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CN113974039B - Noni fruit ferment free of peculiar smell and preparation method thereof - Google Patents

Noni fruit ferment free of peculiar smell and preparation method thereof Download PDF

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Publication number
CN113974039B
CN113974039B CN202111328768.8A CN202111328768A CN113974039B CN 113974039 B CN113974039 B CN 113974039B CN 202111328768 A CN202111328768 A CN 202111328768A CN 113974039 B CN113974039 B CN 113974039B
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noni
noni fruit
vanilla
odorless
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CN113974039A (en
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李小宝
张斌
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Hainan Mulada Biotechnology Co.,Ltd.
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Hainan Normal University
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    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23LFOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES, NOT OTHERWISE PROVIDED FOR; PREPARATION OR TREATMENT THEREOF
    • A23L2/00Non-alcoholic beverages; Dry compositions or concentrates therefor; Preparation or treatment thereof
    • A23L2/38Other non-alcoholic beverages
    • A23L2/382Other non-alcoholic beverages fermented
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23LFOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES, NOT OTHERWISE PROVIDED FOR; PREPARATION OR TREATMENT THEREOF
    • A23L2/00Non-alcoholic beverages; Dry compositions or concentrates therefor; Preparation or treatment thereof
    • A23L2/52Adding ingredients
    • A23L2/56Flavouring or bittering agents
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23LFOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES, NOT OTHERWISE PROVIDED FOR; PREPARATION OR TREATMENT THEREOF
    • A23L2/00Non-alcoholic beverages; Dry compositions or concentrates therefor; Preparation or treatment thereof
    • A23L2/52Adding ingredients
    • A23L2/60Sweeteners
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23LFOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES, NOT OTHERWISE PROVIDED FOR; PREPARATION OR TREATMENT THEREOF
    • A23L2/00Non-alcoholic beverages; Dry compositions or concentrates therefor; Preparation or treatment thereof
    • A23L2/70Clarifying or fining of non-alcoholic beverages; Removing unwanted matter
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23LFOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES, NOT OTHERWISE PROVIDED FOR; PREPARATION OR TREATMENT THEREOF
    • A23L2/00Non-alcoholic beverages; Dry compositions or concentrates therefor; Preparation or treatment thereof
    • A23L2/70Clarifying or fining of non-alcoholic beverages; Removing unwanted matter
    • A23L2/80Clarifying or fining of non-alcoholic beverages; Removing unwanted matter by adsorption
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23LFOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES, NOT OTHERWISE PROVIDED FOR; PREPARATION OR TREATMENT THEREOF
    • A23L33/00Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23VINDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
    • A23V2002/00Food compositions, function of food ingredients or processes for food or foodstuffs
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23VINDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
    • A23V2400/00Lactic or propionic acid bacteria
    • A23V2400/11Lactobacillus
    • A23V2400/123Bulgaricus
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23VINDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
    • A23V2400/00Lactic or propionic acid bacteria
    • A23V2400/11Lactobacillus
    • A23V2400/175Rhamnosus
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23VINDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
    • A23V2400/00Lactic or propionic acid bacteria
    • A23V2400/21Streptococcus, lactococcus
    • A23V2400/231Lactis
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23VINDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
    • A23V2400/00Lactic or propionic acid bacteria
    • A23V2400/21Streptococcus, lactococcus
    • A23V2400/249Thermophilus

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  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Nutrition Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Food Science & Technology (AREA)
  • Polymers & Plastics (AREA)
  • Mycology (AREA)
  • Coloring Foods And Improving Nutritive Qualities (AREA)
  • Preparation Of Fruits And Vegetables (AREA)

Abstract

The application provides noni enzyme free of peculiar smell, which comprises, by weight, 100 parts of noni, 5-10 parts of shredded coconut, 1-10 parts of vanilla, 5-10 parts of additives and 50-300 parts of distilled water, wherein the vanilla is a pod, basil, rosemary and peppermint. After the noni fruit ferment without peculiar smell disclosed by the application is used for processing noni fruits, the noni fruit ferment is fermented with shredded coconut, vanilla pod, basil, rosemary and peppermint in a compound way, and finally, the noni fruit ferment is subjected to active carbon adsorption treatment, so that the peculiar smell in the noni fruits can be effectively reduced, and the palatability of the noni fruit ferment is improved.

Description

一种无异味的诺丽果酵素及其制备方法A kind of noni fruit enzyme without peculiar smell and its preparation method

技术领域Technical field

本发明涉及食品领域,特别涉及一种无异味的诺丽果酵素及其制备方法。The invention relates to the field of food, and in particular to an odorless noni enzyme and a preparation method thereof.

背景技术Background technique

诺丽果,又名海巴戟,是茜草科巴戟天属植物。高1-5米,茎直,枝近四棱柱形;叶交互对生,长圆形、椭圆形或卵圆形,长12-25厘米,两端渐尖或急尖,通常具光泽,无毛,全缘;叶脉两面凸起,托叶生叶柄间,每侧1枚,上部扩大呈半圆形,全缘,无毛。生于海滨平地或疏林下,主要生长在南太平洋群岛,中国海南岛、西沙群岛和台湾岛有分布。Noni, also known as Morinda citrifolia, is a plant of the genus Morinda of the Rubiaceae family. 1-5 meters high, straight stems, nearly quadrangular branches; leaves alternately opposite, oblong, oval or oval, 12-25 cm long, both ends acuminate or acute, usually glossy, without Hairy, entire margin; leaf veins are convex on both sides, stipules are between the petioles, 1 on each side, the upper part is enlarged in a semicircular shape, entire margin, glabrous. It grows on coastal plains or under sparse forests. It mainly grows in the South Pacific Islands, and is distributed in Hainan Island, Xisha Islands and Taiwan Island in China.

现代医学研究以证实,它含有275种以上的营养成分。其中包括14种维生素、16种矿物质,以及人体所需的多种氨基酸,抗氧化物,还含有东莨菪碱,多醣体等,还有罕见的对人体健康非常重要的17种虹甙类。所以,诺丽具有非常强效的天然活性营养和预防及治疗医学价值。Modern medical research has confirmed that it contains more than 275 kinds of nutrients. It includes 14 kinds of vitamins, 16 kinds of minerals, as well as a variety of amino acids and antioxidants needed by the human body. It also contains scopolamine, polysaccharides, etc., as well as 17 rare kinds of iridosides that are very important to human health. Therefore, Noni has very powerful natural active nutrition and preventive and therapeutic medical value.

诺丽可以生食或熟食,既可采取黄色成熟的果实,日晒后阴藏一日,采收果实汁液,也可直接榨取新鲜果汁食用。在南太平洋地区,人们采摘下成熟的白色诺丽果,清洗干净后放到一个密闭容器中,果实开始软化,并经过一定时间进行发酵,获得的纯液体即为原液或酵素,可作为天然药物服用。此外,成熟的诺丽果还具有一股特殊的丁酸异味,鲜食或者打成汁味道很差,不易被人们接受。随着现代加工技术和生物技术的发展,目前市场上的诺丽产品有诺丽果发酵液、诺丽酵素片、诺丽果酒、诺丽果饮料等。Noni can be eaten raw or cooked. You can take the yellow ripe fruit, store it in the shade for a day after being exposed to the sun, and harvest the fruit juice. You can also squeeze the fresh juice directly for consumption. In the South Pacific, people pick ripe white noni fruits, clean them and put them in a closed container. The fruits begin to soften and ferment after a certain period of time. The pure liquid obtained is the original solution or enzyme, which can be used as a natural medicine. Take. In addition, ripe noni fruit also has a special butyric acid smell. It tastes very bad when eaten fresh or made into juice, and is not easily accepted by people. With the development of modern processing technology and biotechnology, Noni products currently on the market include Noni fruit fermentation liquid, Noni enzyme tablets, Noni fruit wine, Noni fruit beverage, etc.

专利CN110122803A公开了一种诺丽果片及其制备方法。包括以下重量份数的组分:诺丽干果片100份、混合药材提取液30~200份;其中,所述混合药材提取液由以下重量份数的组分组成:五指毛桃2~5份、薏苡仁2~5份、百合1~4份、黑果枸杞0.5~3份、玉竹0.5~3份、决明子0.1~2份、荷叶0.1~2份和山柰0.1~2份;按配比,将混合药材提取液均匀喷淋到诺丽干果片上,烘干至诺丽果片含水量低于5%,即得。该专利采用了大量的中药材对诺丽果进行处理,会影响诺丽果的口味,产生中药味。且没有解决诺丽果酵素的异味问题。Patent CN110122803A discloses a noni fruit slice and its preparation method. It includes the following components by weight: 100 parts of dried noni fruit slices, 30-200 parts of mixed medicinal material extract; wherein, the mixed medicinal material extract consists of the following components by weight: 2-5 parts of Wuzhi Maotao, 2 to 5 parts of coix seed, 1 to 4 parts of lily, 0.5 to 3 parts of black wolfberry, 0.5 to 3 parts of Polygonatum odorifera, 0.1 to 2 parts of cassia, 0.1 to 2 parts of lotus leaf, and 0.1 to 2 parts of kaempferol; according to the ratio , spray the mixed medicinal material extract evenly onto the dried Noni fruit slices, and dry them until the moisture content of the Noni slices is less than 5%. This patent uses a large number of Chinese medicinal materials to process the Noni fruit, which will affect the taste of the Noni fruit and produce a Chinese medicinal taste. And it does not solve the odor problem of noni fruit enzyme.

专利CN110093239A公开了一种诺丽配制酒及其制备方法,同样采用了中药材复配解决诺丽果的异味问题。Patent CN110093239A discloses a noni-prepared wine and its preparation method, which also uses a compound of traditional Chinese medicinal materials to solve the problem of the peculiar smell of noni fruit.

目前世面上对诺丽果产品的除臭均采用中药草复配处理,会导致最终产品中药味中;且均以茶、酒、果干、蛋糕等形式出现,目前未见诺丽果常见食用方法酵素类产品的除臭方法。At present, the deodorization of noni products in the world uses Chinese herbal medicine compound processing, which will cause the final product to have a Chinese herbal taste; and all of them appear in the form of tea, wine, dried fruits, cakes, etc. Currently, there is no common consumption of noni fruit. Methods for deodorizing enzyme products.

发明内容Contents of the invention

鉴以此,本发明的目的在于提出一种无异味的诺丽果酵素及其制备方法。In view of this, the object of the present invention is to propose a noni enzyme without peculiar smell and its preparation method.

一种无异味的诺丽果酵素,由以下重量份的组分组成:诺丽果100份,椰蓉5-10份,香草1-10份,添加剂2-10份和蒸馏水50-300份。An odorless noni enzyme is composed of the following components by weight: 100 parts of noni, 5-10 parts of coconut, 1-10 parts of vanilla, 2-10 parts of additives and 50-300 parts of distilled water.

优选的,所述香草为香草荚、罗勒、迷迭香和薄荷。Preferably, the herbs are vanilla pod, basil, rosemary and mint.

优选的,所述添加剂为稳定剂0.01-0.5份,维生素C 0.1-1份,余量为甜味剂。Preferably, the additives are 0.01-0.5 parts of stabilizer, 0.1-1 part of vitamin C, and the balance is sweetener.

优选的,所述甜味剂为白砂糖、葡萄糖、D-山梨糖醇、甜菊糖苷中一种或多种的混合物。Preferably, the sweetener is a mixture of one or more of white sugar, glucose, D-sorbitol, and steviol glycosides.

优选的,一种无异味的诺丽果酵素,由以下重量份的组分组成:诺丽果100份,椰蓉7份,香草荚4份,罗勒2份,迷迭香1份,薄荷0.5份,白砂糖2份,D-山梨糖醇1份,甜菊糖苷0.1份,稳定剂0.05份,维生素C 0.5份和蒸馏水150份。Preferably, an odorless noni enzyme is composed of the following components by weight: 100 parts of noni, 7 parts of coconut, 4 parts of vanilla pods, 2 parts of basil, 1 part of rosemary, and 0.5 part of mint. , 2 parts of white sugar, 1 part of D-sorbitol, 0.1 part of stevia glycoside, 0.05 part of stabilizer, 0.5 part of vitamin C and 150 parts of distilled water.

一种无异味的诺丽果酵素及其制备方法,包括以下步骤:An odorless noni fruit enzyme and its preparation method include the following steps:

步骤1:筛选诺丽果鲜果,选取充分成熟、汁液丰富的诺丽果,用臭氧消毒杀菌后,再用清水清洗、切片,沥干水分,放入乙醇-水混合溶液,浸泡;Step 1: Screen the fresh noni fruits, select fully mature and juicy noni fruits, disinfect and sterilize them with ozone, then wash them with clean water, slice them, drain them, put them into an ethanol-water mixed solution, and soak them;

步骤2:将步骤1中的诺丽果片取出,冷冻干燥后,经破壁粉碎,加入椰蓉、香草,以及蒸馏水,搅拌混合;Step 2: Take out the noni fruit slices in step 1, freeze-dry them, crush them, add coconut mash, vanilla, and distilled water, stir and mix;

步骤3:在步骤2所得混合物中,加入菌种,发酵;Step 3: Add bacteria to the mixture obtained in step 2 and ferment;

步骤4:将步骤3所得混合物灭菌,过滤,用含有活性炭的载体进行吸附处理;Step 4: Sterilize the mixture obtained in Step 3, filter it, and use a carrier containing activated carbon for adsorption treatment;

步骤5:加入添加剂,调节口味,再次灭菌灭酶,灌装、消毒,得到一种无异味的诺丽果酵素。Step 5: Add additives to adjust the taste, sterilize and sterilize the enzyme again, fill and sterilize, and obtain an odorless noni enzyme.

优选的,所述步骤1中,臭氧的浓度为15-20ppm;乙醇-水混合溶液中,乙醇的浓度为60-75%wt,浸泡时间为1-2h,浸泡温度为10-20℃。Preferably, in step 1, the concentration of ozone is 15-20ppm; in the ethanol-water mixed solution, the concentration of ethanol is 60-75%wt, the soaking time is 1-2h, and the soaking temperature is 10-20°C.

优选的,所述步骤3中,所述菌种为保加利亚乳杆菌、鼠李糖乳杆菌和乳酸链球菌,接种量为诺丽果混合物重量的0.3-0.5%。Preferably, in step 3, the bacterial species are Lactobacillus bulgaricus, Lactobacillus rhamnosus and Streptococcus lactis, and the inoculum amount is 0.3-0.5% of the weight of the noni fruit mixture.

优选的,保加利亚乳杆菌、鼠李糖乳杆菌和乳酸链球菌的用量比为10:5-8:3-5。Preferably, the dosage ratio of Lactobacillus bulgaricus, Lactobacillus rhamnosus and Streptococcus lactis is 10:5-8:3-5.

优选的,所述步骤3中,发酵温度为15-30℃,发酵时间为25-50D。Preferably, in step 3, the fermentation temperature is 15-30°C and the fermentation time is 25-50D.

与现有技术相比,本发明的有益效果为:Compared with the prior art, the beneficial effects of the present invention are:

1)本申请仅采用了椰蓉和香草与诺丽果混合发酵,且添加量较少,保留了诺丽果独特的口味;1) This application only uses coconut milk, vanilla and noni fruit for mixed fermentation, and the addition amount is small, retaining the unique taste of noni fruit;

2)本申请对诺丽果鲜果采用乙醇-水浸泡处理,去除了诺丽果中大部分异味物质,降低了诺丽果中的异味物质;2) This application uses ethanol-water soaking treatment for fresh noni fruit, which removes most of the odor substances in the noni fruit and reduces the odor substances in the noni fruit;

3)本申请采用活性炭吸附,去除了部分诺丽果中的异味物质;3) This application uses activated carbon adsorption to remove some of the odor substances in the noni fruit;

4)本申请采用椰蓉、香草与诺丽果复配发酵,不仅能进一步降低诺丽果酵素中的异味,还可以丰富诺丽果酵素的香味,增加适口性;4) This application uses the compound fermentation of coconut, vanilla and noni fruit, which can not only further reduce the odor in the noni fruit enzyme, but also enrich the fragrance of the noni fruit enzyme and increase the palatability;

5)本申请采用了多种甜味剂,所得诺丽果酵素中含糖量较低,是一种低糖饮料。5) This application uses a variety of sweeteners, and the obtained noni fruit enzyme has a low sugar content, making it a low-sugar drink.

具体实施方式Detailed ways

为对本发明中的技术方案进行清楚、完整地描述,显然,发明人结合实施例进行说明,但以下实施例所描述的仅仅是本发明一部分实施例,而不是全部的实施例。基于本发明中的实施例,本领域普通技术人员在没有做出创造性劳动前提下所获得的所有其他实施例,都属于本发明保护的范围。In order to clearly and completely describe the technical solutions in the present invention, obviously, the inventors illustrate in conjunction with examples. However, the following examples describe only some of the embodiments of the present invention, rather than all the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by those of ordinary skill in the art without creative efforts fall within the scope of protection of the present invention.

一种无异味的诺丽果酵素,由以下重量份的组分组成:诺丽果100份,椰蓉5-10份,香草1-10份,添加剂5-10份和蒸馏水50-300份;所述香草为香草荚、罗勒、迷迭香和薄荷;所述添加剂为稳定剂0.01-0.5份,维生素C 0.1-1份,余量为甜味剂;所述甜味剂为白砂糖、葡萄糖、D-山梨糖醇、甜菊糖苷中一种或多种的混合物。An odorless noni enzyme is composed of the following components by weight: 100 parts of noni, 5-10 parts of coconut, 1-10 parts of vanilla, 5-10 parts of additives and 50-300 parts of distilled water; The vanilla is vanilla pod, basil, rosemary and mint; the additive is 0.01-0.5 part of stabilizer, 0.1-1 part of vitamin C, and the balance is sweetener; the sweetener is white sugar, glucose, A mixture of one or more of D-sorbitol and steviol glycosides.

实施例1:Example 1:

选取诺丽果100份,椰蓉5份,香草荚2份,罗勒2份,迷迭香2份,薄荷0.5份,稳定剂0.01份,维生素C1份,甜味剂4份,蒸馏水100份。Select 100 parts of noni, 5 parts of coconut, 2 parts of vanilla pod, 2 parts of basil, 2 parts of rosemary, 0.5 part of mint, 0.01 part of stabilizer, 1 part of vitamin C, 4 parts of sweetener, and 100 parts of distilled water.

甜味剂为葡萄糖1.95份、D-山梨糖醇2份、甜菊糖苷0.05份。The sweeteners are 1.95 parts of glucose, 2 parts of D-sorbitol, and 0.05 parts of steviol glycosides.

制备方法:Preparation:

步骤1:筛选诺丽果鲜果,选取充分成熟、汁液丰富的诺丽果,用15ppm臭氧消毒杀菌后,再用清水清洗、切片,沥干水分,放入70%wt乙醇-水混合溶液,浸泡,浸泡时间为1.5h,浸泡温度为10℃;Step 1: Screen the fresh noni fruits, select fully mature and juicy noni fruits, disinfect and sterilize with 15ppm ozone, then wash and slice with clean water, drain the water, put in 70% wt ethanol-water mixed solution, and soak , soaking time is 1.5h, soaking temperature is 10℃;

步骤2:将步骤1中的诺丽果片取出,冷冻干燥后,经破壁粉碎,加入椰蓉、香草,以及蒸馏水,搅拌混合;Step 2: Take out the noni fruit slices in step 1, freeze-dry them, crush them, add coconut mash, vanilla, and distilled water, stir and mix;

步骤3:在步骤2所得混合物中,加入菌种,发酵;所述菌种为保加利亚乳杆菌、鼠李糖乳杆菌和乳酸链球菌,比例为10:6:4,接种量为诺丽果混合物重量的0.4%,发酵温度为25℃,发酵时间为40D。Step 3: Add bacterial strains to the mixture obtained in step 2 and ferment; the bacterial strains are Lactobacillus bulgaricus, Lactobacillus rhamnosus and Streptococcus lactis, the ratio is 10:6:4, and the inoculum amount is the mixture of noni fruits 0.4% by weight, the fermentation temperature is 25°C, and the fermentation time is 40D.

步骤4:将步骤3所得混合物灭菌,过滤,用含有活性炭的载体进行吸附处理;Step 4: Sterilize the mixture obtained in Step 3, filter it, and use a carrier containing activated carbon for adsorption treatment;

步骤5:加入添加剂,调节口味,再次灭菌灭酶,灌装、消毒,得到一种无异味的诺丽果酵素。Step 5: Add additives to adjust the taste, sterilize and sterilize the enzyme again, fill and sterilize, and obtain an odorless noni enzyme.

实施例2:Example 2:

选取诺丽果100份,椰蓉8份,香草荚1份,罗勒2份,迷迭香4份,薄荷1份,稳定剂0.2份,维生素C 0.5份,甜味剂2份,蒸馏水200份;Select 100 parts of noni, 8 parts of coconut, 1 part of vanilla pod, 2 parts of basil, 4 parts of rosemary, 1 part of mint, 0.2 parts of stabilizer, 0.5 parts of vitamin C, 2 parts of sweetener, and 200 parts of distilled water;

甜味剂为白砂糖1.85份、甜菊糖苷0.15份。The sweetener is 1.85 parts of white sugar and 0.15 parts of steviol glycosides.

其他参数与制备方法和实施例1相同。Other parameters are the same as the preparation method and Example 1.

实施例3:Example 3:

选取诺丽果100份,椰蓉7份,香草荚4份,罗勒2份,迷迭香1份,薄荷0.5份,稳定剂0.05份,维生素C 0.5份,甜味剂3.1份,蒸馏水150份;Select 100 parts of noni, 7 parts of coconut, 4 parts of vanilla pod, 2 parts of basil, 1 part of rosemary, 0.5 part of mint, 0.05 part of stabilizer, 0.5 part of vitamin C, 3.1 parts of sweetener, and 150 parts of distilled water;

甜味剂为白砂糖2份、D-山梨糖醇1份、甜菊糖苷0.1份。The sweeteners are 2 parts of white sugar, 1 part of D-sorbitol, and 0.1 part of steviol glycosides.

其他参数与制备方法和实施例1相同。Other parameters are the same as the preparation method and Example 1.

实施例4:Example 4:

选取诺丽果100份,椰蓉10份,香草荚4份,罗勒2份,迷迭香1份,薄荷0.5份,稳定剂0.5份,维生素C 0.1份,甜味剂4份,蒸馏水300份;Select 100 parts of noni, 10 parts of coconut, 4 parts of vanilla pod, 2 parts of basil, 1 part of rosemary, 0.5 part of mint, 0.5 part of stabilizer, 0.1 part of vitamin C, 4 parts of sweetener, and 300 parts of distilled water;

甜味剂为白砂糖3份、葡萄糖0.8份、甜菊糖苷0.2份。The sweeteners are 3 parts of white sugar, 0.8 parts of glucose, and 0.2 parts of steviol glycosides.

其他参数与制备方法和实施例1相同。Other parameters are the same as the preparation method and Example 1.

实施例5:Example 5:

选取诺丽果100份,椰蓉7份,不添加香草,其他参数与制备方法和实施例3相同。Select 100 parts of noni fruit, 7 parts of shredded coconut, no vanilla is added, and other parameters are the same as the preparation method and Example 3.

实施例6;Example 6;

选取诺丽果100份,不添加香草、椰蓉,其他参数与制备方法和实施例3相同。100 portions of noni fruit were selected without adding vanilla or coconut. Other parameters were the same as the preparation method and Example 3.

实施例7:Example 7:

选取诺丽果100份,香草荚10份,不添加其他罗勒、迷迭香和薄荷,其他参数与制备方法和实施例3相同。Select 100 parts of noni fruit and 10 parts of vanilla pod, without adding other basil, rosemary and mint. Other parameters are the same as the preparation method and Example 3.

实施例8:Example 8:

制备步骤省略乙醇-水溶液浸泡,其他参数与制备方法和实施例3相同。The preparation step omits soaking in ethanol-water solution, and other parameters are the same as the preparation method and Example 3.

实施例9:Example 9:

制备步骤活性炭吸附步骤,其他参数与制备方法和实施例3相同。Preparation steps: activated carbon adsorption step, other parameters are the same as the preparation method and Example 3.

实施例10:Example 10:

菌种更换为乳酸菌和嗜热链球菌,重量比为1:1,接种量为诺丽果混合物重量的0.4%,其他参数与制备方法和实施例3相同。The bacterial species were replaced with lactic acid bacteria and Streptococcus thermophilus, the weight ratio was 1:1, the inoculum amount was 0.4% of the weight of the noni fruit mixture, and other parameters were the same as the preparation method and Example 3.

对比例1:Comparative example 1:

选取诺丽果100份,稳定剂0.01份,甜味剂4份,蒸馏水100份。Select 100 parts of noni fruit, 0.01 part of stabilizer, 4 parts of sweetener, and 100 parts of distilled water.

甜味剂为葡萄糖1.95份、D-山梨糖醇2份、甜菊糖苷0.05份。The sweeteners are 1.95 parts of glucose, 2 parts of D-sorbitol, and 0.05 parts of steviol glycosides.

制备方法:Preparation:

步骤1:筛选诺丽果鲜果,选取充分成熟、汁液丰富的诺丽果,用15ppm臭氧消毒杀菌后,再用清水清洗、切片,沥干水分;Step 1: Screen the fresh noni fruits, select fully mature and juicy noni fruits, disinfect and sterilize them with 15ppm ozone, then wash them with clean water, slice them, and drain them;

步骤2:将步骤1中的诺丽果片破壁粉碎,加入蒸馏水,搅拌混合;Step 2: Crush the Noni fruit slices in Step 1, add distilled water, and stir to mix;

步骤3:在步骤2所得混合物中,加入菌种,发酵;所述菌种为保加利亚乳杆菌、鼠李糖乳杆菌和乳酸链球菌,比例为10:6:4,接种量为诺丽果混合物重量的0.4%,发酵温度为25℃,发酵时间为40D。Step 3: Add bacterial strains to the mixture obtained in step 2 and ferment; the bacterial strains are Lactobacillus bulgaricus, Lactobacillus rhamnosus and Streptococcus lactis, the ratio is 10:6:4, and the inoculum amount is the mixture of noni fruits 0.4% by weight, the fermentation temperature is 25°C, and the fermentation time is 40D.

步骤4:将步骤3所得混合物灭菌,过滤;Step 4: Sterilize the mixture obtained in step 3 and filter;

步骤5:加入添加剂,调节口味,再次灭菌灭酶,灌装、消毒,得到诺丽果酵素。Step 5: Add additives to adjust the taste, sterilize and sterilize the enzyme again, fill and sterilize, and obtain Noni enzyme.

异味感官评价:Sensory evaluation of odor:

8-10分:无异味;8-10 points: no odor;

5-7分:稍有异味,总体可以接受;5-7 points: Slightly smelly, generally acceptable;

2-4分:异味比较明显;2-4 points: The peculiar smell is relatively obvious;

0-1分:异味明显,难以接受。0-1 points: The peculiar smell is obvious and unacceptable.

结果如表1所示:The results are shown in Table 1:

表1:Table 1:

样品sample 评分score 样品sample 评分score 样品sample 评分score 实施例1Example 1 1010 实施例5Example 5 88 实施例9Example 9 55 实施例2Example 2 99 实施例6Example 6 66 实施例10Example 10 77 实施例3Example 3 1010 实施例7Example 7 77 对比例1Comparative example 1 11 实施例4Example 4 99 实施例8Example 8 44 ———— ————

测定实施例1,3中诺丽果果汁总黄酮含量,分别为35.7mg/g,37.2mg/g。The total flavonoid content of noni fruit juice in Examples 1 and 3 was measured and found to be 35.7 mg/g and 37.2 mg/g respectively.

由上表评价可知,依据本申请的方法可制得一种无异味诺丽酵素,口感更佳,适宜饮用。From the evaluation in the above table, it can be seen that a non-odorless Noni enzyme can be produced according to the method of the present application, which has a better taste and is suitable for drinking.

以上所述仅为本发明的较佳实施例而已,并不用以限制本发明,凡在本发明的精神和原则之内,所作的任何修改、等同替换、改进等,均应包含在本发明的保护范围之内。The above descriptions are only preferred embodiments of the present invention and are not intended to limit the present invention. Any modifications, equivalent substitutions, improvements, etc. made within the spirit and principles of the present invention shall be included in the present invention. within the scope of protection.

Claims (7)

1.一种无异味的诺丽果酵素,其特征在于,由以下重量份的组分组成:诺丽果100份,椰蓉5-10份,香草1-10份,添加剂2-10份和蒸馏水50-300份;所述香草为香草荚、罗勒、迷迭香和薄荷;所述一种无异味的诺丽果酵素的制备方法包括以下步骤:1. An odorless noni enzyme, characterized in that it consists of the following components by weight: 100 parts of noni, 5-10 parts of coconut, 1-10 parts of vanilla, 2-10 parts of additives and distilled water 50-300 parts; the vanilla is vanilla pod, basil, rosemary and mint; the preparation method of the noni enzyme without peculiar smell includes the following steps: 步骤1:筛选诺丽果鲜果,选取充分成熟、汁液丰富的诺丽果,用臭氧消毒杀菌后,再用清水清洗、切片,沥干水分,放入乙醇-水混合溶液,浸泡;Step 1: Screen the fresh noni fruits, select fully mature and juicy noni fruits, disinfect and sterilize them with ozone, then wash them with clean water, slice them, drain them, put them into an ethanol-water mixed solution, and soak them; 步骤2:将步骤1中的诺丽果片取出,冷冻干燥后,经破壁粉碎,加入椰蓉、香草,以及蒸馏水,搅拌混合;Step 2: Take out the noni fruit slices in step 1, freeze-dry them, crush them, add coconut mash, vanilla, and distilled water, stir and mix; 步骤3:在步骤2所得混合物中,加入菌种,发酵;所述菌种为保加利亚乳杆菌、鼠李糖乳杆菌和乳酸链球菌,接种量为诺丽果混合物重量的0.3-0.5%;Step 3: Add bacterial strains to the mixture obtained in step 2 and ferment; the bacterial strains are Lactobacillus bulgaricus, Lactobacillus rhamnosus and Streptococcus lactis, and the inoculum amount is 0.3-0.5% of the weight of the noni fruit mixture; 步骤4:将步骤3所得混合物灭菌,过滤,用含有活性炭的载体进行吸附处理;Step 4: Sterilize the mixture obtained in Step 3, filter it, and use a carrier containing activated carbon for adsorption treatment; 步骤5:加入添加剂,调节口味,再次灭菌灭酶,灌装、消毒,得到一种无异味的诺丽果酵素。Step 5: Add additives to adjust the taste, sterilize and sterilize the enzyme again, fill and sterilize, and obtain an odorless noni enzyme. 2.根据权利要求1所述无异味的诺丽果酵素,其特征在于,所述添加剂为稳定剂0.01-0.5份,维生素C 0.1-1份,余量为甜味剂。2. The odorless noni fruit enzyme according to claim 1, characterized in that the additives are 0.01-0.5 parts of stabilizer, 0.1-1 part of vitamin C, and the balance is sweetener. 3.根据权利要求2所述无异味的诺丽果酵素,其特征在于,所述甜味剂为白砂糖、葡萄糖、D-山梨糖醇、甜菊糖苷中一种或多种的混合物。3. The odorless noni fruit enzyme according to claim 2, characterized in that the sweetener is a mixture of one or more of white sugar, glucose, D-sorbitol, and steviol glycosides. 4.根据权利要求1-3任一项所述无异味的诺丽果酵素,其特征在于,由以下重量份的组分组成:诺丽果100份,椰蓉7份,香草荚4份,罗勒2份,迷迭香1份,薄荷0.5份,白砂糖2份,D-山梨糖醇1份,甜菊糖苷0.1份,稳定剂0.05份,维生素C 0.5份和蒸馏水150份。4. The odorless noni fruit enzyme according to any one of claims 1 to 3, characterized in that it is composed of the following components by weight: 100 parts of noni fruit, 7 parts of shredded coconut, 4 parts of vanilla pod, and basil. 2 parts, 1 part rosemary, 0.5 part mint, 2 parts white sugar, 1 part D-sorbitol, 0.1 part stevia glycoside, 0.05 part stabilizer, 0.5 part vitamin C and 150 parts distilled water. 5.根据权利要求1所述无异味的诺丽果酵素,其特征在于,所述步骤1中,臭氧的浓度为15-20ppm;乙醇-水混合溶液中,乙醇的浓度为60-75%wt,浸泡时间为1-2h,浸泡温度为10-20℃。5. The odorless noni fruit enzyme according to claim 1, characterized in that, in the step 1, the concentration of ozone is 15-20ppm; in the ethanol-water mixed solution, the concentration of ethanol is 60-75%wt , soaking time is 1-2h, soaking temperature is 10-20℃. 6.根据权利要求1所述无异味的诺丽果酵素,其特征在于,保加利亚乳杆菌、鼠李糖乳杆菌和乳酸链球菌的用量比为10:5-8:3-5。6. The odorless noni fruit enzyme according to claim 1, characterized in that the dosage ratio of Lactobacillus bulgaricus, Lactobacillus rhamnosus and Streptococcus lactis is 10:5-8:3-5. 7.根据权利要求1所述无异味的诺丽果酵素,其特征在于,所述步骤3中,发酵温度为15-30℃,发酵时间为25-50D。7. The odor-free noni fruit enzyme according to claim 1, characterized in that in step 3, the fermentation temperature is 15-30°C and the fermentation time is 25-50D.
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