[go: up one dir, main page]

CN113832063A - Preparation method and application of streptomyces coprostanus BWL-H1 microbial inoculum - Google Patents

Preparation method and application of streptomyces coprostanus BWL-H1 microbial inoculum Download PDF

Info

Publication number
CN113832063A
CN113832063A CN202111156058.1A CN202111156058A CN113832063A CN 113832063 A CN113832063 A CN 113832063A CN 202111156058 A CN202111156058 A CN 202111156058A CN 113832063 A CN113832063 A CN 113832063A
Authority
CN
China
Prior art keywords
bwl
streptomyces
microbial inoculum
preparing
preparation
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
CN202111156058.1A
Other languages
Chinese (zh)
Other versions
CN113832063B (en
Inventor
邢梦玉
刘铜
张�成
姜志超
王睿
杨艳
曹凤勤
董存柱
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Hainan University
Original Assignee
Hainan University
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Hainan University filed Critical Hainan University
Priority to CN202111156058.1A priority Critical patent/CN113832063B/en
Publication of CN113832063A publication Critical patent/CN113832063A/en
Application granted granted Critical
Publication of CN113832063B publication Critical patent/CN113832063B/en
Active legal-status Critical Current
Anticipated expiration legal-status Critical

Links

Images

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/20—Bacteria; Culture media therefor
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N63/00—Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
    • A01N63/20—Bacteria; Substances produced thereby or obtained therefrom
    • A01N63/28—Streptomyces
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N3/00—Spore forming or isolating processes

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Health & Medical Sciences (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Biotechnology (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • Virology (AREA)
  • Biomedical Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Environmental Sciences (AREA)
  • Plant Pathology (AREA)
  • Pest Control & Pesticides (AREA)
  • Dentistry (AREA)
  • Agronomy & Crop Science (AREA)
  • Medicinal Chemistry (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Agricultural Chemicals And Associated Chemicals (AREA)

Abstract

本发明提供一种粪生链霉菌BWL‑H1菌剂的制备方法和应用，粪生链霉菌BWL‑H1菌剂的制备，包括以下步骤：S1粪生链霉菌BWL‑H1固体发酵、S2制备可湿性粉剂，本发明选用麦麸和蚯蚓粪对其进行固体发酵以产生菌粉，通过生物相容性测定及国标法筛选出适合粪生链霉菌BWL‑H1的载体和助剂，其产生的孢子含量可达9.6×109CFU/g，本发明的菌剂能抑制病原微生物的生长，减少病害发生，对黄瓜枯萎病具有较高的防效效果，减少化肥农药的使用，为进一步推广应用奠定理论基础。

Figure 202111156058

The invention provides a preparation method and application of a Streptomyces faecalis BWL-H1 bacterial agent, and the preparation of the Streptomyces faecalis BWL-H1 bacterial agent comprises the following steps: S1 Streptomyces faecalis BWL-H1 solid fermentation, S2 preparation can Wet powder, the present invention selects wheat bran and vermicompost to carry out solid fermentation on it to produce bacterial powder, and screens out carriers and auxiliary agents suitable for Streptomyces faecalis BWL-H1 through biocompatibility assay and national standard method, and the spores produced by it are The content can reach 9.6×10 9 CFU/g. The inoculum of the invention can inhibit the growth of pathogenic microorganisms, reduce the occurrence of diseases, has a high control effect on cucumber fusarium wilt, reduces the use of chemical fertilizers and pesticides, and lays a foundation for further popularization and application. theoretical basis.

Figure 202111156058

Description

Preparation method and application of streptomyces coprostanus BWL-H1 microbial inoculum
Technical Field
The invention relates to the technical field of microbial fermentation, in particular to a preparation method and application of a streptomyces copromogenes BWL-H1 microbial inoculum.
Background
For a long time, pesticides and fertilizers are very important agricultural production data, and are the main ways for stabilizing and even increasing the yield of grains. The phenomenon of chemical fertilizer and pesticide abuse is ubiquitous throughout the world. Not only wastes resources, but also seriously harms the ecological environment and the health of people. The microbial agent is an important biological control technology for replacing chemical fertilizers and pesticides. Biocontrol bacteria represented by actinomycetes have been widely used for controlling plant diseases because of their excellent biocontrol effects. The research on related biocontrol bacterium preparations is carried out in China early, the biocontrol bacterium preparations are used for preventing diseases, protecting seedlings and increasing yield, and great benefits are brought to agricultural production.
Disclosure of Invention
Therefore, the invention provides a preparation method and application of a streptomyces copromogenes BWL-H1 microbial inoculum, which are used for solving the problems.
The technical scheme of the invention is realized as follows: a preparation method of a streptomyces copromogenes BWL-H1 microbial inoculum comprises the following steps:
s1, solid fermentation of streptomyces coprostanus BWL-H1: taking wormcast and wheat bran as substrates, inoculating streptomyces copromosus BWL-H1 on the substrates, fermenting for 4-10 days at 25-30 ℃, drying for 20-30H at constant temperature of 30-40 ℃, crushing and sieving with a 100-300-mesh sieve for later use to obtain spore powder;
s2, preparing wettable powder: mixing the spore powder with a wetting agent, a dispersing suspending agent and a carrier to prepare wettable powder, namely the streptomyces copromogenes BWL-H1 microbial inoculum, wherein the wettable powder comprises the following raw materials in percentage by mass: 8-12% of spore powder, 3-8% of wetting agent, 3-8% of dispersing suspending agent and the balance of carrier.
Further, the mass ratio of the wormcast to the wheat bran in the S1 is 2-5: 1.
Further, the wettable powder in the S2 comprises the following raw materials in percentage by mass: 10% of spore powder, 5% of wetting agent, 5% of dispersing and suspending agent and the balance of carrier.
Further, the wetting agent is one of sodium dodecyl benzene sulfonate, sodium dodecyl sulfate, nekal BX and AB-3, and the mass concentration is 1-5%.
Further, the dispersing suspending agent is sodium methylene dinaphthalene sulfonate (NNO) or sodium lignin sulfonate, and the mass concentration is 1-5%.
Further, the carrier is one of diatomite, kaolin and white carbon black.
Further, the prepared streptomyces copromosus BWL-H1 microbial inoculum is applied as the control effect of cucumber fusarium wilt.
Compared with the prior art, the invention has the beneficial effects that:
the streptomyces copromogenes BWL-H1 strain separated from soil at the earlier stage of the invention has the advantages of rapid growth and reproduction, rich sporulation, strong stress resistance and stable genetic character. The invention selects wheat bran and wormcast to carry out solid fermentation on the wheat bran and wormcast so as to produce bacterial powder, and the spore content can reach 9.6 multiplied by 109CFU/g, then screening out carriers and auxiliary agents suitable for the streptomyces coproagula BWL-H1 through biocompatibility determination and a national standard method, namely carrier white carbon black, wetting agent sodium dodecyl benzene sulfonate and dispersing agent NNO, preparing wettable powder according to the steps that 10% of streptomyces coproagula BWL-H1 spore powder, 5% of wetting agent sodium dodecyl benzene sulfonate, dispersing agent NNO 5% and the balance of carrier white carbon black are supplemented to 100%, and the wettable powder has a high prevention effect on cucumber fusarium wilt.
The streptomyces copromogenes BWL-H1 is prepared into the microbial inoculum, so that the growth of pathogenic microorganisms is inhibited, the occurrence of diseases is reduced, the use of chemical fertilizers and pesticides is reduced, and the theoretical basis is laid for further popularization and application.
Drawings
FIG. 1 Effect of different vectors on the growth of colonies of Streptomyces copromogenes BWL-H1;
FIG. 2 effect of different wetting agents on the growth of colonies of Streptomyces copromogenes BWL-H1;
FIG. 3 Effect of different dispersing suspending agents on the growth of colonies of Streptomyces copromogenes BWL-H1.
Detailed Description
In order to better understand the technical content of the invention, specific examples are provided below to further illustrate the invention.
The experimental methods used in the examples of the present invention are all conventional methods unless otherwise specified.
The materials, reagents and the like used in the examples of the present invention can be obtained commercially without specific description.
The streptomycete strain of the invention: streptomyces faecalis BWL-H1 is separated from soil under litchi trees in the mountain of Bawang Ling of Changjiang county, Hainan province and stored in research center of green agricultural biological preparation creation engineering in Hainan province.
The cucumber fusarium wilt of the invention is as follows: fusarium GF, isolated from diseased cucumber species of Sichuan academy of agricultural sciences, was maintained in the research center for the creation of green agricultural biologicals in Hainan province.
Example 1
A preparation method of a streptomyces copromogenes BWL-H1 microbial inoculum comprises the following steps:
s1, solid fermentation of streptomyces coprostanus BWL-H1: taking wormcast and wheat bran as a substrate, wherein the mass ratio of wormcast to wheat bran is 2:1, inoculating streptomyces copromogenes BWL-H1 on the substrate, fermenting for 4 days at 25 ℃, drying for 20 hours at constant temperature of 30 ℃, crushing and sieving with a 100-mesh sieve for later use to obtain spore powder;
s2, preparing wettable powder: 8 percent of spore powder, 3 percent of sodium dodecyl benzene sulfonate, 3 percent of methylene dinaphthalene sodium sulfonate and 86 percent of white carbon black are mixed according to the mass percentage to prepare wettable powder, namely the streptomyces coproagulans BWL-H1 microbial inoculum.
Example 2
A preparation method of a streptomyces copromogenes BWL-H1 microbial inoculum comprises the following steps:
s1, solid fermentation of streptomyces coprostanus BWL-H1: taking wormcast and wheat bran as a matrix, wherein the mass ratio of wormcast to wheat bran is 5:1, inoculating streptomyces copromogenes BWL-H1 on the matrix, fermenting at 30 ℃ for 10 days, drying at 40 ℃ for 30 hours, crushing, and sieving with a 300-mesh sieve for later use to obtain spore powder;
s2, preparing wettable powder: 12% of spore powder, 8% of sodium dodecyl benzene sulfonate, 8% of sodium methylene dinaphthalene sulfonate and 72% of white carbon black by mass percentage are mixed to prepare wettable powder, namely the streptomyces coprostanus BWL-H1 microbial inoculum.
Example 3
A preparation method of a streptomyces copromogenes BWL-H1 microbial inoculum comprises the following steps:
s1, solid fermentation of streptomyces coprostanus BWL-H1: taking wormcast and wheat bran as a matrix, wherein the mass ratio of wormcast to wheat bran is 3:1, inoculating streptomyces copromogenes BWL-H1 on the matrix, fermenting at 27 ℃ for 7 days, drying at the constant temperature of 30-40 ℃ for 24 hours, crushing, and sieving with a 200-mesh sieve for later use to obtain spore powder;
s2, preparing wettable powder: 10 percent of spore powder, 5 percent of sodium dodecyl benzene sulfonate, 5 percent of methylene dinaphthalene sodium sulfonate and 80 percent of white carbon black are mixed according to the mass percentage to prepare wettable powder, namely the streptomyces coproagulans BWL-H1 microbial inoculum.
Example 4
This example differs from the fact 3 in that the support is diatomaceous earth and the process conditions for the remaining steps are unchanged.
Example 5
This example differs from the fact 3 in that the support is kaolin and the process conditions for the remaining steps are unchanged.
Example 6
This example differs from the fact 3 in that the wetting agent is sodium lauryl sulfate and the process conditions for the remaining steps are unchanged.
Example 7
This example differs from the fact 3 in that the wetting agent is nekal BX, the process conditions for the remaining steps being unchanged.
Example 8
This example differs from the fact 3 in that the dispersing and suspending agent is sodium lignosulfonate and the process conditions for the remaining steps are unchanged.
Comparative example 1
Streptomyces faecalis BWL-H1 is prepared into Streptomyces faecalis BWL-H1 microspheres according to the Chinese plant Pathology society, research methods of research on biocontrol actinomycete spore sustained-release microspheres, Zhangwei, Fenghao et al.
First, verification experiment
(1) Spore content determination
The spore powder prepared by fermentation of examples 1-3 was measured for spore content, and the results are shown in table 1 below:
Figure BDA0003288419050000041
Figure BDA0003288419050000051
it can be seen that the spore content of example 3 can reach 9.6X 109CFU/g, indicates that fermentation at a specific temperature and time can produce a larger amount of spores.
(2) Screening of vectors
Adding the diatomite, the kaolin and the white carbon black into PDA according to the concentration of 500 mu g/ml, sterilizing at the high temperature of 121 ℃ for 20min, and then pouring the product into a flat plate for natural cooling for later use.
Culturing Streptomyces faecalis BWL-H1 for 7 days, adding 2ml of sterile water into the culture dish, slowly scraping and washing with a sterile glass slide, sucking a small amount of the sterile water on a blood counting chamber with a pipette, taking the sterile glass slide under a microscope for counting, and diluting the stock solution on a superclean bench by corresponding times to obtain the spore with the concentration of 103CFU/mL spore suspension, using a pipette gun to add 100. mu.L to PDA plate, using a sterile plate coater to spread the suspension evenly, culturing at 28 ℃ for 5 days, counting the colony number, the results are shown in Table 2 below.
TABLE 2 influence of different vectors on the colony growth of Streptomyces coproagulans BWL-H1
Figure BDA0003288419050000052
The results of the study showed no significant difference between the three vectors and ck. However, silica and diatomaceous earth showed stronger colony formation promoting effects compared to kaolin (table 2, fig. 1). In the research, the white carbon black has higher fineness and better suspension performance on the product, so in comparison of examples 3-5, the white carbon black in example 3 is more suitable to be used as a carrier of a streptomyces coprostanus BWL-H1 solid preparation.
(2) Screening of wetting Agents
Based on 2.5g, spore powder accounts for 10%, and the content of humectant is 1%, 3%, and 5%, and white carbon black is added to 100%. In a 250mL beaker, 100mL of standard hard water was added and the water bath was heated to 25 ℃. Weighing 2.5g of uniform sample, pouring all the sample into a beaker once on the premise of keeping the liquid level as stable as possible, starting timing at the same time, and recording the time for completely wetting. This was repeated three times, and the average was taken as the wetting time, and the wetting times of the three humectants at different concentrations are shown in Table 3.
TABLE 3 wetting characteristics of wetting agents and Effect on colony growth of Streptomyces coproagulans BWL-H1
Figure BDA0003288419050000061
As can be seen from the above table, SDS has better wettability, can reach 3.83 s at 5% concentration, but because it has a great influence on spore germination of actinomycetes, has poor biocompatibility with the strain, and completely inhibits spore germination at the concentration, SDBS is selected as a wetting agent, and the wetting time can be made to be less than 2min by adding the wetting agent.
(3) Screening of Dispersion suspensions
Referring to GB/T14825-2006, the suspension rates of two dispersing suspending agents, namely sodium methylenedinaphthalenesulfonate (NNO) in example 3 and sodium lignosulfonate in example 8 are compared, and when 2.5g is taken as a base number, spore powder accounts for 10%, the optimal wetting agent accounts for 5%, and when the content of the dispersing agent is respectively 1%, 3% and 5%, white carbon black is used for supplementing to 100%. Weighing a proper amount of sample, taking a 250mL beaker, adding 50mL of standard hard water, heating in a constant-temperature water bath to 30 +/-1 ℃, and adding the sample. Shaking the beaker by hand, continuing for 2min at a relatively gentle shaking rate, standing for 4min, pouring all the liquid into a 250mL measuring cylinder, and washing for multiple times until the scale is full. And (3) completely blocking the opening of the measuring cylinder by using a cover, shaking up and down for a period of time, and then continuously standing in the water bath kettle, wherein the spore concentration is measured to be C1. After standing for a sufficient period of time, the supernatant was aspirated without aspiration of the pellet, at which time the spore concentration in both barrels was measured to be C2. The suspension ratio is calculated as follows:
Figure BDA0003288419050000062
the suspension characteristics of the two dispersion suspending agents are shown in table 4,
TABLE 4 suspension characteristics of the Dispersion suspending agent and Effect on the growth of colonies of Streptomyces coproagulans BWL-H1
Figure BDA0003288419050000071
As can be seen from the above table, when no dispersant is added and only the spore powder is mixed into the silica carrier in an amount of 10%, the suspension rate of the preparation is only 42.25%. The suspension characteristic of the dispersant NNO is superior to that of sodium lignosulphonate, and the suspension rate of the preparation can reach 70.97%. Compared with the biocompatibility of the streptomyces copromogenes BWL-H1, NNO has no inhibition phenomenon on the spore germination of actinomycetes, so the NNO is selected as a dispersant to be added into the wettable powder.
Second, experiment of potted plant for preventing and treating cucumber fusarium wilt
Inoculating fusarium GF into a PDB flat plate, carrying out shake culture in a constant-temperature incubator at 28 ℃ for 7d, filtering hyphae through 2 layers of coarse gauze, and preparing into bacterial suspension with the spore concentration of 1 × 106CFU/mL for later use.
After accelerating germination, the cucumber seeds are sowed in a seedling tray, and are transplanted into a pot with the caliber of 10cm and the height of 9.5 after two weeks. The experiment was set up with 10 treatments: t1, clear water control; t2: diluting the streptomyces copromogenes BWL-H1 wettable powder of embodiment 3 by 100 times, and irrigating roots of cucumber seedlings by 100mL of spore suspension of fusarium GF after one week; t3, mixing the streptomyces copromogenes BWL-H1 microspheres and nutrient soil in a mass ratio of 1: 100, and repeating for 3 times each treatment, wherein 10 seedlings are repeated each time. After 10 days, the disease condition of the plants is investigated, and the prevention and treatment effect and the disease index standard are shown in Table 5.
TABLE 5 grading Standard and formula for diseases of different grades
Figure BDA0003288419050000072
Figure BDA0003288419050000081
The mean and standard deviation of the samples were calculated using AVERAGE and STDEVP functions in Excel2007, and multiple comparisons (P <0.05) were performed using waller-duncan in SPSS17.0 software as shown in table 6 below.
TABLE 6 preventive effect of two preparations of Streptomyces BWL-H1 on cucumber fusarium wilt
Figure BDA0003288419050000082
Note: t1, T2 and T3 represent control, wettable powder treatment and microsphere treatment respectively.
As can be seen from the above table, the wettable powder treatment, the microsphere treatment and the control have significant differences (P <0.05), and the control effect of the streptomyces coproanus BWL-H1 wettable powder on cucumber fusarium wilt is most obvious, can reach 48.65 percent and is significantly higher than the control effect of the streptomyces coproanus BWL-H1 microspheres on fusarium wilt.
The above description is only for the purpose of illustrating the preferred embodiments of the present invention and is not to be construed as limiting the invention, and any modifications, equivalents, improvements and the like that fall within the spirit and principle of the present invention are intended to be included therein.

Claims (7)

1. A preparation method of a streptomyces copromogenes BWL-H1 microbial inoculum is characterized by comprising the following steps: the method comprises the following steps:
s1, solid fermentation of streptomyces coprostanus BWL-H1: taking wormcast and wheat bran as substrates, inoculating streptomyces copromosus BWL-H1 on the substrates, fermenting for 4-10 days at 25-30 ℃, drying for 20-30H at constant temperature of 30-40 ℃, crushing and sieving with a 100-300-mesh sieve for later use to obtain spore powder;
s2, preparing wettable powder: mixing the spore powder with a wetting agent, a dispersing suspending agent and a carrier to prepare wettable powder, namely the streptomyces copromogenes BWL-H1 microbial inoculum, wherein the wettable powder comprises the following raw materials in percentage by mass: 8-12% of spore powder, 3-8% of wetting agent, 3-8% of dispersing suspending agent and the balance of carrier.
2. The method for preparing the Streptomyces coproagulans BWL-H1 microbial inoculum according to claim 1, which is characterized in that: and the mass ratio of the wormcast to the wheat bran in the S1 is 2-5: 1.
3. The method for preparing the Streptomyces coproagulans BWL-H1 microbial inoculum according to claim 1, which is characterized in that:
the wettable powder in the S2 comprises the following raw materials in percentage by mass: 10% of spore powder, 5% of wetting agent, 5% of dispersing and suspending agent and the balance of carrier.
4. The method for preparing the Streptomyces coproagulans BWL-H1 microbial inoculum according to claim 1 or 3, which is characterized in that: the wetting agent is one of sodium dodecyl benzene sulfonate, sodium dodecyl sulfate, nekal BX and AB-3, and the mass concentration is 1-5%.
5. The method for preparing the Streptomyces coproagulans BWL-H1 microbial inoculum according to claim 1 or 3, which is characterized in that: the dispersing suspending agent is sodium methylene dinaphthalene sulfonate or sodium lignin sulfonate, and the mass concentration is 1-5%.
6. The method for preparing the Streptomyces coproagulans BWL-H1 microbial inoculum according to claim 1 or 3, which is characterized in that: the carrier is one of diatomite, kaolin and white carbon black.
7. The Streptomyces faecium BWL-H1 microbial inoculum prepared by any one of claims 1 to 6 is applied to the control effect of cucumber fusarium wilt.
CN202111156058.1A 2021-09-29 2021-09-29 Preparation method and application of Streptomyces faecalis BWL-H1 microbial inoculum Active CN113832063B (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CN202111156058.1A CN113832063B (en) 2021-09-29 2021-09-29 Preparation method and application of Streptomyces faecalis BWL-H1 microbial inoculum

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
CN202111156058.1A CN113832063B (en) 2021-09-29 2021-09-29 Preparation method and application of Streptomyces faecalis BWL-H1 microbial inoculum

Publications (2)

Publication Number Publication Date
CN113832063A true CN113832063A (en) 2021-12-24
CN113832063B CN113832063B (en) 2023-11-24

Family

ID=78967751

Family Applications (1)

Application Number Title Priority Date Filing Date
CN202111156058.1A Active CN113832063B (en) 2021-09-29 2021-09-29 Preparation method and application of Streptomyces faecalis BWL-H1 microbial inoculum

Country Status (1)

Country Link
CN (1) CN113832063B (en)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN117264851A (en) * 2023-11-10 2023-12-22 中国热带农业科学院三亚研究院 Streptomyces strain and application thereof

Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN103087946A (en) * 2012-12-28 2013-05-08 云南大学 Microbial strain and application thereof
CN105394085A (en) * 2015-10-29 2016-03-16 海南大学 A kind of wettable powder of Streptomyces aureus verticalus HN6 and preparation method thereof
CN108208016A (en) * 2017-12-31 2018-06-29 海南大学 Application of the volatile materials that streptomyces fimicarius generates in control of plant disease
CN111518707A (en) * 2020-05-09 2020-08-11 山东碧蓝生物科技有限公司 Compound bacterium powder, compound microbial agent thereof, and preparation method and application thereof
CN114304190A (en) * 2021-09-29 2022-04-12 海南大学 A kind of preparation method of Trichoderma wettable powder

Patent Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN103087946A (en) * 2012-12-28 2013-05-08 云南大学 Microbial strain and application thereof
CN105394085A (en) * 2015-10-29 2016-03-16 海南大学 A kind of wettable powder of Streptomyces aureus verticalus HN6 and preparation method thereof
CN108208016A (en) * 2017-12-31 2018-06-29 海南大学 Application of the volatile materials that streptomyces fimicarius generates in control of plant disease
CN111518707A (en) * 2020-05-09 2020-08-11 山东碧蓝生物科技有限公司 Compound bacterium powder, compound microbial agent thereof, and preparation method and application thereof
CN114304190A (en) * 2021-09-29 2022-04-12 海南大学 A kind of preparation method of Trichoderma wettable powder

Non-Patent Citations (4)

* Cited by examiner, † Cited by third party
Title
MENGYU XING等: "Antifungal Activity of Natural Volatile Organic Compounds against Litchi Downy Blight Pathogen Peronophythora litchii" *
VALERIAVENTORINO等: "Lignocellulose-Adapted Endo-Cellulase Producing Streptomyces Strains for Bioconversion of Cellulose-Based Materials" *
邢梦玉: "两株链霉菌对荔枝霜疫病的防病潜力和防病机理研究" *
郭纹余等: "链霉菌挥发性化合物提高植物抗病能力的研究进展" *

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN117264851A (en) * 2023-11-10 2023-12-22 中国热带农业科学院三亚研究院 Streptomyces strain and application thereof
CN117264851B (en) * 2023-11-10 2024-02-09 中国热带农业科学院三亚研究院 Streptomyces strain and application thereof

Also Published As

Publication number Publication date
CN113832063B (en) 2023-11-24

Similar Documents

Publication Publication Date Title
CN113789274B (en) Grape rhizosphere antagonistic growth-promoting streptomyces F2 and application thereof
CN113201474B (en) Bacillus subtilis TBWR1, its application and the obtained control agent
CN110468083B (en) Separated streptomycete DL70 and biocontrol growth promotion application thereof
CN113480383A (en) Microbial fertilizer suitable for saline-alkali soil and preparation method thereof
CN112920956B (en) Beauveria bassiana BD01 strain and fermentation method and application thereof
CN109097303A (en) Paenibacillus polymyxa and its spore suspending liquid, microorganism seedling medium and preparation method thereof
CN115029280A (en) Burkholderia gladioli for antagonizing cucumber fusarium wilt and application thereof
CN112592856B (en) Tobacco brown spot antagonistic actinomycete strain and application thereof
CN113832063A (en) Preparation method and application of streptomyces coprostanus BWL-H1 microbial inoculum
CN111073827A (en) A kind of Bacillus velesi JTB8-2 and its application in the biological control of Gualedang
CN118985635A (en) Salt-tolerant biocontrol bacterial agent and preparation method and application thereof
CN111378595B (en) A Burke&#39;s agricultural biocontrol strain Ba1 and use thereof
CN110218657B (en) Trichoderma longibrachiatum MD30 and biological organic fertilizer developed by same
CN108998395B (en) A kind of Bacillus amyloliquefaciens and its application
CN118028141A (en) Bacillus bailii SF4 and application thereof
CN115305221B (en) Composite microbial agent, preparation method thereof and application thereof in preventing and treating empty heart disease of strawberries
CN113621540B (en) Bacillus clausii strain and its screening method and application
CN119162061B (en) A strain of Bacillus subtilis NM62 and its application in growth promotion and disease resistance
CN115948274B (en) A nitroreducing Pseudomonas and its application in rice drought resistance and growth promotion
CN119040221B (en) A strain of Priesterol NM17 and its application in controlling tobacco black shank disease
CN113832074B (en) Actinomycetes, and biocontrol microbial inoculum and application thereof
CN119899780B (en) A synthetic bacterial community for breaking alfalfa seed dormancy and promoting seedling growth and its application
LU502648B1 (en) Trichoderma-based seed coating agent, preparation method and application thereof
CN119899776B (en) Disease-resistant growth-promoting yield-increasing strain, composite microbial agent and application thereof
CN118813499B (en) Bacillus paralicheniformis and its application in preventing and controlling ginseng rust

Legal Events

Date Code Title Description
PB01 Publication
PB01 Publication
SE01 Entry into force of request for substantive examination
SE01 Entry into force of request for substantive examination
GR01 Patent grant
GR01 Patent grant