CN113717837B - Detachable medicinal nucleic acid purification column - Google Patents
Detachable medicinal nucleic acid purification column Download PDFInfo
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- CN113717837B CN113717837B CN202110931988.3A CN202110931988A CN113717837B CN 113717837 B CN113717837 B CN 113717837B CN 202110931988 A CN202110931988 A CN 202110931988A CN 113717837 B CN113717837 B CN 113717837B
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- 238000001821 nucleic acid purification Methods 0.000 title claims abstract description 20
- 238000000746 purification Methods 0.000 claims abstract description 47
- 230000001681 protective effect Effects 0.000 claims description 6
- 239000012620 biological material Substances 0.000 abstract description 12
- 150000007523 nucleic acids Chemical class 0.000 abstract description 9
- 102000039446 nucleic acids Human genes 0.000 abstract description 9
- 108020004707 nucleic acids Proteins 0.000 abstract description 9
- 239000012535 impurity Substances 0.000 abstract description 4
- 238000004140 cleaning Methods 0.000 abstract description 2
- 238000000926 separation method Methods 0.000 abstract description 2
- 239000007788 liquid Substances 0.000 description 4
- 239000000463 material Substances 0.000 description 4
- 238000005119 centrifugation Methods 0.000 description 2
- 238000010586 diagram Methods 0.000 description 2
- 238000000605 extraction Methods 0.000 description 2
- 238000001914 filtration Methods 0.000 description 2
- 238000000034 method Methods 0.000 description 2
- 238000011084 recovery Methods 0.000 description 2
- 238000012360 testing method Methods 0.000 description 2
- 238000012546 transfer Methods 0.000 description 2
- 238000004804 winding Methods 0.000 description 2
- VYPSYNLAJGMNEJ-UHFFFAOYSA-N Silicium dioxide Chemical compound O=[Si]=O VYPSYNLAJGMNEJ-UHFFFAOYSA-N 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 238000004891 communication Methods 0.000 description 1
- 108010025899 gelatin film Proteins 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 238000007670 refining Methods 0.000 description 1
- 239000000741 silica gel Substances 0.000 description 1
- 229910002027 silica gel Inorganic materials 0.000 description 1
- 238000001179 sorption measurement Methods 0.000 description 1
Classifications
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
- C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
- C12N15/1017—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by filtration, e.g. using filters, frits, membranes
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01D—SEPARATION
- B01D15/00—Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
- B01D15/08—Selective adsorption, e.g. chromatography
- B01D15/10—Selective adsorption, e.g. chromatography characterised by constructional or operational features
- B01D15/18—Selective adsorption, e.g. chromatography characterised by constructional or operational features relating to flow patterns
- B01D15/1864—Selective adsorption, e.g. chromatography characterised by constructional or operational features relating to flow patterns using two or more columns
- B01D15/1871—Selective adsorption, e.g. chromatography characterised by constructional or operational features relating to flow patterns using two or more columns placed in series
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01D—SEPARATION
- B01D15/00—Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
- B01D15/08—Selective adsorption, e.g. chromatography
- B01D15/10—Selective adsorption, e.g. chromatography characterised by constructional or operational features
- B01D15/22—Selective adsorption, e.g. chromatography characterised by constructional or operational features relating to the construction of the column
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01D—SEPARATION
- B01D15/00—Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
- B01D15/08—Selective adsorption, e.g. chromatography
- B01D15/26—Selective adsorption, e.g. chromatography characterised by the separation mechanism
- B01D15/38—Selective adsorption, e.g. chromatography characterised by the separation mechanism involving specific interaction not covered by one or more of groups B01D15/265 and B01D15/30 - B01D15/36, e.g. affinity, ligand exchange or chiral chromatography
- B01D15/3804—Affinity chromatography
- B01D15/3819—Affinity chromatography of the nucleic acid-nucleic acid binding protein type
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01D—SEPARATION
- B01D36/00—Filter circuits or combinations of filters with other separating devices
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
- C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
- C12N15/1006—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers
- C12N15/101—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers by chromatography, e.g. electrophoresis, ion-exchange, reverse phase
Landscapes
- Chemical & Material Sciences (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Life Sciences & Earth Sciences (AREA)
- Analytical Chemistry (AREA)
- Biomedical Technology (AREA)
- Genetics & Genomics (AREA)
- Organic Chemistry (AREA)
- Wood Science & Technology (AREA)
- Biotechnology (AREA)
- General Engineering & Computer Science (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Zoology (AREA)
- Molecular Biology (AREA)
- Biochemistry (AREA)
- Crystallography & Structural Chemistry (AREA)
- Microbiology (AREA)
- Plant Pathology (AREA)
- General Health & Medical Sciences (AREA)
- Biophysics (AREA)
- Physics & Mathematics (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
Abstract
The invention discloses a detachable medicinal nucleic acid purification column, which belongs to the technical field of nucleic acid purification and comprises a placing frame and a column body, wherein the column body is arranged on the placing frame, and comprises a first purification column, a second purification column and a third purification column; the upper part of the second purifying column is clamped with the lower part of the first purifying column, and the upper part of the third purifying column is clamped with the lower part of the second purifying column; the nucleic acid purification column is divided into a first purification column, a second purification column and a third purification column, is convenient to detach and install, and is continuous in operation, so that excessive biological material transferring steps are avoided, and pollution is prevented; the column body can be erected on the placing rack, so that the use is convenient; the filter paper tube is arranged in the first purifying column of the nucleic acid purifying column, so that larger impurities in the freshly crushed biological material can be filtered, and the next step of separation and purification is facilitated; the filter paper tube is arranged in the limiting tube, the limiting tube is detachable, the filter paper tube is convenient to place, and cleaning and replacement are convenient.
Description
Technical Field
The invention relates to the technical field of nucleic acid purification, in particular to a detachable medicinal nucleic acid purification column.
Background
Nucleic acid extraction and purification are the basis of molecular biology tests, and a nucleic acid purification method is an important factor influencing the quality of extracted nucleic acid and is also a key to success or failure of downstream molecular biology tests. Nucleic acid purification is commonly performed in laboratories using nucleic acid purification columns.
The nucleic acid purifying column can be used for extracting and refining DNA/RNA of various materials, and has the characteristics of simple operation, high recovery rate, stable performance and the like. The nucleic acid purification column adopts a silica gel film as a specific adsorption material of nucleic acid, and basically does not adsorb other biological materials, so that the maximum recovery of DNA/RNA in a sample can be ensured, and other impurities can be removed. The existing nucleic acid purification columns are generally selected according to different extraction requirements, and the transfer of materials can be involved in the operation process, so that the result of nucleic acid purification can be influenced by slightly careless operation, and the operation of transferring materials needs to be reduced as much as possible.
Disclosure of Invention
The invention aims to provide a detachable medicinal nucleic acid purification column, which is used for purifying nucleic acid of biological materials through a first purification column, a second purification column and a third purification column which are convenient to detach and install so as to reduce transfer steps in the purification process.
The aim of the invention can be achieved by the following technical scheme: the detachable medicinal nucleic acid purification column comprises a placing frame and a column body, wherein the column body is arranged on the placing frame and comprises a first purification column, a second purification column and a third purification column; the upper part of the second purifying column is clamped with the lower part of the first purifying column, and the upper part of the third purifying column is clamped with the lower part of the second purifying column;
further, the placing frame comprises a bottom plate and a top plate, four corners of the bottom plate and the top plate are respectively connected through supporting rods, a frame plate is arranged between the bottom plate and the top plate, and four corners of the frame plate are respectively fixedly connected with the four supporting rods; the frame plate and the top plate are provided with notches for erecting the column body;
further, the first purification column comprises a first outer tube, a first collecting pipe is arranged at the bottom of the first outer tube, and a circular unfolded annular flange is arranged at the top of the first outer tube; the top end of the first outer tube is provided with a limiting cylinder, the limiting cylinder comprises a limiting ring, a middle position ring and a base, the limiting cylinder is fixedly connected through three connecting rods which are uniformly arranged in the circumferential direction, and the middle position ring is arranged between the limiting ring and the base;
the outer diameters of the middle ring and the base are smaller than the inner diameter of the first outer pipe, and the difference is D1, D1=1.5-4 mm; the outer diameter of the limiting ring is larger than the inner diameter of the first outer pipe, and the limiting ring is erected on the annular flange of the first outer pipe; the limiting cylinder is internally provided with a filter paper cylinder, and the filter paper cylinder is formed by winding rectangular filter paper; the base is provided with an annular limiting groove corresponding to the filter paper cylinder, and one end of the filter paper cylinder is clamped in the limiting groove;
further, the second purification column comprises a second outer tube; the upper end of the second outer tube is provided with a butterfly plate, the lower end of the second outer tube is provided with a clamping tube, and the lower end of the clamping tube is provided with a second collecting pipe; the bottom of the second outer tube is provided with a first filter plate which is fixed through a first pressing ring; air grooves are formed in the clamping pipe and the second collecting pipe and used for balancing air pressure;
the inner diameter of the second outer tube is the same as that of the first collecting tube, so that the second outer tube is convenient to clamp and fix; the second outer tube is also provided with a piston rod in a matched mode, the piston rod comprises a push rod, and the bottom of the push rod is fixedly provided with a piston head; the diameter of the piston head is larger than the inner diameter of the second outer tube, and the difference is D2, and D2=0.5-0.8 mm;
further, the third purification column comprises a third outer tube, an inner tube is sleeved in the third outer tube, and the inner diameter of the third outer tube is the same as the outer diameter of the inner tube; the lower end of the inner tube is provided with a third collecting pipe, and the upper end of the inner tube is provided with a lantern ring; the bottom of the inner tube is provided with a second filter plate and is fixed through a second pressing ring; when the second purifying column is connected with the third purifying column, the clamping pipe is clamped with the lantern ring, and the second collecting pipe stretches into the inner pipe; the upper end of the third outer tube is connected with a plug through a soft belt, when the third purification column is arranged in a centrifugal machine for operation, the inner tube is plugged to prevent liquid leakage, and a protective shell is clamped outside the plug to prevent the column from being polluted when other operation steps are carried out;
the first pressing ring and the second pressing ring can not adsorb most biological molecules, and the second filter plate can adsorb nucleic acid molecules; the detachable medicinal nucleic acid purification column can be provided with different specifications according to the nucleic acid purification requirement.
The invention has the beneficial effects that:
1. the nucleic acid purification column is divided into a first purification column, a second purification column and a third purification column, is convenient to detach and install, and is continuous in operation, so that excessive biological material transferring steps are avoided, and pollution is prevented; the column body can be erected on the placing frame, and the use is convenient.
2. The filter paper tube is arranged in the first purifying column of the nucleic acid purifying column, so that larger impurities in the freshly crushed biological material can be filtered, and the next step of separation and purification is facilitated; the filter paper tube is arranged in the limiting tube, the limiting tube is detachable, the filter paper tube is convenient to place, and cleaning and replacement are convenient.
3. The second outer tube can collect the biological material purified by the first purifying column, at the moment, the column body is taken down from the placing frame, the first purifying column is disassembled, and the biological material in the first outer tube can be further purified and sent into the inner tube by matching with the piston rod; the clamping pipe and the collecting pipe are provided with air grooves, so that air pressure can be balanced when the piston rod is pushed.
4. After the operation of the second purification column part is completed, when the third purification column is placed in the centrifuge for operation, the plug can plug the inner tube to prevent liquid leakage, and the plug is also clamped with the protective shell to prevent the column from being polluted when other operation steps are performed.
Drawings
The invention is further described below with reference to the accompanying drawings.
FIG. 1 is a schematic diagram of the structure of the present invention;
FIG. 2 is a schematic view of a rack of the present invention;
FIG. 3 is a first purification column exploded view of the present invention;
FIG. 4 is a schematic view of a stopper rod according to the present invention;
FIG. 5 is a schematic view of a second outer tube portion of the present invention;
FIG. 6 is a schematic diagram of the second purification column and third purification column of the present invention in combination;
FIG. 7 is a schematic representation of a third purification column of the present invention;
FIG. 8 is a disassembled view of a third purification column of the present invention;
fig. 9 is a schematic view of a piston rod according to the present invention.
In the figure: 1. a placing rack; 2. a first purification column; 3. a second purification column; 4. a third purification column; 11. a bottom plate; 12. a frame plate; 13. a top plate; 14. a support rod; 21. a first outer tube; 22. a first header; 23. a limiting cylinder; 24. a filter paper tube; 231. a limiting ring; 232. a median ring; 233. a base; 234. a connecting rod; 235. a limit groove; 31. a second outer tube; 32. a butterfly plate; 33. a first filter plate; 34. a first clamping ring; 35. a clamping pipe; 36. a second header; 37. an air tank; 38. a piston rod; 381. a push rod; 382. a piston head; 41. a third outer tube; 42. an inner tube; 43. a third header; 44. a collar; 45. a second filter plate; 46. a second clamping ring; 47. a plug; 48. and a protective shell.
Detailed Description
The following description of the embodiments of the present invention will be made clearly and completely with reference to the accompanying drawings, in which it is apparent that the embodiments described are only some embodiments of the present invention, but not all embodiments. All other embodiments, which can be made by those skilled in the art based on the embodiments of the invention without making any inventive effort, are intended to be within the scope of the invention.
Referring to fig. 1-9, the invention is a detachable medicinal nucleic acid purification column, comprising a rack 1 and a column body, wherein the column body is arranged on the rack 1, and comprises a first purification column 2, a second purification column 3 and a third purification column 4; the upper part of the second purifying column 3 is clamped with the lower part of the first purifying column 2, and the upper part of the third purifying column 4 is clamped with the lower part of the second purifying column 3; the rack 1 comprises a bottom plate 11 and a top plate 13, four corners of the bottom plate 11 and the top plate 13 are connected through supporting rods 14, a rack plate 12 is arranged between the bottom plate 11 and the top plate 13, and the four corners of the rack plate 12 are fixedly connected with the four supporting rods 14 respectively; the frame plate 12 and the top plate 13 are provided with notches for erecting the column;
the first purification column 2 comprises a first outer tube 21, a first collecting pipe 22 is arranged at the bottom of the first outer tube 21, and a circular unfolded annular flange is arranged at the top of the first outer tube 21; the top end of the first outer tube 21 is provided with a limiting cylinder 23, the limiting cylinder 23 comprises a limiting ring 231, a middle ring 232 and a base 233, the limiting cylinder is fixedly connected through three connecting rods 234 which are uniformly arranged in the circumferential direction, and the middle ring 232 is arranged between the limiting ring 231 and the base 233; the outer diameters of the middle ring 232 and the base 233 are smaller than the inner diameter of the first outer tube 21; the outer diameter of the limiting ring 231 is larger than the inner diameter of the first outer tube 21, and the limiting ring is erected on the annular flange of the first outer tube 21; the limiting cylinder 23 is internally provided with a filter paper cylinder 24, and the filter paper cylinder 24 is formed by winding rectangular filter paper; the base 233 is provided with a limit groove 235 corresponding to the filter paper cylinder 24, and one end of the filter paper cylinder 24 is clamped in the limit groove 235;
the second purification column 3 comprises a second outer tube 31; the upper end of the second outer pipe 31 is provided with a butterfly plate 32, the lower end is provided with a clamping pipe 35, and the lower end of the clamping pipe 35 is provided with a second collecting pipe 36; the bottom of the second outer tube 31 is provided with a first filter plate 33, and the first filter plate 33 is fixed by a first clamping ring 34; the clamping pipe 35 and the second collecting pipe 36 are provided with air grooves 37 for balancing air pressure; the inner diameter of the second outer pipe 31 is the same as that of the first collecting pipe 22, so that clamping and fixing are facilitated; the second outer tube 31 is also provided with a piston rod 38 in a matched manner, the piston rod 38 comprises a push rod 381, and a piston head 382 is fixedly arranged at the bottom of the push rod 381; the diameter of piston head 382 is greater than the inner diameter of second outer tube 31;
the third purification column 4 comprises a third outer tube 41, an inner tube 42 is sleeved in the third outer tube 41, and the inner diameter of the third outer tube 41 is the same as the outer diameter of the inner tube 42; the lower end of the inner pipe 42 is provided with a third collecting pipe 43, and the upper end is provided with a collar 44; the bottom of the inner tube 42 is provided with a second filter plate 45 and is fixed by a second pressing ring 46; when the second purifying column 3 is connected with the third purifying column 4, the clamping pipe 35 is clamped with the lantern ring 44, and the second collecting pipe 36 extends into the inner pipe 42; the upper end of the third outer tube 41 is connected with a plug 47, when the third purification column 4 is arranged in the centrifuge for operation, the inner tube 42 is plugged to prevent liquid leakage, and a protective shell 48 is clamped outside the plug 47 to prevent the column from being polluted when other operation steps are carried out.
The working principle of the invention is as follows: firstly, assembling a first purifying column 2, a second purifying column 3 and a third purifying column 4 into a complete column, erecting the column on a rack 1, arranging a circular unfolded annular flange on the top of a first outer tube 21 and erecting the circular unfolded annular flange on a top plate 13, and erecting a butterfly plate 32 on a rack plate 12;
crushing the cell biological material, adding the crushed cell biological material into a first purification column 2, filtering larger impurities by a filter paper cylinder 24, and flowing the crude extracted biological material into a second outer pipe 31 along a first collecting pipe 22; taking off the column body, taking off the part of the first purifying column 2, plugging the piston rod 38 into the second outer tube 31, pressing the push rod 381, filtering the crude biological material by the first filter plate 33 to obtain a middle extract, and flowing the middle extract into the inner tube 42 along the second collecting tube 36; removing the second purifying column 3, removing the protective shell 48, plugging the plug 47 into the inner tube 42, and placing the third purifying column 4 into a centrifuge for centrifugation; the nucleic acid molecules in the extract are adsorbed by the second filter plate 45, and further separated and purified, and the rest liquid enters the bottom of the third outer tube 41 through centrifugation.
In the description of the present invention, it should be understood that the terms "upper," "lower," "left," "right," and the like indicate an orientation or a positional relationship based on that shown in the drawings, and are merely for convenience of description and for simplifying the description, and do not indicate or imply that the apparatus or element in question must have a specific orientation, as well as a specific orientation configuration and operation, and thus should not be construed as limiting the present invention. Furthermore, the terms "first," "second," and the like, are used for descriptive purposes only and are not to be construed as indicating or implying a relative importance or implicitly indicating the number of technical features indicated. Thus, a feature defining "a first" or "a second" may explicitly or implicitly include one or more such feature. In the description of the present invention, unless otherwise indicated, the meaning of "a plurality" is two or more.
In the description of the present invention, it should be noted that, unless explicitly specified and limited otherwise, the terms "mounted," "connected," and the like are to be construed broadly and may be, for example, fixedly connected, detachably connected, or integrally connected; can be mechanically or electrically connected; can be directly connected or indirectly connected through an intermediate medium, and can be communication between two elements. The specific meaning of the above terms in the present invention will be understood in specific cases by those of ordinary skill in the art.
The foregoing describes one embodiment of the present invention in detail, but the description is only a preferred embodiment of the present invention and should not be construed as limiting the scope of the invention. All equivalent changes and modifications within the scope of the present invention are intended to be covered by the present invention.
Claims (1)
1. The detachable medicinal nucleic acid purification column comprises a placing frame (1) and a column body, wherein the column body is erected on the placing frame (1), and is characterized in that the column body comprises a first purification column (2), a second purification column (3) and a third purification column (4); the first purifying column (2), the second purifying column (3) and the third purifying column (4) are sequentially clamped;
the rack (1) comprises a bottom plate (11) and a top plate (13), wherein four corners of the bottom plate (11) and the top plate (13) are respectively connected through a supporting rod (14), and a rack plate (12) is connected between the bottom plate (11) and the top plate (13) through the supporting rods (14); the frame plate (12) and the top plate (13) are provided with notches;
the first purification column (2) comprises a first outer tube (21), a first collecting pipe (22) is arranged at the bottom of the first outer tube (21), and an annular flange is arranged at the top of the first outer tube (21); the top end of the first outer tube (21) is provided with a limiting cylinder (23), the limiting cylinder (23) comprises a limiting ring (231), a middle ring (232) and a base (233), and the limiting cylinder is fixedly connected through a plurality of connecting rods (234) which are uniformly arranged in the circumferential direction;
the outer diameters of the middle ring (232) and the base (233) are smaller than the inner diameter of the first outer tube (21); the outer diameter of the limiting ring (231) is larger than the inner diameter of the first outer tube (21);
a filter paper tube (24) is arranged in the limit tube (23); the base (233) is provided with a limit groove (235), and one end of the filter paper cylinder (24) is clamped in the limit groove (235);
the second purification column (3) comprises a second outer tube (31); the upper end of the second outer pipe (31) is provided with a butterfly plate (32), the lower end of the second outer pipe is provided with a clamping pipe (35), and the lower end of the clamping pipe (35) is provided with a second collecting pipe (36); the bottom of the second outer pipe (31) is provided with a first filter plate (33), and the first filter plate (33) is fixed through a first pressing ring (34);
the clamping pipe (35) and the second collecting pipe (36) are provided with air grooves (37);
the second outer tube (31) is provided with a piston rod (38) in a matching way, the piston rod (38) comprises a push rod (381), and a piston head (382) is fixedly arranged at the bottom of the push rod (381); the diameter of the piston head (382) is greater than the inner diameter of the second outer tube (31);
the third purification column (4) comprises a third outer tube (41), and an inner tube (42) is sleeved in the third outer tube (41); the lower end of the inner pipe (42) is provided with a third collecting pipe (43), and the upper end is provided with a collar (44); the bottom of the inner tube (42) is provided with a second filter plate (45) and is fixed through a second pressing ring (46);
the upper end of the third outer tube (41) is connected with a plug (47), and a protective shell (48) is clamped outside the plug (47).
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202110931988.3A CN113717837B (en) | 2021-08-13 | 2021-08-13 | Detachable medicinal nucleic acid purification column |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202110931988.3A CN113717837B (en) | 2021-08-13 | 2021-08-13 | Detachable medicinal nucleic acid purification column |
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| Publication Number | Publication Date |
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| CN113717837A CN113717837A (en) | 2021-11-30 |
| CN113717837B true CN113717837B (en) | 2023-10-03 |
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Families Citing this family (1)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN115232808A (en) * | 2022-06-09 | 2022-10-25 | 通用生物(安徽)股份有限公司 | Preparation device and application of novel magnetic bead method purified DNA (deoxyribonucleic acid) matching reagent |
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