CN113996364A - Sampling device and sample processing device - Google Patents
Sampling device and sample processing device Download PDFInfo
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- CN113996364A CN113996364A CN202010747699.3A CN202010747699A CN113996364A CN 113996364 A CN113996364 A CN 113996364A CN 202010747699 A CN202010747699 A CN 202010747699A CN 113996364 A CN113996364 A CN 113996364A
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- 238000005070 sampling Methods 0.000 title claims abstract description 308
- 238000012545 processing Methods 0.000 title claims abstract description 34
- 238000007789 sealing Methods 0.000 claims description 34
- 239000007788 liquid Substances 0.000 claims description 6
- 230000002093 peripheral effect Effects 0.000 claims description 6
- 239000012472 biological sample Substances 0.000 abstract description 87
- 239000000523 sample Substances 0.000 abstract description 55
- 238000004891 communication Methods 0.000 abstract description 6
- 206010036790 Productive cough Diseases 0.000 description 14
- 210000003802 sputum Anatomy 0.000 description 14
- 208000024794 sputum Diseases 0.000 description 14
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- 208000015181 infectious disease Diseases 0.000 description 9
- 238000012864 cross contamination Methods 0.000 description 8
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- 239000012530 fluid Substances 0.000 description 6
- 238000010586 diagram Methods 0.000 description 5
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- 235000011389 fruit/vegetable juice Nutrition 0.000 description 5
- 230000002458 infectious effect Effects 0.000 description 5
- 244000000010 microbial pathogen Species 0.000 description 5
- 208000035473 Communicable disease Diseases 0.000 description 4
- 230000004308 accommodation Effects 0.000 description 4
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- 229910052782 aluminium Inorganic materials 0.000 description 1
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
- B01L3/02—Burettes; Pipettes
- B01L3/0289—Apparatus for withdrawing or distributing predetermined quantities of fluid
- B01L3/0293—Apparatus for withdrawing or distributing predetermined quantities of fluid for liquids
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- Chemical & Material Sciences (AREA)
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Abstract
本发明公开了一种采样装置及样本处理装置，该采样装置包括第一容器，第一容器内设有第一容纳腔和采样槽，第一容纳腔与采样槽连通；采样装置还包括采样组件，采样组件用于设在第一容器内；在采样位置时，采样组件阻止第一容纳腔与采样槽连通，并使采样槽与第一容器的外部连通。本发明的采样装置通过在第一容器内设置第一容纳腔和采样槽，先使生物样本通过第一容纳腔进入采样槽内，由于采样槽具有固定的容积，通过采样组件将采样槽内的生物样本取出即可得到固定体积的生物样本，使得采样人员在无需观察的情况下就可操作，不会出现采样的体积误差，有效提高了采样人员的采样效率。
The invention discloses a sampling device and a sample processing device. The sampling device comprises a first container, wherein a first accommodating cavity and a sampling groove are arranged in the first container, and the first accommodating cavity is communicated with the sampling groove; the sampling device further comprises a sampling assembly , the sampling assembly is arranged in the first container; in the sampling position, the sampling assembly prevents the communication between the first accommodating cavity and the sampling groove, and makes the sampling groove communicate with the outside of the first container. In the sampling device of the present invention, a first accommodating cavity and a sampling groove are arranged in the first container, and the biological sample is firstly entered into the sampling groove through the first accommodating cavity. A biological sample of a fixed volume can be obtained by taking out the biological sample, so that the sampler can operate without observation, and there will be no volume error of sampling, which effectively improves the sampling efficiency of the sampler.
Description
Technical Field
The invention relates to the field of medical instruments, in particular to a sampling device and a sample processing device.
Background
Clinically, when a biological sample (e.g., sputum) needs to be tested, a non-fixed volume of sputum is usually manually aspirated into a sampling tube, and a corresponding volume of treatment fluid (digestive juice) is added to each sputum for treatment. However, when the number of samples to be detected is large, it is difficult to add a processing liquid in a volume different from that of each sample, and it is generally performed by using a processing liquid in a fixed volume, which requires that the sample volume of each sample tube be uniform. For example, if the optimal volume ratio of sputum to digestive juice is 1:2, and the volume of digestive juice in each sampling tube is fixed to 2mL, the amount of sputum sample to be loaded in each sampling tube should be 1 mL. Because the error of sucking the biological sample by the manual sucking method is large, the volume sucked every time is possibly different, and the accuracy of sample detection is affected, a device capable of quantitatively collecting the biological sample is needed.
Disclosure of Invention
The present invention is directed to overcome the above-mentioned drawbacks of the prior art, and provides a sampling device and a sample processing device.
The invention solves the technical problems through the following technical scheme:
a sampling device comprises a first container, wherein a first accommodating cavity and a sampling groove are arranged in the first container, and the first accommodating cavity is communicated with the sampling groove;
the sampling device further comprises a sampling component, wherein the sampling component is arranged in the first container;
in the sampling position, the sampling assembly prevents the first receiving chamber from communicating with the sampling slot and allows the sampling slot to communicate with the exterior of the first container.
In this scheme, through set up first chamber and the sampling groove of holding in first container, make earlier that the biological sample who treats the inspection gets into the sampling inslot through first chamber that holds, because the sampling groove has fixed volume, take out the biological sample in the sampling inslot through the sampling subassembly and can obtain the biological sample of fixed volume.
Preferably, the sampling groove is arranged below the first accommodating cavity, and an upper end opening of the sampling groove is connected with a lower end opening of the first accommodating cavity;
a first through hole is formed in the bottom of the sampling groove, and a first sealing element used for sealing the first through hole is arranged on the first through hole.
In this scheme, adopt above-mentioned structural style, the sampling groove to establish the first below that holds the chamber does benefit to the first biological sample that holds the intracavity and flows into the sampling inslot, makes the interior biological sample that fills up of sampling groove, guarantees the uniformity of sample volume at every turn. Open the bottom in sampling groove and set up first through-hole and first sealing member, the sampling subassembly of being convenient for opens the sampling groove, makes the biological sample in the sampling groove flow out.
Preferably, the sampling position includes a first position, the first position is located on a plane where the upper end opening of the sampling groove is located, and the sampling assembly moves to the first position first, so that the first accommodating cavity is not communicated with the sampling groove.
Preferably, the sampling assembly comprises a push rod and a piston head, the piston head is arranged at one end of the push rod, and the outer peripheral surface of the piston head can be attached to the inner side wall of the sampling groove;
the piston head can be used for preventing the sample in the first containing cavity from entering the sampling groove, and the piston head is also used for sending the sample in the sampling groove out of the first container.
In this scheme, adopt the sampling subassembly of above-mentioned structure, simple structure, the operation of being convenient for use the piston head propelling movement biological sample, can see off the biological sample in the sampling groove as far as, reduce the residue of the biological sample in the sampling groove, guarantee the volume uniformity of biological sample when detecting.
Preferably, the sampling assembly further comprises a puncturing element arranged on one side of the piston head opposite to the push rod, and the puncturing element is used for opening the sampling groove so as to enable the sampling groove to be communicated with the outside of the first container.
In this scheme, increase the piercing depth at the tip of piston head, be convenient for open the sampling groove fast, reduce the resistance of piston head at the inslot removal of sampling for the sample in the sampling groove flows out fast, improves the efficiency of sample.
Preferably, a guide member is arranged above the sampling groove, the guide member is provided with a guide channel, and the sampling assembly is arranged in the guide channel and can move along the axial direction of the guide channel.
In this scheme, set up the guide on the one hand and can provide the guide effect for the sampling subassembly for the piston head can get into the sampling groove smoothly, and on the other hand provides the support for the sampling subassembly, avoids the sampling subassembly to take place crooked, influences the operation.
Preferably, the sampling device further comprises an upper cover, the upper cover is provided with a second through hole, and an upper end opening of the guide channel is communicated with the second through hole.
In this scheme, set up the upper cover and can seal the biological sample who treats the sampling in first container for the biological sample is in a inclosed environment at the sampling in-process, effectively stops cross contamination and the biological safety risk that infectious pathogenic microorganism loss brought between the biological sample.
A sample processing device comprising a sampling device as described above and a second container having a second receiving chamber, the cavity of the second receiving chamber being communicable with the sampling slot.
In this scheme, directly send into the biological sample in the sampling groove in the cavity that the second held the chamber for biological sample's processing is in airtight environment, has effectively stopped cross contamination and infectious disease microorganism loss between the biological sample, has guaranteed sample treatment's accuracy and security.
Preferably, the second containing cavity is pre-filled with a sample processing liquid.
In this scheme, hold intracavity pre-installation sample treatment fluid at the second, avoid holding the intracavity towards the second again and add sample treatment fluid after the sample for biological sample's sample aftertreatment is in encapsulated situation, has effectively stopped cross contamination and infectious disease microorganism loss between the biological sample, has guaranteed sample treatment's accuracy and security, also is favorable to the automation mechanized operation of sample test.
Preferably, the second container is further provided with a sampling channel, and the sampling channel is communicated with the inside of the second accommodating cavity and the outside of the second container;
and a second sealing piece is arranged on the sampling channel and used for sealing the sampling channel so as to isolate the inside of the second containing cavity from the outside of the second container.
In this scheme, set up sampling channel and be convenient for hold the intracavity to the second and add sample treatment fluid or be convenient for hold the intracavity good biological sample of processing with the second and take out and carry out follow-up operation.
On the basis of the common knowledge in the field, the above preferred conditions can be combined randomly to obtain the preferred embodiments of the invention.
The positive progress effects of the invention are as follows: according to the sampling device, the first containing cavity and the sampling groove are arranged in the first container, a biological sample to be tested enters the sampling groove through the first containing cavity, and the sampling groove has a fixed volume, so that the biological sample with the fixed volume can be obtained by taking out the biological sample in the sampling groove through the sampling assembly, a sampling person can operate without observation, sampling volume errors can not occur, and the sampling efficiency of the sampling person is effectively improved.
Drawings
Fig. 1 is a schematic structural diagram of a sampling apparatus in which a sampling assembly is in a non-sampling position in embodiment 1 of the present invention.
Fig. 2 is a schematic structural diagram of the sampling apparatus when the sampling assembly is just moved to the sampling position in embodiment 1 of the present invention.
Fig. 3 is a schematic structural diagram of the sampling apparatus in embodiment 1 of the present invention, in which the sampling assembly is in the sampling position.
Fig. 4 is a schematic structural view of a first container in embodiment 1 of the present invention.
Fig. 5 is a sectional view of a first container in embodiment 1 of the present invention.
FIG. 6 is a schematic structural diagram of a sampling assembly in embodiment 1 of the present invention
Fig. 7 is a schematic structural view of an upper cover in embodiment 1 of the present invention.
Fig. 8 is a schematic structural view of a sample processing device according to embodiment 1 of the present invention.
Fig. 9 is a sectional view of a sample processing device according to example 1 of the present invention.
Fig. 10 is a schematic structural view of a second container in embodiment 1 of the present invention.
FIG. 11 is a flowchart of a method for sampling a biological sample in example 1 of the present invention.
FIG. 12 is a schematic structural diagram of a sampling device in embodiment 2 of the present invention
Fig. 13 is a schematic structural view of a first container in embodiment 2 of the present invention.
Description of reference numerals:
First via hole 103
Second via 201
Fourth via 202
Push rod 310
Piercing member 330
Pressing part 340
Detailed Description
The present invention is further illustrated by the following examples, but is not limited thereby in the scope of the following examples.
Example 1
As shown in fig. 1 to 7, a sampling device according to the present embodiment includes a first container 100, a first accommodating chamber 101 and a sampling slot 102 are provided in the first container 100, and the first accommodating chamber 101 is communicated with the sampling slot 102; the sampling device further comprises a sampling assembly 300, the sampling assembly 300 being adapted to be disposed within the first container 100; in the sampling position, sampling assembly 300 prevents first receiving chamber 101 from communicating with sampling slot 102 and allows sampling slot 102 to communicate with the exterior of first container 100.
The sampling position comprises a first position, the first position is located on the plane where the upper end opening of the sampling groove 102 is located, and the sampling assembly 300 is firstly moved to the first position to enable the first containing cavity 101 to be not communicated with the sampling groove 102.
The working process of the sampling device is specifically shown in fig. 1-3, wherein fig. 1 is a schematic view of the sampling device in a non-sampling state, in which the sampling assembly 300 is in a non-sampling position, i.e., above the notch 212; FIG. 2 is a schematic view of the sampling device just after the sampling assembly 300 has been moved to the sampling position, where the sampling assembly 300 has just separated the first receiving chamber 101 from the sampling slot 102, i.e., the first position described above; fig. 3 is a schematic view of the sampling device in a sampling state, in which the sampling assembly 300 communicates the sampling slot 102 with the exterior of the first container 100.
The sampling device of this embodiment is through setting up first chamber 101 and the sampling groove 102 of holding in first container 100, makes the biological sample that waits to examine earlier get into the sampling groove 102 through first chamber 101 of holding, because the sampling groove 102 has fixed volume, takes out the biological sample in the sampling groove 102 through sampling subassembly 300 and can obtain the biological sample of fixed volume for the sampling personnel just can operate under the condition that need not to observe, the volume error of sampling can not appear, sampling personnel's sampling efficiency has effectively been improved.
Sampling groove 102 is provided below first accommodation chamber 101, and the upper end opening of sampling groove 102 is connected with the lower end opening of first accommodation chamber 101. As shown in fig. 1 to 5, a sampling groove 102 is formed in the bottom of the first accommodating chamber 101, and the structure of the first accommodating chamber 101 is similar to a funnel shape, so that the biological sample added into the first accommodating chamber 101 flows into the sampling groove 102 at the bottom, so that the sampling groove 102 is filled with the biological sample, and the consistency of sampling volume each time is ensured.
The sampling tank 102 may be designed to have a fixed volume as required to ensure the consistency of the volume of biological samples collected during the sampling process. Considering that the biological sample to be collected may have a certain viscosity, some biological samples may stick to the inner wall of the sampling tank 102, the volume of the sampling tank 102 may be designed to be larger than the volume of the sampling tank 102, for example, the required volume of the biological sample is 2mL, and the volume of the sampling tank 102 may be designed to be 2.1 mL.
As shown in fig. 1-3, in the present embodiment, a first through hole 103 is formed at the bottom of the sampling slot 102, and a first sealing member 104 for sealing the first through hole 103 is disposed on the first through hole 103. The first through hole 103 is formed to communicate the sampling groove 102 with the outside of the first container 100, and the inner bottom surface 1021 of the sampling groove 102 is set to be an inclined surface, so that the biological sample in the sampling groove 102 can flow out of the sampling groove 102, and the residue of the biological sample in the sampling groove 102 is reduced. Before the device is used, the first through hole 103 is sealed by the first sealing element 104, so that the first through hole 103 is in a closed state. In this embodiment, the first sealing member 104 is made of aluminum foil and is fixed outside the first through hole 103 by heat melting.
In other embodiments, the inner bottom surface 1021 of the sampling slot 102 can also be a flat or curved surface. The first sealing member 104 may be a water-impermeable and stable material such as plastic film, rigid plastic, metal, etc. and is fixed outside the first through hole 103 by heat melting, gluing, etc. so as to isolate the sampling slot 102 from the outside of the first container 100.
Alternatively, in other embodiments, the bottom of the sampling groove 102 is not machined with the first through hole 103, and the bottom of the sampling groove 102 is a thin layer integrally formed with the sample groove 112, which makes the sampling groove 102 isolated from the outside of the first container 100 and is easy to fall off or be punctured by an external force.
The sampling assembly 300 comprises a push rod 310 and a piston head 320, wherein the piston head 320 is arranged at one end of the push rod 310, and the outer peripheral surface of the piston head 320 can be attached to the inner side wall of the sampling groove 102; piston head 320 may be used to prevent a sample in first receiving chamber 101 from entering sampling slot 102, and piston head 320 may also be used to transport a sample in sampling slot 102 out of first container 100.
The sampling assembly 300 further includes a piercing member 330, the piercing member 330 being disposed on a side of the piston head 320 opposite the push rod 310, the piercing member 330 being configured to open the sampling slot 102 to place the sampling slot 102 in communication with the exterior of the first container 100.
Specifically, as shown in fig. 1-3 and 6, the sampling assembly 300 of the present embodiment includes a push rod 310, a piston head 320, and a piercing member 330, wherein the piston head 320 is provided with the piercing member 330 on a side opposite to the push rod 310, and an outer peripheral surface of the piston head 320 can abut against an inner sidewall 1022 of the sampling slot 102.
In this embodiment, the piercing member 330 is used for piercing the first sealing member 104 to open the first through hole 103, the piston head 320 is used for preventing the sample in the first containing chamber 101 from entering the sampling slot 102 when the first sealing member 104 is pierced, and the piston head 320 is also used for sending the sample in the sampling slot 102 out of the first container 100. The sampling assembly 300 with the structure has a simple structure, is convenient to operate, can send out the biological sample in the sampling groove 102 as far as possible by pushing the biological sample by using the piston head 320, reduces the residue of the biological sample in the sampling groove 102, and ensures the volume consistency of the biological sample during detection.
Specifically, as shown in fig. 4 to 6, the inner side wall 1022 of the sampling slot 102 is circular, the outer circumferential surface of the piston head 320 is matched with the inner side wall 1022 of the sampling slot 102, and the piston head 320 is made of an elastic material, so that the sampling slot 102 and the first accommodating cavity 101 are sealed and isolated when the piston head 320 enters the sampling slot 102, and the biological sample in the first accommodating cavity 101 is prevented from entering the sampling slot 102.
As shown in fig. 6, the puncture device 330 includes a puncture tip 332 and a connection part 331, and the outer diameter of the connection part 331 is smaller than the maximum outer diameter of the puncture tip 332. This prevents the connecting portion 331 from re-blocking the first through hole 103 after the piercing member 330 pierces the first sealing member 104 of the first through hole 103, thereby facilitating the outflow of the biological sample from the sampling slot 102.
As shown in fig. 6, a flow path 333 is further provided on the outer peripheral surface of the puncture element 330, and the flow path 333 extends from the piston head 320 to the outer end of the puncture element 330 in the axial direction of the puncture element 330. Specifically, as shown in fig. 5, the puncturing element 330 is formed by combining four plates in a crossing manner, and a flow channel 333 is formed between each two plates. This not only prevents the connection portion 331 from re-sealing the first through hole 103 after the piercing member 330 pierces the first sealing member 104 of the first through hole 103, but also accelerates the flow of the biological sample out of the sampling well 102.
The length of the piercing member 330 is also provided with care so that when the sampling assembly 300 is in the non-sampling position, the end of the piercing member 330 distal to the piston head 320 is disposed within the sampling slot 102. It is avoided that the piercing member 330 opens the sampling slot 102 when the sampling assembly 300 has not isolated the first receiving chamber 101 from the sampling slot 102.
Of course, in other embodiments, the puncturing element 330 can be designed in other structures, for example, the first sealing element 104 is made of hard plastic, and the puncturing element 330 can be a cylinder structure with an outer diameter smaller than the inner diameter of the first through hole 103, so that the first through hole 103 can be opened without blocking the first through hole 103 by simply separating the hard plastic. Other structures of the piercing member 330 are not described herein, and only the first through hole 103 is required to be opened and not to block the first through hole 103.
Alternatively, in other embodiments, the piercing member 330 may not be provided, and the pressure generated by the movement of the piston head 320 in the sampling slot 102 may be used to disengage the first sealing member 104 from the first through hole 103.
As shown in fig. 1-3 and 7, the upper cover 200 is disposed on the first container 100, and the upper cover 200 can seal the biological sample to be sampled in the first container 100, so that the biological sample is in a sealed environment during sampling, thereby effectively avoiding cross contamination between biological samples and biological safety risk caused by the escape of infectious pathogenic microorganisms.
As shown in fig. 1 to 3, a guide 210 is disposed above the sampling slot 102, the guide 210 has a guide channel 211, and the sampling assembly 300 is disposed in the guide channel 211 and is movable along the axial direction of the guide channel 211. The guiding element 210 is arranged to provide a guiding function for the sampling assembly 300, so that the piston head 320 can smoothly enter the sampling slot 102, and provide a support for the sampling assembly 300, so as to prevent the sampling assembly 300 from being inclined and affecting the operation.
Specifically, in this embodiment, the guide member 210 is integrally formed with the upper cover 200, the guide channel 211 is located directly above the sampling slot 102, the inner side wall of the guide channel 211 is the same as the inner diameter of the inner side wall 1022 of the sampling slot 102, the upper end opening of the guide channel 211 is communicated with the second through hole 201 on the upper cover 200, the lower end portion of the guide channel 211 abuts against the upper end surface of the opening of the sampling slot 102, the lower end of the guide member 210 is provided with a notch 212 for allowing the biological sample in the first accommodating cavity 101 to enter the sampling slot 102, and the notch 212 is also used for allowing the biological sample outside the sampling slot 102 and located in the guide channel 211 to flow back into the first accommodating cavity 101.
Before a sample is collected by the present sampling device, the piston head 320 of the sampling assembly 300 is positioned in the guide channel 211 above the notch 212, and at this time, the piston head 320 does not prevent the biological sample in the first receiving chamber 101 from entering the sampling slot 102. When sampling is performed, piston head 320 moves downward to block gap 212 and block the upper opening of sampling slot 102, thereby preventing the biological sample in first receiving chamber 101 from entering into sampling slot 102.
In order to avoid cross contamination between the biological sample in the first accommodating cavity 101 and the outside through the guide channel 211, the biological safety risk caused by the escape of infectious pathogenic microorganisms in the biological sample is prevented. A partition is further provided on the sampling assembly 300, and is provided in the guide passage 211, and the partition is used for preventing the first accommodating chamber 101 from communicating with the second through hole 201.
Referring to fig. 1-3 and fig. 6, in the present embodiment, a pressing portion 340 is disposed at the top end of the push rod 310, an outer peripheral surface of the pressing portion 340 is attached to an inner wall surface of the guide channel 211, and the pressing portion 340 moves along with the push rod 310, but is always located in the guide channel 211 and above the notch 212. Thus, the pressing part 340 is equivalent to a spacer that prevents the first receiving chamber 101 from communicating with the outside of the upper cover 200 through the guide passage 211. To ensure the isolation and sealing effect, an elastic material such as rubber may be provided on the outer circumferential surface of the pressing portion 340.
Of course, in other embodiments, the spacer may be separately disposed at a middle position of the push rod 310; alternatively, the height of the piston head 320 may be extended such that the piston head 320 may close the gap 212 after the bottom of the piston head 320 contacts the inner bottom surface of the sampling slot 102, and the piston head 320 may also serve as a spacer.
In this embodiment, the upper cap 200 is detachably coupled to the first container 100, and as shown in fig. 1 to 3, the upper cap 200 is screw-coupled to the first container 100. The detachable connection of the cover 200 to the first container 100 facilitates the manufacturing process on the one hand and the addition of the biological sample into the first containing chamber 101 on the other hand.
Of course, in other embodiments, the upper cover 200 and the first container 100 may be fixed by gluing or clipping.
Of course, when the biological sample is added to the first containing chamber 101, a third through hole communicating with the first containing chamber 101 may be opened in the upper cover 200, in addition to the opening of the upper cover 200 as in the present embodiment. So, can directly add biological sample towards first holding chamber 101 through the third through-hole on the upper cover 200, can greatly reduced biological sample contaminated and the risk of infectious disease microorganism loss in the biological sample, and need not to uncap when adding biological sample, be favorable to realizing biological sample's automated operation.
A sealing member for sealing the third through hole to isolate the inside of the first receiving chamber 101 from the outside of the upper cover 200 is required to be provided on the third through hole. Immediately after the biological sample is added to the first containing chamber 101, the third through hole is sealed by the sealing member.
As shown in fig. 8 to 9, the present embodiment further discloses a sample processing device, which includes the above sampling device and a second container 400, wherein the second container 400 has a second accommodating chamber 401, the second accommodating chamber 401 is disposed below the first accommodating chamber 101, and a cavity of the second accommodating chamber 401 can communicate with the sampling slot 102.
According to the sample processing device, the biological sample in the sampling groove 102 is directly sent into the cavity of the second accommodating cavity 401, so that the biological sample is processed in a closed environment, cross contamination among the biological samples and the escape of infectious pathogenic microorganisms are effectively avoided, and the accuracy and the safety of sample processing are ensured.
Specifically, as shown in fig. 8 to 9, in order to facilitate manufacturing, the sample processing device of the present embodiment is composed of a first container 100 and a second container 400, the first container 100 has a first accommodating chamber 101, the second container 400 has a second accommodating chamber 401, and the first container 100 and the second container 400 can be fixed by screwing, or by clipping, gluing, or the like.
In order to ensure the sealing property of the sample processing device, sealing rings are provided at the joints between the upper cover 200 and the first container 100 and between the first container 100 and the second container 400.
As shown in fig. 9, the first container 100 is further provided with a sampling passage 105, and the sampling passage 105 communicates the inside of the second receiving chamber 401 with the outside of the second container 400. The sampling passage 105 is provided to facilitate the addition of the sample processing liquid into the second containing chamber 401 and the extraction of the processed biological sample from the second containing chamber 401 for subsequent operations.
As shown in fig. 9, in the present embodiment, the sampling channel 105 is disposed on the first container 100, the lower end of the sampling channel 105 extends into the second accommodating chamber 401, the upper end of the sampling channel 105 is communicated with the fourth through hole 202 of the upper cap 200, and during sampling, the processed biological sample can be taken out from the sampling channel 105 directly through the fourth through hole 202 of the upper cap 200.
Of course, in other embodiments, a through hole may be directly formed in the sidewall or the bottom of the second container 400 to serve as a sampling port, which is not described herein again.
A second sealing member 203 is provided on the sampling passage 105, and the second sealing member 203 serves to seal the sampling passage 105 to isolate the inside of the second receiving chamber 401 from the outside of the second container 400. In this embodiment, as shown in fig. 8-9, the second sealing member 203 and the upper cover 200 are both made of plastic and are integrally formed during processing, and the second sealing member 203 can pass through the fourth through hole 202 and seal the upper end opening of the sampling passage 105. Of course, in other embodiments, the second seal 203 may be a separate component.
As shown in fig. 9 to 10, in the present embodiment, the inner bottom surface 403 of the second receiving chamber 401 is a slope inclined toward the side of the opening of the sampling passage 105 in the second receiving chamber 401. The inner bottom surface 403 of the second accommodating cavity 401 is set to be an inclined surface, so that the biological sample in the second accommodating cavity 401 is gathered towards one side of the sampling channel 105, the biological sample processed in the second accommodating cavity 401 can be taken out conveniently when an automatic instrument is adopted, and the residual quantity of the biological sample in the second accommodating cavity 401 can be effectively reduced.
As shown in fig. 8 and 10, the bottom of the second container 400 is provided with a recess 402, and the recess 402 is used for being matched and positioned with an external device. Set up depressed part 402 on the one hand and can carry on spacingly to second container 400, second container 400 takes place to empty when avoiding carrying out automated operation, and on the other hand can fix a position this sample processing device for a plurality of sample processing device's various passageways are all neatly corresponding to arrange, the automatic instrument's of being convenient for discernment and operation.
In this embodiment, the second receiving chamber 401 is filled with a sample processing liquid. For example, when the volume of the sampling tank 102 is 2mL and the optimal volume ratio of the biological sample to the corresponding sample treatment solution is 1:2, a sample treatment solution having a volume of 4mL may be previously loaded into the second accommodating chamber 401.
Through holding chamber 401 at the second and preassembling sample treatment fluid in, avoid holding chamber 401 at the second after the sample and adding sample treatment fluid towards the second again for the sample and the reaction of biological sample are in sealed environment, have effectively stopped cross contamination and infectious disease microorganism loss between the biological sample, have guaranteed accuracy and the security of sample processing.
As shown in fig. 11, the present embodiment also discloses a sampling method of a biological sample, which uses the sampling device as described above, and the sampling method includes the following steps:
s1, pouring the biological sample to be detected into the first accommodating cavity 101;
s2, enabling the biological sample to flow into the sampling groove 102 and fill the sampling groove 102;
s3, the sampling assembly 300 is pressed to make the biological sample in the sampling slot 102 flow out of the first container 100.
In step S3, the sampling method includes the following steps:
s31, moving the sampling assembly 300 to a sampling position to prevent the first accommodating cavity 101 from being communicated with the sampling groove 102;
s32, continuing to press the sampling assembly 300 to connect the sampling slot 102 with the exterior of the first container 100;
s33, the lower end of the sampling assembly 300 is pressed to the bottom of the sampling well, so that the biological sample in the sampling well 102 flows out of the first container 100.
The following further describes how to use the sample processing device of this embodiment to process a biological sample, which is a sputum as an example, wherein the volume of the sampling tank 102 is 2mL, and the optimal ratio of the sputum to the digestive juice is 1:2, with reference to the specific biological sample, the specific usage method is as follows:
s21, pre-filling 4mL of digestive juice in the second containing cavity 401;
s22, opening the upper cover 200, and pouring the sputum to be detected into the first accommodating cavity 101, wherein the volume of the poured sputum is more than 2 mL;
s23, covering the upper cover 200, and filling the sampling groove 102 with the sputum after the sputum flows into the sampling groove 102;
s24, pressing down the sampling assembly 300, the first sealing member 104 being pierced by the piercing member 330, the sputum in the sampling slot 102 being squeezed into the second containing chamber 401 by the piston head 320;
s25, placing the sample processing device on an ultrasonic processor, and carrying out ultrasonic processing for 5 minutes;
s26, opening the sampling channel 105, sucking the processed sputum and placing the sputum on a full-automatic nucleic acid extractor for subsequent detection.
The sample processing device of this embodiment collects biological sample's collection, ration sample and handles as an organic whole, uses this device to make collection, ration sample and the processing of sample accomplish in a sealing device, effectively stops cross contamination's problem and the biological security problem that infectious pathogenic microorganism loss brought between the sample.
Example 2
As shown in fig. 12, the sampling device of the present embodiment has substantially the same structure as that of embodiment 1, and the main differences are that: the guide member 210 and the upper cover 200 are integrally formed, a certain distance is reserved between the lower end of the guide member 210 and the end surface of the upper end opening of the sampling groove 102, a communication passage 213 of the first accommodating cavity 101 and the sampling groove 102 is formed, at this time, a notch 212 is not required to be arranged on the guide member 210, and the communication passage 213 ensures that the guide member 210 does not prevent the biological sample in the first accommodating cavity 101 from entering the sampling groove 102; communication channel 213 may also allow excess biological sample outside of sampling well 102 to be separated from sampling well 102 when sampling assembly 300 is in the sampling position.
Example 3
As shown in fig. 13, the sampling device of the present embodiment has substantially the same structure as that of embodiment 1, and the main differences are that: in the present embodiment, the guide 210 is provided on the first container 100, integrally formed with the first container 100. In this embodiment, the opening of the lower end of the guide channel 211 of the guide member 210 corresponds to the opening of the sampling slot 102, the lower end of the guide channel 211 is opened with a notch 212 communicating the first accommodating chamber 101 and the sampling slot 102, the notch 212 is used for allowing the biological sample in the first accommodating chamber 101 to enter the sampling slot 102, and is also used for allowing the biological sample outside the sampling slot 102 and in the guide channel 211 to flow back into the first accommodating chamber 101.
The upper end opening of the guide passage 211 is communicated with the second through hole 201 of the upper cover 200, and in order to ensure the sealing property, the connection part of the upper end opening of the guide passage 211 and the second through hole 201 needs to be sealed.
While specific embodiments of the invention have been described above, it will be appreciated by those skilled in the art that this is by way of example only, and that the scope of the invention is defined by the appended claims. Various changes and modifications to these embodiments may be made by those skilled in the art without departing from the spirit and scope of the invention, and these changes and modifications are within the scope of the invention.
Claims (10)
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