CN113980791A - Automatic microorganism inoculation instrument and inoculation scribing method thereof - Google Patents
Automatic microorganism inoculation instrument and inoculation scribing method thereof Download PDFInfo
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- CN113980791A CN113980791A CN202111448260.1A CN202111448260A CN113980791A CN 113980791 A CN113980791 A CN 113980791A CN 202111448260 A CN202111448260 A CN 202111448260A CN 113980791 A CN113980791 A CN 113980791A
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- 238000011081 inoculation Methods 0.000 title claims abstract description 64
- 244000005700 microbiome Species 0.000 title claims abstract description 39
- 238000000034 method Methods 0.000 title claims description 10
- 239000012528 membrane Substances 0.000 claims abstract description 24
- XEEYBQQBJWHFJM-UHFFFAOYSA-N Iron Chemical compound [Fe] XEEYBQQBJWHFJM-UHFFFAOYSA-N 0.000 claims description 12
- 240000007643 Phytolacca americana Species 0.000 claims description 8
- 229910052742 iron Inorganic materials 0.000 claims description 6
- 239000000463 material Substances 0.000 claims description 6
- 230000000149 penetrating effect Effects 0.000 claims description 3
- 238000001179 sorption measurement Methods 0.000 claims description 3
- 238000009434 installation Methods 0.000 description 5
- 238000010586 diagram Methods 0.000 description 2
- 238000012986 modification Methods 0.000 description 2
- 230000004048 modification Effects 0.000 description 2
- 206010063659 Aversion Diseases 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 230000007547 defect Effects 0.000 description 1
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M33/00—Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus
- C12M33/02—Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus by impregnation, e.g. using swabs or loops
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
- C12M41/48—Automatic or computerized control
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- Apparatus Associated With Microorganisms And Enzymes (AREA)
Abstract
The invention relates to an automatic microorganism inoculation instrument, which comprises a mobile robot and a scribing component; the scribing assembly comprises an inoculation rod, a driving unit and a positioning unit; the driving unit comprises two transverse elastic membranes, a mounting frame and an X Y-axis moving unit, and the inoculation rod vertically penetrates through the two elastic membranes; the positioning unit comprises two combined plates and a transverse moving unit; the surfaces of the opposite sides of the two combined plates are respectively provided with a first copying groove and a second copying groove, and the first copying groove and the second copying groove are spliced to form a continuous S-shaped positioning groove when the two combined plates are spliced; a positioning ring is sleeved and fixed on the inoculation rod, and a positioning groove matched with the positioning ring is arranged on the inner wall of the positioning groove; not only can promote precision and uniformity by a wide margin, simultaneously can very simple and easy change compoboard form different continuous S type shapes, can customize, overall structure is reasonable and compact, and the cost is lower.
Description
Technical Field
The invention relates to the technical field of microorganism inoculators, in particular to an automatic microorganism inoculator and an inoculation scribing method thereof.
Background
The microorganism inoculation instrument is used for inoculating microorganisms and is a common microorganism culture instrument; at present, during scribing operation, most of the scribing operations are manual operation or simple machine-driven moving operation, the stability of the scribing operation is very poor, the consistency is almost zero, and the precision of the scribing operation is still not ideal although certain stability and consistency can be guaranteed.
Disclosure of Invention
The technical problem to be solved by the present invention is to provide an automatic microorganism inoculator and an inoculation streaking method thereof, aiming at the above-mentioned defects of the prior art.
The technical scheme adopted by the invention for solving the technical problems is as follows:
constructing an automatic microorganism inoculation instrument, wherein the automatic microorganism inoculation instrument comprises a mobile robot and a scribing component arranged at the movable end of the mobile robot; the scribing assembly comprises an inoculation rod, a driving unit for driving the inoculation rod to move and a positioning unit for movably positioning the inoculation rod; the driving unit comprises two transverse elastic membranes which are longitudinally arranged side by side, a mounting frame for mounting the two elastic membranes and an X Y-axis moving unit for driving the mounting frame to move along an X-axis and a Y-axis, and the inoculation rod vertically penetrates through the two elastic membranes and is fixedly connected with the two elastic membranes at a penetrating position; the positioning unit comprises two combined plates and a transverse moving unit which drives the two combined plates to transversely move for combination; the surfaces of the opposite sides of the two combined plates are respectively provided with a first profiling groove and a second profiling groove, and when the two combined plates are spliced, the first profiling groove and the second profiling groove are spliced to form a continuous S-shaped positioning groove; the inoculating rod is sleeved and fixed with a positioning ring, and a positioning groove matched with the positioning ring is formed in the inner wall of the positioning groove.
The automatic microorganism inoculation instrument comprises a transverse moving unit, wherein the transverse moving unit comprises a clamping cylinder, the clamping cylinder is installed on an installation frame, a positioning channel for positioning two combined plates is arranged on the installation frame, and a first poking rod and a second poking rod extending into the positioning channel are respectively arranged at two movable ends of the clamping cylinder; the positioning channel is provided with an opening for the inoculation rod to pass through.
The automatic microorganism inoculation instrument provided by the invention is characterized in that the first poke rod and the second poke rod are both iron pieces, and the combined plate is provided with an adsorption magnet of the iron pieces.
The automatic microorganism inoculation instrument provided by the invention is characterized in that one side of the positioning channel, which is far away from the clamping cylinder, is provided with an opening for a combined plate to enter and exit the positioning channel.
The invention relates to an automatic microorganism inoculation instrument, which further comprises a combined plate storage unit and a combined plate material moving manipulator; the combined board storage unit is used for storing combined boards, and the combined board moving manipulator is used for moving the combined boards between the combined board storage unit and the positioning channel.
The automatic microorganism inoculation instrument further comprises a control host, and the mobile robot, the scribing component and the combined plate material moving manipulator are electrically connected with and controlled by the control host.
The invention relates to an automatic microorganism inoculation instrument, wherein one surface of one side, opposite to the two combined plates, of each combined plate is provided with a conical positioning lug, and the other surface of each combined plate is provided with a conical positioning groove.
The inoculation scribing method of the automatic microorganism inoculator is applied to the automatic microorganism inoculator and comprises the following steps:
selecting a required continuous S-shaped scribing shape and correspondingly selecting two combined plates to be loaded into the movable end of the transverse moving unit;
the transverse moving unit moves and splices the two combined plates, the spliced inoculation rod is positioned in the positioning groove, and the positioning ring on the inoculation rod is positioned in the positioning groove;
the driving unit is driven to move by the mobile robot, so that the lower end of the inoculation rod reaches the starting point of the marking position;
the X Y axis moving unit operates to drive the two transverse elastic membranes to move along the X axis and the Y axis, and the two elastic membranes drive the inoculation rod to move along the positioning groove for marking.
The invention has the beneficial effects that: by applying the method, the required continuous S-shaped marking shape is selected, two combined plates are correspondingly selected to be arranged at the movable end of the transverse moving unit, the transverse moving unit moves to split the two combined plates, the split inoculating rod is positioned in the positioning groove, and the positioning ring on the inoculating rod is positioned in the positioning groove; the mobile robot drives the driving unit to move, so that the lower end of the inoculating rod reaches the starting point of the marking position, the X Y-axis moving unit operates to drive the two transverse elastic membranes to move along the X axis and the Y axis, and the two elastic membranes drive the inoculating rod to move along the positioning groove for marking; not only can promote precision and uniformity by a wide margin, simultaneously can very simple and easy change compoboard form different continuous S type shapes, can customize, overall structure is reasonable and compact, and the cost is lower.
Drawings
In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the present invention will be further described with reference to the accompanying drawings and embodiments, wherein the drawings in the following description are only part of the embodiments of the present invention, and for those skilled in the art, other drawings can be obtained without inventive efforts according to the accompanying drawings:
FIG. 1 is a schematic view of an inoculation rod driving structure of an automatic microorganism inoculator according to a preferred embodiment of the present invention;
FIG. 2 is a schematic view of a combination of two boards of an automatic microorganism inoculator according to a preferred embodiment of the present invention;
FIG. 3 is a schematic diagram of the traverse unit of the automatic microorganism inoculator according to the preferred embodiment of the present invention;
FIG. 4 is a flow chart of the streaking method in the automatic microorganism inoculator according to the preferred embodiment of the present invention.
Detailed Description
In order to make the objects, technical solutions and advantages of the embodiments of the present invention clearer, the following will clearly and completely describe the technical solutions in the embodiments of the present invention, and it is obvious that the described embodiments are some embodiments of the present invention, but not all embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments of the present invention without inventive step, are within the scope of the present invention.
The automatic microorganism inoculator in the preferred embodiment of the invention, as shown in fig. 1, and referring to fig. 2 and 3, comprises a mobile robot and a marking assembly arranged at the movable end thereof; the marking assembly comprises an inoculation rod 1, a driving unit for driving the inoculation rod 1 to move and a positioning unit for movably positioning the inoculation rod; the driving unit comprises two transverse elastic membranes 2 which are longitudinally arranged side by side, a mounting rack 3 for mounting the two elastic membranes 2 and an X Y-axis moving unit 4 for driving the mounting rack 3 to move along an X-axis and a Y-axis, and the inoculation rod 1 vertically penetrates through the two elastic membranes 2 and is fixedly connected with the two elastic membranes 2 at the penetrating positions; the positioning unit comprises two combined plates 5 and a transverse moving unit for driving the two combined plates 5 to transversely move for combination; the surfaces of the opposite sides of the two combined plates 5 are respectively provided with a first contour groove 50 and a second contour groove 51, and the first contour groove 50 and the second contour groove 51 are spliced to form a continuous S-shaped positioning groove 52 when the two combined plates 5 are spliced; a positioning ring 10 is sleeved and fixed on the inoculation rod 1, and a positioning groove 53 matched with the positioning ring is arranged on the inner wall of the positioning groove 52;
by applying the method, the required continuous S-shaped marking shape is selected, two combined plates 5 are correspondingly selected to be arranged at the movable end of the transverse moving unit, the transverse moving unit moves and splices the two combined plates 5, the spliced inoculation rod 1 is positioned in the positioning groove 52, and the positioning ring 10 on the inoculation rod 1 is positioned in the positioning groove 53; the mobile robot drives the driving unit to move, so that the lower end of the inoculation rod 1 reaches the starting point of the marking position, the X Y-axis moving unit 4 operates to drive the two transverse elastic membranes 2 to move along the X axis and the Y axis, and the two elastic membranes 2 drive the inoculation rod 1 to move along the positioning groove 52 for marking;
the precision and the consistency can be greatly improved, and meanwhile, the combined plate 5 can be simply replaced to form different continuous S-shaped shapes, so that the customized combined plate can be customized, the integral structure is reasonable and compact, and the cost is lower;
it should be noted that the X Y-axis moving unit shown in fig. 1 is only a schematic diagram, and an existing X Y-axis moving device is used in actual installation;
preferably, the transverse moving unit comprises a clamping cylinder 6, the clamping cylinder 6 is installed on the installation frame 3, a positioning channel 7 for positioning the two combined plates is arranged on the installation frame 3, and a first poke rod 60 and a second poke rod 61 extending into the positioning channel 7 are respectively arranged at two movable ends of the clamping cylinder 6; the positioning channel is provided with an opening through which the inoculation rod passes; the wholeness is good, is difficult for appearing the aversion condition, and the drive structure is very succinct simultaneously, and the cost is lower.
Preferably, the first poke rod 60 and the second poke rod 61 are both iron pieces, and the combination plate 5 is provided with an adsorption magnet of the iron pieces; the combined plate is convenient to drive to move and reset, and meanwhile, the combined plate is convenient to assemble and disassemble.
Preferably, an opening 70 for the combination board 5 to go in and out of the positioning channel is arranged on one side of the positioning channel 7, which is far away from the clamping cylinder 6; reasonable layout and convenient assembly and disassembly of the combined board.
Preferably, the automatic microorganism inoculation instrument further comprises a combined plate storage unit and a combined plate material moving manipulator; the combined plate storage unit is used for storing combined plates, and the combined plate material moving manipulator is used for moving the combined plates between the combined plate storage unit and the positioning channel; the automatic microorganism inoculation instrument also comprises a control host, and the mobile robot, the scribing component and the combined plate material moving manipulator are all electrically connected with and controlled by the control host; the automatic operation control is convenient, and meanwhile, the assembly plate can be automatically assembled and disassembled through the design mode;
preferably, one of the two combined plates 5 is provided with a conical positioning lug, and the other combined plate 5 is provided with a conical positioning groove; the combination is convenient to carry out accurate contraposition.
An inoculation streaking method for an automatic microorganism inoculator is applied to the automatic microorganism inoculator, and is implemented as follows as shown in fig. 4:
s01: selecting a required continuous S-shaped scribing shape and correspondingly selecting two combined plates to be loaded into the movable end of the transverse moving unit;
s02: the transverse moving unit moves and splices the two combined plates, the spliced inoculation rod is positioned in the positioning groove, and the positioning ring on the inoculation rod is positioned in the positioning groove;
s03: the driving unit is driven to move by the mobile robot, so that the lower end of the inoculation rod reaches the starting point of the marking position;
s04: x Y axis moving unit operates to drive two transverse elastic membranes to move along X axis and Y axis, and two elastic membranes drive the inoculation rod to move along the positioning groove for marking;
not only can promote precision and uniformity by a wide margin, simultaneously can very simple and easy change compoboard form different continuous S type shapes, can customize, overall structure is reasonable and compact, and the cost is lower.
It will be understood that modifications and variations can be made by persons skilled in the art in light of the above teachings and all such modifications and variations are intended to be included within the scope of the invention as defined in the appended claims.
Claims (8)
1. An automatic microorganism inoculation instrument is characterized by comprising a mobile robot and a scribing component arranged at the movable end of the mobile robot; the scribing assembly comprises an inoculation rod, a driving unit for driving the inoculation rod to move and a positioning unit for movably positioning the inoculation rod; the driving unit comprises two transverse elastic membranes which are longitudinally arranged side by side, a mounting frame for mounting the two elastic membranes and an X Y-axis moving unit for driving the mounting frame to move along an X-axis and a Y-axis, and the inoculation rod vertically penetrates through the two elastic membranes and is fixedly connected with the two elastic membranes at a penetrating position; the positioning unit comprises two combined plates and a transverse moving unit which drives the two combined plates to transversely move for combination; the surfaces of the opposite sides of the two combined plates are respectively provided with a first profiling groove and a second profiling groove, and when the two combined plates are spliced, the first profiling groove and the second profiling groove are spliced to form a continuous S-shaped positioning groove; the inoculating rod is sleeved and fixed with a positioning ring, and a positioning groove matched with the positioning ring is formed in the inner wall of the positioning groove.
2. The automatic microorganism inoculator according to claim 1, wherein the traverse unit comprises a clamping cylinder, the clamping cylinder is mounted on the mounting frame, a positioning channel for positioning the two combination boards is arranged on the mounting frame, and a first poking rod and a second poking rod extending into the positioning channels are respectively arranged at two movable ends of the clamping cylinder; the positioning channel is provided with an opening for the inoculation rod to pass through.
3. The automatic microorganism inoculation instrument according to claim 2, wherein the first poke rod and the second poke rod are both iron pieces, and the combination plate is provided with an adsorption magnet of the iron pieces.
4. The automatic microorganism inoculation instrument according to claim 2, wherein the side of the positioning channel facing away from the clamping cylinder is provided with an opening for the combination board to enter and exit the positioning channel.
5. The automatic microorganism inoculation instrument according to claim 4, wherein the automatic microorganism inoculation instrument further comprises a composition plate storage unit and a composition plate transferring manipulator; the combined board storage unit is used for storing combined boards, and the combined board moving manipulator is used for moving the combined boards between the combined board storage unit and the positioning channel.
6. The automatic microorganism inoculation instrument of claim 5, wherein the automatic microorganism inoculation instrument further comprises a control host, and the mobile robot, the streaking assembly and the composition plate material transferring manipulator are all electrically connected with and controlled by the control host.
7. The automatic microorganism inoculator according to any one of claims 1-6, wherein the two combined plates are provided with a conical positioning projection on one surface and a conical positioning groove on the other surface on the opposite side.
8. An inoculation streaking method for an automatic microorganism inoculator, which is applied to the automatic microorganism inoculator as claimed in any one of claims 1 to 7, and is characterized by comprising the following steps:
selecting a required continuous S-shaped scribing shape and correspondingly selecting two combined plates to be loaded into the movable end of the transverse moving unit;
the transverse moving unit moves and splices the two combined plates, the spliced inoculation rod is positioned in the positioning groove, and the positioning ring on the inoculation rod is positioned in the positioning groove;
the driving unit is driven to move by the mobile robot, so that the lower end of the inoculation rod reaches the starting point of the marking position;
the X Y axis moving unit operates to drive the two transverse elastic membranes to move along the X axis and the Y axis, and the two elastic membranes drive the inoculation rod to move along the positioning groove for marking.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202111448260.1A CN113980791B (en) | 2021-11-30 | 2021-11-30 | Automatic microorganism inoculation instrument and inoculation scribing method thereof |
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| Application Number | Priority Date | Filing Date | Title |
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| CN202111448260.1A CN113980791B (en) | 2021-11-30 | 2021-11-30 | Automatic microorganism inoculation instrument and inoculation scribing method thereof |
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| Publication Number | Publication Date |
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| CN113980791A true CN113980791A (en) | 2022-01-28 |
| CN113980791B CN113980791B (en) | 2024-01-12 |
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Citations (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US3830701A (en) * | 1970-09-08 | 1974-08-20 | Hycel Inc | Automatic petri dish streaking methods and apparatus |
| US3935075A (en) * | 1974-05-13 | 1976-01-27 | Diagnostic Research, Inc. | Automatic bacterial specimen streaker and method for using same |
| US4635369A (en) * | 1986-04-29 | 1987-01-13 | Powell Tommie L | Scribing device and method |
| CN206562431U (en) * | 2017-01-22 | 2017-10-17 | 皖西学院 | A kind of microorganism seperation culture device |
| CN107513491A (en) * | 2017-09-29 | 2017-12-26 | 河北工程大学 | Streak inoculation device |
| CN108587885A (en) * | 2018-04-23 | 2018-09-28 | 山东省农业科学院农业资源与环境研究所 | A kind of micro-biological samples streak inoculation device and method |
| CN208995497U (en) * | 2018-10-17 | 2019-06-18 | 宁波卫生职业技术学院 | An inoculation device that can automatically partition and scribe a petri dish |
| CN112143633A (en) * | 2020-10-13 | 2020-12-29 | 上海莫杜生物科技有限公司 | Semi-automatic microbial inoculation appearance |
| CN112662523A (en) * | 2021-02-08 | 2021-04-16 | 济南百博生物技术股份有限公司 | Automatic scribing device and scribing method for microbial sample pretreatment system |
-
2021
- 2021-11-30 CN CN202111448260.1A patent/CN113980791B/en active Active
Patent Citations (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US3830701A (en) * | 1970-09-08 | 1974-08-20 | Hycel Inc | Automatic petri dish streaking methods and apparatus |
| US3935075A (en) * | 1974-05-13 | 1976-01-27 | Diagnostic Research, Inc. | Automatic bacterial specimen streaker and method for using same |
| US4635369A (en) * | 1986-04-29 | 1987-01-13 | Powell Tommie L | Scribing device and method |
| CN206562431U (en) * | 2017-01-22 | 2017-10-17 | 皖西学院 | A kind of microorganism seperation culture device |
| CN107513491A (en) * | 2017-09-29 | 2017-12-26 | 河北工程大学 | Streak inoculation device |
| CN108587885A (en) * | 2018-04-23 | 2018-09-28 | 山东省农业科学院农业资源与环境研究所 | A kind of micro-biological samples streak inoculation device and method |
| CN208995497U (en) * | 2018-10-17 | 2019-06-18 | 宁波卫生职业技术学院 | An inoculation device that can automatically partition and scribe a petri dish |
| CN112143633A (en) * | 2020-10-13 | 2020-12-29 | 上海莫杜生物科技有限公司 | Semi-automatic microbial inoculation appearance |
| CN112662523A (en) * | 2021-02-08 | 2021-04-16 | 济南百博生物技术股份有限公司 | Automatic scribing device and scribing method for microbial sample pretreatment system |
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