CN113533708B - Diabetes screening device based on urine acetone detection - Google Patents
Diabetes screening device based on urine acetone detection Download PDFInfo
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- CN113533708B CN113533708B CN202110946307.0A CN202110946307A CN113533708B CN 113533708 B CN113533708 B CN 113533708B CN 202110946307 A CN202110946307 A CN 202110946307A CN 113533708 B CN113533708 B CN 113533708B
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Abstract
The invention discloses a diabetes screening device based on urine acetone detection, which comprises a gas carrying bottle, a sampling tube, a flowmeter, a headspace bottle, a sampling pump, a detection unit, a power supply unit, a monitoring unit and a display unit; the invention utilizes the detection unit to carry out qualitative and quantitative detection on acetone in the urine overhead, and then carries out diabetes screening by comparing with the threshold value, thereby being capable of replacing the invasive method for screening diabetes by blood sugar. The technical method has the advantages of no wound, accuracy, high acceptance and the like.
Description
Technical Field
The invention relates to the fields of sensor technology, biomedical engineering and medical and health, in particular to a diabetes screening device based on urine acetone detection.
Background
Acetone is a common volatile organic compound in urine and is also a biomarker in urine of diabetics. Compared with healthy people, diabetes patients have serious insulin deficiency or cannot play a role, cause sugar metabolism disorder, aggravate fat decomposition acceleration, generate acetone, acetoacetic acid and beta-hydroxybutyric acid, reflect obvious rise of urine or acetone in expiration, and the expiration sample is easily influenced by oral cavity and digestive tract bacteria, and the urine can be collected and stored in a sterile mode. Therefore, the diabetes screening can be completed by detecting the change of the acetone content in urine.
The conventional clinical uretdione (acetone and acetoacetic acid) detection uses sodium nitrosoferricyanide test paper, the sensitivity to acetoacetic acid is only 50-100mg/L, the sensitivity to acetone is only 400-700mg/L, and the two substances are extremely volatile, so that the accuracy of the detection result is affected.
Disclosure of Invention
The technical solution of the invention is as follows: the diabetes blood sugar detection method is invasive and the pretreatment process of the sample is complex; the invention provides a novel diabetes screening technology based on urine headspace acetone detection, which realizes noninvasive and accurate screening of diabetes.
The solution of the invention is as follows: the diabetes screening device based on urine acetone detection comprises a gas carrying bottle 1, a sampling tube 2, a flowmeter a3, a headspace bottle 4, a flowmeter b5, a sampling pump 6, a detection unit 7, a power supply unit 8, a monitoring unit 9 and a display unit 10; carrier gas bottle 1 links to each other with flowmeter a3 through sampling pipe 2, flowmeter a3 links to each other with headspace bottle 4 through sampling pipe 2, headspace bottle 4 is connected with flowmeter b5 and detecting element 7 respectively through tee bend sampling pipe 2, flowmeter b5 links to each other with sampling pump 6 through sampling pipe 2, simultaneously headspace bottle 4 links to each other with detecting element 7 through sampling pipe 2, power supply unit 8 links to each other with sampling pump 6, detecting element 7, monitor unit 9 and display element 10 respectively, monitor unit 9 links to each other with sampling pump 6, detecting element 7 and display element 10 respectively.
The material of the bottle body of the headspace bottle 4 is glass or stainless steel, the bottle body is controlled at constant temperature, and the material of the bottle stopper sealing gasket is polytetrafluoroethylene.
The detection unit 7 is an acetone sensitive component, or is an acetone sensitive micro sensor or sensor array, or is a spectrum type and mass spectrum type sensing instrument, or is a device combining the component and the chromatograph.
The gas carrying bottle 1 is filled with high-purity air, high-purity nitrogen or high-purity helium which does not contain acetone.
The power supply unit 8 is a battery or mains supply.
The sampling tube 2 is made of polytetrafluoroethylene material, and is accompanied by heat at the temperature of 50-100 ℃.
The flow meter a3 and the flow meter b5 can monitor/regulate the gas flow in the pipeline.
The monitoring unit 9 precisely controls and synchronizes the temperature of the headspace bottle 4, the sampling pump 6, the flowmeter a3, the flowmeter b5, the detection unit 7 and the display unit 10 in a time domain, and realizes diabetes screening, and the specific process is as follows:
① The urine sample is taken and placed in the headspace bottle 4, the constant temperature is kept, the flow rate of the flowmeter a3 is set to be larger than the flow rate of the flowmeter b5, and the air in the headspace bottle 4 is expelled and filled with carrier gas.
② The flow meter a3 and the flow meter b5 are set to 0, and the acetone in the urine is waited for reaching the gas-liquid balance in the headspace bottle.
③ The flow rate of the flowmeter a3 is set to be larger than the flow rate of the flowmeter b5, the headspace acetone is transmitted to the detection unit 7 by utilizing the flow rate difference value, the acetone concentration is measured, the acetone concentration is compared with a threshold value set by instrument software, and if the acetone concentration is larger than the threshold value, the diabetes is judged, and the result is transmitted to the display unit 10.
Compared with the prior art, the invention has the following differences and advantages:
① Blood sugar screening methods for diabetes require blood drawing to detect sugar content, are invasive techniques, and the blood sample processing process is complex and time-consuming. The technology of the invention realizes diabetes screening through the detection of VOCs in urine, is a noninvasive technology, and has simple and rapid sample pretreatment.
② The diabetes urone screening method generally uses chemical test paper to detect acetone and acetoacetic acid in urine, has low sensitivity and poor accuracy, and the two substances are easy to volatilize, so that the error of detection results is easy to cause. The technology of the invention utilizes the headspace bottle to ensure that the acetone reaches gas-liquid balance, and then detects the concentration of gaseous acetone, thereby having high accuracy.
Drawings
Fig. 1 is a schematic diagram of a diabetes screening device based on urine acetone detection of the present invention.
In the figure, a 1-gas carrying bottle, a 2-sampling tube, a 3-flowmeter a, a 4-headspace bottle, a 5-flowmeter b, a 6-sampling pump, a 7-detection unit, an 8-power supply unit, a 9-monitoring unit and a 10-display unit.
Detailed Description
As shown in fig. 1, the diabetes screening device based on urine acetone detection comprises a gas carrying bottle 1, a sampling tube 2, a flowmeter a3, a headspace bottle 4, a flowmeter b5, a sampling pump 6, a detection unit 7, a power supply unit 8, a monitoring unit 9 and a display unit 10; carrier gas bottle 1 links to each other with flowmeter a3 through sampling pipe 2, flowmeter a3 links to each other with headspace bottle 4 through sampling pipe 2, headspace bottle 4 is connected with flowmeter b5 and detecting element 7 respectively through tee bend sampling pipe 2, flowmeter b5 links to each other with sampling pump 6 through sampling pipe 2, simultaneously headspace bottle 4 links to each other with detecting element 7 through sampling pipe 2, power supply unit 8 links to each other with sampling pump 6, detecting element 7, monitor unit 9 and display element 10 respectively, monitor unit 9 links to each other with sampling pump 6, detecting element 7 and display element 10 respectively.
The headspace bottle 4 is made of glass or stainless steel, and the bottle stopper sealing gasket is made of polytetrafluoroethylene, so that the introduction of pollution volatile organic compounds, in particular acetone, is avoided; the bottle body is controlled by constant temperature, so that the constant distribution coefficient of acetone in the headspace and urine is ensured each time.
The air-carrying bottle 1 is filled with high-purity air without acetone, or high-purity nitrogen or high-purity helium, and is used for conveying the air-state acetone on the top of the urine to the detection unit 7. The specific carrier gas selection criteria will depend on the requirements of the detector in the detection unit.
The power supply unit 8 is a battery or mains supply, and when the detector is a micro sensor, the power supply unit 8 may select a battery, and if it is a large-scale sensing instrument of the spectrum or mass spectrum type, a larger battery pack or mains supply may be selected.
The sampling tube 2 is made of polytetrafluoroethylene, so that the introduction of pollution volatile organic compounds, particularly acetone, is avoided, and meanwhile, the heat treatment is carried out at 50-100 ℃ to reduce the adsorption of acetone in carrier gas.
The flow meters a3 and b5 can monitor/regulate the flow of gas in the pipeline, typically using a mass flow meter or a float flow meter.
The monitoring unit 9 precisely controls and synchronizes the temperature of the headspace bottle 4, the sampling pump 6, the flow meter a3, the flow meter b5, the detection unit 7 and the display unit 10 in the time domain, so that the diabetes screening is realized, and the specific diabetes screening process is as follows:
① Taking a urine sample of a subject in the morning, placing the urine sample in a headspace bottle 4, placing the headspace bottle 4 in a heat preservation cavity to keep constant temperature, setting the flow rate of a flowmeter a3 to be larger than the flow rate of a flowmeter b5, expelling air in the headspace bottle 4, and filling carrier gas.
② After 30 seconds, the flow meter a3 and the flow meter b5 are set to 0 through the control unit 9, and the headspace bottle is kept stand for more than 40 minutes, so that the acetone in the urine reaches gas-liquid balance.
③ The flow rate of the flowmeter a3 is set to be larger than the flow rate of the flowmeter b5 again, the headspace acetone is transmitted to the detection unit 7 by utilizing the flow rate difference value, the acetone concentration is measured, and when the acetone concentration is larger than the threshold value set by instrument software, the diabetes is judged, and the result is transmitted to the display unit 10.
The detection unit 7 is an acetone sensitive component, or is an acetone sensitive micro sensor or sensor array, or is a spectrum type and mass spectrum type sensing instrument, or is a device combining the component and the chromatograph. Such as fourier infrared spectrometer, chemical ionization mass spectrometer, electrospray extraction ionization mass spectrometer, photoionization mass spectrometer, etc. for organic matter detection, wherein the chemical ionization mass spectrometer comprises proton transfer reaction mass spectrometer, ion-molecule reaction mass spectrometer, organic matter doping auxiliary photoionization mass spectrometer, and the combination device of the mass spectrometer and chromatograph.
Portions of this specification, not specifically described herein, are well known in the art.
While the invention has been described with respect to certain preferred embodiments, it will be apparent to those skilled in the art that various changes and substitutions can be made herein without departing from the scope of the invention as defined by the appended claims.
Claims (3)
1. Diabetes screening device based on urine acetone detects, characterized in that includes: the device comprises a gas carrying bottle (1), a sampling tube (2), a flowmeter a (3), a headspace bottle (4), a flowmeter b (5), a sampling pump (6), a detection unit (7), a power supply unit (8), a monitoring unit (9) and a display unit (10); the carrier gas bottle (1) is connected with the flow meter a (3) through the sampling tube (2), the flow meter a (3) is connected with the headspace bottle (4) through the sampling tube (2), the headspace bottle (4) is respectively connected with the flow meter b (5) and the detection unit (7) through the tee sampling tube (2), the flow meter b (5) is connected with the sampling pump (6) through the sampling tube (2), meanwhile, the headspace bottle (4) is connected with the detection unit (7) through the sampling tube (2), the power supply unit (8) is respectively connected with the sampling pump (6), the detection unit (7), the monitoring unit (9) and the display unit (10), and the monitoring unit (9) is respectively connected with the sampling pump (6), the detection unit (7) and the display unit (10);
The bottle body of the headspace bottle (4) is made of glass or stainless steel, and the bottle body is controlled at constant temperature, so that the distribution coefficient of acetone in the headspace and urine is constant each time, and the bottle stopper sealing gasket is made of polytetrafluoroethylene, so that the introduction of pollution volatile organic matters is avoided; the flowmeter a (3) and the flowmeter b (5) are mass flowmeters or float flowmeters; the detection unit (7) is an acetone-sensitive component, or is an acetone-sensitive micro sensor or sensor array, or is a spectrum sensing instrument and a mass spectrum sensing instrument, or is a device combining the component and the chromatograph;
The carrier gas bottle (1) is filled with high-purity air without acetone, or high-purity nitrogen or high-purity helium, and is used for conveying the air acetone on the top of the urine to the detection unit (7); the sampling tube (2) is made of polytetrafluoroethylene material, and is subjected to heat treatment at 50-100 ℃ to reduce the adsorption of acetone in carrier gas;
The monitoring unit (9) accurately controls and synchronizes the temperature of the headspace bottle (4), the sampling pump (6), the flowmeter a (3), the flowmeter b (5), the detection unit (7) and the display unit (10) in a time domain, and realizes diabetes screening, and the specific process is as follows:
① Placing a urine sample in a headspace bottle (4) and keeping the temperature constant, setting the flow rate of a flowmeter a (3) to be larger than the flow rate of a flowmeter b (5), expelling air in the headspace bottle (4), and filling carrier gas;
② The flow meter a (3) and the flow meter b (5) are set to be 0, and the balance of the acetone in the urine in the gas-liquid in the headspace bottle is waited;
③ Setting the flow rate of the flow meter a (3) to be larger than that of the flow meter b (5), transmitting the headspace acetone to the detection unit (7) by utilizing the flow rate difference value, measuring the acetone concentration, comparing the acetone concentration with a threshold value set by instrument software, judging that the patient is diabetic if the acetone concentration is larger than the threshold value, and transmitting the result to the display unit (10).
2. The diabetes screening device based on urine acetone detection according to claim 1, wherein: the power supply unit (8) is a battery or mains supply.
3. The diabetes screening device based on urine acetone detection according to claim 1, wherein: the flow meter a (3) and the flow meter b (5) are capable of monitoring/regulating the flow of gas in the pipeline.
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| CN202110946307.0A CN113533708B (en) | 2021-08-18 | 2021-08-18 | Diabetes screening device based on urine acetone detection |
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| CN202110946307.0A CN113533708B (en) | 2021-08-18 | 2021-08-18 | Diabetes screening device based on urine acetone detection |
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| CN114236108A (en) * | 2021-12-20 | 2022-03-25 | 中国科学院合肥物质科学研究院 | Device and method for detecting helicobacter pylori based on ammonia in expired air |
| CN115308409A (en) * | 2022-08-31 | 2022-11-08 | 中国科学院合肥物质科学研究院 | Bacteria identification device and method based on volatile matter detection |
| CN118190529B (en) * | 2024-05-15 | 2024-09-24 | 宁波华仪宁创智能科技有限公司 | Liquid sampling method and device |
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| CN102384951A (en) * | 2011-08-19 | 2012-03-21 | 华南理工大学 | Method by utilizing all-volatility headspace gas chromatography to measure content of alkoxy |
| CN106066395A (en) * | 2016-07-08 | 2016-11-02 | 杭州同泉物联网技术有限公司 | A kind of urine detection method and device thereof |
| CN215866706U (en) * | 2021-08-18 | 2022-02-18 | 中国科学院合肥物质科学研究院 | Diabetes screening device based on urine acetone detection |
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| JP6817808B2 (en) * | 2016-12-26 | 2021-01-20 | 太陽誘電株式会社 | Biological monitoring device and urine analysis method |
| CN107655885A (en) * | 2017-10-11 | 2018-02-02 | 吉红亮 | A kind of division of endocrinology's diabetes fast testing apparatus |
| CN109856381A (en) * | 2019-03-26 | 2019-06-07 | 孙京喜 | A kind of division of endocrinology's diabetes fast testing apparatus |
| CN110308278A (en) * | 2019-08-13 | 2019-10-08 | 王增梅 | A kind of diabetes internal medicine device for fast detecting |
| CN112924533B (en) * | 2019-12-06 | 2021-12-28 | 中国科学院大连化学物理研究所 | A kind of method for online detection of exhaled breath acetone |
| CN112394172A (en) * | 2020-12-03 | 2021-02-23 | 长春理工大学 | Acetone monitoring device for exhaled gas of diabetic patient |
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| CN102384951A (en) * | 2011-08-19 | 2012-03-21 | 华南理工大学 | Method by utilizing all-volatility headspace gas chromatography to measure content of alkoxy |
| CN106066395A (en) * | 2016-07-08 | 2016-11-02 | 杭州同泉物联网技术有限公司 | A kind of urine detection method and device thereof |
| CN215866706U (en) * | 2021-08-18 | 2022-02-18 | 中国科学院合肥物质科学研究院 | Diabetes screening device based on urine acetone detection |
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