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CN113801218A - Reconstructed collagen with molecular weight having polydispersion characteristic and application thereof - Google Patents

Reconstructed collagen with molecular weight having polydispersion characteristic and application thereof Download PDF

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CN113801218A
CN113801218A CN202111231605.8A CN202111231605A CN113801218A CN 113801218 A CN113801218 A CN 113801218A CN 202111231605 A CN202111231605 A CN 202111231605A CN 113801218 A CN113801218 A CN 113801218A
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collagen
molecular weight
skin
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polydispersity
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蒋波
李霞
王宁馨
罗振宇
郭纪彤
尹越
朱凌旋
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Sichuan University
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    • A61K8/30—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds
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Abstract

本发明公开了一种分子量具有多分散特性的重建胶原蛋白及其用途，所述重建胶原蛋白分子结构具有三螺旋构象，圆二色谱分析在波长220±10nm处有正峰；分子量分布呈多分散特性，其相对分子质量范围为1KD～300KD；本发明公开的重建胶原蛋白结构保留了典型胶原蛋白分子的生物活性，既具有高分子量胶原蛋白保湿、美白、营养、修复等特性，又赋予低分子量特有溶解性及渗透性等新功能，实验发现能渗透皮肤组织，重建胶原蛋白可应用于美容和皮肤修复领域。

Figure 202111231605

The invention discloses a reconstructed collagen with polydispersity in molecular weight and use thereof. The reconstructed collagen molecular structure has a triple helix conformation, and circular dichroism analysis has a positive peak at a wavelength of 220±10 nm; the molecular weight distribution is polydisperse Its relative molecular mass ranges from 1KD to 300KD; the reconstructed collagen structure disclosed in the present invention retains the biological activity of typical collagen molecules, and not only has the characteristics of moisturizing, whitening, nutrition and repairing of high molecular weight collagen, but also gives low molecular weight collagen. It has unique new functions such as solubility and permeability. It has been found in experiments that it can penetrate the skin tissue and rebuild collagen, which can be used in the fields of beauty and skin repair.

Figure 202111231605

Description

Reconstructed collagen with molecular weight having polydispersion characteristic and application thereof
Technical Field
The invention relates to the technical field of medical cosmetology, in particular to a reconstructed collagen with polydisperse molecular weight, a composition and application thereof.
Background
The skin is the largest organ of the human body, and the stratum corneum of the skin covers the living body and keeps moisture and protects against the invasion of foreign substances. The natural barrier of the skin refers to a brick wall structure consisting of stratum corneum cells and lipid and natural moisturizing factor between the cells, and the surface of the brick wall structure is attached with a skin lipid membrane, thereby forming a natural protective barrier for the human body together. The skin barrier not only prevents pathogen invasion, but also integrates the mediation of the stratum corneum, keratinocyte structural components, and immune cell antigens. The barrier function of the skin has bidirectional property, on one hand, various organs and tissues in the body are protected from being invaded by harmful factors of machinery, physics, chemistry and biology in the external environment to cause diseases, on the other hand, various nutrient substances, moisture, electrolytes and other substances in the body are prevented from being lost, the moisture content of the skin is maintained, and the skin is moistened. Substances in the external environment are difficult to pass through the skin due to the barrier action of the skin.
Collagen is a major component of extracellular matrix of animal connective tissue, and is present in various tissues and organs, such as animal skin, bones, tendons, pericardium, and the like. Collagen is an extracellular structural protein, which not only provides a site for nutrition metabolism for cells, but also influences the morphological structure, cell movement and growth metabolism of cells. The molecular structure of collagen is characterized by having a triple-helix structure, and 3 left-handed helical chains are mutually wound and twisted into a tight right-handed helical structure, and the molecules of the collagen are very stable. More than 20 different types of collagen have been discovered to date. The type I collagen is the collagen with the largest content in animals, and is also most widely applied in the field of tissue engineering. The amino acid composition of different types of collagen is approximately the same, differing only by source and type. The continuous development of molecular biology has led to the gradual recognition of the good physical properties and biological characteristics of collagen, and the collagen is widely used in the fields of biomedicine, materials, chemical industry, food, agriculture, and the like.
The molecular weight and its distribution directly influence the biological effect of biopolymers. Macromolecular collagen molecules can be reconstructed into collagen fibers in vitro, so that the collagen fibers have good cell compatibility and physiological functions of promoting platelet aggregation and the like; the gelatin has a slightly smaller molecular weight, can partially reconstruct the triple-helix structure of collagen molecules, and can be used for hemostasis; the collagen peptide with smaller molecular weight is complex in composition, and some special polypeptides play different roles. Literature research also finds that the collagen polypeptide with higher molecular weight shows the functions of stopping bleeding, promoting blood coagulation and promoting trauma healing in mouse animal experiments; and the collagen polypeptide with lower molecular weight has obvious promotion effect on scald repair. Therefore, the molecular weight and the distribution of the collagen are important related to the physiological functions of the collagen. Therefore, it is urgently needed to develop a reconstructed collagen having bioactivity of collagen and good water solubility and permeability aiming at the characteristics of skin and biopolymer.
Disclosure of Invention
The invention aims to overcome the defects of the prior art and provide a reconstructed collagen with triple helix conformation and polydisperse molecular weight distribution;
another object of the present invention is to provide the use of such reconstituted collagen, which can be applied in the fields of cosmetology and skin repair.
The purpose of the invention is realized by the following technical scheme: a reconstituted collagen with a molecular weight having polydisperse characteristics, the reconstituted collagen molecular structure having a triple helix conformation, circular dichroism analysis having a positive peak at a wavelength of 220 ± 10 nm; the molecular weight distribution is polydisperse, and the relative molecular weight range is 1 KD-300 KD.
Further, the reconstituted collagen is prepared from mammalian tissue. The reconstructed collagen of the invention is obtained by animal tissues through a series of biochemical reactions and unit processes.
Preferably, the animal tissue is skin, tendon, bone, pericardium or peritoneum.
Further, the content of the reconstructed collagen protein is not less than 90 g/100 g.
Further, the content of hydroxyproline in the reconstructed collagen is not less than 3.0 g/100 g.
Further, the complete dissolution time of the reconstructed collagen in water with the temperature of 37 +/-1 ℃ is not more than 10 min.
A composition comprising the reconstituted collagen described above.
The reconstructed collagen of the invention is applied to the fields of beauty treatment and skin repair.
Further, the reconstituted collagen is able to penetrate skin tissue.
Further, cosmetic products including skin moisturizers, skin nourishments, or skin whiteners, and skin repair products are included.
The cosmetic product and the skin repair product of the invention contain cosmetically acceptable auxiliary materials besides the reconstructed collagen of the invention, and the products can be cream, spray, facial mask, gel, lotion, emulsion and the like.
The invention has the following advantages: the invention discloses a reconstructed collagen, which not only retains the triple-helix conformation of typical collagen, but also has the characteristic of polydisperse distribution of molecular weight.
Drawings
FIG. 1 is a circular dichroism diagram of collagen, wherein A is typical macromolecular collagen, and B is reconstructed collagen according to the present invention.
FIG. 2 is a graph showing the distribution of the relative molecular weight ranges of the reconstituted collagen according to the present invention.
FIG. 3 is a two-photon confocal micrograph of the skin permeability of a mouse with reconstructed collagen, wherein FIGS. 3-1 and 3-2 are a negative control skin single-layer image and a full-layer image, respectively; FIGS. 3-3, 3-4 are positive control skin single-layer images and full-layer images, respectively; FIGS. 3-5 and 3-6 are the skin single-layer image and the full-layer image of the commercial recombinant collagen polypeptide reference sample, respectively; 3-7, 3-8 are respectively single-layer images and full-layer images of the skin of the reconstructed collagen smeared for 1h according to the invention; FIGS. 3-9 and 3-10 are single-layer images and full-layer images of the skin of 2h after the reconstructed collagen is smeared.
Detailed Description
The invention is further described with reference to the following figures and examples, without limiting the scope of the invention to the following:
example 1:
a reconstructed collagen with a three-spiral structure and molecular weight and polydisperse characteristic is prepared by adopting mammalian skin meeting the requirements of regulations through a series of biochemical reactions and unit processes, the main component is the collagen, the protein content is 91(g/100g), the hydroxyproline content is 3.5(g/100g), the relative molecular mass range is 1 KD-300 KD, a positive peak exists at the wavelength of 220 +/-10 nm by circular dichroism detection, and the complete dissolution time in water at 37 +/-1 ℃ is not more than 10 min.
Example 2:
a reconstructed collagen with a three-spiral structure and a molecular weight and a polydisperse characteristic is extracted from mammalian bones meeting the requirements of regulations through a series of biochemical reactions and unit processes, the main component is the collagen, the protein content is 93(g/100g), the hydroxyproline content is 4.1(g/100g), the relative molecular mass range is 6 KD-250 KD, a positive peak exists at the wavelength of 220 +/-10 nm by circular dichroism detection, and the complete dissolution time in water at 37 +/-1 ℃ is not more than 10 min.
Example 3:
a reconstructed collagen with a three-spiral structure and a molecular weight and polydispersion characteristic is extracted by a series of biochemical reactions and unit processes from mammal pericardium meeting the requirements of regulations, and the main component of the reconstructed collagen is collagen. The protein content is 95(g/100g), the hydroxyproline content is 3.8(g/100g), the relative molecular mass range is 1 KD-200 KD, the circular dichroism chromatogram detection has a positive peak at the wavelength of 220 +/-10 nm, and the complete dissolution time in water at 37 +/-1 ℃ is not more than 10 min.
Example 4:
a reconstructed collagen with a three-helix structure and a molecular weight and polydispersion characteristic is extracted by a series of biochemical reactions and unit processes from peritoneum of mammals meeting the requirements of regulations, and the main component of the reconstructed collagen is collagen. The protein content is 97(g/100g), the hydroxyproline content is 5.3(g/100g), the relative molecular mass range is 6 KD-180 KD, the circular dichroism chromatogram detection has a positive peak at the wavelength of 220 +/-10 nm, and the complete dissolution time in water at 37 +/-1 ℃ is not more than 10 min.
Example 5:
a reconstructed collagen with a three-helix structure and a molecular weight and polydispersion characteristic is extracted from mammalian skin meeting the requirements of regulations through a series of biochemical reactions and unit processes, and the main component of the reconstructed collagen is collagen. The protein content is 95(g/100g), the hydroxyproline content is 5.7(g/100g), the relative molecular mass range is 3 KD-150 KD, the circular dichroism chromatogram detection has a positive peak at the wavelength of 220 +/-10 nm, and the complete dissolution time in water at 37 +/-1 ℃ is not more than 10 min.
Example 6:
a composition comprising 0.1-99% of the reconstituted collagen prepared in example 1.
Example 7:
a cosmetic preparation in the form of cream contains the reconstituted collagen prepared in example 2 in an amount of 20%, and further contains a moisturizing ingredient and cosmetically acceptable adjuvants.
Example 8:
a cosmetic product, in the form of a spray, comprises the reconstituted collagen prepared in example 3 in an amount of 18%, and further comprises skin nutrients and cosmetically acceptable adjuvants.
Example 9:
a cosmetic product is a facial mask, which contains 55% of the reconstituted collagen prepared in example 4, and further contains whitening components and cosmetically acceptable auxiliary materials.
Example 10:
a cosmetic preparation in the form of gel comprises the reconstituted collagen prepared in example 5 in an amount of 70%, and further comprises a moisturizing ingredient and cosmetically acceptable adjuvants.
Example 11:
a cosmetic product, a lotion, contains the reconstituted collagen prepared in example 2 in an amount of 38%, and further contains skin nutrients and cosmetically acceptable adjuvants.
Example 12:
a cosmetic preparation, in the form of an emulsion, comprises 85% of the reconstituted collagen prepared in example 4, and further comprises a skin-repairing ingredient and cosmetically acceptable adjuvants.
Example 13:
a cosmetic preparation, in the form of a spray, comprises 2% of the reconstituted collagen prepared in example 1, and further comprises a skin moisturizing ingredient and cosmetically acceptable adjuvants.
Example 14:
a skin repair product comprises the reconstructed collagen prepared in example 5 in an amount of 99%, and further comprises a skin repair ingredient and cosmetically acceptable excipients.
The following experiments illustrate the beneficial effects of the present invention:
1. circular dichroism spectrum experiment
Subject: a-typical macromolecular collagen, B-reconstituted collagen prepared according to example 1 of the invention;
the A-typical macromolecular collagen is prepared by the following method:
1. pretreatment of newborn calf skin
Scraping hair and hair follicle of fresh newborn calf skin, removing tissue and fat, cutting, adding 2000ml mixed solution of alcohol and alkyl halide in volume ratio of 0.5 into 200g of cut skin, soaking for degreasing, replacing the solution every 5 hours, and pouring off the solution after 15 hours; soaking the raw materials in 2000ml of 98% ethanol water solution, replacing the raw materials once in 5 hours, and pouring the raw materials out in 10 hours; washing once by 2000ml of 20 percent ethanol water solution; soaking the fabric in 0.1mol/L EDTA solution with pH of 10.5-11.5 at 4 deg.c for 6 hr, and washing with 2000ml ultrapure water for 3 times; then 2000ml of NaCl aqueous solution with the concentration of 10% is used for soaking, the replacement is carried out once in 4 hours, the solution is poured out in 8 hours, 2000ml of ultrapure water is used for cleaning for 2 times, and the cowhide is placed into 2000ml of ultrapure water to be soaked overnight, and the water is poured out.
2. Enzymolysis with pepsin
Adding 2000ml of 0.4mol/L acetic acid aqueous solution into the above solid substance, adding 0.1g of pepsin with a wet cow hide/pepsin mass ratio of 2000, placing in a refrigerator at 4 deg.C for 5 days, shaking 2 times per day, adjusting pH of the solution to 8-8.5 with alkali solution, standing overnight, adjusting pH of the solution to 2-2.5 with acid solution, standing overnight, centrifuging, collecting supernatant, and removing skin residue.
3. Salting out
Adding solid NaCl to the clear liquid to 15%, placing in a refrigerator at 4 deg.C overnight after adding, centrifuging to obtain solid substance, placing in a dialysis bag, dialyzing with deionized water, desalting, or adding Na with concentration of 0.01mol/L to the clear liquid2HPO4And fully dialyzing 2000ml of the solution for 72 hours, replacing once for 24 hours, centrifugally collecting solid matters in the dialysis bag after 72 hours, dialyzing 2000ml of acetic acid aqueous solution with the concentration of 0.1mol/L, dissolving, dialyzing with deionized water, and desalting to prepare the typical macromolecular collagen.
An experimental instrument: a circular dichroism instrument is adopted;
the experimental results are as follows: the circular dichroism graphs of the two proteins are shown in FIG. 1, and from FIG. 1, the circular dichroism graphs show that the two collagens have stronger negative peaks at about 198nm and weaker positive peaks at about 220nm, which are typical collagen circular dichroism peaks.
Gel permeation chromatography experiments
Subject: reconstituted collagen prepared in example 1;
the experimental method comprises the following steps: using mobile phase 0.1N NaN3 +0.06%NaN3An aqueous solution; standard samples narrow distribution polyethylene glycol (PEO); the flow rate of the mobile phase is 0.6 mL/min; the column temperature is 35 ℃; the method adopts narrow distribution PEO to make a standard curve relative correction method;
the experimental results are as follows: the distribution diagram of the relative molecular weight range of the reconstructed collagen of the invention is shown in figure 2, wherein the relative molecular weight range is 1 KD-300 KD.
Skin penetration test:
the weight of the mouse is 20 +/-2 g, and after the back hair is shaved, the hair is removed by depilatory cream; the reconstructed collagen solution which is prepared by the fluorescence labeling in the embodiment 1 of the invention is evenly smeared on the back skin tissue of the exposed mouse and is protected from light, the whole layer of skin tissue of 2cm multiplied by 2cm is taken down after the skin naturally absorbs for 1h and 2h under the room temperature condition, and is frozen by liquid nitrogen; the tissue is unfrozen and then placed under a two-photon scanning confocal microscope for image acquisition, the tissue is scanned in a full-layer z-axis manner, the step is carried out by 5 microns, and the condition of skin permeation of the mouse with the reconstructed collagen solution is observed. The tissue collagen SHG (second harmonic) imaging excitation wavelength is 950nm, a red mark is adopted, the reconstructed collagen two-photon fluorescence excitation wavelength is 750nm, and a blue mark is adopted. A control group (commercial recombinant collagen polypeptide, molecular weight 55 kDa), a negative control (PBS solution) and a positive control (fluorescent dye PBS dilution) were also set.
The experimental results are shown in FIG. 3, in which FIGS. 3-1 and 3-2 are respectively a negative control skin single-layer image and a full-layer image; FIGS. 3-3, 3-4 are positive control skin single-layer images and full-layer images, respectively; FIGS. 3-5 and 3-6 are the skin single-layer image and the full-layer image of the commercial recombinant collagen polypeptide reference sample, respectively; 3-7, 3-8 are respectively single-layer images and full-layer images of the skin of the reconstructed collagen smeared for 1h according to the invention; FIGS. 3-9 and 3-10 are single-layer images and full-layer images of the skin of 2h after the reconstructed collagen is smeared. As can be seen from fig. 3:
(1) the negative control only detects a red signal of the tissue collagen, the coated PBS solution does not detect an interference signal, and the skin accessories do not detect an interference signal, which indicates that the 750nm excitation detection two-photon fluorescence signal is not interfered;
(2) the positive control detects a blue fluorescence signal which is in the same layer with the tissue collagen red signal under the excitation light of 750nm, which indicates that the fluorescent dye PBS diluent as a positive control can enter the skin dermal collagen layer;
(3) the control sample had entered the collagen layer in the dermis of the skin after 1 hour of application, and only a small amount of the control sample floated on the epidermis layer;
(4) after the reconstructed collagen is smeared for 1 hour, only a small amount of the reconstructed collagen enters a collagen layer in the dermis of the skin, and most of the reconstructed collagen floats on the epidermis; after 2 hours of application, the collagen layer in the dermis of the skin gradually increased and partially floated on the epidermis. It is shown that the low molecular weight fraction of the collagen of the present invention is able to penetrate into the dermis layer of the skin, while the high molecular weight fraction remains on the skin surface after 2 hours of application.
The above description is only for the preferred embodiment of the present invention, but the scope of the present invention is not limited thereto, and any person skilled in the art can substitute or change the technical solution of the present invention and the inventive concept within the technical scope of the present invention.

Claims (10)

1.一种分子量具有多分散特性的重建胶原蛋白，其特征在于，所述重建胶原蛋白分子结构具有三螺旋构象，圆二色谱分析在波长220±10nm处有正峰；分子量分布呈多分散特性，其相对分子质量范围为1KD～300KD。1. the reconstructed collagen with polydispersity in molecular weight, is characterized in that, described reconstructed collagen molecular structure has triple helical conformation, and circular dichroism analysis has a positive peak at wavelength 220±10nm; molecular weight distribution is polydispersity , and its relative molecular mass ranges from 1KD to 300KD. 2.根据权利要求1所述的一种分子量具有多分散特性的重建胶原蛋白，其特征在于，所述重建胶原蛋白由哺乳动物组织制备而成。2 . The reconstituted collagen with polydispersity in molecular weight according to claim 1 , wherein the reconstituted collagen is prepared from mammalian tissue. 3 . 3.根据权利要求2所述的一种分子量具有多分散特性的重建胶原蛋白，其特征在于，所述的动物组织为皮肤、肌腱、骨骼、心包或腹膜。3 . The reconstructed collagen with polydispersity in molecular weight according to claim 2 , wherein the animal tissue is skin, tendon, bone, pericardium or peritoneum. 4 . 4.根据权利要求1所述的一种分子量具有多分散特性的重建胶原蛋白，其特征在于，所述重建胶原蛋白蛋白质含量不小于90 g/100g。4 . The reconstructed collagen with polydispersity in molecular weight according to claim 1 , wherein the protein content of the reconstructed collagen is not less than 90 g/100 g. 5 . 5.根据权利要求1所述的一种分子量具有多分散特性的重建胶原蛋白，其特征在于，所述重建胶原蛋白中羟脯氨酸含量不小于3.0 g/100g。5 . The reconstructed collagen with polydispersity in molecular weight according to claim 1 , wherein the content of hydroxyproline in the reconstructed collagen is not less than 3.0 g/100 g. 6 . 6.根据权利要求1所述的一种分子量具有多分散特性的重建胶原蛋白，其特征在于，所述重建胶原蛋白在37℃±1℃的水中完全溶解时间不大于10min。6 . The reconstituted collagen with polydispersity in molecular weight according to claim 1 , wherein the reconstituted collagen is completely dissolved in water at 37° C.±1° C. for no more than 10 minutes. 7 . 7.一种组合物，其特征在于，包含权利要求1-6中任一项所述的重建胶原蛋白。7. A composition comprising the reconstituted collagen of any one of claims 1-6. 8.根据权利要求1-6中任一项所述的重建胶原蛋白在美容和皮肤修复领域中的应用。8. The use of the reconstructed collagen according to any one of claims 1-6 in the field of beauty and skin repair. 9.根据权利要求8所述的应用，其特征在于，所述重建胶原蛋白能渗透皮肤组织。9. The use according to claim 8, wherein the reconstructed collagen can penetrate skin tissue. 10.根据权利要求8所述的应用，其特征在于，包括美容制品和皮肤修复制品，所述美容制品包括皮肤保湿品、皮肤营养品或皮肤美白品。10. The application according to claim 8, characterized in that it includes cosmetic products and skin repair products, and the cosmetic products include skin moisturizing products, skin nourishing products or skin whitening products.
CN202111231605.8A 2021-10-22 2021-10-22 Reconstructed collagen with molecular weight having polydispersion characteristic and application thereof Pending CN113801218A (en)

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余小月 等: "胶原蛋白的结构和消化吸收特性及营养价值评价进展", 食品工业科技 *

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