CN113812407B - A kind of insecticidal composition containing bromfenib and tetrazolium and application thereof - Google Patents

Abstract

The invention relates to the technical field of pesticide insecticides, in particular to an insecticidal composition containing chlorfenapyr fluorobenzene bisamide and tebuconazole and application thereof, wherein the weight ratio of the chlorfenapyr fluorobenzene bisamide to the tebuconazole is 1:2 and 1: 5. The compound composition has obvious synergistic effect on lepidoptera pests and phyllotreta striolata on corns and vegetables, particularly on control of spodoptera frugiperda and plutella xylostella, can reduce the using amount of medicaments, reduce the cost and reduce the harm to the environment. And the pesticide composition has good quick-acting property and long lasting period, can effectively delay the generation of pesticide resistance of pests, and is particularly suitable for controlling resistant pests.

Description

A kind of insecticidal composition containing bromfenib and tetrazolium and application thereof

technical field

The invention relates to the technical field of pesticides, in particular to an insecticidal composition containing bromfenib and tetrazolium and application thereof.

Background technique

Broflanilide, molecular formula: C ₂₅ H ₁₄ F ₁₁ BrN ₂ O ₂ . Bromofenib is a metadiamide insecticide jointly developed by Japan's Mitsui Chemicals and Germany's BASF, and will be registered in my country in 2020. The insecticide is an allosteric modulator of gamma-aminobutyric acid (GABA)-gated chloride channels, causing hyperexcitability and convulsions in insects. Bromfenib can also be used for seed treatment, control of grain nematodes, etc., and can be used in special pest control fields, such as the control of termites, ants, cockroaches, flies, etc.

Tetrazolium is a second-generation Group28 bisamide insecticide developed by Bayer in 2014, and will be registered in my country in 2020. The insecticide belongs to the diamide insecticides of the anthranilamide insecticides. It works by activating the ryanodine receptors in the calcium release channel. It is an anthranilide that acts by ingesting Amide insecticides cause loss of muscle control and paralysis by interfering with the calcium release channel of nitin receptors in insect fish, causing them to quickly stop feeding and eventually kill.

Tetrazolium can be efficiently controlled at low doses for rice, fruit trees, vegetables and other crops, and has a significant control effect on pests such as Diploid spp., Beet armyworm, and thrips. It mainly acts on lepidopteran larvae through stomach poisoning and also has contact killing effect, and mainly produces contact killing effect on adults. The medicament is simple to use and can be used for foliar treatment, soil treatment and seed treatment.

The problem of resistance to pesticide pests is a global problem. In recent years, due to the long-term use of chemical agents and the influence of unscientific use of pesticides, the problem of agricultural pest resistance has become increasingly serious. For pests that develop resistance in agriculture, the most effective method is to develop non-cross-resistance with existing pesticides. However, the development of new pesticides has problems such as high cost, long time and difficulty.

The compounding of pesticides can expand the insecticidal spectrum and delay the resistance of pests. Therefore, research and development of high-efficiency, low-toxicity, and environmentally friendly insecticide compositions have a positive effect on the sustainable development of agriculture.

SUMMARY OF THE INVENTION

The purpose of the present invention is to provide a kind of agricultural compound insecticide, the provided agricultural compound insecticide contains the insecticidal composition with obvious synergistic effect, and has the advantages of less dosage, low cost and good efficacy. , can delay pest resistance and is environmentally friendly.

In a first aspect, the present invention provides a pesticidal composition, the active ingredients of the pesticidal composition comprise bromfenib and tetrazolium, in a weight ratio, the fenfenib and The ratio of tetraconazole is 1:2 or 1:5. In the present invention, it is found that there is no cross-resistance between bromilifenamide and tetrazolium.

According to the understanding of those skilled in the art, the application of the above-mentioned insecticidal composition in controlling lepidopteran pests is claimed; and the application of the above-mentioned insecticidal composition in the preparation of pesticides.

In a second aspect, the present invention provides a pesticide formulation containing the above-mentioned pesticidal composition.

In the pesticide formulation provided by the present invention, the mass percentage of the sum of the weights of bromfenib and tetrazolium in the total weight of the pesticide formulation is 5%-70%.

The pesticide formulation provided by the present invention also includes carriers and adjuvants, the adjuvants include dispersants, emulsifiers, disintegrants, stabilizers, wetting agents, synergists, penetrants, defoaming agents, thickeners, one or more of preservatives, solvents or fillers;

The carrier is one or more of kaolin, diatomite, bentonite, attapulgite, silica, starch or light calcium carbonate;

The pesticide formulation is microemulsion, water emulsion, wettable powder, water dispersible granule, emulsifiable concentrate or suspension.

The pesticide preparation provided by the invention is a bromfenib/tetrazolium suspending agent, calculated in mass percentage and in parts by weight, 4-5 parts by weight of fenfenib, 10-20 parts by weight of tetrazolium parts by weight, 4-6 parts by weight of alkylphenol polyoxyethylene ether formaldehyde condensate sulfate, 3-5 parts by weight of pulling powder, 0.2-0.5 parts by weight of silicone oil, 0.3-0.7 parts by weight of xanthan gum, polyethylene glycol 0.4-0.9 parts by weight, 61.9-78.1 parts by weight of deionized water.

In a third aspect, the present invention provides the preparation method of the above pesticide preparation, characterized in that, according to the formula, dispersing agent, wetting agent, defoaming agent, thickening agent and antifreezing agent are added to the reaction kettle for high-speed grinding, uniformly mixed, and then added The suspension agent of the present invention can be prepared by grinding the original medicines of bromfenib and tetrazolium in a ball mill for 2-4 hours so that the particle diameters of the particles are all below 5 μm.

Another object of the present invention is the use of the above-mentioned pesticidal composition or pesticide formulation for controlling agricultural pests. It is preferably used for the control of lepidopteran pests and flea beetles on corn and vegetables, especially for the control of Spodoptera frugiperda and diamondback moth.

Therefore, the present invention also claims to protect the application of the above-mentioned insecticidal composition or the above-mentioned pesticide formulation in controlling lepidopteran pests and flea beetle.

In a fourth aspect, the present invention provides a method for controlling lepidopteran pests, using the above-mentioned pesticide formulation to be applied to plants when the pests occur.

The beneficial effects of the insecticidal composition of the present invention are:

(1) The combination of two active ingredients in the insecticidal composition provided by the present invention has obvious synergistic effect, which is not a simple superposition of the activities of each component, but has a significant synergistic effect, which improves the control effect and reduces the The dosage of active ingredients has good control effect on Spodoptera frugiperda and diamondback moth;

(2) The pesticide preparation provided by the present invention can effectively delay the generation of pesticide resistance of pests, prolong the service life of the pesticide, and can effectively delay the generation of pesticide resistance of pests by compounding compounds with different action mechanisms, with specific auxiliaries and carriers, and adopting a specific preparation method. Integrated pest management is of great significance;

(3) The pesticide formulation provided by the present invention reduces the dosage, thereby reducing the cost and reducing the pollution to the environment, and has good safety, which meets the safety requirements of the pesticide formulation.

Detailed ways

The following examples are intended to illustrate the present invention, but not to limit the scope of the present invention. Modifications or substitutions made to the methods, steps or conditions of the present invention without departing from the spirit and essence of the present invention all belong to the protection scope of the present invention.

Unless otherwise specified, the experimental materials, reagents, instruments, etc. used in the examples of the present invention are all commercially available; if not specified, all the technical means in the embodiments of the present invention are conventional means known to those skilled in the art .

In the embodiment of the present invention, the used fenflubendiamide and tetrazolium are conventional commercially available technical drugs, and the manufacturer of fenflubendiamide is Japan's Mitsui Chemicals AGRO Co., Ltd., registration certificate number: PD20200656; tetrazolium The manufacturer of insectamide is Bayer AG, registration number: PD20200655. The proportions or percentages in the embodiments of the present invention are all mass ratios. Embodiment 1 The combined virulence of different ratios of bromflubendiamide and tetrazolium to Spodoptera frugiperda

This example provides a combined virulence test of bromfenib and tetrazolium. The present embodiment adopts the leaf soaking method, and takes Spodoptera frugiperda as the test object, and the concrete method is as follows:

Refer to "NY/T 1154.14-2008 Pesticide Indoor Bioassay Test Guidelines for Pesticide Part 14: Leaf Immersion Method" for bioassay.

The steps are as follows: the original medicinal acetone is prepared into a mother solution, and then the mother solution is diluted to 5-7 serial concentrations with 0.1% Triton X-100 aqueous solution in equal proportions, and the non-transgenic fresh corn leaves at the flare stage are cut into 1cm× 1cm (length x width) leaf segment. The leaf segments were soaked in different concentrations of medicinal solution for 20 s, dried on filter paper, and placed in a 12-well culture plate. Each well of the culture plate was pre-poured with 1 mL of 1.5% agar for moisturizing. There were 6 leaf segments in each hole, and a 3rd instar Spodoptera frugiperda larva was inserted. Each treatment was replicated 3 times with no less than 12 larvae per replicate. The control was treated with 0.1% Triton X-100 aqueous solution. 72h check result. The larvae were considered dead if they did not respond to light touch with a brush.

Data were statistically analyzed using POLO software (LeOra Software Inc., California, USA), with control mortality less than 10% as a valid measure and corrected for control mortality. The Slope value of the virulence regression line and its standard error SE, LC50 value and P value were obtained.

The co-toxicity coefficient (CTC value) of the mixture was calculated by Sun Yunpei method.

The percentage content of medicament A in the mixture = the content of medicament A/(the content of medicament A + the content of medicament B) × 100%.

The actual toxicity index (ATI) of the mixture = LC50 of the A agent/LC50 of the mixture × 100.

Mixture theoretical toxicity index (TFI)=ATI(A)×percentage of agent A in the mixture+ATI(B)×percentage of agent B in the mixture.

Co-toxicity coefficient (CTC)=actual toxicity index ATI of the mixture/theoretical toxicity index TFI×100 of the mixture.

Co-toxicity coefficient ≥ 120 indicates synergistic effect; co-toxicity coefficient ≤ 80 indicates antagonistic effect; co-toxicity coefficient between 80-120 indicates additive effect.

Toxicity test results are shown in Table 1:

Table 1 Indoor virulence assay results of bromflubendiamide and tetrazolium to Spodoptera frugiperda larvae

As shown in Table 1, compared with the single dose, when the weight ratio of bromfenib and tetrazolium in the insecticidal composition is 1:2 and 1:5, the co-toxicity coefficient is greater than 120, showing obvious The synergistic effect showed that the composition had excellent insecticidal activity against Spodoptera frugiperda.

Example 2 Determination of field control effect of compound pesticide formulation on Spodoptera frugiperda

In this embodiment, the pesticide formulations used are (1) 5% bromfenib suspending agent, (2) 200g/L tetrazolium suspending agent (3) 15% brofenfenib/tetrazolium Amide suspending agent, (4) 24% bromilifenib/tetrazolium suspending agent.

The formula of 15% fenflubenzamide/tetrazolium suspending agent is: based on the total weight of the suspending agent, it includes: 5% fenflubenzamide, 10% tetrazolium, alkylphenol polyamide Oxyethylene ether formaldehyde condensate sulfate 4%, pull-open powder 3%, silicone oil 0.2%, xanthan gum 0.3%, polyethylene glycol 0.4%, deionized water to make up the balance, to obtain 15% bromfenac Amide/tetrazolium suspension.

The formulation of 24% fenflubendiamide/tetrazolium suspending agent is: based on the total weight of the suspending agent, it includes: fenfenib 4%/tetrazolium 20%/alkylphenol poly Oxyethylene ether formaldehyde condensate sulfate 6%, pull-open powder 5%, silicone oil 0.5%, xanthan gum 0.7%, polyethylene glycol 0.9%, deionized water to make up the balance, to obtain 24% bromfenprodil Amide/tetrazolium suspension.

The method for preparing the above-mentioned 15% bromfenib/tetrazolium suspending agent and 24% bromilifenib/tetrazolium suspending agent is as follows: dispersing agent, wetting agent and defoaming agent are sequentially combined according to the formula. , thickener and antifreeze are added to the reaction kettle for high-speed grinding, mixed evenly, then add bromfenib and tetrazolium, and grind in a ball mill for 2-4 hours, so that the particle size is all below 5μm, The suspending agent of the present invention can be prepared.

In this embodiment, the test object is: Spodoptera frugiperda. The test method for the determination of the control effect is as follows:

(1) Spodoptera frugiperda was moderately infested when the pesticide was applied, the population density was moderate, and the insect age was mostly young, which could meet the requirements of field efficacy tests.

(2) The experiment was arranged in random blocks, with protective rows set up around them. Each treatment was repeated 4 times, and a clear water control was set, and each plot was 60 m ² . Before spraying, the base of insect population was investigated. Five-point sampling method was adopted, 10 plants were continuously surveyed at each point, and a total of 50 corn plants were surveyed in each plot, and were listed and marked. The 3WBD-20 type knapsack electric sprayer was used for uniform spraying, and the water consumption of 50kg per 667m ² for each chemical treatment was uniformly sprayed on each plot. No other chemical was used during the test. The number of insect populations was investigated at 1, 3, 7, and 14 days after spraying, and the reduction rate of the insect population and the corrected control effect were calculated. The results are shown in Table 2.

Insect population decline rate (%)=(Insect population base number before treatment - surviving insect number after treatment)/Insect population base number before treatment × 100;

Control effect (%)=(insect population reduction rate of treatment area-insect population reduction rate of control area)/(100-insect population reduction rate of control area)×100.

Table 2 Field control effect of compound pesticide formulations on Spodoptera frugiperda

It can be seen from Table 2 that the field control effect of each formulation on Spodoptera frugiperda increases with the increase of the dose, and at the same test dose, the control effect of the mixture on Spodoptera frugiperda is significantly better than that of fenflubenzuron The single dose of amide and tetrazolium has excellent quick-acting and long-lasting effects. Although bromfenib and tetrazolium are both bisamides, they have different mechanisms of action. Mixed use can improve the control effect, reduce the dosage, and delay the generation of pest resistance.

Example 3 Determination of field control effect of compound pesticide formulation on diamondback moth in broccoli

The pesticide formulation used in this example is the same as that in Example 2. In this embodiment, the experimental object is diamondback moth. Test method for control effect determination: The row spacing of broccoli cultivation plants is 40cm×60cm, and during the test period, the broccoli diamondback moth is applied at the initial peak period of the young larvae (1 to 2 instars).

The experiment was arranged in random blocks with guard rows around. Each treatment was repeated 4 times, and a clear water control was set, and each plot was 30 m ² . Before spraying, the base of insect population was investigated, and the five-point sampling method was adopted. Each sampling point was investigated for three adjacent broccoli plants, and a fixed-point and fixed-plant survey was carried out. The 3WBD-20 type knapsack electric sprayer was used for uniform spraying, and the water consumption of 30kg per 667m ² for each chemical treatment was uniformly sprayed on each plot. No other chemical was used during the test. The number of insect populations was investigated at 1, 3, 7, and 14 days after spraying, and the reduction rate of the insect population and the corrected control effect were calculated. The results are shown in Table 3.

Insect population decline rate (%)=(Insect population base number before treatment - surviving insect number after treatment)/Insect population base number before treatment × 100;

Control effect (%)=(insect population reduction rate of treatment area-insect population reduction rate of control area)/(100-insect population reduction rate of control area)×100.

Table 3 Field control effects of compound pesticide formulations against diamondback moth in broccoli

It can be seen from Table 3 that the field control effect of each formulation on diamondback moth increases with the increase of the dose, and at the same test dose, the control effect of the mixture on diamondback moth is significantly better than that of bromfenprod and tetrafluorobenzamide. The single dose of tolafenamide has excellent quick-acting and long-lasting effects. Since bromfenib and tetrazolium have different mechanisms of action, mixed use can improve the control effect, reduce the dosage, and delay the generation of pest resistance.

The control effect of embodiment 4 compound pesticide preparation on other pests

The pesticide formulation used in this example is the same as that in Example 2. The experimental object of the present embodiment is cabbage yellow curved stripe jumping beetle.

Vegetables are grown all year round in the experimental site, and flea beetles occur frequently throughout the year. The test method for measuring the control effect is the same as that in Example 3, and the control effect on adults is mainly investigated, and the results are shown in Table 4.

Table 4 Determination of field control effect of compound pesticide formulations on Flea beetle in cabbage

It can be seen from Table 4 that the field control effect of each preparation on Flea beetle from Brassica oleracea increases with the increase of the dose, and at the same application dose, the control effect of the mixture on F. The single dose of bromfenib and tetrazolium has better quick-acting and long-lasting effects. Since bromfenib and tetrazolium have different mechanisms of action, mixed use can improve the control effect, reduce the dosage, and delay the generation of pest resistance.

To sum up, the present invention adopts effective doses of two active ingredients, bromilifenamide and tetrazolium, and the obtained pesticide composition has a significant synergistic effect. The control effect of Lepidoptera pests, especially against Spodoptera frugiperda and diamondback moth, is obviously improved, and it also has good control effect against other pests such as flea beetle. The pesticidal composition of the invention has the advantages of less dosage per unit, good quick-acting effect, long lasting effect and the like, is safe for the environment and crops, and has great potential for popularization and use in agricultural production.

Although the present invention has been described in detail above with general description and specific embodiments, it is obvious to those skilled in the art that some modifications or improvements can be made on the basis of the present invention. Therefore, these modifications or improvements made without departing from the spirit of the present invention fall within the scope of the claimed protection of the present invention.

Claims (10)

1. The insecticidal composition is characterized in that active ingredients of the insecticidal composition comprise flubendiamide and tebuconazole, and the ratio of the flubendiamide to the tebuconazole is 1:2 or 1:5 in terms of weight ratio.

2. Use of the pesticidal composition of claim 1 for controlling lepidopteran pests.

3. Use of the pesticidal composition according to claim 1 for the preparation of a pesticide.

4. A pesticidal formulation comprising the pesticidal composition of claim 1.

5. The pesticide preparation as claimed in claim 4, wherein the sum of the weight of the flubendiamide and the tebuconazole accounts for 5-70% of the total weight of the pesticide preparation.

6. The pesticide preparation as claimed in claim 5, further comprising an auxiliary agent and a carrier, wherein the auxiliary agent is one or more of a dispersing agent, an emulsifier, a disintegrating agent, a stabilizer, a wetting agent, a synergist, a penetrating agent, a defoaming agent, a thickening agent, a preservative, a solvent or a filler;

the carrier is one or more of kaolin, diatomite, bentonite, attapulgite, white carbon black, starch or light calcium carbonate;

the pesticide preparation is microemulsion, aqueous emulsion, wettable powder, water dispersible granules, missible oil or suspending agent.

7. The pesticide preparation of claim 6, which is characterized by comprising, by weight, 4-5 parts of the brofenpyrad/tebuconazole amide suspending agent, 10-20 parts of the tebuconazole amide, 4-6 parts of alkylphenol polyoxyethylene formaldehyde condensate sulfate, 3-5 parts of nekal, 0.2-0.5 part of silicone oil, 0.3-0.7 part of xanthan gum, 0.4-0.9 part of polyethylene glycol and 61.9-78.1 parts of deionized water.

8. The preparation method of the pesticide preparation as claimed in claim 6, characterized in that the suspending agent is prepared by adding the dispersant, the wetting agent, the defoamer, the thickener and the antifreeze into a reaction kettle according to the formula in sequence, grinding at high speed, mixing uniformly, then adding the technical grade of the brobenbenbenbendiamide and the tebuconazole, and grinding in a ball mill for 2-4h to ensure that the particle size of the particles is below 5 μm.

9. Use of the pesticidal composition of claim 1 or the pesticidal formulation of any one of claims 4 to 7 for controlling spodoptera frugiperda, plutella xylostella or phyllotreta striolata on vegetables.

10. A method for controlling lepidopteran pests, characterized in that a pesticide formulation according to any one of claims 4 to 7 is applied to a plant at the time of pest infestation.