CN113854294A

CN113854294A - Disinfectant composition for musk deer breeding houses as well as preparation method and application of disinfectant composition

Info

Publication number: CN113854294A
Application number: CN202111249262.8A
Authority: CN
Inventors: 李斐然; 王艳; 杨超; 唐婕
Original assignee: SHAANXI INSTITUTE OF ZOOLOGY
Current assignee: SHAANXI INSTITUTE OF ZOOLOGY
Priority date: 2021-10-26
Filing date: 2021-10-26
Publication date: 2021-12-31

Abstract

The invention discloses a disinfectant composition for a musk deer breeding house, a preparation method and an application thereof, wherein the disinfectant composition for the musk deer breeding house comprises the following substances in percentage by mass: 0.1 to 16 percent of N, N-bis (3-aminopropyl) dodecylamine, 0.5 to 5 percent of phenoxyethanol, 0.1 to 3 percent of ethylhexyl glycerol, 3 to 10 percent of sodium pyrithione solution, 2 to 8 percent of propylene glycol, 0.1 to 2 percent of glutamic acid N, N-diacetic acid tetrasodium and the balance of deionized water. The disinfectant composition for the musk deer breeding house prepared by the invention combines the living habits of musk deer and the characteristics of breeding places, adopts the compatibility and the compounding of materials such as a quick bactericide, a high-efficiency mildew preventive, a sterilization synergist, a green chelating agent, a skin-friendly deodorant and the like, and has the advantages of simple preparation process, comprehensive protection, strong synergism, high cost performance and safe toxicology.

Description

Disinfectant composition for musk deer breeding houses as well as preparation method and application of disinfectant composition

Technical Field

The invention belongs to the field of preparation of chemical disinfectants and agricultural cultivation, and particularly relates to a disinfectant composition for a musk deer breeding house, and a preparation method and application thereof.

Background

Musk is widely concerned as an important traditional Chinese medicine, the price of musk is already over gold, and the value of one musk is over two ten thousand yuan. Although the musk deer are cultivated in Shaanxi, Sichuan, Anhui and other places in China in an initial scale, the musk deer cultivation technology needs to be deeply researched, particularly, the musk deer are seriously damaged, usually die due to unknown reasons, and the cultivation enthusiasm of the musk deer is seriously influenced. The musk deer is half-raised in a scattered way and cage-raised, and captive breeding easily causes disease transmission, so that the musk deer has high death rate. The musk deer has a relatively fixed living field and living habit, usually lives in mountainous areas with the altitude of more than 1000 meters, is covered by cloud and fog, is rainy and moist, and is easy to generate fungi (particularly mold) in a colony house except harmful bacteria. The musk deer usually forages food at night and hide in the shade of the colony house for rumination and rest in the daytime, so that the environmental sanitation requirement of the colony house is high. The smell sense of musk deer is very sensitive, and the smell sense is used for distinguishing feed, paths, excrement and even identifying male and female adults. Therefore, the sheds of the musk deer do not have external peculiar smell, so that the used disinfectant does not have special smell; in addition, the musk deer are easy to hurt the hoof due to fighting or jumping, or the hoof is softened and invaded by necrotic bacilli or other fungi to cause infection of the wound of the hoof, and if the musk deer are not treated in time, the life safety of the musk deer can be endangered.

The present species used for environmental disinfection in the musk deer farm comprise 5 to 20 percent of bleaching powder solution, 10 to 20 percent of lime milk, 5 percent of lysol and 2 to 5 percent of formalin; the soil or ground in the colony house is disinfected and sterilized by chloramine B with effective chlorine content of 4-5%, or bleaching powder, lime cream, etc., see Wujiayan, etc. and the book 'Chinese musk deer'. Among the disinfectants, the lime cream has strong alkalinity and poor effect; lysol has a pronounced phenolic taste; formalin has strong irritation and strong odor toxicity, and is not suitable for people and musk; loxamine B and bleaching powder have a pungent chlorine odor and are highly toxic. These disinfectants are completely unsuitable for disinfecting musk deer-type cultivation sites.

Other popular bactericides such as iodophors and peroxides (peroxyacetic acid, hydrogen peroxide and the like) are also high in oxidizability, can be quickly consumed when meeting organic matters such as excrement and the like to reduce the efficiency, are high in price, have strong peculiar smell and are not suitable for disinfection of captive musk deer. The other famous disinfectant chlorine dioxide is also difficult to use because of the strong smell and the need of on-site preparation. Cationic bactericides such as quaternary ammonium salts and guanidines, wherein the quaternary ammonium salts generate foams when used, are inconvenient to use and have special bitter almond flavor; guanidines such as polyhexamethylene biguanide, chlorhexidine salts and the like, although tasteless, belong to low-efficiency disinfectants, and are large in dosage and low in cost performance. Salicylic acid and its salts, benzoic acid and its salts, and sorbic acid and its salts are highly safe but effective only under acidic conditions and are extremely limited in effect. PCMX (p-chloro-m-dimethylphenol), TCS (triclosan), TCC (triclocarban), and the like have high bactericidal efficiency, but are too small in water solubility to be used with a large amount of organic solvents or surfactants. Therefore, the above disinfectant is obviously not suitable for disinfection and sterilization of musk type places.

The control of musk hoof disease usually adopts antibiotics on affected parts, but the antibiotics are expensive, and the long-term use of the antibiotics causes drug resistance of the antibiotics, and develops more aggressive harmful microorganisms. The sheep hoof disease is treated by washing the hoof with 1.10% copper sulfate solution for 1-2 minutes in a literature report, but the treatment is not applicable due to the alertness and the violent character of the musk deer, and forced treatment is not carried out on the musk deer due to the inexistence.

CN201811109623.7 discloses a botanical extract sterilization deodorant for animal husbandry sterilization, which relates to a sterilization deodorant, and comprises the following components in parts by weight: 30-50 parts of folium artemisiae argyi, 10-20 parts of cape jasmine, 5-10 parts of selfheal, 6-15 parts of mushroom essence, 3-12 parts of mint, 4-12 parts of dried orange peel, 5-20 parts of thick-stem dryopteris fragrans and 2-8 parts of saponin. For colony house disinfection, a higher sterilization effect can be achieved only after the colony house disinfection is carried out for 60 minutes, and the natural substance extract has unstable source, smell, limited effect and extremely low cost performance; CN201610763875.6 discloses a compound active iodine disinfectant for animals, which comprises the following components in percentage by mass: 0.5-3 percent of iodine, 0.1-2 percent of potassium iodate, 0.5-3 percent of potassium iodide, 20-50 percent of nonionic surfactant, 1-5 percent of organic acid, 1-5 percent of cosolvent, 5-20 percent of inorganic acid and the balance of purified water.

Most commercially available disinfectant compositions, while suitable for killing microorganisms on surfaces, or alternatively for disinfecting and disinfecting houses, are difficult to adapt to the life habits or physiological characteristics of musk deer. Also, the efficacy of many disinfectant compositions decreases rapidly after use and, because the disinfectant composition degrades rapidly, evaporates or is physically removed, it does not continue to maintain a very good germicidal effect, and if the surface is re-contaminated, the disinfectant must be reapplied to kill newly deposited microorganisms. Therefore, a disinfectant which is efficient, odorless, good in water solubility, safe in toxicology, high in cost performance, convenient to use, long in disinfection and sterilization duration, and capable of disinfecting a colony house and treating the foot injury is urgently needed, and is suitable for the special breeding environment of musk deer and the life habits of musk deer.

Disclosure of Invention

In order to overcome the defects of the prior art, the invention aims to provide a musk deer breeding house disinfectant composition, a preparation method and an application thereof, so as to overcome the defects that the existing disinfectant has short duration of disinfection and sterilization, large peculiar smell, poor disinfection efficiency, high use cost, incapability of considering both barn disinfection and hoof injury treatment, and incapability of adapting to special breeding environments of musk deer and life habits of musk deer.

In order to achieve the purpose, the invention adopts the following technical scheme to realize the purpose:

the invention discloses a disinfectant composition for a musk deer breeding house, which comprises the following components in percentage by mass: 0.1 to 16 percent of N, N-bis (3-aminopropyl) dodecylamine, 0.5 to 5 percent of phenoxyethanol, 0.1 to 3 percent of ethylhexyl glycerol, 3 to 10 percent of sodium pyrithione solution, 2 to 8 percent of propylene glycol, 0.1 to 2 percent of glutamic acid N, N-diacetic acid tetrasodium and the balance of deionized water.

Preferably, the musk deer breeding house disinfectant composition comprises the following components in percentage by mass: 8 to 12 percent of N, N-bis (3-aminopropyl) dodecylamine, 2 to 3 percent of phenoxyethanol, 0.5 to 1 percent of ethylhexyl glycerol, 5 to 10 percent of sodium pyrithione solution, 6 to 8 percent of propylene glycol, 0.1 to 0.5 percent of glutamic acid N, N-diacetic acid tetrasodium and the balance of deionized water.

Preferably, the mass fraction of the sodium pyrithione solution is 40%.

Preferably, the effective content of the N, N-bis (3-aminopropyl) dodecylamine is 90-95%.

Preferably, the pH value of the disinfectant composition for the musk breeding house is 7.5-8.

The invention also discloses a preparation method of the disinfectant composition for the musk deer breeding house, which comprises the following steps:

s1: mixing N, N-bis (3-aminopropyl) dodecylamine with deionized water, and stirring until the generated viscous colloid is completely dissolved to obtain a mixed solution;

s2: adding sodium pyrithione and tetrasodium glutamate N, N-diacetate into the mixed solution, and stirring until the mixed solution is dissolved to obtain a mixed solution A;

s3: dissolving ethylhexyl glycerol and phenoxyethanol in propylene glycol to obtain a mixed solution B;

s4: and mixing the mixed solution A and the mixed solution B under stirring, adjusting the pH value to 7.5-8 by adopting a small amount of propionic acid, adding deionized water to obtain a light yellow liquid, and filtering the light yellow liquid to obtain the musk deer breeding house disinfectant composition.

The invention also discloses application of the disinfectant composition for the musk deer breeding house, which is used as a disinfectant for disinfecting the musk deer breeding house and preventing and treating musk deer hoofs and is diluted by sterile water to the weight percentage concentration of 0.5-3.0% when in use.

Preferably, the disinfectant composition for the musk deer breeding house is sprayed to the musk deer breeding house once a week, wherein the weight percentage concentration of the disinfectant composition is 0.5% -1.0%.

Preferably, when the disinfectant composition of the musk deer breeding house is used for controlling musk deer hoofs, the weight percentage concentration is 1.0% -3.0%, and the disinfectant composition is applied to an affected part once a day by adopting a dipping or water gun spraying mode.

Compared with the prior art, the invention has the following beneficial effects:

the invention discloses a musk deer breeding house disinfectant composition, which utilizes the synergistic effect of various components to ensure that the components exert the optimal disinfection and sterilization effects, and realizes the advantage complementation according to the antibacterial spectrum of related bactericides. The N, N-bis (3-aminopropyl) dodecylamine in the disinfection and sterilization composition is scientifically compatible with the sodium pyrithione with remarkable fungicidal effect, so that a composite disinfection and sterilization agent which can realize quick disinfection and sterilization and long-term bacteriostatic effect and has better adaptability is formed; in addition, the N, N-bis (3-aminopropyl) dodecylamine in the disinfectant composition of the musk deer breeding house disclosed by the invention has high activity, and particularly has strong bactericidal capability when being used in a neutral or slightly alkaline environment, and also has strong killing effect on tiny organisms causing musk deer diseases; the phenoxyethanol in the components has strong killing effect on gram-negative bacteria, and especially has obvious effect on the pseudomonas aeruginosa causing the musk hoof disease; the ethylhexyl glycerin in the components has excellent skin compatibility, good toxicological safety and synergy with other components, simultaneously, because of the high boiling point (325 ℃), the sterilization components are locked in a certain time, the sterilization time is greatly prolonged, and because the ethylhexyl glycerin is an amphiphilic molecule (has certain surface activity, the HLB value is about 5), the ethylhexyl glycerin can be contacted with the amphiphilic molecule on the bacterial cell membrane, so as to loosen the bacterial cell membrane, lead the phenoxyethanol, the sodium pyrithione and the N, N-bis (3-aminopropyl) dodecylamine to be rapidly contacted or enter the microbial cell for sterilization, and obviously enhance the sterilization and disinfection effects; the invention discloses a disinfectant composition for a musk deer breeding house, which comprises the following components of GLDA (glutamic acid N, N-tetrasodium diacetate)) And is a green chelating agent which is more easily degraded than the conventional chelating agent. Since iron is an important element for growth and reproduction of fungi, GLDA chelates Fe³⁺The stability constant of the compound is high, so that the compound can prevent mould from obtaining iron element to breed and grow, and the good chelating capacity of the compound to iron ions can make the growth of the fungus microorganism difficult; the musk deer breeding house disinfectant composition disclosed by the invention is low in cost, small in addition amount and almost tasteless; compared with the traditional sterilizing disinfectant, the disinfectant composition for the musk deer breeding house disclosed by the invention has higher toxicological safety and is more suitable for the breeding requirements of musk deer.

Furthermore, the pH value of the disinfectant composition for the musk deer breeding house disclosed by the invention is 7.5-8, the application range is wide, the effect is best under neutral to alkalescent conditions, and the disinfectant composition is just suitable for the physiological characteristics of musk deer and most livestock.

The preparation method of the musk deer breeding house disinfectant composition disclosed by the invention is simple to operate, good in compatibility and storage performance, and easy to realize large-scale production.

The disinfectant composition for the musk deer breeding house disclosed by the invention can be used for disinfecting musk deer breeding house, can be used for preventing and controlling musk deer hoof diseases and killing harmful microorganisms (such as ticks and the like), and has an effective concentration of 0.5-3.0%. The organic combination of the fungicide, the time-delay synergist, the skin affinity agent, the deodorant, the chelating agent, the fungicide and the like is combined scientifically, the musk deer feeding place is protected from comprehensive disinfection, the harmful microorganisms are effectively prevented from generating drug resistance to the sterilizing disinfectant, and the sterilizing safety and the comprehensive guarantee are high.

Furthermore, experiments prove that the disinfectant composition for the musk deer breeding house disclosed by the invention has obvious disinfection and protection effects on a large number of microorganisms and various large-scale breeding places.

Further, the disclosed musk rearroom disinfectant compositions may be applied to the locus by any suitable method, for example, the compositions may be sprayed or otherwise applied to the surface of an object to be disinfected, and the method of application is simple.

Detailed Description

In order to make the technical solution of the present invention better understood by those skilled in the art, the technical solution will be clearly and completely described below with reference to the embodiments of the present invention. It is to be understood that the described embodiments are merely illustrative of some, and not all, embodiments of the invention. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.

It is noted that the terms first, second and the like in the description and in the claims of the present invention are used for distinguishing between similar elements and not necessarily for describing a particular sequential or chronological order. It is to be understood that the data so used is interchangeable under appropriate circumstances such that the embodiments of the invention described herein are capable of operation in sequences other than those described herein. Furthermore, the terms "comprises," "comprising," and "having," and any variations thereof, are intended to cover a non-exclusive inclusion, such that a process, method, system, article, or apparatus that comprises a list of steps or elements is not necessarily limited to those steps or elements expressly listed, but may include other steps or elements not expressly listed or inherent to such process, method, article, or apparatus.

The invention is described in further detail below:

example 1

The preparation method of the disinfectant composition for the musk deer breeding house comprises the following process steps:

mixing 10 kg of N, N-bis (3-aminopropyl) dodecylamine (Y-12) and 50 kg of deionized water, and stirring until the generated viscous colloid is completely dissolved to obtain a mixed solution;

5 kg of 40% Sodium Pyrithione (SPT) liquid and 0.2 kg of glutamic acid N, N-tetrasodium diacetate (GLDA) were added to the above mixed solution and stirred to be completely dissolved; then adding a mixed solution consisting of 0.5 kg of ethylhexyl glycerol (EHG), 1.6 kg of Phenoxyethanol (POE) and 8 kg of propylene glycol (PDO), and stirring for dissolving to obtain a light yellow transparent liquid;

adding a small amount of propionic acid into the yellowish transparent liquid to adjust the pH value to 7.9, adding the balance of water, and filtering to obtain 100 kg of the musk deer breeding house disinfectant composition.

Examples 2 to 8, comparative example 1 and comparative example 2 the manufacturing method and manufacturing steps were the same as those of example 1 except that the materials used were different in quality from those of example 1, and the specific formulations are shown in table 1.

TABLE 1 Material proportioning for examples and comparative examples

The implementation effect is as follows:

the situation of killing the mixed bacteria on the spot of the musk colony house is as follows:

the method comprises the following steps: wiping the wall surface of the musk house and the edge of a drinking water tank by using a normal saline cotton swab, and putting the musk house and the drinking water tank into a triangular flask filled with 300 g of normal saline to shake uniformly to serve as a bacteria liquid to be tested;

putting 1ml of the bacterial liquid to be tested into a plate containing a conventional culture medium (nutrient agar culture medium), and recording as a positive control;

50ml of the remaining bacterial suspension to be tested are taken into a triangular flask, and 1ml of the disinfectant composition of the musk breeding house prepared in example 1 and sterile water are added in a ratio of 1: 10, shaking up the mixed solution, then putting 1ml of the mixed solution into a plate containing a culture medium, and recording as example 1;

the same procedure as described above was used to prepare microbicidal test samples of examples 2-6, comparative example 1, comparative example 2 and commercial microbicides (10% PHMB mixed with 5% quaternary ammonium salt).

All the vessels are sterile, the culture medium is a commercial nutrient agar culture medium, the culture is carried out at 37 ℃, the viable bacteria count is carried out, and the results are shown in the following table 2:

TABLE 2 killing effect of examples and comparative examples and commercial fungicides on the field bacteria of the musk colony house (colony count cfu/g)

The killing test of the on-site mixed bacteria shows that the examples 1 to 6 show extremely excellent sterilization effect, the sterilization speed is high, and the sterilization duration is long (the sterilization effect is still kept unchanged for 3 days); comparative example 1 no additional effect of ethylhexyl glycerol and phenoxyethanol, little growth rebound of the bacteria occurs with time; in comparative example 2, the fungicide sodium pyrithione and the auxiliary fungicide tetrasodium glutamate N, N-diacetate were not present, the bactericidal effect was relatively low, and the increase of colonies was evident after 24 hours; the commercial germicide shows similar phenomenon to that of comparative example 2, and has foam when applied, which is also a common phenomenon of the conventional cationic germicide, and the germicide can sterilize rapidly but cannot last for a long time.

Preventing and treating effect on musk and hoof injury and infection

After 5 musk deer with hoof injury are washed and disinfected by 2 percent (weight percentage) of the musk deer breeding house disinfectant composition prepared by the invention in half a year, 1 percent of the musk deer breeding house disinfectant composition prepared by the invention is sprayed to an affected part by a pressure water gun every 2 days. After insisting for half a month, the forest musk deer with the hoof injury gradually recovers, and the cure rate is 100%.

Results of killing Aspergillus niger and fungi in colony house

And (5) performing a mould killing test on the musk deer culture room. The house of musk deer was sterilized with the composition of the present invention obtained in the musk deer housing according to example 1, except that the comparative 2-chamber house of musk deer was treated with the same concentration of the commercial cationic mixture as the sterilizing agent. The killing frequency is once a week, and the killing solution with the concentration of 0.8% is sprayed. After one month in summer, the colony house treated by the disinfectant composition for the musk deer breeding colony house does not generate mould, has partial mould pollution to the forest musk deer breeding houses, and is difficult to operate due to foam generation during the sun-drying process. The killing test of Aspergillus niger was performed in the laboratory on a tiger red medium with a 0.8% concentration of the disinfectant, and the results are shown in Table 3 below.

TABLE 3 results of killing Aspergillus niger by the example and comparative fungicides

The results show that the disinfectant compositions for the musk breeding houses prepared in example 1 and example 2 have excellent fungicidal effect at a concentration of 0.8%. Comparative example 2 and commercial cationic fungicides are less effective against aspergillus niger and have a significantly insufficient persistence, with the possibility of being contaminated again with moulds over time. In fact, examples 1-2 were all examples with relatively low levels of biocidal ingredient, and examples 3-6 are believed to have greater biocidal activity.

The test for killing microorganisms (mites, ticks, etc.) and the like with the disinfectant composition for a musk deer breeding house according to the present invention cannot be carried out due to the conditions. Reference is made here to the acaricidal results of N, N-bis (3-aminopropyl) dodecylamine (Lonzaba 12.100) used in the prevention and control of parasitic diseases in the center of the prevention and control of Chinese diseases, in an amount of 1mg/cm²The effects are shown in Table 4.

TABLE 4 acaricidal Effect of N, N-bis (3-aminopropyl) dodecylamine

Using the concentration ppm	The killing rate of acarid%
		500	78.03
1000	97.61
		1500	100
Control	0

One of the main components of the disinfectant composition for the musk deer breeding house is N, N-bis (3-aminopropyl) dodecylamine. According to the proportion in the example 1, when the concentration of the composition is 1%, the content of N, N-bis (3-aminopropyl) dodecylamine is over 1000ppm, and the composition has strong killing effect on tick, mite and the like which cause forest musk deer diseases.

The disinfectant composition for the musk deer breeding house can completely kill harmful microorganisms in the musk deer breeding house by using 1% of the disinfectant composition, has a good treatment effect on musk deer hoof injuries, and also has a killing effect on microorganisms causing musk deer diseases. After more than one year of practical application practice, the sterilizing agent is proved to have reliable effect, high performance-price ratio and good toxicological safety, and is very suitable for sterilizing and disinfecting musk deer breeding places.

Although the invention has been described in detail with reference to certain preferred embodiments, the examples and illustrations presented herein are for illustrative purposes only, and it is to be understood that the invention is not limited to those precise embodiments and illustrations.

The above-mentioned contents are only for illustrating the technical idea of the present invention, and the protection scope of the present invention is not limited thereby, and any modification made on the basis of the technical idea of the present invention falls within the protection scope of the claims of the present invention.

Claims

1. The musk deer breeding house disinfectant composition is characterized by comprising the following components in percentage by mass: 0.1 to 16 percent of N, N-bis (3-aminopropyl) dodecylamine, 0.5 to 5 percent of phenoxyethanol, 0.1 to 3 percent of ethylhexyl glycerol, 3 to 10 percent of sodium pyrithione solution, 2 to 8 percent of propylene glycol, 0.1 to 2 percent of glutamic acid N, N-diacetic acid tetrasodium and the balance of deionized water.

2. The disinfectant composition for musk deer breeding houses according to claim 1, wherein the disinfectant composition comprises the following components in percentage by mass: 8 to 12 percent of N, N-bis (3-aminopropyl) dodecylamine, 2 to 3 percent of phenoxyethanol, 0.5 to 1 percent of ethylhexyl glycerol, 5 to 10 percent of sodium pyrithione solution, 6 to 8 percent of propylene glycol, 0.1 to 0.5 percent of glutamic acid N, N-diacetic acid tetrasodium and the balance of deionized water.

3. A musk deer breeding house disinfectant composition according to claim 1 or 2, wherein the mass fraction of the sodium pyrithione solution is 40%.

4. A musk deer breeding house disinfectant composition according to any one of claims 1 to 3, wherein the pH of the musk deer breeding house disinfectant composition is between 7.5 and 8.

5. A process for the preparation of a musk deer colony disinfectant composition according to any one of claims 1 to 4, comprising the steps of:

s4: and mixing the mixed solution A and the mixed solution B under stirring, adjusting the pH value to 7.5-8, adding deionized water to obtain a light yellow liquid, and filtering the light yellow liquid to obtain the musk deer breeding house disinfectant composition.

6. Use of a musk deer breeding house disinfectant composition according to any one of claims 1 to 4 as a musk deer breeding house disinfectant for the disinfection of musk deer breeding houses and the control of musk deer hoofs, when in use, the composition is diluted with sterile water to a concentration of 0.5 to 3.0% by weight.

7. Use of a composition as a disinfectant for musk deer breeding houses according to claim 6, wherein the composition is sprayed once a week at a concentration of 0.5-1.0% by weight for disinfecting musk deer breeding houses.

8. The use of a composition as disinfectant for musk deer breeding houses according to claim 6, wherein the composition is applied to the affected parts once a day by dipping or spraying with a water gun, wherein the composition has a concentration of 1.0-3.0% by weight when used for controlling musk deer hooves.