CN114008460B

CN114008460B - A sample analysis device and a sample analysis method

Info

Publication number: CN114008460B
Application number: CN201980097765.2A
Authority: CN
Inventors: 李鑫; 闫华文; 吕富尧; 柴亮; 李爱博
Original assignee: Shenzhen Mindray Bio Medical Electronics Co Ltd; Beijing Shen Mindray Medical Electronics Technology Research Institute Co Ltd
Current assignee: Shenzhen Mindray Bio Medical Electronics Co Ltd; Beijing Shen Mindray Medical Electronics Technology Research Institute Co Ltd
Priority date: 2019-12-31
Filing date: 2019-12-31
Publication date: 2025-03-04
Anticipated expiration: 2039-12-31
Also published as: WO2021134610A1; CN114008460A

Abstract

A sample analysis device and a sample analysis method have one or more reagent dispensing parts (60), the number of the reagent dispensing parts (60) is equal to the number of processing units (50), each reagent dispensing part (60) comprises a plurality of reagent needles (61), a guide assembly (62) for guiding the plurality of reagent needles (61) to move linearly, and a second driving assembly (63) for driving the plurality of reagent needles (61) to move linearly along the guide assembly (62), and the guide assembly (62) is arranged along a direction determined by indexing in reagent sucking positions and reagent adding of the processing units (50) of the corresponding reagent dispensing part (60) so that the reagent needles (61) suck reagents from the reagent sucking positions and discharge the reagents into reaction cups indexed in the reagent adding of the processing units (50) corresponding to the reagent dispensing parts (60).

Description

Sample analysis device and sample analysis method

Technical Field

The present invention relates to a sample analysis device and a sample analysis method.

Background

Sample analyzers such as biochemical analyzers, immunoassay analyzers, coagulation analyzers, and cell analyzers are devices for analyzing and measuring samples, and generally measure the characteristics, chemical components, concentration, and the like of a sample itself by adding a reagent to the sample and then measuring the sample after reaction with the reagent in a predetermined manner.

The improvement of the test speed of the sample analyzer is a target sought by the technician, the miniaturization of the sample analyzer is one of the targets sought by the technician, and in order to improve the test speed of the sample analyzer, one idea is to add related components, such as a plurality of sample application needles, but the increase of the related components brings about an increase in the volume of the instrument, contrary to the miniaturization of the instrument sought by the technician. Therefore, how to solve or balance the contradiction between the speed increase and the miniaturization of the sample analysis device is an object of the present invention.

Disclosure of Invention

The present invention generally provides a sample analyzer and a sample analysis method, and is described in detail below.

According to a first aspect, a sample analysis device comprises:

A housing;

A cuvette loading section for supplying and carrying an empty cuvette;

the sample feeding part is used for dispatching a sample rack carrying samples to a sample suction position;

a sample dispensing part for sucking a sample from a sample sucking position and discharging the sample into a reaction cup located at a sample adding position;

The reagent carrying part is arranged in a disc-shaped structure and is provided with a plurality of positions for carrying a first reagent container of a first reagent and a plurality of positions for carrying a second reagent container of a second reagent, and comprises a first driving component for driving the reagent carrying part to rotate, wherein the first driving component drives the reagent carrying part to rotate and drives the first reagent container to rotate so as to rotate the first reagent container to a first reagent sucking position;

A reaction component for carrying a cuvette and incubating a sample in the cuvette, the reaction component being configured with at least one incubation indexing for placement of the cuvette;

A measuring means for carrying the cuvette and detecting a sample in the cuvette, the measuring means being provided with at least one index for measuring a position where the cuvette is placed;

The first reagent dispensing component comprises a first cross beam and a first group of reagent needles, wherein the first group of reagent needles at least comprises a plurality of first reagent needles, and the plurality of first reagent needles are arranged on the first cross beam and do linear motion along the long axis direction of the first cross beam so as to suck the first reagent from the first reagent sucking position and discharge the first reagent into a reaction cup positioned in the inversion of incubation;

the second reagent dispensing component comprises a second cross beam and a second group of reagent needles, wherein the second group of reagent needles at least comprises a plurality of second reagent needles, and the plurality of second reagent needles are arranged on the second cross beam and do linear motion along the long axis direction of the second cross beam so as to suck a second reagent from a second reagent sucking position and discharge the second reagent into a reaction cup positioned at the position where the reagent is positioned in the measuring process;

The dispatching component is used for dispatching the reaction cup, and the dispatching component dispatches the reaction cup with the first reagent added in the inversion of incubation to the reaction component and dispatches the reaction cup with the second reagent added in the inversion of measurement to the measurement component.

According to a second aspect, there is provided in one embodiment a sample analysis device comprising:

A cuvette loading section for supplying and carrying an empty cuvette;

A reagent carrying member which carries a reagent container and is rotatable to rotate the reagent container to a reagent sucking position;

The processing units are used for receiving the reaction cup bearing the sample and processing the sample of the reaction cup, wherein each processing unit is provided with a corresponding reagent adding index;

One or more reagent dispensing parts, the number of the reagent dispensing parts being equal to the number of the processing units, and one of the reagent dispensing parts corresponding to one of the processing units, each reagent dispensing part comprising a plurality of reagent needles, a guide assembly for guiding the plurality of reagent needles to move linearly, and a second driving assembly for driving the plurality of reagent needles to move linearly along the guide assembly, the guide assemblies being arranged in a direction determined by indexing in reagent sucking positions and reagent adding units of the processing units corresponding to the reagent dispensing parts, so that the reagent needles suck reagent from the reagent sucking positions and discharge the reagent into the reagent adding indexed reaction cups of the processing units corresponding to the reagent dispensing parts, and

And the dispatching component is used for dispatching the reaction cups which are positioned in the sample adding position and used for completing sample adding into each processing unit according to the detection flow.

According to a third aspect, an embodiment provides a sample analysis method comprising the steps of:

a reaction cup loading step of controlling a reaction cup loading part to supply and carry an empty reaction cup;

A sample feeding step of controlling a sample feeding part to dispatch a sample rack carrying samples to a sample sucking position;

a sample dispensing step of controlling a sample dispensing part to draw a sample from a sample suction position and dispense the sample into a reaction cup;

A first reagent dispensing step of controlling at least one of two reagent needles on the first reagent dispensing part to suck the first reagent in the reagent container through the first reagent sucking position and to make linear motion between the first reagent sucking position and the reagent adding transposition of the reaction part so as to dispense the first reagent to the reagent cup with the reagent adding transposition of the reaction part;

An incubation step, namely controlling a dispatching component to dispatch the reaction cup with the first reagent dispensing to the reaction component for incubation, and dispatching the reaction cup after incubation to a reagent adding component of a measuring component for transposition;

A second reagent dispensing step of controlling the rotation of the reagent carrying member so that the reagent container carrying the second reagent is positioned at a second reagent sucking position, controlling at least one of the two reagent needles on the second reagent dispensing member to suck the second reagent in the reagent container through the second reagent sucking position, and performing linear motion between the second reagent sucking position and the reagent adding position of the measuring member so as to dispense the second reagent to the reagent cup with the reagent adding position of the measuring member;

and a measuring and recycling step, wherein the control dispatching component dispatches the reaction cup with the second reagent dispensing to the measuring component for project detection, and dispatches the reaction cup after detection to the waste recycling device.

Drawings

FIG. 1 is a schematic diagram of a sample analyzer according to an embodiment;

FIG. 2 is a schematic diagram of a sample analyzer according to another embodiment;

FIG. 3 is a schematic view of a sample analyzer according to yet another embodiment;

FIGS. 4 (a) and 4 (b) are schematic structural views of reagent carrying members of two embodiments;

FIG. 5 is a schematic view of the structure of a reagent carrying component according to another embodiment;

FIG. 6 is a schematic view of a sample analyzer according to yet another embodiment;

FIG. 7 is a schematic view showing the structure of a reagent dispensing part according to an embodiment;

FIG. 8 is a schematic view showing the structure of a reagent dispensing part according to another embodiment;

FIG. 9 is a schematic view showing the structure of a reagent dispensing part according to still another embodiment;

FIG. 10 is a schematic view showing the structure of a reagent dispensing part according to still another embodiment;

FIG. 11 is a schematic view of a sample analyzer according to yet another embodiment;

Fig. 12 (a) is a schematic structural view of a transfer member of an embodiment, fig. 12 (b) is a schematic structural view of a first transfer member of an embodiment, fig. 12 (c) is a schematic structural view of a second transfer member of an embodiment, and fig. 12 (d) is a schematic structural view of a third transfer member of an embodiment;

FIG. 13 is a schematic view of the structure of a handle of an embodiment;

FIG. 14 is a schematic view showing the structure of a sample analyzer according to still another embodiment;

FIG. 15 is a schematic view of a sample analyzer according to yet another embodiment;

FIG. 16 is a schematic view showing the structure of a cleaning member according to an embodiment;

FIG. 17 is a flow chart of a sample analysis method according to an embodiment;

FIG. 18 is a timing diagram of two reagent needles of the same set of one embodiment;

FIG. 19 is a timing diagram of a two reagent needle of the same set of yet another embodiment;

FIG. 20 is a timing diagram of a two reagent needle of the same set of yet another embodiment;

FIG. 21 is a timing diagram of a two reagent needle of the same set of yet another embodiment;

FIG. 22 is a method of a sample analysis device of an embodiment.

Detailed Description

The invention will be described in further detail below with reference to the drawings by means of specific embodiments. Wherein like elements in different embodiments are numbered alike in association. In the following embodiments, numerous specific details are set forth in order to provide a better understanding of the present invention. However, one skilled in the art will readily recognize that some of the features may be omitted, or replaced by other elements, materials, or methods in different situations. In some instances, related operations of the present invention have not been shown or described in the specification in order to avoid obscuring the core portions of the present invention, and may be unnecessary to persons skilled in the art from a detailed description of the related operations, which may be presented in the description and general knowledge of one skilled in the art.

Furthermore, the described features, operations, or characteristics of the description may be combined in any suitable manner in various embodiments. Also, various steps or acts in the method descriptions may be interchanged or modified in a manner apparent to those of ordinary skill in the art. Thus, the various orders in the description and drawings are for clarity of description of only certain embodiments, and are not meant to be required orders unless otherwise indicated.

The numbering of the components itself, e.g. "first", "second", etc., is used herein merely to distinguish between the described objects and does not have any sequential or technical meaning. The term "coupled" as used herein includes both direct and indirect coupling (coupling), unless otherwise indicated.

The structure of the sample analysis device according to some embodiments of the present invention will be described below.

A sample analysis device is an instrument for analyzing and measuring a sample. Taking a coagulation analyzer as an example, a test flow of a sample analyzer will be described. The test flow of the coagulation analyzer is generally as follows, sample addition of a sample and reagent is completed in a reaction cup to prepare a reaction solution, after the reaction solution is uniformly mixed and incubated, the reaction cup is placed in a measurement component, the measurement component can irradiate multi-wavelength light to the reaction solution in the reaction cup, and a coagulation reaction curve of the reaction solution changing along with time is obtained by analysis of a coagulation method, an immunoturbidimetry method or a chromogenic substrate method, so that the coagulation time or other coagulation related performance parameters of the reaction solution are further calculated. In order to obtain a coagulation reaction curve of a reaction solution obtained by detection over time in a coagulation analyzer, the time required for adding a sample and a reagent, the incubation time, and the like in a test flow must be strictly set and controlled in order to obtain a correct measurement result.

Fig. 1 is a schematic structural diagram of a sample analyzer according to some embodiments of the invention. Sample analysis devices of some embodiments of the present invention may include a housing 1, a cuvette loading section 10, a sample section 20, a sample dispensing section 30, a reagent carrying section 40, one or more reagent dispensing sections 60, one or more processing units 50, and a scheduling section 70. It should be noted that, fig. 1 shows an example having two reagent dispensing parts 60 and two processing units 50, but it will be understood by those skilled in the art that this is only an example, and is not intended to limit the number of reagent dispensing parts 60 and processing units 50 to only two. The components of the sample analyzer will be described in detail below.

The housing 1 is an instrument housing of a sample analysis device, which may have, for example, a substantially rectangular parallelepiped or square box shape, and may function to house some components of the sample analysis device. For example, in some embodiments, the chassis 1 includes a first side 1a along a first direction and a second side 1b along a second direction.

The first direction and the second direction referred to herein, in some embodiments, may be perpendicular, e.g., the first direction is the Y direction in the figure and the second direction is the X direction in the figure.

The cuvette loading section 10 is used for supplying and carrying empty cuvettes. In the working process of the sample analysis device, the empty reaction cup is required to be used continuously to finish each test item, and the sample analysis device is used for preparing, incubating and measuring a reaction liquid by adding a sample and a reagent into the empty reaction cup so as to obtain a test result of the item. The cuvette loading section 10 may load an empty cuvette to a predetermined position, and the sample dispensing mechanism sucks a sample from the sample section 20 and discharges the sample into the empty cuvette at the predetermined position.

The sample member 20 is for supplying a sample rack carrying samples to be tested. In some embodiments, the sample member 20 may be disposed within the housing 1. There are a number of ways in which the sample section 20 can be implemented.

In one implementation of the sample part 20, the sample part 20 may be a sample introduction part 21, and the sample introduction part 21 is used for dispatching a sample rack carrying samples to a sample suction position. Fig. 2 is an example, and the sample introduction part 21 may include a loading region 21a, a sample introduction channel 21b, and an unloading region 21c, wherein the sample introduction channel 21b may be provided with a sample suction position 21d. In the figure, the X direction and the Y direction are vertical, the X1 direction and the X2 direction are opposite directions, and the Y1 direction and the Y2 direction are opposite directions. The user can place the sample rack that bears the sample to be tested in the loading area 21a, the loading area 21a moves the sample rack in the Y1 direction in the figure to enter the sample introduction channel 21b, the sample rack can move along the X1 direction in the sample introduction channel 21b and pass through the sample suction position, the sample on the sample rack can be sucked by the sample dispensing component 30 when passing through the sample suction position, the sample rack enters the unloading area 21c from the sample introduction channel 21b along the Y2 direction, and the user can take out the sample rack from the unloading area 21 c. The sample injection part 21 is suitable for a large number of sample testing occasions, the sample injection part 21 can be arranged independently of the sample analysis device, and when the sample analysis device needs to be connected into a pipeline type testing system, the sample injection part 21 can be directly removed.

In another implementation of the sample member 20, the sample member 20 may be a sample placement area 22, the sample placement area 22 being for placing a sample rack carrying samples to be tested. Fig. 3 is an example. The sample placement area 22 may have a plurality of channels 22a, each channel 22a may be configured to place a sample rack, a user may push the sample rack into the channel 22a in the Y1 direction in the drawing, the sample dispensing member 30 may sequentially aspirate samples from the sample racks in each channel 22a, and after all of the samples from the sample racks have been aspirated, the user may pull the sample rack out of the channel 22a in the Y2 direction in the drawing. The sample placement area 22 does not require sample racks to be dispatched, thus occupying a small volume, contributing to downsizing of the sample analysis device, and contributing to a miniaturized design of the sample analysis device.

The sample dispensing member 30 is used to aspirate a sample from a sample aspiration site and discharge the sample into a cuvette at a sample application site. In some embodiments, the sample dispensing member 30 may be disposed within the housing 1. In some embodiments, the sample dispensing mechanism 30 may include a sample needle that is driven for movement in two or three dimensions by a two or three dimensional drive mechanism. In some embodiments, the sample needle may be one or more. In order to simplify the movement track and reduce the volume and size of the sample analyzer, the sample sucking position and the predetermined position to which the cuvette loading unit 10 loads the empty cuvette may be designed to be on a straight line, for example, on a straight line along the first direction, so that the sample needle only needs to reciprocate between the sample sucking position and the predetermined position in the first direction, which not only increases the movement speed of the sample needle, but also is beneficial to reducing the size of the sample analyzer, and is beneficial to the miniaturization design of the sample analyzer.

The reagent carrier 40 is used for carrying a reagent, for example the reagent carrier 40 may have a plurality of positions for carrying reagent containers for carrying reagents. Generally, the reagent carrying apparatus 40 may provide a cooling function or the like for the carried reagent, thereby securing the activity of the reagent. In some embodiments, the reagent carrier 40 may be disposed within the chassis 1. In some embodiments, the reagent carrier 40 is provided in a disc-like structure having a plurality of positions for carrying reagent containers, the reagent carrier 40 being capable of rotating and transporting the reagent containers carried thereby to rotate the reagent containers to a reagent sucking position for the reagent dispensing member 60 to suck reagent, e.g. the reagent carrier 40 comprises a first drive assembly for driving rotation thereof, the first drive assembly driving rotation of the reagent carrier 40 for rotating the reagent containers to the reagent sucking position. The reagent carrying member 40 provided in a disk-like structure will be described in detail below.

Referring to fig. 4 (a), in some embodiments, the reagent carrying member 40 is configured in a disc-shaped structure, and has a plurality of positions for placing reagent cups 41, each reagent cup 41 includes one or more cavities for containing reagents required for testing items, one reagent is placed in each cavity, and the reagent carrying member 40 includes a first driving component for driving the reagent carrying member 40 to rotate, so as to rotate the cavities of the reagent cups 41 containing the reagents required for the items to corresponding reagent sucking positions. In one example, the reagent cup 41 comprises at least a first cavity 41a for carrying a first reagent and a second cavity 41b for carrying a second reagent, for example, the reagent cup 41 comprises at least a first cavity 41a for carrying a mixed reagent R1 and a second cavity 41b for carrying a trigger reagent R2, the reagent carrying member 40 comprises a first reagent sucking position and a second reagent sucking position different from the first reagent sucking position, the first driving assembly drives the reagent carrying member 40 to rotate and drives the reagent cup 41 to rotate so as to rotate the first cavity 41a of the reagent cup 41 to the first reagent sucking position, and the first driving assembly drives the reagent carrying member 40 to rotate and drives the reagent cup 41 to rotate so as to rotate the second cavity 41b to the second reagent sucking position.

Referring to fig. 4 (b), in some embodiments, the reagent carrying member 40 is configured in a disc-shaped structure having a plurality of positions for carrying a first reagent container 42 and a plurality of positions for carrying a second reagent container 43. The reagent carrier 40 comprises a first driving assembly for driving the reagent carrier 40 to rotate and driving the first reagent container 42 to rotate so as to rotate the first reagent container 42 to the first reagent sucking position, and the first driving assembly drives the reagent carrier 40 to rotate and driving the second reagent container 43 to rotate so as to rotate the second reagent container 43 to the second reagent sucking position. In one example, the reagent carrier 40 may comprise a plurality of independently rotatable tracks. For example, the reagent carrier 40 may comprise two ring tracks, an inner ring track and an outer ring track, on which a plurality of first reagent containers 42 may be positioned, and correspondingly, on which a plurality of second reagent containers 43 may be positioned, the inner ring and outer ring tracks being driven to rotate independently by the first drive assembly.

While two types of reagent carrying members 40 have been described above, for example, fig. 4 (a) is an example of placing the reagent cup 41, fig. 4 (b) is an example of realizing the reagent carrying member 40 by a plurality of tracks capable of rotating independently, and as will be understood by those skilled in the art, the two types of reagent carrying members 40 may be realized by a plurality of tracks capable of rotating independently, and at least one track or each track has a plurality of positions for placing the reagent cup 41, for example, fig. 5 is an example, the reagent carrying member 40 may include two tracks, an inner track and an outer track, and a plurality of positions for placing the reagent cup 41 may be provided on the outer track, and accordingly, a plurality of positions for placing the reagent cup 41 may be provided on the inner track, and the inner track and the outer track may be driven to rotate independently by the first driving assembly.

The above are some of the descriptions of the reagent carrying section 40. The reagent carrier 40 may rotate and dispense the respective reagent required for the test item to the reagent aspirating position corresponding to the reagent dispensing member 60 by rotating during the working cycle, for example, a first reagent to a first reagent aspirating position and a second reagent to a second reagent aspirating position.

The processing unit 50 is used for receiving a cuvette carrying a sample and processing the sample of the cuvette. The sample herein refers to a reaction solution composed of a sample and a reagent. The processing unit 50 may be one or more.

Referring to fig. 6, in some embodiments, at least one of the processing units 50 is a reaction component 51 for incubating a sample, and the reaction component 51 is used for carrying a cuvette and incubating the sample in the cuvette. In some embodiments, the reaction block 51 is rectangular in shape with a plurality of reaction cup placement sites. Typically, the reaction part 51 may heat the reaction solution or the sample in the cuvette placed on each cuvette to incubate the sample, e.g. the sample in the cuvette is heated and kept at 37±0.5 ℃, and the specific heating time and the heating temperature may be determined by the heating parameters corresponding to the different test items. In some embodiments, the length direction of the reaction member 51 is arranged along a first direction, for example, along the Y direction in the drawing.

In some embodiments, at least one of the processing units 50 is a measuring part 52 for measuring a sample, the measuring part 52 is used for carrying a cuvette and detecting the sample in the cuvette, and in some embodiments, the measuring part 52 is rectangular and has a plurality of positions for placing the cuvette. In general, the measuring unit 52 may be provided with a single detecting unit (not shown) for each cuvette placement site, and each detecting unit is configured to detect a sample in a cuvette placed in the corresponding cuvette placement site. In some embodiments, the length direction of the measurement member 52 is disposed in a second direction different from the first direction, such as along the X direction in the figures.

In some embodiments, the reaction component 51 and the assay component 52 are disposed in an adjacent manner around the reagent carrying component 40. In particular embodiments, the reaction member 51 and the assay member 52 are disposed along the first side 1a and the second side 1b, respectively, and surround the reagent carrier 40 in an adjacent manner.

The rectangular reaction part 51 and the measuring part 52 are disposed along the first side 1a and the second side 1b, respectively, and surround the reagent carrying part 40 in an adjacent manner, so that space can be saved, the size of the sample analyzing apparatus can be reduced, and the reagent carrying part 40 can also interact with the reaction part 51 and the measuring part 52 through the reagent dispensing part 60.

In some embodiments, sample components 20, such as sample introduction component 21, cuvette loading component 10, reaction component 51, and assay component 52, are disposed about reagent carrier component 40. The application centers on the reagent bearing component 40, designs the dispatching track of the whole detection flow of the reaction cup around the reagent bearing component 40, has novel design and saves space.

Each processing unit 50 may be provided with a respective reagent-adding index, for example, the reaction part 51 is provided with at least one incubation index 51a for placing the reaction cups, the number of the incubation index 51a may be one or more, and when the position of the incubation index 51a for placing the reaction cups is set to 1, the incubation index 51a may be set in a position-adjustable manner so that the reaction cups placed on the incubation index 51a can be positionally corresponding to the respective reagent needles in the first reagent-dispensing part (the first reagent-dispensing part corresponds to the reaction part for adding reagent to the reaction cups in the reaction part) to receive the reagents dispensed by the respective reagent needles. In some embodiments, an in-incubation index 51a is disposed between the reagent carrying component 40 and the reaction component 51. The assay component 52 is configured with at least one in-assay index 52a for placement of a cuvette, the number of in-assay indexes 52a may be one or more, and in some embodiments, in-assay indexes 52a are disposed between the reagent carrier component 40 and the assay component 52. When the position of the in-assay index 52a where the cuvette is placed is set to 1, the in-assay index 52a may be set in a position-adjustable manner so that the cuvette placed on the in-assay index 52a can be positionally corresponding to each reagent needle in the second reagent dispensing part (the second reagent dispensing part corresponds to the measuring part, adding reagent to the cuvette in the measuring part) to receive the reagent dispensed by each reagent needle.

Shown in fig. 6 is one number of in-incubation indexes 51a, each in-incubation index 51a having two placement positions for reaction cups, e.g. a first position and a second position for placement of reaction cups, and one number of in-assay indexes 52a, each in-assay index 52a having two placement positions for reaction cups, e.g. a third position and a fourth position for placement of reaction cups.

The reagent dispensing part 60 is used to aspirate the reagent from the reagent sucking site and discharge it into the cuvette at the reagent adding site. For example, the reagent dispensing component 60 can aspirate a first reagent from a first reagent aspirating position referred to herein and discharge it into a reaction cup, and the reagent dispensing component 60 can aspirate a second reagent from a second reagent position referred to herein and discharge it into a reaction cup. In some embodiments, reagent dispensing component 60 may be disposed within enclosure 1.

The reagent dispensing member 60 may be implemented by a reagent needle. Thus, in some embodiments, the reagent dispensing component 60 comprises a reagent needle for aspirating reagent from the reagent carrier component 40 and discharging into the reaction cup.

From the perspective of the number of reagent needles, in some embodiments, reagent dispensing component 60 may have a plurality of reagent needles, each of which is disposed in a manner that enables independent movement relative to the other. The reagent needles may in particular be configured in that each processing unit 50 is configured with a set of reagent needles for sucking up reagent from the reagent carrier 40 and discharging it into the reaction cups of the respective processing unit 50, and each set of reagent needles comprises at least two reagent needles. For example, a set of reagent needles may be provided for the reaction member 51, and a set of reagent needles may be provided for the measurement member 52. In particular embodiments, the reaction part 51 may be provided with a first set of reagent needles arranged in a linear motion between the reagent sucking position and the incubation index 51a, the first set of reagent needles being arranged to suck reagent from the reagent sucking position and discharge it into the reaction cup located at the incubation index 51a, the first set of reagent needles comprising at least one reagent needle, and similarly the measurement part 52 may be provided with a second set of reagent needles arranged in a linear motion between the reagent sucking position and the measurement index 52a, the second set of reagent needles being arranged to suck reagent from the reagent sucking position and discharge it into the reaction cup located at the measurement index 52a, the second set of reagent needles comprising at least one reagent needle.

From the perspective of the number of reagent dispensing members 60, in some embodiments, the number of reagent dispensing members 60 is equal to the number of processing units 50, and one reagent dispensing member 60 corresponds to one processing unit 50. Fig. 1 to 3 above are all examples of this. Specifically, there may be two reagent dispensing parts 60, one of the reagent dispensing parts 60 corresponds to the reaction part 51, and the other reagent dispensing part 60 corresponds to the measurement part 52. Each processing unit 50 is provided with a reagent dispensing component 60, so that the action of the reagent adding flow of the detection item is decomposed, namely, each reagent dispensing component 60 only needs to add corresponding reagents for the reaction cups of the corresponding processing units 50, so that the sample adding of the corresponding reagents of the detection item is completed by division, and the efficiency is improved.

The specific structure of the reagent dispensing member 60 will be described below.

Referring to fig. 7, each reagent dispensing part 60 includes a plurality of reagent needles 61 and a guide assembly 62 for guiding the plurality of reagent needles 61 to move linearly, and a second driving assembly 63 for driving the plurality of reagent needles 61 to move linearly along the guide assembly 62. The guide member 62 is disposed in a direction determined by the reagent sucking position and the reagent transferring position of the processing unit 50 corresponding to the reagent dispensing part 60 so that the reagent needle 61 sucks the reagent from the reagent sucking position and discharges the reagent into the reagent cup of the reagent transferring position of the processing unit 50 corresponding to the reagent dispensing part 60. For example, the reagent dispensing part 60 on the left in fig. 7 is provided with a guide member 62 in a direction determined by the reagent sucking position and the in-incubation position 51a of the reaction part 51 so that the reagent needle 61 of the reagent dispensing part 60 sucks the reagent from the reagent sucking position and discharges the reagent into the reaction cup located in the in-incubation position 51a, and the reagent dispensing part 60 on the right in fig. 7 is provided with a guide member 62 in a direction determined by the reagent sucking position and the in-measurement position 52a of the measurement part 52 so that the reagent needle 61 of the reagent dispensing part 60 sucks the reagent from the reagent sucking position and discharges the reagent into the reaction cup located in the in-measurement position 52 a. In some embodiments, the number of second drive assemblies 53 of each reagent dispensing component 60 is equal to the number of reagent needles 61, and the respective independent drive force outputs of the plurality of second drive assemblies 53 act on the plurality of reagent needles 61 to drive the plurality of reagent needles 61 independently of one another in a linear motion along the guide assembly 52 between reagent sucking and reagent feeding positions. For example, the example shown in fig. 7 is an example in which each reagent dispensing part 60 includes two reagent needles 61, and each reagent needle 61 is independently driven by a respective second driving assembly 63.

The guide assembly 52 can be implemented in a variety of ways, to name a few.

Fig. 8 is a schematic view of the side of the reagent dispensing component 60. In some embodiments, the guide assembly 62 of each reagent dispensing member 60 includes a cross member 62a and a plurality of guide members 62b disposed in parallel along the length of the cross member 62a, the cross member 62a being disposed in a direction determined by the reagent sucking position and the indexing of the reagent in the processing unit 50 of the corresponding reagent dispensing member 60, the number of guide members 62b being equal to the number of reagent needles 61 of the reagent dispensing member 60, and the plurality of reagent needles 61 being slidably connected to the plurality of guide members 62b, respectively, such that the reagent needles 61 move linearly along the guide members 62b between the reagent sucking position and the indexing of the reagent in the reagent. In some embodiments, two reagent needles 61 of each reagent dispensing component 60 are provided, two guide pieces 62b of each reagent dispensing component 60 are provided, the two guide pieces 62b are all linear guide rails, the two linear guide rails are respectively arranged on two sides of the cross beam 62a along the long axis direction of the cross beam 62a, namely, a schematic diagram of one side of the cross beam 62a is shown in fig. 8, one reagent needle 61 is exposed on the other side of the cross beam, the two reagent needles 61 are respectively arranged on the linear guide rails on two sides of the cross beam 62a and are in sliding connection with the linear guide rails, and the reagent needle 61 moves linearly along the linear guide rail where the reagent needle 61 is positioned between reagent sucking position and reagent adding position.

This is an embodiment in which one reagent dispensing member is realized by providing one reagent needle on each side of one cross beam. In other embodiments, a reagent dispensing component may be implemented by two parallel beams, each beam having only one reagent needle, as described in more detail below.

Referring to fig. 9 and 10, in some embodiments, the guide assembly 62 of each reagent dispensing member 60 includes a plurality of parallel beams 62a and a plurality of guide members 62b respectively disposed on the plurality of beams 62a and along a length direction of the beams 62a, the plurality of beams 62a are disposed along a direction determined by reagent sucking positions and indexing positions of reagents in the processing unit 50 of the corresponding reagent dispensing member 60, the number of the guide members 62b is equal to the number of the reagent needles 61 of the reagent dispensing member 60, and the plurality of reagent needles 61 are slidably connected to the plurality of guide members 62b, respectively, such that the reagent needles 61 move linearly along the guide members 62b between the reagent sucking positions and the reagent indexing positions. In some embodiments, the plurality of guide members 62b of each reagent dispensing component 60 are linear guide rails, the plurality of linear guide rails are respectively arranged along the long axis direction of the plurality of cross beams 62a, the plurality of reagent needles 61 are respectively arranged on the linear guide rails of the plurality of cross beams 62a and are in sliding connection with the linear guide rails, and the reagent needles 61 perform linear motion between reagent sucking positions and reagent adding positions along the linear guide rails where the reagent needles are positioned.

In some embodiments, the cross beam 52a of the guide assembly 52 in each reagent dispensing component 60 is fixedly disposed in an upper position of reagent sucking and indexing in the reagent of the processing unit 50 of the corresponding reagent dispensing component 60.

The reagent dispensing component 60 realizing the multi-needle linear motion through the beam structure does not occupy too much space of the motion track of the reagent needle 61 and reduces the layout interference to other components as much as possible, so that the structure of the sample analysis device can be more compact, and the miniaturization design of the sample analysis device is very facilitated.

In some embodiments, the reagent needles 61 of the different reagent dispensing parts 60 do not intersect each other along a straight line, so that the movement of the reagent needles 61 of the different reagent dispensing parts 60 is not interfered with each other, which is advantageous for improving the test speed.

In some embodiments, there is at least one processing unit 50 that indexes reagent containing sites that include the same number of reagent pins 61 as the reagent dispensing component 60 corresponding to that processing unit 50. For example, in the example of FIG. 6, the reagent-adding index of the reaction part 51 is the incubation index 51a in the figure, two reaction cups may be placed in the incubation index 51a, and the number of reagent needles 61 of the reagent dispensing part 60 corresponding to the reaction part 51 is two. In fig. 6, the reagent-adding index of the measuring unit 52 is the index 52a in the drawing, and two reaction cups may be placed in the index 52a, and the number of reagent needles 61 of the reagent dispensing unit 60 corresponding to the measuring unit 52 is two. In some embodiments, the number of reagent sucking sites is the same as the number of reagent needles. For example, in fig. 6, there are two reagent dispensing parts 60, and each reagent dispensing part 60 has two reagent needles 61, so that the number of reagent sucking sites is four. The number of the reagent needles is the same as the number of the reagent adding positions, so that each reagent needle can be clearly divided into work, and reagent is added to the reaction cups on the respective reagent adding positions, thereby being beneficial to improving the test speed.

In some embodiments, reagent needles 61 in the same reagent dispensing component 60 are used to aspirate the same type of reagent. For example, the reagent needles 61 of the reagent dispensing part 60 corresponding to the reaction part 51 are all used to aspirate the first reagent, and the reagent needles 61 of the reagent dispensing part 60 corresponding to the measurement part 52 are all used to aspirate the second reagent. The different reagent dispensing members 60 are used to aspirate different reagents, which makes each reagent dispensing member 60 work clearly, which is advantageous for improving the test speed.

In some embodiments, each reagent needle 61 of the reagent dispensing part 60 is provided with a heating part (not shown) for heating the reagent sucked by the reagent needle. Since each reagent dispensing member 60 includes a plurality of reagent needles 61, two reagent needles may be taken as an example, and each reagent needle 61 is provided with a heating member, and since there are two reagent needles 61, each reagent needle 61 has a sufficient time to heat the sucked reagent, for example, a double time, while maintaining the original speed, so that the temperature of the reagent has been relatively close to a predetermined temperature when the reagent reaches the corresponding processing unit 50, that is, the reagent has been sufficiently preheated.

The above is some description of the reagent dispensing component 60. The following describes the matching relationship and the corresponding configuration between the reagent dispensing member 60 and the processing unit 50, taking the case where the number of the processing units 50 is two, and specifically the reaction member 51 and the measurement member 52.

In some embodiments, the reagent dispensing part 60 corresponding to the reaction part 51 is a first reagent dispensing part, and the reagent dispensing part 60 corresponding to the measurement part 52 is a second reagent dispensing part, which will be described in detail below.

In some embodiments, the first reagent dispensing member 60 comprises a first cross member 6a and a first set of reagent needles comprising at least a plurality of first reagent needles 6b, e.g., two, the plurality of first reagent needles 61 being disposed on the first cross member 62a and being linearly movable in the longitudinal direction of the first cross member 62a to aspirate a first reagent from a first reagent aspirating position and discharge into a reaction cup located in the in-incubation index 51 a. In some embodiments, the first beam 62a is disposed along a direction defined by the first reagent sucking position and the in-incubation index 51a, and the first beam 62a is fixedly disposed at an upper position corresponding to the first reagent sucking position and the in-incubation index 51 a.

In some embodiments, the first beam 62a of the first reagent dispensing part 60 is provided as one, and the plurality of first reagent needles 61 are provided in parallel on the first beam 62a and linearly move along the long axis direction of the first beam 62 a. Taking the example that the first group of reagent needles has two first reagent needles 61, two linear guide rails are respectively arranged on two sides of the first cross beam 62a along the long axis direction, the two first reagent needles 61 are respectively arranged on the linear guide rails on two sides of the first cross beam 62a, and the first reagent needles 61 do linear motion between the first reagent sucking position and the rotating position 51a in incubation along the linear guide rails. This is an embodiment in which the first reagent dispensing member is realized by providing a first reagent needle on each side of a first beam.

In some embodiments, the number of the first beams 62a of the first reagent dispensing part 60 is equal to the number of the plurality of first reagent needles 61 of the first group of reagent needles, each of the first beams 62a is provided with one first reagent needle 61, and the first beams 62a are arranged in parallel. In some specific embodiments, each of the plurality of first cross members 62a is provided with a linear guide along the long axis direction thereof, and the plurality of first reagent needles 61 are respectively provided on the linear guide of the plurality of first cross members 62a for performing linear movement between the first reagent sucking position and the incubation indexing position 51 a. This is an embodiment of the first reagent dispensing member by means of a plurality of, for example, two parallel first beams, each of which is provided with only one first reagent needle.

In some embodiments, the first reagent dispensing component 60 further includes a plurality of driving mechanisms, such as second driving components, for driving the plurality of first reagent needles to move linearly independently of each other, where the number of the plurality of second driving components is equal to the number of the plurality of first reagent needles, and each independent driving force output end of the plurality of second driving components acts on the plurality of first reagent needles respectively, so as to drive the plurality of first reagent needles to move linearly along the long axis direction of the first beam between the first reagent sucking position and the incubation middle position 51 a.

Although the above incubation index 51a includes the first position and the second position for placing the cuvette, in the case where the first reagent dispensing section 60 has two first reagent needles 61, one of the first reagent needles 61 moves linearly along the first beam 62a between the first reagent sucking position and the first position, and the other first reagent needle 61 moves linearly along the first beam 62a between the first reagent sucking position and the second position.

In some embodiments, the first reagent dispensing component 60 further comprises first Z-direction driving components 64 for driving the first reagent needles 61 in the first group of reagent needles to move in the vertical direction, wherein the number of the first Z-direction driving components 64 is the same as that of the first reagent needles 61 in the first group of reagent needles, each first Z-direction driving component 64 comprises a first Z-direction guide 64a for guiding the first reagent needles 61 to move in the vertical direction and a first Z-direction driving component 64b for driving the first reagent needles to move along the first Z-direction guide, and the first reagent needles 61 are in sliding connection with the first cross beam 62a through the first Z-direction guide 64a and the first Z-direction driving component 64b, so that the first reagent needles 61 can move in the vertical direction relative to the first cross beam 62a under the driving of the first Z-direction driving component 64 b. As will be appreciated by those skilled in the art, in the case of reciprocating linear motion of the reagent needle, it is necessary to move in a vertical direction when reaching each position to perform the operations of sucking and discharging the reagent.

In some embodiments, the first reagent needle 61 may be further provided with a heating means (not shown) for heating the reagent sucked thereby.

The first reagent dispensing member 60 is described above, and the second reagent dispensing member 60 is described below.

The second reagent dispensing member 60 includes a second cross member 62a and a second group of reagent needles including at least a plurality of second reagent needles 61, for example, two, and the plurality of second reagent needles 61 are provided on the second cross member 62a and linearly move in the longitudinal direction of the second cross member 62a to suck the second reagent from the second reagent sucking position and discharge the second reagent into the cuvette at the measurement center position 52 a. In some embodiments, the second beam 62a is disposed along a direction defined by the second reagent-sucking position and the in-assay index 52a, and the second beam 62a is fixedly disposed at an upper position corresponding to the second reagent-sucking position and the in-assay index 52 a.

In some embodiments, the second beam 62a of the second reagent dispensing part 60 is provided as one, and the plurality of second reagent needles 61 are provided in parallel on the one second beam 62a and linearly move in the longitudinal direction of the second beam 62 a. Taking the example that the second group of reagent needles has two first reagent needles 61, two linear guide rails are respectively arranged on two sides of the second cross beam 62a along the long axis direction, two second reagent needles 61 are respectively arranged on the linear guide rails on two sides of the second cross beam 62a, and the second reagent needles 61 do linear motion between the second reagent sucking position and the measuring index 52a along the linear guide rail. This is an embodiment in which the second reagent dispensing member is realized by providing a second reagent needle on each side of a second beam.

In some embodiments, the number of the second beams 62a of the second reagent dispensing part 60 is equal to the number of the plurality of second reagent needles 61 of the second group of reagent needles, and each of the second beams 62a is provided with one second reagent needle 61, and two of the second beams 62a are disposed parallel to each other. In some specific embodiments, the plurality of second cross members 62a are each provided with a linear guide along the long axis direction thereof, and the plurality of second reagent needles 61 are respectively provided on the linear guides of the plurality of second cross members 62a for performing linear movement of the second reagent needles 61 between the second reagent sucking position and the measurement center index 52 a. This is an embodiment of the second reagent dispensing member by means of a plurality of, for example, two parallel second beams, each of which is provided with only one second reagent needle.

In some embodiments, the second reagent dispensing component 60 further includes a plurality of driving mechanisms that are different from the second driving assemblies and independently drive the plurality of second reagent needles to perform linear motion, for example, a third driving assembly, where the number of third driving assemblies is equal to the number of the plurality of second reagent needles, and each independent driving force output end of the plurality of third driving assemblies acts on the plurality of second reagent needles respectively, so as to drive the plurality of second reagent needles to perform linear motion between the second reagent position and the measurement transfer position along the long axis direction of the second beam. The second drive assembly and the third drive assembly may be identical in structure.

In the case where the index 52a for measurement includes the third position and the fourth position for placing the cuvette, for example, in the above description, in the case where the second reagent dispensing part 60 has two second reagent needles 61, one of the second reagent needles 61 moves linearly along the second beam 62a between the second reagent sucking position and the third position, and the other of the second reagent needles 61 moves linearly along the second beam 62a between the second reagent sucking position and the fourth position.

In some embodiments, the second reagent dispensing component 60 further comprises a second Z-direction driving component 64 for driving the second reagent needles 61 in the second group of reagent needles to move in the vertical direction, the number of the second Z-direction driving components 64 is the same as that of the second reagent needles 61 in the second group of reagent needles, the second Z-direction driving components 64 comprise a second Z-direction guide piece 64a for guiding the second reagent needles 61 to move in the vertical direction and a second Z-direction driving piece 64b for driving the second reagent needles 61 to move along the second Z-direction guide piece 64a, and the second reagent needles 61 are in sliding connection with the second cross beam 62a through the second Z-direction guide piece 64a and the second Z-direction driving piece 64b, so that the second reagent needles 61 can move in the vertical direction relative to the second cross beam 62a under the driving of the second Z-direction driving piece 64 b.

In some embodiments, the second reagent needle 61 may be further provided with a heating means (not shown) for heating the reagent sucked thereby.

In some embodiments, the first reagent dispensing member 60 has a plurality of first reagent needles 61 having a linear motion trajectory between the first reagent sucking position and the in-incubation index 51a that is a first motion trajectory, and the second reagent dispensing member 60 has a plurality of second reagent needles 61 having a linear motion trajectory between the second reagent sucking position and the in-assay index 52a that is a second motion trajectory, wherein the first motion trajectory does not intersect with the second motion trajectory.

The first reagent dispensing member 60 and the second reagent dispensing member 60 may have the same structure, except that the directions in which they are disposed are different, the first reagent dispensing member 60 is disposed in the direction of the reaction member 51 for engagement with the reaction member 51, and the second reagent dispensing member 60 is disposed in the direction of the measurement member 52 for engagement with the measurement member 52.

The above is some description of the reagent dispensing component 60. The reagent dispensing part 60 is constructed in a beam structure such that the reagent needle 61 continuously makes a reciprocating linear motion between the reagent sucking position of the reagent carrying part 40 and the reagent adding position of the corresponding processing unit, thereby completing the sucking and discharging of the corresponding reagent.

The movements of the different reagent dispensing members 60 are independent and do not interfere with each other, thus playing an obvious role in space miniaturization of the instrument and in improvement of the test speed.

The dispatching unit 70 is used for dispatching the reaction cups, for example, the dispatching unit 70 dispatches the reaction cups with the finished sample in the sample adding position to each processing unit 50 according to the detection flow, for example, the dispatching unit 70 dispatches the reaction cups with the finished reagent, for example, the first reagent, on the incubation index 51a to the reaction unit 51, and dispatches the reaction cups with the finished reagent, for example, the second reagent, on the measurement index 52a to the measurement unit 52. The specific structure of the scheduling unit 70 will be described below.

Referring to fig. 11, in some embodiments, the dispatch component 70 includes a first transfer component 71, a second transfer component 73, and a third transfer component 75, and in order to mate the three transfer components 71, 73, and 75, in some embodiments, the sample analysis device is further provided with a first buffer index 77 and a second buffer index 78. In some embodiments, the first buffer index 77 may be designed with a fixed buffer bit, and only one cuvette may be placed, which is advantageous for reducing the volume and size of the sample analysis device, and similarly, the first buffer index 78 may be designed with a fixed buffer bit, and only one cuvette may be placed, which is advantageous for reducing the volume and size of the sample analysis device. Of course, in some embodiments, the first cache index 77 and the second cache index 78 may be configured with multiple cuvette placement bits when a fixed cache bit design is used, thereby enabling more cuvette placement bits to be scheduled. Even further, in some embodiments, the first in-buffer index 77 may be configured as a moving or rotating buffer position, for example, the first in-buffer index 77 may include a cuvette placement position that is driven to move or rotate, such that during the transfer of a cuvette to the first in-buffer index 77 by the first in-buffer index 77, the first in-buffer index 77 may also be controlled to move or rotate to a predetermined position to receive a cuvette transferred by the first in-buffer index 71, and in addition, the first in-buffer index 77 may be controlled to move or rotate to a predetermined position to enable the second in-buffer index 73 to more quickly grasp a cuvette on the first in-buffer index 77, such that during the transfer of a cuvette to the second in-buffer index 78 by the second in-buffer index member 71, the second in-buffer index 78 may be controlled to move or rotate to a predetermined position to also enable the second in-buffer index 78 to more quickly grasp a cuvette on the first in-buffer index 77, and further, such that the transfer of a cuvette to the second in-buffer index member 73 may be controlled to move or rotate to a predetermined position to more quickly grasp a cuvette on the first in-buffer index 77, such that the whole cuvette can be transferred to the second in-buffer index 78 is more quickly received by the second in-buffer index member 73 and the second in-buffer index 78 may be controlled to more quickly grasp a cuvette placement position.

There are various specific implementations of the first transferring member 71, the second transferring member 73 and the third transferring member 75, for example, a rail type transferring member for transferring the reaction cup by placing the reaction cup on a rail, a turntable type transferring member for transferring the reaction cup carried by the turntable to a corresponding position by placing the reaction cup on a turntable type structure and transferring the reaction cup by the turntable itself, a two-dimensional or three-dimensional driving mechanism for driving a gripper, for example, the first transferring member 71, the second transferring member 73 and the third transferring member 75 are implemented, the reaction cup is gripped by the gripper, and then the gripper is driven to move by the two-dimensional or three-dimensional driving mechanism, so that the reaction cup is transferred to the corresponding position.

Referring to fig. 12 (a), the first transfer member 71, the second transfer member 73, and the third transfer member 75 each include a cup gripping hand 79 and a driving member for driving the cup gripping hand 79 to move. In some embodiments, the handle 79 is used to hold a reaction cup, for example, FIG. 13 is a schematic view of the handle 79. The opening and closing of the cup grabbing hand 79 can be achieved jointly by a driving mechanism and a spring, the opening of the cup grabbing hand 79 can be driven to open by the driving mechanism, when the driving mechanism is not driven, the cup grabbing hand 79 is automatically closed through the spring and clamps a clamped object such as a reaction cup, and in some examples, a circle of protrusions which are suitable for clamping of the cup grabbing hand can be arranged on the reaction cup.

The transfer members and their functions will be described below.

The first transfer member 71 is configured to transfer the loaded cuvette to the first buffer memory for indexing 77. In some embodiments, the first transfer member 71 moves linearly in a first direction, e.g., the Y direction in the figure, and transfers the loaded cuvette to the first buffer memory for indexing 77. The first transfer component 71 moves along the straight line to move the reaction cup, so that the volume of the sample analysis device occupied in the process of transporting the reaction cup is relatively reduced, and the miniaturization design of the sample analysis device is facilitated.

Referring to fig. 14, in some embodiments, the sample analyzer may further include a sample loading station 10a, a pre-dilution station 10b, a first cup station 10c, and a second cup station 10d. In some embodiments, the first transfer member 71 may be movable in a first direction, such as the Y-direction in the figures, between the loading station 10a, the pre-dilution station 10b, the first cup station 10c, and the first buffer indexing 77. The loading site 10a may be a predetermined position to which the cuvette loading section 10 loads an empty cuvette, and in general, the sample dispensing section 30 sucks a sample from the sample sucking site and discharges the sample into the cuvette located at the loading site 10a to complete the loading. In some cases, the sample needs to be pre-diluted, so that the first transfer component 71 transfers the empty cuvette at the sample loading site 10a to the pre-dilution site 10b, the sample dispensing component 30 sucks the sample from the sample sucking site and discharges the sample into the cuvette at the pre-dilution site 10b, and then dilutes the sample in the cuvette at the pre-dilution site 10b, and in this process, the cuvette loading component 10 loads a new empty cuvette onto the sample loading site 10a, and then the sample dispensing component 30 sucks the pre-diluted sample from the cuvette at the pre-dilution site 10b and discharges the sample to the sample loading site 10a, thereby completing the sample loading, and the first transfer component 71 transfers the cuvette at the pre-dilution site 10b to the first cuvette throwing site 10c for example.

As described above, in some embodiments, the first transferring member 71 only needs to move in the first direction, so the driving member of the first transferring member 71 may be a two-dimensional driving member for driving the handle 79 of the first transferring member 71 to move in the first direction and the vertical direction, where the first direction may be the Y direction in the drawing and the vertical direction is the direction perpendicular to the paper surface in the drawing. Referring to fig. 12 (b), in some embodiments, the first transferring member 71 includes a first direction guide 71a, a first direction driving member 71b, a vertical direction guide 71c and a vertical direction driving member 71d, the vertical direction guide 71c is provided with a cup hand 79 of the first transferring member 71 in a sliding manner, and the cup hand 79 can move in the vertical direction along the vertical direction guide 71c by driving the vertical direction guide 71c, the first direction guide 71a is provided with a vertical direction guide 71c in a sliding manner, and the vertical direction guide 71c can move in the first direction along the first direction guide 71a by driving the first direction driving member 71b, so that the cup hand 79 of the first transferring member 71 is driven to move in the first direction, and by such a structure, the cup hand 79 of the first transferring member 71 can move in the first direction and the vertical direction in two dimensions. In particular embodiments, the first direction guide 71a may comprise a first rail, the first direction driving part 71b may comprise a first stepping motor, a first driven wheel and a first synchronous belt, the first synchronous belt is sleeved between the first stepping motor and the first driven wheel, the vertical direction guide 71c may be fixedly connected with the first synchronous belt, similarly, the vertical direction guide 71c may comprise a vertical rail, the vertical direction driving part 71d may comprise a lifting stepping motor and a vertical screw rod, and a mounting plate may be sleeved on the vertical screw rod in a threaded manner for mounting the cup gripping hand 79 of the first transfer part 71. In some embodiments, the first transferring member 71 may further include a first bracket 71f for mounting the first direction guide 71a described above.

In some embodiments, the gripping handle 79 of the first transferring member 71 grips, for example, a cuvette on the sample loading site 10a along the second direction, for example, the X direction in the drawing, so that the first transferring member 71 does not affect the sample dispensing member 30 to discharge the sample into the cuvette when gripping the cuvette, and thus the first transferring member 71 can make the sample dispensing member 30 complete the sample loading of the cuvette while gripping the cuvette, thereby saving time and improving the measurement speed and efficiency.

The above are some descriptions of the first transfer member 71.

The second transfer member 73 is used to transport the cuvette at the first buffer index 77 to the reaction member 51 and transport the cuvette at the reaction member 51 after incubation of the sample to the second buffer index 78. In some embodiments, the second transfer member 73 is configured to transfer the cuvette in the first buffer position 77 to the reaction member 51 by linear movement in a first direction, e.g., the direction in the figure, and a second direction, e.g., the direction X in the figure, and to transfer the cuvette in the reaction member 51 after incubation of the sample to the second buffer position 78. The second transfer component 73 moves along the straight line to move the reaction cup, so that the volume of the sample analysis device occupied in the process of conveying the reaction cup is relatively reduced, and the miniaturization design of the sample analysis device is facilitated.

In a specific transferring process, the second transferring component 73 may first transfer the reaction cup with the first buffer position 77 to the incubation position 51a, the reagent dispensing component 60 sucks the reagent and discharges the reagent into the reaction cup with the incubation position 51a, and the second transferring component 73 transfers the reaction cup with the incubation position 51a to the reaction component 51.

In some embodiments, the second transferring member 73 may move in a first direction (e.g., Y direction in the drawing), a second direction (e.g., X direction in the drawing), and a vertical direction (e.g., direction perpendicular to the drawing), so that the driving member of the second transferring member 73 may be a three-dimensional driving member for driving the cup gripping hand 79 of the second transferring member 73 to move in the first direction, the second direction, and the vertical direction. Referring to fig. 12 (c), in some embodiments, the second transferring member 73 includes a first direction guiding element 73a, a first direction driving member 73b, a second direction guiding element 73c, a second direction driving member 73d, a vertical direction guiding element 73e and a vertical direction driving member 73f, the first direction guiding element 73e is slidably provided with a cup gripping hand 79 of the second transferring member 73, and by driving of the vertical direction guiding element 73f, the cup gripping hand 79 can move in the vertical direction along the vertical direction guiding element 73e, the second direction guiding element 73c is slidably provided with the vertical direction guiding element 73e, and by driving of the second direction driving member 73d, the vertical direction guiding element 73e can move in the second direction along the second direction guiding element 73c, so that the cup gripping hand 79 of the second transferring member 73 is also driven in the second direction, the first direction guiding element 73c can move in the first direction along the first direction, and the first direction guiding element 73c can move in the first direction along the first direction, so that the cup gripping hand 79 can move in the first direction along the first direction guiding element 73c, and the cup gripping hand 79 can move in the first direction along the first direction guiding element 73 c. In particular embodiments, the first direction guide 73a may comprise a first rail, the first direction drive member 73b may comprise a first stepper motor, a first driven wheel, and a first timing belt, the first timing belt may be coupled between the first stepper motor and the first driven wheel, the second direction guide 73c may be fixedly coupled to the first timing belt, similarly, the second direction guide 73c may comprise a second rail, the second direction drive member 73d may comprise a second stepper motor, a second driven wheel, and a second timing belt, the second timing belt may be coupled between the second stepper motor and the second driven wheel, the vertical direction guide 73e may be fixedly coupled to the second timing belt, similarly, the vertical direction guide 73e may comprise a vertical rail, the vertical direction drive member 73f may comprise a lift stepper motor and a vertical screw, and a mounting plate may be threadably coupled to the vertical screw for mounting the grippers 79 of the second transfer member 73. In some embodiments, the second transfer member 73 may further include a second bracket 73g for mounting the first direction guide 73a described above.

In some embodiments, the gripping cup hand 79 of the second transferring member 73 grips the reaction cup along the second direction, for example, the X direction in the drawing, so that the second transferring member 71 does not affect the reagent dispensing member 60, for example, the first reagent dispensing member, to add reagent to the reaction cup when gripping the reaction cup, and thus the reagent dispensing member 60 can complete reagent adding of the reaction cup while the second transferring member 73 grips the reaction cup, thereby saving time and improving measurement speed and efficiency. In some embodiments, the direction in which the second transfer member 73 grips the cuvette is greater than 90 degrees from the direction in which the first set of reagent needles move linearly, so that there is less likelihood of a collision between the motion of the second transfer member 73 gripping the cuvette and the motion of the first set of reagent needles, both of which may be quite reasonably independent and parallel.

In some embodiments, the second transfer member 73 also mixes the sample in the cuvette during transport of the cuvette from the incubation site 51a to the reaction member 51. For example, after the second transferring member 73 transfers the cuvette after the sample addition from the first buffer memory 77 and places the cuvette in the incubation space 51a, the reagent dispensing member 60 adds a reagent, for example, a first reagent, to the cuvette in the incubation space 51a, and the second transferring member 73 picks up the cuvette after the reagent addition, mixes the cuvette, and transfers the cuvette to the reaction member 51, specifically, the second transferring member 73 can drive the cuvette gripping hand 79 to shake rapidly through the driving member 71b to mix the sample in the cuvette gripped by the cuvette gripping hand 79. In addition, the second transfer component 73 is used for uniformly mixing when grabbing the reaction cup for transferring, so that time is saved, and the reaction cup is not required to be specially scheduled to be uniformly mixed by the corresponding uniform mixing mechanism.

The above are some of the descriptions of the second transfer element 73. The second transfer member 73 can and enables transfer of the cuvette between the first buffer index 77, the incubation index 51a, the cuvette 51 and the second buffer index 78 by linear movement in the first and second directions.

The third transfer member 75 is used to transport the cuvette indexed 78 in the second buffer to the assay part 52. In some embodiments, the third transfer member 75 transfers cuvettes indexed 78 in the second buffer to the assay member 52 by linear movement in a first direction, e.g., the direction of the figure, and linear movement in a second direction, e.g., the direction of the figure, the direction of the X. The third transfer component 75 moves along the straight line to move the reaction cup, so that the volume of the sample analysis device occupied in the process of transporting the reaction cup is relatively reduced, and the miniaturized design of the sample analysis device is facilitated.

In a specific transfer process, the third transfer member 75 may first transfer the cuvette at the second buffer index 78 to the measurement index 52a, the reagent dispensing member 60 aspirates the reagent and discharges the reagent into the cuvette at the measurement index 52a, and the third transfer member 75 then transfers the cuvette at the measurement index 75a to the measurement member 52. In some embodiments, when the third transfer member 75 transfers the cuvette from the second cache index 78 to the assay index 52a, the third transfer member 75 may not place the cuvette in the assay index 52a, but still grasp the cuvette, in which case the reagent dispensing member 60 draws reagent and discharges it to the cuvette, such that the time for the cuvette to eventually enter the assay component 52 from the second cache index 78 is reduced, increasing the test speed.

In some embodiments, the third transferring member 75 may be movable in a first direction (e.g., Y direction in the drawing), a second direction (e.g., X direction in the drawing), and a vertical direction (e.g., direction perpendicular to the drawing), so that the driving member of the third transferring member 75 may be a three-dimensional driving member for driving the cup gripping hand 79 of the third transferring member 75 to move in the first direction, the second direction, and the vertical direction. Referring to fig. 12 (d), in some embodiments, the third transferring member 75 includes a first direction guiding element 75a, a first direction driving member 75b, a second direction guiding element 75c, a second direction driving member 75d, a vertical direction guiding element 75e and a vertical direction driving member 75f, the first direction guiding element 75e is slidably provided with a cup gripping hand 79 of the third transferring member 75, and by driving of the vertical direction guiding element 75f, the cup gripping hand 79 can move in the vertical direction along the vertical direction guiding element 75e, the second direction guiding element 75c is slidably provided with the vertical direction guiding element 75e, and by driving of the second direction driving member 75d, the vertical direction guiding element 75e can move in the second direction along the second direction guiding element 75c, so that the cup gripping hand 79 of the third transferring member 75 is also driven to move in the second direction, the first direction guiding element 75c is also driven to move in the first direction along the first direction guiding element 75c, and the third direction guiding element 75c is driven to move in the vertical direction along the first direction guiding element 75c, so that the cup gripping hand 79 can move in the first direction along the first direction guiding element 75c is driven by driving of the first direction driving member 75 c. In particular embodiments, the first direction guide 75a may comprise a first rail, the first direction drive member 75b may comprise a first stepper motor, a first driven wheel, and a first timing belt, the first timing belt may be coupled between the first stepper motor and the first driven wheel, the second direction guide 75c may be fixedly coupled to the first timing belt, similarly, the second direction guide 75c may comprise a second rail, the second direction drive member 75d may comprise a second stepper motor, a second driven wheel, and a second timing belt, the second timing belt may be coupled between the second stepper motor and the second driven wheel, the vertical direction guide 75e may be fixedly coupled to the second timing belt, similarly, the vertical direction guide 75e may comprise a vertical rail, the vertical direction drive member 75f may comprise a lift stepper motor and a vertical screw, and a mounting plate may be threadably coupled to the vertical screw for mounting the grippers 79 of the third transfer member 75. In some embodiments, the third transfer member 75 may further include a second bracket 75g for mounting the first direction guide 75a described above.

In some embodiments, the gripping handle 79 of the third transfer member 75 grips the cuvette in a first direction, such as the Y direction in the drawing, so that the third transfer member 75 grips the cuvette and, even if the third transfer member 75 grips the cuvette during the whole reagent adding process, does not affect the reagent adding process of the reagent dispensing member 60, such as the second reagent dispensing member, to the cuvette, and thus the reagent dispensing member 60 can complete reagent adding of the cuvette while the third transfer member 75 grips the cuvette, thereby saving time and improving the measuring speed and efficiency. In some embodiments, the direction in which the third transfer member 75 grips the cuvette is greater than 90 degrees from the direction in which the second set of reagent needles is linearly moved, so that there is less likelihood of a collision between the motion of the third transfer member 75 gripping the cuvette and the motion of the second set of reagent needles, both of which may be quite reasonably independent and parallel.

In some embodiments, the third transfer member 75 mixes the sample in the cuvette during transport of the cuvette from the index 52a to the measurement unit 52. For example, when the third transferring member 75 transfers the cuvette from the second buffer memory 78 to the in-assay transfer 52a, in some embodiments, the third transferring member 75 may not put down the cuvette and still grasp the cuvette when transferring the cuvette to the in-assay transfer 52a, the reagent dispensing member 60 may mix the sample in the grasped cuvette after adding a reagent such as the second reagent to the cuvette in the in-assay transfer 52a, and then the third transferring member 75 may transfer the sample to the measuring member 52, and in particular, the third transferring member 75 may drive the gripper 79 to shake rapidly by the driving member 71b to mix the sample in the cuvette grasped by the gripper 79. In addition, the third transfer component 75 is used for uniformly mixing the reaction cups on the original transfer path, such as the measurement center position 52a, so that the time is saved, and the reaction cups do not need to be specially scheduled to the corresponding uniform mixing mechanism for uniform mixing.

In some examples, the third transferring member 75 may further grasp the cuvette that has been measured in the measuring member 52 after the cuvette is transferred to the measuring member 52, and then transfer the cuvette to the second cuvette position 10d for cuvette polishing, and in some examples, the second cuvette position 10d may be disposed near the second buffer memory location 78 or between the measuring member 52 and the second buffer memory location 78, so that the third transferring member 75 may perform the cuvette polishing for the cuvette that has been measured on the measuring member 52 when transferring the cuvette from the measuring member 52 to the second buffer memory location 78 to transfer the cuvette on the second buffer memory location 78, thereby saving time and improving the testing efficiency.

Some of the descriptions above are for the third transfer element 75. The third transfer member 75, by linear movement in the first and second directions, can and effect transfer of the cuvette between the second buffer indexing 78, the assay indexing 52a, the assay member 52 and even the second cuvette position 10 d.

The foregoing is illustrative of the dispatch unit 70 of some embodiments of the present application, wherein the present application provides for the rapid transfer of the cuvette by three transfer units, namely, a first transfer unit 71, a second transfer unit 73, and a third transfer unit 75, wherein the cuvette is simply and directly dispatched, which facilitates the speed up of the sample analysis device, and wherein the transition between the three transfer units is also structurally simple and compact by combining two in-buffer-index units, namely, a first in-buffer-index unit 77 and a second in-buffer-index unit 78.

The foregoing is a sample analysis device disclosed in some embodiments of the invention. It will be appreciated that the disclosed sample analysis device may also include some other structure, such as a cleaning member 80 and/or a processor 90, etc., as will be specifically described below in connection with fig. 15 and 16.

The cleaning member 80 is used to clean the reagent needles, for example, to clean the first reagent needle and the second reagent needle, etc. Specifically, the washing part 80 may include a plurality of washing wells 81, and the number of washing wells 81 may be the same as the number of reagent needles, for example, when the sample analysis device includes a first reagent dispensing part and a second reagent dispensing part, each of which includes two reagent needles, the number of washing wells may be four. A cleaning tank can be arranged on the linear motion track of each reagent needle for cleaning the reagent needles. Referring to fig. 16, the cleaning member 80 is used for cleaning the reagent needle, specifically, cleaning the inner wall and the outer wall of the reagent needle by a cleaning solution. The cleaning member 80 includes a cleaning tank, a pipe, an on-off valve provided on the pipe, and the like in the drawing. The end of the reagent needle can be connected with a pipeline which is opened and closed by the switch valve SV01, when the switch valve SV01 is opened, the cleaning liquid can reach the end of the reagent needle through the pipeline, flow through the inner wall of the reagent needle and flow out from the front end of the reagent needle, and the cleaning of the cleaning liquid on the inner wall of the reagent needle is completed. The cleaning chamber is also connected with a pipeline which is opened and closed by the switch valve SV02, and when the switch valve SV02 is opened, cleaning liquid can reach the cleaning chamber through the pipeline and be sprayed out from the inner wall of the cleaning chamber to the outer wall of the reagent needle, so that the cleaning liquid can clean the outer wall of the reagent needle. The lower end of the cleaning chamber is connected with a waste liquid suction valve SV03 through a pipeline, and when the waste liquid suction valve SV03 is opened, the cleaned waste liquid flows out through the lower end of the cleaning chamber. During specific cleaning, the reagent needle comes to the upper part of the cleaning chamber, and then moves downwards to extend the part of the reagent needle (at least comprising the part of the needle body which is contacted with the liquid level of the reagent during reagent sucking) into the cleaning chamber, so that the cleaning liquid sprayed in the cleaning chamber can clean the part of the needle body which is contacted with the liquid level of the reagent needle, and the cleaning of the reagent needle is completed. The washing tanks 81 may share the same set of liquid paths to provide washing liquid for washing the reagent needles.

Some specific working flows of the sample analysis device are described below.

The sample analysis device in some embodiments may be operated as such.

The cuvette loading section 10 supplies and carries empty cuvettes. For example, the cuvette loading section 10 may load an empty cuvette to a predetermined position, which may be used as a loading site. The sample section 20, e.g. the sample section 21, dispatches a sample rack carrying samples to the sample suction position. The sample dispensing part 30 sucks a sample from the sample sucking position and dispenses it into a reaction cup, for example, the sample dispensing part sucks a sample from the sample sucking position and discharges it to the reaction cup located on the sample adding position to complete sample adding.

The drive member of the reagent carrier member 60 drives the reagent carrier member 60 in rotation such that the reagent container carrying the first reagent is located in the first reagent sucking position. At least one of the two reagent needles 61 on the first reagent dispensing part 60 sucks the first reagent in the reagent vessel through the first reagent sucking site and moves linearly between the first reagent sucking site and the reagent transferring site of the reaction part 51 to dispense the first reagent to the reagent cup of the reaction part 51 where the reagent transferring site is located. Reagent-added translocation of reaction component 51 may be the incubation translocation 51a referred to herein. In some embodiments, the two first reagent needles 61 of the first reagent dispensing component 60 move linearly independently of each other between the first reagent aspirating position and the indexing in the reagent of the reaction component 51. Thus, the two first reagent needles 61 of the first reagent dispensing part 60 can perform the operation of adding the first reagent to the reagent cups of the reagent adding and indexing part of the reaction part 51 independently, for example, alternately, thereby improving the test speed and efficiency. In some embodiments, each of the first reagent needles 61 in the first reagent dispensing part 60 sequentially performs a plurality of preset actions to complete the first reagent adding operation, and at least one corresponding preset action among the plurality of preset actions between every two first reagent needles 61 is not overlapped in time sequence. In this way, the two reagent needles 61 of the first reagent dispensing part 60 can avoid occupying as little common resources as possible, so that the number of parts providing corresponding common resources can be reduced, and the sample analysis device can be more compact, and the timing of the operations of the two first reagent needles 61 can be arranged so as to avoid the mutual influence therebetween as much as possible, which is advantageous for the acceleration of the sample analysis device.

The dispensing unit 70 dispenses the cuvette for which the first reagent dispensing is completed to the reaction unit 51 for incubation, and dispenses the cuvette for which incubation is completed to the reagent adding unit 52 for indexing. The reagent-added index of the assay component 52 may be the assay index 52a referred to herein.

The reagent carrier 40 rotates such that the reagent container carrying the second reagent is located in the second reagent sucking position. At least one of the two reagent needles on the second reagent dispensing part 60 sucks the second reagent in the reagent vessel through the second reagent sucking site and moves linearly between the second reagent sucking site and the reagent adding position of the measuring part to dispense the second reagent to the reagent cup of the measuring part 52 where the reagent adding position is shifted. In some embodiments, the two second reagent needles 61 of the second reagent dispensing component 60 move linearly independently of each other between the second reagent aspirating position and the indexing in the reagent of the assay component 52. Thus, the two second reagent needles 61 of the second reagent dispensing part 60 can perform the operation of adding the second reagent to the reagent cups of the measuring part 52, which are indexed with respect to the reagent, respectively and independently, for example, alternately, thereby improving the test speed and efficiency. In some specific embodiments, each of the second reagent needles 61 in the second reagent dispensing part 60 sequentially performs a plurality of preset actions to complete the second reagent adding operation, and at least one corresponding preset action among the plurality of preset actions between every two second reagent needles 61 is not overlapped in time sequence. In this way, the two reagent needles 61 of the second reagent dispensing part 60 can avoid occupying as little common resources as possible, so that the number of parts providing corresponding common resources can be reduced, and the sample analysis device can be more compact, and the timing of the actions of the two second reagent needles can be arranged so as to avoid the mutual influence therebetween as much as possible, which is advantageous for the acceleration of the sample analysis device.

The dispatch component 70 dispatches the cuvette for which the second reagent dispensing is completed to the measurement component 52 for item testing, and dispatches the cuvette after the testing is completed to a discard recycling apparatus, such as the second cuvette position mentioned herein.

A sample analysis method is also disclosed in some embodiments of the invention. Referring to fig. 17, in some embodiments, the sample analysis method includes the following steps:

Step 100, the cuvette loading step, controls the cuvette loading section to supply and carry an empty cuvette. For example, the cuvette loading section may load an empty cuvette to a predetermined position, which may be used as a loading site.

Step 110, i.e. the sample feeding step, controls the sample component, e.g. the sample feeding component, to dispatch the sample rack carrying the samples to the sample suction position.

Step 120, namely a sample dispensing step, the sample dispensing component is controlled to suck the sample from the sample sucking position and dispense the sample into the reaction cup. For example, the sample dispensing part sucks and samples from the sample sucking position and discharges the sample to a reaction cup positioned on the sample adding position so as to complete sample adding.

The loading is completed through the above steps 100 to 120.

And 130, namely a first reagent dispensing step, controlling a driving part of the reagent bearing part to drive the reagent bearing part to rotate so that the reagent container bearing the first reagent is positioned at a first reagent sucking position, and controlling at least one of two reagent needles on the first reagent dispensing part to suck the first reagent in the reagent container through the first reagent sucking position and make linear motion between the first reagent sucking position and the transposition in the reagent adding of the reaction part so as to dispense the first reagent into the reaction cup with the transposition in the reagent adding of the reaction part. The indexing of the reagent addition of the reaction component in step 130 may be the indexing of the incubation referred to herein.

In some embodiments, step 130 controls the two first reagent needles of the first reagent dispensing component to move linearly independently of each other between the first reagent aspirating position and the indexing in the reagent of the reaction component. Thus, the two first reagent needles of the first reagent dispensing component can independently and respectively, for example, alternately complete the operation of adding the first reagent to the reaction cup which is shifted in the reagent adding process of the reaction component, thereby improving the testing speed and the testing efficiency. In some specific embodiments, step 130 controls each of the first reagent needles in the first reagent dispensing component to perform a plurality of preset actions in sequence to complete the first reagent adding operation, and at least one corresponding preset action among the plurality of preset actions between every two first reagent needles is not overlapped in time sequence. In this way, the two reagent needles of the first reagent dispensing part can avoid occupying less common resources as much as possible, so that the number of parts providing corresponding common resources can be reduced, and the sample analysis device can be more compact, and the action time sequences of the two first reagent needles are arranged, so that the mutual influence between the two first reagent needles is avoided as much as possible, which is very beneficial to the speed up of the sample analysis device. It should be noted that the plurality of preset actions may be different according to different test requirements, and in one embodiment, the plurality of preset actions includes 4 actions of sucking reagent, heating, discharging reagent and cleaning. At least one of the 4 actions is non-overlapping in time sequence between the two first reagent needles.

The addition of the first reagent to the cuvette with the sample is completed by step 130.

Step 140, namely an incubation step, the control dispatching component dispatches the reaction cup with the first reagent dispensed to the reaction component for incubation, and dispatches the reaction cup after incubation to the reagent adding component for transposition. In step 140, the reagent-added indexing of the assay component may be the assay indexing referred to herein.

And 150, namely a second reagent dispensing step, controlling the reagent bearing component to rotate so that the reagent container bearing the second reagent is positioned at a second reagent sucking position, and controlling at least one of the two reagent needles on the second reagent dispensing component to suck the second reagent in the reagent container through the second reagent sucking position and make linear motion between the second reagent sucking position and the indexing in the reagent adding of the measuring component so as to dispense the second reagent into the reaction cup of the indexing in the reagent adding of the measuring component.

In some embodiments, step 150 controls the linear movement of the two second reagent needles of the second reagent dispensing component independently of each other between the second reagent aspirating position and the indexing in the reagent of the assay component. Thus, the two second reagent needles of the second reagent dispensing part can independently and respectively, for example, alternately complete the operation of adding the second reagent to the reaction cup which is shifted in the reagent adding process of the measuring part, thereby improving the testing speed and the testing efficiency. In some specific embodiments, step 150 controls each of the second reagent needles in the second reagent dispensing component to perform a plurality of preset actions in sequence to complete the second reagent adding operation, and at least one corresponding preset action among the plurality of preset actions between every two second reagent needles is not overlapped in time sequence. In this way, the two reagent needles of the second reagent dispensing part can avoid occupying less common resources as much as possible, so that the number of parts providing corresponding common resources can be reduced, and the sample analysis device can be more compact, and the action time sequences of the two second reagent needles are arranged, so that the mutual influence between the two second reagent needles is avoided as much as possible, which is very beneficial to the speed up of the sample analysis device. It should be noted that the plurality of preset actions may be different according to different test requirements, and in one embodiment, the plurality of preset actions includes 4 actions of sucking reagent, heating, discharging reagent and cleaning. At least one of the 4 actions is non-overlapping in time sequence between every two second reagent needles.

Continued addition of the second reagent to the cuvette carrying the incubated reagent is completed by step 150.

Step 160, the measuring and recycling step, controls the dispatching means to dispatch the cuvette for which the second reagent dispensing is completed to the measuring means for item detection and to dispatch the cuvette after detection to a discard recycling apparatus, which may have, for example, a second cuvette throwing position as referred to herein.

The above is an overall flow of sample analysis device operation.

The workflow arrangement of the reagent dispensing component 60 places important constraints on the speed of detection, and each reagent needle is required to perform the actions of sample suction, heating, sample discharge, and cleaning. The reagent carrying member 40 is used for securing the activity of the reagent, and is typically at a low temperature, for example, 16 ℃ or less. After the reagent is taken out from the reagent carrying member 40, heating to about 37 ℃ is completed in the reagent needle in a short time to ensure that the reaction process is sufficient. In general, the heating means of the reagent needle needs 4 to 10 seconds for heating the reagent sucked by the reagent needle. In the process of detecting different detection items, the reagent needle needs to suck different types of reagents, such as the conventional four items of coagulation (PT/APTT/TT/FIB) item detection, and needs to suck different second reagents, namely triggering reagents, from the reagent bearing component 40 in turn to be added into the reaction cup for reaction. Therefore, the same reagent needle needs to be subjected to normal washing or strong washing when the reagent of different projects is sucked. In order to ensure the cleaning effect and avoid the influence of the carrying pollution among the reagents on the accuracy of the detection result, the cleaning time generally needs 2-8 seconds. The sucking and discharging operations generally require 1.5-3 s (including horizontal movement in place). Therefore, for example, with a sample analyzer having a single operation cycle of 8 seconds, it is difficult to complete all of the operations of sucking, heating, discharging, and cleaning in one operation cycle, and the overall detection speed is reduced.

In some embodiments of the present invention, how to perform reagent adding operation, i.e., how to schedule the operation of each reagent needle, is designed, and is described in detail below.

In some embodiments, the processor 90 is configured to control each reagent needle in the same group to sequentially perform a plurality of preset actions, such as a reagent sucking action, a reagent heating action in the reagent needle, a reagent discharging action, and a reagent needle cleaning action, so as to complete the reagent adding operation, and at least one preset action corresponding to the plurality of preset actions between every two reagent needles in the same group is not overlapped in time sequence. For example, the first set of reagent needles in the first reagent dispensing component 60 comprises two first reagent needles, at least one corresponding preset motion between the two first reagent needles does not overlap in time sequence, and for example, the second set of reagent needles in the second reagent dispensing component 60 comprises two second reagent needles, at least one corresponding preset motion between the two second reagent needles does not overlap in time sequence. In some embodiments, a ping-pong mode may be provided for the sample analysis device, which when enabled, is executed by the processor 90 such that at least one corresponding action between every two reagent needles in each set of reagent needles does not overlap in time sequence, e.g., at least one corresponding action in a reagent needle of the same set of reagent needles, a reagent heating action in a reagent needle, a reagent discharging action, and a reagent needle washing action does not overlap in time sequence, the at least one corresponding action including a reagent sucking action and/or a reagent needle washing action. In some other embodiments, the processor controls each reagent to sequentially perform a plurality of preset actions to complete the reagent adding operation, and at least one corresponding preset action among the plurality of preset actions between every two reagent needles is not overlapped in time sequence. The first reagent needle includes a first reagent needle a1, a first reagent needle a2, and the second reagent needle includes a second reagent needle b1 and a second reagent needle b2. The processor controls at least one corresponding preset action not to overlap in time sequence when the first reagent needle a1, the first reagent needle a2, the second reagent needle b1 and the second reagent needle b2 each complete a plurality of preset actions. All reagent needles in the sample analysis device are subjected to time sequence arrangement through the processor, so that the mutual interference of actions among the reagent needles is avoided, resources are preempted, the common resources are more effectively utilized, and the sample testing efficiency of the sample analysis device is improved.

In some embodiments, the plurality of preset actions may include a first type of preset action and a second type of preset action. The first type of preset actions are actions that each reagent needle needs to interact with the same component, and in general, the first type of preset actions may be actions that the reagent needle needs to occupy a common resource, such as a reagent sucking action that needs to occupy a common component of the reagent carrying component 40, such as a reagent needle cleaning action that needs to occupy a pipeline that commonly provides cleaning liquid to each cleaning tank 81, and thus, the first type of preset actions at least includes a reagent sucking action and a reagent needle cleaning action. The second type of preset actions are actions in which each reagent does not need to interact with the same component, and in general, the second type of preset actions may be actions in which the reagent needles do not need to occupy common resources, such as reagent heating actions in which each reagent needle heats the sucked reagent through a respective heating component, and thus the second type of preset actions at least includes reagent heating actions in the reagent needles. In some embodiments, the first type of preset motion corresponding to the two reagent needles in the same group does not overlap in time sequence, for example, the reagent sucking motion between the two reagent needles in the same group does not overlap in time sequence, and the reagent needle cleaning motion does not overlap in time sequence, for example, as shown in fig. 18.

In some embodiments, each of the plurality of preset actions between every two reagent needles in the same group does not overlap in time sequence. For example, fig. 19 is an example. The first set of reagent needles in the first reagent dispensing component 60 includes two first reagent needles, the reagent sucking action between the two first reagent needles does not overlap in time sequence, the reagent heating action in the reagent needles does not overlap in time sequence, the reagent discharging action in the reagent needles does not overlap in time sequence, the reagent sucking action in the reagent needle cleaning action does not overlap in time sequence, and for example, the reagent sucking action between the two second reagent needles in the second reagent dispensing component 60 includes two second reagent needles, the reagent sucking action between the two second reagent needles does not overlap in time sequence, the reagent heating action in the reagent needles does not overlap in time sequence, the reagent discharging action in the reagent needles does not overlap in time sequence, and the reagent sucking action in the reagent needle cleaning action does not overlap in time sequence.

In some embodiments, the time interval between outputting results of two adjacent and identical test items when the sample analyzer completes a fixed test amount is defined as one cycle, and the number of reagent needles of each reagent dispensing component is equal to the number of cycles occupied by one reagent needle to complete the plurality of preset actions. For example, if the number of cycles taken by one reagent needle to perform the above-described predetermined operations (for example, the reagent sucking operation, the reagent heating operation in the reagent needle, the reagent discharging operation, and the reagent needle cleaning operation) is two, two reagent needles are provided to the reagent dispensing part, or the number of reagent needles in the same group is two. From another perspective, in some embodiments, processor 90 controls each reagent needle of the reagent dispensing component to complete the plurality of preset actions (e.g., the reagent sucking action, the reagent heating action in the reagent needle, the reagent discharging action, and the reagent needle cleaning action) within a preset time period equal to N times the cycle, N being equal to the number of reagent needles of the reagent dispensing component. For example, the number of reagent needles of each reagent dispensing part 60 is two, then N is equal to two, and each reagent needle needs to complete the plurality of preset actions (e.g., the reagent sucking action, the reagent heating action in the reagent needle, the reagent discharging action, and the reagent needle cleaning action) in two cycles. By setting the number of reagent needles of the reagent dispensing part and the cycles for completing the plurality of preset actions, the sample analysis device can be equivalent to the fact that each reagent needle completes the plurality of preset actions (such as a reagent sucking action, a reagent heating action in the reagent needle, a reagent discharging action and a reagent needle cleaning action) in one cycle in the test speed, and the speed of outputting the detection result of the sample analysis device is ensured to be constant. here, the preset time is mentioned, and in some embodiments, when the time spent by the reagent needle to perform the plurality of preset actions is less than the preset time, in order to further ensure that the speed of outputting the detection result by the sample analysis device is constant, the reagent needle waits such that the sum of the waiting time and the time spent by the plurality of preset actions is equal to the preset time. In order to be more clearly described, the preset time includes an action time for performing the above-described preset actions (e.g., a reagent sucking action, a reagent heating action in the reagent needle, a reagent discharging action, and a reagent needle washing action) and a waiting time, and the action time is less than or equal to the preset time. In some embodiments, the wait time is divided into one or more periods of time and is inserted in time between the plurality of preset actions and/or after the last preset action, such as in time between the pipetting action, the reagent heating action in the reagent needle, the reagent discharging action and the reagent needle washing action and/or after the reagent needle washing action, such as in FIG. 20, for example. In some embodiments, the wait time is divided into one or more periods of time and at least one period of time is used as an additional action time in time sequence for performing the preset action. in this way, the preset action can be continued to be executed for additional time, under the constraint of the preset time, the execution time of the preset action is prolonged, the reagent needle can be enabled to have more stable performance, for example, the execution time of the reagent sucking action and the reagent discharging action is prolonged, the reagent needle can be enabled to stably suck the reagent and the reagent discharging action, the condition of air suction or collision with a reaction cup and the like is not easy to be caused, for example, the execution time of the reagent heating action in the reagent needle is prolonged, the reagent in the reagent is fully preheated, for example, the execution time of the reagent needle cleaning action is prolonged, the reagent needle is cleaned more fully, cross contamination to the next test item is not easy to be caused, and the accuracy of the test result is improved. Thus, in some embodiments, the sample analysis device may include a full heating mode that, when enabled, is executed by the processor 90 such that the wait time is divided into one or more segments and at least one segment is used as an additional action time in time sequence for performing a reagent heating action in the reagent needle. For example, fig. 21 is an example.

It should be noted that, in fig. 18 to 21, for convenience of drawing, the heating action refers to the reagent heating action in the reagent needle herein, the washing action refers to the reagent needle washing action herein, and the two reagent needles shown in fig. 18 to 21 refer to two reagent needles in the same group, for example, two first reagent needles in the first group of reagent needles or two second reagent needles in the second group of reagent needles.

According to the invention, the action time sequence of the reagent needle is designed, the reagent dispensing component is introduced to be provided with two reagent needles which are arranged in parallel, the independent and straight-line running double reagent needles are used for completing the whole working procedures of reagent sucking, preheating, reagent discharging and cleaning through ping-pong scheduling, double resources are provided, the speed is ensured to be improved, and the aim that the test of a plurality of detection items is not slowed down is fulfilled.

The following describes how the cuvette is dispatched by the dispatch unit 70. In some embodiments, the invention provides for transfer of the cuvette by indexing in three transfer members and two buffers.

In some embodiments, referring to fig. 22, a method of a sample analysis device is disclosed, which may include the following steps:

Step 200, controlling a first transferring component to transfer the reaction cup with the sample being added to a first buffer for transferring. In some embodiments, step 200 controls the first transfer member to move linearly in a first direction to transfer the loaded cuvette to the first buffer for indexing.

Step 210, controlling the second transfer component to transport the cuvette indexed in the first buffer to the incubation site. The incubation site here may be a cuvette placement site in the reaction component 51. In some embodiments, step 210 controls the second transfer member to transport the cuvette indexed in the first buffer to the incubation site by linear motion in the first direction and linear motion in the second direction.

Step 220, controlling the second transferring component to transfer the reaction cup with the sample in the incubation position to the second buffer for indexing.

In some embodiments, step 220 controls the second transfer member to transfer the cuvette for which incubation of the sample in the incubation site is completed to the second buffer for indexing by linear movement in the first direction and linear movement in the second direction. In some embodiments, step 220 controls the second transfer component to first transfer the cuvette indexed in the first buffer to the incubation for reagent addition, and then transfer the cuvette indexed during the incubation to the incubation site.

In some embodiments, step 220 controls the second transfer member to mix the sample in the cuvette during transfer of the cuvette from the incubation site to the incubation site. When the second transfer component transfers the reaction cup, the second transfer component is controlled to uniformly mix the samples in the reaction cup, so that the time is saved, and the reaction cup does not need to be specially scheduled to a corresponding uniform mixing mechanism for uniform mixing.

Step 230, controlling the third transfer component to transfer the indexed cuvette in the second buffer to the measurement site.

In some embodiments, step 230 controls the third transfer member to transport the indexed cuvette in the second buffer to the testing site by linear motion in the first direction and linear motion in the second direction. In particular embodiments, step 230 controls the second transfer component to first transfer the cuvette indexed in the second buffer to the assay for reagent addition, and then transfer the cuvette indexed in the assay with reagent addition to the assay site.

In some specific embodiments, step 230 controls the third transferring component to mix the sample in the cuvette during transferring the cuvette from the second buffer memory to the measuring site. When the third transfer component transfers the reaction cup, the third transfer component is controlled to uniformly mix the sample in the reaction cup, so that the time is saved, and the reaction cup is not required to be specially scheduled to a corresponding uniform mixing mechanism for uniform mixing.

The three transfer components move along the straight line to realize the rapid transfer of the reaction cup, and the transfer of the reaction cup is matched with the transfer of the two caches, so that the dispatching path of the reaction cup is simple and direct, and the acceleration of the sample analysis device is facilitated.

Finally, the present invention will be described with reference to a specific test item by taking as an example a sample analyzer including a first reagent dispensing member 60 having two first reagent pins 61, a second reagent dispensing member 60 having two second reagent pins 61, a reaction member 51, and a measurement member 52.

The reaction block 51 has a number of cuvette placement sites and can heat the sample in the cuvette placed on the cuvette placement sites to incubate the sample. Depending on the test item, some test items may require the addition of a first reagent, such as a mixed reagent. For example, when performing a test on the measurement item APTT based on the coagulation method, the first reagent dispensing part 60 sucks the first reagent, for example, a mixed reagent, from the reagent carrying part 40 and discharges the sucked first reagent into a reaction cup located at the incubation center 51a of the reaction part 51, thereby completing the mixing of the first reagent and the sample. After the addition of the mixed reagent is completed, the second transfer member 73 may mix the reaction solution in the cuvette, and then place the cuvette in the reaction member 51, and the reaction member 51 incubates the reaction solution or the sample in the cuvette.

The reagent carrier 40 is designed to ensure the activity of the reagent and therefore typically operates at a relatively low temperature, for example typically below 16 ℃. To ensure that the coagulation reaction process is sufficient to obtain accurate test results, the first reagent needs to be heated to about 37 ℃ before being added to the reaction cup to mix with the sample. In order to increase the test speed of the sample analyzer, it is necessary to complete the heating of the first reagent in a short time, and therefore, both the first reagent needles 61 of the first reagent dispensing part 60 have heating parts for completing the reagent heating function. In general, the heating time of the reagent needs 4 to 10 seconds, and for a high-speed sample analysis device, the single working cycle is 8 seconds, for example, so that the heating time of the reagent takes longer, which greatly affects the test speed of the sample analysis device. Thus, in some embodiments of the present invention, the first reagent dispensing part 60 has two first reagent needles 61, and the two first reagent needles 61 respectively draw the first reagent, such as a mixed reagent, from the reagent carrying part 40, move to the incubation index 51a through the linear guide fixed to the linear beam, and alternately add the first reagent, such as a mixed reagent, to the reaction cup. The two first reagent needles 61 are arranged in parallel and move independently. By having two separate first reagent needles 61, the duty cycle time of the first reagent dispensing member 60 is doubled, which can be extended to 16 seconds, and the heating member of the reagent needles can be sufficiently ensured to have a sufficient heating time for the first reagent, so that the reagent temperature is stabilized to 37 ℃.

After the sample in the cuvette has been heated in the reaction part 51 for a fixed period of time for incubation, the cuvette is transported towards the measurement part 52 by cooperation of the second transport part 73 and the third transport part 75, in some embodiments the cuvette may be passed through the assay site 52a for addition of a second reagent, e.g. a trigger reagent.

The third transfer member 75 transfers the cuvette to the measurement center 52a, and the second reagent dispensing member 60 sucks a second reagent, such as a trigger reagent, from the reagent carrying member 40, moves over the cuvette gripped by the third transfer member 75, and adds the second reagent, such as the trigger reagent, to the cuvette, thereby completing mixing of the second reagent and the sample. After the addition of the trigger reagent is completed, the third transfer member 75 may mix the reaction solution in the cuvette, and then place the cuvette in the measurement member 52 to perform coagulation signal analysis and detection, thereby obtaining a detection result.

Similar to the first reagent dispensing part 60, to ensure that the second reagent, e.g. the trigger reagent, has a sufficient heating time, the second reagent dispensing part 60 likewise has two reagent needles, e.g. two second reagent needles, which each suck the second reagent, e.g. the trigger reagent, from the reagent carrier part 40, which are moved to the measuring index 52a by means of a linear guide fixed to a linear beam, and which in turn add the second reagent, e.g. the trigger reagent, to the reaction cup. The two second reagent needles 61 are arranged in parallel and move independently. By having two separate second reagent needles 61, the duty cycle time of the second reagent dispensing member 60 is doubled, and can be extended to 16 seconds, and the heating member of the reagent needles can be sufficiently ensured to have a sufficient heating time for the second reagent, so that the reagent temperature is stabilized to 37 ℃.

The cuvette in the measuring section 52 is irradiated with light of a plurality of wavelengths, receives its transmitted light or scattered light with, for example, a photodetector in the measuring section 52, and outputs a detection signal corresponding to the amount of the received light, which can be sent to, for example, the processor 90 for analysis of data, processing, and generation of corresponding display contents. In a sample analyzer, for example, a full-automatic coagulation analyzer, sample analysis may be performed by using different methods such as a coagulation method, an immunoturbidimetry method, and a chromogenic substrate method, and light having different wavelengths may be irradiated to a cuvette in the measuring unit 52, for example, in a wavelength range of 405nm to 800nm, depending on the detection method.

The cuvette for which the test is completed may be transferred by a third transfer member 75 to a discard recovery device, which may have, for example, a second cuvette position as referred to herein, into which the third transfer member 75 discards the cuvette, completing the discard process of the cuvette.

Reference is made to various exemplary embodiments herein. However, those skilled in the art will recognize that changes and modifications may be made to the exemplary embodiments without departing from the scope herein. For example, the various operational steps and components used to perform the operational steps may be implemented in different ways (e.g., one or more steps may be deleted, modified, or combined into other steps) depending on the particular application or taking into account any number of cost functions associated with the operation of the system.

In the above embodiments, it may be implemented in whole or in part by software, hardware, firmware, or any combination thereof. Additionally, as will be appreciated by one of skill in the art, the principles herein may be reflected in a computer program product on a computer readable storage medium preloaded with computer readable program code. Any tangible, non-transitory computer readable storage medium may be used, including magnetic storage devices (hard disks, floppy disks, etc.), optical storage devices (CD-to-ROM, DVD, blu-Ray disks, etc.), flash memory, and/or the like. These computer program instructions may be loaded onto a general purpose computer, special purpose computer, or other programmable data processing apparatus to produce a machine, such that the instructions which execute on the computer or other programmable data processing apparatus create means for implementing the functions specified. These computer program instructions may also be stored in a computer-readable memory that can direct a computer or other programmable data processing apparatus to function in a particular manner, such that the instructions stored in the computer-readable memory produce an article of manufacture including means which implement the function specified. The computer program instructions may also be loaded onto a computer or other programmable data processing apparatus to cause a series of operational steps to be performed on the computer or other programmable apparatus to produce a computer implemented process such that the instructions which execute on the computer or other programmable apparatus provide steps for implementing the functions specified.

Claims

1. A sample analysis device, comprising:

chassis;

A reaction cup loading component, used for supplying and carrying empty reaction cups;

The sample introduction component is used to dispatch the sample rack carrying the sample to the sample suction position;

The sample dispensing component is used to absorb the sample from the sample suction position and discharge it into the reaction cup located at the sample adding position;

A reagent carrying component, the reagent carrying component is arranged in a disc-shaped structure, and has a plurality of positions for a first reagent container for carrying a first reagent, and a plurality of positions for a second reagent container for carrying a second reagent; the reagent carrying component includes a first driving component for driving the rotation thereof, the first driving component drives the reagent carrying component to rotate and drives the first reagent container to rotate, so as to rotate the first reagent container to a first reagent suction position; the first driving component drives the reagent carrying component to rotate and drives the second reagent container to rotate, so as to rotate the second reagent container to a second reagent suction position;

A reaction component, used for carrying a reaction cup and incubating the sample in the reaction cup, wherein the reaction component is provided with at least one incubation transfer position for placing the reaction cup;

A measuring component, used for carrying a reaction cup and detecting a sample in the reaction cup, wherein the measuring component is provided with at least one measuring transfer position for placing the reaction cup;

The first reagent dispensing component includes: a first crossbeam and a first group of reagent needles, wherein the first crossbeam is provided as one, and the first group of reagent needles includes two first reagent needles; the two first reagent needles are provided on the first crossbeam and move linearly along the long axis direction of the first crossbeam to absorb the first reagent from the first reagent aspiration position and discharge it into the reaction cup located at the incubation transfer position;

The second reagent dispensing component includes: a second crossbeam and a second group of reagent needles, the second crossbeam is provided as one, and the second group of reagent needles includes two second reagent needles; the two second reagent needles are provided on the second crossbeam and move linearly along the long axis direction of the second crossbeam to absorb the second reagent from the second reagent aspiration position and discharge it into the reaction cup located at the measurement transfer position;

The scheduling component is used to schedule the reaction cups; the scheduling component schedules the reaction cup with the first reagent added at the incubation transfer position to the reaction cup placement position of the reaction component, and schedules the reaction cup with the second reagent added at the measurement transfer position to the reaction cup placement position of the measurement component.

2. The sample analysis device according to claim 1, wherein:

The first crossbeam is arranged along the direction determined by the first reagent aspiration position and the incubation transfer position, and the first crossbeam is fixedly arranged at an upper position corresponding to the first reagent aspiration position and the incubation transfer position;

The second crossbeam is arranged along the direction determined by the second reagent aspiration position and the transfer position during measurement, and the second crossbeam is fixedly arranged at an upper position corresponding to the second reagent aspiration position and the transfer position during measurement.

3. The sample analysis device according to claim 2, wherein:

A linear guide is provided on both sides of the first crossbeam along the long axis direction thereof, and the two first reagent needles are respectively provided on the linear guides on both sides of the first crossbeam, and the first reagent needles move linearly between the first reagent aspiration position and the incubation transfer position along the linear guides on which they are located;

The second crossbeam is provided with a linear guide rail on both sides along its long axis direction, and the two second reagent needles are respectively arranged on the linear guide rails on both sides of the second crossbeam. The second reagent needles move linearly between the second reagent aspiration position and the measurement transfer position along the linear guide rails.

4. The sample analysis device according to any one of claims 1 to 3, characterized in that:

The first reagent dispensing component further includes two second drive components that independently drive the two first reagent needles to perform linear motion, and the two second drive components' respective independent driving force output ends act on the two first reagent needles respectively, so as to drive the two first reagent needles to perform linear motion between the first reagent aspiration position and the incubation transfer position along the long axis direction of the first crossbeam;

The second reagent dispensing component also includes two third driving components that are different from the second driving components and independently drive the two second reagent needles to perform linear motion, and the two independent driving force output ends of the two third driving components act on the two second reagent needles respectively to drive the two second reagent needles to perform linear motion along the long axis direction of the second beam between the second reagent aspiration position and the measurement transfer position.

5. The sample analysis device according to any one of claims 1 to 3, characterized in that the sample injection component, the reaction cup loading component, the reaction component and the measurement component are arranged around the reagent carrying component.

6. The sample analysis device according to any one of claims 1 to 3, characterized in that the reaction component and the measurement component are arranged adjacent to each other around the reagent carrying component.

7. The sample analysis device according to any one of claims 1 to 3, characterized in that the transfer position during incubation is arranged between the reagent carrying component and the reaction component; the transfer position during measurement is arranged between the reagent carrying component and the measurement component.

8 . The sample analysis device according to claim 5 , wherein the reaction component is rectangular and has a plurality of reaction cup placement positions; and the measurement component is rectangular and has a plurality of reaction cup placement positions.

9 . The sample analysis device according to claim 8 , wherein the length direction of the reaction component is arranged along a first direction, and the length direction of the measurement component is arranged along a second direction different from the first direction.

10. The sample analysis device according to claim 9, wherein:

The trajectory of the linear motion of the two first reagent needles of the first reagent dispensing component between the first reagent aspiration position and the incubation transfer position is the first motion trajectory;

The trajectory of the linear motion of the two second reagent needles of the second reagent dispensing component between the second reagent aspirating position and the measurement transfer position is the second motion trajectory;

The first motion trajectory does not intersect with the second motion trajectory.

11. The sample analysis device according to claim 10, wherein:

The incubation transfer position includes a first position and a second position for placing a reaction cup, one of the first reagent needles moves linearly along the first crossbeam between the first reagent suction position and the first position, and the other first reagent needle moves linearly along the first crossbeam between the first reagent suction position and the second position;

The transfer position in the assay includes a third position and a fourth position for placing a reaction cup, one of the second reagent needles moves linearly along the second beam between the second reagent aspiration position and the third position, and the other second reagent needle moves linearly along the second beam between the second reagent aspiration position and the fourth position.

12 . The sample analysis device according to claim 11 , wherein the first reagent needle and/or the second reagent needle is provided with a heating component for heating the sucked reagent.

13. The sample analysis device as claimed in claim 12, characterized in that the first reagent dispensing component also includes a first Z-direction driving assembly for driving the first reagent needles in the first group of reagent needles to move in the vertical direction respectively, and the number of the first Z-direction driving assemblies is the same as the number of the first reagent needles in the first group of reagent needles; the first Z-direction driving assemblies each include: a first Z-direction guide for guiding the first reagent needle to move in the vertical direction, and a first Z-direction driving member for driving the first reagent needle to move along the first Z-direction guide; the first reagent needle forms a sliding connection with the first crossbeam through the first Z-direction guide and the first Z-direction driving member, so that the first reagent needle can move in the vertical direction relative to the first crossbeam under the drive of the first Z-direction driving member;

The second reagent dispensing component also includes a second Z-direction drive assembly for driving the second reagent needles in the second group of reagent needles to move in the vertical direction respectively, and the number of the second Z-direction drive assemblies is the same as the number of the second reagent needles in the second group of reagent needles; the second Z-direction drive assemblies include: a second Z-direction guide for guiding the second reagent needle to move in the vertical direction, and a second Z-direction drive for driving the second reagent needle to move along the second Z-direction guide; the second reagent needle forms a sliding connection with the second beam through the second Z-direction guide and the second Z-direction drive, so that the second reagent needle can move in the vertical direction relative to the second beam under the drive of the second Z-direction drive.

14. A sample analysis device, comprising:

A reagent carrying component, used for carrying a reagent container and capable of rotating and rotating the reagent container to a reagent suction position;

One or more processing units; the processing unit is used to receive a reaction cup carrying a sample and process the sample in the reaction cup; each processing unit is configured with a corresponding reagent adding transfer position;

One or more reagent dispensing components, the number of the reagent dispensing components is equal to the number of the processing units, and one reagent dispensing component corresponds to one processing unit; each reagent dispensing component comprises: two reagent needles, a guide assembly for guiding the two reagent needles to make linear motion, and a second driving assembly for driving the two reagent needles to make linear motion along the guide assembly, wherein the guide assembly of each reagent dispensing component comprises: a crossbeam, and two guide members arranged in parallel and along the length direction of the crossbeam, the crossbeam is arranged along the direction determined by the reagent aspiration position and the reagent adding transfer position of the processing unit corresponding to the reagent dispensing component, and the guide member is a linear guide rail; the guide assembly is arranged along the direction determined by the reagent aspiration position and the reagent adding transfer position of the processing unit corresponding to the reagent dispensing component, so that the reagent needle absorbs the reagent from the reagent aspiration position and discharges it into the reaction cup at the reagent adding transfer position of the processing unit corresponding to the reagent dispensing component; and

The scheduling component is used to schedule the reaction cups that have completed the sample loading and are located in the sample loading position to each processing unit according to the detection process.

15. The sample analysis device as claimed in claim 14, characterized in that the crossbeam of the guide assembly in each reagent dispensing component is fixedly arranged above the reagent aspirating position and the reagent adding transfer position of the processing unit of the corresponding reagent dispensing component.

16. The sample analysis device as described in claim 14 is characterized in that the two linear guides are respectively arranged on both sides of the beam along the long axis direction of the beam, and the two reagent needles are respectively arranged on the linear guides on both sides of the beam and are slidably connected to the linear guides; the reagent needles move linearly between the reagent aspiration position and the reagent addition transfer position along the linear guides on which they are located.

17. The sample analysis device according to any one of claims 14 to 16, characterized in that:

The number of second drive components in each reagent dispensing part is equal to the number of reagent needles, and the independent driving force output ends of the multiple second drive components act on the multiple reagent needles accordingly, so as to independently drive the multiple reagent needles to move linearly along the guide component between the reagent aspiration position and the reagent addition transfer position.

18. The sample analysis device according to claim 17, wherein the linear motion trajectories of the reagent needles of different reagent dispensing components do not intersect with each other.

19. The sample analysis device according to any one of claims 14 to 16, wherein the reagent needles in the same reagent dispensing component are used to draw the same type of reagents.

20. The sample analysis device according to any one of claims 14 to 16, wherein the reagent adding transfer position of at least one of the processing units comprises reaction cup placement positions having the same number of reagent needles as that of the reagent dispensing component corresponding to the processing unit.

21. The sample analysis device according to any one of claims 14 to 16, characterized in that each reagent needle is provided with a heating component for heating the reagent drawn into the reagent needle.

22. The sample analysis device according to any one of claims 14 to 16, wherein at least one of the processing units is a reaction component for incubating a sample.

23. The sample analysis device according to any one of claims 14 to 16, wherein at least one of the processing units is a measuring component for measuring a sample.

24. The sample analysis device according to any one of claims 14 to 16, characterized in that the number of the reagent aspiration positions is the same as the number of reagent needles.

25. A sample analysis method, comprising the following steps:

A reaction cup loading step, controlling the reaction cup loading component to supply and carry the empty reaction cup;

A sample feeding step, controlling the sample feeding component to dispatch the sample rack carrying the sample to the sample suction position;

The sample dispensing step controls the sample dispensing component to absorb the sample from the sample aspiration position and dispense it into the reaction cup;

The first reagent dispensing step is to control the driving component to drive the reagent carrying component to rotate so that the reagent container carrying the first reagent is located at the first reagent aspirating position; control the two reagent needles provided on the first crossbeam on the first reagent dispensing component to absorb the first reagent in the reagent container through the first reagent aspirating position, and make a linear motion between the first reagent aspirating position and the reagent adding transfer position of the reaction component, so as to dispense the first reagent into the reaction cup at the reagent adding transfer position of the reaction component;

Incubation step, controlling the scheduling component to schedule the reaction cup after the first reagent is dispensed to the reaction component for incubation, and scheduling the reaction cup after the incubation to the reagent adding transfer position of the measurement component;

The second reagent dispensing step comprises controlling the reagent carrying component to rotate so that the reagent container carrying the second reagent is located at the second reagent aspirating position; controlling the two reagent needles on the second crossbeam on the second reagent dispensing component to aspirate the second reagent in the reagent container through the second reagent aspirating position, and to make a linear motion between the second reagent aspirating position and the reagent adding intermediate transfer position of the measuring component, so as to dispense the second reagent into the reaction cup at the reagent adding intermediate transfer position of the measuring component;

In the determination and recovery step, the control scheduling component dispatches the reaction cup after the second reagent is dispensed to the determination component for project detection, and dispatches the reaction cup after the detection to the waste recovery device.

26. The method according to claim 25, wherein the first reagent dispensing step further comprises:

The two first reagent needles of the first reagent dispensing component are controlled to independently move linearly between the first reagent aspirating position and the reagent adding transfer position of the reaction component.

27. The method according to claim 25 or 26, characterized in that the second reagent dispensing step further comprises:

The two second reagent needles of the second reagent dispensing component are controlled to move linearly between the second reagent aspirating position and the reagent adding transfer position of the measuring component independently of each other.

28. The method according to claim 27, wherein the first reagent dispensing step further comprises:

Each first reagent needle in the first reagent dispensing component is controlled to perform multiple preset actions in sequence to complete the first reagent adding operation, and among the multiple preset actions between two first reagent needles, at least one corresponding preset action does not overlap in time sequence.

29. The method according to claim 28, wherein the second reagent dispensing step further comprises:

Each second reagent needle in the second reagent dispensing component is controlled to perform multiple preset actions in sequence to complete the second reagent adding operation, and among the multiple preset actions between two second reagent needles, at least one corresponding preset action does not overlap in timing.