CN113827632B - A kind of soybean extract and preparation method and application thereof - Google Patents

Abstract

The present application relates to the field, in particular, to a soybean extract and its preparation method and application. The preparation method of soybean extract includes: breaking the soybean wall, keeping the temperature at 55-65° C. for 0.5-3 hours; then keeping the temperature at 1-4° C. for 6-15 hours, filtering and taking the filtrate; adding C1-C4 to the filtrate Alcohol and additives, adjust the electrical conductivity to 40000-80000μs/cm, then let the system stand for stratification, and take the upper layer liquid; material purification is realized during the extraction and preparation process, and protein and lecithin are efficiently removed. The electrostatic effect, hydrophobic effect and bioaffinity effect of different substances are used to form a two-phase aqueous phase, thereby affecting the solubility of different components and effectively improving the extraction rate and purity of soybean isoflavones.

Description

A kind of soybean extract and preparation method and application thereof

technical field

The present application relates to the field, in particular, to a soybean extract and its preparation method and application.

Background technique

Soy isoflavones have health-care effects of lowering cholesterol, reducing cardiovascular disease, preventing osteoporosis and relieving menopausal syndrome; soybean is the most important dietary source of soy isoflavones; but in the prior art methods for extracting isoflavones from soybeans The extraction rate needs to be improved.

SUMMARY OF THE INVENTION

The purpose of the embodiments of the present application is to provide a soybean extract and its preparation method and application, which are intended to improve the extraction rate of isoflavones extracted from soybeans.

The application provides a preparation method of soybean extract, comprising:

Break the soybean wall and keep it at 55～65℃ for 0.5～3h; then keep at 1～4℃ for 6～15h, filter and take the filtrate;

Add C1～C4 alcohol and additives to the filtrate, adjust the electrical conductivity to 40000～80000μs/cm, let it stand until the system is layered, and take the upper layer liquid;

Wherein, the additive comprises cetyl PEG/PPG-10/1 polydimethylsiloxane and/or PEG-30 dipolyhydroxystearate; the weight ratio of the additive to the soybean is 10～ 20%.

The soybean wall is broken, then kept at high temperature and then kept at low temperature to destroy the internal tissue of soybean and separate soybean isoflavones from soybean tissue; adjust the filtrate by C1-C4 alcohol and additives, so that soybean protein and lecithin and additives enter the same layer , C1-C4 alcohol and soybean isoflavones enter the same layer; material purification is realized during the extraction and preparation process, and protein and lecithin are efficiently removed. The electrostatic effect, hydrophobic effect and bioaffinity effect of different substances are used to form a two-phase aqueous phase, thereby affecting the solubility of different components and effectively improving the extraction rate and purity of soybean isoflavones.

In some embodiments of the present application, the step of breaking the soybean wall includes: grinding soybean, grinding aid and water together; the grinding aid includes at least one of calcium sulfate, calcium carbonate and barium sulfate.

In some embodiments of the present application, the soybean is soybean germ.

In some embodiments of the present application, a salt insoluble in C1-C4 alcohol is used to adjust the conductivity;

Optionally, the conductivity is adjusted with potassium chloride and/or sodium chloride.

In some embodiments of the present application, the C1-C4 alcohol is ethanol;

The volume ratio of C1-C4 alcohol to the filtrate is 1-2.

In some embodiments of the present application, in the step of taking the filtrate after filtration, a centrifugal method is used to filter and then take the filtrate.

In some embodiments of the present application, the step of maintaining the temperature at 55-65° C. for 0.5-3 h is: stirring at 55-65° C. and 500-800 rpm for 0.5-3 h.

In some embodiments of the present application, C1-C4 alcohol is added to the filtrate, and the additive step is carried out under the condition of 2000-5000 rpm.

The present application provides a soybean extract, which is prepared by the above-mentioned preparation method of a soybean extract.

The application also provides an application, the application of the above soybean extract in the preparation of anti-allergic drugs or anti-inflammatory drugs.

Description of drawings

In order to illustrate the technical solutions of the embodiments of the present application more clearly, the following briefly introduces the accompanying drawings that need to be used in the embodiments. It should be understood that the following drawings only show some embodiments of the present application, and therefore do not It should be regarded as a limitation of the scope, and for those of ordinary skill in the art, other related drawings can also be obtained according to these drawings without any creative effort.

Fig. 1 shows the physical image of the system of Example 1 after standing for 1.0h.

FIG. 2 shows the physical image of the system of Example 2 after standing for 1.0 h.

FIG. 3 shows the physical image of the system of Example 3 after standing for 1.0 h.

FIG. 4 shows the physical image of the system of Comparative Example 1 after standing for 1.0 h.

FIG. 5 shows the physical image of the system of Comparative Example 2 after standing for 1.0 h.

FIG. 6 shows the physical image of the system of Comparative Example 3 after standing for 1.0 h.

FIG. 7 shows the physical image of the system of Comparative Example 4 after standing for 1.0 h.

FIG. 8 shows the physical image of the system of Comparative Example 5 after standing for 1.0 h.

FIG. 9 shows the results of the inhibition rate of hyaluronidase by the soybean extracts of Examples 1-2 and Comparative Examples 1-5.

FIG. 10 shows the results of the inhibition rate of lipoxygenase by the soybean extracts of Examples 1-2 and Comparative Examples 1-5.

Detailed ways

To make the purposes, technical solutions and advantages of the embodiments of the present application more clear, the technical solutions in the embodiments of the present application will be described clearly and completely below. If the specific conditions are not indicated in the examples, it is carried out according to the conventional conditions or the conditions suggested by the manufacturer. The reagents or instruments used without the manufacturer's indication are conventional products that can be purchased from the market.

The soybean extracts of the embodiments of the present application and their preparation methods and applications will be specifically described below.

A preparation method of soybean extract, comprising:

Break the soybean wall and keep it at 55～65℃ for 0.5～3h; then keep at 1～4℃ for 6～15h, filter and take the filtrate;

Add C1～C4 alcohol and additives to the filtrate, adjust the conductivity to 40000～80000μs/cm, let it stand until the system is layered, and take the upper layer liquid;

Wherein, the additive is cetyl PEG/PPG-10/1 polydimethylsiloxane and/or PEG-30 dipolyhydroxystearate; the weight ratio of the additive to the soybean is 10～ 20%.

As an example, the wall is broken first, then the high-temperature heat preservation is performed, and then the low-temperature heat preservation is performed to destroy the internal tissue of the soybean so that the soybean isoflavones enter the filtrate.

In some embodiments of the present application, the step of breaking the soybean wall comprises: grinding soybean, grinding aid and water together.

For example, the grinding aid includes at least one of calcium sulfate, calcium carbonate, and barium sulfate. It can be understood that, in some other embodiments of the present application, the grinding aid can be selected from other substances that do not chemically react with soybeans, do not undergo physical adsorption and are insoluble in water.

It can be understood that, in other embodiments of the present application, other methods can be used to destroy the cell walls of soybeans, and the method is not limited to grinding.

In some embodiments of the present application, soybean is soybean germ, and the isoflavone content of soybean germ is higher, so that more soybean isoflavones can be obtained; it can be understood that in other embodiments of the present application, soybean can be directly selected.

As an example, the mass ratio of grinding aid to soybean germ is 1-4%; for example, it can be 1%, 2%, 3%, 4%.

The mass ratio of water to soybean can be (0.8-2):1, for example, it can be 0.8:1, 0.9:1, 1:1, 1.2:1, 1.5:1, 2:1 and so on.

Soybean, grinding aid and water are ground together, and the grinding aid can fully separate soybean isoflavones from other soybean tissues; after grinding, keep at 55-65℃ for 0.5-3h; then keep at 2-4℃ for 6-15h , and take the filtrate after filtration.

Incubating at 55-65°C can destroy the protein, and then denaturing the soybean protein at 1-4°C.

For example, the temperature at high temperature can be 55°C, 56°C, 57°C, 58°C, 59°C, 60°C, 63°C, 65°C, etc., and the high temperature holding time can be 0.5h, 0.8h, 1h, 1.5h , 2h, 2.5h, 3h, etc.

Illustratively, the incubation at 55-65°C may be stirring at this temperature, for example, stirring at 500-800 rpm (eg, may be 500 rpm, 600 rpm, 700 rpm, 800 rpm) at 55-65° C. for 0.5-3 h. Alternatively, the ground system is directly kept at 55-65° C. for 0.5 to 3 hours, and operations such as stirring may not be performed.

After high temperature heat preservation, low temperature heat preservation is carried out. The temperature of low temperature heat preservation is 1 to 4°C, for example, it can be 1°C, 2°C, 3°C, 4°C, etc., and the time of low temperature heat preservation is 6 to 15h, for example, it can be 6h, 7h, 8h, 9h, 10h, 11h, 12h, 15h, etc. In the process of low temperature heat preservation, operations such as stirring may not be performed.

Filtration was performed after the low temperature incubation was completed.

For example, the solid-liquid is separated by means of centrifugation, for example, centrifugation with a centrifugal force of 10,000 g.

To the filtrate were added C1-C4 alcohol, additives and mixed.

Additives include cetyl PEG/PPG-10/1 dimethicone and/or PEG-30 dipolyhydroxystearate. For example, the additive includes cetyl PEG/PPG-10/1 dimethicone; alternatively, the additive includes PEG-30 dipolyhydroxystearate; alternatively, the additive includes cetyl PEG/PPG-10/ 1 Dimethicone and /PEG-30 Dipolyhydroxystearate.

The weight ratio of additive to soybean is 10-20%; for example, it can be 10%, 11%, 12%, 13%, 15%, 16%, 18%, 19%, 20% and so on.

For example, the C1-C4 alcohol may include at least one of methanol, ethanol, propanol, isopropanol, butanol, and isobutanol. In some embodiments of the present application, the C1-C4 alcohol is ethanol.

As an example, the volume of ethanol is 1-2 times the volume of the filtrate; for example, it may be 1, 1.2, 1.5, 1.8, or 2 times, and so on.

It can be understood that, without considering factors such as the utilization rate of ethanol, the amount of ethanol can also be much more than the filtrate.

As an example, in order to fully mix the filtrate, C1-C4 alcohol, and additives, the system may be stirred at 2000-5000 rpm for 2-10 min to make the system evenly mixed.

After adjusting the conductivity to 40000～80000μs/cm, let it stand until the system is layered, and take the upper layer liquid;

Illustratively, the conductivity is adjusted using salts insoluble in C1-C4 alcohols, such as potassium chloride, sodium chloride, calcium chloride, and the like.

Adjust the conductivity to 40000 μs/cm, 50000 μs/cm, 60000 μs/cm, 70000 μs/cm, 80000 μs/cm.

For example, the amount of the salt insoluble in C1-C4 alcohol can be 20-32% by weight of the filtrate; it is understood that, in other embodiments of the present application, the amount of the salt can be selected as an appropriate amount guided by the conductivity.

After adjusting the conductivity, let it stand until the system is separated, and take the upper layer liquid.

Soy isoflavones mainly exist in the upper liquid (alcohol layer). Protein and lecithin in soybean are dissolved in the lower layer; additives can promote the dissolution of protein and lecithin in the lower layer.

In some embodiments, drying the supernatant to remove the alcohol is also included.

The preparation method of the soybean extract provided by the present application has at least the following advantages:

The method has the advantages of low cost, continuous operation and the like, and the reaction conditions are mild, and the principle of similar compatibility is used to realize substance purification during the extraction and preparation process, and efficiently remove protein and lecithin. The protein, oil and lecithin rich in soybean are solubilized by additives, and the electrostatic, hydrophobic and biological affinity of different substances are used to form a two-phase water phase, thereby affecting the solubility of different components and effectively improving soybean isoflavones extraction yield and purity.

The present application also provides a soybean extract, which is prepared by the above-mentioned preparation method of a soybean extract.

The soybean extract prepared by the aforementioned method has a higher content of soybean isoflavones and lower contents of protein and lecithin.

The application also provides an application, the application of the above soybean extract in the preparation of anti-allergic drugs or anti-inflammatory drugs.

The above soybean extract has better anti-allergic and anti-inflammatory effects, and can be used in the preparation of anti-allergic or anti-inflammatory drugs.

The features and properties of the present application will be further described in detail below with reference to the embodiments.

Example 1

The present embodiment provides a soybean extract, mainly obtained by the following steps:

(1) Take 1kg of soybean germ, add 0.02kg of calcium sulfate, put it into a three-roller, add 1.02kg of water, pulverize, and repeat three times to form a homogenate.

(2) Take the homogenate obtained in step (1), add 20 times of water, heat up to 60°C, stir at 500 rpm for 0.5h, cool down to 4°C for 10h, centrifuge at 10,000g, and take the supernatant.

(3) Add anhydrous ethanol that is 1 times the weight of the supernatant, add cetyl PEG/PPG-10/1 polydimethylsiloxane of 15% of the weight of soybean germ, stir at 4000 rpm for 5 minutes, add the weight of soybean germ 4.5 times of potassium chloride, stir until completely dissolved, and then let stand for 1.0h. Figure 1 shows the physical image after standing for 1.0h, and obvious delamination can be seen.

(4) Take the upper layer liquid (this is the ethanol layer), carry out vacuum drying, and obtain soybean extract.

Example 2

The present embodiment provides a soybean extract, mainly obtained by the following steps:

(1) Take 1kg of soybean germ, add 0.02kg of calcium sulfate, put it into a three-roller, add 1.02kg of water, pulverize, and repeat three times to form a homogenate.

(2) Take the homogenate obtained in step (1), add 10 times water, heat up to 60°C, stir at 500 rpm for 0.5h, cool down to 4°C for 10h, centrifuge at 10,000g, and take the supernatant.

(3) add the absolute ethanol of 2.0 times the weight of the supernatant, add the 15% PEG-30 dimerized hydroxystearate of the soybean germ weight, stir 5min at 5000 rpm, add 5 times the potassium chloride of the soybean germ weight, stir It can be completely dissolved, and then let stand for 1.0h. Figure 2 shows the physical picture after standing for 1.0h, and obvious delamination can be seen.

(4) Take the upper layer liquid (this is the ethanol layer), carry out vacuum drying, and obtain soybean extract.

Example 3

The present embodiment provides a soybean extract, mainly obtained by the following steps:

(1) Take 1kg of soybean germ, add 0.02kg of calcium sulfate, put it into a three-roller, add 1.02kg of water, pulverize, and repeat three times to form a homogenate.

(2) Take the homogenate obtained in step (1), add 20 times of water, heat up to 60°C, stir at 500 rpm for 0.5h, cool down to 4°C for 10h, centrifuge at 10,000g, and take the supernatant.

(3) Add methanol of 1 times the weight of the supernatant, add 15% cetyl PEG/PPG-10/1 polydimethylsiloxane of the soybean germ weight, stir at 4000 rpm for 5 min, add 4.5% of the soybean germ weight times of potassium chloride, stir until completely dissolved, and then let stand for 1.0h. Figure 3 shows the physical image after standing for 1.0h, and obvious delamination can be seen.

(4) Take the upper layer liquid (this is the methanol layer) and vacuum dry to obtain soybean extract.

Comparative Example 1

This comparative example provides a soybean extract, which is mainly prepared through the following steps:

(1) Take 1kg of soybean germ, put it into a three-roller, add 1kg of water, and pulverize, repeat three times to form a homogenate.

(2) Take the homogenate obtained in step (1), add 15 times of water, heat up to 60°C, stir at 500 rpm for 0.5h, cool down to 4°C for 10h, centrifuge at 10,000g, and remove the supernatant.

(3) Add anhydrous ethanol 2.0 times the weight of the supernatant, add 15% cetyl PEG/PPG-10/1 polydimethylsiloxane or PEG-30 dimer hydroxystearate by weight of soybean germ , Stir at 2000-5000rpm for 5min, add potassium chloride 4 times the weight of soybean germ, stir until completely dissolved, and then let stand for 1.0h. Figure 4 shows the physical image after standing for 1.0h, and obvious delamination can be seen.

(4) Take the upper layer liquid (this is the ethanol layer), carry out vacuum drying, and obtain soybean extract.

Comparative Example 2

This comparative example provides a soybean extract, which is mainly prepared through the following steps:

(1) Take 1kg of soybean germ, add 0.02kg of calcium sulfate, put it into a three-roller, add 1.02kg of water, pulverize, and repeat three times to form a homogenate.

(2) Take the homogenate obtained in step (1), add 25 times of water, heat up to 60°C, stir at 500 rpm for 0.5h, cool down to 3°C for 10h, centrifuge at 10,000g, and take the supernatant.

(3) Add anhydrous ethanol twice the weight of the supernatant, stir at 2000-5000 rpm for 5 min, and then let stand for 1.0 h. Figure 5 shows the physical picture after standing for 1.0h, and it can be seen that there is no delamination.

(4) after filtration, take the filtrate and vacuum-dry to obtain soybean extract.

Comparative Example 3

This comparative example provides a soybean extract, which is mainly prepared through the following steps:

(1) Take 1 kg of soybean germ, add 0.02 calcium sulfate, put it into a three-roller, add 1.02 kg of water, and pulverize, repeat three times to form a homogenate.

(2) Take the homogenate obtained in step (1), add 15 times of water, heat up to 60° C., stir at 500 rpm for 0.5 h, centrifuge at 10,000 g, and take the supernatant.

(3) add absolute ethanol twice the weight of the supernatant, add 20% cetyl PEG/PPG-10/1 polydimethylsiloxane by weight of soybean germ, stir at 5000 rpm for 5min, add 4% by weight of soybean germ times of potassium chloride, stir until completely dissolved, and then let stand for 1.0h. Figure 6 shows the physical image after standing for 1.0h, and obvious delamination can be seen.

(4) Take the upper layer liquid (this is the ethanol layer), carry out vacuum drying, and obtain soybean extract.

Comparative Example 4

This comparative example provides a soybean extract, which is mainly prepared through the following steps:

(1) Take soybean germ, add 0.02kg of calcium carbonate, put it into a three-roller, add 1:1 water, pulverize, repeat three times to form a homogenate.

(2) Take the homogenate obtained in step (1), add 25 times of water, heat up to 60°C, stir at 500 rpm for 0.5h, cool down to 3°C for 10h, centrifuge at 10,000g, and take the supernatant.

(3) Add absolute ethanol 1.0 times the weight of the supernatant, add 35% cetyl PEG/PPG-10/1 polydimethylsiloxane by weight of soybean germ, stir at 5000 rpm for 5 minutes, add 2.5% by weight of soybean germ times of potassium chloride, and then let stand for 1.0h. Figure 7 shows the physical image after standing for 1.0h, and it can be seen that there is no delamination.

(4) After filtration, the filtrate is taken and vacuum-dried to obtain soybean extract.

Comparative Example 5

This comparative example provides a soybean extract, which is mainly prepared through the following steps:

(1) Take soybean germ, add 0.02kg of calcium carbonate, put it into a three-roller, add 1:1 water, pulverize, repeat three times to form a homogenate.

(2) Take the homogenate obtained in step (1), add 20 times of water, heat up to 60°C, stir at 500 rpm for 0.5h, cool down to 3°C for 10h, centrifuge at 10,000g, and take the supernatant.

(3) Add 20% cetyl PEG/PPG-10/1 polydimethylsiloxane by weight of soybean germ, stir at 5000 rpm for 5 minutes, add potassium chloride 4.5 times the weight of soybean germ, and then let stand for 1.0 h. Figure 8 shows the physical image after standing for 1.0h, and it can be seen that there is no delamination.

(4) After filtration, the filtrate is taken and vacuum-dried to obtain soybean extract.

Experimental Example 1

The protein content of the soybean extracts of Examples 1-3 and Comparative Examples 1-5 was tested by the BCA protein concentration determination method. The principle of the test method is: under alkaline conditions, the protein reduces the divalent copper ion to monovalent copper ion, and then reacts with the BCA reagent to generate a purple compound, which is detected at 562 nm. The test results are shown in Table 1.

Protein content (%)=determined sample protein content (g)/soybean extract freeze-dried powder mass (g)

Table 1

group Protein content (%) Example 1 0.50 Example 2 0.49 Example 3 0.42 Comparative Example 1 1.25 Comparative Example 2 15.02 Comparative Example 3 8.09 Comparative Example 4 3.50 Comparative Example 5 12.0

Experimental example 2

The content of soybean isoflavones in the soybean extracts of test examples 1-3 and comparative examples 1-5, the test principle is as follows:

The structure of each component of soybean isoflavone contains hydroxyl and aromatic ring functional groups, forming a conjugated system with strong ultraviolet absorption characteristics. The maximum absorption wavelength difference of each component of soybean isoflavone is very small, and the maximum absorption wavelength of genistein in the ultraviolet region is 260nm. , The maximum absorption wavelength of daidzein is 256nm, and the content of soybean isoflavones can be detected by ultraviolet spectrophotometry. Taking genistein as the standard substance, the absorbance value was measured at 260 nm, and the standard curve was y=107.79x-0.0014, R ² =0.9999. The test results are shown in Table 2.

Table 2

It can be seen from Experimental Example 1, Experimental Example 2 and Example 3:

Compared with Example 1, no grinding aid was added in Comparative Example 1, resulting in low extraction rate and low isoflavone content in soybean extract.

In Comparative Example 2, no additives and salt were added, and the layers were not separated after standing, resulting in poor separation of protein and soybean isoflavones. Therefore, the proportion of soybean isoflavones in the soybean extract of Comparative Example 2 was small and the purity was low. , with a large proportion of protein.

In Comparative Example 3, after stirring at 60° C., but not incubating at low temperature (1-4° C.), the proportion of isoflavones and protein in the soybean extract of Comparative Example 3 were large, and the two were not separated.

Additives were added in Comparative Example 4. The amount of additives was 35% of the weight of soybean germ. The amount of additives was too large, resulting in poor separation of protein and soybean isoflavones. The proportion of flavonoids in soybean extract was small, and the proportion of protein was large. .

In Comparative Example 5, ethanol was not added, resulting in failure to form two phases, resulting in low flavonoid purity. The proportion of flavonoids in soybean extract is relatively small, and the proportion of protein is relatively large.

Experimental example 3

The antiallergic efficacy of the soybean extracts of Examples 1-3 and Comparative Examples 1-5 was tested by hyaluronidase in vitro inhibition method.

The test method includes: taking the soybean extracts of Examples 1-3 and Comparative Examples 1-5 and dissolving them in water to a concentration of 5 mg/mL;

Take 0.5mL of sample, 0.5mL of 1200U/mL hyaluronidase and 0.1mL of CaCl ₂ solution, mix, incubate for 20min, add 0.5mL of sodium hyaluronate, incubate at 37°C for 40min; add 0.1mL of sodium hydroxide and 0.5mL of acetylacetone, boil in water bath 15min, ice bath for 5min, placed at room temperature for 10min; 1.0mL of LP-DAB developed color, 3mL of absolute ethanol was added, and the absorbance was measured at 530nm after mixing.

The results of the inhibition rate of hyaluronidase by the soybean extracts of Examples 1-3 and Comparative Examples 1-5 are shown in FIG. 8 .

Hyaluronidase is a participant in allergic reactions, breaking down hyaluronic acid (HA) in the body, turning it into a low-molecular-weight acidic stimulus, causing the release of histamine and inducing allergic symptoms in the body. It can be seen from FIG. 9 that the soybean extracts provided in Examples 1-3 have excellent hyaluronidase inhibitory effect.

Experimental example 4

Lipoxygenase Inhibition Experiment The anti-inflammatory effects of the soybean extracts of Examples 1-3 and Comparative Examples 1-5 were tested.

Lipoxygenase (LOX for short) is an oxidoreductase containing non-heme iron, which specifically catalyzes polyunsaturated fatty acids with cis and cis-1,4-pentadiene structures such as arachidonic acid and linoleic acid. The dioxygenation reaction of leukotrienes (LTs) and hydroxytetracostetraenoic acid (X-HETE) with conjugated unsaturated fatty acids is generated. Therefore, the anti-inflammatory activity can be reflected to a certain extent by detecting the inhibition rate of a certain substance on lipoxygenase.

The test method includes: taking the soybean extracts of Examples 1-3 and Comparative Examples 1-5 and dissolving them in water to a concentration of 5 mg/mL. Take 0.1mL of the sample extract and 0.4mL of lipoxygenase 260u/mL, mix them in a 10mL test tube, take a 30°C water bath for 3 minutes, add 0.54mL/L linoleic acid 1.6mL, mix in a 30°C water bath for 3 minutes, and then add 4mL Absolute ethanol, lipoxygenase inhibition rate was detected at 234 nm.

The results of the inhibition rate of lipoxygenase by the soybean extracts of Examples 1-3 and Comparative Examples 1-5 are shown in FIG. 10 .

It can be seen from Fig. 10 that the soybean extracts provided in Example 1, Example 2 and Example 3 have a 95.2% inhibitory effect on lipoxygenase, which is much better than the soybean extracts of Comparative Examples 1-5.

The above descriptions are only preferred embodiments of the present application, and are not intended to limit the present application. For those skilled in the art, the present application may have various modifications and changes. Any modification, equivalent replacement, improvement, etc. made within the spirit and principle of this application shall be included within the protection scope of this application.

Claims (8)

1. a preparation method of soybean extract, is characterized in that, comprises: Grinding soybean germs, grinding aids and water together, keeping the temperature at 55-65°C for 0.5-3h; then keeping at 1-4°C for 6-15h, filtering and collecting the filtrate; wherein, the grinding aids include calcium sulfate , at least one of calcium carbonate and barium sulfate; Add C1～C4 alcohol and additives to the filtrate, adjust the electrical conductivity to 40000～80000μs/cm, let it stand until the system is layered, and take the upper layer liquid; wherein, potassium chloride and/or sodium chloride are used to adjust the electrical conductivity; Wherein, the additive comprises cetyl PEG/PPG-10/1 polydimethylsiloxane and/or PEG-30 dipolyhydroxystearate; the weight ratio of the additive to the soybean is 10～ 20%. 2. the preparation method of soybean extract according to claim 1, is characterized in that, The C1-C4 alcohol is ethanol. 3. The preparation method of soybean extract according to claim 2, wherein the volume ratio of the C1-C4 alcohol to the filtrate is 1-2. 4. The preparation method of soybean extract according to any one of claims 1-3, characterized in that, in the step of taking the filtrate after the filtration, a centrifugal method is used to filter and then take the filtrate. 5. The preparation method of soybean extract according to any one of claims 1-3, wherein the step of keeping the temperature at 55-65°C for 0.5-3h is: at 55-65°C, 500-800rpm under stirring for 0.5 ~ 3h. 6. The preparation method of soybean extract according to any one of claims 1-3, wherein the step of adding C1-C4 alcohol and additives to the filtrate is carried out under the condition of 2000-5000 rpm. 7. A soybean extract, characterized in that, the soybean extract is prepared by the method for preparing a soybean extract according to any one of claims 1-6. 8. The application of the soybean extract of claim 7 in the preparation of anti-allergic drugs or anti-inflammatory drugs.

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