CN113769805A - Micro-fluidic device for realizing material mixing in micro-fluidic chip and mixing control method - Google Patents
Micro-fluidic device for realizing material mixing in micro-fluidic chip and mixing control method Download PDFInfo
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Abstract
The invention provides a microfluidic device for realizing material mixing in a microfluidic chip and a mixing control method, wherein the microfluidic device comprises a microfluidic chip body, a first containing bottle body and a second containing bottle body, wherein the first containing bottle body and the second containing bottle body are respectively used for containing different materials, a microfluidic pipeline is constructed in the microfluidic chip body, a needle body with a needle opening is respectively and correspondingly arranged on the first containing bottle body and the second containing bottle body, the two containing bottle bodies can be communicated with the microfluidic pipeline through the needle bodies respectively, the first containing bottle body and the second containing bottle body can linearly move along the axial direction of a bottle sleeve under the action of axial force so that the needle bodies are converted into a circulation state from a first sealing state, and a second sealing component can be close to the first sealing component under the action of the axial force so that the materials are released. According to the invention, the materials in the two accommodating bottles can flow between each other to realize uniform mixing of the materials, so that the accuracy of the detection result is ensured.
Description
Technical Field
The invention belongs to the technical field of consumables for biological experiments, and particularly relates to a micro-fluidic device for realizing material mixing in a micro-fluidic chip and a mixing control method.
Background
Molecular diagnosis is a comprehensive and comprehensive clinical diagnosis technology which detects contents, types and coding information of biomolecules including nucleic acids, proteins, saccharides and other substances in a human body and combines related contents of biomolecular science, and is mainly applied to the fields of diagnosis of genetic diseases, control of infectious diseases and tumor treatment at present. However, the sample collected from the patient is generally complicated and contains many substances that inhibit or interfere with the detection, and therefore, many pretreatment processes are required to purify the substance to be detected and perform qualitative or quantitative detection. The conventional molecular diagnosis process generally transfers clinical samples to a detection laboratory with related qualification, depends on related equipment of the laboratory and professional operators to perform pretreatment of the samples, and is uniformly operated to perform biochemical detection or analysis. In the process, a long time is delayed, and the detection of some sudden diseases, large-scale infectious diseases and complex diseases is difficult to carry out in real time and in place. In addition, such a testing laboratory requires expensive testing equipment and specialized laboratory staff, and is difficult to be popularized in resource-poor areas.
The advent of microfluidic technology has addressed some of the problems in molecular diagnostics, and microfluidic chips have miniaturized the modules of biochemical assays and integrated them together via microchannels. The chip realizes the operations of accurate distribution of fluid, heating of reactants, uniform mixing of reagents, fluorescence detection and the like under the control of a matched instrument, thereby completing the complex, time-consuming and labor-consuming detection in the traditional laboratory at low cost in a short time and realizing the real 'sample input-result output'. However, in order to realize high-efficiency amplification on a chip, one of the important difficulties is to mix reagents uniformly, and generally, the whole chip is totally enclosed, and the channels of the chip are narrow, so that the reagents to be mixed uniformly are less, and the uniformity of the reagents is difficult to ensure, and thus the detection accuracy cannot be ensured. Therefore, only by controlling the movement of the fluid, the sufficient and efficient mixing of the materials among the steps can be ensured, and the problems of high cost, poor sensitivity, complex structure, excessively complex operation and related detection equipment and the like in the prior art can be avoided.
Disclosure of Invention
Therefore, the technical problem to be solved by the present invention is to provide a microfluidic device for realizing material mixing in a microfluidic chip and a mixing control method, wherein the accommodating bottle bodies and the microfluidic chip are integrated into a whole, and the materials in the two accommodating bottle bodies can flow between each other to realize uniform mixing of the materials, so as to ensure the accuracy of the detection result.
In order to solve the above problems, the present invention provides a microfluidic device for uniformly mixing materials in a microfluidic chip, comprising a microfluidic chip body, and a first containing bottle body and a second containing bottle body for containing different materials, wherein the microfluidic chip body is internally configured with a microfluidic channel, the first containing bottle body and the second containing bottle body are respectively located in a bottle sleeve arranged on the microfluidic chip body, the first containing bottle body and the second containing bottle body are respectively and correspondingly provided with a needle body having a needle opening, the two containing bottle bodies can be communicated with the microfluidic channel through the needle body respectively, a first sealing assembly and a second sealing assembly are respectively connected to a first end and a second end of the first containing bottle body in an axial direction to form an internal sealed containing space of the first containing bottle body, the second containing bottle body is a bottom sealed bottle body, and an opening part of the second containing bottle body is fixedly connected with the first sealing assembly, the needle body has the needle mouth is in first sealed state in the first seal assembly, the needle mouth is in the circulation state in the inside seal accommodation space, first holding bottle, second holding bottle can follow the axial linear motion of bottle cover under the effect of axial force so that the needle body is converted into the circulation state by first sealed state, the second seal assembly can be close to first seal assembly under the effect of axial force so that the material releases.
Preferably, the needle body further has a second sealing state of the needle opening in the second sealing assembly, and the second sealing assembly is further capable of switching the needle body from the flow-through state to the second sealing state under the action of the axial force.
Preferably, the first sealing assembly comprises a first rubber plug and a protective cover, and when the needle body is in the first sealing state, the needle opening is in the first rubber plug; the second sealing component comprises a second rubber plug and a hard gasket positioned at one side of the second rubber plug, which deviates from the first rubber plug, and the needle body is positioned in the second rubber plug when in the second sealing state.
Preferably, the first end is of a throat structure, the first rubber plug is a first convex rubber plug, and a convex protrusion of the first convex rubber plug is embedded in the throat structure; and/or a sealing convex ring is arranged on the outer circumferential wall of the second rubber plug; and/or a first stop ring which is convex towards the radial inner side of the accommodating bottle body is arranged on the inner circumferential wall of the second end of the accommodating bottle body, and the inner circle diameter of a ring body of the first stop ring is smaller than that of the second rubber plug.
Preferably, the second rubber plug is a second convex-shaped rubber plug, and the shape of the convex-shaped protruding part of the second convex-shaped rubber plug can be matched with the shape of the necking structure, so that the materials in the inner sealed containing space are completely released and discharged through the needle opening.
Preferably, one end of the bottle sleeve, which is far away from the microfluidic chip body, is provided with a second stop ring which is convex inwards along the radial direction of the bottle sleeve; and/or the needle opening is positioned on the circumferential side wall of the needle body.
Preferably, the number of the first containing bottle bodies is two, the microfluidic pipeline has two sections which are not communicated, one section of the microfluidic pipeline can communicate one of the two first containing bottle bodies with the second containing bottle body, and the other section of the microfluidic pipeline can communicate the other of the two first containing bottle bodies with the second containing bottle body.
The invention also provides a mixing control method of the microfluidic device, which is used for controlling the microfluidic device for realizing material mixing in the microfluidic chip, and comprises the following steps:
controlling to apply axial force to a second sealing assembly of the first accommodating bottle body and the bottle bottom of the second accommodating bottle body so as to enable the first accommodating bottle body and the second accommodating bottle body to move close to the needle body and enable the needle body to be converted from the first sealing state to the circulation state respectively;
and controlling to release the axial force applied to the bottom of the second accommodating bottle body and continuously apply the axial force to the second sealing assembly of the first accommodating bottle body, releasing the axial force applied to the second sealing assembly before the needle body corresponding to the needle body is converted from the circulation state to the second sealing state, applying the axial force to the second sealing assembly again after preset time, and controlling to release the axial force applied to the second sealing assembly of the first accommodating bottle body after the axial force is applied at intervals for preset times.
The invention also provides a mixing control method of the microfluidic device, which is used for controlling the microfluidic device for realizing material mixing in the microfluidic chip, and comprises the following steps:
controlling to apply axial force to the two first containing bottle bodies and the second containing bottle bodies to enable the corresponding containing bottle bodies to move close to the needle bodies and enable the needle bodies to be converted from the first sealing state to the circulation state respectively;
controlling to release the axial force applied to the second sealing component of the second bottle-containing body and continue to apply the axial force to the second sealing component of the first bottle-containing body to force the material therein to flow into the second bottle-containing body through the second bottle-containing body until the axial force applied to the corresponding needle body is released before the needle body is converted from the flow-through state to the second sealing state;
and controlling the axial force applied to the second sealing component of the second accommodating bottle body to enable the second sealing component of the second accommodating bottle body to move close to the needle body so as to force the material in the second accommodating bottle body to flow into the first accommodating bottle body through the second accommodating bottle body until the axial force applied to the corresponding needle body is released before the needle body is converted from the flow state to the second sealing state, and applying force to the second sealing component of the first accommodating bottle body again, so that after the axial force is applied alternately for a preset number of times, the axial force applied to the first accommodating bottle body and the second accommodating bottle body is released after the corresponding needle body in the first accommodating bottle body and the second accommodating bottle body is controlled to be converted from the flow state to the second sealing state.
The invention provides a microfluidic device for realizing material mixing in a microfluidic chip and a mixing control method, wherein a containing bottle body and a second sealing component can move close to a needle body under the action of axial force, and the needle body is switched from a first sealing state to a circulating state in the moving process, so that the material is released into a microfluidic pipeline from the first containing bottle body and enters another second containing bottle body with different materials through the microfluidic pipeline, thereby realizing the integrated design between the microfluidic chip and the containing bottle body and the automatic mixing of the different materials at the same time, realizing the reciprocating release and uniform mixing of the materials on the compression force in the second sealing component of the first containing bottle body and the second containing bottle body which exert the axial force at intervals, and further ensuring the accuracy of a detection result, the automation degree of the microfluidic device is improved.
Drawings
Fig. 1 is a schematic partial structure diagram of a microfluidic device for achieving material mixing in a microfluidic chip according to an embodiment of the present invention, in which a needle is shown in a first sealing state;
FIG. 2 is an enlarged partial schematic view of FIG. 1;
FIG. 3 is a schematic view of a structure of the needle body of FIG. 1;
FIG. 4 is another schematic view of the needle body of FIG. 1;
FIG. 5 is a further schematic view of the needle body of FIG. 1;
fig. 6 shows a state change of a containing bottle body in a microfluidic device for realizing material mixing in the microfluidic chip according to an embodiment of the present invention after an axial force is applied to the containing bottle body, where (a) the needle body is in a first sealing state, (b) and (c) the needle body is in a flow state, and (d) the needle body is in a second sealing state, and this process realizes that a material in the containing bottle body is released and flows out under the axial force and is finally sealed again;
fig. 7 to 10 are schematic diagrams showing states of two containing bottles in a process of mixing different materials in the two containing bottles in a case that two containing bottles are arranged in a microfluidic device for realizing material mixing in a microfluidic chip, wherein a material in a bottle a is a liquid S, and a material in a bottle B is a soluble liquid S;
fig. 11 to 16 show the state of each receiving bottle body in the process of mixing different materials in three receiving bottle bodies, in the case that three receiving bottle bodies are provided in the microfluidic device for realizing material mixing in the microfluidic chip, wherein the material in the bottle C is soluble solid T, the material in the bottle a is liquid S, and the material in the bottle B is not provided.
The reference numerals are represented as:
1. a microfluidic chip body; 11. a microfluidic conduit; 2. a first receiving vial; 21. a first stop ring; 3. a bottle sleeve; 31. a second stop ring; 4. a needle body; 41. a needle opening; 51. a first rubber plug; 52. a protective cover; 61. a second rubber plug; 611. a sealing convex ring; 62. a hard pad; 9. the second receiving bottle body.
Detailed Description
Referring to fig. 1 to 16 in combination, according to an embodiment of the present invention, a microfluidic device for uniformly mixing materials in a microfluidic chip is provided, which includes a microfluidic chip body 1, and a first containing bottle body 2 and a second containing bottle body 9 for containing different materials (one of the materials needs to be in a liquid state), a microfluidic channel 11 is configured in the microfluidic chip body 1, the first containing bottle body 2 and the second containing bottle body 9 are respectively located in a bottle sleeve 3 disposed on the microfluidic chip body 1, the first containing bottle body 2 and the second first containing bottle body 2 are respectively and correspondingly provided with a needle 4 having a needle opening 41, the two containing bottle bodies can be communicated with the microfluidic channel 11 through the needle 4 respectively provided, a first sealing component and a second sealing component are respectively connected to a first end and a second end of the first containing bottle body 2 in an axial direction to form an inner sealed containing space of the first containing bottle body 2, the second containing bottle body 9 is a bottom sealing bottle body, the mouth of the second containing bottle body 9 is fixedly connected with the first sealing assembly, the needle body 4 has a first sealing state that the needle opening 41 is in the first sealing assembly, the needle opening 41 is in a circulation state in the internal sealing containing space, the first containing bottle body 2 and the second containing bottle body 9 can move linearly along the axial direction of the bottle sleeve 3 under the action of axial force to enable the needle body 4 to be converted from the first sealing state into the circulation state, and the second sealing assembly can be close to the first sealing assembly under the action of the axial force to enable the materials to be released, in particular, the materials in the first containing bottle body 2 are released into the second containing bottle body 9 to achieve material mixing. In the technical scheme, the accommodating bottle body and the second sealing component can move close to the needle body under the action of the axial force, and the needle body is switched from a first sealing state to a circulating state in the moving process, so that the material is released from the first receiving vial 2 into the microfluidic channel and passes through the microfluidic channel into another second receiving vial 9 with a different material, thereby realizing the integrated design between the micro-fluidic chip and the accommodating bottle body and simultaneously realizing the automatic mixing of different materials, and the reciprocating release and uniform mixing of the materials can be realized through applying axial force to the second sealing component of the first containing bottle body 2 and the compression force in the second containing bottle body 9 at intervals, so that the accuracy of the detection result is ensured, and the automation degree of the microfluidic device is improved.
It should be noted that the jacket 3 is able to constrain the radial displacement of the first containing body 2 without limiting the axial displacement of the first containing body 2.
In some embodiments, the needle body 4 further has a second sealing state in which the needle port 41 is located in the second sealing assembly, and the second sealing assembly is further capable of finally switching the needle body 4 from the flow-through state to the second sealing state under the action of the axial force, and in particular, after the material in the first accommodating bottle body 2 is completely released, the needle body 4 can be located in the second sealing state, that is, the needle port 41 is sealed again, which can adapt to a situation that the microfluidic device has a plurality of first accommodating bottle bodies 2, and the release of the internal material has a certain sequence, so as to prevent the material in other accommodating bottle bodies from being released into the released accommodating bottle body without entering or only partially entering the first accommodating bottle body 2 that needs to enter.
The specific structural forms of the first sealing component and the second sealing component are various, but it should be noted that the first sealing component may be specifically implemented in a fixed sealing manner due to the absence of the requirement for displacement, specifically, for example, the first sealing component includes a first rubber plug 51 and a protective cover 52, the protective cover 52 may be an aluminum cover (the specific material is selected based on the principle that the needle 4 can be smoothly punctured), and at this time, when the needle 4 is in the first sealing state, the needle opening 41 is located in the first rubber plug 51. The second sealing component comprises a second rubber plug 61 and a hard gasket 62 which is arranged on one side of the second rubber plug 61, wherein the second rubber plug 61 deviates from the first rubber plug 51, the needle body 4 is arranged in the second sealing state, the needle opening 41 is arranged in the second rubber plug 61, the hard gasket 62 has certain rigidity, and the deformation of the hard gasket is small so as to ensure the effective transmission of the axial force.
In some embodiments, the first end is a throat structure, the first rubber plug 51 is a first rubber plug with a convex shape, the convex protrusion of the first rubber plug is embedded in the throat structure, so that the first rubber plug with a convex shape is axially positioned in the first accommodating bottle body 2 through the structure of the first rubber plug with a convex shape, and the second rubber plug 61 is a second rubber plug with a convex shape, and the shape of the convex protrusion of the second rubber plug with a convex shape can be matched with the shape of the throat structure, so that the material in the inner sealed accommodating space is completely released and discharged through the needle opening 41, and the utilization rate of the material is improved.
In order to effectively prevent the leakage of the material, preferably, a sealing convex ring 611 is arranged on the outer circumferential wall of the second rubber plug 61, and a plurality of sealing convex rings 611 can be arranged at intervals along the axial direction of the second rubber plug 61.
In some embodiments, the inner circumferential wall of the second end of the first bottle accommodating body 2 is provided with a first stop ring 21 protruding toward the radial inner side of the first bottle accommodating body 2, and the inner circular diameter of the ring body of the first stop ring 21 is smaller than the outer circular diameter of the second rubber plug 61, so that the second rubber plug 61 can be prevented from falling out of the first bottle accommodating body 2 from the second end, and material leakage can be effectively prevented. One end of the bottle sleeve 3, which is far away from the microfluidic chip body 1, is provided with a second stop ring 31 which protrudes inwards along the radial direction of the bottle sleeve, so that the first accommodating bottle body 2 and all parts assembled with the first accommodating bottle body can be limited in the bottle sleeve 3, and the first accommodating bottle body is prevented from falling out.
One end of the bottle sleeve 3 close to the microfluidic chip body 1 is connected with the microfluidic chip body 1 in a buckling or ultrasonic bonding mode after the first accommodating bottle body 2 is arranged in the bottle sleeve 3.
The needle port 41 can be opened at the top end of the needle body 4, and preferably, the needle port 41 is located on the circumferential side wall of the needle body 4, as shown in fig. 3 to 5, so when the needle body 4 is in the first sealing state and the second sealing state, if the needle body receives the reverse thrust pressure of the material in the microfluidic pipeline 11, the needle body can extrude the side wall of the rubber plug, an upward force cannot be generated, and then the rubber plug is bounced to cause leakage, that is, the rubber plug can be bounced when the material is reversely pushed, thereby ensuring the sealing effect of the needle port 41 and effectively preventing the leakage of the material.
Preferably, there are two first containing bottle bodies 2, the microfluidic pipeline 11 has two sections that are not communicated, one section of the microfluidic pipeline 11 can communicate one of the two first containing bottle bodies 2 with the second containing bottle body 9, the other section of the microfluidic pipeline 11 can communicate the other of the two first containing bottle bodies 2 with the second containing bottle body 9, and there is no material or different material in one of the two first containing bottle bodies 2, so that a greater variety of materials can be mixed.
According to an embodiment of the present invention, there is also provided a mixing control method for a microfluidic device, for controlling the microfluidic device having a first receiving bottle 2 and a second receiving bottle 9 to mix materials uniformly, the mixing control method including:
controlling the axial force to be applied to the second sealing component of the first containing bottle body 2 and the bottom of the second containing bottle body 9 so as to move the first containing bottle body 2 and the second containing bottle body 9 close to the needle body 4, and converting the needle body 4 from the first sealing state to a flow state respectively;
and controlling to release the axial force applied to the bottom of the second containing bottle body 9 and continue to apply the axial force to the second sealing component of the first containing bottle body 2, releasing the axial force applied to the second sealing component before the needle body 4 corresponding to the second containing bottle body is converted from the circulation state to the second sealing state, applying the axial force to the second sealing component again after preset time, and controlling to release the axial force applied to the second sealing component of the first containing bottle body 2 after the axial force is applied for preset times at intervals.
According to an embodiment of the present invention, there is also provided a blending control method for a microfluidic device, for controlling the microfluidic device having two first receiving bottle bodies 2 and one second receiving bottle body 9 to achieve material blending, including:
controlling the axial force applied to the two second sealing components respectively arranged on the first accommodating bottle body 2 and the second accommodating bottle body 9 to enable the corresponding accommodating bottle bodies to move close to the needle body 4 and enable the needle body 4 to be respectively converted from the first sealing state to the circulating state;
controlling to release the axial force applied to the second bottle-containing sealing member and to continue to apply the axial force to the first bottle-containing sealing member to force the material therein to flow into the second bottle-containing body through the second bottle-containing body 9 until the axial force applied to the corresponding needle body 4 is released before the needle body is switched from the flow-through state to the second sealing state, wherein the first bottle-containing body is one of the two first bottle-containing bodies 2 initially containing the material (or one of the first material) and the second bottle-containing body is one of the two first bottle-containing bodies 2 initially containing no material (or one of the second material);
and controlling the second sealing assembly of the second containing bottle body to apply axial force to enable the second sealing assembly of the second containing bottle body to move close to the needle body 4 so as to force the material in the second containing bottle body to flow into the first containing bottle body through the second containing bottle body 9 until the axial force application to the needle body 4 corresponding to the second containing bottle body is released before the needle body 4 is converted from the flow state into the second sealing state, and applying force to the second sealing assembly of the first containing bottle body again, so that after the axial force is applied alternately for a preset number of times, after the needle body 4 corresponding to one of the first containing bottle body and the second containing bottle body is controlled to be converted from the flow state into the second sealing state, the axial force application to the first containing bottle body and the second containing bottle body is released.
It will be understood that the first receiving flask, now relieved of its force, has sufficient space for the second sealing member to move up under the action of the material entering it, whereas in theory the volume of the first receiving flask should be greater than the total volume of material in both receiving flasks, so as to achieve mixing of the material in the first receiving flask.
Example 1:
the process of mixing the materials with one first bottle 2 and one second bottle 9 is further described below with reference to fig. 7 to 10:
before the device is not used, when a bottle A (in which a liquid material S is placed) and a bottle B (in which a solid material T is placed) are placed inside a bottle sleeve (namely the bottle sleeve 3, the needle body 4 and the needle body are the same below), a part of a rubber plug (namely the first rubber plug 51 and the needle body) of a bottle opening is just pricked by a needle (namely the needle opening 41 and the needle body are the same below), so that the tail end of the needle (namely the needle opening 41 and the needle body) is sealed, meanwhile, a fluid pipeline (namely the microfluidic pipeline 11 and the needle body) on the lower portion of the microfluidic bottle sleeve is sealed, the needle cannot prick the rubber plug, the sealing performance of biological materials inside a bottle body is not damaged, and the needle is located inside the rubber plug. As shown in fig. 7.
When the material mixing device is started, the upper parts of the bottle A and the bottle B are sequentially subjected to downward force (namely, the axial force and the downward force are the same), so that the bottle stopper is punctured by the needle, and two outlets for mixing biological materials to be mixed are opened, as shown in fig. 8.
The pressure is continuously applied to the bottom of the bottle A (namely, the second sealing component), because the whole bottle body is limited in the bottle sleeve, and meanwhile, the outlets of the two bottle bodies respectively filled with different biological materials are also punctured by the needles to be opened, at this time, the rubber plug at the bottom of the bottle A can slowly move under the driving of the downward pressure, and simultaneously the materials in the bottle A are discharged, because the rubber plug of the bottle B is also punctured at this time, and the upper part of the bottle B has a larger space, the air in the bottle B is compressed under the driving of the pressure, and the materials in the bottle A enter the bottle B and are uniformly mixed with the bottle A, as shown in fig. 9.
After the pressure at the bottom of the bottle A is released (i.e. the axial force applied to the bottle A is released), the compressed air rebounds because the air at the upper part of the bottle B is compressed, and the material in the bottle B is squeezed back into the bottle A, as shown in fig. 10. The materials in the bottle A and the bottle B can be fully and uniformly mixed by repeating the steps for many times.
Example 2:
the process of mixing the materials with the two first bottles 2 and the second bottle 9 is further described below with reference to fig. 11 to 16:
before the device is not used, when the bottle A, the bottle B and the bottle C are placed inside the bottle sleeve, the needle just pricks a part of a rubber plug of a bottle opening, so that the tail end of the needle is sealed, and meanwhile, a fluid pipeline at the lower part of the bottle sleeve is also sealed. But the needle can not puncture the rubber plug and does not damage the sealing performance of the biological materials in the bottle body, namely the needle is positioned in the rubber plug. As shown in fig. 11.
When the material blending device is started, the upper parts of the bottle A, the bottle B and the bottle C are sequentially stressed downwards, so that the needles pierce the bottle stopper and open three outlets for blending biological materials, as shown in fig. 12.
Continue to exert pressure to A bottle bottom, because whole bottle is the restriction inside the bottle cover, the export of the three bottle that is equipped with different biological material respectively simultaneously also is punctured by the needle and opens, at this moment, the plug at the bottom of A bottle is under the drive of downward pressure, can slow moving, discharge the inside material of A bottle simultaneously, because the plug of B bottle also is punctured this moment, the plug of B bottle is located the nearer position department of bottleneck, have the space of activity upwards, consequently, the material in the A bottle is under the drive of pressure, through C bottle, and the material mixing in the C bottle, and further get into B bottle and carry out the mixing with its material, the bottom plug of B bottle also can the rebound simultaneously, its process is as shown in figure 13.
The liquid in the bottle A is fully (or partially) pumped into the bottle B through the bottle C, the bottom rubber plug of the bottle A descends to a position close to the bottle mouth at this time, and the needle is not pricked into the bottom rubber plug at the time, as shown in fig. 14.
The mixed material in the bottle B is driven into the bottle A through the bottle C, and the process is similar to the process of driving the bottle A into the bottle B through the bottle C, as shown in fig. 15 and 16. The above process can be repeated for a plurality of times according to the requirement to realize the sufficient mixing of the materials in the bottles A, B and C. It is noted that at least one of the materials in the bottles A and B is in a liquid state.
This kind of mixing structure can increase the figure of C bottle according to the demand, can realize the mixing to multiple material.
It is readily understood by a person skilled in the art that the advantageous ways described above can be freely combined, superimposed without conflict.
The present invention is not limited to the above preferred embodiments, and any modifications, equivalent substitutions and improvements made within the spirit and principle of the present invention should be included in the protection scope of the present invention. The above is only a preferred embodiment of the present invention, and it should be noted that, for those skilled in the art, several improvements and modifications can be made without departing from the technical principle of the present invention, and these improvements and modifications should also be regarded as the protection scope of the present invention.
Claims (9)
1. The utility model provides a realize micro-fluidic device of material mixing among micro-fluidic chip, its characterized in that, hold bottle (9) including micro-fluidic chip body (1) and first holding bottle (2) and the second that are used for holding different materials respectively, micro-fluidic chip body (1) inner structure has micro-fluidic pipeline (11), first holding bottle (2) and second holding bottle (9) are in respectively and locate in bottle cover (3) on micro-fluidic chip body (1), just first holding bottle (2) and second first holding bottle (2) correspond needle body (4) that are provided with needle mouth (41) respectively, and two holding bottles are through having respectively needle body (4) with micro-fluidic pipeline (11) can form the intercommunication, first axial first end and the second end that holds bottle (2) are connected with first seal assembly and second seal assembly respectively so as to form the inside seal that first holding bottle (2) holds Receive the space, the second holds bottle (9) and is bottom seal bottle and oral area fixedly connected with first seal assembly, needle body (4) have needle mouth (41) are in first sealing condition in the first seal assembly, needle mouth (41) are in the circulation state in the inside seal accommodation space, first bottle (2), the second of holding can be followed under the effect of axial force the axial linear motion of bottle cover (3) so that needle body (4) convert the circulation state into by first sealing condition, the second seal assembly can be in be close to under the effect of axial force first seal assembly so that the material releases.
2. Microfluidic device according to claim 1, characterized in that the needle body (4) further has a second sealing condition of the needle mouth (41) within the second sealing assembly, which is also capable of switching the needle body (4) from the flow-through condition to the second sealing condition under the action of the axial force.
3. The microfluidic device according to claim 2, wherein the first sealing assembly comprises a first rubber plug (51), a protective cover (52), and the needle opening (41) is in the first rubber plug (51) when the needle body (4) is in the first sealing state; the second sealing component comprises a second rubber plug (61) and a hard gasket (62) arranged on one side, deviating from the first rubber plug (51), of the second rubber plug (61), and the needle body (4) is in the second sealing state, and the needle opening (41) is arranged in the second rubber plug (61).
4. The microfluidic device according to claim 3, wherein the first end is a throat structure, the first rubber plug (51) is a first convex rubber plug, and a convex protrusion of the first convex rubber plug is embedded in the throat structure; and/or a sealing convex ring (611) is arranged on the outer circumferential wall of the second rubber plug (61); and/or a first stop ring (21) which is convex towards the radial inner side of the first accommodating bottle body (2) is arranged on the inner circumferential wall of the second end of the first accommodating bottle body (2), and the inner circular diameter of the ring body of the first stop ring (21) is smaller than the outer circular diameter of the second rubber plug (61).
5. The microfluidic device according to claim 4, wherein the second rubber plug (61) is a second embossed rubber plug, and the shape of the embossed projection of the second embossed rubber plug can match the shape of the necking structure, so as to completely release and discharge the material in the inner sealed containing space through the needle port (41).
6. The microfluidic device according to claim 1, wherein the end of the vial sleeve (3) remote from the microfluidic chip body (1) has a second stop ring (31) protruding radially inward along it; and/or the needle opening (41) is arranged on the circumferential side wall of the needle body (4).
7. The microfluidic device according to any of claims 2 to 6, wherein there are two first receiving vials (2), and the microfluidic channel (11) has two sections that are not in communication, one of the sections of the microfluidic channel (11) being capable of communicating one of the two first receiving vials (2) with the second receiving vial (9), and the other section of the microfluidic channel (11) being capable of communicating the other of the two first receiving vials (2) with the second receiving vial (9).
8. A blending control method of a microfluidic device, which is used for controlling the microfluidic device of any one of claims 1 to 6 for realizing material blending in the microfluidic chip, and comprises the following steps:
controlling and applying axial force to a second sealing component of the first accommodating bottle body (2) and the bottom of the second accommodating bottle body (9) to enable the first accommodating bottle body (2) and the second accommodating bottle body (9) to move close to the needle body (4), so that the needle body (4) is respectively converted from the first sealing state to the circulation state;
and controlling to release the axial force applied to the bottom of the second accommodating bottle body (9) and continuously apply the axial force to the second sealing component of the first accommodating bottle body (2), releasing the axial force applied to the second sealing component before the needle body (4) corresponding to the needle body is converted from the circulation state to the second sealing state, and applying the axial force to the second sealing component again after preset time, and controlling to release the axial force applied to the second sealing component of the first accommodating bottle body (2) after the axial force is applied for preset times at intervals.
9. A blending control method of a microfluidic device, which is used for controlling the microfluidic device for realizing material blending in the microfluidic chip of claim 7, and comprises the following steps:
controlling to apply axial force to the two first containing bottle bodies (2) and the second containing bottle bodies (9) respectively provided with a second sealing component to enable the corresponding containing bottle bodies to move close to the needle body (4), and enabling the needle body (4) to be respectively converted from the first sealing state to the circulating state;
controlling to release the axial force applied to the second bottle-containing sealing assembly and continue to apply the axial force to the first bottle-containing sealing assembly to force the material therein to flow into the second bottle-containing body through the second bottle-containing body (9) until the axial force applied to the corresponding needle body (4) is released before the needle body is switched from the flow-through state to the second sealing state;
and controlling the second sealing assembly of the second containing bottle body to apply axial force to enable the second sealing assembly of the second containing bottle body to move close to the needle body (4) so as to force the material in the second containing bottle body to flow into the first containing bottle body through the second containing bottle body (9) until the axial force application to the needle body (4) is released before the needle body (4) corresponding to the second containing bottle body is converted from the flowing state to the second sealing state, and applying force to the second sealing assembly of the first containing bottle body again, so that after the axial force is applied alternately for a preset number of times, the axial force application to the first containing bottle body and the second containing bottle body is released after the needle body (4) corresponding to one of the first containing bottle body and the second containing bottle body is converted from the flowing state to the second sealing state.
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| CN113769805B (en) | 2023-01-10 |
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