CN113995744B - A composition and its antibacterial application - Google Patents

Abstract

The inhibition of organic acids inhibits the next generation of bacteria by entering the bacterial cells in a free diffusion manner. The composition of the present invention can control the number of bacteria to a degree harmless to the human body. The invention relates to a composition and its antibacterial application, used for human body and environment application, can reduce the reproduction trend of its existing microorganism, the composition comprises: 1.2% malic acid, 0.3% hippuric acid, 0.1% fumaric acid, 0.1% glycine and water. Wherein the minimum inhibitory concentration of the composition for inhibiting escherichia coli and staphylococcus aureus is 2.5 times lower than that of the composition for inhibiting escherichia coli and staphylococcus aureus by using 1.2% of malic acid, 0.3% of hippuric acid, 0.1% of fumaric acid or 0.1% of glycine alone.

Description

A kind of composition and its antibacterial application

technical field

The invention relates to the technical field of medicine, in particular to a composition and its antibacterial application.

Background technique

Pangolin is used in the "Xianfang Huomingyin" in "Women's Prescription" written by Chen Ziming in the Song Dynasty. The effects of the whole prescription include clearing away heat and detoxification, reducing swelling and ulceration, promoting blood circulation and relieving pain, etc., and local redness, swelling, heat and pain. . Li Shizhen's "Compendium of Materia Medica" in the Ming Dynasty records that pangolins "eliminate phlegm, malaria, cold and heat, wind numbness, stiffness and pain, open the meridians, relieve pain and swelling, expel pus and blood, clear the orifices and kill insects." Divide pangolin and clam powder into equal parts, stir fry and grind into powder. From the above records in ancient medical books, we can see that pangolins are used to treat some diseases that modern medicine believes are caused by bacterial infections. Guo Yi et al reported in the "Journal of Hunan University of Traditional Chinese Medicine" that pangolin decoction has antibacterial effect on 11 kinds of bacteria including Escherichia coli, and the main component of pangolin decoction is squamous scales.

However, because pangolins are extremely endangered in modern society, eight species of pangolins have been included in the 2014 Red List of Endangered Species by the International Union for Conservation of Nature (IUCN), and in 2016 they were included in the CITES Appendix I protection of the Washington Convention. Animals, global trade banned. my country is a party to the Convention on International Trade in Endangered Species of Wild Fauna and Flora (CITES). At the same time, in 2020, the "Chinese Pharmacopoeia" will exclude pangolins from being used as medicine. Therefore, the present invention explores the ingredients or composition of ingredients in pangolin scales that inhibit the flora, and artificially synthesizes them as a substitute, so as to ensure that the relevant treatment plan that replaces the ingredients of the pangolin is not affected, and at the same time it can protect endangered species. Animal pangolin.

In the prior art, organic acid is used as an organic acid additive in various fields, among which citric acid is the most frequently used organic acid for antisepsis and flavor addition. The Chinese patent with publication number CN108670897B relates to a high-efficiency antibacterial wet tissue composition and a preparation method thereof. The raw materials for the preparation of the high-efficiency antibacterial wet tissue composition include, in parts by weight, 1-6 ethanol, 0.5-3 butanediol, 0.2-0.6 solubilizer, 0.01-0.02 menthol, 0.1-0.12 essence, and tea extract 0.5 to 1.0, olive leaf extract 0.5 to 1.0, nano silver, purslane extract 0.5 to 1.0, citric acid 0.1 to 0.2, sodium citrate 0.05 to 0.15, phenoxyethanol 0.01 to 0.05. The component of the composition involves the organic acid citric acid, and uses it as an antibacterial component, wherein phenoxyethanol is used for the solubilization of the citric acid. However, the existing organic acids are various, and the antibacterial effect of citric acid cannot reach the highest antibacterial efficiency among them.

The Chinese patent with the publication number CN108904374A relates to an oral cleanser, which includes: 3-8 parts by weight of tea extract, 1-3 parts of persimmon astringent, 1-2 parts of fructose, 2-4 parts of citric acid, spices 1-3 servings.

Generally speaking, the pH value of mouthwash or other oral cleaning agents should be in the range of 6.5-7.5, and the pH value of this range tends to be neutral. In a neutral environment, the use of mouthwash will not destroy the protein in the oral cavity, and Can play a very good bactericidal effect. Glycine buffer consisting of glycine is able to maintain liquid in the range of 2.2-10.6. Pseudomonas aeruginosa and Serratia marcescens are immunocompromised opportunistic pathogens that frequently occur in wounds. The oral cleanser prevents Pseudomonas aeruginosa and Serratia marcescens from entering the respiratory tract and causing harm to patients by inhibiting the growth of Pseudomonas aeruginosa and Serratia marcescens in the oral cavity.

In the prior art, the antibacterial and antiseptic effects of various weak acids have been confirmed, but the application of organic acids targeting the inhibition of Escherichia coli and Staphylococcus aureus, which have the most extensive and frequent impact on human diseases, has not been found.

In this experiment, through the study of the composition of pangolin scales with good antibacterial effect, the composition of metabolites used for antibacterial in pangolin scales was explored, so as to realize the antibacterial effect of multi-combination weak acids instead of pangolin scales.

In addition, on the one hand, due to differences in the understanding of those skilled in the art; The present invention does not possess the characteristics of these prior art, on the contrary, the present invention already possesses all the characteristics of the prior art, and the applicant reserves the right to add relevant prior art to the background technology.

Contents of the invention

Aiming at the deficiencies of the prior art, the invention provides a composition. When used on the human body or the environment in which the human body is located, it can reduce the reproduction tendency of the microorganisms present in said environment. The composition comprises: one or more of malic acid, hippuric acid, fumaric acid, glycine, succinic acid, L-valine and citric acid.

Preferably, the ingredients in the composition comprise: (1) malic acid; (2) hippuric acid; (3) optionally at least one of the following weakly acidic ingredients: 1) glycine; 2) succinic acid and/or L-valeric acid acid.

Preferably, the ingredients in the composition comprise: (1) malic acid; (2) hippuric acid; (3) optionally at least one of the following weakly acidic ingredients: 1) fumaric acid and/or glycine; 2) succinic acid and/or L-valine.

According to a preferred embodiment, the first component composed of malic acid, hippuric acid, succinic acid, and L-valine is formulated together with the second component composed of fumaric acid or succinic acid to form a Compositions to achieve an inhibitory effect.

The advantage of this technical solution: compared with the application of a single organic acid, the combination of organic acids formed by multiple combinations can form a better bacteriostatic effect than a single organic acid (confirmed by bacteriostatic tests), and through a variety of The combination of organic acids forms different flavors of disinfectant ingredients that are suitable for food, cosmetics, oral cleaning, etc. to act on the human body, achieving the purpose of deodorization and refreshing. The formation and decomposition of organic acids are harmless to the human body and the environment. The principle of antibacterial and sterilizing is to enter the bacteria in the form of small molecules and destroy the alkaline active environment in the bacteria, thereby inhibiting the activity of the bacteria. The combination of organic acids of multiple combinations forms a covalent structure with each other, and forms a mutual augmented bacteriostatic effect, and replaces the organic acids that are randomly combined in a single or polymorphic way and require a large dose of addition with a smaller dose of the composition, thereby Reduce the harm of excessive intake of food additives to the human body.

According to a preferred embodiment, said composition acting on the human body or the environment comprises malic acid, hippuric acid, glycine and citric acid, wherein the composition is based on the interaction of malic acid and citric acid being able to enhance the buffering of acidic senses For fruity ingredients that act on the environment.

According to a preferred embodiment, the composition comprises at least malic acid, hippuric acid, glycine and citric acid, wherein the components constituting the composition are applied to the skin surface in the form of a non-Newtonian fluid.

According to a preferred embodiment, the composition comprises at least malic acid, hippuric acid, glycine and fumaric acid, wherein the components constituting the composition are applied to the skin surface in the form of a non-Newtonian fluid.

The advantages of this technical solution: the drugs and cosmetics used for the repair, treatment or protection of the skin surface cannot achieve stable attachment on the face due to the fluidity of the fluid during the application process, but the paste drug form is due to the viscosity Not easy to apply evenly. The present invention adopts the component form of non-Newtonian fluid, which reduces its fluidity and does not change the viscosity, thereby ensuring the stability of the components on the face and skin.

According to a preferred embodiment, the composition can inhibit the division of Escherichia coli and Staphylococcus aureus in a synergistic manner.

A composition, which can constitute a cleaning component of an oral cleaning agent in the form of a dispersed phase and an abrasive, and the composition is used to inhibit the growth of Escherichia coli and Staphylococcus aureus in the oral cavity, wherein the composition contains malic acid , hippuric acid, fumaric acid, glycine, succinic acid, L-valine and citric acid in one or more.

A composition capable of inhibiting the propagation of Escherichia coli and Staphylococcus aureus on the surface of human skin or in food, wherein the composition contains malic acid, hippuric acid, fumaric acid, glycine, succinic acid, L - One or more of valine and citric acid.

Antibacterial application of a composition, which is applied as an additive in a detergent or a bactericide, said composition as an additive is provided with a first composition for releasing H ⁺ and for increasing the killing effect and time second composition. The first composition includes: one or more of malic acid, hippuric acid, fumaric acid, glycine, succinic acid, L-valine and citric acid, and the second composition includes hydrocolloid. Wherein, when the composition as an additive is applied, the first composition intervenes in the second composition, and accompanied by the second composition on the E. Staphylococcus encapsulation acts on the inside of Escherichia coli or Staphylococcus aureus.

An antibacterial application of a composition, characterized in that it is used as a drug for inhibiting the growth of bacteria in tissue inflammation caused by bacterial infection, and the main components of the drug include: malic acid, hippuric acid, fumaric acid, glycine, succinic acid One or more of acid, L-valine and citric acid.

Detailed ways

A detailed description will be given below in conjunction with the accompanying drawings.

The invention designs an antibacterial composition, which can realize the most effective inhibition on microorganisms represented by Escherichia coli and Staphylococcus aureus.

Organic acids used for antibacterial include fumaric acid, succinic acid, L-valine, malic acid, citric acid, glycine ( Glycine) and hippuric acid (Hippuric acid). Its ratio is consistent with the content in pangolin scales. Among them, in the total extract (CV), fumaric acid accounts for 0.1%, succinic acid accounts for 0.4%, L-valine accounts for 1.3%, and malic acid accounts for 1.2%. %, citric acid accounted for 0.2%, glycine accounted for 0.1% and hippuric acid accounted for 0.3%. Preferably, the above ratio is adjustable and effective.

The microorganisms involved in this paper can include Gram-negative bacteria, Gram-positive bacteria, or include Staphylococcus aureus as the representative of pathogenic Staphylococcus, Escherichia coli as the representative of conditional pathogenic bacteria or other pathogenic bacteria, Such as Mycobacterium tuberculosis.

The organic acids used in the experiments and composition components of the present invention are all synthetic organic acids.

Through the disparate content ratio of such compositions, a better antibacterial effect can be achieved compared with a single or less composition of the same volume.

Example 1

Antibacterial tests were carried out based on the minimum inhibitory concentration method (MIC), and the above antibacterial metabolites were used for Escherichia coli (E. The minimum inhibitory concentration (MIC; Minimum Inhibitory Concentration) detection of P.aeruginosa; Pseudomonas aeruginosa) and Serratia marcescens (S.marcescens; Serratiamarcescens).

(1) Experimental materials:

1) culture medium

Lysogeny Broth (LB; Lysogeny Broth) medium containing 10 g/L peptone, 5 g/L yeast extract and 10 g/L sodium chloride was used.

2) Antibacterial drugs:

The whole composition comprises one or more of fumaric acid, succinic acid, L-valine, malic acid, citric acid, glycine and hippuric acid.

(2) The steps of antibacterial test are as follows:

1) Preparation of bacterial suspension

The preserved Escherichia coli representing Gram-negative bacteria and Staphylococcus aureus representing Gram-positive bacteria were resuscitated simultaneously. Use an inoculation loop to pick a single colony with a diameter of about 1mm on the plate of the purified bacteria to be tested, inoculate it in 2mL LB broth, and culture it in a biochemical incubator at 37°C. After enrichment, use LB broth to correct the concentration of the bacteria solution in the logarithmic growth phase to 0.5 McFarland turbidimetric standard (that is, the absorbance at 625nm is 0.08-0.10), containing about 1×10 ⁸ to 2×10 ⁸ CFU·mL ^-1 .

2) Preparation of antibacterial drugs

Prepare combined antibacterial drugs with concentrations of 4C, 2C, C, 0.5C, 0.25C, 0.125C, 0.0625C, and 0.03125C by double dilution method, take 8 sterile test tubes and number them in sequence, and add them to 2-8 test tubes 1ml sterile water. Prepare 2ml of 4C antibacterial drug in the first test tube, take 1ml of the solution in the first test tube and add it to the second test tube, after mixing, take 1ml of the solution in the second test tube and add it to the third test tube, and then dilution.

3) MIC determination

Add 200 μL of LB broth medium to column 1 of the 96-well plate as a negative control, and add 180 μL of prepared bacterial suspension to column 2-10 to a final concentration of 1×10 ⁵ CFU·mL ^-1 . Add 20 μL of sterile water to column 2 as a positive control group, and add 20 μL of drug concentration to columns 3 to 10. The final concentrations are 0.4, 0.2, 0.1, 0.05, 0.025, 0.0125, 0.00625 and 0.003125C. The respectively inoculated drug-sensitivity plates were placed in a 37°C incubator and incubated for 24 hours. The MIC value was detected by observing the turbidity of the LB broth medium and detecting the OD value. The experiment was repeated 3 times.

The results obtained are shown in Table 1 as the minimum inhibitory concentration of each metabolite to Escherichia coli and Staphylococcus aureus, and the summary of independent concentrated antibacterial metabolites. The best inhibitory effect on Escherichia coli and Staphylococcus aureus is malic acid.

Table 1

In order to verify the optimal effect of malic acid and its composition, this experiment further used malic acid and other metabolites as a composition to conduct antibacterial tests, and the results represented by MIC values are shown in Table 2.

Table 2

The experimental results showed that the MIC value of the two-component composition added with malic acid on Escherichia coli and Staphylococcus aureus became smaller, indicating that it had a higher inhibitory effect on Escherichia coli and Staphylococcus aureus. Further, the random distribution of the three compositions was carried out, and the antibacterial test was carried out, as shown in Table 3.

table 3

The combination of malic acid and fumaric acid has weak antibacterial effect when combined with L-valine or citric acid. Similarly, malic acid and L-valine have the same results when combined with glycine or hippuric acid , the inhibitory effect on Escherichia coli and Staphylococcus aureus weakened, and the MIC value rose back to 0.4C. The combination of malic acid, hippuric acid and citric acid has the best inhibitory effect on Escherichia coli, with an MIC value of 0.1C.

In this experiment, malic acid was selected as the main component, and four compositions were formed by assigning other metabolites and malic acid to conduct antibacterial tests. The results are shown in Table 4.

Table 4

The results showed that the composition of malic acid, fumaric acid, glycine and hippuric acid and the composition of malic acid, citric acid, glycine and hippuric acid can form the antibacterial effect with MIC as low as 0.1C.

In this experiment, malic acid was selected as the main component, and five compositions composed of other metabolites and malic acid were selected for antibacterial test. The results are shown in Table 5.

table 5

The results showed that malic acid, L-valine, citric acid, glycine, hippuric acid; malic acid, fumaric acid, succinic acid, L-valine, hippuric acid; malic acid, fumaric acid, L-valine The three compositions of acid, glycine and hippuric acid can form a bacteriostatic effect on Escherichia coli with an MIC value of 0.1C. And the composition malic acid, succinic acid, L-valine, glycine, hippuric acid; malic acid, fumaric acid, L-valine, citric acid, hippuric acid; malic acid, fumaric acid, citric acid, glycine , hippuric acid; malic acid, succinic acid, L-valine, citric acid, hippuric acid; any group of malic acid, succinic acid, citric acid, glycine, hippuric acid can form MIC against Escherichia coli and Staphylococcus aureus Bacteriostatic effect with a value of 0.1C. Compared with the use of a single organic acid or two organic acids as additives in the prior art, its antibacterial effect is not as good as the antibacterial additive formed by combining more than four organic acids.

In this experiment, malic acid was selected as the main component, and six compositions and seven metabolites were mixed by assigning other metabolites and malic acid to conduct antibacterial tests. The results are shown in Table 6.

Table 6

The results showed that even if six or more metabolites were mixed, their antibacterial effect on Staphylococcus aureus and Escherichia coli could reach MIC value of 0.1C.

Further, taking Escherichia coli, Staphylococcus metallobacter, Pseudomonas aeruginosa and Serratia marcescens as examples, the metabolite combination containing malic acid, hippuric acid, fumaric acid and glycine was tested, and the results are shown in Table 7:

Table 7

The results showed that the organic acid metabolite composition composed of malic acid, hippuric acid, fumaric acid and glycine had an antibacterial effect on Escherichia coli and Staphylococcus aureus reaching an MIC value of 0.1C, The antibacterial effect of bacteria reached MIC value 0.2C. The organic acid metabolite composition composed of malic acid, hippuric acid, fumaric acid and glycine has antibacterial properties against bacteria represented by Escherichia coli, Staphylococcus aureus, Pseudomonas aeruginosa and Serratia marcescens, especially pathogenic bacteria inhibition of its growth.

Serratia marcescens is the smallest bacterium, a Gram-negative bacillus, and an opportunistic pathogen.

Pseudomonas aeruginosa is a gram-negative bacterium that is an opportunistic infection.

Escherichia coli, Staphylococcus aureus, Pseudomonas aeruginosa and Serratia marcescens are widely distributed in nature. They can enter the human body through the mouth and nose of the human body, and cause inflammation and other pathogenic reactions in the human body under certain conditions or environmental conditions. Therefore, in this experiment, the above four strains were selected as the test objects of the antibacterial test.

Based on the above experimental results, the composition composed of malic acid, glycine and hippuric acid can be combined with citric acid or fumaric acid to form an antibacterial effect on Staphylococcus aureus and Escherichia coli with an MIC value of 0.1C.

The proportions in the composition used in the experiment above can be 0.1% for fumaric acid, 0.4% for succinic acid, 1.3% for L-valine, 1.2% for malic acid, and 0.2% for citric acid. %, glycine accounted for 0.1% and hippuric acid accounted for 0.3%, and the antibacterial composition was prepared in the above proportions.

Preferably, the composition for reducing the reproduction tendency of microorganisms existing on the surface of the human body or in the environment where the human body is located comprises: 0.125%-0.482% malic acid, 0.135%-0.422% L-valine, 0.021%-0.081% One or more of citric acid, 0.01%-0.04% succinic acid, 0.031%-0.121% hippuric acid, 0.001%-0.041% glycine and 0.01%-0.041% fumaric acid.

Preferably, the ratio of each component in the composition can be: malic acid accounts for 1% to 30% of the total components, L-valine accounts for 0.1% to 20% of the total components, citric acid Accounting for 0.1%-20% of the total components, 0.1%-20% for succinic acid, 0.1%-20% for hippuric acid, and 0.1%-20% for glycine The ratio is 0.1% to 20% and fumaric acid accounts for 0.1% to 20% of the total components.

Preferably, the ratio of each component in the composition can be: malic acid accounts for 10% to 40% of the total components, L-valine accounts for 0.01% to 10% of the total components, citric acid Accounting for 0.01%-10% of the total components, 0.01%-20% for succinic acid, 0.01%-20% for hippuric acid, and 0.01%-20% for glycine The ratio is 0.01% to 20% and fumaric acid accounts for 0.01% to 10% of the total components.

Preferably, the malic acid can range from about 0.1% to about 20%, about 1% to about 10%, about 3% to about 8%, about 6% to about 12%, about In the range of 15% to about 18%, about 17% to about 20%, 20% to 60%, or by weight about 1%, about 2%, about 3%, about 4%, about 5%, about 6%, about 7%, about 8%, about 9%, about 10%, about 15%, about 20%, about 30%, or about 40%, including all ranges and subranges therebetween.

Preferably, the L-valine can range from about 0.01% to about 1%, from about 1% to about 10%, from about 3% to about 8%, from about 6% to about 12% by weight in the total components. %, about 15% to about 18%, about 17% to about 20%, or about 1%, about 2%, about 3%, about 4%, about 5%, about 6% by weight, About 7%, about 8%, about 9%, about 10%, about 15%, about 20%, about 30%, or about 40%, including all ranges and subranges therebetween.

Preferably, the citric acid can range from about 0.01% to about 2%, about 1% to about 10%, about 3% to about 8%, about 6% to about 12%, about In the range of 15% to about 18%, about 17% to about 20%, or about 1%, about 2%, about 3%, about 4%, about 5%, about 6%, about 7% by weight , about 8%, about 9%, about 10%, about 15%, about 20%, about 30%, or about 40%, including all ranges and subranges therebetween.

Preferably, the succinic acid can range from about 0.1% to about 20%, about 1% to about 10%, about 3% to about 8%, about 6% to about 12%, about In the range of 15% to about 18%, about 17% to about 20%, or about 1%, about 2%, about 3%, about 4%, about 5%, about 6%, about 7% by weight , about 8%, about 9%, about 10%, about 15%, about 20%, about 30%, or about 40%, including all ranges and subranges therebetween.

Preferably, the hippuric acid can comprise from about 0.1% to about 20%, about 1% to about 10%, about 3% to about 8%, about 6% to about 12%, about In the range of 15% to about 18%, about 17% to about 20%, 20% to 60%, or by weight about 1%, about 2%, about 3%, about 4%, about 5%, about 6%, about 7%, about 8%, about 9%, about 10%, about 15%, about 20%, about 30%, or about 40%, including all ranges and subranges therebetween.

Preferably, the glycine can range from about 0.1% to about 2%, about 1% to about 10%, about 3% to about 8%, about 6% to about 12%, about 15% by weight of the total components % to about 18%, about 17% to about 20%, or by weight about 1%, about 2%, about 3%, about 4%, about 5%, about 6%, about 7%, About 8%, about 9%, about 10%, about 15%, about 20%, about 30%, or about 40%, including all ranges and subranges therebetween.

Preferably, the fumaric acid can range from about 0.01% to about 20%, about 1% to about 10%, about 3% to about 8%, about 6% to about 12%, In the range of about 15% to about 18%, about 17% to about 20%, or about 1%, about 2%, about 3%, about 4%, about 5%, about 6%, about 7% by weight %, about 8%, about 9%, about 10%, about 15%, about 20%, about 30%, or about 40%, including all ranges and subranges therebetween.

Preferably, the combination range of malic acid, L-valine, citric acid, succinic acid, hippuric acid, glycine and fumaric acid can include but not limited to the above ratio and combination range.

The composition with MIC value reaching 0.1C for Escherichia coli is represented by the composition of malic acid, hippuric acid and citric acid, while the composition with MIC value reaching 0.1C for Escherichia coli and Staphylococcus aureus at the same time The composition is represented by malic acid, fumaric acid, glycine, hippuric acid; malic acid, citric acid, glycine, hippuric acid. Since the above-mentioned components forming the composition are all weakly acidic components and can be absorbed by the human body as food additives, the above-mentioned multi-group composition can be used in various fields as components that are harmless to the human body. In the prior art, the method and cognition of using organic acid is: any organic acid has antibacterial effect, and it is added in large doses within the scope permitted by law to antibacterial That's it. However, when organic acids are used as additives in large quantities, conversion products will be produced in the body. Small doses rely on metabolism, but large doses cannot guarantee that all products can be metabolized out of the body in a timely manner. bodily harm. The present invention obtains the optimal bacteriostatic composition through the bacteriostatic ratio of effective bacteriostatic organic acids, and can reduce the amount of edible organic acids by increasing the types of edible organic acids, thereby helping food or other While ensuring the antiseptic effect, it will have a healthier impact on the human body.

Example 2

This embodiment is a further improvement on Embodiment 1, and repeated content will not be repeated here.

Take the Liyan Tablet currently used in the treatment of chronic pharyngitis as an example. For chronic pharyngitis caused by bacterial infection, Liyan Tablets can have a better effect on the disease. The ingredients of Liyan Tablets include pangolin (manufactured), wood beetle, silkworm, oyster (calcined), and scrophulariaceae. The pangolin (made) in the ingredients is taken from endangered organisms. This ingredient is a banned drug, so Liyan Tablets cannot be used. Treat patients as prescription or over-the-counter medicines. The present invention explores the inhibitory effect on bacterial flora in pangolin scales, especially Escherichia coli and Staphylococcus aureus, and finds one or more groups of compositions that have effective inhibitory effects on Escherichia coli and Staphylococcus aureus. The main components of the medicine include: one or more of malic acid, hippuric acid, fumaric acid, glycine, succinic acid, L-valine and citric acid. When replacing pangolin ingredients in Liyan Tablets, on the one hand, the replacement ingredients can use biological tissue extracts with similar ingredients, and on the other hand, the replacement ingredients can also be artificially synthesized effective antibacterial metabolite substitute ingredients. Preferably, when the synthetic compound is used as the replacement component, it can have the best inhibitory effect on Gram-negative bacteria represented by Escherichia coli and Gram-positive bacteria represented by Staphylococcus aureus designed in this experiment One of the compositions: malic acid, fumaric acid, succinic acid, L-valine, citric acid and glycine. The addition of the above-mentioned organic acid can also bring fruity and other flavors popular to children to the tablet, thereby increasing the enthusiasm of infants and young children to take medicine.

Example 3

This embodiment is a further improvement on Embodiment 1 or Embodiment 2, and repeated content will not be repeated here.

The composition is also used in various foods as an additive composition to effectively inhibit Escherichia coli and Staphylococcus aureus in foods. For example, adding it to some natural fruit juices increases the natural flavor through the ratio of malic acid and citric acid, and cooperates with the fruit juice in vacuum or sealed packaging to achieve antiseptic effect by inhibiting the growth of Escherichia coli and Staphylococcus aureus. The main components of the additive include: one or more of malic acid, hippuric acid, fumaric acid, glycine, succinic acid, L-valine and citric acid. Malic acid and citric acid alone or in combination form an additive with a fruity flavor.

Example 4

This embodiment is a further improvement on Embodiment 1, Embodiment 2 or Embodiment 3, and repeated content will not be repeated.

The composition can also be used in households or to disinfect bacteria under normal living conditions. For example, in home life, it is necessary to maintain antibacterial bacteria in the environment or clean clothes antibacterially, especially Staphylococcus aureus, which is harmful to the human body. The disinfection of alcohol or 84 disinfectant is also harmful to the human body, and it is not suitable for families with pregnant women or children. The present invention can form a liquid reagent for disinfecting bacteria suitable for families with children or pregnant women based on the composition of the weakly acidic component and other harmless bacteria-killing components.

Compositions composed of organic acids can be used as additives in detergents or bactericides. The composition as an additive is used for antibacterial and deodorizing of clothes or environment. The composition is provided with a first composition as a pH regulator and a second composition for increasing the disinfecting effect and time. The first composition comprises any one of two combinations: malic acid, glycine, hippuric acid, citric acid; malic acid, glycine, hippuric acid, fumaric acid. The second composition comprises a hydrocolloid.

Hydrocolloids comprising polysaccharides and proteins form a gel network. When the Escherichia coli or Staphylococcus aureus carried by the target of detergent or bactericide application is wrapped by the gel network, the first composition present in the hydrocolloid enters the bacterial cell from the cell based on the pressure of the gel network on the cell. Inside the cell, the first composition and the second composition work together to form a synergistic organic acid, release H ⁺ inside the cell, destroy the alkaline environment inside the cell, and thereby inhibit the activity of the cell. The first composition is introduced into the hydrocolloid, and acts on the inside of the Escherichia coli or the Staphylococcus aureus along with the encapsulation of the Escherichia coli or the Staphylococcus aureus carried by the target of the application of the detergent or bactericide by the hydrocolloid.

The molecular diameter of the organic acid used to act on the bacterial intracellular environment can be smaller than the diameter of the cell membrane channel of Escherichia coli and Staphylococcus aureus so that the organic acid molecule can enter the inside of the cell through the cell membrane channel and destroy the alkalinity inside the cell The environment inhibits bacterial cell activity.

Through the synergistic effect of the first composition composed of malic acid, glycine and hippuric acid and the second composition composed of citric acid or fumaric acid, the co-inhibitory effect of Escherichia coli and Staphylococcus aureus is realized.

Example 5

This embodiment is a further improvement on Embodiment 1, Embodiment 2, Embodiment 3 or Embodiment 4, and repeated content will not be repeated.

The present invention designs a composition, which is used to spread to the target surface by spraying, atomizing or other means of forming small particle gas or liquid, and disinfecting and inhibiting Escherichia coli and Staphylococcus aureus on the target surface.

Compositions consist of combined liquid or gaseous particles. The above-mentioned liquid or gas particles contain a particle core composed of an efficient antibacterial organic acid and a nano-scale inorganic microporous zirconia-tin oxide composite covering the particle core that is neutralized by a silane coupling agent for protection and protection. Outer mantle for increased adhesion.

The nano-scale inorganic microporous zirconia-tin oxide composite that is neutralized by the silane coupling agent that wraps the organic acid is dispersed in the clothes and living environment in the form of aerosol, aerosol or small particle liquid. When the zirconia-tin oxide complex in nanoscale inorganic microporous form, which is neutralized by the silane coupling agent of the acid, touches Escherichia coli and Staphylococcus aureus on the target surface, the outer film releases organic acids based on pressure and allows the organic acids to pass through the bacteria The cell membrane on the surface enters the bacterial body, thereby inhibiting bacterial activity. The nanoscale inorganic micropores formed by the zirconia-tin oxide composite can allow organic acid molecules to enter and exit, and limit the hindrance of the decomposition and attachment of large-volume particles to the particles. The zirconia-tin oxide composite can effectively maintain the hardness of the outer coating and the toughness of preventing nano-micropore collapse when forming the outer coating of particles in the form of aerosol, liquid or gas.

Preferably, the ratio of the silane coupling agent to the nanoscale inorganic microporous zirconia-tin oxide composite is 1:40-1:120. The ratio of nanoscale inorganic microporous zirconia-tin oxide composite to organic acid is between 1:1 and 1:10.

Microparticles composed of organic-inorganic bodies coupled with silane coupling agents can move to the target surface with a diameter of at least less than 10 μm. The above-mentioned microparticles have excellent dispersion stability in the water phase for dilute or homogeneous liquid phase, high adhesion rate of carrying organic acid on the target surface, and high volatility performance of reducing the core of organic acid due to the wrapping of the outer film. Using a low-cost mesophase that can stably couple the internal organic layer and the external inorganic layer, increase the inorganic layer that is coupled with the organic layer and does not undergo other chemical reactions without damaging the ionization of hydrogen ions in the organic layer , The properties of the inorganic layer overcome the problems of poor adhesion, no effect and volatile nature of the organic layer on the target surface. Preferably, the coupling with a silane coupling agent can be coupling with an aqueous silane coupling agent.

The preparation method of above-mentioned composition is as follows:

(1) Weighing: Weigh the silane coupling agent coupling and zirconia tin oxide complex (which can exist in the form of sol) in weight conversion according to the proportion, and weigh the organic acid composition in volume conversion;

(2) Mixing: Put the zirconia tin oxide complex into the organic acid in batches, mix well, then slowly pour into the silane coupling agent for coupling with the stirring action, and stir until the three phases are uniform, that is, the concentrated combination is obtained thing.

The concentrated composition can be diluted with water before use, and then sprayed on the target surface requiring disinfection such as the environment or clothing through equipment such as an aerosol machine and a watering can.

The organic acid at the core of the above particles can be combined with the organic acid composition mentioned in the above examples that can have an bacteriostatic effect on Escherichia coli and Staphylococcus aureus with an MIC of 0.1C. The combination of organic acids covered by the outer film involved in the present invention has good stability in water, and has high-efficiency inhibitory effect on Escherichia coli and Staphylococcus aureus.

Example 6

This embodiment is a further improvement on Embodiment 1, Embodiment 2, Embodiment 3, Embodiment 4 or Embodiment 5, and repeated content will not be repeated.

For the above-mentioned organic acid composition that can be permanently attached to the surface and act on bacteria, the present invention can design a composition containing one of malic acid, hippuric acid, fumaric acid, glycine, succinic acid, L-valine and citric acid. One or more antibacterial ointments or sprays, which can act on the skin surface or mucous membranes such as the face, arms, and vital organs. The organic acid composition has obvious antibacterial effect on the reproduction of various pathogenic skin bacteria such as Escherichia coli and Staphylococcus aureus as representatives of Gram-negative bacteria and Gram-positive bacteria.

Preferably, the antibacterial ointment includes traditional Chinese medicine ointment, traditional Chinese medicine powder, mussel mucin and one or more of malic acid, hippuric acid, fumaric acid, glycine, succinic acid, L-valine and citric acid. Several organic acid compositions.

Preferably, the antibacterial spray contains pentylene glycol, 1,3 propylene glycol and one or more of malic acid, hippuric acid, fumaric acid, glycine, succinic acid, L-valine and citric acid organic acid composition.

It should be noted that the above specific embodiments are exemplary, and those skilled in the art can come up with various solutions inspired by the disclosure of the present invention, and these solutions also belong to the scope of the disclosure of the present invention and fall within the scope of this disclosure. within the scope of protection of the invention. Those skilled in the art should understand that the description and drawings of the present invention are illustrative rather than limiting to the claims. The protection scope of the present invention is defined by the claims and their equivalents. The description of the present invention contains a number of inventive concepts, such as "preferably", "according to a preferred embodiment" or "optionally" all indicate that the corresponding paragraph discloses an independent concept, and the applicant reserves the right to propose a division based on each inventive concept right to apply. Throughout the text, the features introduced by "preferably" are only optional, and should not be interpreted as having to be set. Therefore, the applicant reserves the right to waive or delete relevant preferred features at any time.

Claims (9)

1. A composition for spraying onto a human body surface or onto the environment in which the human body is located, comprising: 1.2 percent of malic acid, 0.3 percent of hippuric acid, 0.2 percent of citric acid, 0.1 percent of glycine and the balance of water, and the composition cleans a human body or the environment where the human body is positioned in a mode of inhibiting the propagation of escherichia coli and staphylococcus aureus.

2. The composition of claim 1, wherein the composition further comprises: the mass percent of the L-valine is 1.3 percent.

3. The composition according to claim 1 or 2, characterized in that it further comprises: the mass percent is 0.4 percent of succinic acid.

4. Use of the composition according to claim 1 for the preparation of a detergent, an antibacterial drug, a bactericide or a food additive.

5. A composition, comprising: 0.1 percent of fumaric acid, 1.2 percent of malic acid, 0.3 percent of hippuric acid, 0.1 percent of glycine and the balance of water.

6. The composition of claim 5, wherein the composition further comprises: 1.3% L-valine.

7. The composition according to claim 5 or 6, characterized in that it further comprises: 0.2% citric acid.

8. Use of a composition according to claim 5 in the preparation of an oral cleanser.

9. Use of the composition according to claim 5 for the preparation of a detergent, an antibacterial drug, a bactericide or a food additive.