CN113782413B - Efficient sample injection system for single cell detection and use method - Google Patents
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- CN113782413B CN113782413B CN202111126323.1A CN202111126323A CN113782413B CN 113782413 B CN113782413 B CN 113782413B CN 202111126323 A CN202111126323 A CN 202111126323A CN 113782413 B CN113782413 B CN 113782413B
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- 238000001514 detection method Methods 0.000 title claims abstract description 31
- 238000002347 injection Methods 0.000 title claims abstract description 27
- 239000007924 injection Substances 0.000 title claims abstract description 27
- 238000000034 method Methods 0.000 title claims description 14
- 239000002245 particle Substances 0.000 claims abstract description 18
- 239000000443 aerosol Substances 0.000 claims abstract description 9
- 239000007789 gas Substances 0.000 claims description 60
- 239000012159 carrier gas Substances 0.000 claims description 32
- 238000000889 atomisation Methods 0.000 claims description 30
- 239000006285 cell suspension Substances 0.000 claims description 17
- 238000005086 pumping Methods 0.000 claims description 16
- 230000009471 action Effects 0.000 claims description 12
- 230000002093 peripheral effect Effects 0.000 claims description 10
- 230000001133 acceleration Effects 0.000 claims description 8
- 238000001819 mass spectrum Methods 0.000 claims description 5
- 238000009434 installation Methods 0.000 claims description 4
- 230000035945 sensitivity Effects 0.000 abstract description 11
- 238000001816 cooling Methods 0.000 abstract description 5
- 239000002904 solvent Substances 0.000 abstract description 5
- 239000012535 impurity Substances 0.000 abstract description 2
- 210000004027 cell Anatomy 0.000 abstract 6
- XKRFYHLGVUSROY-UHFFFAOYSA-N Argon Chemical compound [Ar] XKRFYHLGVUSROY-UHFFFAOYSA-N 0.000 description 8
- 238000010438 heat treatment Methods 0.000 description 6
- 229910052786 argon Inorganic materials 0.000 description 4
- 238000000926 separation method Methods 0.000 description 4
- 238000004458 analytical method Methods 0.000 description 3
- 238000010586 diagram Methods 0.000 description 3
- 230000033001 locomotion Effects 0.000 description 3
- 238000012083 mass cytometry Methods 0.000 description 3
- 230000008901 benefit Effects 0.000 description 2
- 239000000112 cooling gas Substances 0.000 description 2
- 238000000605 extraction Methods 0.000 description 2
- 238000004949 mass spectrometry Methods 0.000 description 2
- 230000004931 aggregating effect Effects 0.000 description 1
- 230000006907 apoptotic process Effects 0.000 description 1
- 230000009087 cell motility Effects 0.000 description 1
- 230000004656 cell transport Effects 0.000 description 1
- 238000007796 conventional method Methods 0.000 description 1
- 230000008878 coupling Effects 0.000 description 1
- 238000010168 coupling process Methods 0.000 description 1
- 238000005859 coupling reaction Methods 0.000 description 1
- 238000004807 desolvation Methods 0.000 description 1
- 238000009792 diffusion process Methods 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 238000002309 gasification Methods 0.000 description 1
- 230000006872 improvement Effects 0.000 description 1
- 238000001095 inductively coupled plasma mass spectrometry Methods 0.000 description 1
- 150000002500 ions Chemical class 0.000 description 1
- 230000007246 mechanism Effects 0.000 description 1
- 230000010534 mechanism of action Effects 0.000 description 1
- 238000002663 nebulization Methods 0.000 description 1
- 230000002572 peristaltic effect Effects 0.000 description 1
- 230000008569 process Effects 0.000 description 1
- 230000002035 prolonged effect Effects 0.000 description 1
- 238000011084 recovery Methods 0.000 description 1
- 230000009467 reduction Effects 0.000 description 1
- 238000005070 sampling Methods 0.000 description 1
- 238000004088 simulation Methods 0.000 description 1
- 239000000243 solution Substances 0.000 description 1
- 239000002699 waste material Substances 0.000 description 1
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
- G01N27/62—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating the ionisation of gases, e.g. aerosols; by investigating electric discharges, e.g. emission of cathode
- G01N27/626—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating the ionisation of gases, e.g. aerosols; by investigating electric discharges, e.g. emission of cathode using heat to ionise a gas
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- H—ELECTRICITY
- H01—ELECTRIC ELEMENTS
- H01J—ELECTRIC DISCHARGE TUBES OR DISCHARGE LAMPS
- H01J49/00—Particle spectrometers or separator tubes
- H01J49/02—Details
- H01J49/04—Arrangements for introducing or extracting samples to be analysed, e.g. vacuum locks; Arrangements for external adjustment of electron- or ion-optical components
- H01J49/0404—Capillaries used for transferring samples or ions
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- H—ELECTRICITY
- H01—ELECTRIC ELEMENTS
- H01J—ELECTRIC DISCHARGE TUBES OR DISCHARGE LAMPS
- H01J49/00—Particle spectrometers or separator tubes
- H01J49/02—Details
- H01J49/04—Arrangements for introducing or extracting samples to be analysed, e.g. vacuum locks; Arrangements for external adjustment of electron- or ion-optical components
- H01J49/0431—Arrangements for introducing or extracting samples to be analysed, e.g. vacuum locks; Arrangements for external adjustment of electron- or ion-optical components for liquid samples
- H01J49/0445—Arrangements for introducing or extracting samples to be analysed, e.g. vacuum locks; Arrangements for external adjustment of electron- or ion-optical components for liquid samples with means for introducing as a spray, a jet or an aerosol
- H01J49/045—Arrangements for introducing or extracting samples to be analysed, e.g. vacuum locks; Arrangements for external adjustment of electron- or ion-optical components for liquid samples with means for introducing as a spray, a jet or an aerosol with means for using a nebulising gas, i.e. pneumatically assisted
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- Chemical & Material Sciences (AREA)
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- Health & Medical Sciences (AREA)
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- Dispersion Chemistry (AREA)
- Life Sciences & Earth Sciences (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- General Physics & Mathematics (AREA)
- Immunology (AREA)
- Pathology (AREA)
- Other Investigation Or Analysis Of Materials By Electrical Means (AREA)
Abstract
The invention discloses a high-efficiency sample injection system for single-cell detection, which comprises an atomizer for atomizing a sample to be detected output by an injector to form aerosol, an atomizing chamber for reducing solvent on the cell surface of the sample with super-large particle size, and a concentrator with an inner independent chamber and an outer independent chamber. According to the invention, based on an aerodynamic principle, the cell sample is concentrated, other cells or impurities outside cells of the detection sample are removed, the cell number concentration and ionization efficiency entering a rectangular tube are improved, the number of ionization areas of the cell sample in the center of the rectangular tube is increased, and the detection sensitivity of the instrument is further improved; at the same sensitivity, ICP source RF coil load power requirements are reduced, reducing gas consumption for cooling.
Description
Technical Field
The invention belongs to the technical field of analysis and detection instruments, and particularly relates to a high-efficiency sample injection system for single cell detection and a use method thereof.
Background
Cells are basic units of organism structures and functions, and in order to search for the working mechanism of living organisms, intensive studies on the growth, propagation, apoptosis, inheritance, evolution, and the like of cells are generally required by taking the cells as research targets. However, due to the limitation of conditions, the conventional method usually takes clustered cells as a research object, and obtains information of cells on average. However, there are often large differences between cells, so it is necessary to establish a single cell level-based analytical method to more accurately explain the mechanism of action of cells on vital activities. Currently, single-cell analysis methods based on Mass spectrometry mainly include single-cell ICP-MS (SC-ICP-MS) and Mass Cytometry (Mass Cytometry), wherein the SC-ICP-MS can directly analyze the element types and contents in single cells, and MassCytometry can perform multi-parameter detection on single cells. Both technologies are based on an ICP plasma source, a single-cell sample to be detected is generated through a sample injection system at the front end, then plasma ionization is carried out, and finally detection is carried out through mass spectrometry. There are two problems: 1) The sensitivity of ICP detection on cell samples needs to be further improved, and is often solved by improving ICP radio frequency power, but the consumption of cooling gas is greatly increased; 2) Sample cells enter the torch tube from the atomizing chamber through carrier gas, and the whole beam diameter is divergent, so that the ionization efficiency of ICP is affected.
Aiming at the technical problems, the main solution is to improve the detection sensitivity by improving the structure of a torch tube, an atomizer or a fog chamber. For example, FIG. 1, alexander et al, through a newly designed atomizing system, the cell transport efficiency was 75.0.+ -. 4.7%. But the number concentration of sample cells is still low with more carrier gas and atomizing gas entering the ICP torch tube. The patent (US 2017/0098532) designs a novel atomizing chamber for cell sample injection, the structure of the atomizing chamber is shown in figure 2, the atomizing chamber is divided into a deceleration area and an acceleration area, and the atomizing chamber has a heating function and can remove solvent from sample cells. The method belongs to a traditional method in cell sample injection, and has limited improvement on detection sensitivity. As also shown in fig. 3, alavi et al (WO 2019/144220 A1) design a tapered torch tube that reduces the argon consumption of the cooling gas by 70%, thus increasing the rf power applied to the coil, i.e., increasing the detection sensitivity, under the same conditions. In addition, a learner designs a recovery system of cooling argon gas to indirectly improve the radio frequency power, but the design is complex and difficult to popularize.
Disclosure of Invention
Aiming at the problems existing in the prior art, the invention aims to provide a high-efficiency sample injection system for single cell detection and a using method.
The invention is realized by the following technical scheme:
The high-efficiency sample injection system for single-cell detection is characterized by comprising an atomizer, an atomization chamber and a concentrator;
the atomizer is connected to the injector through an atomization capillary tube and atomizes a sample to be tested output by the injector to form aerosol;
The device comprises an atomization chamber, wherein an atomizer interface is arranged at the front end of the atomization chamber, a tail end outlet is arranged at the rear end of the atomization chamber, a carrier gas branch pipe is arranged on the side wall close to the atomizer interface, the atomizer interface is used for axially inserting and fixing the atomizer along the atomization chamber so as to introduce aerosol into the atomization chamber, the carrier gas branch pipe is used for introducing carrier gas into the atomization chamber, and the carrier gas carries the aerosol and is output from the tail end outlet;
The concentrator, its front end is provided with the atomising chamber connector for the connection installation of atomising chamber, the concentrator has two inside and outside independent cavities, and coaxial arrangement has accelerating hole and receiving hole in the inner chamber, the other end and the quarter butt of receiving hole link to each other, and outer cavity is close to on the lateral wall of exit end and passes through the branch pipe and link to each other with peripheral flowmeter and pump.
Further, the atomizer further comprises an outer atomizer pipe and an inner atomizer pipe which are coaxially arranged with the atomizing capillary, and an atomizing gas branch pipe is arranged on the outer atomizer pipe close to the cell suspension sample injection end. The atomizer is mainly used for generating single-cell samples, injecting cell suspension through a peripheral sample injection pipeline, injecting the cell suspension through an atomization capillary, and atomizing by adopting atomizing gas.
After the cell suspension is acted by an atomizer, cell samples with different particle sizes are obtained, and meanwhile, atomized gas enters an atomization chamber together; in the atomizing chamber, there is generally a heating device for reducing the solvent on the surface of some sample cells with ultra-large particle size; the carrier gas branch pipe in the atomizing chamber is used for introducing carrier gas with a certain flow rate to enable the generated cell sample to enter the next stage concentrator.
In the concentrator, the cell sample is separated from the gas consisting of the carrier gas and the atomized gas in the accelerating hole. The particle size of the sample cells is relatively large, generally in the order of micrometers, so that the movement inertia of the sample cells is far greater than that of gas under the action of an accelerating hole. Therefore, the flow rate of the gas entering the receiving hole of the next stage is reduced by pumping a certain flow rate of the gas through the peripheral flowmeter and the pump, so that the unit volume concentration of the sample cells is improved.
Further, an extension pipe is arranged on the accelerating hole and extends to a connecting port of the atomizing chamber, and the extension pipe is used for being connected with a tail end outlet of the atomizing chamber.
Further, the aperture of the accelerating hole is smaller than that of the receiving hole, and a gap is reserved between the accelerating hole and the receiving hole. The aperture of the accelerating hole is optimized to be 0.5mm-1.5mm, and the gap between the accelerating hole and the receiving hole is optimized to be 1-2 mm.
Further, inner cavity air pumping holes are uniformly distributed on the peripheral wall of the inner cavity, and the inner cavity air pumping holes are arranged corresponding to gaps between the accelerating holes and the receiving holes. The outer chamber is pumped through round holes uniformly distributed on the wall of the inner chamber, and the peripheral flowmeter is connected with the pump to pump the side pipe of the outer chamber.
Further, the accelerating hole is arranged in a conical shape, so that the inertia and gas diffusion of the sample cells can be improved, the receiving hole is arranged in an inverse conical shape, the speed of the received sample cells can be reduced, the contact time of the sample cells and the plasma source can be prolonged, and the ionization efficiency can be improved.
The application method of the high-efficiency sample injection system for single cell detection is characterized by comprising the following steps of:
1) Pumping the prepared cell suspension to be tested from an injector to an atomization capillary in a pumping mode, and injecting the cell suspension to be tested into an atomizer;
2) Synchronously opening an atomizing gas branch pipe, introducing atomizing gas, atomizing a cell suspension by an atomizer under the action of the atomizing gas to obtain a cell sample, and entering an atomizing chamber;
3) And synchronously opening a carrier gas branch pipe to introduce carrier gas, enabling sample cells to enter the concentrator under the combined action of the atomized gas and the carrier gas, separating gas particles, enabling the separated cell samples to quickly pass through the accelerating holes and then enter the receiving holes, enabling the separated cell samples to enter the rectangular pipe under the action of residual gas for subsequent mass spectrum detection, and completing sample injection.
Further, the atomizing gas branch pipe and the carrier gas branch pipe are both provided with flow meters, the flow rate of the atomizing gas is 0.15-0.35L/min, and the total flow rate of the atomizing gas and the carrier gas is 0.8-1.2L/min.
By adopting the technical scheme, the invention has the following advantages:
1) By concentrating the cell sample, the cell number concentration entering the rectangular tube is improved, the number of ionization areas of the cell sample in the center of the rectangular tube is increased, and the detection sensitivity of the instrument is further improved;
2) Through the concentrator, other cells or impurities outside cells of the detection sample are removed through aerodynamic separation, so that the ionization efficiency of the sample to be detected is improved, and the detection sensitivity of the system is improved;
3) At the same sensitivity, ICP source RF coil load power requirements are reduced, reducing gas consumption for cooling.
Drawings
FIG. 1 is a diagram of a prior art atomizing system;
FIG. 2 is a diagram of the prior art cell sampling nebulization chamber;
FIG. 3 is a schematic view of a prior art tapered torch tube;
FIG. 4 is a schematic diagram of a sample injection system according to the present invention;
FIG. 5 is a cross-sectional view of the concentrator of the present invention;
FIG. 6 is a graph of trajectories of cells of different particle sizes based on CFD software simulation in accordance with the present invention;
FIG. 7 is a flow chart of the overall invention;
In the figure, 1-atomizer, 101-atomizing capillary, 102-atomizer outer tube, 103-atomizer inner tube, 104-atomizing gas branch tube, 2-atomizing chamber, 201-atomizer interface, 202-tail end outlet, 203-carrier gas branch tube, 3-concentrator, 301-atomizing chamber connection port, 302-inner chamber, 303-accelerating hole, 304-receiving hole, 305-outer chamber, 306-branch tube, 307-extension tube, 308-gap, 309-inner chamber pumping hole, 4-rectangular tube, 5-peripheral flowmeter, 6-pump.
Detailed Description
The invention is further described below with reference to the drawings in the specification, it being understood that the following description is only illustrative of the invention and is not intended to limit the invention.
As shown in FIG. 4, the high-efficiency sample injection system for single cell detection comprises an atomizer 1, an atomization chamber 2 and a concentrator 3.
Wherein, the atomizer 1 is connected to the injector through an atomization capillary 101, and atomizes a sample to be tested output by the injector to form aerosol; the cell suspension quantitative atomization device comprises an atomizer outer tube 102, an atomizer inner tube 103 and an atomization capillary 101 which are coaxially arranged, wherein the atomizer outer tube 102 is close to a cell suspension sample injection end and is provided with an atomization gas branch tube 104, the atomization gas branch tube 104 is used for introducing atomization gas to atomize cell suspension to be tested, and a flowmeter is arranged on the atomizer outer tube and is used for quantitatively introducing the atomization gas.
The front end of the atomizing chamber 2 is provided with an atomizer interface 201, the rear end of the atomizing chamber is provided with a tail end outlet 202, a carrier gas branch pipe 203 is arranged on the side wall close to the atomizer interface 201 and used for introducing a certain amount of carrier gas, so that a generated cell sample enters the next-stage concentrator 3, and the atomizer interface 201 is used for axially inserting and fixing the atomizer 1 along the atomizing chamber 2, so that aerosol is introduced into the atomizing chamber 2.
The concentrator 3, its front end is provided with the atomizer chamber connector 301 for the connection installation of atomizer chamber 2, and this concentrator 3 has two independent cavities in, outer, installs the accelerating hole 303 of toper structure and the receiving hole 304 of anti-toper structure coaxially in the inner chamber 302, is provided with extension pipe 307 on the accelerating hole 303 and extends to atomizer chamber connector 301 for link to each other with the tail end export of atomizer chamber 2, and the other end of receiving hole 304 links to each other with quarter butt 4, and outer cavity 305 is close to on the lateral wall of exit end and is linked to each other with peripheral flowmeter 5 and pump 6 through branch pipe 306. The aperture of the accelerating hole 303 is smaller than that of the receiving hole 304, a gap 308 is reserved between the accelerating hole 303 and the receiving hole 304, and inner cavity air pumping holes 309 are uniformly distributed on the circumferential wall of the inner cavity 302 at annular positions corresponding to the gap 308.
As shown in the half-section of the concentrator 3, the accelerating aperture 303 is seen on the left of the structure, connected to the atomising chamber 2 by an extension tube 307. The accelerating hole 303 has a diameter of 0.8mm, the receiving hole 304 has a diameter of 1mm, and the end surfaces of the two holes are spaced apart by 1mm. In the structure of the concentrator 3, six inner pumping holes 309 are uniformly distributed on the wall of the inner chamber 302, and a branch pipe 306 is used for connecting the peripheral flowmeter 5 and the pump 6 at the outlet side of the outer chamber 305. The outlet of the concentrator 3 is connected with a rectangular tube 4.
The application method of the sample injection system comprises the following specific steps:
1) Introducing the prepared cell suspension to be tested into the atomizer 1 through a peristaltic pump, a syringe pump or other modes;
2) The atomized gas is introduced through the atomized gas branch pipe 104 by a flowmeter, and for a cell sample, the atomized gas flow is generally low, and for the example of a current commercial instrument, the atomized gas flow is generally 0.15-0.35L/min;
3) Under the action of atomizing gas, the cell suspension is atomized by the atomizer 1 and enters the atomizing chamber 2, the particle size of the cells of the sample is generally less than 10 mu m, the central particle size is a few mu m, but the cells with the particle size larger than 10 mu m and the cells with the smaller particle size are also arranged at the same time;
4) The carrier gas is introduced into the atomizing chamber 2 through the carrier gas branch pipe 203 by the flowmeter to bring the cell sample into the next stage system, and the general flow is about 1L/min. The atomizing chamber 2 can reduce the solvent on the surface of the cell sample by heating, reduce the particle size of the cells, and prevent the cell sample from aggregating into large clusters on the surface of the atomizing chamber 2; the heating device arranged on the atomizing chamber 2 is in the prior art, and the heating device is not specifically described and can be used with reference to the prior art;
5) The cell sample enters the concentrator 3 under the action of the atomizing gas and the carrier gas, and because the inertia of the gas (or small-particle-size cell sample) is much smaller than that of the cell sample (μm magnitude), the gas expands and diverges at the acceleration hole, and the cell sample can pass through the acceleration hole 303 and then enter the receiving hole 304. In the process of gas particle separation, an external pump and a flowmeter are adopted to extract gas, and finally, the gas is discharged through a peripheral waste discharge system. For example, the total flow rate of the atomizing gas and the carrier gas is 1L/min, and the flow rate of the residual gas entering the torch tube along with the cell sample is 0.1L/min by pumping 0.9L/min, namely, the cell number concentration is improved by 10 times, the concentration is expressed on a mass spectrum signal, and the signal to be detected is theoretically improved by 10 times;
6) The cell sample is subjected to gas particle separation, so that the cell concentration of the sample is concentrated, and the sample enters the rectangular tube 4 under the action of the residual gas, and as the cell number concentration of the sample is improved, the mass spectrum signal is synchronously improved, and the sensitivity of the whole instrument is improved in an order of magnitude. Meanwhile, based on the method, the method has the additional advantage that the amount of the atomized gas and the carrier gas entering the ICP source is greatly reduced, the influence of the gas on the distribution of plasma center torches can be effectively reduced, and the consumption of the small-particle-size cell sample on ICP can be reduced along with the pumping due to weak inertia. On the other hand, considering the problem of cooling argon consumption, we can reduce the cooling argon while keeping the instrument detection sensitivity at a relatively good level, with appropriate reduction of the RF coil load power.
As shown in fig. 6, a sample cell motion trajectory simulation was performed on the structure by CFD software based on the established concentrator simple model. Three particle size cells with particle size Dp=0.1 mu m, 0.5 mu m, 2 mu m and the like are selected as study objects, the total air inflow flow is 1L/min, the cells enter a concentrator together with the sample cells, the pumping flow is 0.1L/min, and the motion trail of the sample cells is obtained through coupling of the CFD module and the particle tracking module. It was found that when dp=0.1 μm, the sample cells had only 8% of their passage through the receiving well, most of which was eliminated with pumping; when dp=0.5 μm, the sample cells had a 42% ratio of inertial entry into the receiving well; when dp=2 μm, the sample cells were able to achieve 100% extraction, and the overall beam diameter was constrained. Therefore, based on the method, efficient extraction of sample cells can be achieved.
The application flow of the invention is shown in figure 7, the cell suspension is introduced by an injection pump or an automatic sampler, and is input into an atomizer by a hose, an atomization capillary tube in the middle of the atomizer outputs a sample, and the sample is atomized under the action of branch atomization gas. The single cell sample enters an atomizing chamber, and a heating device is wrapped outside the atomizing chamber and used for removing the solvent of the sample cells with the ultra-large particle size. Under the action of carrier gas and atomized gas, the sample cells enter the accelerating holes. At this time, since the flowmeter and the pump are arranged at the periphery, the separation of the sample cells and the gas is realized by extracting a certain gas flow, namely, the sample cells are concentrated, then pass through the receiving hole, the movement speed of the sample cells is reduced in the receiving hole, then enter the torch tube together with the rest gas, and the desolvation, gasification and ionization are realized at the plasma source at the tail end of the torch tube. Finally, the ions pass through a vacuum interface and enter mass spectrum detection.
Claims (8)
1. The efficient sample injection system for single-cell detection is characterized by comprising an atomizer (1), an atomization chamber (2) and a concentrator (3);
the atomizer (1) is connected to the injector through an atomization capillary (101) and atomizes a sample to be tested output by the injector to form aerosol;
the device comprises an atomization chamber (2), wherein an atomizer interface (201) is arranged at the front end of the atomization chamber, a tail end outlet (202) is arranged at the rear end of the atomization chamber, a carrier gas branch pipe (203) is arranged on the side wall close to the atomizer interface (201), the atomizer interface (201) is used for axially inserting and fixing the atomizer (1) along the atomization chamber (2) so as to introduce aerosol into the atomization chamber (2), the carrier gas branch pipe (203) is used for introducing carrier gas into the atomization chamber (2), and the carrier gas carries the aerosol to be output from the tail end outlet (202);
Concentrator (3), its front end is provided with atomising chamber connector (301) for the connection installation of atomising chamber (2), concentrator (3) have inside and outside two independent cavities, and coaxial installation has accelerating hole (303) and receiving hole (304) in inner chamber (302), the other end and the quarter wave tube (4) of receiving hole (304) link to each other, and outer chamber (305) are close to on the lateral wall of exit end and link to each other with peripheral flowmeter (5) and pump (6) through branch pipe (306).
2. The efficient sample injection system for single-cell detection as claimed in claim 1, wherein the atomizer (1) further comprises an atomizer outer tube (102) and an atomizer inner tube (103) which are coaxially arranged with the atomizing capillary tube (101), and an atomizing gas branch tube (104) is arranged on the atomizer outer tube (102) close to the sample injection end of the cell suspension.
3. The high-efficiency sample injection system for single-cell detection as claimed in claim 1, wherein the acceleration hole (303) is provided with an extension tube (307) extending to a connection port of the atomizing chamber (2) for connection with a tail end outlet of the atomizing chamber (2).
4. The high-efficiency sample injection system for single-cell detection as claimed in claim 1, wherein the aperture of the acceleration hole (303) is smaller than the aperture of the receiving hole (304), and a gap (308) is left between the acceleration hole (303) and the receiving hole (304).
5. The efficient sample injection system for single-cell detection as claimed in claim 1, wherein inner cavity gas pumping holes (309) are uniformly distributed on the peripheral wall of the inner cavity (302), and the inner cavity gas pumping holes (309) are arranged corresponding to gaps (308) between the accelerating holes (303) and the receiving holes (304).
6. The high-efficiency sample injection system for single-cell detection as claimed in claim 1, wherein the acceleration hole (303) is arranged in a tapered shape, and the receiving hole (304) is arranged in an inverse tapered shape.
7. The application method of the high-efficiency sample injection system for single cell detection is characterized by comprising the following steps of:
1) Pumping the prepared cell suspension to be tested from a syringe to an atomization capillary (101) in a pumping mode, and injecting the cell suspension to be tested into an atomizer (1);
2) Synchronously opening an atomizing gas branch pipe (104), introducing atomizing gas, atomizing a cell suspension by an atomizer (1) under the action of the atomizing gas to obtain a cell sample, and entering an atomizing chamber (2);
3) And synchronously opening a carrier gas branch pipe (203) to introduce carrier gas, enabling sample cells to enter a concentrator (3) under the combined action of the atomized gas and the carrier gas, separating gas particles, enabling separated cell samples to quickly pass through an acceleration hole (303) and then enter a receiving hole (304), and enabling the separated cell samples to enter a rectangular pipe (4) under the action of residual gas to carry out subsequent mass spectrum detection, so that sample injection is completed.
8. The method of claim 7, wherein the atomizing gas branch pipe (104) and the carrier gas branch pipe (203) are respectively provided with a flowmeter, the flow rate of the atomized gas is 0.15-0.35L/min, and the total flow rate of the atomized gas plus the carrier gas is 0.8-1.2L/min.
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| CN215988660U (en) * | 2021-09-26 | 2022-03-08 | 谱视科技(杭州)有限公司 | A high-efficient sampling system for unicellular detection |
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| CA2673912A1 (en) * | 2006-12-29 | 2008-07-10 | Scott D. Tanner | Cell injector for flow cytometer having mass spectrometer detector and method for using same |
| WO2013061155A1 (en) * | 2011-10-26 | 2013-05-02 | Dvs Sciences Inc. | Sample transferring apparatus for mass cytometry |
| CN110426445B (en) * | 2019-08-01 | 2021-07-27 | 东北大学 | Three-dimensional ordered helical focusing nanoparticle/single cell analysis system and method of use |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN215988660U (en) * | 2021-09-26 | 2022-03-08 | 谱视科技(杭州)有限公司 | A high-efficient sampling system for unicellular detection |
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