth and the breeding of the specific probiotic strains and have good stability: polysaccharides, oligosaccharides, microalgae, flavonoids, and the like;

step five: adding prebiotics into liquid synbiotics or powder synbiotics, and compounding to obtain the additive.

The preparation process comprises the following steps:

(1) and strain: lactobacillus plantarum: lactic acid strain L-2003: 10¹⁰cfu/g。

(2) Preparing liquid: culturing and fermenting the culture solution.

(3) And liquid fermentation conditions: the culture solution is inactivated and fermented for 72 hours at the pH value of 4 and the temperature of 30 ℃.

(4) And preparing powder: and (5) drying and spraying in vacuum.

In conclusion, the mixed type probiotics rich in specific prebiotics, specific probiotics and specific probiotics is prepared by the processes of screening, culturing, fermenting, solidifying, purifying, mixing and the like, so that the mixed type probiotics have clear intestine-strengthening function and outstanding effect.

In order to make the objects, technical solutions and advantages of the embodiments of the present invention more apparent, further experiments and data analysis will be performed on the additives produced by the present invention in combination with the embodiments of the present invention.

In the specific implementation of the invention, experiments are carried out in different chicken farms, and the following data are obtained through the experiments:

example one: the experimental site: six towns and large cai in plain county, tezhou; the experimental variety is as follows: roman 817; the number of the experimental sheds is as follows: 37000 the breeding is generally carried out for 55 days, 3.5 jin is taken out of the column, and the feed-meat ratio is 1.6-1.7; the additive products used in the present invention total: 43L.

Experimental data:

experimental day 8 weighing data:

experimental day 15 weighing data:

intestinal length measurement on experimental day 15:

the experimental conditions are as follows:

the analysis shows that 1, each chicken experimental group has more profit than the control group by 1.7 yuan, which is 1.2 times of the control group; 2. the feed-meat ratio is obviously reduced by nearly 2 percent; 3. weight average height analysis shows: (1) the feed-meat ratio of each chicken experimental group is reduced by nearly 2 percent (2), the average weight is 13.8 percent, and the growth speed is obviously accelerated; 4. the death and culling rate of the experimental group is 20 percent, and the reason why the death and culling rate of the experimental group is high in the early stage is that: the experimental group of the barns is earlier than the experimental group of the barns in construction, the early-stage constructed barns have bursal disease virus, and each group of the barns is required to have the bursal disease, so that the early-stage casualties of the experimental group are severe.

Example two: the experimental site: the Tongdai chicken farm in the development area of Binzhou city; the experimental variety is as follows: white feather broiler chicken; the number of the experimental sheds is as follows: 50000 pieces; 5 bottles of water are taken every day, and the water is concentrated and continuously used for 14 days; additive products of the invention were used together in total: 70L

Experimental data:

experimental day 8 weighing data:

experimental day 15 weighing data:

intestinal length measurement on experimental day 15:

intestinal length measurement on experimental day 15 (one longest and one shortest data mean, respectively):

example three: a chicken house 25500 feather and green-foot pheasant chick is used as an experimental group, 2L of the additive is added into each ton of water, and the chick is drunk for 14 days from 1 day old. The other 5 henhouses were fed with 13 million feathers as a control group as normal.

Data acquisition:

1. randomly selecting 20 live chickens from a control group and an experimental group on the 7 th day, the 14 th day and the experimental group respectively, weighing, repeating for 5 times (equivalent to the sample number of 100 chickens), and averaging the obtained weights to obtain the weight data of the group; meanwhile, the uniformity and the excrement condition of the chickens are observed and recorded.

2. On the 14 th day of the experiment, 4 live chickens are randomly selected from a control group and an experimental group respectively for dissection, the intestinal lengths of the live chickens are measured, and the average of the obtained data is used as the intestinal length data of the group; and observing the conditions of intestinal mucosa and the like.

3. The digital recording mode is adopted.

The experimental results are as follows:

1. weighing: the weight of the experimental group bodies on the 7 th day and the 14 th day is larger than that of the control group, and the weight is increased by nearly 30 percent.

2. And (4) intestinal tract dissection, wherein the intestinal tract length of the experimental group is obviously larger than that of the control group, and the intestinal mucosa is intact.

Group of	Average length of small intestine	Morphology of small intestine
			Control group	91.25cm	Thin intestinal wall and more feed particles.
Experimental group	107.75cm	Thick, thick intestine, intact mucous membrane, mostly minced.
			Increase in growth	16.5cm
Rate of increase	18.08％

Example four: breeding hens

The number of test chickens: 6580 hen houses No. 2 and 6050 hen houses No. 7 of parent white feather broiler breeders; week age of start test: 38 weeks; before the test: the positive rate of salmonella identified by parental breeding hen serotypes is 100%, and the death rate of offspring chicks is about 25%; and (3) test period: 2018.8.31-2018.9.9 (total 10 days); the test method comprises the following steps: adding the additive (powder) of the invention; and (3) test results: the purifying condition of the salmonella is analyzed by carrying out statistical analysis on abnormal eggs (malformed eggs, double-yellow eggs, broken eggs, preserved eggs and the like) of the laying hens.

The experimental results are as follows: no. 2 shed abnormal egg rate: 2.3% -4%; no. 7 shed abnormal egg rate: 2.6% -4%; after the parent-generation chick salmonella is purified, the positive rate of the salmonella is controlled within 10 percent, and the survival rate of the chick is improved by 15 percent.

Claims (1)

1. A preparation method of an additive for promoting the growth and development of intestinal structures of young animals is characterized by comprising the following steps: the method comprises the following steps:

the method comprises the following steps: screening and culturing specific probiotic strains with the metabolic cyclic dipeptide functional peptide: lactobacillus plantarum L-2003, Lactobacillus sporogenes C-2003;

step two: liquid state fermentation is carried out to obtain a liquid mixture of the specific probiotics and the metabolite cyclic dipeptide functional peptide, and liquid synbiotics of the probiotics and the metabolite are prepared;

step three: solid-state treatment, separation and purification to obtain a solid mixture of the specific probiotics and the metabolite cyclic dipeptide functional peptide, and preparing powder synbiotics of the probiotics and the metabolite;

step four: screening prebiotics which are beneficial to the growth and the breeding of the specific probiotic strains and have good stability;

step five: adding prebiotics into liquid synbiotics or powder synbiotics, and compounding to obtain the additive.

Images