CN113913329B - A kind of highly salt-resistant and COD-reducing bacterial strain, its acquisition method and application - Google Patents

Abstract

The present invention relates to a highly salt-resistant and COD-reducing bacterial strain. The bacterial strain is Mangrovibacter yixingensis, which is named Mangrovibacter yixingensis T3, and was preserved in the China Committee for the Preservation of Microorganisms on July 30, 2021. General Microorganism Center, the deposit number is CGMCC No.22992. The strain can survive in pharmaceutical wastewater with high salt content (≤200g/L) and high COD (10000mg/L) and exhibit good biological activity, and can consume organic waste in wastewater. The COD degradation rate of salt wastewater is over 89%, so it can be used as an active strain for biological treatment of pharmaceutical wastewater to reduce wastewater COD and improve the efficiency of activated sludge treatment of pharmaceutical wastewater. In addition, the present invention also relates to the acquisition method and application of the bacterial strain.

Description

A kind of highly salt-resistant and COD-reducing bacterial strain, its acquisition method and application

technical field

The invention relates to the technical field of applied strains, in particular to a highly salt-resistant and COD-reducing strain, its acquisition method and its application.

Background technique

The industrial output value of my country's pharmaceutical industry accounts for 2.35% of the total industrial value of the country, and the export volume of pharmaceuticals reaches more than 60%. While obtaining good economic benefits, it also produces a large amount of pharmaceutical wastewater, and its discharge accounts for 2% of the industrial wastewater discharge. , causing serious harm to the environment, and at the same time becoming one of the factors restricting the development of the industry.

Pharmaceutical wastewater mainly comes from the fermentation, extraction, filtration, ion exchange, and pharmaceutical equipment cleaning wastewater generated during the R&D and production of drugs. Due to the difference in pharmaceutical raw materials and production methods, the pollutant content in wastewater varies greatly. , but it generally has the characteristics of high organic matter content, high toxicity, high salt content and poor biodegradability. On the one hand, the existence of high salt and high organic matter will corrode the operating pipelines and equipment of the enterprise, which is not conducive to the safe production of the enterprise and the development of economic benefits, and there is a serious hidden danger of leakage. Difficult to meet emission standards.

At present, the methods for treating high-salt organic pharmaceutical wastewater mainly include Fenton catalytic oxidation method, double-membrane method, forward osmosis method, etc., but these methods still have problems such as low treatment efficiency and high operating costs; Survival in a high-salt environment, which leads to poor treatment effect of activated sludge method. By cultivating and domesticating salt-tolerant strains, improving the viability and activity in high-salt environment, the treatment effect of biological method on high-salt organic pharmaceutical wastewater can be improved.

At this stage, some scholars have screened and domesticated salt-tolerant microorganisms and applied them to degrade high-salt organic wastewater. For example, Bertrand J C et al. used salt-tolerant bacteria to treat wastewater with a salt content of 6%-20%, and the COD removal rate was stable above 70%. Jiang Haifeng and others used a salt-tolerant Bacillus cereus enhanced MBR process to treat high-salt leachate, and the COD removal rate reached 71%. However, due to the extremely complex composition of pharmaceutical wastewater, in addition to high COD and high chloride ion concentration, it also contains some or all of toxic benzene, aldehydes, esters, ethers, methanol, ethanol, formic acid, protein, phosphate and other substances, halides and All kinds of antibiotics have great inhibitory and toxic effects on microorganisms, and generally microorganisms cannot grow normally; while the high salinity of wastewater will cause excessive osmotic pressure inside and outside the cells, resulting in the death of microorganisms, or inhibiting enzyme activity and affecting growth and metabolism. Therefore, there are still some difficulties in the actual isolation and screening of bacterial strains suitable for use in high-salt pharmaceutical wastewater systems to reduce wastewater COD. In addition, as far as we know, there is no relevant report on the highly salt-tolerant Yixing Mangallobacter strain in the prior art.

Contents of the invention

(1) Technical problems to be solved

In view of the above-mentioned shortcomings and deficiencies of the prior art, the present invention provides a highly salt-resistant and COD-reducing bacterial strain, which can survive in pharmaceutical wastewater with high salt content and high COD and exhibit good biological activity, and can consume Therefore, it can be used as an active strain for biological treatment of pharmaceutical wastewater to reduce wastewater COD and improve the efficiency of activated sludge treatment of pharmaceutical wastewater. In addition, the present invention also relates to the acquisition method and application of the bacterial strain.

(2) Technical solution

In order to achieve the above object, the main technical solutions adopted in the present invention include:

In the first aspect, the present invention provides a highly salt-reducing COD strain, the strain is Mangrovibacter yixingensis, named Mangrovibacter yixingensis T3, which has been deposited in China Microbiology on July 30, 2021 General Microbiology Center of the Culture Collection Management Committee, the preservation number is CGMCCNo.22992, and the preservation address is No. 3, Yard 1, Beichen West Road, Chaoyang District, Beijing.

Said bacterial strain has been confirmed to be able to survive, grow, metabolize and reproduce normally in pharmaceutical wastewater containing high concentrations of sodium chloride, and has the ability to degrade high concentrations of COD; the COD concentration of said pharmaceutical wastewater is above 10000mg/L, preferably 11300- 15950.5mg/L, sodium chloride concentration ≤ 200g/L.

In a second aspect, the present invention provides a method for obtaining highly salt-tolerant and COD-reducing bacterial strains, comprising the steps of:

S1. Domestication of salt-tolerant flora

Activated sludge was collected from the water inlet of the secondary settling tank for sewage treatment in a pharmaceutical factory in Shijiazhuang, and the sodium chloride-resistant bacteria were directional domesticated by gradually increasing the concentration of sodium chloride;

S2, screening surviving bacteria

After acclimatization for a period of time, let the sludge stand still, take a certain amount of supernatant, and carry out gradient dilution to obtain a series of gradient dilutions, and spread the gradient dilutions on broth medium plates respectively, (28-35°C) Inverted culture at constant temperature until there are obvious colonies on the broth medium plate, and record the different colony forms;

S3, Purify a single colony

Pick single colonies with different shapes, sizes, and colors from broth medium plates with appropriate gradient concentrations, inoculate them into broth medium plates, and culture by streaking multiple times until purified single colonies are obtained;

S4, screening of salt-tolerant bacteria

Separately prepare selective media with gradient sodium chloride concentration, make a bacterial suspension from a single colony after purification, and insert (inoculate with a bacterial mass concentration of 1.8%-2.2%d, preferably at a concentration of 2.0%) to the selective media In this method, cultivate at constant temperature in a shaker for 3-4 days, take samples at predetermined intervals to measure COD degradation, and screen out high-salt-resistant and COD-reducing strains.

Spread the screened high-salt and COD-resistant strain on a broth medium plate, and culture it upside down in an incubator at 37±0.5°C for 3-7 days, observe the morphological characteristics of the colony, and the morphological characteristics and physical and chemical properties of the strain , which is different from the reported Mangrovibacteryixingensis (Gram-negative), this bacterium is Gram-positive, and may be a new strain of the genus Mangrovibacteryixingensis. The DNA of the high-salt and COD-resistant strain was further extracted for 16SrDNA sequence determination. The strain was Mangrovibacteryixingensis, which was named Mangrovibacteryixingensis T3, and was preserved in the China Committee for Microorganism Culture Collection on July 30, 2021. General Microorganism Center, the deposit number is CGMCC No.22992. The strain Mangrovibacteryixingensis T3 and several other homologous bacteria with high similarity were used to construct a phylogenetic tree with MEGA7.0 software, see Figure 3. The screened strains were stored in a -80°C refrigerator using the glycerol tube method.

According to a preferred embodiment of the present invention, in S2-S3, the composition of the broth medium is: 2.8-3.2g/L beef extract, 9.5-10.5g/L peptone, 9.5-10.5g/L Sodium chloride, 18-22g/L agar, prepared after sterilization, the pH value of the broth medium is 7.0-8.0. Preferably, the broth medium is: 3g/L beef extract, 10g/L peptone, 10g/L sodium chloride, 20g/L agar, prepared after sterilization, with a pH value of 7.0-8.0.

According to a preferred embodiment of the present invention, in S4, the selection medium components are: glucose 0.8-1.2g/L (preferably 0.9-1.0g/L), ammonium chloride 0.15-0.16g/L, diphosphate Potassium hydrogen 0.03-0.04g/L, magnesium sulfate 0.046-0.0495g/L, ferrous sulfate 0.003-0.0035g/L, sodium chloride 10-50g/L; the solvent is deionized water.

S4, the conditions for constant temperature cultivation in a shaker are: 28-35° C. (preferably 30° C.), 180-220 r/min, and shaker culture for 70-80 hours.

In a third aspect, the present invention provides an application of a high-salt-reducing COD-resistant strain, the strain is Mangrovibacteryixingensis T3 strain, which is used to inoculate into a biochemical treatment system to treat organic wastewater containing high-concentration sodium chloride.

Preferably, the method of application comprises:

Step 1: amplify the culture of the bacterial strain to obtain the bacterial liquid of the amplified culture;

Extract a single colony in the liquid basal medium, shake and culture at 28-32°C (preferably 30°C) at a speed of 110-130rpm for 20-30h (preferably 24h), centrifuge at 3500-4500rpm (preferably 4000rpm ⁾ for 10min, and purify water Make bacterium suspension after washing thalline cell, bacterium suspension is with the inoculum concentration of 4.5-5.5%, transfer in the full culture medium, at 28-32 ℃, 160-200r/min shaker culture 20-30h (preferably for 24h);

Step 2: Inject the bacterial solution into a biochemical treatment system for high-salinity wastewater with an inoculum concentration of 1.8-2.2% (preferably 2%).

The liquid basal medium contains: 2.8-3.5g/L of beef extract, 8-12g/L of peptone, 20-40g/L of sodium chloride, and pH 7.0-8.0, and is prepared after sterilization.

The full medium contains: peptone 14-16g/L (preferably 15g/L), yeast powder 6.5-8.5g/L (preferably 7.5g/L), disodium hydrogen phosphate dodecahydrate 16.5-18.5g/L L (preferably 17.9g/L), potassium dihydrogen phosphate 6-7.5g/L (preferably 6.8g/L), ammonium sulfate 3.5-4.5g/L (preferably 4.0g/L), anhydrous magnesium sulfate 0.80-0.92g/L (preferably 0.87g/L), glucose 4-6g/L (preferably 5g/L), glycerol 10-15g/L (preferably 12g/L), sodium chloride 20-40g/L L (preferably 30g/L), pH7.0-8.0, prepared after sterilization.

Preferably, the COD concentration of the high-salinity wastewater is above 10000mg/L, preferably 11300-15950.5mg/L, and the sodium chloride concentration is ≤200g/L.

It is confirmed by the experimental examples that the COD removal rate of the strain for high-salinity wastewater is up to 89.23% after 48 hours, and the COD removal rate of high-salinity wastewater for 72 hours reaches 95.56%.

In this application, if it is inoculated with bacteria, the inoculation concentration refers to the mass concentration of the bacteria in the inoculation medium; if it is inoculated with bacterial liquid or bacterial suspension, it refers to the concentration of the bacterial liquid or bacterial suspension. The volume concentration, for example, inoculation with 5% bacterial liquid means that every 5 mL of bacterial liquid corresponds to (100-5) mL of culture medium or waste water to be treated.

(3) Beneficial effects

Main technical effect of the present invention is:

The activated sludge in the secondary sedimentation tank of pharmaceutical factory wastewater treatment is domesticated with high-concentration sodium chloride, and then the sludge is left to stand to extract the supernatant, which is absorbed and cultivated on a plate to obtain different forms of colonies, and single colonies are picked Purified by plate streaking to obtain a strain of Mangrovibacter yixingensis with high salt tolerance, so it was named Mangrovibacter yixingensis T3. This strain can tolerate the concentration of sodium chloride as high as 200g/L and the concentration of COD as high as 10000mg/L The above refractory wastewater, and the removal rate of COD in the wastewater can reach more than 89%, so the strain can be inoculated into the wastewater biochemical reactor of the pharmaceutical factory to degrade the high-salt wastewater, effectively improving the traditional activated sludge method for organic matter. Removal effect, and the cost is controllable.

The invention provides the source of bacteria species for the biochemical treatment of high-salt pharmaceutical wastewater, improves the biochemical treatment effect, and effectively removes the COD value of the wastewater. The strain can still reproduce rapidly in a high-salt environment, which can effectively shorten the debugging time of the biochemical process.

It has been confirmed by experiments that the strain has a strong growth ability in the environment of high concentration of sodium chloride (the maximum concentration of sodium chloride is 200g/L), and at the same time, it shows efficient degradation of organic pollutants in wastewater (when the influent COD reaches 15950.5mg /L, the 72h degradation rate is 95.56%), with high salt tolerance and high COD degradation performance. The logarithmic growth phase and stable phase of the strain are longer (21-96h), which is conducive to the rapid consumption of various organic pollutants.

The bacterial strain Mangrovibacteryixingensis T3 screened by the present invention can survive in acidic wastewater containing methanol, acetic acid, formic acid, phosphate and other substances, and provides an efficient bacterial source for the degradation of high chloride ion and high COD wastewater in pharmaceutical factories, broadening the The functional application of Yixing Mangallobacter has strong practical value.

Description of drawings

Figure 1 is the colony morphological characteristics of strain Mangrovibacteryixingensis T3 screened in the present invention.

Fig. 2 is an electron micrograph of the bacteria strain Mangrovibacteryixingensis T3 screened by the present invention.

Fig. 3 is a phylogenetic tree of strains screened by the present invention.

Fig. 4 is the growth curve (corresponding to Example 4) of the strains screened by the present invention in liquid medium with different concentrations of sodium chloride.

Fig. 5 is the degradation curve (corresponding to implementation 5) of the bacterial strain screened by the present invention to COD pharmaceutical wastewater under higher sodium chloride concentration (200g/L).

Detailed ways

In order to better explain the present invention and facilitate understanding, the present invention will be described in detail below through specific embodiments in conjunction with the accompanying drawings.

Example 1

The present embodiment is bacterial strain domestication and screening, and its method is as follows:

Step 1: Directed domestication of salt-tolerant bacteria

Activated sludge was collected from the water inlet of the secondary sedimentation tank of a pharmaceutical factory in Shijiazhuang, and the sodium chloride-resistant bacteria were directional domesticated by gradually increasing the concentration of sodium chloride. The sewage of the pharmaceutical factory includes antibiotic industrial wastewater, synthetic drug production wastewater, Chinese patent medicine production wastewater, washing water and washing wastewater in the production process of various preparations. Its organic pollution is very serious. The COD content of the comprehensive wastewater can reach 8000-10000mg/L, containing methanol, ethanol, formic acid, protein, phosphate and other substances, and the wastewater is slightly acidic.

(1) Put the activated sludge into the shake flask, adjust the pH value to 7-8, and control the dissolved oxygen to 3-5mg/L.

(2) 4 days as a cycle, gradually increase the salt content (10g/L, 20g/L, 30g/L, 40g/L, 50g/L)

Step 2: Screen for Survival Bacteria

After acclimating for a period of time, let the sludge stand for 20 minutes, take 10 mL of the supernatant, suck 1.0 mL of the supernatant into a test tube of 9 mL of water, and perform gradient dilution (10 times, 20 times, 50 times, 100 times) to obtain a series of Gradient dilution solution, spread the gradient dilution solution on the broth medium plate respectively, mark with a marker pen, and incubate at a constant temperature of 30°C until there are obvious colonies on the broth medium plate, record the different colony shapes and Bacterial color and other characteristics.

The composition of the broth medium is: 3g/L beef extract, 10g/L peptone, 10g/L sodium chloride, 20g/L agar, prepared after sterilization, and the pH value is 7.0-8.0.

The third step, purification of single colony

Pick single colonies with different shapes, sizes, and colors from broth medium plates with appropriate gradient concentrations (plates with a thinner distribution of single colonies are easy to pick single colonies), inoculate them into broth medium plates, and streak 3 times. Line culture (see step 2 for culture conditions) until a purified single colony is obtained.

The fourth step, screening of salt-tolerant bacteria

Prepare the selective medium (selective medium of 10g/L, 20g/L, 30g/L, 40g/L, 50g/L) of gradient sodium chloride concentration respectively, single bacterium colony after purification is made bacterial suspension, with 2.0% inoculum concentration (the mass concentration of bacterial strains in the culture medium is 2%) is inserted in the selection medium (liquid medium), cultivated at a constant temperature in a shaker for 4 days, and samples are taken every predetermined time to measure the COD degradation situation, The strains resistant to high salt and COD were screened out.

Select medium components: Glucose 0.94g, NH ₄ Cl 0.153g, KH ₂ PO ₄ 0.035g, MgSO ₄ 7H ₂ O 0.1g, FeSO ₄ 7H ₂ O 0.006g, NaCl 30～150g, add distilled water to 1L , adjusted to pH 7.0-7.5.

The purified single colony was made into a bacterial suspension, which was inserted into the selective basal medium. Under the conditions of 30°C and 200rpm in a shaker, the shake flask culture was carried out for 72 hours; samples were taken every 12 hours to measure the COD value, and the strains that could tolerate high salt and degrade the COD of wastewater with the best effect were screened out.

Step 5: Identification of Salt-tolerant Bacteria

Inoculate the screened bacterial strains that can tolerate high salt and degrade COD of wastewater on broth medium plates, place them upside down in a constant temperature incubator, and culture them at 30°C for 24 hours to obtain a single colony. As shown in Figure 1, the colony is regular, moist, and milky white oval in shape.

Further, after Gram staining was positive, the morphology of the colony was observed under a microscope. As shown in Figure 2, the bacterial cell was in the shape of a white cocoon (elliptical rod), with a length of about 2307.60 μm and smoother ends. The results of physical and chemical properties test show that the methyl red test is positive, the V-P test is negative, and the facultative aerobic bacteria. The optimum growth pH is 7.0-8.0.

Genomic DNA of the screened strains was further extracted. After PCR amplification using 16S rDNA universal primers, agarose gel electrophoresis was performed to confirm the PCR amplified fragments. Samples were sent for 16S rDNA sequencing. The determined sequence has the highest homology with Mangrovibacteryixingensis, with a similarity of more than 95%. However, the morphological characteristics and physical and chemical properties of this strain are different from those of Mangrovibacteryixingensis that have been reported. This bacterium is Gram-positive. It may be a new strain of the genus Mangrovibacter yixingensis, so it was identified as a new strain of the genus Mangrovibacter yixingensis, named Mangrovibacter yixingensis T3, and it has been preserved in the General Microorganism Center (CGMCC) of the China Committee for Microbial Culture Collection on July 30, 2021 , and the deposit number is CGMCC No.22992. Mangrovibacter yixingensis T3 and several other homologous bacteria with high similarity were used to construct a phylogenetic tree with MEGA7.0 software, as shown in Figure 3. According to relevant information, there are no articles or reports about the ability of Mangallobacter Yixing to degrade high concentration COD in high concentration sodium chloride wastewater. Then the screened Mangrovibacteryixingensis T3 was made into a glycerol cryopreservation tube and stored at -80°C.

Example 2

In this example, Mangrovibacteryixingensis T3 is used to determine the degradation ability of organic matter in high-salt pharmaceutical wastewater:

The water quality of high-salt pharmaceutical wastewater from a pharmaceutical factory in Shijiazhuang: the influent COD is 11300.61mg/L, pH is 7.43, SS is 205.04mg/L, TN is 1120mg/L, and the chloride ion content is relatively high at 22649 mg/L. Poor biodegradability.

The application process is as follows:

In the first step, the Mangrovibacteryixingensis T3 strain is first amplified and cultivated to obtain the amplified cultured bacterial liquid.

Extract a single colony in the liquid basal medium, shake and culture at 30°C for 24 hours at 120rpm, centrifuge at ^4000rpm for 10min, wash the bacterial cells with pure water, and make a bacterial suspension (OD value 1.20), the bacterial suspension with The inoculum concentration was 5%, transferred to the complete medium, and cultured on a shaker at 160-200 r/min at 30° C. for 24 hours (the OD value at this time was 2.17).

The liquid basal medium contains: beef extract 3g/L, peptone 10g/L, sodium chloride 30g/L, pH 7.0, distilled water, and is sterilized.

The full medium contains: peptone 15g/L, yeast powder 7.5g/L, disodium hydrogen phosphate dodecahydrate 17.9g/L, potassium dihydrogen phosphate 6.8g/L, ammonium sulfate 4.0g/L, anhydrous sulfuric acid Magnesium 0.87g/L, glucose 5g/L, glycerin 12g/L, sodium chloride 30g/L, pH 7.0, distilled water, sterilized.

In the second step, add the high-salt organic wastewater to be treated into a 1000mL conical flask to the scale line, inoculate the bacterial solution obtained in the first step into the conical flask at a volume ratio of 2%, and control the temperature at 30 ℃, in an incubator with a rotating speed of 120rpm, and the culture was shaken, and samples were taken every 12 hours to determine its COD value.

Degradation test results: the COD value of the initial water sample was 11300.61 mg/L, and after 48 hours the COD value was 689.34 mg/L, and the COD removal rate was 93.9%.

Example 3

In this example, Mangrovibacteryixingensis T3 is used to determine the degradation ability of organic matter in high-salt pharmaceutical wastewater:

The water quality of high-salt pharmaceutical wastewater from a pharmaceutical factory in Shijiazhuang: the influent COD is 15950.50mg/L, pH is 8.31, SS is 198.69mg/L, and the chloride ion content is relatively high at 21160mg/L. The water quality is poor in biodegradability.

The process is as follows:

Add the high-salt wastewater from the pharmaceutical factory into the biochemical reactor, adjust the pH value to 7-8, turn on the aeration pump, adjust the air flow, and control the dissolved oxygen to 3-5mg/L. The bacterium solution obtained by the enlarged culture in Example 2 was inserted into a biochemical reactor with a 2% inoculation concentration (98mL waste water per 2mL) to treat high-salt waste water from a pharmaceutical factory, and the COD was measured every 24h for 4 consecutive days.

Degradation test results: the initial COD was 15950.50mg/L. At 48h, the COD removal rate was 89.23%. At 72h, the COD removal rate reached 95.56%.

Example 4

This embodiment is to test the tolerance performance and growth curve of Mangrovibacteryixingensis T3 in a liquid medium with a higher sodium chloride concentration.

Liquid medium composition: beef extract 3g/L, peptone 10g/L, sodium chloride 50-200g/L (50g/L, 150g/L, 200g/L), pH 7.0, distilled water.

As shown in Figure 4, in the liquid medium of 200/L to bacterial strain, still can propagate rapidly (lag phase is short (0-21h), logarithmic growth phase and stable phase are long (21-96h), It can effectively shorten the debugging time of the biochemical process.

Example 5

This embodiment is to test Yixing Mangelobacter Mangrovibacteryixingensis T3 under higher sodium chloride concentration to the determination of waste water organic matter degradation ability:

Wastewater quality of a pharmaceutical factory in Shijiazhuang: influent COD is 12130.60mg/L, pH is 7.43, SS is 205.04mg/L, and the salt content is 200g/L.

The process is as follows:

Add the high-salt wastewater from the pharmaceutical factory into the biochemical reactor, adjust the pH value to 7-8, turn on the aeration pump, adjust the air flow, and control the dissolved oxygen to 3-5mg/L. The bacterium solution obtained by the enlarged culture in Example 2 was inserted into a biochemical reactor at a 2% inoculation concentration (98mL waste water per 2mL) to treat high-salt waste water from a pharmaceutical factory, and the COD was measured every 24h for 7 consecutive days.

As shown in Figure 5, the degradation test results: the initial COD is 12130.60mg/L. At 3d, the COD removal rate was 47.83%. On the 7th day, the COD removal rate reached 75.49%.

Finally, it should be noted that: the above embodiments are only used to illustrate the technical solutions of the present invention, rather than limiting them; although the present invention has been described in detail with reference to the foregoing embodiments, those of ordinary skill in the art should understand that: It is still possible to modify the technical solutions described in the foregoing embodiments, or perform equivalent replacements for some or all of the technical features; and these modifications or replacements do not make the essence of the corresponding technical solutions deviate from the technical solutions of the various embodiments of the present invention. scope.

Claims (6)

1. A high-salt-tolerance COD-reducing strain is characterized in that the strain is Yixing bacillus natto (Mangreovibacter yixinensis), is named Mangrovibacter yixingensis T and is preserved in China general microbiological culture Collection center (CGMCC) with the preservation number of 22992 and the preservation address of North Chen Xiu No. 1 and No. 3 in the Yangyang area of Beijing city at the year 7 and 30 of 2021.

2. The use of the high salt tolerance COD reducing strain of claim 1, wherein the strain is Mangrovibacter yixingensis T strain for inoculation into biochemical treatment system to treat high concentration sodium chloride wastewater.

3. The use according to claim 2, wherein the high concentration sodium chloride wastewater is pharmaceutical wastewater.

4. The application according to claim 2, characterized in that the method of application comprises:

step 1: amplifying and culturing Mangrovibacter yixingensis T strain to obtain amplified and cultured bacterial liquid:

extracting Mangrovibacter yixingensis T single colony in a liquid basal medium, shake culturing at 28-32deg.C and rotation speed of 110-130rpm for 20-30h, centrifuging at 3500-4500rpm for 10min, washing thallus cells with purified water to obtain bacterial suspension, inoculating the bacterial suspension into a whole medium at inoculation concentration of 4.5-5.5%, and shake culturing at 28-32deg.C and rotation speed of 160-200r/min for 20-30h;

step 2: inoculating the bacterial liquid into a biochemical treatment system of the high-salinity wastewater at the inoculation concentration of 1.8-2.2%.

5. The use according to claim 4, wherein the liquid basal medium comprises: 2.8-3.5g/L of beef extract, 8-12g/L of peptone, 20-40g/L, pH 7.0.7.0-8.0 of sodium chloride, and sterilizing to obtain the final product;

the whole culture medium contains: 14-16g/L peptone, 6.5-8.5g/L yeast powder, 16.5-18.5g/L disodium hydrogen phosphate dodecahydrate, 6-7.5g/L potassium dihydrogen phosphate, 3.5-4.5g/L ammonium sulfate, 0.80-0.92g/L anhydrous magnesium sulfate, 4-6g/L glucose, 10-15g/L glycerol, 20-40g/L sodium chloride and pH 7.0-8.0.

6. The use according to claim 2, wherein the high concentration sodium chloride wastewater has a COD concentration of 10000mg/L or more and a sodium chloride concentration of 200g/L or less.

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