CN113817634B - Bacillus amyloliquefaciens Bam-6 and preparation and application thereof - Google Patents
Bacillus amyloliquefaciens Bam-6 and preparation and application thereof Download PDFInfo
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N25/00—Biocides, pest repellants or attractants, or plant growth regulators, characterised by their forms, or by their non-active ingredients or by their methods of application, e.g. seed treatment or sequential application; Substances for reducing the noxious effect of the active ingredients to organisms other than pests
- A01N25/02—Biocides, pest repellants or attractants, or plant growth regulators, characterised by their forms, or by their non-active ingredients or by their methods of application, e.g. seed treatment or sequential application; Substances for reducing the noxious effect of the active ingredients to organisms other than pests containing liquids as carriers, diluents or solvents
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
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- A01N37/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids
- A01N37/44—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids containing at least one carboxylic group or a thio analogue, or a derivative thereof, and a nitrogen atom attached to the same carbon skeleton by a single or double bond, this nitrogen atom not being a member of a derivative or of a thio analogue of a carboxylic group, e.g. amino-carboxylic acids
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- A01N63/00—Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
- A01N63/20—Bacteria; Substances produced thereby or obtained therefrom
- A01N63/22—Bacillus
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- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
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Abstract
The invention discloses bacillus amyloliquefaciens Bam-6 and a preparation and application thereof, wherein the bacillus amyloliquefaciens Bam-6 is named asBacillus amyloliquefaciens Bam‑6And has been preserved in China center for type culture Collection at 8 months and 16 days in 2021, with the preservation number being CCTCCNO: m20211040. The bacillus amyloliquefaciens Bam-6 provided by the invention has a good killing effect on peach aphids, has a strong inhibiting effect on xanthomonas carpi, rice blast pathogenic bacteria, phomopsis longipes, fusarium oxysporum and root rot pathogenic bacteria, can be directly applied by using a fermentation liquid thereof, and greatly reduces the cost.
Description
Technical Field
The invention belongs to the field of microorganisms, and particularly relates to bacillus amyloliquefaciens Bam-6 and a preparation and application thereof.
Background
The green peach aphids, also called peach red aphids, tobacco aphids, vegetable aphids, etc., belong to the family of semipterodontobius. The green peach aphids are widely distributed, are important economic pests in a plurality of temperate regions in the world, and are one of the cabbage aphids which are the most serious in China; myzus persicae feeding impurity, host plant including more than 50 families including agricultural crops (potato, beet and tobacco etc.), horticultural crops (Cruciferae, solanaceae and Cucurbitaceae etc.) and stone fruit (peach, apricot, cherry etc.); the green peach aphids are fast in propagation and parthenogenesis, and the generation period of the green peach aphids is short; the green peach aphids are seriously harmful to young tissues, flowers and fruits of plants, so that leaves are curled and wilted to influence growth and development; the green peach aphids have strong migratory flight capability, can transfer harm among various plants and spread various plant viruses; meanwhile, the green peach aphids can secrete honeydew, so that the surfaces of plant leaves are bright and luminous, plant photosynthesis is influenced, and even sooty mould parasitism can be caused; the efficient prevention and treatment of the green peach aphids are always a very headache for farmers and are also a research hotspot for scholars at home and abroad.
At the present stage, the prevention and control of the peach aphids in the agriculture and forestry production mainly depend on chemical pesticides, so that the human health and the safety of the aquaculture industry are not only directly influenced, but also the ecological balance is damaged due to the fact that the peach aphids generate drug resistance and a large amount of chemical pesticides kill natural enemies of the peach aphids, the natural control capability in the field is seriously weakened, and the peach aphids are rampant and difficult to control easily. Along with the improvement of health consciousness of people, the defects of chemical pesticides are increasingly exposed, biogenic pesticides are gradually paid attention from all circles, and the adoption of safe microbial preparations to replace chemical pesticides becomes an important development direction for aphid control. The microbial pesticide directly utilizes thalli as a using entity, has a plurality of sources of strains and low production cost, has the characteristics of environmental protection, no public nuisance, strong specificity and pathogenicity and no drug resistance compared with a chemical pesticide, and is used for preventing and controlling various agricultural and forestry pests. Therefore, the development and utilization of the novel microbial aphicide are necessary and significant.
The citrus canker is a worldwide disease which is classified as a quarantine object by more than 30 countries and regions, is a bacterial infectious disease caused by a citrus pathovar carpet xanthomonas, and has the advantages of wide transmission path, rapid infection, serious harm and stubborn and difficult prevention. The citrus canker damages citrus leaves, branches and tips and fruits, causes fallen leaves, withered branches, weak tree vigor and reduced fruit value, and seriously affects yield and quality. The research of China on citrus canker begins in the 50 th 20 th century, but at present, the prevention and treatment of citrus canker is mainly carried out by a chemical method, the varieties of medicaments are few, and the chemical pesticides such as agricultural streptomycin and copper preparations are mainly used singly and in large quantities for a long time, so that the generation of drug resistance of germs and the pollution to the environment are easily caused, great adverse effects are caused on the sustainable development of Jiangxi citrus, particularly navel orange industry, and the prevention and treatment of the citrus canker face severe examination. Therefore, the finding of the microbial bactericide with good control effect on citrus canker is an urgent research subject.
Phomopsis (Phomopsis) is a large genus of fungi of Deuteromycota, class Cycospora, order Sporochaetales, family Sporochaetaceae, containing over 100 different species, and can parasitize over 70 different families of plants. The pathogenic bacteria of the genus have wide geographical distribution, cause severe diseases of plants such as leaf withering, branch withering, rotten stems, ulcers, fruit rot and the like, and cause great economic loss. If the bacterial strain is the pathogenic strain of soft rot of picked kiwi fruit, pathogenic bacteria of kiwi fruit branch blight, stiff peach bud and plantago spike blight.
The rice blast caused by the infection of the rice blast (Magnaporthe oryzae) is listed as the first three diseases of rice, thereby seriously threatening the global food safety, causing the yield loss to be about 10 to 30 percent each year and reaching 50 percent during the disease outbreak period.
Fusarium oxysporum (Fusarium oxysporum) is a serious-harm soil-borne pathogenic fungus and is listed as one of ten world plant pathogenic fungi. The strain can infect more than 100 crops with important economic value, such as cotton (Gossypium hirsutum), soybean (Glycine max), watermelon (Citrullus lanatus), banana (Musa nana), tomato (Lycopersicon esculentum) and alfalfa (Medicago sativa), and can cause blight, root rot and the like. After the fusarium oxysporum is infected, the yield of crops can be reduced in a large area, for example, the yield of watermelons can be reduced by 20% -30%, the yield of serious fields can reach 50% -60%, and even the crops are out of production; the disease incidence rate of the banana garden is 10-40%, and the disease severity can reach more than 90% so that the banana garden is abandoned; the yield of the infected alfalfa is reduced by 20-40%, and the yield of the infected alfalfa is reduced by more than 60% in serious plots.
Disclosure of Invention
The invention obtains the multifunctional Bacillus amyloliquefaciens Bam-6 by screening, wherein the name of the Bacillus amyloliquefaciens Bam-6 is Bacillus amyloliquefaciens Bam-6, the Bacillus amyloliquefaciens Bam-6 is preserved in China center for type culture collection (CCTCC NO) at 8 months and 16 days in 2021, the preservation address is Wuhan, and the preservation number is CCTCC NO: m20211040. The gene sequence is shown as SEQ ID No:1 is shown. Experiments prove that the insecticidal composition has a good killing effect on peach aphids, and also has a strong inhibiting effect on citrus canker pathogenic bacteria xanthomonas carpi. In addition, the compound also has strong inhibiting effect on rice blast disease source bacteria, phomopsis, fusarium oxysporum and root rot disease source bacteria.
The invention also aims to provide a green peach aphid killing preparation as a microbial pesticide, which comprises the fermentation liquor of the bacillus amyloliquefaciens Bam-6. Microbial pesticide and chemical pesticide, has multiple formulations, if can directly utilize the fermentation liquor to carry out biological control, can reduce production cost and shorten production time, and then make the product more easily make people accept in the price, bring convenience for the popularization and sale of the product.
The preparation can be water agent, and the components can also comprise bacteriostatic agent; the bacteriostatic agent may be NaNO 2 、NaNO 3 、 NaN 3 And EDTA.
The invention has the beneficial effects that: the multifunctional bacillus amyloliquefaciens Bam-6 provided by the invention has a good killing effect on peach aphids, has a strong inhibiting effect on xanthomonas carpi, rice blast pathogenic bacteria, phomopsis, fusarium oxysporum and root rot pathogenic bacteria, can be directly applied by using a fermentation liquid thereof, and greatly reduces the cost.
Drawings
FIG. 1 shows a colony morphology of Bam-6 hydrolyzed starch and gelatin;
FIG. 2 shows a hydrolysis loop of Bam-6 on a cellulose plate;
FIG. 3 is a diagram showing the result of a bioassay experiment for killing Myzus persicae by Bam-6; wherein, A is a picture before the experiment group is sprayed with Bam-6 fermentation liquor, B is a picture after the experiment group is sprayed with Bam-6 fermentation liquor for 1d, C is a picture after the experiment group is sprayed with Bam-6 fermentation liquor for 1 week, E is a picture before the comparison group is sprayed with sterile water, F is a picture after the comparison group is sprayed with sterile water for 1d, and G is a picture after the comparison group is sprayed with sterile water for 1 week;
FIG. 4 is a diagram showing the result of the Bam-6 bacteriostatic test; wherein A is an inhibition graph of Bam-6 fermentation liquor on rice blast pathogenic bacteria, B is an inhibition graph of Bam-6 fermentation liquor on phomopsis, C is an inhibition graph of Bam-6 fermentation liquor on fusarium oxysporum, D is an inhibition graph of B am-6 fermentation liquor on root rot pathogenic bacteria, and E is an inhibition graph of Bam-6 fermentation liquor on Xanthomonas carpi;
FIG. 5 is a diagram showing the results of Bam-6 confrontation experiments; wherein, the left side of A is a colony diagram of rice blast disease source bacteria, the right side is a confronting diagram of Bam-6 fermentation liquor to the rice blast disease source bacteria, the left side of B is a phomopsis colony diagram, the right side is a confronting diagram of Bam-6 fermentation liquor to the phomopsis, the left side of C is a colony diagram of fusarium oxysporum, the right side is a confronting diagram of Bam-6 fermentation liquor to the fusarium oxysporum, the left side of D is a colony diagram of root rot disease source bacteria, and the right side is a confronting diagram of Bam-6 fermentation liquor to the root rot disease source bacteria.
Detailed Description
The concept and technical effects of the present invention will be clearly and completely described below in conjunction with the embodiments and the accompanying drawings to fully understand the objects, aspects and effects of the present invention.
Example 1:
the specific screening process of the multifunctional bacillus amyloliquefaciens Bam-6 comprises the following steps:
s1, primary screening: adding 10mL seawater into a triangular flask containing 90mL sterile water, placing in a shaking table at 150rpm, oscillating for 30min, taking out, and gradually diluting to 10 -1 ，10 -2 ，10 -3 ，10 -4 ，10 -5 ，10 -6 Respectively coating the 6 diluted solutions with the 6 concentrations on a sodium carboxymethylcellulose (CMC-Na) plate, culturing at 25 ℃ for 3d, inoculating on a new sodium carboxymethylcellulose culture medium after bacteria grow out, and purifying until pure strains are obtained;
s2, re-screening: the single strains separated in the preliminary screening were respectively spotted on a screening medium (CMC-Na 10g, (NH) 4 ) 2 SO 4 1.4g、MgSO 4 0.3g、KH 2 PO 4 2g、MnSO 4 1.6mg、FeSO 4 5mg、ZnSO 4 2.5mg、CoCl 2 2.0mg, agar 20g, PH7.0, constant volume of 1L), culturing at 25 ℃ for 3D, staining with 1.0g/L congo red for 3 min, pouring off the staining solution, decolorizing with 1mol/L NaCl aqueous solution for 1h, measuring colony diameter (D, cm) and hydrolysis ring diameter (D, cm), and expressing the hydrolysis capacity by Dp: dp = (D/D) 2 ；
S3, determining the activity of the cellulose degrading enzyme of the strain: inoculating the strains obtained by re-screening in fermentation enzyme production culture medium (CMC-Na 10g, (NH) with an inoculum size of 5% 4 ) 2 SO 4 4.0g、MgSO 4 ·7H 2 O 0.5g、K 2 HPO 4 2g of beef extract, 5g of beef extract and 10g of peptone, wherein the pH is natural and the constant volume is 1L), culturing for 3 days on a shaking table (25 ℃,150 rpm), centrifuging for 10min at 6000 rpm, and taking supernatant fluid, namely the prepared crude enzyme solution; the activity of the filter paper enzyme, the activity of endo-glucosidase, the activity of exonuclease and the activity of beta-glucosidase are measured by adopting 3,5-dinitrosalicylic acid colorimetric method (DNS).
The bacterium Bam-6 with better cellulose degradation energy is obtained through the screening process, the bacterial colony is faint yellow and opaque on an LB culture medium, the surface is rough, the bacterial colony has a bump and an irregular edge, a mycoderm is formed when liquid culture is static, gram staining is positive, a rod shape can form endogenetic spores, the bacterial colony is oval, two ends of the bacterial colony are blunt, the sporocyst does not expand, and the bacterium from the mesogenesis to the secondary end is provided with motility. The bacterial colony morphology of the hydrolyzed starch and gelatin is shown in FIG. 1. The Bam-6 genome was extracted and amplified using bacterial universal primer 27F: agagtttggattcctggctcag, 1492R: TACGGCTACCTTGTTACG ACTT was PCR amplified and the BLAST results on NCBI after sequencing was Bacillus amyloliquefaciens and was named: bacillus amyloliquefaciens Bam-6.
The activity of the cellulose-degrading enzyme of Bam-6 was measured, and the hydrolysis loop thereof is shown in FIG. 2, and Dp =10, indicating that it has a better cellulose-degrading ability. The enzyme activity of the cellulose endonuclease is measured to be 38u/mL by a DNS method, the enzyme activity of the filter paper enzyme is measured to be 30u/mL, the enzyme activity of the exonuclease is measured to be 32u/mL, and the enzyme activity of the beta-Gase is measured to be 28u/mL.
Example 2:
inoculating the bacillus amyloliquefaciens Bam-6 bacterial liquid obtained by screening in the example 1 into a test tube filled with 5mL of fermentation enzyme production culture medium according to the inoculation amount of 1%, culturing at 30 ℃ and 200rpm for 1d, taking 1.0mL of bacterial suspension into a 1.5mL centrifuge tube, centrifuging at 8000rpm for 5min, and taking the supernatant for later use, namely the bacillus amyloliquefaciens Bam-6 fermentation liquid.
Example 3:
the Bam-6 fermentation liquor prepared in the example 2 is used for a bioassay experiment for killing green peach aphids, and specifically comprises the following steps:
setting an experimental group and a control group, and respectively culturing the green peach aphids by using the broad bean seedlings, wherein the experimental group sprays Bam-6 fermentation liquor onto the green peach aphids by using a spray can, and the control group sprays sterile water.
After 1d, the death rate of the myzus persicae in the experimental group reaches more than 85%, the control group is unchanged, after 1 week, the death rate of the myzus persicae in the experimental group reaches more than 95%, but the myzus persicae in the control group is changed more (due to fast aphid propagation), and the results are shown in fig. 3. As can be seen from FIG. 3, the Bacillus amyloliquefaciens Bam-6 obtained by screening has good effect of killing the myzus persicae.
Example 4:
(1) The Bam-6 fermentation liquor prepared in the example 2 is used for carrying out bacteriostasis experiments, and specifically comprises the following steps:
respectively inoculating rice blast pathogenic bacteria, phomopsis longipes, fusarium oxysporum and root rot pathogenic bacteria into a test tube filled with 5mL of fungus culture liquid medium, and culturing at 30 ℃ and 200rpm for 48h to obtain respective bacterial suspensions. The citrus canker pathogenic bacteria xanthomonas carpocasiae is inoculated into a test tube filled with 5mL of NA culture liquid medium, and the bacterial suspension of the xanthomonas carpocasiae is obtained after culturing for 48 hours at the temperature of 28 ℃ and the rpm of 200.
The method comprises the following steps: respectively coating respective bacterial suspensions of 40 mu L of rice blast pathogenic bacteria, phomopsis longissima, fusarium oxysporum, root rot pathogenic bacteria and citrus canker pathogenic bacteria xanthomonas carpii on PDA and NA culture medium flat plates, sticking sterile circular Xinhua filter paper on a flat dish after 2h, respectively dripping 10 mu L of Bam-6 fermentation liquid on the filter paper, culturing at 30 ℃ for 48h, measuring the diameter of an inhibition zone, dividing the diameter of the inhibition zone by the diameter of a bacterial colony, and calculating the diameter ratio of the inhibition zone.
The results of the bacteriostatic experiments on the Bam-6 fermentation broth are shown in Table 1 and FIG. 4.
TABLE 1
As can be seen from Table 1 and FIG. 4, the Bacillus amyloliquefaciens Bam-6 obtained by screening has strong inhibiting effect on rice blast disease-causing bacteria, phomopsis, fusarium oxysporum, root rot disease-causing bacteria and citrus canker disease-causing bacteria, xanthomonas carpi.
(2) The confrontation experiment is carried out by adopting the Bam-6 fermentation liquor prepared in the example 2, and the confrontation experiment specifically comprises the following steps:
taking 4 PDA plates, drilling small holes in the central positions of the PDA plates, respectively dripping 10 mu L of bacterial suspensions of rice blast disease source bacteria, phomopsis longipes, fusarium oxysporum and root rot disease source bacteria, drilling small holes in positions 2cm away from the center, inoculating 10 mu L of Bam-6 fermentation liquor, and culturing for 48h at 30 ℃ by taking the plate which is not inoculated with the Bam-6 fermentation liquor as a reference. And when the diameter of the colony of the control group is 2/3 of that of the whole culture dish, measuring the diameter of the colony of the control group and the diameter of the colony inoculated with the Bam-6 fermentation liquor, and calculating the bacteriostasis rate of the Ba m-6 fermentation liquor to the bacteria. Bacteriostatic rate = (control colony diameter-bacteriostatic colony diameter)/control colony diameter.
The results of the bacteriostatic rates of the Bam-6 fermentation broth on rice blast pathogens, phomopsis, fusarium oxysporum and root rot pathogens are shown in Table 2 and FIG. 5.
TABLE 2
Example 5:
an antibacterial aphicide comprises 100mL of Bam-6 fermentation liquor prepared by the method of example 2 and Na NO 2 (amount of use)0.05 g/kg). The preparation process comprises the following steps: heat treating Bam-6 fermenting liquid at 100 deg.c for 30min and mixing with N aNO 2 Mixing, and storing at room temperature in dark place.
Example 6:
an antibacterial aphicide solution, comprising 100mL of Bam-6 fermentation broth prepared by the method of the embodiment 2 and Na NO 3 (the dosage is 0.5 g/kg), and the preparation process comprises the following steps: heat treating Bam-6 fermenting liquid at 121 deg.c for 10min and mixing with N aNO 3 Mixing, and storing at room temperature in dark place.
Example 7:
an antibacterial aphicide solution, comprising 100mL of Bam-6 fermentation broth prepared by the method of the embodiment 2 and Na N 3 (the dosage is 1 g/kg), and the preparation process comprises the following steps: heat treating Bam-6 fermenting liquid at 80 deg.c for 1 hr and mixing with NaN 3 Mixing, and storing at room temperature in dark place.
Example 8:
an antibacterial aphicide comprises 100mL of Bam-6 fermentation liquor prepared by the method of example 2 and 3g/kg of E DTA, and the preparation process comprises the following steps: the Bam-6 fermentation liquor is obtained by mixing with EDTA after heat treatment for 15h at 100 ℃, and then is stored at room temperature in a dark place.
The above description is only a preferred embodiment of the present invention, and the present invention is not limited to the above embodiment, and the present invention shall fall within the protection scope of the present invention as long as the technical effects of the present invention are achieved by the same means. The invention is capable of other modifications and variations in its technical solution and/or its implementation, within the scope of protection of the invention.
SEQUENCE LISTING
<110> institute of microbiology of academy of sciences of Jiangxi province
<120> Bacillus amyloliquefaciens Bam-6 and preparation and application thereof
<130> 2021
<160> 1
<170> PatentIn version 3.5
<210> 1
<211> 1463
<212> DNA
<213> Bacillus amyloliquefaciens Bam-6
<400> 1
aaatctgtca cttcggcggc tggctccata aaggttacct caccgacttc gggtgttaca 60
aactctcgtg gtgtgacggg cggtgtgtac aaggcccggg aacgtattca ccgcggcatg 120
ctgatccgcg attactagcg attccagctt cacgcagtcg agttgcagac tgcgatccga 180
actgagaaca gatttgtggg attggcttaa cctcgcggtt tcgctgccct ttgttcttgt 240
ccattgtagc acgtggtgta gcccaggtca taaggggcat gatgatttga cgtcatcccc 300
accttcctcc ggtttgtcac cggcagtcac cttagagtgc ccaactgaat gctggcaact 360
aagatcaagg gttgcgctcg ttgcgggact taacccaaca tctcacgaca cgagctgacg 420
acaaccatgc accacctgtc actctgcccc cgaaggggac gtcctatctc taggattgtc 480
agaggatgtc aagacctggt aaggttcttc gcgttgcttc gaattaaacc acatgctcca 540
ccgcttgtgc gggcccccgt caattccttt gagtttcagt cttgcgaccg tactccccag 600
gcggagtgct taatgcgtta gctgcagcac taaggggcgg aaacccccta acacttagca 660
ctcatcgttt acggcgtgga ctaccagggt atctaatcct gttcgctccc cacgctttcg 720
ctcctcagcg tcagttacag accagagagt cgccttcgcc actggtgttc ctccacatct 780
ctacgcattt caccgctaca cgtggaattc cactctcctc ttctgcactc aagttcccca 840
gtttccaatg accctccccg ggttgagccg ggggctttca catcagactt aagaaaccgc 900
ctgcgagccc tttacgccca ataattccgg acaacgcttg ccacctacgt attaccgcgg 960
ctgctggcac gtagttagcc gtggctttct ggttaggtac cgtcaaggtg ccgccctatt 1020
tgaacggcac ttgttcttcc ctaacaacag agctttacga tccgaaaacc ttcatcactc 1080
acgcggcgtt gctccgtcag actttcgtcc attgcggaag attccctact gctgcctccc 1140
gtaggagtct tgggccgtgt ctcagtccca gtgtggccga tcacccttct caggtcggct 1200
acgcatcgtc gccttggtga gccgttacct caccaactag ctaatgcgcc gcggggtgcc 1260
actctgtgaa gtgggtagcc gaagccacct tttatgtctg aaccatgcgg ttcaaacaac 1320
catccggtat tagccccggt ttcccggagt tatcccagtc ttacaggcag gttacccacg 1380
tgttactcac ccgtccgccg ctaacatcag ggagcaagct cccatctgtc cgctcgactt 1440
gcatgtatag cacccgcact gcc 1463
Claims (5)
1. The bacillus amyloliquefaciens is characterized in that the bacillus amyloliquefaciens is bacillus amyloliquefaciens Bam-6 (Bam-6)Bacillus amyloliquefaciens Bam-6) And has been preserved in China Center for Type Culture Collection (CCTCC) at 8 months and 16 days in 2021, with the preservation number of CCTCC NO: m20211040.
2. Use of the bacillus amyloliquefaciens of claim 1 for killing myzus persicae.
3. A green peach aphid exterminating agent, characterized in that its components comprise a fermentation broth of the Bacillus amyloliquefaciens Bam-6 of claim 1.
4. The aphis persicae extermination preparation according to claim 3, wherein the preparation is in the form of an aqueous preparation, and the components of the preparation further comprise a bacteriostatic agent; the bacteriostatic agent is NaNO 2 、NaNO 3 、NaN 3 And EDTA.
5. Use of the bacillus amyloliquefaciens of claim 1 to inhibit fusarium oxysporum and xanthomonas carpi.
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Citations (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103987260A (en) * | 2011-12-15 | 2014-08-13 | 拜耳知识产权有限责任公司 | Active ingredient combinations having insecticidal and acaricidal properties |
| CN104498386A (en) * | 2014-11-25 | 2015-04-08 | 山西农业大学 | Preparation method and applications of wild jujube endophytic bacillus amyloliquefaciens new strain SZ23 and fermentation broth |
| CN105331557A (en) * | 2015-11-18 | 2016-02-17 | 浙江省农业科学院 | Bacillus amyloliquefaciens HZ179 and application thereof in preparation of microbial insecticide |
| CN105543151A (en) * | 2016-02-23 | 2016-05-04 | 云南省微生物发酵工程研究中心有限公司 | Bacillus amyloliquefaciens strain W1 for controlling plant spider mites and application |
| CN105532735A (en) * | 2015-11-21 | 2016-05-04 | 安徽省农业科学院植物保护与农产品质量安全研究所 | Bactericidal and insecticidal composition containing Bacillus amyloliquefaciens and matrine |
| CN106967752A (en) * | 2017-04-14 | 2017-07-21 | 江南大学 | A kind of method for preparing insecticide through microbial fermentation by raw material of Siberian cocklebur |
| CN106967753A (en) * | 2017-04-14 | 2017-07-21 | 江南大学 | Prince's-feather fermenting plant promoting bacteria and its application in preventing and treating pest and disease damage |
| CN111893076A (en) * | 2020-08-21 | 2020-11-06 | 山东胜伟盐碱地科技有限公司 | Microbial insecticide for preventing and treating aphids and preparation method thereof |
| KR102201525B1 (en) * | 2019-09-06 | 2021-01-12 | (주)현농 | Pest control of the formulations of Bacillus amyloliquefaciens PPL and plant extract against |
Family Cites Families (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| PL3855919T3 (en) * | 2018-09-28 | 2023-06-05 | Fmc Corporation | Bacillus amyloliquefaciens fcc1256 compositions and methods of controlling plant pathogens |
-
2021
- 2021-09-07 CN CN202111040784.7A patent/CN113817634B/en active Active
Patent Citations (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103987260A (en) * | 2011-12-15 | 2014-08-13 | 拜耳知识产权有限责任公司 | Active ingredient combinations having insecticidal and acaricidal properties |
| CN104498386A (en) * | 2014-11-25 | 2015-04-08 | 山西农业大学 | Preparation method and applications of wild jujube endophytic bacillus amyloliquefaciens new strain SZ23 and fermentation broth |
| CN105331557A (en) * | 2015-11-18 | 2016-02-17 | 浙江省农业科学院 | Bacillus amyloliquefaciens HZ179 and application thereof in preparation of microbial insecticide |
| CN105532735A (en) * | 2015-11-21 | 2016-05-04 | 安徽省农业科学院植物保护与农产品质量安全研究所 | Bactericidal and insecticidal composition containing Bacillus amyloliquefaciens and matrine |
| CN105543151A (en) * | 2016-02-23 | 2016-05-04 | 云南省微生物发酵工程研究中心有限公司 | Bacillus amyloliquefaciens strain W1 for controlling plant spider mites and application |
| CN106967752A (en) * | 2017-04-14 | 2017-07-21 | 江南大学 | A kind of method for preparing insecticide through microbial fermentation by raw material of Siberian cocklebur |
| CN106967753A (en) * | 2017-04-14 | 2017-07-21 | 江南大学 | Prince's-feather fermenting plant promoting bacteria and its application in preventing and treating pest and disease damage |
| KR102201525B1 (en) * | 2019-09-06 | 2021-01-12 | (주)현농 | Pest control of the formulations of Bacillus amyloliquefaciens PPL and plant extract against |
| CN111893076A (en) * | 2020-08-21 | 2020-11-06 | 山东胜伟盐碱地科技有限公司 | Microbial insecticide for preventing and treating aphids and preparation method thereof |
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