CN113975342A - A traditional Chinese medicine compound for improving immunity of chickens - Google Patents
A traditional Chinese medicine compound for improving immunity of chickens Download PDFInfo
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Abstract
本发明名称为一种提高鸡群免疫的中药复合物。本发明提供的中药复合物本方中，是属于纯天然的保健食品。由洋葱、金银花、五味子，上述三种纯天然食品相辅相成，经过煮沸、过滤、蒸馏，从而具有提高免疫力的作用。实验表明，本发明中药复合物能够提高淋巴细胞的增殖，以及调节淋巴细胞因子IFN‑γ、IL‑2和IL‑4的分泌，从而起到提高免疫力的作用。本文首次研究了中药复合物提高免疫力的作用，为进一步发展天然功能性食品提供了基础。The name of the invention is a traditional Chinese medicine compound for improving immunity of chickens. The traditional Chinese medicine compound provided by the present invention is a pure natural health food. By onion, honeysuckle, schisandra, the above three kinds of pure natural food complement each other, after boiling, filtering and distillation, it has the effect of improving immunity. Experiments show that the traditional Chinese medicine compound of the present invention can improve the proliferation of lymphocytes and regulate the secretion of lymphocyte factors IFN-γ, IL-2 and IL-4, thereby improving immunity. This paper is the first to study the effect of traditional Chinese medicine complexes in improving immunity, which provides a basis for the further development of natural functional foods.
Description
Technical Field
The invention discloses a pure natural food for enhancing immunity, belonging to the technical field of food health care.
Background
Poultry infectious diseases are the largest factors threatening poultry breeding industry, the most main immunization measure adopted by the poultry breeding industry at present is to add antibiotics into feed, however, the antibiotics may generate certain drug resistance, and factors such as large drug residue, high immunization cost and the like cause Chinese and foreign scholars to change the research direction, and the search for natural food with low toxicity and no residue becomes the main research direction at present.
IL-2 is an important regulator of cell function and is responsible for a variety of immunomodulatory and immunostimulatory tasks, such as T cell activation, triggering of natural killer cells, inflammation, and autoimmune diseases. Maintenance of regulatory T cells and CD4 by stimulation of IL-2+It is crucial that T cells differentiate into specific effector T cell subsets following antigen-mediated activation. For CD8+T cells, IL-2 signaling, can optimize T cell production and differentiation effects into memory cells. IL-2, in soluble form or in combination with dendritic cells and extracellular matrix, IL-2 alone or in combination with specific neutralizing IL-2 specific antibodies, can amplify CD8+T cells respond to or induce expansion of T cell populations, thereby facilitating immune stimulation or suppression. IL-4 is a cytokine produced primarily by activated T lymphocytes, particularly TH2 cells, mast cells and basophils. IL-4 is a key cytokine that assists TH2 cellular immune response, and IL-4 plays a role in humoral responses, inducing B cell switching to subtypes such as IgE and IgG. IFN-gamma is a key product of TH1 cells, an important mediator of immunity and inflammation, and it activates STAT1 transcription factor using JAK-STAT signal pathway.
The onion is a perennial herb of Allium of Liliaceae, and contains a large amount of nutrients such as carbohydrate, protein and water soluble vitamins, especially the outer skin of the onion contains a large amount of quercetin and quercetin derivatives, and the onion quercetin has pharmacological effects of resisting oxidation, inflammation, obesity, bacteria, cancer and brain.
Honeysuckle is a dried flower bud or a flower with initial blossom of a plant of the same genus and the plant of the genus Lonicera of the family Caprifoliaceae and is known as a good medicine for clearing heat and removing toxicity from ancient times. It is sweet and cold in nature and fragrant, sweet and cold in nature and clearing heat without hurting stomach, and the fragrance is thorough and can eliminate pathogens. The honeysuckle can disperse wind-heat and clear away blood toxin, and has obvious effect on various heat diseases such as fever, eruption, macula, sore and carbuncle due to heat toxin, sore throat and the like.
Fructus Schisandrae is dried mature fruit of Schisandra chinensis of Magnoliaceae. The ancient medical book called him 33614c, mysterious union 34127, mysterious union, confluence, the earliest listed in the Shennong herbal meridian top-grade traditional Chinese medicines can nourish and strengthen the strength, has extremely high medicinal value and the effect of strengthening the body, and is used together with Qiongzhen lucid ganoderma to treat insomnia.
The research selects the three pure natural foods, develops the traditional Chinese medicine compound for improving the immunity of the chicken flocks, has stable efficacy and no residue, and is suitable for long-term administration.
Disclosure of Invention
The invention aims to develop a traditional Chinese medicine compound for improving the immunity of chicken flocks.
The purpose of the invention is realized by the following technical scheme:
a Chinese medicinal composition for improving chicken immunity is prepared from Bulbus Allii Cepae, flos Lonicerae, and fructus Schisandrae.
Further limited, the following components are prepared according to the weight portion: 20-50 parts of onion, 10-30 parts of honeysuckle and 15-40 parts of schisandra chinensis.
Further defined, the components are preferably: 30-40 parts of onion, 15-25 parts of honeysuckle and 25-35 parts of schisandra chinensis.
Further limiting, soaking 8 times of distilled water in total weight of the raw materials for one hour, then decocting for 1 time, decocting for 4 hours each time, combining, filtering as soon as possible, removing filter residues, and concentrating the filtrate to a proper volume to prepare the traditional Chinese medicine compound for improving the immunity of the chicken flocks.
Further, the formulation of the traditional Chinese medicine compound for improving chicken immunity can be decoction oral liquid.
The above natural foods are commercially available.
The invention has the beneficial effects that: a Chinese medicinal composition for improving immunity of chicken is effective in promoting proliferation of chicken peripheral blood lymphocyte lymph, promoting secretion of cytokine IL-2 and IFN-gamma, and inhibiting secretion of cytokine IL-4, and has natural and residue-free effects.
Drawings
FIG. 1 is a diagram showing the effect of a Chinese medicinal composition for enhancing immunity of chicken flocks, promoting the proliferation of peripheral blood lymphocytes and regulating the secretion of peripheral blood lymphokines.
Detailed Description
In order that those skilled in the art will better understand the technical solution and practice the invention, the following further describes the invention with reference to specific examples.
A traditional Chinese medicine compound for improving chicken flock immunity is prepared from the following components in parts by weight: 30-40 parts of onion, 15-25 parts of honeysuckle and 25-35 parts of schisandra chinensis. Soaking 8 times of distilled water in parts by weight of the raw materials for one hour, then decocting for 1 time, decocting for 4 hours each time, mixing, filtering as soon as possible, removing filter residues, and concentrating the filtrate to a proper volume to obtain the traditional Chinese medicine compound for improving the immunity of chicken flocks.
Example 1
A traditional Chinese medicine compound for improving chicken flock immunity is prepared from the following components in parts by weight: 30 parts of onion, 15 parts of honeysuckle and 25 parts of schisandra chinensis. The Chinese medicinal composition is prepared by the above method.
Example 2
A traditional Chinese medicine compound for improving chicken flock immunity is prepared from the following components in parts by weight: 35 parts of onion, 20 parts of honeysuckle and 30 parts of schisandra chinensis. The Chinese medicinal composition is prepared by the above method.
Example 3
A traditional Chinese medicine compound for improving chicken flock immunity is prepared from the following components in parts by weight: 40 parts of onion, 25 parts of honeysuckle and 35 parts of schisandra chinensis. The Chinese medicinal composition is prepared by the above method.
The influence of the traditional Chinese medicine compound on the immunity is shown in the following experiments:
the first experiment method comprises the following steps:
1. laboratory animals and groups
300 avine broilers of 1 day old are adopted in the method, and are randomly divided into 5 groups of 20 broilers.
Blank, positive, example 1, example 2, and example 3 groups, respectively. The blank group was gavaged with basal diet for 35 days. The positive group was gavaged with levamisole (100 mg/kg. bw) for 35 days. Examples 1, 2, and 3 groups were gavaged with varying doses of herbal composition (300mg/kg · bw) for 35 days.
2. Preparation of peripheral blood lymphocyte suspension
Randomly drawing 10 broilers in each group, taking 3mL of offshoot venous blood of the broilers in an aseptic mode, adopting heparin for anticoagulation, transferring the anticoagulated blood into a centrifugal tube after autoclaving, drawing 1mL of whole blood, transferring the whole blood into a 4mL EP tube, and adding the same volume of the whole blood and tissue diluent; adding 3ml of separating medium, flatly spreading the diluted whole blood above the separating medium, keeping two liquid levels clear, centrifuging for 25min at 1500r/min at room temperature, dividing the tube into four layers after centrifugation, and respectively: a serum layer, a lymphocyte leucocyte layer, a separation liquid layer and a red blood cell layer. The leukocyte membrane layer was carefully pipetted into a 15mL centrifuge tube and the lymphocytes were washed with 10mL of PBS buffer or cell wash. Centrifuging at 4 deg.C and 500r/min for 10min, discarding supernatant, and resuspending cells in 5ml cell washing solution. Centrifuging at 4 deg.C for 10min at 500r/min, and removing supernatant; 4mL of complete culture medium is added into a 15mL centrifugal tube and evenly blown to prepare lymphocyte suspension for later use.
3. Proliferation of lymphocytes
Using a 96-well plate, 100. mu.L of lymphocyte suspension per well, 3 replicates per group, were placed in a cell culture chamber at 37 ℃ with 5% CO2Culturing for 48h, adding 20 μ L MTT solution into each well, culturing for 4h, discarding supernatant, adding 150 μ L DMSO, and measuring OD value with microplate reader at 570 nm.
4. Secretion of lymphokines
Using 24-well cell culture plates, 1mL of lymphocyte suspension was added to each well at 37 ℃ with 5% CO2Culturing for 48 h. Detecting the effect of the Chinese medicinal composition on the secretion of IFN-gamma, IL-2 and IL-4 of peripheral blood lymphocytes of chicken by ELISA methodKit instructions were run and 3 replicates were set up for each group.
II, experimental results:
1. effect of Chinese medicinal composition on lymphocyte proliferation
As shown in table 1, the positive, example 1, example 2 and example 3 groups were compared to the blank group: the positive group, the example 1 group, the example 2 group and the example 3 group are all very significant (P <0.01) higher than the blank group, and the traditional Chinese medicine compound can promote the proliferation of peripheral blood lymphocytes of the broilers.
TABLE 1 Effect of herbal complexes on lymphocyte proliferation
| Group of | OD value |
| Blank group | 1.01±0.04 |
| Positive group | 2.10±0.08** |
| Example 1 group | 1.62±0.06** |
| Example 2 group | 1.63±0.06** |
| Example 3 group | 2.05±0.04** |
Note: in table 1, P <0.05, P <0.01 compared to the normal group.
2. Effect of Chinese medicinal composition on secretion of lymphocyte cytokine
As can be seen from table 2, the positive, example 1, example 2 and example 3 groups were compared to the blank group: the positive group, the example 1 group, the example 2 group and the example 3 group are all very significant (P <0.01), and the traditional Chinese medicine compound can regulate the secretion of broiler chicken peripheral blood lymphokine IFN-gamma, IL-2 and IL-4.
TABLE 2 Effect of herbal complexes on lymphocyte cytokine secretion
| Group of | IFN-γ | IL-2 | IL-4 |
| Blank group | 1.19±0.03 | 0.86±0.07 | 0.77±0.07 |
| Positive group | 2.22±0.03** | 1.89±0.03** | 1.93±0.14** |
| Example 1 group | 1.46±0.02** | 1.34±0.04** | 1.26±0.08** |
| Example 2 group | 1.65±0.09** | 1.58±0.06** | 1.41±0.09** |
| Example 3 group | 1.73±0.04** | 1.76±0.04** | 1.57±0.05** |
Note: in table 2, P <0.05, P <0.01 compared to the normal group.
Claims (4)
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